protein refolding


Summary: Conformational transitions of a protein from unfolded states to a more folded state.

Top Publications

  1. Mahmoud M, Schechter A, Alnajjar K, Huang J, Towle Weicksel J, Eckenroth B, et al. Defective Nucleotide Release by DNA Polymerase β Mutator Variant E288K Is the Basis of Its Low Fidelity. Biochemistry. 2017;56:5550-5559 pubmed publisher
    ..Structural modeling implies that this inherent defect is due to interaction of E288K with DNA, resulting in a stable closed enzyme structure. ..
  2. Qiu Y, Levendosky R, Chakravarthy S, Patel A, Bowman G, Myong S. The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer. Mol Cell. 2017;68:76-88.e6 pubmed publisher
    ..We propose that active interplay of the ATPase motor with the regulatory domains may promote dynamic nucleosome structures uniquely suited for histone exchange and chromatin reorganization during transcription. ..
  3. Kaspersen J, Søndergaard A, Madsen D, Otzen D, Pedersen J. Refolding of SDS-Unfolded Proteins by Nonionic Surfactants. Biophys J. 2017;112:1609-1620 pubmed publisher
    ..We conclude that NIS competes with globular proteins for association with SDS, making it possible to release and refold SDS-denatured proteins by adding sufficient amounts of NIS, unless the protein also interacts with NIS alone. ..
  4. Li X, Anderson M, Collin D, Muegge I, Wan J, Brennan D, et al. Structural studies unravel the active conformation of apo ROR?t nuclear receptor and a common inverse agonism of two diverse classes of ROR?t inhibitors. J Biol Chem. 2017;292:11618-11630 pubmed publisher
  5. Serrano A, Lomont J, Tu L, Raleigh D, Zanni M. A Free Energy Barrier Caused by the Refolding of an Oligomeric Intermediate Controls the Lag Time of Amyloid Formation by hIAPP. J Am Chem Soc. 2017;139:16748-16758 pubmed publisher
    ..Our experiments and modeling provide evidence that protein structural rearrangements during aggregation impact the populations and kinetics of toxic oligomeric species. ..
  6. Rynkiewicz M, Wu H, Cafarella T, Nikolaidis N, Head J, Seaton B, et al. Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop. Biochemistry. 2017;56:4095-4105 pubmed publisher
    ..These studies suggest that flexibility in a key surface loop supports the distinctive lipid binding functions of SP-A, thus contributing to its multiple functions in surfactant structure and regulation, and host defense. ..
  7. Sánchez Trasviña C, Mayolo Deloisa K, González Valdez J, Rito Palomares M. Refolding of laccase from Trametes versicolor using aqueous two phase systems: Effect of different additives. J Chromatogr A. 2017;1507:25-31 pubmed publisher
    b>Protein refolding is a strategy used to obtain active forms of proteins from inclusion bodies...
  8. Stevens C, Rayani K, Singh G, Lotfalisalmasi B, Tieleman D, Tibbits G. Changes in the dynamics of the cardiac troponin C molecule explain the effects of Ca2+-sensitizing mutations. J Biol Chem. 2017;292:11915-11926 pubmed publisher
    ..Our data further suggest that modulation of the structural dynamics is the underlying molecular mechanism for many disease mutations that are far from the regulatory Ca2+-binding site of cTnC. ..
  9. Sontag E, Samant R, Frydman J. Mechanisms and Functions of Spatial Protein Quality Control. Annu Rev Biochem. 2017;86:97-122 pubmed publisher
    ..Spatial sequestration plays a central role in protein quality control and cellular fitness and represents a critical link to the pathogenesis of protein aggregation-linked diseases. ..

More Information


  1. Wang S, Borschel W, Heyman S, Hsu P, Nichols C. Conformational changes at cytoplasmic intersubunit interactions control Kir channel gating. J Biol Chem. 2017;292:10087-10096 pubmed publisher
    ..We conclude that the removal of stabilizing CD-I salt bridges results in a collapsed state of the Kir domain. ..
  2. Lewis J, Elder P. Monoclonal antibodies to the reactive centre loop (RCL) of human corticosteroid-binding globulin (CBG) can protect against proteolytic cleavage. J Steroid Biochem Mol Biol. 2017;171:247-253 pubmed publisher
    ..These findings may provide a basis and rationale for the concept of the use of RCL antibodies as therapeutic agents to effectively increase the proportion of intact CBG in circulation which may be of benefit in acute inflammation. ..
  3. Mitic S, Strampraad M, Hagen W, de Vries S. Microsecond time-scale kinetics of transient biochemical reactions. PLoS ONE. 2017;12:e0185888 pubmed publisher
    ..35 ?s for a total duration of ~600 ?s. As a proof of principle the instrument was used to study spontaneous protein refolding of pH-denatured cytochrome c...
  4. Schmidt J, Guillaume P, Dojcinovic D, Karbach J, Coukos G, Luescher I. In silico and cell-based analyses reveal strong divergence between prediction and observation of T-cell-recognized tumor antigen T-cell epitopes. J Biol Chem. 2017;292:11840-11849 pubmed publisher
    ..This observation highlights the need for improving in silico predictions of peptide immunogenicity. ..
  5. Mikula K, Tascón I, Tommila J, Iwai H. Segmental isotopic labeling of a single-domain globular protein without any refolding step by an asparaginyl endopeptidase. FEBS Lett. 2017;591:1285-1294 pubmed publisher
    ..This simple protein ligation approach using an AEP could be applied for incorporation of various biophysical probes into proteins as well as post-translational production of full-length proteins. ..
  6. Lv Z, Yuan L, Atkison J, Aldana Masangkay G, Chen Y, Olsen S. Domain alternation and active site remodeling are conserved structural features of ubiquitin E1. J Biol Chem. 2017;292:12089-12099 pubmed publisher
  7. Westerhold L, Bridges L, Shaikh S, Zeczycki T. Kinetic and Thermodynamic Analysis of Acetyl-CoA Activation of Staphylococcus aureus Pyruvate Carboxylase. Biochemistry. 2017;56:3492-3506 pubmed publisher
    ..Taken together, our kinetic and thermodynamic results provide a possible allosteric mechanism by which acetyl-CoA coordinates catalysis within the PC tetramer. ..
  8. Thakur A, Larimi M, Gooden K, Movileanu L. Aberrantly Large Single-Channel Conductance of Polyhistidine Arm-Containing Protein Nanopores. Biochemistry. 2017;56:4895-4905 pubmed publisher
  9. Pettersson J, Lanni F, Rule G. Dual Lifetimes for Complexes between Glutathione-S-transferase (hGSTA1-1) and Product-like Ligands Detected by Single-Molecule Fluorescence Imaging. Biochemistry. 2017;56:4073-4083 pubmed publisher
    ..Kinematic traces of fluorescence from single molecules show that a single molecule readily samples a number of different conformations, each with a characteristic off-rate. ..
  10. Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, et al. Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Nature. 2017;543:443-446 pubmed publisher
    ..We term this mitochondria-mediated proteostasis mechanism MAGIC (mitochondria as guardian in cytosol) and provide evidence that it may exist in human cells. ..
  11. Yao X, Cato M, Labudde E, Beyett T, Tesmer J, Grant B. Navigating the conformational landscape of G protein-coupled receptor kinases during allosteric activation. J Biol Chem. 2017;292:16032-16043 pubmed publisher
    ..Navigation of the conformational landscape defined by this bioinformatics-based study is likely common to all GPCR-activated GRKs. ..
  12. Pilipczuk J, Zalewska Piatek B, Bruzdziak P, Czub J, Wieczór M, Olszewski M, et al. Role of the disulfide bond in stabilizing and folding of the fimbrial protein DraE from uropathogenic Escherichia coli. J Biol Chem. 2017;292:16136-16149 pubmed publisher
    ..We also show that the disulfide bond in DraE is recognized by the DraB chaperone, indicating a mechanism that precludes the incorporation of less stable, non-oxidized DraE forms into the fimbriae. ..
  13. Dautant A, Meyer P, Georgescauld F. Hydrogen/Deuterium Exchange Mass Spectrometry Reveals Mechanistic Details of Activation of Nucleoside Diphosphate Kinases by Oligomerization. Biochemistry. 2017;56:2886-2896 pubmed publisher
    ..Further HDX-MS studies are necessary to establish the general activation mechanism for other homo-oligomers. ..
  14. Gao B, Harvey P, Craik D, Ronjat M, De Waard M, Zhu S. Functional evolution of scorpion venom peptides with an inhibitor cystine knot fold. Biosci Rep. 2013;33: pubmed publisher
    ..The structural and functional characterizations of the first scorpion venom ICK toxin with K+ channel-blocking activity sheds light on functionally divergent and convergent evolution of this conserved scaffold of ancient origin. ..
  15. Bond T, Sorenson A, Schaeffer P. Functional characterisation of Burkholderia pseudomallei biotin protein ligase: A toolkit for anti-melioidosis drug development. Microbiol Res. 2017;199:40-48 pubmed publisher
    ..Our biochemical data in combination with the new HT DSF-GTP and biotinylation activity assay could facilitate future drug screening efforts against this drug-resistant organism. ..
  16. Davies A, Allan E, Keeble A, Delgado J, Cossins B, Mitropoulou A, et al. Allosteric mechanism of action of the therapeutic anti-IgE antibody omalizumab. J Biol Chem. 2017;292:9975-9987 pubmed publisher
  17. Andhirka S, Vignesh R, Aradhyam G. The nucleotide-free state of heterotrimeric G proteins α-subunit adopts a highly stable conformation. FEBS J. 2017;284:2464-2481 pubmed publisher
  18. Kötzler M, McIntosh L, Withers S. Refolding the unfoldable: A systematic approach for renaturation of Bacillus circulans xylanase. Protein Sci. 2017;26:1555-1563 pubmed publisher
    ..This general approach should be applicable to related GH11 xylanases, as well as proteins adopting a similar ?-jellyroll fold, that are otherwise recalcitrant to refolding in vitro. ..