Genomes and Genes
acidic amino acids
Summary: Amino acids with side chains that are negatively charged at physiological pH.
Publications184 found, 100 shown here
- CDC7 kinase phosphorylates serine residues adjacent to acidic amino acids in the minichromosome maintenance 2 proteinWon Ho Cho
Department of Biology Education, Seoul National University, Seoul 151 742, Korea
Proc Natl Acad Sci U S A 103:11521-6. 2006..This finding may explain why the sequential action of cyclin-dependent and Cdc7 kinases is essential for the initiation of DNA replication...
- Utilization of acidic amino acids and their amides by pseudomonads: role of periplasmic glutaminase-asparaginaseAvinash Sonawane
Institute of Physiological Chemistry, Philipps University, Marburg (Lahn, Germany
Arch Microbiol 179:151-9. 2003The acidic amino acids (Asp, Glu) and their amides (Asn, Gln) support rapid growth of a variety of Pseudomonas strains when provided as the sole source of carbon and nitrogen. All key enzymes of glutamate metabolism were detected in P...
- Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2' of substratesMattias K Andersson
Department of Cell and Molecular Biology, Uppsala University, The Biomedical Center, Uppsala, Sweden
FEBS J 277:2255-67. 2010..of eight such chymases have been determined, and four of these were shown to have a strong preference for acidic amino acids at position P2'. These enzymes have basic amino acids in positions 143 and 192 (Arg and Lys, respectively)...
- Acidic amino acids impart enhanced Ca2+ permeability and flux in two members of the ATP-gated P2X receptor familyDamien S K Samways
Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, MO 63104, USA
J Gen Physiol 129:245-56. 2007..2001). Here, we test the hypothesis that the formal charge of acidic amino acids underlies the higher fractional Ca2+ currents (Pf%) of the rat and human P2X1 and P2X4 subtypes...
- Structure of calmodulin complexed with an olfactory CNG channel fragment and role of the central linker: residual dipolar couplings to evaluate calmodulin binding modes outside the kinase familyGian Marco Contessa
Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy
J Biomol NMR 31:185-99. 2005..Excluded regions comprise calcium binding loops and loops connecting the EF-hand motifs...
- Covalent cross-linking of proteins without chemical reagentsBrigitte L Simons
Centre for Biologics Research, Biologics and Genetic Therapies Directorate, Health Canada, Ottawa, Canada K1A 0L2
Protein Sci 11:1558-64. 2002....
- A novel theory on the origin of the genetic code: a GNC-SNS hypothesisKenji Ikehara
Department of Chemistry, Faculty of Science, Nara Women s University, Kita Uoya Nishi machi, Nara 630 8506, Japan
J Mol Evol 54:530-8. 2002..Thus, we concluded that the universal genetic code originated not from a three-amino acid system but from a four-amino acid system, the GNC code encoding [GADV]-proteins, as the most primitive genetic code...
- DNA binding provides a molecular strap activating the adenovirus proteinaseSayan Gupta
Center for Synchrotron Biosciences, Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Mol Cell Proteomics 3:950-9. 2004..In addition, both pVIc and DNA may independently alter the active site conformation as well as drive it cooperatively to fully activate AVP...
- An acidic peptide sequence of nucleolin-related protein can mediate the attachment of calcium oxalate to renal tubule cellsElena A Sorokina
Nephrology Division, Medical College of Wisconsin, Milwaukee, WI, USA
J Am Soc Nephrol 15:2057-65. 2004..These results support the notion that surface-associated NRP could mediate attachment of CaOx to the renal tubule epithelium, thereby causing retention of crystals that might eventually become kidney stones...
- Increasing the potency of a cytotoxin with an arginine graftStephen M Fuchs
Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
Protein Eng Des Sel 20:505-9. 2007..These findings correlate the potency of a ribonuclease with its deliverance of ribonucleolytic activity to the cytosol, and indicate a rational means to enhance the efficacy of ribonucleases and other cytotoxic proteins...
- Distance dependence of interactions between charged centres in proteins with common structural featuresRicardo O Louro
Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apt 127, 2780 156 Oeiras, Portugal
FEBS Lett 576:77-80. 2004..However, there is considerable scatter in the data despite the structural similarities among the proteins, which is discussed in the framework of using such simple models in predicting properties of novel proteins...
- Solution structure of coactosin reveals structural homology to ADF/cofilin family proteinsMaarit Hellman
Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Finland
FEBS Lett 576:91-6. 2004..Our analysis also identifies key structural differences between these proteins that may account for the differences in biochemical activities and cellular roles of these proteins...
- Structural basis for a non-phosphorus-containing cyclic peptide binding to Grb2-SH2 domain with high affinityPeng Li
Laboratory of Medicinal Chemistry, National Cancer Institute, National Institutes of Health, 376 Boyles Street, Frederick, MD 21702-1201, USA
Biochem Biophys Res Commun 307:1038-44. 2003..Whole cell assays indicate that peptide 20 can penetrate the cell membranes and inhibit the association of Grb2 with p185(erbB2) in erbB2-overexpressing MDA-MA-453 cancer cells at low micromolar concentrations...
- Functional analysis of acidic amino acids in the cytosolic tail of the Na+/H+ exchangerXiuju Li
Department of Biochemistry, 347 Medical Science Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochemistry 43:16477-86. 2004..Our results demonstrate that the acidic sequence is critical in maintaining proper conformation of the cytosolic domain, calmodulin binding, and in maintenance of Na(+)/H(+) exchanger activity...
- Charged amino acids of the N-terminal domain are involved in coupling binding and gating in alpha7 nicotinic receptorsFrancisco Sala
Instituto de Neurociencias, Universidad Miguel Hernandez Consejo Superior de Investigaciones Cientificas, Apartado 18, 03550 Sant Joan d Alacant, Alicante, Spain
J Biol Chem 280:6642-7. 2005..Rather, a network of interactions formed by residues Lys-46, Asp-128, Asp-135, Asp-266, and possibly others appears to link agonist binding to channel gating...
- Geometry of nonbonded interactions involving planar groups in proteinsPinak Chakrabarti
Department of Biochemistry and Bioinformatics Centre, Bose Institute, P 1 12 CIT Scheme VIIM, Kolkata 700054, India
Prog Biophys Mol Biol 95:83-137. 2007..The geometrical relationship between interacting residues provides valuable insights into the process of protein folding and would be useful for the design of protein molecules and modulation of their binding properties...
- Insights into the structure and molecular basis of ligand docking to the G protein-coupled secretin receptor using charge-modified amino-terminal agonist probesMaoqing Dong
M D Director, Cancer Center Mayo Clinic in Scottsdale, 13400 East Shea Boulevard, Johnson Research Building, Scottsdale AZ 85259, USA
Mol Endocrinol 19:1821-36. 2005..This supports the possibility that there is a charge-charge interaction between this residue and the amino terminus of secretin that is critical to its normal docking...
- NMR solution structure of the archaebacterial chromosomal protein MC1 reveals a new protein foldFrançoise Paquet
Centre de Biophysique Moleculaire, CNRS, rue Charles Sadron, 45071 Orleans Cedex 2, France
Biochemistry 43:14971-8. 2004..Its structure suggests a different DNA-binding mode than those of the histone-like proteins HU or HMGB. Thus, MC1 may be classified as a member of a new family...
- Possible association of non-binding of HSP70 to HLA-DRB1 peptide sequences and protection from rheumatoid arthritisJan T Maier
Department of Hematology and Oncology, Children's Hospital, , Hoppe-Seyler-Strasse 1, , Germany
Immunogenetics 54:67-73. 2002..Thus, our results suggest a possible association of non-binding of HSP70 to HLA-DR molecules or its 70-74 fragments and protection from RA...
- Cooperative interactions between R531 and acidic residues in the voltage sensing module of hERG1 channelsDavid R Piper
Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, UT 84113, USA
Cell Physiol Biochem 21:37-46. 2008..We propose that hERG1 activation involves a cooperative conformational change involving the entire voltage sensing module, while inactivation may involve a more limited interaction between R531 and D456, D460 and D509...
- Characterization and location of post-translational modifications on chromogranin B from bovine adrenal medullary chromaffin granulesClaire Gasnier
, , Strasbourg, France
Proteomics 4:1789-801. 2004..Furthermore, we have identified at the molecular level the presence of a mutation M/V on position 577 of natural CGB. All together these data reflect the complex structure of this protein marker of the neuroendocrine system...
- Identification and mutational analysis of Mg2+ binding site in EcoP15I DNA methyltransferase: involvement in target base eversionPradeep Bist
Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India
J Biol Chem 278:41837-48. 2003..This is the first example of metal-dependent function of a DNA methyltransferase. These findings provide impetus for exploring the role(s) of metal ions in the structure and function of DNA methyltransferases...
- The cytoplasmic domain of the Plasmodium falciparum ligand EBA-175 is essential for invasion but not protein traffickingTim-Wolf Gilberger
The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
J Cell Biol 162:317-27. 2003..These results show that the parasite uses the same components of its cellular machinery for invasion regardless of the host cell type and invasive form...
- Sequence context strongly modulates association of polar residues in transmembrane helicesJessica P Dawson
Department of Molecular Biophysics and Biochemistry, Yale University, Bass Centre Room 420A, 266 Whitney Avenue, New Haven, CT 06520-8114, USA
J Mol Biol 331:255-62. 2003..Overall, these results suggest that an innate structural mechanism may operate to control non-specific association of membrane-embedded polar residues...
- The luteinizing hormone receptor: influence of buffer composition on ligand binding and signaling of wild type and mutant receptorsKrassimira Angelova
Department of Biochemistry and Molecular Biology, University of Georgia, Life Sciences Building, Green Street, Athens, GA 30602 7229, USA
Mol Cell Endocrinol 204:1-9. 2003....
- A ring of eight conserved negatively charged amino acids doubles the conductance of BK channels and prevents inward rectificationTinatin I Brelidze
Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 N.W. 10th Avenue, Miami, FL 33136, USA
Proc Natl Acad Sci U S A 100:9017-22. 2003..Removing the ring of charge converts BK channels to inwardly rectifying channels. Thus, a simple electrostatic mechanism contributes to the large conductance of BK channels...
- The parsley plastocyanin-turnip cytochrome f complex: a structurally distorted but kinetically functional acidic patchPeter B Crowley
, Universidade Nova de Lisboa, , Apartado 127, 2781 901 Oeiras, Portugal
Biochem J 378:45-51. 2004..The results of NMR studies and protein-docking simulations indicate that parsley PCu and its spinach analogue adopt different orientations in their complexes with cyt f...
- The mouse and human Ah receptor differ in recognition of LXXLL motifsColin Flaveny
Center for Molecular Toxicology and Carcinogenesis, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, 309A Life Sciences Building, University Park, PA 16802, USA
Arch Biochem Biophys 471:215-23. 2008..These findings suggest that the human and mouse AhR may display differential recruitment of coactivators and hence may exhibit divergent regulation of target genes...
- Characterization of calcium binding properties of lithostathineByung-In Lee
Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA
J Biol Inorg Chem 8:341-7. 2003....
- A class of novel nitronyl nitroxide labeling basic and acidic amino acids: synthesis, application for preparing ESR optionally labeling peptides, and bioactivity investigationsJianwei Zhang
College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China
Bioorg Med Chem 16:4019-28. 2008..Their incorporation led to 12 novel ESR optionally labeling PAK, RGDS, RGDV, and ECG. A series of NO related chemical tests, the in vitro and in vivo assays of these peptides confirmed that this strategy was practical...
- Mutation of charged residues in the TR3 death domain does not perturb interaction with TRADDElisabeth Tunbridge
Psychiatry Centre of Excellence for Drug Discovery, GlaxoSmithKline, Third Avenue, Harlow, EssexCM19 5AW, UK
Protein Eng 15:811-5. 2002..These data suggest that despite some similarity between the death domains of TR3 and TNFR1 the nature of the interaction with TRADD differs from that reported for TNFR1...
- Immunoglobulin kappa light chain and its amyloidogenic mutants: a molecular dynamics studyMateusz Nowak
Department of Biostatistics and Medical Informatics, Collegium Medicum, and Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
Proteins 55:11-21. 2004..This suggests that mild and slow destabilization is the reason of the conversion of V(L) to partially folded amyloidogenic intermediate structure...
- Redox potential difference between Desulfovibrio vulgaris and Clostridium beijerinckii flavodoxinsHiroshi Ishikita
Department of Chemistry, University of Southern California, Los Angeles, California 90089, USA
Biochemistry 47:4394-402. 2008..The E sq/hq difference can be best explained by the lower compensation of the side chain influence by the backbone influence in the D. vulgaris flavodoxin than in the C. beijerinckii flavodoxin...
- Both acidic and basic amino acids in an amphitropic enzyme, CTP:phosphocholine cytidylyltransferase, dictate its selectivity for anionic membranesJoanne E Johnson
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
J Biol Chem 278:514-22. 2003..Thus anionic lipids, working via surface-localized pH effects, can promote membrane binding by modifying protein charge and hydrophobicity, and this novel mechanism contributes to the membrane selectivity of CT in vivo...
- MAP2 prevents protein aggregation and facilitates reactivation of unfolded enzymesTaradas Sarkar
Department of Biochemistry, Centenary Campus, PI/12, Bose Institute, CIT Scheme VIIM, Calcutta 700-054, India
Eur J Biochem 271:1488-96. 2004..Tau, which shares a homologous C-terminal domain with MAP2, possesses no such activity...
- Interactions between neuronal fusion proteins explored by molecular dynamicsMarie Pierre Durrieu
Institut de Biologie Physico Chimique, Laboratoire de Biochimie Theorique, Centre National de la Recherche Scientifique, UPR 9080, Paris, France
Biophys J 94:3436-46. 2008..The interaction patterns we observe provide a new tool for interpreting the impact of mutations on the complex...
- Are acidic and basic groups in buried proteins predicted to be ionized?Jinrang Kim
Physics Department J 419, City College of New York, 138th Street and Convent Avenue, New York, NY 10031, USA
J Mol Biol 348:1283-98. 2005..Thus, hydroxyls are better hydrogen bond donors than acceptors. Buried ionized residues are more likely to be conserved than those on the surface. There are 3.95 residues buried per 100 residues in an average protein...
- Ion-ion and ion-molecule reactions at the surface of proteins produced by nanospray. Information on the number of acidic residues and control of the number of ionized acidic and basic residuesUdo H Verkerk
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
J Am Soc Mass Spectrom 16:1325-41. 2005....
- Calcium regulation of inositol 1,4,5-trisphosphate receptorsSuresh K Joseph
Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Rm 230A JAH, 1020 Locust Street, Philadelphia, PA 19107, USA
Cell Calcium 38:539-46. 2005..The data support the view that all IP3R isoforms may display a range of Ca2+ sensitivities that are determined by multiple sites within the protein and markedly influenced by the affinity of the receptor for IP3...
- Hydrophobic and acidic moments of a nucleoplasmin NP-core chaperoneB David Silverman
IBM Thomas J Watson Research Center, Yorktown Heights, NY 10598, USA
J Biomol Struct Dyn 24:49-56. 2006..It is also pointed out that the prevalence of basic residues on the short helices of the histone cores also provides regions of charge that would complement histone binding to the chaperone...
- Effects of hydrogen-bonding and stacking interactions with amino acids on the acidity of uracilKen C Hunter
Department of Chemistry, Mount Allison University, 63C York Street, Sackville, New Brunswick E4L 1G8, Canada
J Phys Chem B 111:1858-71. 2007..The present study has general implications for understanding the nature of active site amino acids in enzymes, such as DNA repair enzymes, that catalyze reactions involving anionic nucleobase intermediates...
- Structural basis for budding by the ESCRT-III factor CHMP3Tadeusz Muzioł
European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
Dev Cell 10:821-30. 2006..Thus, the final steps in the budding process may include CHMP protein polymerization and lattice formation on membranes by employing different bilayer-recognizing surfaces, a function shared by all CHMP family members...
- The alpha-helix of the second chromodomain of the 43 kDa subunit of the chloroplast signal recognition particle facilitates binding to the 54 kDa subunitRebecca Hermkes
, , 44780 Bochum, Germany
FEBS Lett 580:3107-11. 2006..Further analysis of the binding interface of cpSRP by mutagenesis studies and a pepscan approach demonstrates that the C-terminal alpha-helix of CD2 facilitates binding to cpSRP54...
- Role of acidic amino acid residues in chitooligosaccharide-binding to Streptomyces sp. N174 chitosanaseTomomi Katsumi
Department of Advanced Bioscience, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan
Biochem Biophys Res Commun 338:1839-44. 2005..The fluorescence quenching was very weak in D57A, suggesting a strong contribution of this residue to the oligosaccharide binding...
- Cell surface adhesion of pregnancy-associated plasma protein-A is mediated by four clusters of basic residues located in its third and fourth CCP moduleKathrin Weyer
Department of Molecular Biology, Science Park, University of Aarhus, Gustav Wieds Vej 10C, DK-8000 Aarhus C, Denmark
Eur J Biochem 271:1525-35. 2004..This explains, at least in part, why PAPP-A2 lacks the ability of cell surface adhesion, and further emphasizes the role of the basic clusters defined in PAPP-A...
- Tools for building a comprehensive modeling system for virtual screening under real biological conditions: The Computational Titration algorithmGlen E Kellogg
Department of Medicinal Chemistry and Institute for Structural Biology and Drug Discovery, School of Pharmacy, Virginia Commonwealth University, Box 980540, Richmond, VA 23298 0540, USA
J Mol Graph Model 24:434-9. 2006..As an example, three complexes of dihydrofolate reductase (DHFR) are analyzed with our system and these results are compared with the experimental free energies of binding...
- pH sensing in the two-pore domain K+ channel, TASK2Michael J Morton
School of Biomedical Sciences, Medical and Dental Building, University of Leeds, Leeds LS2 9NQ, United Kingdom
Proc Natl Acad Sci U S A 102:16102-6. 2005..Thus, pH sensing in TASK2 channels is conferred by the combined action of several charged residues in the large extracellular M1-P1 loop...
- Chemiluminescent studies on the antioxidant activity of amino acidsT M Triantis
Institute of Physical Chemistry, NCSR Demokritos, 15310 Ag Paraskevi, Athens, Greece
Anal Chim Acta 591:106-11. 2007..Amino acids with phenolic groups present higher inhibition than basic, acidic or neutral amino acids. The calculated antioxidant activities by the CL method are compared with those of the N,N-diphenyl-picryl hydrazyl (DPPH) method...
- Towards control of aggregational behaviour of alpha-lactalbumin at acidic pHJane B Pedersen
Institute of Physics and Nanotechnology, Aalborg University, Aalborg, Denmark
J Fluoresc 16:611-21. 2006..The results presented in this paper provide detailed knowledge on the external parameters leading to aggregation of alpha-La at acidic pH, thus permitting rational design of the aggregation process...
- Non-covalent interactions between unsolvated peptides: helical complexes based on acid-base interactionsRajagopalan Sudha
Chemistry Department, Indiana University, Bloomington, Indiana 47405-7102, USA
J Phys Chem B 109:6442-7. 2005..The enthalpy change indicates that the interaction between the E and K residues in the middle of the helices is a weak neutral hydrogen bond. The EKK interaction is significantly stronger...
- Ammonium assimilation and amino acid metabolism in conifersFrancisco M Canovas
Departamento de Biologia Molecular y Bioquimica, Instituto Andaluz de Biotecnología, Unidad Asociada UMA CSIC, Universidad de Malaga, E 29071 Malaga, Spain
J Exp Bot 58:2307-18. 2007..In this paper, details of recent progress in our understanding of the metabolism of arginine and the other major amino acids glutamine, glutamate, aspartate, and asparagine in pine, a conifer model tree, are presented and discussed...
- Piv site-specific invertase requires a DEDD motif analogous to the catalytic center of the RuvC Holliday junction resolvasesJohn M Buchner
Department of Microbiology, University of Georgia, Athens, GA 30602-2605, USA
J Bacteriol 187:3431-7. 2005..On the basis of these results, a working model for Piv-mediated inversion that includes resolution of a Holliday junction is presented...
- The acidic repetitive domain of the Magnetospirillum gryphiswaldense MamJ protein displays hypervariability but is not required for magnetosome chain assemblyAndré Scheffel
Max Planck Institute for Marine Microbiology, Bremen, Germany
J Bacteriol 189:6437-46. 2007....
- Dph3, a small protein required for diphthamide biosynthesis, is essential in mouse developmentShihui Liu
Bacterial Toxins and Therapeutics Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Biol 26:3835-41. 2006..These results attest to the physiological importance of Dph3 in development. The biological roles of Dph3 are also discussed...
- A di-acidic sequence motif enhances the surface expression of the potassium channel TASK-3Marylou Zuzarte
Institute of Physiology, Marburg University, Deutschhausstr 2, 35037 Marburg, Germany
Traffic 8:1093-100. 2007..Our data suggest that the di-acidic motif, EDE, in human TASK-3 is a major determinant of the rate of ER export and is required for efficient surface expression of the channel...
- Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acidsWolf N Fischer
Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Tubingen, Germany
Plant J 29:717-31. 2002Amides and acidic amino acids represent the major long distance transport forms of organic nitrogen. Six amino acid permeases (AAPs) from Arabidopsis mediating transport of a wide spectrum of amino acids were isolated...
- Phosphorylation of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein, by casein kinase IIJ A Girault
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021
J Biol Chem 264:21748-59. 1989..2-fold increase in the Vmax and a 1.4-fold increase in the apparent Km. Phosphorylation of DARPP-32 by casein kinase II in intact cells may therefore modulate its phosphorylation in response to increased levels of cAMP...
- Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98)Y Kanai
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181, Japan
J Biol Chem 273:23629-32. 1998..neutral amino acids with branched or aromatic side chains and did not accept basic amino acids or acidic amino acids. The transport via LAT1 was Na+-independent and sensitive to a system L-specific inhibitor 2-aminobicyclo-(2,..
- Rat kidney carboxylesterase. Cloning, sequencing, cellular localization, and relationship to rat liver hydrolaseB Yan
Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City 66160 7417
J Biol Chem 269:29688-96. 1994....
- A two-alpha-helix extra domain mediates the halophilic character of a plant-type ferredoxin from halophilic archaeaBianca Lucia Marg
Max Planck Institut fur Biochemie, Abteilung Membranbiochemie, Am Klopferspitz 18a, 82152 Martinsried, Germany
Biochemistry 44:29-39. 2005..ferredoxins except for an insertion of 30 amino acids near its N-terminus which is extremely rich in acidic amino acids. Unfolding studies reveal that HsFdx has an unfolding temperature of approximately 85 degrees C in 4...
- LSA: a new liver-specific antigen in the rat. I. Purification and characterizationA A Mihas
Division of Digestive Diseases, University of Mississippi School of Medicine, Jackson
Int Arch Allergy Immunol 98:35-49. 1992..The amino acid composition of the LSA revealed that the acidic amino acids out-number the basic amino acids, with glutamic acid being the most abundant of them...
- A-type ATP binding consensus sequences are critical for the catalytic activity of the calmodulin-sensitive adenylyl cyclase from Bacillus anthracisZ G Xia
Department of Pharmacology, School of Medicine, University of Washington, Seattle 98195
J Biol Chem 265:6517-20. 1990..It is concluded that Lys-346 and Lys-353 in B. anthracis adenylyl cyclase may interact directly with ATP and contribute to the binding of the nucleotide to the enzyme...
- Unique dicistronic operon (ptsI-crr) in Mycoplasma capricolum encoding enzyme I and the glucose-specific enzyme IIA of the phosphoenolpyruvate:sugar phosphotransferase system: cloning, sequencing, promoter analysis, and protein characterizationP P Zhu
Laboratory of Biochemical Genetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
Protein Sci 3:2115-28. 1994..amino acid sequence of the Mycoplasma Enzyme I shows that it differs from other Enzymes I by having fewer acidic amino acids and more basic, amidated, and aromatic amino acids...
- Replacements of single basic amino acids in the pleckstrin homology domain of phospholipase C-delta1 alter the ligand binding, phospholipase activity, and interaction with the plasma membraneH Yagisawa
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Garden City, Hyogo 678 12, Japan
J Biol Chem 273:417-24. 1998..All residues (with the exception of the K43E substitution) found to be critical for the analyzed PLC-delta1 functions are present at the surface of the PH domain shown to contain the Ins(1,4,5)P3 binding pocket...
- Electron transfer in Paracoccus denitrificans with the modified fbc operonV Dadak
Institute of Biochemistry, Faculty of Science, Masaryk University, Brno, Czechia
Folia Microbiol (Praha) 54:475-82. 2009..The mutant strain M 71 expressing the truncated copy of cytochrome c(1) (devoid of a stretch of 150 mainly acidic amino acids) was less sensitive to increasing concentration of cytochrome c and changes in ionic strength of the medium, ..
- Phosphorylation of the angiotensin II (AT1A) receptor carboxyl terminus: a role in receptor endocytosisW G Thomas
Baker Medical Research Institute, Melbourne, Australia
Mol Endocrinol 12:1513-24. 1998..sites (Thr332, Ser335, Thr336, and Ser338) were substituted with either neutral [alanine (A)] or acidic amino acids [glutamic acid (E) and aspartic acid (D)], the former to prevent phosphorylation and the latter to reproduce ..
- Involvement of phylogenetically conserved acidic amino acid residues in catalysis by an oxidative DNA damage enzyme formamidopyrimidine glycosylaseO V Lavrukhin
Sealy Center for Molecular Science, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1071, USA
Biochemistry 39:15266-71. 2000..However, in contrast to other enzymes in this class, in which acidic amino acids have been shown to be essential for glycosyl and phosphodiester bond scission, the catalytically essential ..
- Electrostatic attraction between cytochrome bc1 and cytochrome c affects kinetics of cytochrome c reductionV Dadak
Institute of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
Biochemistry (Mosc) 73:870-80. 2008..The cytochrome c(1) of the WT samples possessed an additional stretch of acidic amino acids, which was lacking in the mutant...
- Molecular cloning of a histidine-rich Ca2+-binding protein of sarcoplasmic reticulum that contains highly conserved repeated elementsS L Hofmann
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235
J Biol Chem 264:18083-90. 1989..tandem repeats of 29 residues, each consisting of a histidine-rich sequence HRHRGH, a stretch of 10-11 acidic amino acids, and a sequence containing 2 serines and a threonine in a negatively charged context; 3) a 13-residue ..
- Homology models of the Yersinia pseudotuberculosis and Yersinia pestis general porins and comparative analysis of their functional and antigenic regionsG N Likhatskaya
Pacific Institute of Bioorganic Chemistry of Far East Branch of Russian Academy of Sciences, Vladivostok, Pr 100 Let Vladivostoku 159, 690022 Russia
J Biomol Struct Dyn 23:163-74. 2005..located in the loop L3 tip that disturbs the functionally important cluster of the acidic amino acids in the constriction site. Comparative analysis of structural organization of YPS and E...
- Contribution of amino acid region 659-663 of Factor Va heavy chain to the activity of factor Xa within prothrombinase JAMILA HIRBAWI
Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
Biochemistry 49:8520-34. 2010....
- Identification of a novel Na+-independent acidic amino acid transporter with structural similarity to the member of a heterodimeric amino acid transporter family associated with unknown heavy chainsHirotaka Matsuo
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
J Biol Chem 277:21017-26. 2002..proteins were sorted to the plasma membrane and expressed the Na(+)-independent transport activity for acidic amino acids. Distinct from the Na(+)-independent cystine/glutamate transporter xCT structurally related to AGT1, AGT1 ..
- The computer modelling of human TRH receptor, TRH and TRH-like peptidesR Bilek
Institute of Endocrinology, First Faculty of Medicine, Charles University, Narodni 8, 11694 Prague 1, Cech Republic
Physiol Res 54:141-50. 2005..Desamido-TRH or glutamyl metabolites will be repelled by the negative potential of ASP195 (E: D93) and GLU298 (G: E137)...
- Evaluation of the RBE4 cell line to explore carrier-mediated drug delivery to the CNS via the L-system amino acid transporter at the blood-brain barrierAndreas Reichel
Blood Brain Barrier Research Group, Centre for Neuroscience Research, GKT School of Biomedical Sciences, King s College London, UK
J Drug Target 10:277-83. 2002..such as L-phenylalanine and 2-aminobicyclo-heptanecarboxylic acid (BCH), whereas small neutral, basic and acidic amino acids had no significant effect...
- cDNA cloning of a novel protein containing two zinc-finger domains that may function as a transcription factor for the human heme-oxygenase-1 geneY Muraosa
Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine, Sendai, Japan
Eur J Biochem 235:471-9. 1996..possesses other structural features characteristic of transcription factors, including a long stretch of acidic amino acids (amino acids 67 - 95), a proline-rich region (positions 733-849), a region rich in basic amino acids (..
- The c-erbA alpha-encoded thyroid hormone receptor is phosphorylated in its amino terminal domain by casein kinase IIC Glineur
INSERM U186 CNRS UA 1160, Institut Pasteur, Lille, France
Oncogene 4:1247-54. 1989....
- Herniated lumbar disc material as a source of free glutamate available to affect pain signals through the dorsal root ganglionJ F Harrington
Brown University School of Medicine and the Rhode Island Hospital, Providence, Rhode Island, USA
Spine (Phila Pa 1976) 25:929-36. 2000..Combined prospective human cohort and prospective controlled animal model...
- Aspartic acid 405 contributes to the substrate specificity of aminopeptidase BKayoko M Fukasawa
Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, School of Dentistry, Shiojiri, Nagano 399 0781, Japan
Biochemistry 45:11425-31. 2006..These data strongly suggest that the Asp 405 residue is involved in substrate binding via an interaction with the P1 amino group of the substrate's side chain...
- Molecular cloning and characterization of a cellular protein that interacts with the human immunodeficiency virus type 1 Tat transactivator and encodes a strong transcriptional activation domainL Yu
Institute for Molecular Virology, Saint Louis University School of Medicine, Missouri 63110, USA
J Virol 69:3007-16. 1995..By deletion analysis, we have identified a TAP C-terminal region rich in acidic amino acids and leucine residues which acts as a strong transcriptional activator when bound through GAL4 sites upstream ..
- Mutation effects of a conserved alanine (Ala510) in type I polyhydroxyalkanoate synthase from Ralstonia eutropha on polyester biosynthesisTakeharu Tsuge
Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama, Kanagawa 226 8502, Japan
Macromol Biosci 4:963-70. 2004..In particular, substitution by acidic amino acids, A510D(E), led to remarkable increases in molecular weights...
- Modulators of the activation of the proteasome by PA28 (11S reg)S Wilk
Department of Pharmacology, Mount Sinai School of Medicine, New York, NY 10029, USA
Mol Biol Rep 26:39-44. 1999..PA28- proteasome modulators by modifying the profile of peptides produced by the activated proteasome, may either enhance or suppress the immune response...
- Caspase-dependent N-terminal cleavage of influenza virus nucleocapsid protein in infected cellsO P Zhirnov
D I Ivanovsky Institute of Virology, 123098 Moscow, Russia
J Virol 73:10158-63. 1999..With D at position -1, acidic amino acids at position -3, and aliphatic ones at positions -2 and +1, the NP cleavage sites show a recognition motif ..
- Genes for a second terminal oxidase in Bradyrhizobium japonicumM Bott
Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
Arch Microbiol 158:335-43. 1992..This shows that the coxMNOP genes are not essential for respiration in the N2 fixing bacteroid...
- Comparative inhibition by hard and soft metal ions of steroid-binding capacity of renal mineralocorticoid receptor cross-linked to the 90-kDa heat-shock protein heterocomplexM D Galigniana
Departamento de Qu approximately ímica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, and PHROM CONICET, 1428 Buenos Aires, Argentina
Biochem J 341:585-92. 1999..chemical modification of cysteines with dithionitrobenzoic acid, histidines with diethyl pyrocarbonate and acidic amino acids with Woodward's reagent (N-ethyl-5-phenylisoxazolium-3'-sulphonate)...
- Select nutrients and their associated transporters are increased in the ovine uterus following early progesterone administrationM Carey Satterfield
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station, Texas 77843 2471, USA
Biol Reprod 82:224-31. 2010..Thus, select nutrients are increased in the uterine lumen by P4 concomitant with the upregulation of epithelial transporters for glucose and basic amino acids, suggesting that these nutrients stimulate blastocyst growth and development...
- Characterization of a rat Na+-dicarboxylate cotransporterX Z Chen
Renal Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 273:20972-81. 1998..Of note, the acidic amino acids L- and D-glutamate and aspartate were also transported, although with lower affinity (Km = 2-18 mM)...
- Normal expression and the effects of ectopic expression of the Drosophila muscle segment homeobox (msh) gene suggest a role in differentiation and patterning of embryonic musclesP C Lord
Department of Biochemistry M C 536, University of Illinois, College of Medicine, Chicago 60612, USA
Dev Biol 171:627-40. 1995..spiracles gene and encodes several stretches of amino acids rich in serine, alanine, proline, glutamine, and acidic amino acids, indicating potential domains of regulatory activity...
- Aspartate-444 is essential for productive substrate interactions in a neuronal glutamate transporterShlomit Teichman
Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
J Gen Physiol 129:527-39. 2007..In contrast to acidic amino acids, dicarboxylic acids are not recognized by glutamate transporters, but the related bacterial DctA ..
- Influence of an anion-binding site in the stabilization of halophilic malate dehydrogenase from Haloarcula marismortuiDominique Madern
Institut de Biologie Structurale Jean Pierre Ebel, UMR 5075, CEA, CNRS, Universite Joseph Fourier, 41 rue Jules Horowitz, F 38027 Grenoble, France
Biochimie 89:981-7. 2007..Crystallographic studies have shown that surface enrichment by acidic amino acids is a common structural feature of halophilic proteins...
- Purification and biochemical characterization of a putative [6Fe-6S] prismane-cluster-containing protein from Desulfovibrio vulgaris (Hildenborough)A J Pierik
Department of Biochemistry, Agricultural University, Wageningen, The Netherlands
Eur J Biochem 206:697-704. 1992..vulgaris (Monticello) and Desulfovibrio desulfuricans (ATCC 27774). We have not yet identified a physiological function for the prismane protein despite trials for some relevant enzyme activities...
- Identification of acidic and aromatic residues in the Zta activation domain essential for Epstein-Barr virus reactivationZ Deng
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Virol 75:10334-47. 2001....
- Acidic amino acids flanking phosphorylation sites in the M2 muscarinic receptor regulate receptor phosphorylation, internalization, and interaction with arrestinsK B Lee
Department of Molecular Pharmacology and Biological Chemistry, The Institute for Neuroscience, Northwestern University Medical School, Evanston, Illinois 60208, USA
J Biol Chem 275:35767-77. 2000..L., and Hosey, M. M. (1997) J. Biol. Chem. 272, 23682-26389). The present study investigated the role of acidic amino acids between P1 and P2 in regulating receptor phosphorylation, internalization, and receptor/arrestin ..
- Systematic Analysis of proteoglycan modification sites in Caenorhabditis elegans by scanning mutagenesisHuan Wang
Department of Biochemistry and Biophysics, Center for Oral Biology, Aab Institute of Biomedical Sciences, University of Rochester Medical Center, Rochester, NY 14642, USA
J Biol Chem 282:14586-97. 2007..either one proximal acidic N-terminal amino acid (positions -4, -3, and -2) or a pair of distal N-terminal acidic amino acids (positions -6 and -5)...
- Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 1T M Rizzo
Department of Plant Pathology, Cornell University, Ithaca, New York 14853
J Gen Virol 70:1-11. 1989..While the C-terminal 141 amino acids contain more basic than acidic amino acids, the region of greatest amino acid sequence heterogeneity, amino acids 503 to 600, contained a preponderance ..
- Role of the HCF-1 basic region in sustaining cell proliferationMarco Mangone
Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America
PLoS ONE 5:e9020. 2010..b>acidic amino acids) that is required for cell proliferation and has been shown to associate with the Sin3 histone deacetylase (..
- Two clusters of acidic amino acids near the NH2 terminus of complement component C4 alpha'-chain are important for C2 bindingQ Pan
Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
J Immunol 165:2518-27. 2000..Taken together, these results strongly suggest that the NH2-terminal acidic residue-rich segment of C4 alpha'-chain contributes importantly to the interaction of C4b with C2...
- Rat kidney glutamyl aminopeptidase (aminopeptidase A): molecular identity and cellular localizationL Song
Department of Pharmacology, Mount Sinai School of Medicine, City University of New York, New York 10029
Am J Physiol 267:F546-57. 1994....
- TRH-like peptides in prostate gland and other tissuesR Bilek
Institute of Endocrinology, Prague, Czech Republic
Physiol Res 49:S19-26. 2000..characterized by substitution of the basic amino acid histidine (related to authentic TRH) for neutral or acidic amino acids, such as glutamic acid, phenylalanine, glutamine or tyrosine...
- An aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8A Okamoto
Department of Biology, Faculty of Science, Osaka University, Toyonaka
J Biochem 119:135-44. 1996..Despite the high degree of conservation of active-site residues, Arg292 in E. coli AspAT, which interacts with the distal carboxylate of the substrate, was not found in T. thermophilus AspAT. Arg89 may complement the function of Arg292...
- Mutation at a single acidic amino acid enhances the halophilic behaviour of malate dehydrogenase from Haloarcula marismortui in physiological saltsD Madern
Institut de Biologie Structurale, Grenoble, France
Eur J Biochem 230:1088-95. 1995In a statistical analysis of the amino acid compositions of 26 halophilic proteins, 24 showed an increase in acidic amino acids and a decrease in basic ones when compared to their non-halophilic homologues...
- Influence of the magnesium aspartate hydrochloride administration to the maternal circuit on the aspartate concentration of the fetal circuit under in vitro perfusion of human placentaA Malek
Department of Obstetrics and Gynecology, University of Berne, Inselspital, Switzerland
Eur J Obstet Gynecol Reprod Biol 142:12-7. 2009..Animal experiments have demonstrated that highly increased fetal levels of acidic amino acids such as Asp could be associated with neurotoxic damage in the fetal brain...
- Development of Chemical Receptors for ProteinsSanku Mallik; Fiscal Year: 2005..Testing of the synthetic receptors for possible applications in detection of metastatic cancer, imaging and delivery of anti- cancer drugs. ..
- Tyrosine Sulfation and Chemokine Receptor FunctionKevin Moore; Fiscal Year: 2006..domains of all known chemokine receptors (CCRs) contain tyrosine residues in the context of several flanking acidic amino acids, features shared by all known tyrosine sulfated proteins...
- FUNCTIONAL SIGNIFICANCE OF THE ACTIN N-TERMINAL REGIONPETER RUBENSTEIN; Fiscal Year: 1993..grant addresses the importance of three conserved elements of the 18 amino acid actin N-terminal region: the acidic amino acids at the N-terminus of mature processed actin; the conserved Ala-Leu-Val beginning at residue 6; and the ..
- Factor Va regulation of prothrombinase activityJAMILA HIRBAWI; Fiscal Year: 2006..Proteins will be screened for clotting activity in one-and two-stage clotting assays. Kinetic analyses will be done by measuring thrombin formation. ..
- GLUTAMATE TRANSPORTERS IN THE CNSMichael Robinson; Fiscal Year: 2009..The name of the principal investigator/program director must be provided at the top of each printed page and each continuation page. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page i_ Description, ..
- MECHANISM OF INOSITOL TRISPHOSPHATE ACTIONSuresh Joseph; Fiscal Year: 2004..Ca2+ is the principal regulator of IP3R and exerts a biphasic effect on channel function. Acidic amino acids in 2 known calcium-binding regions of the IP3R will be mutated...
- GLUTAMATE TRANSPORTERS IN THE CNSMICHAEL BYRNE ROBINSON; Fiscal Year: 2011..The name of the principal investigator/program director must be provided at the top of each printed page and each continuation page. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page i_ Description, ..
- REACTIVE INTERMEDIATES OF ENZYMATIC REACTIONSJohn Richard; Fiscal Year: 2000....
- BRAIN ENDOPEPTIDASES AND NEUROPEPTIDE METABOLISMSherwin Wilk; Fiscal Year: 2001....
- REGULATION OF EXTRACELLULAR EXCITOTOXINSMichael Robinson; Fiscal Year: 2006The acidic amino acids, glutamate and aspartate, are the predominant excitatory neurotransmitters in the mammalian CNS. An extracellular accumulation of these excitatory amino acids (EAAs) causes neuronal cell death...
- GLUTAMATE TRANSPORTERS IN THE CNSMichael Robinson; Fiscal Year: 2001DESCRIPTION: The acidic amino acids are the predominant excitatory neurotransmitters in the mammalian central nervous system (CNS)...