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| micrornasSummarySummary: Small regulatory, ANTISENSE RNAs, 21-25 nucleotides in length that repress the translation (TRANSLATION, GENETIC) of homologous target RNA. The process by which they are generated is related to the process that produces small interfering RNAs (RNA, SMALL INTERFERING). The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans are a class of miRNAs Top Publications
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MicroRNA signatures in human cancersGeorge A Calin
Department of Molecular Virology, Immunology and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210, USA
Nat Rev Cancer 6:857-66. 2006..In addition, profiling has been exploited to identify miRNA genes that might represent downstream targets of activated oncogenic pathways, or that target protein-coding genes involved in cancer...
MicroRNAs: target recognition and regulatory functionsDavid P Bartel
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Cell 136:215-33. 2009b>MicroRNAs (miRNAs) are endogenous approximately 23 nt RNAs that play important gene-regulatory roles in animals and plants by pairing to the mRNAs of protein-coding genes to direct their posttranscriptional repression...
MicroRNAs: genomics, biogenesis, mechanism, and functionDavid P Bartel
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell 116:281-97. 2004b>MicroRNAs (miRNAs) are endogenous approximately 22 nt RNAs that can play important regulatory roles in animals and plants by targeting mRNAs for cleavage or translational repression...
Most mammalian mRNAs are conserved targets of microRNAsRobin C Friedman
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Genome Res 19:92-105. 2009b>MicroRNAs (miRNAs) are small endogenous RNAs that pair to sites in mRNAs to direct post-transcriptional repression...
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targetsBenjamin P Lewis
Cell 120:15-20. 2005We predict regulatory targets of vertebrate microRNAs (miRNAs) by identifying mRNAs with conserved complementarity to the seed (nucleotides 2-7) of the miRNA...
miRBase: tools for microRNA genomicsSam Griffiths-Jones
Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, UK
Nucleic Acids Res 36:D154-8. 2008..miRBase is available at http://microrna.sanger.ac.uk/...
Mammalian microRNAs predominantly act to decrease target mRNA levelsHuili Guo
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Nature 466:835-40. 2010b>MicroRNAs (miRNAs) are endogenous approximately 22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression...
Origins and Mechanisms of miRNAs and siRNAsRichard W Carthew
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
Cell 136:642-55. 2009..Two primary categories of these small RNAs--short interfering RNAs (siRNAs) and microRNAs (miRNAs)--act in both somatic and germline lineages in a broad range of eukaryotic species to regulate ..
MicroRNA expression profiles classify human cancersJun Lu
Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02141, USA
Nature 435:834-8. 2005Recent work has revealed the existence of a class of small non-coding RNA species, known as microRNAs (miRNAs), which have critical functions across various biological processes...
Circulating microRNAs as stable blood-based markers for cancer detectionPatrick S Mitchell
Divisions of Human Biology, Clinical Research, and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 105:10513-8. 2008..b>MicroRNAs (miRNAs) are small ( approximately 22 nt) regulatory RNAs that are frequently dysregulated in cancer and have ..
Regulation of mRNA translation and stability by microRNAsMarc Robert Fabian
Department of Biochemistry and Goodman Cancer Research Center, McGill University, Montreal, Quebec, H3G 1Y6, Canada
Annu Rev Biochem 79:351-79. 2010b>MicroRNAs (miRNAs) are small noncoding RNAs that extensively regulate gene expression in animals, plants, and protozoa...
Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cellsAlexander Marson
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell 134:521-33. 2008b>MicroRNAs (miRNAs) are crucial for normal embryonic stem (ES) cell self-renewal and cellular differentiation, but how miRNA gene expression is controlled by the key transcriptional regulators of ES cells has not been established...
The impact of microRNAs on protein outputDaehyun Baek
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Nature 455:64-71. 2008b>MicroRNAs are endogenous approximately 23-nucleotide RNAs that can pair to sites in the messenger RNAs of protein-coding genes to downregulate the expression from these messages...
Mammalian microRNAs: experimental evaluation of novel and previously annotated genesH Rosaria Chiang
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Genes Dev 24:992-1009. 2010b>MicroRNAs (miRNAs) are small regulatory RNAs that derive from distinctive hairpin transcripts...
Oncomirs - microRNAs with a role in cancerAurora Esquela-Kerscher
Yale University, Department of Molecular, Cellular and Developmental Biology, 266 Whitney Avenue, New Haven, Connecticut 06520, USA
Nat Rev Cancer 6:259-69. 2006b>MicroRNAs (miRNAs) are an abundant class of small non-protein-coding RNAs that function as negative gene regulators...
Origin, biogenesis, and activity of plant microRNAsOlivier Voinnet
Institut de Biologie Moleculaire des Plantes, CNRS UPR2357 Université de Strasbourg, 67084 Strasbourg, France
Cell 136:669-87. 2009b>MicroRNAs (miRNAs) are key posttranscriptional regulators of eukaryotic gene expression. Plants use highly conserved as well as more recently evolved, species-specific miRNAs to control a vast array of biological processes...
The widespread regulation of microRNA biogenesis, function and decayJacek Krol
Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland
Nat Rev Genet 11:597-610. 2010b>MicroRNAs (miRNAs) are a large family of post-transcriptional regulators of gene expression that are approximately 21 nucleotides in length and control many developmental and cellular processes in eukaryotic organisms...
Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIPMarkus Hafner
Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10065, USA
Cell 141:129-41. 2010..The precise mapping of binding sites across the transcriptome will be critical to the interpretation of the rapidly emerging data on genetic variation between individuals and how these variations contribute to complex genetic diseases...
Real-time quantification of microRNAs by stem-loop RT-PCRCaifu Chen
Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA
Nucleic Acids Res 33:e179. 2005..Furthermore, the concept of stem-loop RT primer design could be applied in small RNA cloning and multiplex assays for better specificity and efficiency...
The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1Philip A Gregory
Hanson Institute and Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia
Nat Cell Biol 10:593-601. 2008..Enforced expression of the miR-200 family alone was sufficient to prevent TGF-beta-induced EMT. Together, these microRNAs cooperatively regulate expression of the E-cadherin transcriptional repressors ZEB1 (also known as deltaEF1) and ..
Causes and consequences of microRNA dysregulation in cancerCarlo M Croce
Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University Medical Center, 410 West 10th Avenue, Columbus, OH 43210, USA
Nat Rev Genet 10:704-14. 2009....
The microRNA.org resource: targets and expressionDoron Betel
Computational and Systems Biology Center, Memorial Sloan Kettering Cancer Center, NY, USA
Nucleic Acids Res 36:D149-53. 2008..current biological knowledge on target rules and on the use of an up-to-date compendium of mammalian microRNAs. MicroRNA expression profiles are derived from a comprehensive sequencing project of a large set of mammalian ..
miRBase: integrating microRNA annotation and deep-sequencing dataAna Kozomara
Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
Nucleic Acids Res 39:D152-7. 2011..We have mapped reads from short RNA deep-sequencing experiments to microRNAs in miRBase and developed web interfaces to view these mappings...
The functions of animal microRNAsVictor Ambros
Dartmouth Medical School, Department of Genetics, Hanover, New Hampshire 03755, USA E mail
Nature 431:350-5. 2004b>MicroRNAs (miRNAs) are small RNAs that regulate the expression of complementary messenger RNAs. Hundreds of miRNA genes have been found in diverse animals, and many of these are phylogenetically conserved...
MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancerFanyin Meng
Department of Internal Medicine, Scott and White Clinic, Texas A and M University System Health Science Center College of Medicine, Temple, Texas, USA
Gastroenterology 133:647-58. 2007b>microRNAs (miRNAs) are short noncoding RNAs that regulate gene expression negatively...
A microRNA expression signature of human solid tumors defines cancer gene targetsStefano Volinia
Department of Molecular Virology, Immunology, and Medical Genetics and Cancer Comprehensive Center, Ohio State University, Columbus, OH 43210, USA
Proc Natl Acad Sci U S A 103:2257-61. 2006Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers...
NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responsesKonstantin D Taganov
Division of Biology, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
Proc Natl Acad Sci U S A 103:12481-6. 2006..b>MicroRNAs have been implicated as negative regulators controlling diverse biological processes at the level of ..
Many roads to maturity: microRNA biogenesis pathways and their regulationJulia Winter
Helmholtz University Group Molecular RNA Biology and Cancer, German Cancer Research Center DKFZ and Institute of Pathology, University of Heidelberg, B150 INF 581, D 69120 Heidelberg, Germany
Nat Cell Biol 11:228-34. 2009b>MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as development and cancer...
Small silencing RNAs: an expanding universeMegha Ghildiyal
Department of Biochemistry and Molecular Pharmacology and Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
Nat Rev Genet 10:94-108. 2009..in 1993 of the first small silencing RNA, a dizzying number of small RNA classes have been identified, including microRNAs (miRNAs), small interfering RNAs (siRNAs) and Piwi-interacting RNAs (piRNAs)...
The database of experimentally supported targets: a functional update of TarBaseGiorgos L Papadopoulos
Institute of Molecular Oncology, Biomedical Sciences Research Center Alexander Fleming, 166 72 Varkiza, Synaptic Ltd, 711 10 Heraklion, Greece
Nucleic Acids Res 37:D155-8. 2009..Additionally, the database is functionally linked to several other relevant and useful databases such as Ensembl, Hugo, UCSC and SwissProt. The TarBase5.0 database can be queried or downloaded from http://microrna.gr/tarbase...
Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseasesXi Chen
Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, China
Cell Res 18:997-1006. 2008Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers...
Genome-wide mapping of in vivo protein-DNA interactionsDavid S Johnson
Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305 5120, USA
Science 316:1497-502. 2007..96] and statistical confidence (P <10(-4)), properties that were important for inferring new candidate interactions. These include key transcription factors in the gene network that regulates pancreatic islet cell development...
Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosisTsung Cheng Chang
The McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Mol Cell 26:745-52. 2007..In this report, we demonstrate that microRNAs (miRNAs) are important components of the p53 transcriptional network...
microRNA-directed phasing during trans-acting siRNA biogenesis in plantsEdwards Allen
Center for Gene Research and Biotechnology, Oregon State University, Corvallis, Oregon 97331, USA
Cell 121:207-21. 2005Plants and animals use small RNAs (microRNAs [miRNAs] and siRNAs) as guides for posttranscriptional and epigenetic regulation...
MicroRNA-21 is an antiapoptotic factor in human glioblastoma cellsJennifer A Chan
Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
Cancer Res 65:6029-33. 2005b>MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate protein expression by targeting the mRNA of protein-coding genes for either cleavage or repression of translation...
miRecords: an integrated resource for microRNA-target interactionsFeifei Xiao
Department of Neuroscience, University of Minnesota, Minneapolis, MN 55455, USA
Nucleic Acids Res 37:D105-10. 2009b>MicroRNAs (miRNAs) are an important class of small noncoding RNAs capable of regulating other genes' expression. Much progress has been made in computational target prediction of miRNAs in recent years...
Combinatorial microRNA target predictionsAzra Krek
Center for Comparative Functional Genomics, Department of Biology, New York University, 100 Washington Square East, New York, New York 10003, USA
Nat Genet 37:495-500. 2005b>MicroRNAs are small noncoding RNAs that recognize and bind to partially complementary sites in the 3' untranslated regions of target genes in animals and, by unknown mechanisms, regulate protein production of the target transcript...
Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNACesar Llave
Center for Gene Research and Biotechnology, and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
Science 297:2053-6. 2002....
Criteria for annotation of plant MicroRNAsBlake C Meyers
Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711, USA
Plant Cell 20:3186-90. 2008b>MicroRNAs (miRNAs) are approximately 21 nucleotide noncoding RNAs produced by Dicer-catalyzed excision from stem-loop precursors...
MicroRNAs in CancerRamiro Garzon
Department of Medicine and Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA
Annu Rev Med 60:167-79. 2009b>MicroRNAs (miRNAs) are small, noncoding RNAs with important functions in development, cell differentiation, and regulation of cell cycle and apoptosis...
MicroRNAs in cancer: small molecules with a huge impactMarilena V Iorio
Molecular Biology Unit, Department of Experimental Oncology, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico, Istituto Nazionale Tumori, Milano, Italy
J Clin Oncol 27:5848-56. 2009Every cellular process is likely to be regulated by microRNAs, and an aberrant microRNA expression signature is a hallmark of several diseases, including cancer...
MicroRNA targeting specificity in mammals: determinants beyond seed pairingAndrew Grimson
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Mol Cell 27:91-105. 2007Mammalian microRNAs (miRNAs) pair to 3'UTRs of mRNAs to direct their posttranscriptional repression. Important for target recognition are approximately 7 nt sites that match the seed region of the miRNA...
Widespread changes in protein synthesis induced by microRNAsMatthias Selbach
Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13125 Berlin, Germany
Nature 455:58-63. 2008Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs. It is unknown how much translational control is exerted by miRNAs on a genome-wide scale...
MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3BMuller Fabbri
Department of Molecular Virology, Immunology, and Medical Genetics, Comprehensive Cancer Center, and College of Pharmacy, Ohio State University, Columbus, OH 43210, USA
Proc Natl Acad Sci U S A 104:15805-10. 2007b>MicroRNAs (miRNAs) are small, noncoding RNAs that regulate expression of many genes. Recent studies suggest roles of miRNAs in carcinogenesis. We and others have shown that expression profiles of miRNAs are different in lung cancer vs...
The nuclear RNase III Drosha initiates microRNA processingYoontae Lee
Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
Nature 425:415-9. 2003Hundreds of small RNAs of approximately 22 nucleotides, collectively named microRNAs (miRNAs), have been discovered recently in animals and plants...
MicroRNA control of signal transductionMasafumi Inui
Department of Histology, Microbiology and Medical Biotechnologies, Section of Histology and Embryology, University of Padua, viale Colombo 3, 35100 Padua, Italy
Nat Rev Mol Cell Biol 11:252-63. 2010b>MicroRNAs (miRNAs) are integral elements in the post-transcriptional control of gene expression. After the identification of hundreds of miRNAs, the challenge is now to understand their specific biological function...
A dicer-independent miRNA biogenesis pathway that requires Ago catalysisSihem Cheloufi
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
Nature 465:584-9. 2010..Homozygous mutants died shortly after birth with an obvious anaemia. Examination of microRNAs and their potential targets revealed a loss of miR-451, a small RNA important for erythropoiesis...
A coding-independent function of gene and pseudogene mRNAs regulates tumour biologyLaura Poliseno
Cancer Genetics Program, Beth Israel Deaconess Cancer Center, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
Nature 465:1033-8. 2010..However, given that microRNAs bind to RNAs, we hypothesized that RNAs could possess a regulatory role that relies on their ability to compete ..
MiR-150 controls B cell differentiation by targeting the transcription factor c-MybChangchun Xiao
The CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
Cell 131:146-59. 2007....
Biogenesis of small RNAs in animalsV Narry Kim
School of Biological Sciences and Center for National Creative Research, Seoul National University, Seoul, 151 742, Korea
Nat Rev Mol Cell Biol 10:126-39. 2009..classes based on their biogenesis mechanism and the type of Argonaute protein that they are associated with: microRNAs (miRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and Piwi-interacting RNAs (piRNAs)...
Physiological and pathological roles for microRNAs in the immune systemRyan M O'Connell
Division of Biology, California Institute of Technology, 330 Braun, 1200 East California Boulevard, Pasadena, California 91125, USA
Nat Rev Immunol 10:111-22. 2010Mammalian microRNAs (miRNAs) have recently been identified as important regulators of gene expression, and they function by repressing specific target genes at the post-transcriptional level...
Rice MicroRNA effector complexes and targetsLiang Wu
National Institute of Biological Sciences, Beijing, China
Plant Cell 21:3421-35. 2009b>MicroRNAs (miRNAs) are small silencing RNAs with regulatory roles in gene expression. miRNAs interact with Argonaute (AGO) proteins to form effector complexes that cleave target mRNAs or repress translation...
A microRNA component of the p53 tumour suppressor networkLin He
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nature 447:1130-4. 2007..The p53 network suppresses tumour formation through the coordinated activation of multiple transcriptional targets, and miR-34 may act in concert with other effectors to inhibit inappropriate cell proliferation...
Human MicroRNA targetsBino John
Computational Biology Center, Memorial Sloan-Kettering Cancer Center, New York, New York, USA
PLoS Biol 2:e363. 2004b>MicroRNAs (miRNAs) interact with target mRNAs at specific sites to induce cleavage of the message or inhibit translation. The specific function of most mammalian miRNAs is unknown...
Lost in translation: an assessment and perspective for computational microRNA target identificationPanagiotis Alexiou
Institute of Molecular Oncology, Biomedical Sciences Research Center Alexander Fleming, 166 72 Varkiza, Greece
Bioinformatics 25:3049-55. 2009b>MicroRNAs (miRNAs) are a class of short endogenously expressed RNA molecules that regulate gene expression by binding directly to the messenger RNA of protein coding genes...
A novel class of small RNAs: tRNA-derived RNA fragments (tRFs)Yong Sun Lee
Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908, USA
Genes Dev 23:2639-49. 2009New types of small RNAs distinct from microRNAs (miRNAs) are progressively being discovered in various organisms...
Identification of grapevine microRNAs and their targets using high-throughput sequencing and degradome analysisVitantonio Pantaleo
Istituto di Virologia Vegetale, Consiglio Nazionale delle Ricerche, Torino, Italy
Plant J 62:960-76. 2010In plants, microRNAs (miRNAs) comprise one of three classes of small RNAs regulating gene expression at the post-transcriptional level...
Expanding the microRNA targeting code: functional sites with centered pairingChanseok Shin
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
Mol Cell 38:789-802. 2010..This expanded repertoire of cleavage targets and the identification of the centered site type help explain why central regions of many miRNAs are evolutionarily conserved...
Circulating microRNAs in patients with coronary artery diseaseStephan Fichtlscherer
Institute for Cardiovascular Regeneration, Goethe University Frankfurt, Theodor Stern Kai 7, Frankfurt, Germany
Circ Res 107:677-84. 2010b>MicroRNAs are small RNAs that control gene expression. Besides their cell intrinsic function, recent studies reported that microRNAs are released by cultured cells and can be detected in the blood.
Highly specific gene silencing by artificial microRNAs in ArabidopsisRebecca Schwab
Department of Molecular Biology, Max Planck Institute for Developmental Biology, , Germany
Plant Cell 18:1121-33. 2006Plant microRNAs (miRNAs) affect only a small number of targets with high sequence complementarity, while animal miRNAs usually have hundreds of targets with limited complementarity...
MicroRNAs: new regulators of immune cell development and functionDavid Baltimore
Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
Nat Immunol 9:839-45. 2008..Here we bring together much of this work, which has so far only scratched the surface of this very fertile field of investigation, and show how the results illuminate many historic questions about hematopoiesis and immune function...
MicroRNAs: small RNAs with a big role in gene regulationLin He
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nat Rev Genet 5:522-31. 2004
Intronic microRNA precursors that bypass Drosha processingJ Graham Ruby
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Nature 448:83-6. 2007b>MicroRNAs (miRNAs) are approximately 22-nucleotide endogenous RNAs that often repress the expression of complementary messenger RNAs...
Structural features of small RNA precursors determine Argonaute loading in Caenorhabditis elegansFlorian A Steiner
Hubrecht Institute, Koninklijke Nederlandse Akademie van Wetenschappen, Utrecht, 3584 CT, The Netherlands
Nat Struct Mol Biol 14:927-33. 2007In C. elegans, DCR-1 is required for the maturation of both short interfering RNAs (siRNAs) and microRNAs (miRNAs), which are subsequently loaded into different Argonaute proteins to mediate silencing via distinct mechanisms...
[MiRNA in disease and therapeutic development]Yoichiro Tanaka
Tanpakushitsu Kakusan Koso 51:2509-14. 2006
Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis transcriptomeJulia A Chekanova
School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
Cell 131:1340-53. 2007..These first genome-wide maps of exosome substrates will aid in illuminating new fundamental components and regulatory mechanisms of eukaryotic transcriptomes...
The roles of plant dsRNA-binding proteins in RNAi-like pathwaysShaun J Curtin
CSIRO Plant Industry Canberra, P O Box 1600, ACT 2601, Australia
FEBS Lett 582:2753-60. 2008..DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway...
A silencing safeguard: links between RNA silencing and mRNA processing in ArabidopsisXuemei Chen
Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA 92521, USA
Dev Cell 14:811-2. 2008....
Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formationTaiowa A Montgomery
Molecular and Cellular Biology Program, Oregon State University, Corvallis, OR 97331, USA
Cell 133:128-41. 2008..miR390 and AGO7, therefore, evolved as a highly specific miRNA guide/effector protein pair to function at two distinct tasiRNA biogenesis steps...
Two molecular features contribute to the Argonaute specificity for the microRNA and RNAi pathways in C. elegansGuillaume Jannot
Laval University Cancer Research Centre, Hôtel Dieu de Québec CHUQ, Quebec City, Quebec G1R 2J6, Canada
RNA 14:829-35. 2008..Thus, our data suggest that the selection of Argonaute proteins is affected by two molecular features: (1) the structure of the small RNA duplex; and (2) the Argonautes specific characteristics...
Inhibition of HIV-1 by multiple siRNAs expressed from a single microRNA polycistronYing Poi Liu
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam CINIMA, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
Nucleic Acids Res 36:2811-24. 2008..These combined results indicate that a multiplex miRNA strategy may be a promising therapeutic approach to attack escape-prone viral pathogens...
GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decayAna Eulalio
Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
Nat Struct Mol Biol 15:346-53. 2008b>MicroRNAs (miRNAs) silence gene expression by binding 3' untranslated regions of target mRNAs...
Let me count the ways: mechanisms of gene regulation by miRNAs and siRNAsLigang Wu
Kimmel Center for Biology and Medicine, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
Mol Cell 29:1-7. 2008..Moreover, under certain conditions, miRNAs are also capable of activating translation. A variety of cellular proteins have been implicated in these regulatory mechanisms, yet their exact roles remain largely unresolved...
Hibiscus chlorotic ringspot virus coat protein inhibits trans-acting small interfering RNA biogenesis in ArabidopsisChunying Meng
Department of Biological Sciences, 14 Science Drive 4, National University of Singapore, 117545 Singapore
J Gen Virol 89:2349-58. 2008..The reduced accumulation of ta-siRNA might result from the interference of HCRSV CP with Dicer-like protein(s), responsible for the generation of dsRNA in ta-siRNA biogenesis...
A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtiiTao Zhao
National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
Genes Dev 21:1190-203. 2007..Plants and animals contain many microRNAs (miRNAs) that play vital roles in development, including helping to specify cell type and tissue identity...
RNA interference: methylation mysteryMichael Ronemus
Nature 433:472-3. 2005
MicroRNA-9 controls the expression of Granuphilin/Slp4 and the secretory response of insulin-producing cellsValérie Plaisance
Department of Cell Biology and Morphology, University of Lausanne, and Department of Internal Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
J Biol Chem 281:26932-42. 2006..b>MicroRNAs are newly discovered small non-coding RNAs acting as repressors of gene expression...
Asymmetry in the assembly of the RNAi enzyme complexDianne S Schwarz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, MA 01605, USA
Cell 115:199-208. 2003..Asymmetry is the hallmark of a related class of small, single-stranded, noncoding RNAs, microRNAs (miRNAs)...
La Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNASamantha S Soldan
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 4283, USA
J Virol 79:234-44. 2005..Additionally, NSs will help us to identify molecular pathways involved in RNAi and further define its role in the innate immune system...
Loss of function of OsDCL1 affects microRNA accumulation and causes developmental defects in riceBin Liu
National Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing
Plant Physiol 139:296-305. 2005b>MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are two types of noncoding RNAs involved in developmental regulation, genome maintenance, and defense in eukaryotes...
Ectopic DICER-LIKE1 expression in P1/HC-Pro Arabidopsis rescues phenotypic anomalies but not defects in microRNA and silencing pathwaysSizolwenkosi Mlotshwa
Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, USA
Plant Cell 17:2873-85. 2005..These data suggest that P1/HC-Pro defects in development do not result from general impairments in small RNA pathways and raise the possibility that DCL1 participates in processes in addition to miRNA biogenesis...
[RNA binding proteins in the RNA interference phenomenon]S G Shpiz
Mol Biol (Mosk) 40:595-608. 2006..The interactions of these domains with the earlier well known RNA binding modules in RNAi proteins are described...
Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathwaysYoung Sik Lee
Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
Cell 117:69-81. 2004..However, Dicer-1 but not Dicer-2 is essential for miRISC-directed translation repression. Thus, siRISCs and miRISCs are different with respect to Dicers in Drosophila...
mRNA turnover meets RNA interferenceDmitry Belostotsky
Department of Biological Sciences, State University of New York at Albany, Albany, New York 12222, USA
Mol Cell 16:498-500. 2004..By using two very different approaches, recent work by Gazzani et al. (2004) and Souret et al. (2004) reveal a fundamental link between mRNA degradation and RNA silencing pathways in Arabidopsis...
Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAsFranck Vazquez
, UPRES-EA3569, IFR118, EPT1016, , , , 59650 Villeneuve d'Ascq Cedex, France
Mol Cell 16:69-79. 2004..The identification of this silencing pathway provides yet another dimension to posttranscriptional mRNA regulation in plants...
A two-hit trigger for siRNA biogenesis in plantsMichael J Axtell
Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
Cell 127:565-77. 2006..Therefore, two "hits,"--often, but not always, two cleavage events--constitute a conserved trigger for siRNA biogenesis, a finding with implications for recognition and silencing of aberrant RNA...
Probing the microRNA and small interfering RNA pathways with virus-encoded suppressors of RNA silencingPatrice Dunoyer
, 67084 Strasbourg Cedex, France
Plant Cell 16:1235-50. 2004In plants, small interfering RNAs (siRNAs) and microRNAs (miRNAs) are effectors of RNA silencing, a process involved in defense through RNA interference (RNAi) and in development...
Small RNAs: the genome's guiding hand?Carina Dennis
Nature 420:732. 2002
siRNAs can function as miRNAsJohn G Doench
Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Genes Dev 17:438-42. 2003..partially complementary binding sites in its 3' UTR, much like the demonstrated function of endogenously encoded microRNAs (miRNAs)...
Evidence for nuclear processing of plant micro RNA and short interfering RNA precursorsIstvan Papp
Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A 5020 Salzburg, Austria
Plant Physiol 132:1382-90. 2003..The results are consistent with a model in which dsRNA precursors of miRNAs and at least some 21- to 22-nt siRNAs are processed in the nucleus, the former by nuclear DCL1 and the latter by an unknown nuclear DCL...
Sequence-specific interference by small RNAs derived from adenovirus VAI RNAMasayuki Sano
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST, Central 4, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan
FEBS Lett 580:1553-64. 2006..mapping and further confirmed that the processed small RNA can act as small interfering RNAs (siRNAs) or as microRNAs (miRNAs) in transient transfection assays and during viral infection...
A biochemical framework for RNA silencing in plantsGuiliang Tang
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 17:49-63. 2003..Thus, endonuclease complexes guided by small RNAs are a common feature of RNA silencing in both animals and plants...
A novel method to detect functional microRNA targetsSergei Vatolin
Brain Tumor Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
J Mol Biol 358:983-96. 2006....
MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in ArabidopsisMichael Ronemus
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
Plant Cell 18:1559-74. 2006..These results indicate that a subset of endogenous mRNA targets of RNA interference may be regulated through a mechanism of second-strand RNA synthesis and degradation initiated by or in addition to miRNA-mediated cleavage...
Plant science. Viruses face a double defense by plant small RNAsPeter M Waterhouse
CSIRO Plant Industry, Canberra, ACT 2601, Australia
Science 313:54-5. 2006
Human RISC couples microRNA biogenesis and posttranscriptional gene silencingRichard I Gregory
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
Cell 123:631-40. 2005..Importantly, ATP is not required for miRNA processing, RISC assembly, or multiple rounds of target-RNA cleavage. These results define the composition of RISC and demonstrate that miRNA processing and target-RNA cleavage are coupled...
High impactAleel K Grennan
University of Illinois Urbana, IL, USA
Plant Physiol 139:563-5. 2005
Breakthrough of the year. Small RNAs make big splashJennifer Couzin
Science 298:2296-7. 2002
Small RNA asymmetry in RNAi: function in RISC assembly and gene regulationGyorgy Hutvagner
University of Dundee, School of Life Sciences, Division of Gene Regulation and Expression, Dundee DD1 4HN, UK
FEBS Lett 579:5850-7. 2005..Here, I review the rules of the asymmetric RISC formation and discuss their possible regulatory function in several steps in RNAi...
Approaches for chemically synthesized siRNA and vector-mediated RNAiMohammed Amarzguioui
Division of Molecular Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010-3011, USA
FEBS Lett 579:5974-81. 2005....
Viral virulence protein suppresses RNA silencing-mediated defense but upregulates the role of microrna in host gene expressionJun Chen
Institute of Molecular and Cell Biology, National University of Singapore, Singapore 117609
Plant Cell 16:1302-13. 2004Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are processed by the ribonuclease Dicer from distinct precursors, double-stranded RNA (dsRNA) and hairpin RNAs, respectively, although either may guide RNA silencing via a similar ..
Research Grants
- Micro RNA Expression and CancerThomas Schmittgen; Fiscal Year: 2005..Assays developed with this research may help to identify the function of a new class of genes in humans. ..
- microRNA Uncoupling of Protein and Transcript Expression in Liver RegenerationClifford Steer; Fiscal Year: 2009..b>MicroRNAs are recently discovered ~ 22 nt non-coding RNAs that are known to be critical effectors of gene regulation via ..
- ROLE OF MINERALOCORTICOIDS IN HYPERTENSIONCelso Gomez Sanchez; Fiscal Year: 2009..b>MicroRNAs are a class of regulators of gene expression that have recently been found to be crucial for pancreatic islet ..
- Role of noncoding RNAs in schizophreniaClaes Wahlestedt; Fiscal Year: 2009..The goals of this proposal are threefold: First, we will investigate expression patterns of brain-specific microRNAs (miRNAs) in NMDA-R- related animal models of schizophrenia...
- Role of miRNAs in Vascular PhysiologyAkiko Hata; Fiscal Year: 2009..MiR-21 belongs to a family of short, noncoding, single-stranded RNAs (ssRNAs) called microRNAs (miRNAs) that regulate gene expression by targeting mRNAs in a sequence-specific manner, causing translational ..
- Role of Brain-Specific microRNAs in Suppression of Autoimmune NeuroinflammationEugene D Ponomarev; Fiscal Year: 2010b>MicroRNAs are a growing family of conserved molecules involved in normal biological processes including cell growth and differentiation and pathological conditions such as cancer...
- ROLE OF MINERALOCORTICOIDS IN HYPERTENSIONCELSO ENRIQUE GOMEZ SANCHEZ; Fiscal Year: 2010..b>MicroRNAs are a class of regulators of gene expression that have recently been found to be crucial for pancreatic islet ..
- microRNA Uncoupling of Protein and Transcript Expression in Liver RegenerationCLIFFORD JOHN STEER; Fiscal Year: 2010..b>MicroRNAs are recently discovered ~ 22 nt non-coding RNAs that are known to be critical effectors of gene regulation via ..
- Role of noncoding RNAs in schizophreniaCLAES ROBERT contact WAHLESTEDT; Fiscal Year: 2010..The goals of this proposal are threefold: First, we will investigate expression patterns of brain-specific microRNAs (miRNAs) in NMDA-R- related animal models of schizophrenia...
- MicroRNAs and chromosome 22q in the colonAnil K Rustgi; Fiscal Year: 2010..We now have discovered a new telomeric region of LOH, which harbors two microRNAs, namely Let-7a3 and Let-7b, and these microRNAs are downregulated in up to 40% of colorectal cancers...
- MicroRNAs and chromosome 22q in the colonAnil Rustgi; Fiscal Year: 2009..We now have discovered a new telomeric region of LOH, which harbors two microRNAs, namely Let-7a3 and Let-7b, and these microRNAs are downregulated in up to 40% of colorectal cancers...
- MIcroRNA: A Novel Genetic Modifier for Breast Cancer PredispositionHua Zhao; Fiscal Year: 2010The discovery of microRNAs (miRNAs) in the last decade, and the realization of their growing importance in carcinogenesis and cancer prognosis through regulation of transcription of oncogenes and tumor suppressor genes (TSGs), has led ..
- Suppression of MMP-13 Expression in Arthritis by PomegranateTariq M Haqqi; Fiscal Year: 2010..Currently the only effective treatment is surgical joint replacement. MicroRNAs (miRNA) are a class of non-coding RNAs regulating gene expression by sequence specific inhibition of target mRNA ..
- THE THERAPY OF AMLMichael Andreeff; Fiscal Year: 2007..The new program has two major additions: Dr. C. Croce and his group has joined us in studying the role of microRNAs in AML and already identified miR's that target Bcl-2, Mcl-1 and KIT and Drs. G. Mills and S...
- Understanding microRNA Biogenesis and FunctionPhillip Zamore; Fiscal Year: 2009b>MicroRNAs (miRNAs), ~22 nt long, single-stranded RNAs, guide protein complexes to block expression of mRNAs to which they bind by base pairing. The first microRNA was discovered in 1993; the second, in 2000...
- Understanding microRNA Biogenesis and FunctionPhillip D Zamore; Fiscal Year: 2010b>MicroRNAs (miRNAs), ~22 nt long, single-stranded RNAs, guide protein complexes to block expression of mRNAs to which they bind by base pairing. The first microRNA was discovered in 1993;the second, in 2000...
- Genomic targets of oncoproteins and tumor suppressorsKevin Struhl; Fiscal Year: 2010..e. the ordered series of steps involving transcription factors, direct target genes, microRNAs, and microRNA targets) by which an inflammatory signal leads to cellular transformation...
- Understanding microRNA Biogenesis and FunctionPhillip Zamore; Fiscal Year: 2007b>MicroRNAs (miRNAs), ~22 nt long, single-stranded RNAs, guide protein complexes to block expression of mRNAs to which they bind by base pairing. The first microRNA was discovered in 1993; the second, in 2000...
- Understanding microRNA Biogenesis and FunctionPhillip Zamore; Fiscal Year: 2007b>MicroRNAs (miRNAs), ~22 nt long, single-stranded RNAs, guide protein complexes to block expression of mRNAs to which they bind by base pairing. The first microRNA was discovered in 1993; the second, in 2000...
- Efficient Labeling of MicroRNA for Microarray AnalysisMary Anne Watt; Fiscal Year: 20076. Project Summary/Abstract MicroRNAs (miRNAs) are a group of small, non-coding RNAs, approximately 19-23 nucleotides in length, that regulate gene expression by binding to sequences within the 3' untranslated regions of specific target ..
- Role of "Local" MicroRNAs and CGG Repeat-Derived RNAs in Fragile X SyndromeSAMIE JAFFREY; Fiscal Year: 2009The objective of the proposed research is to identify roles for microRNAs and other small RNAs in the etiology of mental retardation and fragile X syndrome (FXS)...
- Role of "Local" MicroRNAs and CGG Repeat-Derived RNAs in Fragile X SyndromeSamie R Jaffrey; Fiscal Year: 2010The objective of the proposed research is to identify roles for microRNAs and other small RNAs in the etiology of mental retardation and fragile X syndrome (FXS)...
- Role of "Local" MicroRNAs and CGG Repeat-Derived RNAs in Fragile X SyndromeSAMIE JAFFREY; Fiscal Year: 2007The objective of the proposed research is to identify roles for microRNAs and other small RNAs in the etiology of mental retardation and fragile X syndrome (FXS)...
- MicroRNA regulation of human T and B cell activationDaniel R Salomon; Fiscal Year: 2010..One class of ncRNAs are microRNAs (miRNAs), discovered in 1993, that are believed to regulate almost 30% of all human genes...
- MicroRNAs and chromosome 22q in the colonAnil Rustgi; Fiscal Year: 2009..We now have discovered a new telomeric region of LOH, which harbors two microRNAs, namely Let-7a3 and Let-7b, and these microRNAs are downregulated in up to 40% of colorectal cancers...
- microRNA expression profiling of benign breast tissue and breast cancer riskThomas E Rohan; Fiscal Year: 2010b>MicroRNAs (miRNAs) are endogenous, small non-coding RNAs that control gene expression by directing their target mRNAs for degradation and/or posttranscriptional repression...
- Nuclear Function of Abl in DNA Damage ResponseJean Wang; Fiscal Year: 2009..We have recently found that activation of Abl tyrosine kinase can regulate the expression of microRNAs. MicroRNAs are non-coding RNAs that are between 18~25 nucleotides in length...
- Stress and proliferation states impact microRNA-mediated regulation in cancerPHILLIP SHARP; Fiscal Year: 2009Changes in the levels of microRNAs and effects of over-expression of microRNAs have already been related to human malignancy...
- Elucidating the functions of microRNA in skin development and skin stem cellsRui Yi; Fiscal Year: 2007..b>MicroRNAs are a family of non-coding small RNAs conserved from Caenorhabditis elegans to human...
- Vestibulo-cerebellar contribution to spatial adaptationNEAL BARMACK; Fiscal Year: 2009..b>MicroRNAs regulate aspects of developmental plasticity and apoptosis...
- MicroRNAs in Human Ovarian CancerJin Q Cheng; Fiscal Year: 2010..b>MicroRNAs (miRNA) are a class of small RNAs that are phylogenetically conserved and play important roles in the regulation ..
- MicroRNAs in Human Ovarian CancerJin Cheng; Fiscal Year: 2009..b>MicroRNAs (miRNA) are a class of small RNAs that are phylogenetically conserved and play important roles in the regulation ..
- Regulation of miRNA in breast cancerCarolyn M Klinge; Fiscal Year: 2010..b>MicroRNAs (miRNAs) are a class of naturally-occurring, small, non-coding RNA molecules that are involved in regulating the ..
- A Plasmid-Based miRNA Sensor Library for Use in MiceDavid Lewis; Fiscal Year: 2006Project Summary/Abstract: MicroRNAs (miRNAs) are small, nuclear encoded, single-stranded RNAs involved in regulating gene expression...
- MicroRNA Cellular Functions and Target Identification in Acute Myeloid LeukemiaElizabeth Mambo; Fiscal Year: 2006..The recently discovered gene regulatory RNAs known as microRNAs (miRNAs) have been implicated in various types of cancer including lung, breast and chronic lymphocytic leukemia ..
- Gene expression analysis of coding and non-coding RNAs in colon cancer preventionROBERT CHAPKIN; Fiscal Year: 2007..Unfortunately, to date, the effect of chemopreventive agents on microRNAs and their messenger RNA (mRNA) targets during different stages of colon cancer has not been determined...
- MicroRNA Gene Expression Mapping in Chicken EmbryosPARKER ANTIN; Fiscal Year: 2007..of this competing supplemental application is to map by whole mount in situ hybridization the expression of all microRNAs in the chicken embryo through day 8 of development, and to optimize technologies for detecting mRNAs in vivo ..
- Functions of Dicer, Drosha and miRNAs in the skinSarah Millar; Fiscal Year: 2009..The recent discovery of post-transcriptional regulation by microRNAs (miRNAs) revealed a previously unsuspected mechanism by which gene expression is controlled and refined...
- The Role of MicroRNAs in Corneal Epithelial HomeostasisRobert M Lavker; Fiscal Year: 2010The long term goal of this project is to understand how microRNAs (miRNAs) function in regulating the corneal and limbal epithelia...
- Functions of Dicer, Drosha and miRNAs in the skinSarah Millar; Fiscal Year: 2007..The recent discovery of post-transcriptional regulation by microRNAs (miRNAs) revealed a previously unsuspected mechanism by which gene expression is controlled and refined...
