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| enterobactinSummarySummary: An iron-binding cyclic trimer of 2,3-dihydroxy-N-benzoyl-L-serine. It is produced by E COLI and other enteric bacteria. Top Publications
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A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin productionLaxminarayana R Devireddy
Case Comprehensive Cancer Center and Department of Pathology, Case Western Reserve University, Cleveland, OH 44106, USA
Cell 141:1006-17. 2010..24p3 is an iron-trafficking protein that binds iron through association with a bacterial siderophore, such as enterobactin, or a postulated mammalian siderophore...
Enterobactin: an archetype for microbial iron transportKenneth N Raymond
Department of Chemistry, University of California, Berkeley, CA 94720 1460, USA
Proc Natl Acad Sci U S A 100:3584-8. 2003..Siderophores are small iron chelators that facilitate cellular iron transport. The siderophore enterobactin is a triscatechol derivative of a cyclic triserine lactone...
Siderophore-mediated iron transport in Bacillus subtilis and Corynebacterium glutamicumEmily A Dertz
Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720 1460, USA
J Biol Inorg Chem 11:1087-97. 2006..Hexadentate bacillibactin is the siderophore of Bacillus subtilis and is structurally similar to the better known enterobactin of Gram-negative bacteria such as Escherichia coli...
Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating ironTrude H Flo
Institute for Systems Biology, Seattle, Washington 98103, USA
Nature 432:917-21. 2004..Our finding represents a new component of the innate immune system and the acute phase response to infection...
Effects of macromolecular crowding on the intrinsic catalytic efficiency and structure of enterobactin-specific isochorismate synthaseMing Jiang
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China
J Am Chem Soc 129:730-1. 2007
TolC is involved in enterobactin efflux across the outer membrane of Escherichia coliCorinna Bleuel
Institut fur Mikrobiologie, Martin Luther Universität Halle, Halle, Germany, Kurt Mothes Str 3, 06120 Halle, Germany
J Bacteriol 187:6701-7. 2005Escherichia coli excretes the catecholate siderophore enterobactin in response to iron deprivation...
Mucosal lipocalin 2 has pro-inflammatory and iron-sequestering effects in response to bacterial enterobactinMichael A Bachman
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
PLoS Pathog 5:e1000622. 2009..Lcn2 binds and sequesters the iron-scavenging siderophore enterobactin (Ent), preventing bacterial iron acquisition...
Directed evolution of aryl carrier proteins in the enterobactin synthetaseZhe Zhou
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:11621-6. 2007..specific recognition at distinct points in assembly lines, we have used the two-module Escherichia coli enterobactin synthetase as a model system...
Overexpression and purification of ferric enterobactin esterase from Escherichia coli. Demonstration of enzymatic hydrolysis of enterobactin and its iron complexT J Brickman
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri Columbia 65212
J Biol Chem 267:12350-5. 1992The Escherichia coli ferric enterobactin esterase gene (fes) was cloned into the vector pGEM3Z under the control of the T7 gene 10 promoter and overexpressed to approximately 15% of the total cellular protein...
Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenaseJ Liu
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
J Bacteriol 171:791-8. 1989..coli chromosome encoding part of a cluster of genes involved in the biosynthesis of the iron chelator enterobactin has been determined...
Identification and characterization of a novel outer membrane protein receptor FetA for ferric enterobactin transport in Vibrio anguillarum 775 (pJM1)Hiroaki Naka
Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
Biometals 25:125-33. 2012..775 (pJM1) of two chromosomal genes encoding outer membrane proteins that operate in the transport of ferric enterobactin. One of them is a novel receptor that we named FetA and the other is the already characterized FvtA that ..
The role of isochorismate hydroxymutase genes entC and menF in enterobactin and menaquinone biosynthesis in Escherichia coliC Dahm
Institut fur Pharmazeutische Biologie, Rheinische Friedrich Wilhelms Universitat, Nussallee 6, D 53115 Bonn, Germany
Biochim Biophys Acta 1425:377-86. 1998..4.99.6) enzyme. These enzymes catalyze the first step in enterobactin or menaquinone biosynthesis, respectively...
Identification and characterization of a new ferric enterobactin receptor, CfrB, in CampylobacterFuzhou Xu
Department of Animal Science, University of Tennessee, Knoxville, Tennessee 37996 4574, USA
J Bacteriol 192:4425-35. 2010The ferric enterobactin (FeEnt) receptor CfrA is present in the majority of Campylobacter jejuni isolates and is responsible for high-affinity iron acquisition...
Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismataseF Rusnak
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
Biochemistry 29:1425-35. 1990The Escherichia coli entB gene, coding for the enterobactin biosynthetic enzyme isochorismatase, has been subcloned into the multicopy plasmid pKK223-3 under the control of the tac promoter...
Involvement of enterobactin synthesis pathway in production of microcin H47María F Azpiroz
Sección de Fisiología y Genética Bacterianas, Facultad de Ciencias, Montevideo 11 400, Uruguay
Antimicrob Agents Chemother 48:1235-41. 2004..and iroD homologues, respectively, and mchS4 was found to promote the production of the catecholate siderophore enterobactin. The possible relationship between enterobactin synthesis and MccH47 production was studied...
Direct measurements of the outer membrane stage of ferric enterobactin transport: postuptake bindingSalete M Newton
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA
J Biol Chem 285:17488-97. 2010..We devised a methodology that exclusively observes OM transport and used it to study the uptake of ferric enterobactin (FeEnt) by Escherichia coli FepA...
The pathogen-associated iroA gene cluster mediates bacterial evasion of lipocalin 2Michael A Fischbach
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 103:16502-7. 2006..Secreted lipocalin 2 (Lcn2) sequesters the Escherichia coli siderophore enterobactin (Ent), preventing E. coli from acquiring iron and protecting mammals from infection by E. coli...
Evidence of ball-and-chain transport of ferric enterobactin through FepALi Ma
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA
J Biol Chem 282:397-406. 2007..The reactivity of the Cys residues changed, sometimes dramatically, during the transport of ferric enterobactin, the natural ligand of FepA...
Identification of the Vibrio cholerae enterobactin receptors VctA and IrgA: IrgA is not required for virulenceAlexandra R Mey
Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712 1095, USA
Infect Immun 70:3419-26. 2002..One siderophore that V. cholerae transports, but does not make, is enterobactin. Enterobactin transport requires TonB and is independent of the vibriobactin receptor ViuA...
Environmental factors influence the production of enterobactin, salmochelin, aerobactin, and yersiniabactin in Escherichia coli strain Nissle 1917Marianne Valdebenito
, , Auf der Morgenstelle 28, , Germany
Int J Med Microbiol 296:513-20. 2006The probiotic Escherichia coli strain Nissle 1917 produces four siderophores: the catecholates enterobactin and salmochelin, the hydroxamate aerobactin, and the mixed-type siderophore yersiniabactin...
Enzymatic tailoring of enterobactin alters membrane partitioning and iron acquisitionMinkui Luo
ACS Chem Biol 1:29-32. 2006b>Enterobactin (Ent), a prototypic bacterial siderophore, is modified by both the C-glucosyltransferase IroB and the macrolactone hydrolase IroE in pathogenic bacteria that contain the iroA cluster...
Microbial evasion of the immune system: structural modifications of enterobactin impair siderocalin recognitionRebecca J Abergel
Department of Chemistry, University of California, Berkeley, California 94720-1460, USA
J Am Chem Soc 128:10998-9. 2006The mammalian protein siderocalin binds and inactivates the ferric complex of the bacterial siderophore enterobactin with a Kd value similar to that of the bacterial receptor FepA...
Characterization of the Aspergillus nidulans transporters for the siderophores enterobactin and triacetylfusarinine CHubertus Haas
Department of Molecular Biology, University of Innsbruck, Austria
Biochem J 371:505-13. 2003..high affinity iron transport systems, showed that MIRA transports specifically the heterologous siderophore enterobactin and that MIRB transports exclusively the native siderophore triacetylfusarinine C...
Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntFA M Gehring
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
Biochemistry 37:2648-59. 1998The siderophore molecule enterobactin, a cyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)serine, is synthesized and secreted by Escherichia coli in response to iron starvation...
How pathogenic bacteria evade mammalian sabotage in the battle for ironMichael A Fischbach
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
Nat Chem Biol 2:132-8. 2006..b>Enterobactin, a siderophore produced by enteric bacteria, is surprisingly ineffective as an iron-scavenging agent for ..
Ferric enterobactin binding and utilization by Neisseria gonorrhoeaeS D Carson
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
J Bacteriol 181:2895-901. 1999..We now report that FetA functions as an enterobactin receptor. N...
In vitro characterization of IroB, a pathogen-associated C-glycosyltransferaseMichael A Fischbach
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 102:571-6. 2005Pathogenic strains of Escherichia coli and Salmonella enterica modify the tricatecholic siderophore enterobactin (Ent) by glucosylation of three aryl carbon atoms, a process controlled by the iroA locus [Hantke, K., Nicholson, G...
Export of the siderophore enterobactin in Escherichia coli: involvement of a 43 kDa membrane exporterJason L Furrer
Department of Molecular Microbiology and Immunology, University of Missouri Columbia, Columbia, MO, USA
Mol Microbiol 44:1225-34. 2002The enterobactin system for iron transport in Escherichia coli is well characterized with the exception of the mechanism of enterobactin secretion to the extracellular environment...
Binding of ferric enterobactin by the Escherichia coli periplasmic protein FepBC Sprencel
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA
J Bacteriol 182:5359-64. 2000The periplasmic protein FepB of Escherichia coli is a component of the ferric enterobactin transport system...
Occurrence of the Yersinia high-pathogenicity island and iron uptake systems in clinical isolates of Klebsiella pneumoniaeRyszard Koczura
Department of Microbiology, Institute of Experimental Biology, Adam Mickiewicz University, ul Fredry 10, Poznan 61 701, Poland
Microb Pathog 35:197-202. 2003..The isolates employed several iron uptake mechanisms, including production of enterobactin (100%) and aerobactin (50%)...
A gene of the major facilitator superfamily encodes a transporter for enterobactin (Enb1p) in Saccharomyces cerevisiaeP Heymann
Department of Microbiology and Biotechnology, University of Tubingen, Germany
Biometals 13:65-72. 2000While in fungi iron transport via hydroxamate siderophores has been amply proven, iron transport via enterobactin is largely unknown...
The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein productS K Armstrong
Department of Microbiology, School of Medicine, University of Missouri Columbia 65212
Mol Microbiol 3:757-66. 1989The nucleotide sequence of the Escherichia coli enterobactin biosynthesis gene entD has been determined...
Biosynthesis and IroC-dependent export of the siderophore salmochelin are essential for virulence of Salmonella enterica serovar TyphimuriumMarie Laure V Crouch
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
Mol Microbiol 67:971-83. 2008..to iron deprivation, Salmonella enterica serovar Typhimurium secretes two catecholate-type siderophores, enterobactin and its glucosylated derivative salmochelin...
Molecular characterization of the Escherichia coli enterobactin cistron entF and coupled expression of entF and the fes geneG S Pettis
J Bacteriol 169:4154-62. 1987The Escherichia coli entF gene, which encodes the serine-activating enzyme involved in enterobactin synthesis, has been localized to a 4.7-kilobase-pair DNA fragment inserted in the vector pBR328...
Specific roles of the iroBCDEN genes in virulence of an avian pathogenic Escherichia coli O78 strain and in production of salmochelinsMélissa Caza
INRS Institut Armand Frappier, 531 Boul des Prairies, Laval, Quebec, Canada H7V 1B7
Infect Immun 76:3539-49. 2008..Overall, efficient glucosylation (IroB), transport (IroC and IroN), and processing (IroD and IroE) of salmochelins are required for APEC virulence, although IroE appears to serve an ancillary role...
Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate productF Rusnak
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
Biochemistry 28:6827-35. 1989..ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli...
The siderocalin/enterobactin interaction: a link between mammalian immunity and bacterial iron transportRebecca J Abergel
Department of Chemistry, University of California, Berkeley, California 94720 1460, USA
J Am Chem Soc 130:11524-34. 2008The siderophore enterobactin (Ent) is produced by enteric bacteria to mediate iron uptake. Ent scavenges iron and is taken up by the bacteria as the highly stable ferric complex [Fe (III)(Ent)] (3-)...
The hotdog thioesterase EntH (YbdB) plays a role in vivo in optimal enterobactin biosynthesis by interacting with the ArCP domain of EntBDamien Leduc
LISM, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille, France
J Bacteriol 189:7112-26. 2007In response to iron limitation, the siderophore enterobactin is synthesized and secreted by Escherichia coli. Its biosynthesis is performed by a series of enzymes encoded by the Ent gene cluster...
Cloning and characterization of the ferric enterobactin receptor gene (pfeA) of Pseudomonas aeruginosaC R Dean
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
J Bacteriol 175:317-24. 1993Pseudomonas aeruginosa K407, a mutant lacking a high-affinity 80,000-molecular-weight ferric enterobactin receptor protein (80K protein), exhibited poor growth (small colonies) on iron-deficient succinate minimal medium containing ..
Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoterG S Pettis
Department of Microbiology, School of Medicine, University of Missouri Columbia 65212
J Biol Chem 263:18857-63. 1988The iron-controlled fepA and fes-entF transcripts from the Escherichia coli enterobactin gene complex are expressed divergently from a limited genetic region, thereby suggesting the existence of a single, possibly overlapping promoter ..
Utilization of enterobactin and other exogenous iron sources by Haemophilus influenzae, H. parainfluenzae and H. paraphrophilusP Williams
Department of Pharmaceutical Sciences, University of Nottingham, UK
J Gen Microbiol 136:2343-50. 1990..However, the phenolate siderophore enterobactin supplied iron to both H. parainfluenzae and H...
Anaerobic biosynthesis of enterobactin Escherichia coli: regulation of entC gene expression and evidence against its involvement in menaquinone (vitamin K2) biosynthesisO Kwon
Department of Biological Sciences, Northern Illinois University, De Kalb, 60115, USA
J Bacteriol 178:3252-9. 1996In Escherichia coli, isochorismate is a common precursor for the biosynthesis of the siderophore enterobactin and menaquinone (vitamin K2)...
Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheineF Rusnak
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
Biochemistry 30:2916-27. 1991The sequence of the entF gene which codes for the serine activating enzyme in enterobactin biosynthesis is reported...
Interleukin-8 secretion in response to aferric enterobactin is potentiated by siderocalinAaron L Nelson
Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104 6076, USA
Infect Immun 75:3160-8. 2007..We hypothesized that the catecholate siderophore enterobactin, produced by Enterobacteriaceae, serves as a proinflammatory signal for respiratory epithelial cells...
Suppression of linear side products by macromolecular crowding in nonribosomal enterobactin biosynthesisZu Feng Guo
Department of Chemistry, Center for Cancer Research, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China
Org Lett 10:649-52. 2008Nonribosomal enterobactin synthetase of Escherichia coli was found to prematurely release a large amount of linear precursors in an in vitro reconstitution...
The growth response of Escherichia coli to neurotransmitters and related catecholamine drugs requires a functional enterobactin biosynthesis and uptake systemClaire L Burton
Institute of Infections and Immunity. Institute of Cell Signalling, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom
Infect Immun 70:5913-23. 2002..coli growth by NE in SAPI+serum may involve the catecholate siderophore, enterobactin, a cyclic triester of 2,3-dihydroxybenzoylserine. Consistent with this hypothesis, E...
Involvement of enterobactin in norepinephrine-mediated iron supply from transferrin to enterohaemorrhagic Escherichia coliPrimrose P E Freestone
Department of Microbiology and Immunology, University of Leicester, Leicester, UK
FEMS Microbiol Lett 222:39-43. 2003..Mutation of genes for enterobactin synthesis and uptake revealed an absolute requirement for enterobactin in norepinephrine-stimulated growth of ..
Genetic organization of multiple fep genes encoding ferric enterobactin transport functions in Escherichia coliB A Ozenberger
J Bacteriol 169:3638-46. 1987Three genes were shown to provide functions specific for ferric enterobactin transport in Escherichia coli: fepA encoded the outer membrane receptor, fepB produced a periplasmic protein, and the fepC product was presumably a component of ..
Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoateA M Gehring
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
Biochemistry 36:8495-503. 1997In Escherichia coli, the siderophore molecule enterobactin is synthesized in response to iron deprivation by formation of an amide bond between 2,3-dihydroxybenzoate (2,3-DHB) and l-serine and formation of ester linkages between three ..
Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entAM S Nahlik
Department of Microbiology, School of Medicine, University of Missouri Columbia 65212
J Bacteriol 171:784-90. 1989The nucleotide sequence of a 2,137-base-pair DNA fragment expressing enterobactin biosynthesis functions defined the molecular boundaries and translational products of the entB and entA genes and identified a closely linked downstream ..
Promoter and operator determinants for fur-mediated iron regulation in the bidirectional fepA-fes control region of the Escherichia coli enterobactin gene systemM D Hunt
Department of Molecular Microbiology and Immunology, University of Missouri, School of Medicine, Columbia 65212
J Bacteriol 176:3944-55. 1994The fepA-entD and fes-entF operons in the enterobactin synthesis and transport system are divergently transcribed from overlapping promoters, and both are inhibited by the Fur repressor protein under iron-replete conditions...
FepA with globular domain deletions lacks activityHema L Vakharia
School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234, USA
J Bacteriol 184:5508-12. 2002....
Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917Lubomir Grozdanov
Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, Wuerzburg, Germany
J Bacteriol 186:5432-41. 2004..coli strain Nissle 1917...
IroN functions as a siderophore receptor and is a urovirulence factor in an extraintestinal pathogenic isolate of Escherichia coliThomas A Russo
Department of Medicine, University of Buffalo, New York 14214, USA
Infect Immun 70:7156-60. 2002..In this study experimental evidence demonstrating that IroN mediates utilization of the siderophore enterobactin was obtained, thereby establishing IroN as a catecholate siderophore receptor...
The Yersinia high-pathogenicity island and iron-uptake systems in clinical isolates of Escherichia coliRyszard Koczura
Department of Microbiology, Institute of Experimental Biology, Adam Mickiewicz University, 61 701 Poznan, Poland
J Med Microbiol 52:637-42. 2003..The isolates employed several iron-uptake mechanisms, including production of enterobactin (86 %) and aerobactin (71 %)...
Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coliRanjan Chakraborty
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA
Biometals 16:507-18. 2003..normal transport as well, however, the majority showed moderate to severe defective transport with ferric enterobactin. The results therefore show the validity of the hypothesis that the simultaneous sequence alignment will select ..
Linkage between catecholate siderophores and the multicopper oxidase CueO in Escherichia coliGregor Grass
Department of Soil, Water, and Environmental Science, University of Arizona, Shantz Bldg 38, Rm 424, Tucson, AZ 85721, USA
J Bacteriol 186:5826-33. 2004..In this report we provide evidence that CueO protects E. coli cells by oxidizing enterobactin, the catechol iron siderophore of E. coli, in the presence of copper...
Genome-wide screen for genes with effects on distinct iron uptake activities in Saccharomyces cerevisiaeEmmanuel Lesuisse
Laboratoire d Ingéniérie des Protéines et Contrôle Métabolique, Département de Biologie des Génomes, Institut Jacques Monod, Unité Mixte de Recherche 7592 CNRS Universités Paris 6 and 7, France
Genetics 169:107-22. 2005..In the first analysis, siderophores grouped together with the exception of enterobactin, which grouped with iron salts, suggesting a reductive pathway of iron uptake for this siderophore...
FepA- and TonB-dependent bacteriophage H8: receptor binding and genomic sequenceWolfgang Rabsch
Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, OK 73019, USA
J Bacteriol 189:5658-74. 2007..Ferric enterobactin inhibited H8 binding to E...
Insight into siderophore-carrying peptide biosynthesis: enterobactin is a precursor for microcin E492 posttranslational modificationGaëlle Vassiliadis
Chimie et Biochimie des Substances Naturelles, CNRS Muséum National d Histoire Naturelle, UMR 5154, CP 54, 57 rue Cuvier, 75005, Paris, France
Antimicrob Agents Chemother 51:3546-53. 2007..linear trimer of N-(2,3-dihydroxybenzoyl)-l-serine, a catecholate siderophore related to salmochelins and enterobactin. We show here that repression of enterobactin biosynthesis inhibits the acquisition of microcin E492 ..
Identification and characterization of a novel ABC iron transport system, fit, in Escherichia coliZhiming Ouyang
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA
Infect Immun 74:6949-56. 2006..Mutations in fit did not affect bacterial growth in iron-restricted media. Using a growth promotion test, it was found that fit was not able to transport enterobactin, ferrichrome, transferrin, and lactoferrin in E. coli.
Bromoenterobactins as potent inhibitors of a pathogen-associated, siderophore-modifying C-glycosyltransferaseHening Lin
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
J Am Chem Soc 128:9324-5. 2006IroB is a C-glycosyltransferase encoded in the iroA cluster. C-Glucosylation of the bacterial siderophore enterobactin by IroB is a strategy some pathogenic bacteria use to evade the host's innate immunity mediated by lipocalin 2 (Lcn2)...
Studies on siderophore production of different diarrhoeagenic Escherichia coliSujata Bhattacharya
Department of Microbiology, RG Kar Medical College, Kolkata 700004
J Indian Med Assoc 105:110, 112, 114-8. 2007..None of the enterotoxigenic E coli and non-pathogenic strains produced aerobactin...
Reactivation of the vanchrobactin siderophore system of Vibrio anguillarum by removal of a chromosomal insertion sequence originated in plasmid pJM1 encoding the anguibactin siderophore systemHiroaki Naka
Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
Environ Microbiol 10:265-77. 2008..Our results underscore the importance of the anguibactin system in the survival of V. anguillarum 775 under conditions of iron limitation...
Bacillibactin-mediated iron transport in Bacillus subtilisEmily A Dertz
Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA
J Am Chem Soc 128:22-3. 2006..The hexadentate triscatecholamide bacillibactin delivers iron to Bacillus subtilis and is structurally similar to enterobactin, although in a more oblate conformation. B...
Cooperative uptake of microcin E492 by receptors FepA, Fiu, and Cir and inhibition by the siderophore enterochelin and its dimeric and trimeric hydrolysis productsErwin Strahsburger
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
Antimicrob Agents Chemother 49:3083-6. 2005..No TonB-mediated interaction with the ferric catecholate receptors is needed for microcin to exert action at the cytoplasmic membrane. Microcin E492 uptake by the receptors is inhibited by the dimer and trimer of dihydroxybenzoylserine...
The detection of salmochelin and yersiniabactin in uropathogenic Escherichia coli strains by a novel hydrolysis-fluorescence-detection (HFD) methodMarianne Valdebenito
, , Germany
Int J Med Microbiol 295:99-107. 2005Escherichia coli strains produce a variety of structurally different siderophores of which enterobactin, aerobactin and yersiniabactin have been reported earlier to occur in strains of extraintestinal infections. In uropathogenic E...
The Pseudomonas aeruginosa pirA gene encodes a second receptor for ferrienterobactin and synthetic catecholate analoguesBart Ghysels
Department of Molecular and Cellular Interactions, Laboratory of Microbial Interactions, Flanders Interuniversity Institute of Biotechnology VIB6, Vrije Universiteit Brussel, Brussels, Belgium
FEMS Microbiol Lett 246:167-74. 2005..aeruginosa PAO1 genome. However, except for a few (pyoverdine, pyochelin, enterobactin), the vast majority of P. aeruginosa siderophore receptor genes still remain to be characterized...
Enterohaemorrhagic Escherichia coli O157 and non-O157 serovars differ in their mechanisms for iron supplyAndreas U Kresse
Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, D 38855 Wernigerode, Germany
Int J Med Microbiol 297:9-15. 2007..As expected, all the strains produced enterobactin, but the prevalence of other factors varied among the serovars tested...
Production by Escherichia coli isolates of siderophore and other virulence factors and their pathogenic role in a cutaneous infection modelM Demir
Pamukkale University, Medical School, Department of Microbiology, Denizli, Turkey
Clin Microbiol Infect 10:1011-4. 2004..samples (n = 51) were examined for the presence of virulence factors, including siderophores (aerobactin and enterobactin). The proportion of aerobactin producers was significantly higher in UTI (69.4%; p 0...
Quantification of known components of the Escherichia coli TonB energy transduction system: TonB, ExbB, ExbD and FepAPenelope I Higgs
School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4234, USA
Mol Microbiol 44:271-81. 2002..Differences in the method of iron limitation also resulted in significant differences in cell size, skewing the per cell copy numbers for all proteins...
Genetic organization of the yersiniabactin biosynthetic region and construction of avirulent mutants in Yersinia pestisS W Bearden
Department of Microbiology and Immunology, University of Kentucky, Lexington 40536 0084, USA
Infect Immun 65:1659-68. 1997..Finally, Y. pestis strains with mutations in either the psn or irp2 gene were avirulent in mice when inoculated subcutaneously...
Exchangeability of N termini in the ligand-gated porins of Escherichia coliD C Scott
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA
J Biol Chem 276:13025-33. 2001..deleted the N termini of two such siderophore receptors, FepA and FhuA, which recognize and transport ferric enterobactin and ferrichrome, respectively...
Physical and genetic characterization of cloned enterobactin genomic sequences from Escherichia coli K-12T P Fleming
Gene 34:47-54. 1985We have cloned genes responsible for enterobactin synthesis (entD) and transport (fepA,fes) from Escherichia coli K-12. Relevant recombinant plasmids enabled EntD- and transport-defective mutants to grow on iron-limiting medium...
Aromatic components of two ferric enterobactin binding sites in Escherichia coli FepAZ Cao
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA
Mol Microbiol 37:1306-17. 2000Ferric enterobactin is a catecholate siderophore that binds with high affinity (Kd approximately 10-10 M) to the Escherichia coli outer membrane protein FepA...
Escherichia coli periplasmic protein FepB binds ferrienterobactinD L Stephens
Department of Microbiology, University of Texas at Austin 78712 1095, USA
Microbiology 141:1647-54. 1995..FepB is a periplasmic protein required for uptake of iron complexed to its endogenously-synthesized siderophore enterobactin (Ent)...
Isolation and characterization of Bacillus subtilis genes involved in siderophore biosynthesis: relationship between B. subtilis sfpo and Escherichia coli entD genesT H Grossman
Department of Microbial Genetics and Biochemistry, Lederle Laboratories, American Cyanamid Company, Pearl River, New York 10965
J Bacteriol 175:6203-11. 1993..2,3-dihydroxybenzoyl glycine, which is similar to the precursor of the Escherichia coli siderophore enterobactin. We isolated two sets of B. subtilis DNA sequences that complemented the mutations of several E...
Enterochelin acquisition in Campylobacter coli: characterization of components of a binding-protein-dependent transport systemP T Richardson
Institute of Food Research, Reading Laboratory, UK
Microbiology 141:3181-91. 1995..This is the first molecular characterization of a PBT system in Campylobacter species...
A Bordetella pertussis fepA homologue required for utilization of exogenous ferric enterobactinB Beall
Childhood and Respiratory Diseases Branch, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
Microbiology 141:3193-205. 1995The bfeA (Bordetella ferric enterobactin) receptor gene was cloned from a Bordetella pertussis chromosomal library by using a screen in Escherichia coli to detect iron-repressed genes encoding exported proteins translationally fused to ..
A multifunctional ATP-binding cassette transporter system from Vibrio cholerae transports vibriobactin and enterobactinE E Wyckoff
Department of Molecular Genetics and Microbiology, and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712 1095, USA
J Bacteriol 181:7588-96. 1999..This suggests that ViuP is a membrane lipoprotein. The ViuPDGC system transports both vibriobactin and enterobactin in Escherichia coli. In the same assay, the E...
Disruption of tonB in Bordetella bronchiseptica and Bordetella pertussis prevents utilization of ferric siderophores, haemin and haemoglobin as iron sourcesM L Nicholson
Centers for Disease Control and Prevention, Respiratory Diseases Branch, Atlanta, GA 30333, USA
Microbiology 145:2453-61. 1999..bronchiseptica resulted in inability to grow in iron-limiting media, and inability to utilize alcaligin, enterobactin, ferrichrome, desferroxamine B, haemin and haemoglobin...
Role of enterobactin and intracellular iron in cell lethality during near-UV irradiation in Escherichia coliJ Hoerter
Department of Biological Sciences, Ferris State University, Big Rapids, MI 49307 2225, USA
Photochem Photobiol 64:537-41. 1996In Escherichia coli, fur mutants that constitutively express their native iron chelating agent, enterobactin, are significantly more sensitive to near-UV radiation (NUV) than wild type, An entA mutant, which is incapable of synthesizing ..
Protonmotive force, ExbB and ligand-bound FepA drive conformational changes in TonBR A Larsen
Department of Microbiology, Washington State University, Pullman 99164 4233, USA
Mol Microbiol 31:1809-24. 1999....
An iron-regulated outer-membrane protein specific to Bordetella bronchiseptica and homologous to ferric siderophore receptorsB Beall
Childhood and Respiratory Diseases Branch, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
Microbiology 143:135-45. 1997..homologous to Cir of Escherichia coli, IrgA of Vibrio cholerae and to three previously characterized ferric enterobactin receptors. DNA hybridization results indicated that bfrA was not present in other Bordetella species...
Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in Escherichia coli. Homology among outer membrane receptors that interact with TonBM D Lundrigan
J Biol Chem 261:10797-801. 1986..The function of these three regions remains speculative...
A factor produced by Escherichia coli K-12 inhibits the growth of E. coli mutants defective in the cytochrome bd quinol oxidase complex: enterochelin rediscoveredG M Cook
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, UK
Microbiology 144:3297-308. 1998..Cyd- mutants overproduce siderophores, presumably reflecting intracellular iron deprivation...
Fe(III) coordination properties of two new saccharide-based enterobactin analogues: methyl 2,3,4-tris-O-[N-[2,3-di(hydroxy)benzoyl-glycyl]-aminopropyl]-alpha-D-glucopyranoside and methyl 2,3,4-tris-O-[N-[2,3-di-(hydroxy)-benzoyl]-aminopropyl]-alpha-D-glucS Dhungana
Department of Chemistry, Duke University, Box 90346 Durham, North Carolina 27708-0346, USA
Inorg Chem 40:7079-86. 2001The synthesis of two saccharide-based enterobactin analogues, methyl 2,3,4-tris-O[-N[2,3-di(hydroxy)benzoyl-glycyl]-aminopropyl]-alpha-D-glucopyranoside (H(6)L(A)) and methyl 2,3,4-tris-O-[N-[2,3-di(hydroxy)benzoyl]-aminopropyl]-alpha-D-..
Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coliC M Shea
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia 65212
Mol Microbiol 5:1415-28. 1991..Three new loci were identified. One of these, P43, encodes a membrane protein that is not essential for ferric enterobactin transport...
Mutations in the Escherichia coli receptor FepA reveal residues involved in ligand binding and transportT J Barnard
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, Missouri 65212, USA
Mol Microbiol 41:527-36. 2001FepA is the Escherichia coli outer membrane receptor for ferric enterobactin, colicin D and colicin B. The transport processes through FepA are energy-dependent, relying on the periplasmic protein TonB to interact with FepA...
Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepBM F Elkins
Department of Microbiology, University of Texas, Austin 78712 1095
J Bacteriol 171:5443-51. 1989..Processing of proFepB remains unclear, but the appearance of the three smaller members of the FepB family required the action of leader peptidase and the presence of the entire fepB gene...
Dihydroxybenzoylserine--a siderophore for E. coliK Hantke
Mikrobiologie II, Univ Tübingen, F R G
FEMS Microbiol Lett 55:5-8. 1990..Transport of Fe3(+)-dihydroxybenzoylserine across the cytoplasmic membrane was only dependent on genes from the fep region. In addition, it was shown that dihydroxybenzoate was taken up via Fiu and Cir and less efficiently by FepA...
Iron acquisition functions expressed by the human pathogen Acinetobacter baumanniiDaniel L Zimbler
Department of Microbiology, Miami University, Oxford, OH 45056, USA
Biometals 22:23-32. 2009..This system restored enterobactin biosynthesis in an E. coli ExbBD deficient strain but not when introduced into a TonB mutant...
Genetic organization of an Acinetobacter baumannii chromosomal region harbouring genes related to siderophore biosynthesis and transportCaleb W Dorsey
Department of Microbiology, Miami University, Oxford, OH 45056, USA
Microbiology 149:1227-38. 2003..Complementation assays with enterobactin-deficient Escherichia coli strains led to the isolation of a cosmid clone containing A...
Utilization of heterologous siderophores enhances levels of iron available to Pseudomonas putida in the rhizosphereJ E Loper
Agricultural Research Service, U S Department of Agriculture, and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97330, USA
Appl Environ Microbiol 65:5357-63. 1999..In culture, strain N1R Pvd(-) also utilized ferric complexes of the siderophores enterobactin and aerobactin as sources of iron...
Siderophore-mediated iron acquisition systems in Bacillus cereus: Identification of receptors for anthrax virulence-associated petrobactin Anna M Zawadzka
Department of Chemistry, University of California, Berkeley, 94720 1460, USA
Biochemistry 48:3645-57. 2009..with bioinformatics sequence comparison identify SBPs from five putative transporters specific for BB and enterobactin (FeuA), 3,4-DHB and PB (FatB), PB (FpuA), schizokinen (YfiY), and desferrioxamine and ferrichrome (YxeB)...
A new enzyme superfamily - the phosphopantetheinyl transferasesR H Lambalot
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Chem Biol 3:923-36. 1996..Surprisingly, initial searches of sequence databases did not reveal any proteins with significant peptide sequence similarity with ACPS...
Molecular, antigenic, and functional characteristics of ferric enterobactin receptor CfrA in Campylobacter jejuniXimin Zeng
Department of Animal Science, The University of Tennessee, Knoxville, Tennessee 37996 4574, USA
Infect Immun 77:5437-48. 2009The ferric enterobactin receptor CfrA not only is responsible for high-affinity iron acquisition in Campylobacter jejuni but also is essential for C. jejuni colonization in animal intestines...
Norepinephrine mediates acquisition of transferrin-iron in Bordetella bronchisepticaMark T Anderson
Department of Microbiology, University of Minnesota, Minneapolis, MN 55455 0312, USA
J Bacteriol 190:3940-7. 2008..Although norepinephrine is known to induce transcription of the Bordetella bfeA enterobactin catechol xenosiderophore receptor gene, neither a bfeA mutant nor a bfeR regulator mutant was defective in ..
Expression and characterization of an iron-regulated hemin-binding protein, HbpA, from Leptospira interrogans serovar LaiSwapna Asuthkar
School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad 500046, India
Infect Immun 75:4582-91. 2007In an earlier study, based on the ferric enterobactin receptor FepA of Escherichia coli, we identified and modeled a TonB-dependent outer membrane receptor protein (LB191) from the genome of Leptospira interrogans serovar Lai...
The fbpABC operon is required for Ton-independent utilization of xenosiderophores by Neisseria gonorrhoeae strain FA19Heather R Strange
Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia 23298, USA
Infect Immun 79:267-78. 2011..Among a panel of diverse siderophores, only the catecholate xenosiderophores enterobactin and salmochelin promoted growth of gonococcal strain FA19...
Signal transduction and transcriptional and posttranscriptional control of iron-regulated genes in bacteriaJ H Crosa
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201, USA
Microbiol Mol Biol Rev 61:319-36. 1997..genes as reported for the ferric dicitrate system in Escherichia coli; the pyochelin, pyoverdin, and enterobactin systems in Pseudomonas species; the irgB system in Vibrio cholerae; and the plasmid-mediated anguibactin system ..
Research Grants
- IRON ACQUISITION IN BORDETELLA PERTUSSISSANDRA KAY ARMSTRONG; Fiscal Year: 2010..pertussis and B. bronchiseptica produce and utilize the siderophore alcaligin, and can also use the enterobactin siderophore and host heme compounds...
- Role of the Mammalian Siderophore in Cellular Iron Metabolism and ErythropoiesisLaxminarayana R Devireddy; Fiscal Year: 2010..Instead a small molecule, a siderophore, such as enterobactin an E.coli gene product, mediates iron- binding...
- Role of the Mammalian Siderophore in Cellular Iron Metabolism and ErythropoiesisLAXMINARAYANA DEVIREDDY; Fiscal Year: 2009..Instead a small molecule, a siderophore, such as enterobactin an E.coli gene product, mediates iron- binding...
- Iron acqisition in Bordetella pertussisSANDRA ARMSTRONG; Fiscal Year: 2007..utilization of host heme compounds as well as certain non-native siderophores, including the potent siderophore enterobactin. These three iron-retrieval systems have distinct positive transcriptional regulators that respond to the ..
- INTERACTION OF SPIN LABELS WITH TRANSITION METALSGARETH EATON; Fiscal Year: 2007..cluster in electron transfer flavoprotein ubiquinone oxidoreductase (ETF-QO) and the non-heme iron in iron enterobactin (FeEnt) bound to iron protein A (FepA)...
- DEVELOPMENT OF BIOMEDICAL ESR INSTRUMENTATIONJames Hyde; Fiscal Year: 2003..label in super-cooled sec-butylbenzene, hemoglobin labeled at beta93 in glycerol-water mixtures, and the ferric enterobactin receptor, Fep-A. The SR method is validated and compared with more conventional methodology...
- Acinetobactin and C-Glucosylated Enterobactin SiderophoresChristopher Walsh; Fiscal Year: 2007The first focus is on enterobacterial strains that turn on biosynthetic genes for the siderophore enterobactin in microenvironments where iron is limiting, including vertebrate hosts...
- Acinetobactin and C-Glucosylated Enterobactin SiderophoresChristopher T Walsh; Fiscal Year: 2010The first focus is on enterobacterial strains that turn on biosynthetic genes for the siderophore enterobactin in microenvironments where iron is limiting, including vertebrate hosts...
- SYNTHETIC STUDIES ON COMPLEX NATURAL PRODUCTSYoshito Kishi; Fiscal Year: 1999..Ni(II)/Cr(II)-Mediated Coupling Reaction: Asymmetric Process, 6. Tripodal Ligand Chemistry: Enterobactin analogs and others, and 7. Total Synthesis of Batrachotoxin and Aranotin...
- ENZYMATIC REACTION MECHANISMSChristopher Walsh; Fiscal Year: 2003..coli iron chelator enterobactin. In particular the EntF enzyme has four domains (condensation, adenylation, peptidyl carrier protein and ..
- INTERACTION OF SPIN LABELS WITH TRANSITION METALSGARETH EATON; Fiscal Year: 2004..of the iron transport protein, iron protein A, did not define the binding site of the iron siderophore, iron enterobactin. We propose to use our pulse techniques to determine the location of the iron binding site...
- Microbial siderophore-specific innate immune responsesRoland Strong; Fiscal Year: 2007..high-affinity ligands for this protein: bacterial phenolate-type ferric siderophores, such as enterochelin (aka enterobactin; KD = 0.4 nanomolar) and the mixed-type carboxymycobactin ferric siderophores...
- BRUKER ELEXSYS EPR SPECTROMETERJimmy Feix; Fiscal Year: 2001..projects that will benefit include (1) characterization of ligand-induced conformational changes in the ferric enterobactin receptor as studied by site-directed spin labeling, (2) studies of oxidation/nitration by nitric oxide, (3) ..
- SITE DIRECTED SPIN LABELING OF FEPAJimmy Feix; Fiscal Year: 2002..coli and many other gram-negative bacteria via the binding and transport of its cognate ligand, ferric enterobactin (FeEnt)...
- V. cholerae iron transport mechanisms and pathogenesisSHELLEY PAYNE; Fiscal Year: 2006..cholerae, enterobactin and schizokinen...
