phosphoserine

Summary

Summary: The phosphoric acid ester of serine.

Top Publications

  1. ncbi Formation of D-serine from L-phosphoserine in brain synaptosomes
    P L Wood
    CoCensys, Inc, Irvine, California 92718, USA
    J Neurochem 67:1485-90. 1996
  2. ncbi Molecular plasticity in the rat auditory brainstem: modulation of expression and distribution of phosphoserine, phospho-CREB and TrkB after noise trauma
    Steffen A Michler
    Neurobiological Research Laboratory, Department of Otorhinolaryngology, University of Freiburg, Freiburg, Germany
    Audiol Neurootol 8:190-206. 2003
  3. ncbi The BRCT domain is a phospho-protein binding domain
    Xiaochun Yu
    Department of Oncology, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Science 302:639-42. 2003
  4. ncbi Function of WW domains as phosphoserine- or phosphothreonine-binding modules
    P J Lu
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Science 283:1325-8. 1999
  5. ncbi Phosphoserine/threonine-binding domains
    M B Yaffe
    Center for Cancer Research E18 580, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Curr Opin Cell Biol 13:131-8. 2001
  6. ncbi The structural basis for 14-3-3:phosphopeptide binding specificity
    M B Yaffe
    Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Cell 91:961-71. 1997
  7. pmc Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine
    Steven C Bremmer
    Department of Biochemistry, Purdue Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 287:1662-9. 2012
  8. pmc A Smad action turnover switch operated by WW domain readers of a phosphoserine code
    Eric Aragon
    Structural and Computational Biology Programme, Institute for Research in Biomedicine, Barcelona, Spain
    Genes Dev 25:1275-88. 2011
  9. pmc CDK targets Sae2 to control DNA-end resection and homologous recombination
    Pablo Huertas
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 455:689-92. 2008
  10. ncbi Regulation of plant symbiosis receptor kinase through serine and threonine phosphorylation
    Satoko Yoshida
    Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH, United Kingdom
    J Biol Chem 280:9203-9. 2005

Detail Information

Publications229 found, 100 shown here

  1. ncbi Formation of D-serine from L-phosphoserine in brain synaptosomes
    P L Wood
    CoCensys, Inc, Irvine, California 92718, USA
    J Neurochem 67:1485-90. 1996
    ..The rate-limiting step in the synthesis of serine is the hydrolysis of phosphoserine by phosphoserine phosphatase (EC 3.1.3.3)...
  2. ncbi Molecular plasticity in the rat auditory brainstem: modulation of expression and distribution of phosphoserine, phospho-CREB and TrkB after noise trauma
    Steffen A Michler
    Neurobiological Research Laboratory, Department of Otorhinolaryngology, University of Freiburg, Freiburg, Germany
    Audiol Neurootol 8:190-206. 2003
    ..for tyrosine receptor kinase B in the ipsilateral VCN between day 3 and day 21 after trauma and an increase in phosphoserine immunostaining in the neuropil of the ipsilateral VCN and in neurons of the contralateral lateral superior ..
  3. ncbi The BRCT domain is a phospho-protein binding domain
    Xiaochun Yu
    Department of Oncology, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Science 302:639-42. 2003
    ..Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control...
  4. ncbi Function of WW domains as phosphoserine- or phosphothreonine-binding modules
    P J Lu
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Science 283:1325-8. 1999
    ..Although phosphotyrosine-binding modules have been well-characterized, phosphoserine- or phosphothreonine-binding modules have not been described...
  5. ncbi Phosphoserine/threonine-binding domains
    M B Yaffe
    Center for Cancer Research E18 580, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Curr Opin Cell Biol 13:131-8. 2001
    ..directly result in the formation of multimolecular signaling complexes through specific interactions between phosphoserine/threonine (pSer/Thr)-binding modules and phosphorylated sequence motifs...
  6. ncbi The structural basis for 14-3-3:phosphopeptide binding specificity
    M B Yaffe
    Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Cell 91:961-71. 1997
    The 14-3-3 family of proteins mediates signal transduction by binding to phosphoserine-containing proteins...
  7. pmc Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine
    Steven C Bremmer
    Department of Biochemistry, Purdue Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 287:1662-9. 2012
    ..We show that budding yeast Cdc14 possesses a strong and unusual preference for phosphoserine over phosphothreonine at Pro-directed sites in vitro...
  8. pmc A Smad action turnover switch operated by WW domain readers of a phosphoserine code
    Eric Aragon
    Structural and Computational Biology Programme, Institute for Research in Biomedicine, Barcelona, Spain
    Genes Dev 25:1275-88. 2011
    ..Thus, a Smad phosphoserine code and a set of WW domain code readers provide an efficient solution to the problem of coupling TGF-β ..
  9. pmc CDK targets Sae2 to control DNA-end resection and homologous recombination
    Pablo Huertas
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 455:689-92. 2008
    ..These findings therefore provide a mechanistic basis for cell-cycle control of DSB repair and highlight the importance of regulating DSB resection...
  10. ncbi Regulation of plant symbiosis receptor kinase through serine and threonine phosphorylation
    Satoko Yoshida
    Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH, United Kingdom
    J Biol Chem 280:9203-9. 2005
    ..Despite the distinct domain architecture of receptor-like kinases versus Pelle/interleukin-1 receptor associated kinase, our results suggest the existence of conserved activation mechanisms...
  11. ncbi Structural basis for phosphoserine-proline recognition by group IV WW domains
    M A Verdecia
    Structural Biology Laboratory, University of California, San Diego, La Jolla, California 92093, USA
    Nat Struct Biol 7:639-43. 2000
    ..To address the energetic and structural basis for WW domain recognition of phosphoserine (P.Ser)/phosphothreonine (P...
  12. pmc Aging reveals a role for nigral tyrosine hydroxylase ser31 phosphorylation in locomotor activity generation
    Michael F Salvatore
    Department of Pharmacology, Toxicology, and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA
    PLoS ONE 4:e8466. 2009
    ....
  13. pmc Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells
    Tae Hong Kang
    Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang 790 784, Republic of Korea
    Mol Cell Biol 27:8533-46. 2007
    ..Overexpression of VRK1 resulted in a dramatic condensation of nuclei. Our findings collectively support a role of VRK1 as a novel mitotic histone H3 kinase in mammals...
  14. ncbi Expanding the genetic code of Escherichia coli with phosphoserine
    Hee Sung Park
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Science 333:1151-4. 2011
    O-Phosphoserine (Sep), the most abundant phosphoamino acid in the eukaryotic phosphoproteome, is not encoded in the genetic code, but synthesized posttranslationally...
  15. ncbi A p53 amino-terminal nuclear export signal inhibited by DNA damage-induced phosphorylation
    Y Zhang
    Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, and Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, NC 27599 7295, USA
    Science 292:1910-5. 2001
    ..Serine-15-phosphorylated p53 induced by ultraviolet irradiation was not exported. Thus, DNA damage-induced phosphorylation may achieve optimal p53 activation by inhibiting both MDM2 binding to, and the nuclear export of, p53...
  16. ncbi Identification of Tim4 as a phosphatidylserine receptor
    Masanori Miyanishi
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida, Sakyo ku, Kyoto 606 8501, Japan
    Nature 450:435-9. 2007
    ..These results indicate that Tim4 and Tim1 are phosphatidylserine receptors for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved...
  17. ncbi KAP1, a novel substrate for PIKK family members, colocalizes with numerous damage response factors at DNA lesions
    David E White
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 66:11594-9. 2006
    ..This discovery provides a novel link between chromatin-mediated transcriptional repression and the recognition/repair of DNA, which must be accomplished by the cellular DNA damage response...
  18. pmc Role of focal adhesion kinase Ser-732 phosphorylation in centrosome function during mitosis
    Ann Y J Park
    Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 284:9418-25. 2009
    ..Together, these studies demonstrated a novel role for Ser-732 phosphorylation of FAK in the regulation of centrosome function during mitosis, which may contribute to EC proliferation and angiogenesis...
  19. ncbi ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity
    Elisa Oricchio
    Institute of Biology and Molecular Pathology, National Research Council, Rome, Italy
    Cell Cycle 5:88-92. 2006
    ....
  20. pmc AMPK directly inhibits NDPK through a phosphoserine switch to maintain cellular homeostasis
    Rob U Onyenwoke
    Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Mol Biol Cell 23:381-9. 2012
    ....
  21. pmc The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity
    David Shahbazian
    Department of Biochemistry, McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    EMBO J 25:2781-91. 2006
    ..Phosphorylation of eIF4B on Ser422 by RSK and S6K is physiologically significant, as it increases the interaction of eIF4B with the eukaryotic translation initiation factor 3...
  22. pmc Dual recognition of phosphoserine and phosphotyrosine in histone variant H2A.X by DNA damage response protein MCPH1
    Namit Singh
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
    Proc Natl Acad Sci U S A 109:14381-6. 2012
    ..X phosphorylation marks. We show that MCPH1 recruitment to sites of DNA damage is linked to both states of H2A.X...
  23. pmc HECT domain-containing E3 ubiquitin ligase Nedd4 interacts with and ubiquitinates Sprouty2
    Francis Edwin
    Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois 60153, USA
    J Biol Chem 285:255-64. 2010
    ..This is the first report demonstrating the regulation of cellular Spry content and its ability to modulate receptor tyrosine kinase signaling by a HECT domain-containing E3 ubiquitin ligase...
  24. pmc Androgen receptor phosphorylation and stabilization in prostate cancer by cyclin-dependent kinase 1
    Shaoyong Chen
    Hematology Oncology Division, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 103:15969-74. 2006
    ....
  25. pmc Cysteine biosynthesis in Trichomonas vaginalis involves cysteine synthase utilizing O-phosphoserine
    Gareth D Westrop
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, Scotland, United Kingdom
    J Biol Chem 281:25062-75. 2006
    ..vaginalis relies upon cysteine synthase. The enzyme (TvCS1) can use either O-acetylserine or O-phosphoserine as substrates. The K(m) values of the enzyme for sulfide are very low (0...
  26. pmc A myristoyl/phosphoserine switch controls cAMP-dependent protein kinase association to membranes
    Ece C Gaffarogullari
    Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 411:823-36. 2011
    ....
  27. ncbi Cyclic AMP-dependent protein kinase phosphorylation of Drp1 regulates its GTPase activity and mitochondrial morphology
    Chuang Rung Chang
    Cellular Neurology Unit, NINDS, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 282:21583-7. 2007
    ..Thus, protein phosphorylation at Ser(637) results in clear alterations in Drp1 function and mitochondrial morphology that are likely involved in dynamic regulation of mitochondrial division in cells...
  28. pmc Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor
    Fabrice Le Boeuf
    Le Centre de Recherche en Cancérologie de l Université Laval, Quebec, Quebec G1R 2J6, Canada
    Mol Biol Cell 17:3508-20. 2006
    ..Then, activated Pyk2 triggers the phosphorylation of FAK on Tyr407, promoting cell migration...
  29. pmc Ser364 of connexin43 and the upregulation of gap junction assembly by cAMP
    E M TenBroek
    Department of Genetics, Cell Biology, and Development, University of Minnesota, St Paul, MN 55108, USA
    J Cell Biol 155:1307-18. 2001
    ..Furthermore, GJ assembly between S364ACx43/WT fibroblasts could be stimulated by 8Br-cAMP, but could not be between S364PCx43/WT fibroblasts. Thus, S364PCx43 interferes with enhanced GJ assembly when coexpressed with WTCx43...
  30. pmc Melatonin synthesis: 14-3-3-dependent activation and inhibition of arylalkylamine N-acetyltransferase mediated by phosphoserine-205
    Surajit Ganguly
    Section on Neuroendocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 4480, USA
    Proc Natl Acad Sci U S A 102:1222-7. 2005
    ..pS205 seems to be part of a previously unrecognized 14-3-3-binding motif-pS/pT (X1-2)-COOH, referred to here as mode III...
  31. ncbi The use of in vitro peptide-library screens in the analysis of phosphoserine/threonine-binding domain structure and function
    Michael B Yaffe
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Biophys Biomol Struct 33:225-44. 2004
    b>Phosphoserine/threonine-binding domains integrate intracellular signal transduction events by forming multiprotein complexes with substrates of protein serine/threonine kinases...
  32. ncbi Limited role of N-terminal phosphoserine residues in the activation of transcription by p53
    Mark W Jackson
    Department of Molecular Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Oncogene 23:4477-87. 2004
    ....
  33. ncbi MDM2-regulated degradation of HIPK2 prevents p53Ser46 phosphorylation and DNA damage-induced apoptosis
    Cinzia Rinaldo
    Department of Experimental Oncology, Regina Elena Cancer Institute, Via delle Messi d Oro 156, 00158 Rome, Italy
    Mol Cell 25:739-50. 2007
    ....
  34. ncbi Crystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition
    R Dajani
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, United Kingdom
    Cell 105:721-32. 2001
    ....
  35. ncbi Reduced activation of phosphatidylinositol-3 kinase and increased serine 636 phosphorylation of insulin receptor substrate-1 in primary culture of skeletal muscle cells from patients with type 2 diabetes
    Karim Bouzakri
    INSERM U449 and CRNHL, IFR 62, R Laennec Medical Faculty, F 69370 Lyon Cedex 08, France
    Diabetes 52:1319-25. 2003
    ..Moreover, increased MAPK activity seems to play a role in the phosphorylation of IRS-1 on serine residue in human muscle cells...
  36. pmc Inhibition of PI3K binding to activators by serine phosphorylation of PI3K regulatory subunit p85alpha Src homology-2 domains
    Jennifer Y Lee
    Division of Signal Transduction, Cancer Center, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 108:14157-62. 2011
    ..These results reveal cross talk between PKC signaling and PI3K signaling that impairs PI3K pathway activation under conditions of persistent PKC (and protein kinase D) activity...
  37. ncbi Serine phosphorylation-regulated ubiquitination and degradation of beta-catenin
    K Orford
    Lombardi Cancer Center, Georgetown University Medical Center, Washington, D C 20007, USA
    J Biol Chem 272:24735-8. 1997
    ..Mutation of a single serine within the ubiquitination targeting sequence prevents ubiquitination of beta-catenin. Mutations within the ubiquitination targeting sequence of beta-catenin may be oncogenic...
  38. pmc SPBP is a phosphoserine-specific repressor of estrogen receptor alpha
    Valentina Gburcik
    Department of Cell Biology, Sciences III, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland
    Mol Cell Biol 25:3421-30. 2005
    ..In a purified system, SPBP bound only the in vitro-phosphorylated form of the ERalpha AF1 or the phosphoserine mimic S118E, and the interaction domain could be mapped to a 42-amino-acid fragment of SPBP...
  39. ncbi PhosphoSerine/threonine binding domains: you can't pSERious?
    M B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Structure 9:R33-8. 2001
    ..Here, we review the insights into serine/threonine phosphorylation-dependent signal transduction processes provided by structures of several of these proteins and their complexes...
  40. pmc Phosphorylation of Plk1 at Ser326 regulates its functions during mitotic progression
    J Tang
    Department of Biochemistry, Cancer Center, Purdue University, West Lafayette, IN 47907, USA
    Oncogene 27:6635-45. 2008
    ..Only Plk1-S326E, but not the Plk1-S326A, efficiently rescued the p38 or MK2-depletion-induced mitotic defects, further solidifying the requirement of S326 phosphorylation during mitotic progression...
  41. pmc ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling
    Thomas Stiff
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, East Sussex, UK
    EMBO J 25:5775-82. 2006
    ..Our findings provide insight into the interplay between the PIKK damage response pathways...
  42. ncbi A phosphoserine-lysine salt bridge within an alpha-helical peptide, the strongest alpha-helix side-chain interaction measured to date
    Neil Errington
    Faculty of Life Sciences, Jackson s Mill, The University of Manchester, Sackville Street, P O Box 88, Manchester M60 1QD, UK
    Biochemistry 44:7553-8. 2005
    ..The stabilization of the helix is considerable and can overcome the intrinsically low preference of phosphoserine for the interior of the helix...
  43. ncbi Positional effects of phosphoserine on β-hairpin stability
    Alexander J Riemen
    Department of Chemistry, CB 3290, University of North Carolina, Chapel Hill, NC 27599, USA
    Org Biomol Chem 8:5411-7. 2010
    A disruptive interaction of phosphoserine with tryptophan in peptides that autonomously fold into a β-hairpin structure in aqueous solution was explored in a positional context within the hairpin structure...
  44. ncbi Cutting Edge: Internalization of transduced E-selectin by cultured human endothelial cells: comparison of dermal microvascular and umbilical vein cells and identification of a phosphoserine-type di-leucine motif
    Martin S Kluger
    Interdepartmental Program in Vascular Biology and Transplantation, Department of Dermatology, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    J Immunol 168:2091-5. 2002
    ..Control of E-selectin surface expression appears to be phosphoserine dependent, since alanine but not aspartic acid substitution for S581 slows E-selectin internalization.
  45. ncbi Spindle checkpoint protein hMad2 and histone H3 phosphoserine 10 mitosis marker in pediatric solid tumors
    Halliday A Idikio
    Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada
    Anticancer Res 26:4687-94. 2006
    ..hMad2 expression paralleled overall histone H3 phosphoserine 10 expression in most tumor types. Low hMad2 score tumors (0-3.5) exhibited lower complete response (66...
  46. ncbi A Fourier-transform infrared spectroscopic study of the phosphoserine residues in hen egg phosvitin and ovalbumin
    J M Sanchez-Ruiz
    School of Basic Life Sciences, Division of Molecular Biology and Biochemistry, University of Missouri, Kansas City 64110
    Biochemistry 27:3338-42. 1988
    ..Chem. Phys. 8, 435-498] can be identified easily in the 1300-930 cm-1 region in spectra of solutions of O-phosphoserine and phosvitin, a highly phosphorylated protein...
  47. pmc The 14-3-3tau phosphoserine-binding protein is required for cardiomyocyte survival
    Jeffrey M C Lau
    Center for Cardiovascular Research, Washington University School of Medicine, 660 South Euclid Ave, St Louis, MO 63110, USA
    Mol Cell Biol 27:1455-66. 2007
    14-3-3 family members are intracellular dimeric phosphoserine-binding proteins that regulate signal transduction, cell cycle, apoptotic, and metabolic cascades...
  48. ncbi Phosphorylation of smooth myosin light chain kinase by smooth muscle Ca2+/calmodulin-dependent multifunctional protein kinase
    M Ikebe
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106
    J Biol Chem 265:8975-8. 1990
    ..The preferred site was identified as serine 512 and the second site as serine 525. These sites are the same as the sites phosphorylated by cAMP-dependent protein kinase...
  49. ncbi Genetic and post-mortem mRNA analysis of the 14-3-3 genes that encode phosphoserine/threonine-binding regulatory proteins in schizophrenia and bipolar disorder
    Albert H C Wong
    Centre for Addiction and Mental Health, Faculty of Medicine, University of Toronto, Canada
    Schizophr Res 78:137-46. 2005
    ..The 14-3-3 genes code for a family of proteins that bind to and regulate other proteins, and they modulate neurodevelopment, cell-division, signal transduction and gene transcription...
  50. pmc Cyclin-dependent kinase (CDK) phosphorylation destabilizes somatic Wee1 via multiple pathways
    Nobumoto Watanabe
    Antibiotics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 102:11663-8. 2005
    ..Using a specific inhibitor of CK2, we showed that the phosphorylation-dependent degradation of Wee1A is important for the proper onset of mitosis...
  51. pmc Phosphorylation of androgen receptor isoforms
    Hao Yun Wong
    Department of Reproduction and Development, Erasmus MC, P O Box 1738, 3000 DR, Rotterdam, The Netherlands
    Biochem J 383:267-76. 2004
    ..After mutagenesis of these sites, no major change in functional activity of the AR was observed...
  52. ncbi Phosphopeptide derivatization signatures to identify serine and threonine phosphorylated peptides by mass spectrometry
    M P Molloy
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109 0606, USA
    Anal Chem 73:5387-94. 2001
    ..The approach was further used to examine in vitro phosphorylation of recombinant human HSP22 by protein kinase C, revealing phosphorylation of Thr-63...
  53. pmc Structural units important for activity of a novel-type phosphoserine phosphatase from Hydrogenobacter thermophilus TK-6 revealed by crystal structure analysis
    Yoko Chiba
    Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 288:11448-58. 2013
    Novel-type serine-synthesizing enzymes, termed metal-independent phosphoserine phosphatases (iPSPs), were recently identified and characterized from Hydrogenobacter thermophilus, a chemolithoautotrophic bacterium belonging to the order ..
  54. ncbi Effect of modification of hydroxyapatite/collagen composites with sodium citrate, phosphoserine, phosphoserine/RGD-peptide and calcium carbonate on bone remodelling
    Wolfgang Schneiders
    Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus, Fetscherstrasse 74, 01307 Dresden, Germany
    Bone 40:1048-59. 2007
    ..containing type I collagen (HA/Coll) and its modifications with sodium citrate (CI), calcium carbonate (CA), phosphoserine (P) and phosphoserine plus RGD-peptide (RGD)...
  55. ncbi Modelling of the gas-phase phosphate group loss and rearrangement in phosphorylated peptides
    Marko Rozman
    Laboratory for Chemical Kinetics and Atmospheric Chemistry, Ruđer Bošković Institute, Bijenicka 54, HR 10002, Zagreb, Croatia
    J Mass Spectrom 46:949-55. 2011
    ..The fragmentation dynamics of phosphoserine versus phosphothreonine containing peptides suggests that H(3)PO(4) neutral loss from phosphothreonine ..
  56. ncbi Inhibition of the Bcr-Abl oncoprotein by Bcr requires phosphoserine 354
    Natalyn Hawk
    Department of Molecular Pathology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer Res 62:386-90. 2002
    ..The autokinase product contained a M(r) 45,000-47,000 and 55,000 protein. Both species were detected by a Bcr phosphoserine 354 sequence-specific antibody...
  57. ncbi Phosphoserine-dependent regulation of protein-protein interactions in the Smad pathway
    Jeffrey L Wrana
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Structure 10:5-7. 2002
    ..The activity of this pathway is tightly controlled by serine phosphorylation, which plays a key role in regulating protein-protein interactions that are critical in the elaboration of transcriptional responses...
  58. ncbi Sequences of sixteen phosphoserine peptides from ovalbumins of eight species
    J Y Henderson
    Eur J Biochem 114:439-50. 1981
    b>Phosphoserine peptides have been isolated by a diagonal electrophoresis method from proteolytic digests of ovalbumins from hen, grouse, turkey, golden pheasant, magpie goose, chinese goose, Aylesbury duck and fulvous whistling duck...
  59. pmc Interrogation of MDM2 phosphorylation in p53 activation using native chemical ligation: the functional role of Ser17 phosphorylation in MDM2 reexamined
    Changyou Zhan
    Institute of Human Virology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    J Am Chem Soc 134:6855-64. 2012
    ..Our findings demonstrate that Ser17 phosphorylation is functionally neutral with respect to p53 binding, suggesting that MDM2 phosphorylation at a single site is unlikely to play a dominant role in stress-induced p53 activation...
  60. ncbi NMR spectroscopy of 14-3-3ζ reveals a flexible C-terminal extension: differentiation of the chaperone and phosphoserine-binding activities of 14-3-3ζ
    Danielle M Williams
    School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia
    Biochem J 437:493-503. 2011
    Intracellular 14-3-3 proteins bind to many proteins, via a specific phosphoserine motif, regulating diverse cellular tasks including cell signalling and disease progression...
  61. ncbi Hyaluronan-dependent cell migration can be blocked by a CD44 cytoplasmic domain peptide containing a phosphoserine at position 325
    D Peck
    Department of Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, UK
    J Cell Sci 111:1595-601. 1998
    ..Peptides containing a phosphoserine at residue 325 are efficient blockers of CD44-mediated cell migration but do not reduce CD44 expression or its ..
  62. ncbi Mechanistic insights into the multistage gas-phase fragmentation behavior of phosphoserine- and phosphothreonine-containing peptides
    Amanda M Palumbo
    Department of Chemistry Michigan State University, East Lansing, Michigan 48824, USA
    J Proteome Res 7:771-9. 2008
    ..examined the multistage collision-induced dissociation (CID) behavior of a set of singly and doubly charged phosphoserine- and phosphothreonine-containing peptide ions, as well as their regioselectively or uniformly deuterated ..
  63. ncbi Phosphorylation of bovine adrenal chromaffin cell tyrosine hydroxylase. Temporal correlation of acetylcholine's effect on site phosphorylation, enzyme activation, and catecholamine synthesis
    J C Waymire
    Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225
    J Biol Chem 263:12439-47. 1988
    ....
  64. ncbi Quantitative and selective fluorophore labeling of phosphoserine on peptides and proteins: characterization at the attomole level by capillary electrophoresis and laser-induced fluorescence
    P Fadden
    Department of Pharmacology, University of Virginia, Charlottesville 22908, USA
    Anal Biochem 225:81-8. 1995
    Reaction conditions were defined for the selective quantitative derivatization and fluorophore labeling of phosphoserine residues on peptides and proteins...
  65. pmc Involvement of protein kinase C and Src tyrosine kinase in acute tolerance to ethanol inhibition of spinal NMDA-induced pressor responses in rats
    W K Hsieh
    Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan
    Br J Pharmacol 158:806-18. 2009
    ....
  66. ncbi Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates
    Andrew E H Elia
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 299:1228-31. 2003
    ..Cdks), we identified the polo-box domain (PBD) of the mitotic kinase polo-like kinase 1 (Plk1) as a specific phosphoserine (pSer) or phosphothreonine (pThr) binding domain and determined its optimal binding motif...
  67. ncbi Critical role of WW domain phosphorylation in regulating phosphoserine binding activity and Pin1 function
    Pei Jung Lu
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 277:2381-4. 2002
    b>Phosphoserine-binding modules help determine the specificity of signal transduction events...
  68. ncbi Regulation of the NMDA receptor complex and trafficking by activity-dependent phosphorylation of the NR2B subunit PDZ ligand
    Hee Jung Chung
    Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 24:10248-59. 2004
    ....
  69. ncbi p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment
    Simona Saccani
    Institute for Research in Biomedicine, Via Vela 6, CH 6501, Bellinzona, Switzerland
    Nat Immunol 3:69-75. 2002
    ..These results show that p38 plays an additional role in the induction of the inflammatory and immune response: the regulation of NF-kappa B recruitment to selected chromatin targets...
  70. ncbi DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage
    Naoe Taira
    Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, Yushima 1 5 45, Bunkyo ku, Tokyo 113 8510, Japan
    Mol Cell 25:725-38. 2007
    ..Consistent with these results, DYRK2 induces p53AIP1 expression and apoptosis in a Ser46 phosphorylation-dependent manner. These findings indicate that DYRK2 regulates p53 to induce apoptosis in response to DNA damage...
  71. pmc A growth factor-dependent nuclear kinase phosphorylates p27(Kip1) and regulates cell cycle progression
    Manfred Boehm
    Cardiovascular Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 21:3390-401. 2002
    ..Through phosphorylation of p27(Kip1) on S10, hKIS regulates cell cycle progression in response to mitogens...
  72. ncbi Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling
    J W Wu
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USA
    Mol Cell 8:1277-89. 2001
    ..8 A resolution, reveals the formation of a homotrimer mediated by the C-terminal phosphoserine (pSer) residues...
  73. pmc RIM1alpha phosphorylation at serine-413 by protein kinase A is not required for presynaptic long-term plasticity or learning
    Pascal S Kaeser
    Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 105:14680-5. 2008
    ....
  74. pmc Suppression of RhoG activity is mediated by a syndecan 4-synectin-RhoGDI1 complex and is reversed by PKCalpha in a Rac1 activation pathway
    Arye Elfenbein
    Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
    J Cell Biol 186:75-83. 2009
    ..Thus, FGF2-induced Rac1 activation depends on the suppression of RhoG by a previously uncharacterized ternary S4-synectin-RhoGDI1 protein complex and activation via PKCalpha...
  75. pmc Nucleoside diphosphate kinase from Escherichia coli
    N Almaula
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Bacteriol 177:2524-9. 1995
    ..These results are discussed in light of the recent report of N. J. MacDonald et al. on the autophosphorylation of human NDP kinase (J. Biol. Chem. 268:25780-25789, 1993)...
  76. ncbi Inhibition of myosin light chain kinase by p21-activated kinase
    L C Sanders
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 283:2083-5. 1999
    ..These data indicate that MLCK is a target for PAKs and that PAKs may regulate cytoskeletal dynamics by decreasing MLCK activity and MLC phosphorylation...
  77. ncbi Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys)
    Chun Mei Zhang
    Department of Biochemistry and Molecular Biology, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
    Nat Struct Mol Biol 15:507-14. 2008
    ..Cys-tRNA(Cys) by an indirect pathway, whereby O-phosphoseryl-tRNA synthetase (SepRS) acylates tRNA(Cys) with phosphoserine (Sep), and Sep-tRNA-Cys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine...
  78. ncbi Glycogen synthase kinase-3 beta is a dual specificity kinase differentially regulated by tyrosine and serine/threonine phosphorylation
    Q M Wang
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202 5122
    J Biol Chem 269:14566-74. 1994
    ..Phosphorylation at different residues differentially controls enzyme activity, Ser/Thr phosphorylation causing inactivation and Tyr phosphorylation resulting in increased activity...
  79. ncbi The phosphoserine-585-dependent pathway of the GM-CSF/IL-3/IL-5 receptors mediates hematopoietic cell survival through activation of NF-kappaB and induction of bcl-2
    Mark A Guthridge
    Cytokine Receptor Laboratory, Department of Human Immunology, Institute of Medical and Veterinary Science, Frome Rd, Adelaide, South Australia, Australia 5000
    Blood 103:820-7. 2004
    ..These results demonstrate that both serine and tyrosine phosphorylation pathways play a role in hematopoietic cell survival, are initially independent of each other, and converge on NF-kappaB to promote bcl-2 expression...
  80. ncbi BRCT repeats as phosphopeptide-binding modules involved in protein targeting
    Isaac A Manke
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 302:636-9. 2003
    ..BRCA1 carboxyl-terminal) domains in PTIP (Pax transactivation domain-interacting protein) and in BRCA1 as phosphoserine- or phosphothreonine-specific binding modules that recognize substrates phosphorylated by the kinases ATM (..
  81. pmc Stt7-dependent phosphorylation during state transitions in the green alga Chlamydomonas reinhardtii
    Sylvain Lemeille
    Department of Molecular Biology and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
    Mol Cell Proteomics 9:1281-95. 2010
    ..Moreover, we also identified phosphorylation sites that are redox (state 2)-dependent but independent of Stt7 and additional phosphorylation sites that are redox-independent...
  82. ncbi Isoform-specific defects of insulin stimulation of Akt/protein kinase B (PKB) in skeletal muscle cells from type 2 diabetic patients
    D Cozzone
    INSERM, U 870, IFR62, Faculté de médecine Lyon Sud, Chemin du Grand Revoyet, F 69600 Oullins, France
    Diabetologia 51:512-21. 2008
    ..Because three Akt isoforms exist, each having a distinct physiological role, we investigated the contribution of isoform-specific defects to insulin signalling in human muscle...
  83. ncbi Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1
    B Chu
    Dana Farber Cancer Institute and Joint Center for Radiation Therapy, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 271:30847-57. 1996
    ....
  84. ncbi Phosphoserine--a convenient compound for modification of calcium phosphate bone cement collagen composites
    A Reinstorf
    Dresden University of Technology, Department of Materials Science, Max Bergmann Center of Biomaterials, Budapester Str 27, 01062 Dresden, Germany
    J Mater Sci Mater Med 15:451-5. 2004
    ..Furthermore, the proliferation and differentiation were found to be enhanced on the PS-modified material...
  85. ncbi 14-3-3 (epsilon) interacts with the insulin-like growth factor I receptor and insulin receptor substrate I in a phosphoserine-dependent manner
    A Craparo
    Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 272:11663-9. 1997
    ..We present evidence to suggest that 14-3-3 interacts with phosphoserine residues within the COOH terminus of the IGFIR...
  86. ncbi Fluorescent caged phosphoserine peptides as probes to investigate phosphorylation-dependent protein associations
    M Eugenio Vazquez
    Department of Chemistry and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 125:10150-1. 2003
    ..At the same time, the newly developed photolabile 1-(2-nitrophenyl)ethyl-caged phosphoserine allows control of the release of the biologically active ligand through unmasking of the key phosphoserine ..
  87. ncbi Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding
    XiaoZhe Wang
    Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    J Biol Chem 281:782-91. 2006
    ..These experiments indicate a novel mechanism for the regulation of HSF1 by proinflammatory signaling and may permit HSF1 to respond rapidly to extracellular events, permitting optimal physiological regulation...
  88. pmc Protein phosphatase 2A dephosphorylation of phosphoserine 112 plays the gatekeeper role for BAD-mediated apoptosis
    Chi Wu Chiang
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Cell Biol 23:6350-62. 2003
    ..We report that phosphoserine 112 (pSer112) dephosphorylation functions as a gatekeeper for BAD-mediated apoptosis...
  89. ncbi Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line
    Hongjun Shu
    Protein Chemistry Laboratory, Alliance for Cellular Signaling, University of Texas Southwestern Medical Center, Dallas, TX 75390 9196, USA
    Mol Cell Proteomics 3:279-86. 2004
    ..The number of newly identified phosphoproteins indicates that B cell signaling pathways utilizing protein phosphorylation are likely to be more complex than previously appreciated...
  90. ncbi RNA-dependent cysteine biosynthesis in archaea
    Anselm Sauerwald
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Science 307:1969-72. 2005
    ..the corresponding activity from Methanocaldococcus jannaschii indicated that tRNA(Cys) becomes acylated with O-phosphoserine (Sep) but not with cysteine...
  91. ncbi Phospho-serine-118 estrogen receptor-alpha detection in human breast tumors in vivo
    Leigh Murphy
    Manitoba Institute of Cell Biology, Department of Biochemistry, University of Manitoba, Winnipeg, Manitoba, Canada
    Clin Cancer Res 10:1354-9. 2004
    ..To gain insight into possible roles for P-Ser(118)-ERalpha in human breast cancer in vivo...
  92. ncbi A new approach to phosphoserine and phosphothreonine analysis in peptides and proteins: chemical modification, enrichment via solid-phase reversible binding, and analysis by mass spectrometry
    Florian Thaler
    Biology Department, Discovery Research Oncology, Pharmacia Corporation, Viale Pasteur 10, 20014 Nerviano, Italy
    Anal Bioanal Chem 376:366-73. 2003
    beta-Elimination of the phosphate group on phosphoserine and phosphothreonine residues and addition of an alkyldithiol is a useful tool for analysis of the phosphorylation states of proteins and peptides...
  93. pmc A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation
    Morten Frodin
    Department of Clinical Biochemistry, Glostrup Hospital, DK 2600 Glostrup, Denmark
    EMBO J 21:5396-407. 2002
    ..Here, we discover a pocket in the kinase domain of PDK1 that recognizes the phosphoserine/phosphothreonine in the hydrophobic motif by identifying two oppositely positioned arginine and lysine ..
  94. pmc Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B
    Michael P Scheid
    Department of Experimental Therapeutics, University Health Network Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
    Mol Cell Biol 22:6247-60. 2002
    ....
  95. ncbi Characterization of agonist stimulation of cAMP-dependent protein kinase and G protein-coupled receptor kinase phosphorylation of the beta2-adrenergic receptor using phosphoserine-specific antibodies
    Tuan M Tran
    The University of Texas, Houston, Medical School, Houston, TX 77225, USA
    Mol Pharmacol 65:196-206. 2004
    ..Ser356 of the human beta2AR overexpressed in human embryonic kidney (HEK) 293 cells were characterized using phosphoserine-specific antibodies...
  96. pmc RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea
    Jing Yuan
    Department of Molecular Biophysics and Biochemistry and Chemistry, Yale University, New Haven, CT 06520 8114, USA
    Proc Natl Acad Sci U S A 103:18923-7. 2006
    ..for a different route present in these domains of life that requires the tRNA(Sec)-dependent conversion of O-phosphoserine (Sep) to Sec...
  97. pmc Biosynthesis of selenocysteine on its tRNA in eukaryotes
    Xue Ming Xu
    Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Biol 5:e4. 2007
    ..Conservation of the overall pathway of Sec biosynthesis suggests that this pathway is also active in other eukaryotes and archaea that synthesize selenoproteins...
  98. pmc Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells
    F Matsumura
    Department of Molecular Biology and Biochemistry, Rutgers University, Nelson Labs, Busch Campus, Piscataway, New Jersey 08855, USA
    J Cell Biol 140:119-29. 1998
    ....
  99. ncbi Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody
    D L Clemm
    Endocrine Research, Ligand Pharmaceuticals, Inc, San Diego, California 92121, USA
    Mol Endocrinol 14:52-65. 2000
    ..That Ser 294 lies within an inhibitory domain that mediates the unique repressive functions of PR-A raises the possibility that differential phosphorylation of Ser 294 is involved in the distinct functional properties of PR-A and PR-B...
  100. pmc Biosynthesis of phosphoserine in the Methanococcales
    Sunna Helgadóttir
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    J Bacteriol 189:575-82. 2007
    ..These methanogens charge tRNA(Cys) with l-phosphoserine, which is also an intermediate in the predicted pathways for serine and cystathionine biosynthesis...
  101. ncbi Chemical derivatization of phosphoserine and phosphothreonine containing peptides to increase sensitivity for MALDI-based analysis and for selectivity of MS/MS analysis
    Giorgio Arrigoni
    Department of Protein Technology, Lund University, Lund, Sweden
    Proteomics 6:757-66. 2006
    ..show an approach using a simple one-step beta-elimination/Michael addition reaction for the derivatization of phosphoserine and phosphothreonine...

Research Grants62

  1. Chemistry and biology of protein histidine phosphorylation
    Tom Muir; Fiscal Year: 2013
    ..While protein phosphorylation is known to occur on several amino acids, it is fair to say that phosphoserine, phosphothreonine and phosphotyrosine capture most of the attention in the literature, at least as it pertains ..
  2. Mechanisms and regulation of serine resolvases
    Phoebe A Rice; Fiscal Year: 2012
    ..It is known that they form phosphoserine intermediates, but the structures available to date do not show a fully assembled active site, making it ..
  3. Understanding and Eliminating Oncogenic EGFR Signaling in Malignant Glioma
    Alain Charest; Fiscal Year: 2013
    ..by fulfilling the following goals: 1) To determine and study network dynamics of phosphotyrosine and phosphoserine/threonine signaling events in GBM tumors from our mouse models using mass spectrometry...
  4. Mechanism of selenoprotein synthesis
    Marla Berry; Fiscal Year: 2009
    ..Selenocysteine is synthesized on a specialized tRNA, utilizing a phosphoserine precursor and selenophosphate as selenium donor...
  5. Signaling Crosstalk Controlled by a Mad Phosphoserine Code
    EDWARD V EIVERS; Fiscal Year: 2013
    ..We anticipate this proposal will contribute to the fields of development and cell signaling. In principle, by better understanding signaling processes, new strategies can be developed to protect and improve human health. ..
  6. Studies of Transfer RNA
    Dieter Soll; Fiscal Year: 2013
    ..While some ngeAAs have been genetically encoded (e.g., N-acetyl lysine, phosphoserine (Sep)), OTSs have not been established for a number of critical PTMs...
  7. The mechanism of selenium incorporation into selenocysteine in humans.
    Miljan Simonovic; Fiscal Year: 2013
    ..In the terminal reaction, selenocysteine-tRNA synthase (synthase) promotes the conversion of phosphoserine into selenocysteine in a reaction that requires selenophosphate...
  8. Phospho-Binding Ligands and Substrates of BRCA1
    Michael B Yaffe; Fiscal Year: 2013
    ..We previously identified the BRCT domains of BRCA1 as phosphoserine/threonine-binding domains, and this phospho-binding function has recently been shown to be critical for BRCA1&..
  9. Molecular mechanisms of yeast PAS kinase regulation and function.
    Julianne H Grose; Fiscal Year: 2012
    ..phosphorylation of the two yeast PAS kinase homologs, Psk1 and Psk2, has recently been detected using anti-phosphoserine antibodies...
  10. Protein Kinase Signaling and Cell Cycle Control
    Michael B Yaffe; Fiscal Year: 2013
    ..The long-term goal of our laboratory is to understand how specific protein kinase signaling pathways and phosphoserine/threonine-binding domains regulate cell cycle progression and the response to DNA damage...
  11. Aptamers to distinguish functional modification of target proteins by affinity pr
    STEVEN SULJAK; Fiscal Year: 2009
    ..establishing reliable APCE methods for a selected peptide domain, specific modified peptides that incorporate phosphoserine and/or phosphothreonine will be investigated as models of typical post-translational modifications...
  12. Paxillin as a Liaison between Extranuclear and Intranuclear Steroid Signaling
    Stephen R Hammes; Fiscal Year: 2013
    ..Once activated, Erk then regulates phosphorylation of paxillin on serine residues, at which point phosphoserine-paxillin travels to the nucleus and mediates AR- and Erk-dependent transcription...
  13. METAL DEPENDENT COMPLEXES IN BLOOD COAGULATION
    Barbara Furie; Fiscal Year: 2002
    ..fragment 1 bound to calcium ions and glycerophosphoserine and/or a short chain phospholipid bearing the phosphoserine head group...
  14. THE ROLE OF PIN1 IN MITOSIS WITH DESIGNED INHIBITORS
    FELICIA ETZKORN; Fiscal Year: 2002
    DESCRIPTION: Pin 1, a newly discovered regulator of mitosis signal transduction, is an enzyme that acts on phosphoserine-proline amides...
  15. MOLECULAR MECHANISM OF MATRIX PROTEIN PHOSPHORYLATION
    CHOU WU; Fiscal Year: 1993
    ..e., same kinase on different substrates) will be investigated. The preferential phosphoamino acid residue (phosphoserine or phosphothreonine) and the sequence specificity among the sites of the phosphorylated substrates will also ..
  16. Designed Inhibitors of Pin1 in Mitosis
    FELICIA ETZKORN; Fiscal Year: 2009
    Pin1 is an enzyme that catalyzes isomerization of phosphoserine/threonine-proline (pSer/Thr-Pro) amides...
  17. SPECIFICITY OF PROTEIN TYROSINE PHOSPHATASES
    Zhong Yin Zhang; Fiscal Year: 2001
    ..of the dual specificity phosphatases that can utilize substrates containing phosphotyrosine as well as phosphoserine/phosphothreonine...
  18. PHOSPHOPROTEIN INTERACTIONS WITH IRON AND OTHER METALS
    GEORGE TABORSKY; Fiscal Year: 1990
    ..to some of the relatively recently discovered mammalian polyphosphoproteins that contain phosvitin-like phosphoserine clusters and are associated with, for example, neural, dermal and dental tissue or cells...
  19. NEW INSIGHTS INTO ENZYME STRUCTURE/FUNCTION
    Evan Kantrowitz; Fiscal Year: 2001
    ..which the active site serine has been replaced in order to learn more about phosphoester hydrolysis without a phosphoserine intermediate, and (vi) continue to elucidate the contribution of individual amino acids and the metals towards ..
  20. PROTEIN INHIBITORS OF CALCIUM PHOSPHATE PRECIPITATION
    David Schlesinger; Fiscal Year: 1980
    ..of human origin, we have been able to demonstrate that the NH2-terminal hexapeptide, containing the two phosphoserine residues, contains the part of the sequence required for phase-transformation inhibition but not for ..
  21. Chlamydia trachomatis Inclusion Membrane Proteins
    Marci Scidmore; Fiscal Year: 2006
    ..14-3-3 proteins are dimeric phosphoserine binding proteins that regulate diverse signal transduction pathways through directed subcellular localization ..
  22. STRUCTURE/FUNCTION ANALYSIS OF MITOTIC REGULATORS
    JOSEPH NOEL; Fiscal Year: 2001
    ..in progress, confirms that optimal interaction with this basic cluster is achieved with phosphothreonine or phosphoserine containing substrates...
  23. Developmental & Immunoregulatory Effects of TNF
    Hugh McDevitt; Fiscal Year: 2004
    ..expression profiling on cDNA microarrays, and by specific immunoblotting with anti-phosphotyrosine and anti-phosphoserine. Paradoxically, TNFalpha treatment in the neonatal period leads to an earlier onset and increased incidence of ..
  24. The Role of TNF alpha and Apoptosis in Type 1 Diabetes
    Li Fen Lee; Fiscal Year: 2007
    ..expression profiling on cDNA microarrays and by specific immunoblotting with anti-phosphotyrosine and anti-phosphoserine. These studies will aid in understanding the molecular mechanism ofapoptosis and the role of TNF and anti-TNF ..
  25. ROLE OF VACCINIA VP11 PHOSPHOPROTEIN IN MORPHOGENESIS
    MICHAEL REDDY; Fiscal Year: 2000
    ..This proposal involves a focused study on an essential VV phosphoserine protein called VP11, which is encoded by the F17R gene...
  26. A NOVEL RECEPTOR: 14-3-3 AXIS REGULATES CELL FUNCTION
    Angel Lopez; Fiscal Year: 2006
    ..feature of this pathway is its initiation by receptor site-specific serine phosphorylation and recruitment of phosphoserine-binding adaptor protein 14-3-3. The specific aims of this proposal are to: 1...
  27. Hsp90 Mediates eNOS and Vascular Function
    Kirkwood Pritchard; Fiscal Year: 2005
    ..As the presence of phosphoserine on eNOS inversely correlates with O2 about this site may influence the function of eNOS by directing radical ..
  28. 14-3-3 PROTEIN FUNCTION IN CELL GROWTH AND MOTILITY
    ANTHONY MUSLIN; Fiscal Year: 2001
    ..The first will be to assess the ability of 14-3-3 to bind to specific phosphoserine motifs in vivo (through the use of mutant forms of the Raf-1 kinase)...
  29. REGULATION OF BAD & ITS ROLE IN DEVELOPMENT & NEOPLASIA
    Jiping Zha; Fiscal Year: 2003
    ..Instead, it remains sequestered by the cytosolic phosphoserine binding protein 14-3-3...
  30. STUDIES OF THE COMPONENTS OF HUMAN GASTRIC JUICE
    Jordan Tang; Fiscal Year: 1991
    ..recognition, (e) the roles of residues in the activation peptides of pepsinogen, and (f) the role of phosphoserine at residue 68...
  31. ISOLATION OF INSULIN RECEPTOR PHOSPHATASES
    Laurel Sweet; Fiscal Year: 1991
    ..the phosphatase will be examined comparing dephosphorylation of the insulin receptor to other phosphotyrosine, phosphoserine, and phosphothreonine-containing substrates...
  32. CONTROL OF APOPTOSIS SIGNAL-REGULATING KINASE (ASK) 1
    Haian Fu; Fiscal Year: 2003
    ..Our preliminary data indicate that ASK1 is specifically associated with 14 3 3, a family of phosphoserine binding proteins...
  33. Mass Spectrometry Mapping of IRS-1 Phosphorylation
    Michael MacCoss; Fiscal Year: 2003
    ..the location of serine phosphorylation sites of IRS-1 and ii) measure the site-specific changes in IRS-1 phosphoserine and phosphotyroserine levels between normal and insulin resistant conditions...
  34. Role of L-3-phosphoserine phosphatase in epidermal growth and survival
    David Owens; Fiscal Year: 2008
    unreadable] DESCRIPTION (provided by applicant): L-3-phosphoserine phosphatase (L3PSP) is a widely expressed member of the haloacid dehalogenase (HAD) superfamily and its enzyme activity is the irreversible and rate limiting step in L-..
  35. 2004 Growth Factor Signalling Gordon Conference
    Michael Yaffe; Fiscal Year: 2008
    ..abstract_text> ..
  36. Role of L-3 phosphoserine phosphatase in epidermal apoptosis and carcinogenesis
    MICHAEL BACHELOR; Fiscal Year: 2008
    unreadable] DESCRIPTION (provided by applicant): L-3-phosphoserine phosphatase (L3PSP) is a widely expressed member of the haloacid dehalogenase (HAD) superfamily and its enzyme activity is the irreversible and rate limiting step in L-..
  37. Phosphoserine Dependant Assembly of Signaling complexes
    Michael Yaffe; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): [unreadable] Phosphoserine/threonine-binding domains play critical roles in controlling multiple aspects of cell proliferation, including cell cycle progression and the cellular response ..
  38. NCRR Shared Instrumentation Grant (SIG) Program
    Michael Yaffe; Fiscal Year: 2005
    ..abstract_text> ..
  39. High-Throughput Assay Design:Polo Kinase Inhibitors(RMI)
    Michael Yaffe; Fiscal Year: 2004
    b>Phosphoserine/threonine-binding domains and protein kinases play critical roles in controlling multiple aspects of cell proliferation, including cell cycle progression and the cellular response to DNA damage...
  40. Identification of Molecular Pathways in Cancer Biology
    Michael Yaffe; Fiscal Year: 2005
    ..In this regard, phosphoSerine/Threonine (pSer/pThr)-binding domains such as 14-3-3 proteins, FHA domains, WW domains, and WD40 repeats are ..
  41. Advancing High Throughput Flow Cytometry
    Bruce S Edwards; Fiscal Year: 2010
    ....
  42. Prolyl Isomerase Pin 1 and Neurodegeneration
    Kun Ping Lu; Fiscal Year: 2009
    ..These studies should help elucidate the molecular mechanisms of AD and related disorders, and may also have novel implications for their therapies. ..
  43. Function and Regulation of Pin2/TRF1 and PinX1
    Kun Ping Lu; Fiscal Year: 2005
    ..These studies should yield new insights into telomere maintenance, cell growth control and oncogenesis, and may lead to the development of new anticancer therapy. ..
  44. STAT SIGNALING IN CHRONIC LYMPHOCYTIC LEUKEMIA
    David Frank; Fiscal Year: 2003
    ..As a result of these experiments, we plan to discern the role of STATs in the abnormal growth of CLL cells, and to identify targets for novel and specific therapies. ..
  45. MICROARRAY ANALYSIS OF HUMAN COCAINE ADDICTS
    DONALD KUHN; Fiscal Year: 2002
    ..Microarray-based scanning of the human genome has the potential to establish those genes or gene clusters that define the addictive phenotype, leading the way to proteomic-based studies of the bases of addiction. ..
  46. ANTITUMOR DRUG DNA AND DNA APTAMER COMPLEXES IN SOLUTION
    Dinshaw Patel; Fiscal Year: 2002
    ....
  47. SURFACTANT PROTEINS A AND D AND MYCOBACTERIA
    DENNIS VOELKER; Fiscal Year: 2004
    ....
  48. TRANSCRIPTIONAL CONTROL OF COLON CANCER THROUGH PPARY
    Bruce Spiegelman; Fiscal Year: 2004
    ..Since PPAR-gamma ligands are clinically available now, this work may provide a framework in which to contemplate new therapeutic approaches to colon cancer. ..
  49. REGULATION OF GENE EXPRESSION BY OXYGEN
    MARIA CZYZYK KRZESKA; Fiscal Year: 2005
    ..An understanding of the physiological role of pVhl in regulation of gene expression during hypoxia will provide much needed information on how O2-sensitive cells respond and adapt to hypoxia. ..
  50. Mussel-Inspired Protective Coatings
    JOHN WAITE; Fiscal Year: 2006
    ..All films will be evaluated by macro- and nanomechanical tests, and their resistance to a diverse range of biochemical, thermal, and mechanical insults will be examined. ..
  51. ROLE OF PROTEIN PHOSPHATASES IN INSULIN SECRETION
    Anjaneyulu Kowluru; Fiscal Year: 2004
    ....
  52. Molecular mechanisms of airway smooth muscle function
    Dale Tang; Fiscal Year: 2007
    ..The knowledge obtained from these studies may disclose new biological targets for the development of more effective pharmacological treatment of pulmonary diseases such as asthma. ..
  53. HISTONE ACETYLTRANSFERASE AND TRANSCRIPTIONAL REGULATION
    Min Hao Kuo; Fiscal Year: 2005
    ..Moreover, individual roles played by the ADA and SAGA complexes in HIS3 expression are to be investigated both genetically and biochemically. ..
  54. Initiation and Inhibition of Medial Artery Calcification
    Paul Price; Fiscal Year: 2008
    ..abstract_text> ..
  55. Archaeal gene recruitment by the Chlamydiales
    David Graham; Fiscal Year: 2008
    ..Results from these experiments will support future microbiological studies of the role of this system in chlamydial infection and survival in the host cell. [unreadable] [unreadable] [unreadable]..
  56. Integrin-Laminin Interactions in Endothelial Cells
    Annette Gonzalez; Fiscal Year: 2008
    ..abstract_text> ..
  57. PROTEIN-RNA RECOGNITION IN NEURODEGENERATIVE SYNDROMES
    Dinshaw Patel; Fiscal Year: 2008
    ..abstract_text> ..
  58. GSK3: Neural Modulators and Mood Disorders
    Xiaohua Li; Fiscal Year: 2009
    ..This research is expected to provide significant new insight into the pathophysiology and the treatment of mood disorders. ..
  59. Biomimetic Blades: Mincing with Less Mineral
    JOHN WAITE; Fiscal Year: 2005
    ..The chief health benefits of this research will be insights about lightweight replacement materials with superior hardness and abrasion resistance. ..
  60. Intracellular targeting of RNAs in virus infection
    Roger Beachy; Fiscal Year: 2004
    ..As the system being used involves a virus model system, the knowledge gained by this research may lead to novel therapies and transgenic approaches to control virus replication and disease. ..
  61. Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative Stress
    Stuart A Meyers; Fiscal Year: 2010
    ....