pleckstrin homology domains


Summary: Protein domains of approximately 120 amino acids that form two perpendicular anti-parallel beta sheets connected by a loop of variable length and a C-terminal amphipathic helix. PH domains occur in many INTRACELLULAR SIGNALING PROTEINS and CYTOSKELETAL PROTEINS where they bind PHOSPHATIDYLINOSITOLS within the CELL MEMBRANE and INTRACELLULAR MEMBRANES, as well as proteins that include the Betagamma-subunits of HETEROTRIMERIC GTP-BINDING PROTEINS, and PROTEIN KINASE C. These interactions allow the targeting of proteins to different cellular compartments and SIGNAL TRANSDUCTION PATHWAYS.

Top Publications

  1. Zhu S, Khatun R, Lento C, Sheng Y, Wilson D. Enhanced Binding Affinity via Destabilization of the Unbound State: A Millisecond Hydrogen-Deuterium Exchange Study of the Interaction between p53 and a Pleckstrin Homology Domain. Biochemistry. 2017;56:4127-4133 pubmed publisher
  2. Bradshaw N, Yerabham A, Marreiros R, Zhang T, Nagel Steger L, Korth C. An unpredicted aggregation-critical region of the actin-polymerizing protein TRIOBP-1/Tara, determined by elucidation of its domain structure. J Biol Chem. 2017;292:9583-9598 pubmed publisher
  3. Devkota S, Joseph R, Boyken S, Fulton D, Andreotti A. An Autoinhibitory Role for the Pleckstrin Homology Domain of Interleukin-2-Inducible Tyrosine Kinase and Its Interplay with Canonical Phospholipid Recognition. Biochemistry. 2017;56:2938-2949 pubmed publisher
  4. Zhang J, Dubey P, Padarti A, Zhang A, Patel R, Patel V, et al. Novel functions of CCM1 delimit the relationship of PTB/PH domains. Biochim Biophys Acta Proteins Proteom. 2017;1865:1274-1286 pubmed publisher
    ..The study provides new insight into the structural characteristics of PTB/PH domains, essential structural elements of PTB/PH domain required for NPXY motif-binding, and function and relationship among PTB, PH and FERM domains. ..
  5. Xu Z, Gakhar L, Bain F, Spies M, Fuentes E. The Tiam1 guanine nucleotide exchange factor is auto-inhibited by its pleckstrin homology coiled-coil extension domain. J Biol Chem. 2017;292:17777-17793 pubmed publisher
    ..indicate that larger fragments of Tiam1, such as the region encompassing the N-terminal to C-terminal pleckstrin homology domains (PHn-PHc), are auto-inhibited...