azaserine

Summary

Summary: Antibiotic substance produced by various Streptomyces species. It is an inhibitor of enzymatic activities that involve glutamine and is used as an antineoplastic and immunosuppressive agent.

Top Publications

  1. ncbi Purification and characterization of NADH-dependent glutamate synthase from the silkworm fat body (Bombyx mori)
    C Hirayama
    National Institute of Sericultural and Entomological Science, Ibaraki, Japan
    Insect Biochem Mol Biol 28:473-82. 1998
  2. ncbi The hexosamine biosynthesis pathway regulates insulin secretion via protein glycosylation in mouse islets
    Sakeneh Zraika
    Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Parkville, VIC 3050, Australia
    Arch Biochem Biophys 405:275-9. 2002
  3. doi The hexosamine biosynthesis inhibitor azaserine prevents endothelial inflammation and dysfunction under hyperglycemic condition through antioxidant effects
    Angana Gupta Rajapakse
    Division of Physiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland
    Am J Physiol Heart Circ Physiol 296:H815-22. 2009
  4. ncbi Overexpression of cholecystokinin receptors in azaserine-induced neoplasms of the rat pancreas
    R H Bell
    Department of Pathology, Dartmouth Medical School, Hanover, New Hampshire 03756
    Cancer Res 52:3295-9. 1992
  5. ncbi Cellular autophagic capacity changes during azaserine-induced tumour progression in the rat pancreas. Up-regulation in all premalignant stages and down-regulation with loss of cycloheximide sensitivity of segregation along with malignant transformation
    Szilveszter Tóth
    Department of General Zoology, Lorand Eotvos University, Budapest, Pf 120, 1518, Hungary
    Cell Tissue Res 309:409-16. 2002
  6. ncbi Changes in cellular autophagic capacity during azaserine-initiated pancreatic carcinogenesis
    S Toth
    Department of General Zoology, Eotvos Lorand University, Budapest, Hungary
    Acta Biol Hung 52:393-401. 2001
  7. ncbi Overexpression of messenger RNA for cholecystokinin-A receptor and novel expression of messenger RNA for gastrin (cholecystokinin-B) receptor in azaserine-induced rat pancreatic carcinoma
    W Zhou
    Department of Surgery, University of Cincinnati College of Medicine, OH
    Carcinogenesis 14:2189-92. 1993
  8. pmc Inhibition of glycosaminoglycan synthesis and protein glycosylation with WAS-406 and azaserine result in reduced islet amyloid formation in vitro
    Rebecca L Hull
    Division of Metabolism, Endocrinology, and Nutrition, Veterans Affairs Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA
    Am J Physiol Cell Physiol 293:C1586-93. 2007
  9. ncbi The cytotoxicity of DNA carboxymethylation and methylation by the model carboxymethylating agent azaserine in human cells
    M O'Driscoll
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, London, UK
    Carcinogenesis 20:1855-62. 1999
  10. ncbi Cellular autophagic capacity is highly increased in azaserine-induced premalignant atypical acinar nodule cells
    G Réz
    Department of General Zoology, Lorand Eotvos University, pf 330, H 1445 Budapest, Hungary grez2cerberus elte hu
    Carcinogenesis 20:1893-8. 1999

Research Grants

Scientific Experts

  • G Wu
  • S M Downs
  • Fuller Bazer
  • Rebekka Vibjerg Jensen
  • Y Yener
  • Satoshi Yoshida
  • Jörgen Persson
  • D M Harris
  • Marie Pantaleon
  • Lalit P Singh
  • C Hirayama
  • L Wollweber
  • Kimmo Makinen
  • Nirupama Mallick
  • G S Bondy
  • Marko J Appel
  • L R James
  • R L Hull
  • S Esposito
  • Gregory Stephanopoulos
  • Matthew Wallig
  • Daniel Longnecker
  • Edwin Cadman
  • Bill Roebuck
  • Joseph Katz
  • REBECCA HULL MEICHLE
  • Linda Brinkley
  • REBECCA LUCY HULL MEICHLE
  • Hisashi Shinozuka
  • Krisztina Nagy
  • Tusty Jiuan Hsieh
  • Richard B Marchase
  • John C Chatham
  • H Yildiz
  • Stéphanie Olivier-Van Stichelen
  • Anne Mathieu
  • Christiane Bierkamp
  • S Umair
  • G Réz
  • Tony Lefebvre
  • Jean Claude Michalski
  • Jing Ling Huang
  • M Takemoto
  • Gábor Réz
  • Zsolt Pálfia
  • Antonio López-Lozano
  • Angana Gupta Rajapakse
  • Szilveszter Tóth
  • I N Feklistova
  • Zhigang Huang
  • Songon An
  • Kei Wada
  • Vanessa Dehennaut
  • Jia Liu
  • Voraratt Champattanachai
  • X L Zheng
  • A J Almeida
  • K Nagy
  • Hideyuki Suzuki
  • Line Mariann Grønning
  • Hidehiko Kumagai
  • Celine Masclaux-Daubresse
  • Z Palfia
  • S Toth
  • Jiang Li
  • Lucien Pradayrol
  • Catherine Seva
  • Pascal Clerc
  • Marlene Dufresne
  • Daniel Fourmy
  • Francesco Andreozzi
  • Kirsten Bagh
  • Takashi Koyanagi
  • Yi Pang
  • Pierre Fustier
  • Julie R Ingelfinger
  • I George Fantus
  • Shao Ling Zhang
  • Pavel Hamet
  • Janos G Filep
  • John S D Chan
  • Isabel Franke
  • H Oztas
  • Sakeneh Zraika
  • Jerome M McDonald
  • Tsuneo Maegawa
  • Wiktor Bednarz
  • B J Tsuei
  • E Kalıpcı
  • A Koc

Detail Information

Publications113 found, 100 shown here

  1. ncbi Purification and characterization of NADH-dependent glutamate synthase from the silkworm fat body (Bombyx mori)
    C Hirayama
    National Institute of Sericultural and Entomological Science, Ibaraki, Japan
    Insect Biochem Mol Biol 28:473-82. 1998
    ..5, at which Km values for 2-oxoglutarate, glutamine and NADH were 17, 220 and 5.7 micro M, respectively. Azaserine, 6-diazo-5-oxonorleucine and p-chloromercuribenzoic acid were strong inhibitors of the activity...
  2. ncbi The hexosamine biosynthesis pathway regulates insulin secretion via protein glycosylation in mouse islets
    Sakeneh Zraika
    Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Parkville, VIC 3050, Australia
    Arch Biochem Biophys 405:275-9. 2002
    ..This finding may have implications for the development of type 2 diabetes, as chronic increase in flux through the hexosamine biosynthesis pathway may lead to the deterioration of beta-cell function via abnormal protein glycosylation...
  3. doi The hexosamine biosynthesis inhibitor azaserine prevents endothelial inflammation and dysfunction under hyperglycemic condition through antioxidant effects
    Angana Gupta Rajapakse
    Division of Physiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland
    Am J Physiol Heart Circ Physiol 296:H815-22. 2009
    ..We investigated whether the HBP inhibitor azaserine protects against hyperglycemia-induced endothelial damage dependently of HBP...
  4. ncbi Overexpression of cholecystokinin receptors in azaserine-induced neoplasms of the rat pancreas
    R H Bell
    Department of Pathology, Dartmouth Medical School, Hanover, New Hampshire 03756
    Cancer Res 52:3295-9. 1992
    ..b>Azaserine-induced pancreatic carcinoma (DSL6) had an increased high-affinity CCK receptor binding capacity of 122 +/- 23 (..
  5. ncbi Cellular autophagic capacity changes during azaserine-induced tumour progression in the rat pancreas. Up-regulation in all premalignant stages and down-regulation with loss of cycloheximide sensitivity of segregation along with malignant transformation
    Szilveszter Tóth
    Department of General Zoology, Lorand Eotvos University, Budapest, Pf 120, 1518, Hungary
    Cell Tissue Res 309:409-16. 2002
    ..of cytoplasmic quanta were highly increased in premalignant nodule cells 6 months after initiation by azaserine in the rat pancreas in vivo...
  6. ncbi Changes in cellular autophagic capacity during azaserine-initiated pancreatic carcinogenesis
    S Toth
    Department of General Zoology, Eotvos Lorand University, Budapest, Hungary
    Acta Biol Hung 52:393-401. 2001
    ..the possible changes of autophagic activity during the progression of rat pancreatic adenocarcinoma induced by azaserine. In the present study, autophagic capacity of the azaserine-induced premalignant and malignant cells were ..
  7. ncbi Overexpression of messenger RNA for cholecystokinin-A receptor and novel expression of messenger RNA for gastrin (cholecystokinin-B) receptor in azaserine-induced rat pancreatic carcinoma
    W Zhou
    Department of Surgery, University of Cincinnati College of Medicine, OH
    Carcinogenesis 14:2189-92. 1993
    ..the high-affinity cholecystokinin (CCK) receptor and the novel expression of the gastrin (CCK-B) receptor in the azaserine-induced rat pancreatic carcinoma DSL-6...
  8. pmc Inhibition of glycosaminoglycan synthesis and protein glycosylation with WAS-406 and azaserine result in reduced islet amyloid formation in vitro
    Rebecca L Hull
    Division of Metabolism, Endocrinology, and Nutrition, Veterans Affairs Puget Sound Health Care System and University of Washington, Seattle, Washington 98108, USA
    Am J Physiol Cell Physiol 293:C1586-93. 2007
    ..b>Azaserine, an inhibitor of the rate-limiting step of the hexosamine biosynthesis pathway, replicated the effects of WAS-..
  9. ncbi The cytotoxicity of DNA carboxymethylation and methylation by the model carboxymethylating agent azaserine in human cells
    M O'Driscoll
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, London, UK
    Carcinogenesis 20:1855-62. 1999
    ..The cytotoxicity of the model DNA carboxymethylating agent azaserine was investigated in human cells...
  10. ncbi Cellular autophagic capacity is highly increased in azaserine-induced premalignant atypical acinar nodule cells
    G Réz
    Department of General Zoology, Lorand Eotvos University, pf 330, H 1445 Budapest, Hungary grez2cerberus elte hu
    Carcinogenesis 20:1893-8. 1999
    ..into the possible changes in autophagic activity during progression of rat pancreatic adenocarcinoma induced by azaserine and promoted by a raw soya flour-containing pancreatotrophic diet...
  11. ncbi Transforming growth factor-alpha and epidermal growth factor expression in the exocrine pancreas of azaserine-treated rats: modulation by cholecystokinin or a low fat, high fiber (caloric restricted) diet
    C J Visser
    Department of Pathology, University Hospital Utrecht, The Netherlands
    Carcinogenesis 16:2075-82. 1995
    ..growth factor (EGF) was studied in normal pancreatic tissue and in (pre)neoplastic pancreatic lesions of azaserine-treated rats...
  12. ncbi Expression of c-myc, c-raf-1, and c-Ki-ras in azaserine-induced pancreatic carcinomas and growing pancreas in rats
    J A Silverman
    Department of Pathology, Dartmouth Medical School, Hanover, New Hampshire 03756
    Mol Carcinog 3:379-86. 1990
    ..We also investigated proto-oncogene expression associated with the progression of pancreatic cancers in azaserine-treated rats...
  13. pmc Dietary fish oil (MaxEPA) enhances pancreatic carcinogenesis in azaserine-treated rats
    M J Appel
    TNO Nutrition and Food Research Institute, Department of Pathology, Zeist, The Netherlands
    Br J Cancer 73:36-43. 1996
    In the present study the putative chemopreventive effect of dietary fish oil (MaxEPA) on azaserine-induced pancreatic carcinogenesis in rats was investigated...
  14. ncbi Role of the basic helix-loop-helix transcription factor p48 in the differentiation phenotype of exocrine pancreas cancer cells
    T Adell
    Unitat de Biologia Cellular i Molecular, Institut Municipal d Investigacio Medica, Universitat Pompeu Fabra, Barcelona, Spain
    Cell Growth Differ 11:137-47. 2000
    ..We have examined the role of p48 in the phenotype of azaserine-induced rat DSL6 tumors and cancers of the human exocrine pancreas...
  15. ncbi Transgenic expression of CCK2 receptors sensitizes murine pancreatic acinar cells to carcinogen-induced preneoplastic lesions formation
    Anne Mathieu
    INSERM U531, IFR31, Hospital Rangueil, Toulouse, France
    Int J Cancer 115:46-54. 2005
    ..initial steps of carcinogenesis in ElasCCK2 mice, adding a supplementary defect by using a chemical carcinogen, azaserine. Results of posttreatment sequential immunohistochemical examinations and quantifications demonstrate that mice ..
  16. ncbi Characterisation of the progression of azaserine-induced rat pancreatic adenocarcinoma by proliferative cell nuclear antigen, basement membrane laminin and trypsinogen immunohistochemistry
    Krisztina Nagy
    Department of General Zoology, Eotvos University, Pf 120, 1518 Budapest, Hungary
    Histochem Cell Biol 119:405-13. 2003
    The progression of azaserine-induced rat pancreatic adenocarcinoma (AC) was characterised using quantitative and semiquantitative immunohistochemistry for proliferating cell nuclear antigen (PCNA), basement membrane laminin (BML) and ..
  17. ncbi Angiogenesis is continuous with two peaks during azaserine-induced rat pancreatic adenocarcinoma progression: an electron microscopic morphometrical study
    Krisztina Nagy
    Department of General Zoology, Eotvos University, Budapest, H 1518, Hungary
    Oncol Rep 10:1999-2004. 2003
    ..In our study a further experimental system, the azaserine-induced rat pancreatic adenocarcinoma was applied for this purpose...
  18. ncbi Effects of a diet high in fish oil (MaxEPA) on the formation of micronucleated erythrocytes in blood and on the number of atypical acinar cell foci Induced in rat pancreas by azaserine
    Marko J Appel
    Department of General Toxicology, TNO Nutrition and Food Research, PO Box 360, 3700 AJ Zeist, The Netherlands
    Nutr Cancer 47:57-61. 2003
    The present study was performed to investigate the influence of fish oil on the genotoxic effects of azaserine, using the formation of micronucleated erythrocytes as a measure for the degree of initiating potency and the number and size ..
  19. pmc Expression of cholecystokinin-2/gastrin receptor in the murine pancreas modulates cell adhesion and cell differentiation in vivo
    Christiane Bierkamp
    Institut National de la Sante et de la Recherche Medicale, INSERM U531, Hospital Rangueil, 31059 Toulouse Cedex 9, France
    Am J Pathol 165:2135-45. 2004
    ..Finally, expression of the transgene in mice treated with the carcinogen azaserine resulted in enhanced size of preneoplastic lesions as well as an increased degree of acinar-ductal ..
  20. ncbi Effect of hypergastrinemia on pancreatic carcinogenesis
    Jerome M McDonald
    Department of Surgery, Veterans Affairs Puget Sound Health Care System and the University of Washington School of Medicine, Seattle, WA 98108, USA
    Am J Surg 183:441-4. 2002
    ..In view of an abundant literature suggesting that gastrin may promote growth of various gastrointestinal tissues and tumors, the effect of hypergastrinemia on the process of pancreatic carcinogenesis was evaluated...
  21. ncbi High glucose stimulates angiotensinogen gene expression and cell hypertrophy via activation of the hexosamine biosynthesis pathway in rat kidney proximal tubular cells
    Tusty Jiuan Hsieh
    Université de Montréal Centre hospitalier de l Université de Montréal Hôtel Dieu, Centre de Recherche Pavillon Masson, Montreal, Quebec, Canada H2W 1T8
    Endocrinology 144:4338-49. 2003
    ..b>Azaserine and 6-diazo-5-oxo-l-norleucine (inhibitors of glutamine: fructose-6-phosphate amino transferase enzyme) blocked ..
  22. ncbi Glucose induces increases in levels of the transcriptional repressor Id2 via the hexosamine pathway
    Line Mariann Grønning
    Department of Biochemistry and Molecular Biology, Guys Hospital, Kings College, London, United Kingdom
    Am J Physiol Endocrinol Metab 290:E599-606. 2006
    ..2) the effect of glucose is mimicked by low levels of glucosamine; 3) the effect of glucose is inhibited by azaserine, an inhibitor of glutamine:fructose-6-phosphate amidotransferase (GFAT); and 4) adenoviral mediated ..
  23. pmc Glutamine-induced protection of isolated rat heart from ischemia/reperfusion injury is mediated via the hexosamine biosynthesis pathway and increased protein O-GlcNAc levels
    Jia Liu
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Mol Cell Cardiol 42:177-85. 2007
    ..Pre-treatment with 80 muM azaserine, an inhibitor of GFAT, completely reversed the protection seen with glutamine and prevented the increase in ..
  24. ncbi Nutrient sensing by the early mouse embryo: hexosamine biosynthesis and glucose signaling during preimplantation development
    Marie Pantaleon
    School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland, 4072 Australia
    Biol Reprod 78:595-600. 2008
    ..These data suggest that early embryos rely on hexosamine biosynthesis as a glucose-sensing pathway to initiate metabolic differentiation...
  25. doi Exogenous ammonium inhibits petal pigmentation and expansion in Gerbera hybrida
    Zhigang Huang
    College of Life Sciences, South China Normal University, Guangdong Key Lab of Biotechnology for Plant Development, Guangzhou 510631, China
    Physiol Plant 133:254-65. 2008
    ....
  26. ncbi Poly-beta-hydroxybutyrate accumulation in Nostoc muscorum: effects of metabolic inhibitors
    Nirupama Mallick
    Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, India
    J Plant Physiol 164:312-7. 2007
    ..Supplementation of l-methionine-dl-sulfoximine (MSX) and azaserine was also found to increase PHB accumulation in N(2) -fixing and NH(4)(+) -grown N...
  27. ncbi Reactive oxygen species blockade and action of insulin on expression of angiotensinogen gene in proximal tubular cells
    Tusty Jiuan Hsieh
    Research Centre, Centre Hospitalier de l Universite de Montreal CHUM, Hotel Dieu, 3850 Saint Urbain Street, Montreal, Quebec H2W 1T8, Canada
    J Endocrinol 183:535-50. 2004
    ..enzyme (perindopril), protein kinase C (GF 109203X), or glutamine:fructose-6-phosphate amino-transferase (azaserine)...
  28. ncbi Glutamate synthesis in barley roots: the role of the plastidic glucose-6-phosphate dehydrogenase
    Sergio Esposito
    Dipartimento di Biologia Vegetale, Universita di Napoli Federico II, Via Foria 223, 80139 Naples, Italy
    Planta 216:639-47. 2003
    ..The whole process exhibited an apparent K(m Glc-6-P) of 0.45 mM and is abolished by azaserine, a specific inhibitor of GOGAT; ATP caused a decrease in the rate of Glu formation...
  29. ncbi Linkage between toxin production and purine biosynthesis in Clostridium difficile
    Tsuneo Maegawa
    Department of Bacteriology, School of Medicine, Kanazawa University, Japan
    J Med Microbiol 51:34-41. 2002
    ..Inhibition of the enzymic activity of C. difficile PurL by O-diazoacetyl-L-serine (azaserine) resulted in enhanced toxin B production even in biotin-sufficient conditions...
  30. ncbi Uptake and metabolism of adenosine mediate a meiosis-arresting action on mouse oocytes
    S M Downs
    Biology Department, Marquette University, Milwaukee, Wisconsin 53201 1881, USA
    Mol Reprod Dev 53:208-21. 1999
    ..Inhibition of de novo purine synthesis alone was not sufficient because azaserine failed to reverse meiotic arrest...
  31. ncbi Early phase karyotype analysis of chromosome segregation after formation of mouse-mouse hybridomas with chromosome painting probes
    L Wollweber
    Institut fur Molekulare Biotechnologie, Jena, Germany
    Chromosome Res 8:37-44. 2000
    ..653 mouse myeloma cells and lymphocytes from BALB/c mice by PEG fusion and by selection in hypoxanthine azaserine medium...
  32. ncbi Early indicators of exocrine pancreas carcinogenesis produced by non-genotoxic agents
    R A Woutersen
    Department of Biological Toxicology, TNO CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands
    Mutat Res 248:291-302. 1991
    ..from the putative, atypical preneoplastic, acinar cell foci induced in rat pancreas by the carcinogen azaserine. Unsaturated fat (corn oil) has been found to increase the incidence of atypical acinar cell nodules and ..
  33. ncbi Expression of genes involved in nitrogen assimilation and the C/N balance sensing in Prochlorococcus sp. strain SS120
    Antonio López-Lozano
    Departamento de Bioquimica y Biologia Molecular, Universidad de Cordoba, 14071 Cordoba, Spain
    Gene Expr 14:279-89. 2009
    ..Methionine sulfoximine and azaserine, inhibitors of glutamine synthetase and glutamate synthase, respectively, provoked a sharp increase in icd ..
  34. ncbi Hexosamine induction of oxidative stress, hypertrophy and laminin expression in renal mesangial cells: effect of the anti-oxidant alpha-lipoic acid
    Lalit P Singh
    Department of Anatomy Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell Biochem Funct 25:537-50. 2007
    ..7 +/- 0.2- and 1.4 +/- 0.04-fold, respectively, versus LG (p < 0.01 for both). Azaserine, an inhibitor of glutamine:fruc-6-PO(4) amidotransferase (GFAT) in the HBP, blocks the HG-induced expression of ..
  35. ncbi Tumour suppressor protein (p53), apoptosis inhibiting protein (Bcl-2) and proliferating cell nuclear antigen (PCNA) expressions in a rat pancreatic tumour model
    Kimmo Makinen
    Department of Vascular Surgery, Kuopio University Hospital, SF 70210 Kuopio, Finland
    Anticancer Res 27:23-6. 2007
    Previous studies have shown that, cultured rat pancreatic carcinoma cells, derived from azaserine-induced acinar tumours, yield tumours with a ductal phenotype.
  36. ncbi O-linked N-acetylglucosaminyltransferase inhibition prevents G2/M transition in Xenopus laevis oocytes
    Vanessa Dehennaut
    Laboratoire de Régulation des Signaux de Division, EA 4020, Universite des Sciences et Technologies de Lille, SN3, IFR147, 59655 Villeneuve d Ascq, France
    J Biol Chem 282:12527-36. 2007
    ..Glutamine:fructose-6-phosphate amidotransferase inhibitors, azaserine and 6-diazo-5-oxonorleucine, failed to prevent GVBD...
  37. ncbi Hypoxanthine regulation of oocyte maturation in the mouse: insights using hypoxanthine phosphoribosyltransferase-deficient animals
    S M Downs
    Biology Department, Marquette University, Milwaukee, Wisconsin 53233, USA
    Biol Reprod 57:54-62. 1997
    ..with FSH, GVB was blocked to the same extent in HPRT- oocytes with the purine de novo synthesis inhibitor, azaserine, but this drug was less effective in HX-treated HPRT+ oocytes...
  38. ncbi Determination of NADH-dependent glutamate synthase (GOGAT) in Spodoptera frugiperda (Sf9) insect cells by a selective 1H/15N NMR in vitro assay
    M Doverskog
    Department of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    J Biotechnol 79:87-97. 2000
    ..The enzyme activity was exclusively dependent on NADH as coenzyme and was completely inhibited by 1 mM azaserine. From the results obtained, we conclude that Sf9 cells possess NADH-GOGAT activity...
  39. ncbi Phenotype-specific inhibition of the vascular smooth muscle cell cycle by high glucose treatment
    X L Zheng
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary Health Sciences Centre, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada
    Diabetologia 50:881-90. 2007
    ..Considering the established SMC heterogeneity, we hypothesised that glucose treatment may have distinct effects on proliferation of the various phenotypic SMCs...
  40. ncbi A novel profile of expressed sequence tags for zinc finger encoding genes from the poorly differentiated exocrine pancreatic cell line AR4IP
    B Gebelein
    Department of Molecular Neuroscience, Mayo Clinic, Rochester, MN 55905, USA
    Cancer Lett 105:225-31. 1996
    ..the primary structure and expression pattern of 18 different zinc finger-encoding cDNAs (DZF-1-18) from the azaserine-derived tumoral cell line AR4IP which displays a poorly differentiated phenotype...
  41. pmc The primary function of a redundant Sp1 binding site in the mouse aprt gene promoter is to block epigenetic gene inactivation
    P Mummaneni
    Department of Pathology, University of Kentucky, Lexington, KY 40536, USA
    Nucleic Acids Res 26:5163-9. 1998
    ..Therefore, the Sp1 binding sites comprising the mouse aprt promoter have evolved two distinct functions, one to promote transcription and the other to block epigenetic inactivation...
  42. ncbi Biallelic methylation and silencing of mouse Aprt in normal kidney cells
    J A Rose
    Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, Portland 97201, USA
    Cancer Res 60:3404-8. 2000
    ..A potential role for allelic homology in these events is discussed...
  43. doi Crystal structures of Escherichia coli gamma-glutamyltranspeptidase in complex with azaserine and acivicin: novel mechanistic implication for inhibition by glutamine antagonists
    Kei Wada
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Mol Biol 380:361-72. 2008
    ..b>Azaserine and acivicin are classical and irreversible inhibitors of GGT, but their binding sites and the inhibition ..
  44. ncbi Towards a molecular genetic system for the pathogenic fungus Paracoccidioides brasiliensis
    A J Almeida
    Life and Health Sciences Research Institute ICVS, School of Health Sciences, University of Minho, Braga, Portugal
    Fungal Genet Biol 44:1387-98. 2007
    ..Moreover, we present evidence for the occurrence of random single gene copy integration per haploid nuclei and the generation of homokaryon progeny, relevant for the future use in targeted mutagenesis and linking mutations to phenotypes...
  45. ncbi Flux through the hexosamine pathway is a determinant of nuclear factor kappaB- dependent promoter activation
    Leighton R James
    Department of Medicine, University of Toronto, Toronto, Ontario, Canada
    Diabetes 51:1146-56. 2002
    ..8-fold, and these increases were prevented by 40 micromol/l O-diazoacetyl-L-serine (azaserine) or 6-diazo-5-oxonorleucine...
  46. ncbi Safety of umbilical cord blood-derived mesenchymal stem cells (MSCs) following 5-azaserine induction and inhibition of human cardiac myocyte apoptosis by MSCs
    Jing Ling Huang
    School of Life Science, Lanzhou University, Lanzhou, Gansu, China
    Saudi Med J 30:1144-9. 2009
    ..To further study the safety and effect of the umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) on apoptosis of human cardiac myocyte (HCM)...
  47. pmc Pancreatobiliary diversion enhances experimental pancreatic carcinogenesis
    I D Stewart
    Department of Surgery, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK
    Br J Cancer 63:63-6. 1991
    ..Postoperatively, each group received azaserine (20 mg kg-1 wk-1 i.p.) for 6 weeks...
  48. ncbi Enhanced expression of osteopontin by high glucose. Involvement of osteopontin in diabetic macroangiopathy
    M Takemoto
    Second Department of Internal Medicine, School of Medicine, Chiba University, Japan
    Ann N Y Acad Sci 902:357-63. 2000
    ..Glucosamine also increased the promoter activity of osteopontin. Azaserine, an inhibitor of glutamine:fructose-6-phosphate amidotransferase, the key enzyme of the hexosamine pathway, ..
  49. pmc The growth pattern and microvasculature of pancreatic tumours induced with cultured carcinoma cells
    K Makinen
    Department of Surgery, Kuopio University Hospital, Finland
    Br J Cancer 82:900-4. 2000
    ..after intrapancreatic injection of cultured pancreatic carcinoma cells (DSL-6A/C1), originally derived from an azaserine-induced tumour, as well as the features of tumour microcirculation using the microangiography technique...
  50. ncbi Enhanced expression of osteopontin by high glucose in cultured rat aortic smooth muscle cells
    M Takemoto
    School of Medicine, Chiba University, 1 8 1 Inohana, Chiba, Chuou ku, 260 0856, Japan
    Biochem Biophys Res Commun 258:722-6. 1999
    ..Glucosamine also increased the promoter activity of osteopontin, and azaserine, an inhibitor of glutamine:fructose-6-phosphate amidotransferase (the key enzyme of the hexosamine pathway), ..
  51. ncbi Adenosine blocks hormone-induced meiotic maturation by suppressing purine de novo synthesis
    S M Downs
    Biology Department, Marquette University, Milwaukee, Wisconsin 53233, USA
    Mol Reprod Dev 56:172-9. 2000
    ..mice, hCG-induced maturation was blocked by Ado as effectively as by the purine de novo synthesis inhibitor, azaserine. It is concluded that Ado has an inhibitory action on hormone-induced maturation that is due, at least in part, ..
  52. ncbi [Isolation of Pseudomonas aurantiaca strains capable of overproduction of phenazine antibiotics]
    I N Feklistova
    Mikrobiologiia 77:207-12. 2008
    ..NH)-induced mutagenesis with subsequent selection for resistance to toxic amino acid analogues (azaserine, m-fluoro-DL-phenylalanine, and 6-diazo-5-oxo-L-norleucine) was applied to Pseudomonas aurantiaca B-162...
  53. pmc YfiK from Escherichia coli promotes export of O-acetylserine and cysteine
    Isabel Franke
    Department Biologie I, Mikrobiologie, Universität München Munich, Germany
    J Bacteriol 185:1161-6. 2003
    ..Overexpression of either ydeD or yfiK leads to alleviation of this inhibition paralled by increased resistance to azaserine, which is an analog of O-acetylserine.
  54. ncbi Human GMP synthetase
    T Page
    Int J Biochem 16:117-20. 1984
    ..The enzyme was specific for ATP as the energy source. Unlike the calf thymus enzyme, the human enzyme has no requirement for a reduced sulfhydryl compound. Human GMP synthetase is inhibited by ATP, dATP, azaserine, and hydroxylamine.
  55. pmc Relationship between NH(4) Assimilation Rate and in Vivo Phosphoenolpyruvate Carboxylase Activity : Regulation of Anaplerotic Carbon Flow in the Green Alga Selenastrum minutum
    G C Vanlerberghe
    Department of Biology, Queen s University, Kingston, Ontario, Canada, K7L 3N6
    Plant Physiol 94:284-90. 1990
    ..Treatment of N-limited cells with azaserine caused an increase in the Gln/Glu ratio resulting in increased PEPC activity in the absence of N assimilation...
  56. doi Inhibitory effects of acetylsalicylic acid on exocrine pancreatic carcinogenesis
    H Yildiz
    Mustafa Kemal University, Faculty of Science, Department of Biology, Antakya
    Biotech Histochem 88:132-7. 2013
    ..We investigated possible inhibitory effects of acetylsalicylic acid in an azaserine-treated rat model. AACF were produced in rats by injection with azaserine according to previous studies...
  57. ncbi Hexosamine pathway is responsible for inhibition by diabetes of phenylephrine-induced inotropy
    Yi Pang
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Diabetes 53:1074-81. 2004
    ..The attenuation of CCE was also seen with glucosamine, and the inhibition by hyperglycemia was prevented by azaserine, thereby implicating hexosamine biosynthesis as the responsible metabolic pathway...
  58. doi Ischemic preconditioning increases myocardial O-GlcNAc glycosylation
    Rebekka Vibjerg Jensen
    Department of Cardiology, Aarhus University Hospital, Skejby, Aarhus N, Denmark
    Scand Cardiovasc J 47:168-74. 2013
    ..b>Azaserine and alloxan are unspecific blockers of the HBP and have been used to block the cardioprotective effects of O-..
  59. doi Identification and characterization of genes related to the production of organic acids in yeast
    Satoshi Yoshida
    Central Laboratories for Frontier Technology, Kirin Holdings Co, Ltd, 1 13 5 Fukuura, Kanazawa Ku, Yokohama shi, Kanagawa 236 0004, Japan
    J Biosci Bioeng 113:556-61. 2012
    ..cerevisiae disruptants involved in organic acid production showed that azaserine, citrate, ethionine, and sulfite are useful compounds by which mutants with altered organic acid production ..
  60. doi The hexosamine biosynthetic pathway and O-GlcNAcylation drive the expression of β-catenin and cell proliferation
    Stéphanie Olivier-Van Stichelen
    CNRS UMR 8576, Unit of Structural and Functional Glycobiology, University of Lille 1, Villeneuve d Ascq, France
    Am J Physiol Endocrinol Metab 302:E417-24. 2012
    ..signaling heavily expressed β-catenin when cultured in high glucose; this was reversed by the HBP inhibitor azaserine. HBP inhibition also decreased the expression of β-catenin in HT29 and, to a lesser extent, HCT116 cells...
  61. ncbi Inhibitory effects of estrogen and castration on the early stage of pancreatic carcinogenesis in Fischer rats treated with azaserine
    C Sumi
    Department of Pathology, Dartmouth Medical School, Hanover, New Hampshire 03756
    Cancer Res 49:2332-6. 1989
    Effects of sex steroids on pancreatic carcinogenesis during the early stage were studied in azaserine-treated rats of both sexes. Fischer rats were given weekly i.p...
  62. pmc Potentiation of azaserine by cholestyramine in the rat
    R G Morgan
    Department of Physiology, University of Western Australia
    Int J Exp Pathol 71:485-91. 1990
    ..an increased incidence of spontaneous pancreatic neoplasms and marked potentiation of the pancreatic carcinogen azaserine. Since previous studies showed that cholestyramine caused pancreatic growth in this species we have now studied ..
  63. ncbi Ammonia assimilation by Aspergillus nidulans: [15N]ammonia study
    M B Kusnan
    Fachbereich Biologie, Universität Kaiserlautern, FRG
    J Gen Microbiol 135:729-38. 1989
    ..In the presence of azaserine, an inhibitor of glutamate synthase, glutamate was initially more highly labelled than any other amino acid, ..
  64. ncbi Thrazarine, a new antitumor antibiotic. I. Taxonomy, fermentation, isolation and biological properties
    T Kameyama
    Institute of Microbial Chemistry, Tokyo, Japan
    J Antibiot (Tokyo) 41:1561-7. 1988
    ..It showed neither antimicrobial activity nor the inhibition of transamidation reactions in contrast to azaserine. Toxicities of thrazarine were much weaker than those of azaserine.
  65. pmc Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco
    Celine Masclaux-Daubresse
    Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
    Plant Physiol 140:444-56. 2006
    ..Taken together, the data provide evidence that the GS-GOGAT pathway and GDH play distinct roles in the source-sink nitrogen cycle of tobacco leaves...
  66. ncbi Influence of cholecystokinin antagonist on the effects of cholecystokinin and bombesin on azaserine-induced lesions in rat pancreas
    B R Douglas
    Department of Gastroenterology and Hepatology, University Hospital, Leiden, The Netherlands
    Gastroenterology 96:462-9. 1989
    Both cholecystokinin and bombesin have been shown to promote pancreatic carcinogenesis in the azaserine-rat model...
  67. ncbi Nitrogen-15 NMR studies of nitrogen metabolism in Picea glauca buds
    Kirsten Bagh
    Division of Biochemistry, Department of Biological Sciences, University of Calgary, Calgary, Alta, Canada T2N 1N4
    Plant Physiol Biochem 42:803-9. 2004
    ..were carried out involving the use of inhibitors of the nitrogen pathways such as methionine sulfoximine (MSO), azaserine (AZA) and aminooxyacetate (AOA)...
  68. ncbi Modulation of dietary fat-promoted pancreatic carcinogenesis in rats and hamsters by chronic coffee ingestion
    R A Woutersen
    Department of Biological Toxicology, TNO CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands
    Carcinogenesis 10:311-6. 1989
    ..Rats were given a single i.p. injection of 30 mg azaserine per kg body weight at 19 days of age. Hamsters were injected s.c...
  69. ncbi [Establishment of an IR-HIRc cell model for screening GFAT inhibitor]
    Jiang Li
    Institute of Materia Medica, Chinese Academy of Medical Sciences, China
    Yao Xue Xue Bao 40:418-22. 2005
    ..To set up an IR-HIRc cell model for screening the inhibitor of GFAT (glutamine: fructose-6-phosphate amidotransferase) , the key enzyme in the hexosamine biosynthesis pathway (HBP)...
  70. ncbi Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein-associated O-GlcNAc
    Voraratt Champattanachai
    University of Alabama at Birmingham, 1530 3rd Avenue South, MCLM 684, Birmingham, AL 35294 0005, USA
    Am J Physiol Cell Physiol 292:C178-87. 2007
    ..The improved survival with hyperglycemia was attenuated by azaserine, which inhibits glucose metabolism via the hexosamine biosynthesis pathway...
  71. doi Glutamate synthase, but not GABA shunt enzymes, contributes to nitrogen metabolism of the sheep abomasal nematode parasites Haemonchus contortus and Teladorsagia circumcincta
    S Umair
    Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand
    Exp Parasitol 127:9-13. 2011
    ..GOGAT activity was inhibited by 80% by azaserine. Activity (nmol min(-1) mg(-1) protein) was 33-59 in adult H. contortus, 51-91 in adult T...
  72. ncbi Molecular and biochemical elucidation of a cellular phenotype characterized by adenine analogue resistance in the presence of high levels of adenine phosphoribosyltransferase activity
    N H Khattar
    Department of Microbiology and Immunology, University of Kentucky College of Medicine, Lexington 40536
    Biochem Genet 30:635-48. 1992
    ..H29D1, also exhibited near-wild-type levels of adenine accumulation and the ability to grow in medium containing azaserine and adenine. Growth in this medium requires high levels of intracellular APRT activity...
  73. ncbi Azaserine-induced pancreatic foci: detection, growth, labelling index and response to raw soya flour
    J M Daly
    Department of Physiology, University of Western Australia, Nedlands
    Carcinogenesis 13:1519-23. 1992
    Atypical acinar cell foci were induced in the pancreases of rats by injection of azaserine. An incubation period of 6 weeks was sufficient for the detection of all glutathione S-transferase mu positive foci...
  74. ncbi Novel expression of gastrin (cholecystokinin-B) receptors in azaserine-induced rat pancreatic carcinoma: receptor determination and characterization
    W Zhou
    Department of Surgery, University of Cincinnati College of Medicine, Ohio 45267
    Cancer Res 52:6905-11. 1992
    ..In the present study, we investigated gastrin (CCK-B) receptor expression in the azaserine-induced rat pancreatic carcinoma DSL-6, comparing it to normal rat pancreas, and we also characterized CCK ..
  75. doi Reversible compartmentalization of de novo purine biosynthetic complexes in living cells
    Songon An
    Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA
    Science 320:103-6. 2008
    ..Collectively, the data provide strong evidence for the formation of a multi-enzyme complex, the "purinosome," to carry out de novo purine biosynthesis in cells...
  76. pmc Enhancing effect of partial gastrectomy on pancreatic carcinogenesis
    P Watanapa
    Department of Surgery, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK
    Br J Cancer 65:383-7. 1992
    ..p. injections of either saline or azaserine (30 mg kg-1 wk-1 for 3 weeks)...
  77. ncbi Insulin regulation of pyruvate kinase activity in isolated adipocytes. Crucial role of glucose and the hexosamine biosynthesis pathway in the expression of insulin action
    R R Traxinger
    Department of Biochemistry, University of Tennessee, Memphis 38163
    J Biol Chem 267:9718-23. 1992
    ..Considered together, these findings provide new insights into the cascade of metabolic events triggered by insulin and implicated a novel metabolic pathway in the pretranslational control of enzyme function...
  78. ncbi Antifolates induce inhibition of amido phosphoribosyltransferase in leukemia cells
    M E Sant
    Department of Biochemistry, University of Sydney, New South Wales, Australia
    J Biol Chem 267:11038-45. 1992
    ..derivatives of N-formylglycinamide ribotide (FGAR) which accumulate when the pathway is blocked completely by azaserine. This azaserine-induced accumulation of FGAR polyphosphates is completely abolished by MTX, indicating that ..
  79. ncbi Regulation of purine synthesis de novo in human fibroblasts by purine nucleotides and phosphoribosylpyrophosphate
    M A Becker
    Department of Medicine, University of Chicago Pritzker School of Medicine, Illinois 60637
    J Biol Chem 262:14531-7. 1987
    ..Incubation of cells for 90 min with 0.1 mM azaserine, a glutamine antagonist which specifically blocked the pathway at the level of conversion of formylglycinamide ..
  80. ncbi Affinity of antineoplastic amino acid drugs for the large neutral amino acid transporter of the blood-brain barrier
    Y Takada
    Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892
    Cancer Chemother Pharmacol 29:89-94. 1991
    ..Of the six drugs tested, five, including melphalan, azaserine, acivicin, 6-diazo-5-oxo-L-norleucine, and buthionine sulfoximine, exhibited only low affinity for the carrier, ..
  81. ncbi Evaluation of promotion of pancreatic carcinogenesis in rats by benzyl acetate
    D S Longnecker
    Department of Pathology, Dartmouth Medical School, Hanover, NH 03756
    Food Chem Toxicol 28:665-8. 1990
    ..Significant stimulation of growth of azaserine-induced foci was observed in a 6-month study, and a low but significant incidence of carcinoma in situ was ..
  82. ncbi Antifolates induce primary inhibition of the de novo purine pathway prior to 5-aminoimidazole-4-carboxamide ribotide transformylase in leukemia cells
    S D Lyons
    Department of Biochemistry, University of Sydney, NSW, Australia
    Biochem Int 24:187-97. 1991
    ..By contrast, complete inhibition of reaction 4 by 25 microM azaserine results in accumulation of N-formyl-glycinamide ribotide (FGAR) polyphosphates to a combined cellular ..
  83. ncbi The LIV-I/LS system as a determinant of azaserine sensitivity of Escherichia coli K-12
    Takashi Koyanagi
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo ku, Kyoto 606 8502, Japan
    FEMS Microbiol Lett 237:73-7. 2004
    The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine)...
  84. ncbi Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin insulin biosynthetic pathway in RIN pancreatic beta-cells
    Francesco Andreozzi
    Dipartimento di Medicina Sperimentale e Clinica, Università di Catanzaro Magna Graecia, Via Tommaso Campanella, 115, 88100 Catanzaro, Italy
    Endocrinology 145:2845-57. 2004
    ..b>Azaserine reverted the stimulatory effects of high glucose on JNK and ERK1/2 activity and IRS-1 phosphorylation at Ser(307)..
  85. ncbi Purification of, generation of monoclonal antibodies to, and mapping of phosphoribosyl N-formylglycinamide amidotransferase
    T S Barnes
    Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado 80206
    Biochemistry 33:1850-60. 1994
    ..The CHO Azr cell line was made resistant to azaserine, a potent inhibitor of FGAR amidotransferase, and displays a 5-10-fold increase in FGAR amidotransferase ..
  86. ncbi Glutamine metabolism in endothelial cells: ornithine synthesis from glutamine via pyrroline-5-carboxylate synthase
    G Wu
    Department of Animal Science and Faculty of Nutrition, Texas A and M University, College Station 77843, USA
    Comp Biochem Physiol A Mol Integr Physiol 126:115-23. 2000
    ..This new knowledge of glutamine metabolism may pave a new path for understanding the physiological role of glutamine in vascular function...
  87. ncbi Marginal zinc status does not exacerbate pancreatic carcinogenesis associated with dietary soybean trypsin inhibitor concentrate in rats
    K C Ellwood
    Division of Science and Applied Technology, Office of Special Nutritionals, Food and Drug Administration, Laurel, MD 20708
    J Nutr 124:894-900. 1994
    ..Male Sprague-Dawley rats (14 d of age) were given a single injection of either saline or azaserine and were weaned (21 d) to diets with either adequate (30 micrograms/g) or low (9 micrograms/g) zinc, with or ..
  88. ncbi Caloric restriction and intervention in pancreatic carcinogenesis in the rat
    B D Roebuck
    Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire 03755 3835
    Cancer Res 53:46-52. 1993
    ..Male Lewis rats were given injections of azaserine at 14 days of age and weaned to the postinitiation test protocols at 21 days of age...
  89. ncbi Gastrin receptor expression during azaserine-induced rat pancreatic carcinogenesis
    B J Tsuei
    Department of Surgery, University of Cincinnati, Ohio 45221, USA
    J Surg Res 63:105-9. 1996
    ..Therefore, in the present study, we examined rat pancreatic tissue at various stages in azaserine-induced pancreatic carcinogenesis for gastrin binding and for the presence of gastrin receptor mRNA to determine ..
  90. ncbi Inhibition by verapamil of cholecystokinin-enhancement of pancreatic carcinogenesis induced by azaserine in Wistar rats
    A Nakaizumi
    Department of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan
    Cancer Lett 105:23-7. 1996
    ..calcium channel blocker, verapamil, on cholecystokinin (CCK)-enhancement of pancreatic carcinogenesis induced by azaserine was investigated in Wistar rats...
  91. ncbi Regulation of cholecystokinin-mediated amylase secretion by leptin in rat pancreatic acinar tumor cell line AR42J
    D M Harris
    Center for Human Nutrition, UCLA School of Medicine, Los Angeles, California, USA
    Pancreas 19:224-30. 1999
    ..AR42J, a cell line derived from azaserine-treated rat pancreas, is a common model for pancreatic acinar cell secretion...
  92. ncbi Effects of dietary beta-carotene and selenium on initiation and promotion of pancreatic carcinogenesis in azaserine-treated rats
    M J Appel
    Department of General Toxicology, TNO Nutrition and Food Research Institute, The Netherlands
    Carcinogenesis 17:1411-6. 1996
    ..or HMix) on the initiation/early promotion phase or on the late promotion phase of pancreatic carcinogenesis in azaserine-treated rats, were investigated using cell proliferation and volumetric data of atypical acinar cell foci (AACF) ..
  93. ncbi Autoradiographic localization of cholecystokinin (CCK) receptor expression during the development of azaserine-induced rat pancreatic carcinoma
    B J Tsuei
    Department of Veterans Affairs Medical Center, Seattle, WA 98108, USA
    Pancreas 13:401-6. 1996
    The peptide hormone cholecystokinin (CCK) has been shown to stimulate the growth of azaserine-induced preneoplastic nodules in the rat pancreas...
  94. ncbi gamma-Glutamyl hydrolase from human sarcoma HT-1080 cells: characterization and inhibition by glutamine antagonists
    M C Waltham
    Program of Molecular Pharmacology and Therapeutics, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Pharmacol 51:825-32. 1997
    ..e., 6-diazo-5-oxo-norleucine (DON), acivicin, and azaserine] were identified as human GGH inhibitors, with DON being the most potent and displaying time-dependent ..
  95. ncbi 15N-NMR study of ammonium assimilation in Agaricus bisporus
    J J Baars
    Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands
    Biochim Biophys Acta 1310:74-80. 1996
    ..was determined by inhibiting glutamine synthetase with phosphinothricin and glutamate synthase with azaserine. Our results clearly indicate that, under the conditions used, ammonium assimilation is mainly catalysed by the ..
  96. ncbi Azaserine-induced rat pancreas tumor model with transplantable cultured cells
    K Makinen
    Department of Surgery, Kuopio University Hospital, Finland
    Pancreas 16:160-4. 1998
    ..According to our results, subcutaneous as well as intrapancreatic tumor induction with cultured cells offers a model for pancreatic cancer studies...
  97. pmc High glucose-induced transforming growth factor beta1 production is mediated by the hexosamine pathway in porcine glomerular mesangial cells
    V Kolm-Litty
    Institute for Diabetes Research, D 80804 München, Germany
    J Clin Invest 101:160-9. 1998
    ..Inhibition of GFAT by the substrate analogue azaserine or by inhibition of GFAT protein synthesis with antisense oligonucleotide prevented the high glucose-induced ..
  98. ncbi Sterol regulatory element-binding protein-1 is regulated by glucose at the transcriptional level
    A H Hasty
    Department of Metabolic Diseasese, University of Tokyo, Tokyo 113 8655, Japan
    J Biol Chem 275:31069-77. 2000
    ..These data provide strong evidence for the induction of SREBP-1c mRNA by glucose leading to increased mature protein in the nucleus, thus providing a potential mechanism for the up-regulation of lipogenic genes by glucose in vivo...
  99. pmc Glutamine metabolism to glucosamine is necessary for glutamine inhibition of endothelial nitric oxide synthesis
    G Wu
    Department of Animal Science and Faculty of Nutrition, Texas A and M University, 2471 TAMUS, College Station, TX 77843 2471, USA
    Biochem J 353:245-52. 2001
    ..2 or 2 mM L-glutamine with or without 20 microM 6-diazo-5-oxo-L-norleucine (DON) or with 100 microM azaserine. Both DON and azaserine are inhibitors of L-glutamine:D-fructose-6-phosphate transaminase (isomerizing) (EC 2.6...
  100. ncbi Inhibition of dietary fat promoted development of (pre)neoplastic lesions in exocrine pancreas of rats and hamsters by supplemental selenium and beta-carotene
    R A Woutersen
    Department of Biological Toxicology, TNO CIVO Toxicology and Nutrition Institute, Zeist, Netherlands
    Cancer Lett 42:79-85. 1988
    ..of putative preneoplastic foci in exocrine pancreas were investigated in rats and hamsters, treated with azaserine and N-nitrosobis(2-oxopropyl)amine (BOP), respectively...
  101. pmc Cross resistance pattern towards anticancer drugs of a human carcinoma multidrug-resistant cell line
    R S Gupta
    Department of Biochemistry, McMaster University, Hamilton, Canada
    Br J Cancer 58:441-7. 1988
    ..no significant cross-resistance to a large number of other drugs which included asaley, AT-125, 5-azacytidine, azaserine, cyclocytidine, cis-platin, cytosine arabinoside, chlorambucil, chlorpromazine, alpha-difluoromethyl ornithine, ..

Research Grants28

  1. Elucidating modulators of hepatic metabolism by quantitative flux analysis
    Gregory Stephanopoulos; Fiscal Year: 2010
    ..to the study of specific hormones (leptin), drugs (Metformin) and HBP flux modulators (hexosamine, aloxan, azaserine) of interest to Type-2-Diabetes...
  2. Elucidating modulators of hepatic metabolism by quantitative flux analysis
    Gregory Stephanopoulos; Fiscal Year: 2007
    ..to the study of specific hormones (leptin), drugs (Metformin) and HBP flux modulators (hexosamine, aloxan, azaserine) of interest to Type-2-Diabetes...
  3. Arginine and Ovine Model for Fetal Growth Restriction
    Guoyao Wu; Fiscal Year: 2007
    ..This will ultimately promote favorable pregnancy outcomes while saving billions of healthcare dollars in the U.S. and worldwide. ..
  4. Bridges to the Doctorate in Reproductive Biology
    Fuller Bazer; Fiscal Year: 2007
    ....
  5. OVINE INTERFERON-TAU REGULATES UTERINE HORMONE RECEPTOR
    Fuller Bazer; Fiscal Year: 2005
    ....
  6. Role of perlecan in islet amyloid formation
    REBECCA HULL MEICHLE; Fiscal Year: 2007
    ..Successful funding of this application will provide me with salary and protected research time in order to achieve my career goal of becoming an independent investigator in the field of type 2 diabetes. ..
  7. Scutellaria baicalensis and Colon Cancer Chemoprevention
    Diane Harris; Fiscal Year: 2005
    ..abstract_text> ..
  8. Islet Endothelial Dysfunction in Diabetes
    REBECCA LUCY HULL MEICHLE; Fiscal Year: 2010
    ..The focus of this proposal is the novel hypothesis that endothelial dysfunction occurs in the pancreatic islet in diabetes and contributes to decreased insulin release. ..