phosphoserine

Summary

Summary: The phosphoric acid ester of serine.

Top Publications

  1. ncbi Formation of D-serine from L-phosphoserine in brain synaptosomes
    P L Wood
    CoCensys, Inc, Irvine, California 92718, USA
    J Neurochem 67:1485-90. 1996
  2. ncbi Molecular plasticity in the rat auditory brainstem: modulation of expression and distribution of phosphoserine, phospho-CREB and TrkB after noise trauma
    Steffen A Michler
    Neurobiological Research Laboratory, Department of Otorhinolaryngology, University of Freiburg, Freiburg, Germany
    Audiol Neurootol 8:190-206. 2003
  3. pmc A Smad action turnover switch operated by WW domain readers of a phosphoserine code
    Eric Aragon
    Structural and Computational Biology Programme, Institute for Research in Biomedicine, Barcelona, Spain
    Genes Dev 25:1275-88. 2011
  4. ncbi Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling
    J W Wu
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USA
    Mol Cell 8:1277-89. 2001
  5. ncbi The structural basis for 14-3-3:phosphopeptide binding specificity
    M B Yaffe
    Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Cell 91:961-71. 1997
  6. doi Expanding the genetic code of Escherichia coli with phosphoserine
    Hee Sung Park
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Science 333:1151-4. 2011
  7. pmc Aging reveals a role for nigral tyrosine hydroxylase ser31 phosphorylation in locomotor activity generation
    Michael F Salvatore
    Department of Pharmacology, Toxicology, and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA
    PLoS ONE 4:e8466. 2009
  8. pmc RIM1alpha phosphorylation at serine-413 by protein kinase A is not required for presynaptic long-term plasticity or learning
    Pascal S Kaeser
    Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 105:14680-5. 2008
  9. pmc CDK targets Sae2 to control DNA-end resection and homologous recombination
    Pablo Huertas
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 455:689-92. 2008
  10. ncbi ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity
    Elisa Oricchio
    Institute of Biology and Molecular Pathology, National Research Council, Rome, Italy
    Cell Cycle 5:88-92. 2006

Detail Information

Publications211 found, 100 shown here

  1. ncbi Formation of D-serine from L-phosphoserine in brain synaptosomes
    P L Wood
    CoCensys, Inc, Irvine, California 92718, USA
    J Neurochem 67:1485-90. 1996
    ..The rate-limiting step in the synthesis of serine is the hydrolysis of phosphoserine by phosphoserine phosphatase (EC 3.1.3.3)...
  2. ncbi Molecular plasticity in the rat auditory brainstem: modulation of expression and distribution of phosphoserine, phospho-CREB and TrkB after noise trauma
    Steffen A Michler
    Neurobiological Research Laboratory, Department of Otorhinolaryngology, University of Freiburg, Freiburg, Germany
    Audiol Neurootol 8:190-206. 2003
    ..for tyrosine receptor kinase B in the ipsilateral VCN between day 3 and day 21 after trauma and an increase in phosphoserine immunostaining in the neuropil of the ipsilateral VCN and in neurons of the contralateral lateral superior ..
  3. pmc A Smad action turnover switch operated by WW domain readers of a phosphoserine code
    Eric Aragon
    Structural and Computational Biology Programme, Institute for Research in Biomedicine, Barcelona, Spain
    Genes Dev 25:1275-88. 2011
    ..Thus, a Smad phosphoserine code and a set of WW domain code readers provide an efficient solution to the problem of coupling TGF-β ..
  4. ncbi Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling
    J W Wu
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USA
    Mol Cell 8:1277-89. 2001
    ..8 A resolution, reveals the formation of a homotrimer mediated by the C-terminal phosphoserine (pSer) residues...
  5. ncbi The structural basis for 14-3-3:phosphopeptide binding specificity
    M B Yaffe
    Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Cell 91:961-71. 1997
    The 14-3-3 family of proteins mediates signal transduction by binding to phosphoserine-containing proteins...
  6. doi Expanding the genetic code of Escherichia coli with phosphoserine
    Hee Sung Park
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Science 333:1151-4. 2011
    O-Phosphoserine (Sep), the most abundant phosphoamino acid in the eukaryotic phosphoproteome, is not encoded in the genetic code, but synthesized posttranslationally...
  7. pmc Aging reveals a role for nigral tyrosine hydroxylase ser31 phosphorylation in locomotor activity generation
    Michael F Salvatore
    Department of Pharmacology, Toxicology, and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA
    PLoS ONE 4:e8466. 2009
    ....
  8. pmc RIM1alpha phosphorylation at serine-413 by protein kinase A is not required for presynaptic long-term plasticity or learning
    Pascal S Kaeser
    Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 105:14680-5. 2008
    ....
  9. pmc CDK targets Sae2 to control DNA-end resection and homologous recombination
    Pablo Huertas
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 455:689-92. 2008
    ..These findings therefore provide a mechanistic basis for cell-cycle control of DSB repair and highlight the importance of regulating DSB resection...
  10. ncbi ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity
    Elisa Oricchio
    Institute of Biology and Molecular Pathology, National Research Council, Rome, Italy
    Cell Cycle 5:88-92. 2006
    ....
  11. ncbi Cyclic AMP-dependent protein kinase phosphorylation of Drp1 regulates its GTPase activity and mitochondrial morphology
    Chuang Rung Chang
    Cellular Neurology Unit, NINDS, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 282:21583-7. 2007
    ..Thus, protein phosphorylation at Ser(637) results in clear alterations in Drp1 function and mitochondrial morphology that are likely involved in dynamic regulation of mitochondrial division in cells...
  12. ncbi MDM2-regulated degradation of HIPK2 prevents p53Ser46 phosphorylation and DNA damage-induced apoptosis
    Cinzia Rinaldo
    Department of Experimental Oncology, Regina Elena Cancer Institute, Via delle Messi d Oro 156, 00158 Rome, Italy
    Mol Cell 25:739-50. 2007
    ....
  13. ncbi Crystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition
    R Dajani
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, United Kingdom
    Cell 105:721-32. 2001
    ....
  14. pmc Phosphorylation of Plk1 at Ser326 regulates its functions during mitotic progression
    J Tang
    Department of Biochemistry, Cancer Center, Purdue University, West Lafayette, IN 47907, USA
    Oncogene 27:6635-45. 2008
    ..Only Plk1-S326E, but not the Plk1-S326A, efficiently rescued the p38 or MK2-depletion-induced mitotic defects, further solidifying the requirement of S326 phosphorylation during mitotic progression...
  15. pmc The 14-3-3tau phosphoserine-binding protein is required for cardiomyocyte survival
    Jeffrey M C Lau
    Center for Cardiovascular Research, Washington University School of Medicine, 660 South Euclid Ave, St Louis, MO 63110, USA
    Mol Cell Biol 27:1455-66. 2007
    14-3-3 family members are intracellular dimeric phosphoserine-binding proteins that regulate signal transduction, cell cycle, apoptotic, and metabolic cascades...
  16. pmc ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling
    Thomas Stiff
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, East Sussex, UK
    EMBO J 25:5775-82. 2006
    ..Our findings provide insight into the interplay between the PIKK damage response pathways...
  17. ncbi Phosphoserine-dependent regulation of protein-protein interactions in the Smad pathway
    Jeffrey L Wrana
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Structure 10:5-7. 2002
    ..The activity of this pathway is tightly controlled by serine phosphorylation, which plays a key role in regulating protein-protein interactions that are critical in the elaboration of transcriptional responses...
  18. ncbi Hyaluronan-dependent cell migration can be blocked by a CD44 cytoplasmic domain peptide containing a phosphoserine at position 325
    D Peck
    Department of Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, UK
    J Cell Sci 111:1595-601. 1998
    ..Peptides containing a phosphoserine at residue 325 are efficient blockers of CD44-mediated cell migration but do not reduce CD44 expression or its ..
  19. doi Mechanistic insights into the multistage gas-phase fragmentation behavior of phosphoserine- and phosphothreonine-containing peptides
    Amanda M Palumbo
    Department of Chemistry Michigan State University, East Lansing, Michigan 48824, USA
    J Proteome Res 7:771-9. 2008
    ..examined the multistage collision-induced dissociation (CID) behavior of a set of singly and doubly charged phosphoserine- and phosphothreonine-containing peptide ions, as well as their regioselectively or uniformly deuterated ..
  20. doi Modelling of the gas-phase phosphate group loss and rearrangement in phosphorylated peptides
    Marko Rozman
    Laboratory for Chemical Kinetics and Atmospheric Chemistry, Ruđer Bošković Institute, Bijenicka 54, HR 10002, Zagreb, Croatia
    J Mass Spectrom 46:949-55. 2011
    ..The fragmentation dynamics of phosphoserine versus phosphothreonine containing peptides suggests that H(3)PO(4) neutral loss from phosphothreonine ..
  21. ncbi Effect of modification of hydroxyapatite/collagen composites with sodium citrate, phosphoserine, phosphoserine/RGD-peptide and calcium carbonate on bone remodelling
    Wolfgang Schneiders
    Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus, Fetscherstrasse 74, 01307 Dresden, Germany
    Bone 40:1048-59. 2007
    ..containing type I collagen (HA/Coll) and its modifications with sodium citrate (CI), calcium carbonate (CA), phosphoserine (P) and phosphoserine plus RGD-peptide (RGD)...
  22. pmc AMPK directly inhibits NDPK through a phosphoserine switch to maintain cellular homeostasis
    Rob U Onyenwoke
    Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Mol Biol Cell 23:381-9. 2012
    ....
  23. pmc Interrogation of MDM2 phosphorylation in p53 activation using native chemical ligation: the functional role of Ser17 phosphorylation in MDM2 reexamined
    Changyou Zhan
    Institute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Am Chem Soc 134:6855-64. 2012
    ..Our findings demonstrate that Ser17 phosphorylation is functionally neutral with respect to p53 binding, suggesting that MDM2 phosphorylation at a single site is unlikely to play a dominant role in stress-induced p53 activation...
  24. pmc Dual recognition of phosphoserine and phosphotyrosine in histone variant H2A.X by DNA damage response protein MCPH1
    Namit Singh
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
    Proc Natl Acad Sci U S A 109:14381-6. 2012
    ..X phosphorylation marks. We show that MCPH1 recruitment to sites of DNA damage is linked to both states of H2A.X...
  25. ncbi Sequences of sixteen phosphoserine peptides from ovalbumins of eight species
    J Y Henderson
    Eur J Biochem 114:439-50. 1981
    b>Phosphoserine peptides have been isolated by a diagonal electrophoresis method from proteolytic digests of ovalbumins from hen, grouse, turkey, golden pheasant, magpie goose, chinese goose, Aylesbury duck and fulvous whistling duck...
  26. ncbi Inhibition of the Bcr-Abl oncoprotein by Bcr requires phosphoserine 354
    Natalyn Hawk
    Department of Molecular Pathology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer Res 62:386-90. 2002
    ..The autokinase product contained a M(r) 45,000-47,000 and 55,000 protein. Both species were detected by a Bcr phosphoserine 354 sequence-specific antibody...
  27. doi NMR spectroscopy of 14-3-3ζ reveals a flexible C-terminal extension: differentiation of the chaperone and phosphoserine-binding activities of 14-3-3ζ
    Danielle M Williams
    School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia
    Biochem J 437:493-503. 2011
    Intracellular 14-3-3 proteins bind to many proteins, via a specific phosphoserine motif, regulating diverse cellular tasks including cell signalling and disease progression...
  28. ncbi Phosphorylation of bovine adrenal chromaffin cell tyrosine hydroxylase. Temporal correlation of acetylcholine's effect on site phosphorylation, enzyme activation, and catecholamine synthesis
    J C Waymire
    Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225
    J Biol Chem 263:12439-47. 1988
    ....
  29. ncbi Quantitative and selective fluorophore labeling of phosphoserine on peptides and proteins: characterization at the attomole level by capillary electrophoresis and laser-induced fluorescence
    P Fadden
    Department of Pharmacology, University of Virginia, Charlottesville 22908, USA
    Anal Biochem 225:81-8. 1995
    Reaction conditions were defined for the selective quantitative derivatization and fluorophore labeling of phosphoserine residues on peptides and proteins...
  30. pmc HECT domain-containing E3 ubiquitin ligase Nedd4 interacts with and ubiquitinates Sprouty2
    Francis Edwin
    Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois 60153, USA
    J Biol Chem 285:255-64. 2010
    ..This is the first report demonstrating the regulation of cellular Spry content and its ability to modulate receptor tyrosine kinase signaling by a HECT domain-containing E3 ubiquitin ligase...
  31. pmc Involvement of protein kinase C and Src tyrosine kinase in acute tolerance to ethanol inhibition of spinal NMDA-induced pressor responses in rats
    W K Hsieh
    Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan
    Br J Pharmacol 158:806-18. 2009
    ....
  32. ncbi Function of WW domains as phosphoserine- or phosphothreonine-binding modules
    P J Lu
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Science 283:1325-8. 1999
    ..Although phosphotyrosine-binding modules have been well-characterized, phosphoserine- or phosphothreonine-binding modules have not been described...
  33. ncbi A p53 amino-terminal nuclear export signal inhibited by DNA damage-induced phosphorylation
    Y Zhang
    Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, and Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, NC 27599 7295, USA
    Science 292:1910-5. 2001
    ..Serine-15-phosphorylated p53 induced by ultraviolet irradiation was not exported. Thus, DNA damage-induced phosphorylation may achieve optimal p53 activation by inhibiting both MDM2 binding to, and the nuclear export of, p53...
  34. ncbi KAP1, a novel substrate for PIKK family members, colocalizes with numerous damage response factors at DNA lesions
    David E White
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 66:11594-9. 2006
    ..This discovery provides a novel link between chromatin-mediated transcriptional repression and the recognition/repair of DNA, which must be accomplished by the cellular DNA damage response...
  35. pmc Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells
    Tae Hong Kang
    Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang 790 784, Republic of Korea
    Mol Cell Biol 27:8533-46. 2007
    ..Overexpression of VRK1 resulted in a dramatic condensation of nuclei. Our findings collectively support a role of VRK1 as a novel mitotic histone H3 kinase in mammals...
  36. pmc Androgen receptor phosphorylation and stabilization in prostate cancer by cyclin-dependent kinase 1
    Shaoyong Chen
    Hematology Oncology Division, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 103:15969-74. 2006
    ....
  37. ncbi DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage
    Naoe Taira
    Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, Yushima 1 5 45, Bunkyo ku, Tokyo 113 8510, Japan
    Mol Cell 25:725-38. 2007
    ..Consistent with these results, DYRK2 induces p53AIP1 expression and apoptosis in a Ser46 phosphorylation-dependent manner. These findings indicate that DYRK2 regulates p53 to induce apoptosis in response to DNA damage...
  38. doi Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys)
    Chun Mei Zhang
    Department of Biochemistry and Molecular Biology, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
    Nat Struct Mol Biol 15:507-14. 2008
    ..Cys-tRNA(Cys) by an indirect pathway, whereby O-phosphoseryl-tRNA synthetase (SepRS) acylates tRNA(Cys) with phosphoserine (Sep), and Sep-tRNA-Cys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine...
  39. ncbi Inhibition of myosin light chain kinase by p21-activated kinase
    L C Sanders
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 283:2083-5. 1999
    ..These data indicate that MLCK is a target for PAKs and that PAKs may regulate cytoskeletal dynamics by decreasing MLCK activity and MLC phosphorylation...
  40. ncbi Glycogen synthase kinase-3 beta is a dual specificity kinase differentially regulated by tyrosine and serine/threonine phosphorylation
    Q M Wang
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202 5122
    J Biol Chem 269:14566-74. 1994
    ..Phosphorylation at different residues differentially controls enzyme activity, Ser/Thr phosphorylation causing inactivation and Tyr phosphorylation resulting in increased activity...
  41. ncbi The phosphoserine-585-dependent pathway of the GM-CSF/IL-3/IL-5 receptors mediates hematopoietic cell survival through activation of NF-kappaB and induction of bcl-2
    Mark A Guthridge
    Cytokine Receptor Laboratory, Department of Human Immunology, Institute of Medical and Veterinary Science, Frome Rd, Adelaide, South Australia, Australia 5000
    Blood 103:820-7. 2004
    ..These results demonstrate that both serine and tyrosine phosphorylation pathways play a role in hematopoietic cell survival, are initially independent of each other, and converge on NF-kappaB to promote bcl-2 expression...
  42. ncbi Reduced activation of phosphatidylinositol-3 kinase and increased serine 636 phosphorylation of insulin receptor substrate-1 in primary culture of skeletal muscle cells from patients with type 2 diabetes
    Karim Bouzakri
    INSERM U449 and CRNHL, IFR 62, R Laennec Medical Faculty, F 69370 Lyon Cedex 08, France
    Diabetes 52:1319-25. 2003
    ..Moreover, increased MAPK activity seems to play a role in the phosphorylation of IRS-1 on serine residue in human muscle cells...
  43. ncbi Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1
    B Chu
    Dana Farber Cancer Institute and Joint Center for Radiation Therapy, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 271:30847-57. 1996
    ....
  44. pmc Stt7-dependent phosphorylation during state transitions in the green alga Chlamydomonas reinhardtii
    Sylvain Lemeille
    Department of Molecular Biology and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
    Mol Cell Proteomics 9:1281-95. 2010
    ..Moreover, we also identified phosphorylation sites that are redox (state 2)-dependent but independent of Stt7 and additional phosphorylation sites that are redox-independent...
  45. ncbi BRCT repeats as phosphopeptide-binding modules involved in protein targeting
    Isaac A Manke
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 302:636-9. 2003
    ..BRCA1 carboxyl-terminal) domains in PTIP (Pax transactivation domain-interacting protein) and in BRCA1 as phosphoserine- or phosphothreonine-specific binding modules that recognize substrates phosphorylated by the kinases ATM (..
  46. ncbi PhosphoSerine/threonine binding domains: you can't pSERious?
    M B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Structure 9:R33-8. 2001
    ..Here, we review the insights into serine/threonine phosphorylation-dependent signal transduction processes provided by structures of several of these proteins and their complexes...
  47. doi Isoform-specific defects of insulin stimulation of Akt/protein kinase B (PKB) in skeletal muscle cells from type 2 diabetic patients
    D Cozzone
    INSERM, U 870, IFR62, Faculté de médecine Lyon Sud, Chemin du Grand Revoyet, F 69600 Oullins, France
    Diabetologia 51:512-21. 2008
    ..Because three Akt isoforms exist, each having a distinct physiological role, we investigated the contribution of isoform-specific defects to insulin signalling in human muscle...
  48. ncbi Phosphoserine--a convenient compound for modification of calcium phosphate bone cement collagen composites
    A Reinstorf
    Dresden University of Technology, Department of Materials Science, Max Bergmann Center of Biomaterials, Budapester Str 27, 01062 Dresden, Germany
    J Mater Sci Mater Med 15:451-5. 2004
    ..Furthermore, the proliferation and differentiation were found to be enhanced on the PS-modified material...
  49. ncbi Critical role of WW domain phosphorylation in regulating phosphoserine binding activity and Pin1 function
    Pei Jung Lu
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 277:2381-4. 2002
    b>Phosphoserine-binding modules help determine the specificity of signal transduction events...
  50. ncbi Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding
    XiaoZhe Wang
    Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    J Biol Chem 281:782-91. 2006
    ..These experiments indicate a novel mechanism for the regulation of HSF1 by proinflammatory signaling and may permit HSF1 to respond rapidly to extracellular events, permitting optimal physiological regulation...
  51. ncbi 14-3-3 (epsilon) interacts with the insulin-like growth factor I receptor and insulin receptor substrate I in a phosphoserine-dependent manner
    A Craparo
    Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 272:11663-9. 1997
    ..We present evidence to suggest that 14-3-3 interacts with phosphoserine residues within the COOH terminus of the IGFIR...
  52. ncbi Fluorescent caged phosphoserine peptides as probes to investigate phosphorylation-dependent protein associations
    M Eugenio Vazquez
    Department of Chemistry and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 125:10150-1. 2003
    ..At the same time, the newly developed photolabile 1-(2-nitrophenyl)ethyl-caged phosphoserine allows control of the release of the biologically active ligand through unmasking of the key phosphoserine ..
  53. ncbi Cutting Edge: Internalization of transduced E-selectin by cultured human endothelial cells: comparison of dermal microvascular and umbilical vein cells and identification of a phosphoserine-type di-leucine motif
    Martin S Kluger
    Interdepartmental Program in Vascular Biology and Transplantation, Department of Dermatology, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    J Immunol 168:2091-5. 2002
    ..Control of E-selectin surface expression appears to be phosphoserine dependent, since alanine but not aspartic acid substitution for S581 slows E-selectin internalization.
  54. ncbi Regulation of plant symbiosis receptor kinase through serine and threonine phosphorylation
    Satoko Yoshida
    Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH, United Kingdom
    J Biol Chem 280:9203-9. 2005
    ..Despite the distinct domain architecture of receptor-like kinases versus Pelle/interleukin-1 receptor associated kinase, our results suggest the existence of conserved activation mechanisms...
  55. pmc Melatonin synthesis: 14-3-3-dependent activation and inhibition of arylalkylamine N-acetyltransferase mediated by phosphoserine-205
    Surajit Ganguly
    Section on Neuroendocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 4480, USA
    Proc Natl Acad Sci U S A 102:1222-7. 2005
    ..pS205 seems to be part of a previously unrecognized 14-3-3-binding motif-pS/pT (X1-2)-COOH, referred to here as mode III...
  56. ncbi Phospho-serine-118 estrogen receptor-alpha detection in human breast tumors in vivo
    Leigh Murphy
    Manitoba Institute of Cell Biology, Department of Biochemistry, University of Manitoba, Winnipeg, Manitoba, Canada
    Clin Cancer Res 10:1354-9. 2004
    ..IHC was used to determine the relationship of P-Ser(118)-ERalpha to known prognostic markers and active mitogen-activated protein kinase (MAPK; erk1/2) expression...
  57. ncbi Interaction of phosphorylated tyrosine hydroxylase with 14-3-3 proteins: evidence for a phosphoserine 40-dependent association
    R Kleppe
    Department of Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway
    J Neurochem 77:1097-107. 2001
    ..Both phosphoserine-specific antibodies directed against the 10 amino acids surrounding Ser19 or Ser40 of TH, and the ..
  58. ncbi Identification of a novel phosphorylation site in human androgen receptor by mass spectrometry
    Z Zhu
    Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, Ohio State University, Columbus, Ohio 43210, USA
    Biochem Biophys Res Commun 284:836-44. 2001
    ..These 21 peptides included 11 out of the 17 peptides detected by MALDI/TOF-MS. In addition, a novel serine phosphorylation site (Ser(308)) within the N-terminal transactivation domain of hAR was identified...
  59. pmc Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B
    Michael P Scheid
    Department of Experimental Therapeutics, University Health Network Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
    Mol Cell Biol 22:6247-60. 2002
    ....
  60. pmc Rsk2 allosterically activates estrogen receptor alpha by docking to the hormone-binding domain
    D E Clark
    Center for Cell Signaling and PharmaBiologicals, University of Virginia, Charlottesville, VA 22908 0577, USA
    EMBO J 20:3484-94. 2001
    ..The allosteric regulation of a target protein, independent of phosphorylation, may be paradigmatic of a general function for protein kinase docking sites...
  61. ncbi Regulation of the NMDA receptor complex and trafficking by activity-dependent phosphorylation of the NR2B subunit PDZ ligand
    Hee Jung Chung
    Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 24:10248-59. 2004
    ....
  62. ncbi Role of serine phosphorylation of Stat5a in prolactin-stimulated beta-casein gene expression
    H Yamashita
    United States Military Cancer Institute, Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Mol Cell Endocrinol 183:151-63. 2001
    ..We propose that serine phosphorylation within the transactivation domain may limit the activity of Stat5a in the absence of proper coactivation by glucocorticoid receptors...
  63. ncbi Phosphopeptide derivatization signatures to identify serine and threonine phosphorylated peptides by mass spectrometry
    M P Molloy
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109 0606, USA
    Anal Chem 73:5387-94. 2001
    ..The approach was further used to examine in vitro phosphorylation of recombinant human HSP22 by protein kinase C, revealing phosphorylation of Thr-63...
  64. pmc A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation
    Morten Frodin
    Department of Clinical Biochemistry, Glostrup Hospital, DK 2600 Glostrup, Denmark
    EMBO J 21:5396-407. 2002
    ..Here, we discover a pocket in the kinase domain of PDK1 that recognizes the phosphoserine/phosphothreonine in the hydrophobic motif by identifying two oppositely positioned arginine and lysine ..
  65. ncbi The use of in vitro peptide-library screens in the analysis of phosphoserine/threonine-binding domain structure and function
    Michael B Yaffe
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Biophys Biomol Struct 33:225-44. 2004
    b>Phosphoserine/threonine-binding domains integrate intracellular signal transduction events by forming multiprotein complexes with substrates of protein serine/threonine kinases...
  66. pmc Phosphorylation of androgen receptor isoforms
    Hao Yun Wong
    Department of Reproduction and Development, Erasmus MC, P O Box 1738, 3000 DR, Rotterdam, The Netherlands
    Biochem J 383:267-76. 2004
    ..After mutagenesis of these sites, no major change in functional activity of the AR was observed...
  67. ncbi Limited role of N-terminal phosphoserine residues in the activation of transcription by p53
    Mark W Jackson
    Department of Molecular Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Oncogene 23:4477-87. 2004
    ....
  68. ncbi Phosphoserine/threonine-binding domains
    M B Yaffe
    Center for Cancer Research E18 580, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Curr Opin Cell Biol 13:131-8. 2001
    ..directly result in the formation of multimolecular signaling complexes through specific interactions between phosphoserine/threonine (pSer/Thr)-binding modules and phosphorylated sequence motifs...
  69. ncbi Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line
    Hongjun Shu
    Protein Chemistry Laboratory, Alliance for Cellular Signaling, University of Texas Southwestern Medical Center, Dallas, TX 75390 9196, USA
    Mol Cell Proteomics 3:279-86. 2004
    ..The number of newly identified phosphoproteins indicates that B cell signaling pathways utilizing protein phosphorylation are likely to be more complex than previously appreciated...
  70. pmc Biosynthesis of phosphoserine in the Methanococcales
    Sunna Helgadóttir
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    J Bacteriol 189:575-82. 2007
    ..These methanogens charge tRNA(Cys) with l-phosphoserine, which is also an intermediate in the predicted pathways for serine and cystathionine biosynthesis...
  71. pmc RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea
    Jing Yuan
    Department of Molecular Biophysics and Biochemistry and Chemistry, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 103:18923-7. 2006
    ..for a different route present in these domains of life that requires the tRNA(Sec)-dependent conversion of O-phosphoserine (Sep) to Sec...
  72. pmc Biosynthesis of selenocysteine on its tRNA in eukaryotes
    Xue Ming Xu
    Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Biol 5:e4. 2007
    ..Conservation of the overall pathway of Sec biosynthesis suggests that this pathway is also active in other eukaryotes and archaea that synthesize selenoproteins...
  73. pmc Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells
    F Matsumura
    Department of Molecular Biology and Biochemistry, Rutgers University, Nelson Labs, Busch Campus, Piscataway, New Jersey 08855, USA
    J Cell Biol 140:119-29. 1998
    ....
  74. pmc Evidence for phosphorylation of serine 753 in CFTR using a novel metal-ion affinity resin and matrix-assisted laser desorption mass spectrometry
    D C Neville
    Department of Pharmaceutical Chemistry, University of California, San Francisco 94143 0446, USA
    Protein Sci 6:2436-45. 1997
    ..Peptide sequence and phosphorylation at residue 753Ser was confirmed using MALDI/CID analysis. This is the first report of phosphorylation of 753Ser in full-length CFTR...
  75. ncbi Serine phosphorylation-regulated ubiquitination and degradation of beta-catenin
    K Orford
    Lombardi Cancer Center, Georgetown University Medical Center, Washington, D C 20007, USA
    J Biol Chem 272:24735-8. 1997
    ..Mutation of a single serine within the ubiquitination targeting sequence prevents ubiquitination of beta-catenin. Mutations within the ubiquitination targeting sequence of beta-catenin may be oncogenic...
  76. ncbi Phosphorylation of the human estrogen receptor. Identification of hormone-regulated sites and examination of their influence on transcriptional activity
    P Le Goff
    Department of Physiology and Biophysics, University of Illinois, Urbana 61801
    J Biol Chem 269:4458-66. 1994
    ..Our studies identify several serines in the NH2-terminal portion of the human ER as being major hormone-regulated phosphorylation sites.(ABSTRACT TRUNCATED AT 250 WORDS)..
  77. pmc Protein phosphatase 2A dephosphorylation of phosphoserine 112 plays the gatekeeper role for BAD-mediated apoptosis
    Chi Wu Chiang
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Cell Biol 23:6350-62. 2003
    ..We report that phosphoserine 112 (pSer112) dephosphorylation functions as a gatekeeper for BAD-mediated apoptosis...
  78. ncbi Serine 167 is the major estradiol-induced phosphorylation site on the human estrogen receptor
    S F Arnold
    Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, New York 14642
    Mol Endocrinol 8:1208-14. 1994
    ..Western blot analysis and [32P]phosphate incorporation confirmed the presence of casein kinase II in Sf9 cells. These results demonstrate that the hER is phosphorylated on serine 167 by casein kinase II in a hormone-dependent manner...
  79. pmc 14-3-3:Shc scaffolds integrate phosphoserine and phosphotyrosine signaling to regulate phosphatidylinositol 3-kinase activation and cell survival
    Emma F Barry
    Cell Growth and Differentiation Laboratory, Division of Human Immunology, Hanson Institute, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia 5000, Australia
    J Biol Chem 284:12080-90. 2009
    ..How adaptor or scaffold proteins assemble signaling complexes through both phosphotyrosine and phosphoserine/threonine residues to regulate specific signaling pathways and biological responses is unclear...
  80. ncbi Chemical derivatization of phosphoserine and phosphothreonine containing peptides to increase sensitivity for MALDI-based analysis and for selectivity of MS/MS analysis
    Giorgio Arrigoni
    Department of Protein Technology, Lund University, Lund, Sweden
    Proteomics 6:757-66. 2006
    ..show an approach using a simple one-step beta-elimination/Michael addition reaction for the derivatization of phosphoserine and phosphothreonine...
  81. ncbi A new approach to phosphoserine and phosphothreonine analysis in peptides and proteins: chemical modification, enrichment via solid-phase reversible binding, and analysis by mass spectrometry
    Florian Thaler
    Biology Department, Discovery Research Oncology, Pharmacia Corporation, Viale Pasteur 10, 20014 Nerviano, Italy
    Anal Bioanal Chem 376:366-73. 2003
    beta-Elimination of the phosphate group on phosphoserine and phosphothreonine residues and addition of an alkyldithiol is a useful tool for analysis of the phosphorylation states of proteins and peptides...
  82. ncbi RNA-dependent cysteine biosynthesis in archaea
    Anselm Sauerwald
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Science 307:1969-72. 2005
    ..the corresponding activity from Methanocaldococcus jannaschii indicated that tRNA(Cys) becomes acylated with O-phosphoserine (Sep) but not with cysteine...
  83. pmc SPBP is a phosphoserine-specific repressor of estrogen receptor alpha
    Valentina Gburcik
    Department of Cell Biology, Sciences III, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland
    Mol Cell Biol 25:3421-30. 2005
    ..In a purified system, SPBP bound only the in vitro-phosphorylated form of the ERalpha AF1 or the phosphoserine mimic S118E, and the interaction domain could be mapped to a 42-amino-acid fragment of SPBP...
  84. ncbi A phosphoserine-lysine salt bridge within an alpha-helical peptide, the strongest alpha-helix side-chain interaction measured to date
    Neil Errington
    Faculty of Life Sciences, Jackson s Mill, The University of Manchester, Sackville Street, P O Box 88, Manchester M60 1QD, UK
    Biochemistry 44:7553-8. 2005
    ..The stabilization of the helix is considerable and can overcome the intrinsically low preference of phosphoserine for the interior of the helix...
  85. pmc The DNA invertase Gin of phage Mu: formation of a covalent complex with DNA via a phosphoserine at amino acid position 9
    A Klippel
    Institut für Genbiologische Forschung Berlin GmbH, FRG
    EMBO J 7:1229-37. 1988
    ..The covalent complex is formed through linkage of Gin to the 5' DNA phosphate at the site of the break via a phosphoserine. Extensive site-directed mutational analysis showed that all mutants altered at serine position 9 were ..
  86. ncbi Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates
    Andrew E H Elia
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 299:1228-31. 2003
    ..Cdks), we identified the polo-box domain (PBD) of the mitotic kinase polo-like kinase 1 (Plk1) as a specific phosphoserine (pSer) or phosphothreonine (pThr) binding domain and determined its optimal binding motif...
  87. ncbi Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody
    D L Clemm
    Endocrine Research, Ligand Pharmaceuticals, Inc, San Diego, California 92121, USA
    Mol Endocrinol 14:52-65. 2000
    ..That Ser 294 lies within an inhibitory domain that mediates the unique repressive functions of PR-A raises the possibility that differential phosphorylation of Ser 294 is involved in the distinct functional properties of PR-A and PR-B...
  88. ncbi Characterization of agonist stimulation of cAMP-dependent protein kinase and G protein-coupled receptor kinase phosphorylation of the beta2-adrenergic receptor using phosphoserine-specific antibodies
    Tuan M Tran
    The University of Texas, Houston, Medical School, Houston, TX 77225, USA
    Mol Pharmacol 65:196-206. 2004
    ..Ser356 of the human beta2AR overexpressed in human embryonic kidney (HEK) 293 cells were characterized using phosphoserine-specific antibodies...
  89. ncbi The BRCT domain is a phospho-protein binding domain
    Xiaochun Yu
    Department of Oncology, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Science 302:639-42. 2003
    ..Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control...
  90. ncbi Structural basis for phosphoserine-proline recognition by group IV WW domains
    M A Verdecia
    Structural Biology Laboratory, University of California, San Diego, La Jolla, California 92093, USA
    Nat Struct Biol 7:639-43. 2000
    ..To address the energetic and structural basis for WW domain recognition of phosphoserine (P.Ser)/phosphothreonine (P...
  91. ncbi Mutation of phosphoserine 389 affects p53 function in vivo
    M Hao
    Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, Houston, Texas 77030, USA
    J Biol Chem 271:29380-5. 1996
    ..Our data establish for the first time that phosphorylation of p53 at serine 389 is important in activating its function in vivo...
  92. pmc IkappaB kinase beta phosphorylates Dok1 serines in response to TNF, IL-1, or gamma radiation
    Sanghoon Lee
    International Agency for Research on Cancer, 150 cours Albert Thomas, 69008 Lyon, France
    Proc Natl Acad Sci U S A 101:17416-21. 2004
    ..These data indicate that IKKbeta phosphorylates Dok1 S(439)S(443) and S(446)S(450) after TNF-alpha, IL-1, or gamma-radiation and implicate the critical Dok1 serines in Dok1 effects after tyrosine kinase activation...
  93. pmc Stress- and mitogen-induced phosphorylation of the synapse-associated protein SAP90/PSD-95 by activation of SAPK3/p38gamma and ERK1/ERK2
    Guadalupe Sabio
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 380:19-30. 2004
    ....
  94. ncbi Identifying protein kinase substrates: hunting for the organ-grinder's monkeys
    Daniel C Berwick
    Department of Pharmacology, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Trends Biochem Sci 29:227-32. 2004
  95. pmc Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases
    Ivan Mijakovic
    Laboratoire de génétique des microorganismes, CNRS INRA INA PG UMR2585, 78850 Thiverval Grignon, France
    EMBO J 22:4709-18. 2003
    ..YwqE, which is encoded by the fourth gene of the B.subtilis ywqCDEF operon, also dephosphorylates P-Tyr-YwqD...
  96. ncbi Inhibition of insulin sensitivity by free fatty acids requires activation of multiple serine kinases in 3T3-L1 adipocytes
    Zhanguo Gao
    Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USA
    Mol Endocrinol 18:2024-34. 2004
    ....
  97. pmc Posttranslational modifications of bovine osteopontin: identification of twenty-eight phosphorylation and three O-glycosylation sites
    E S Sørensen
    Protein Chemistry Laboratory, University of Aarhus, Denmark
    Protein Sci 4:2040-9. 1995
    ..This conservation of serines, even in otherwise less well-conserved regions of the protein, indicates that the phosphorylation of osteopontin at specific sites is essential for the function of the protein...
  98. pmc GSK-3 beta targets Cdc25A for ubiquitin-mediated proteolysis, and GSK-3 beta inactivation correlates with Cdc25A overproduction in human cancers
    Tiebang Kang
    Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, MO 63110, USA
    Cancer Cell 13:36-47. 2008
    ..Importantly, a strong correlation between Cdc25A overproduction and GSK-3beta inactivation was observed in human tumor tissues, indicating that GSK-3beta inactivation may account for Cdc25A overproduction in a subset of human tumors...
  99. ncbi GSK-3-mediated phosphorylation enhances Maf-transforming activity
    Nathalie Rocques
    Institut Curie, Centre de Recherche, Orsay F 91405, France
    Mol Cell 28:584-97. 2007
    ..We further demonstrate that P/CAF protects MafA from ubiquitination and degradation, suggesting that, upon the release of the coactivator complex, MafA becomes polyubiquitinated and degraded to allow the response to terminate...
  100. ncbi Rho-kinase phosphorylates eNOS at threonine 495 in endothelial cells
    Masayuki Sugimoto
    Department of Vascular Surgery, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Showa Ku, Nagoya, Aichi 466 8550, Japan
    Biochem Biophys Res Commun 361:462-7. 2007
    ..Treatment with Y27632, a Rho-kinase inhibitor, suppressed thrombin-induced phosphorylation at Thr495. These results indicate that Rho-kinase can directly phosphorylate eNOS at Thr495 to suppress NO production in endothelium...
  101. pmc Aurora B phosphorylates multiple sites on mitotic centromere-associated kinesin to spatially and temporally regulate its function
    Xin Zhang
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Mol Biol Cell 18:3264-76. 2007
    ..Our study also shows that the interplay between multiple phosphorylation sites of MCAK may be critical to temporally and spatially control MCAK function...

Research Grants74

  1. METAL DEPENDENT COMPLEXES IN BLOOD COAGULATION
    Barbara Furie; Fiscal Year: 2002
    ..fragment 1 bound to calcium ions and glycerophosphoserine and/or a short chain phospholipid bearing the phosphoserine head group...
  2. THE ROLE OF PIN1 IN MITOSIS WITH DESIGNED INHIBITORS
    FELICIA ETZKORN; Fiscal Year: 2002
    DESCRIPTION: Pin 1, a newly discovered regulator of mitosis signal transduction, is an enzyme that acts on phosphoserine-proline amides...
  3. Platelet activation signaling via GPIb-IX and 14-3-3
    Xiaoping Du; Fiscal Year: 2007
    ..We have found that the cytoplasmic domain of GPIb-alpha binds to a phosphoserine-dependent signaling molecule, 14-3-3...
  4. Chemistry and biology of protein histidine phosphorylation
    Tom W Muir; Fiscal Year: 2011
    ..While protein phosphorylation is known to occur on several amino acids, it is fair to say that phosphoserine, phosphothreonine and phosphotyrosine capture most of the attention in the literature, at least as it pertains ..
  5. Role of L-3 phosphoserine phosphatase in epidermal apoptosis and carcinogenesis
    MICHAEL BACHELOR; Fiscal Year: 2007
    L-3-phosphoserine phosphatase (L3PSP) is a widely expressed member of the haloacid dehalogenase (HAD) superfamily and its enzyme activity is the irreversible and rate limiting step in L-serine biosynthesis...
  6. Role of L-3-phosphoserine phosphatase in epidermal growth and survival
    David Owens; Fiscal Year: 2007
    L-3-phosphoserine phosphatase (L3PSP) is a widely expressed member of the haloacid dehalogenase (HAD) superfamily and its enzyme activity is the irreversible and rate limiting step in L-serine biosynthesis...
  7. Serotonin N-acethyltransferase Regulation & Inhibition
    Philip Cole; Fiscal Year: 2007
    ..By incorporating non-hydrolyzable phosphono amino acid analogs in place of phosphothreonine and phosphoserine into serotonin N-acetyltransferase, the precise role of phosphorylation should be revealed...
  8. Pin1 regulation of prosurvival signalling in eosinophils
    James S Malter; Fiscal Year: 2010
    ..Pin1 binds to and catalyzes the cis-trans isomerization of phosphoserine-proline or phosphothreonine-proline (Ser/Thr-Pro) peptide bonds...
  9. Translating Mussel Adhesion
    JOHN HERBERT WAITE; Fiscal Year: 2010
    ..The specific aims here are to determine using mass spectrometry whether and to what extent phosphoserine and 4-hydroxyarginine are linked to mussel adhesion on different surfaces, characterize the specific protein-..
  10. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    Donald Defranco; Fiscal Year: 1993
    ..In this way dephosphorylation of specific phosphoserine residues within the GR that are associated with a recycling pathway may be identified...
  11. Mechanisms and regulation of serine resolvases
    Phoebe A Rice; Fiscal Year: 2010
    ..It is known that they form phosphoserine intermediates, but the structures available to date do not show a fully assembled active site, making it ..
  12. Designed Inhibitors of Pin1 in Mitosis
    FELICIA ETZKORN; Fiscal Year: 2009
    Pin1 is an enzyme that catalyzes isomerization of phosphoserine/threonine-proline (pSer/Thr-Pro) amides...
  13. Designed Inhibitors of Pin1 in Mitosis
    FELICIA ETZKORN; Fiscal Year: 2007
    Pin1 is an enzyme that catalyzes isomerization of phosphoserine/threonine-proline (pSer/Thr-Pro) amides...
  14. Structural Basis for Insulin Resistance Mediated by IRS-1 Serine Phosphorylation
    SANGYOUN PARK; Fiscal Year: 2007
    ..b>Phosphoserine residues have never been shown to bind PTB domains, but I hypothesize that this occurs because they are ..
  15. SPECIFICITY OF PROTEIN TYROSINE PHOSPHATASES
    Zhong Yin Zhang; Fiscal Year: 2001
    ..of the dual specificity phosphatases that can utilize substrates containing phosphotyrosine as well as phosphoserine/phosphothreonine...
  16. STUDIES OF PROTEINS INVOLVED IN DENTINOGENESIS
    William Butler; Fiscal Year: 2003
    ..Recently, we have identified DMP1 as a 150-200 kDa protein in dentin ECM. The large content of phosphoserine suggests that DMP1 may play an important role in mineral formation and growth and in bone resorption...
  17. Aptamers to distinguish functional modification of target proteins by affinity pr
    STEVEN SULJAK; Fiscal Year: 2007
    ..establishing reliable APCE methods for a selected peptide domain, specific modified peptides that incorporate phosphoserine and/or phosphothreonine will be investigated as models of typical post-translational modifications...
  18. Characterization of DMP1 A Dentin Phosphoprotein
    Anne George; Fiscal Year: 2006
    ..DMP1 is typically polyanionic having a high content of aspartic, glutamic and phosphoserine residues making it a protein highly suitable for calcium binding...
  19. PHOSPHOSERINE DEPENDENT ASSEMBLY OF SIGNALING COMPLEXES
    Michael Yaffe; Fiscal Year: 2003
    ..Yaffe and others have recently found that a variety of protein-protein complexes are formed through direct phosphoserine-mediated reactions, much like phosphotyrosine interactions seen in complexes involving SH2 and PTB domains...
  20. SMOOTH MUSCLE CELL GROWTH CONTROL BY HEPARAN SULFATES
    John Castellot; Fiscal Year: 2002
    ..signaling pathways by identifying and characterizing the function of heparin-regulated phosphotyrosine, phosphoserine, and phosphothreonine proteins, and by analyzing the effect of heparin cytosolic and nuclear calcium ..
  21. NEW INSIGHTS INTO ENZYME STRUCTURE/FUNCTION
    Evan Kantrowitz; Fiscal Year: 2001
    ..which the active site serine has been replaced in order to learn more about phosphoester hydrolysis without a phosphoserine intermediate, and (vi) continue to elucidate the contribution of individual amino acids and the metals towards ..