totipotent stem cells

Summary

Summary: Single cells that have the potential to form an entire organism. They have the capacity to specialize into extraembryonic membranes and tissues, the embryo, and all postembryonic tissues and organs. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)

Top Publications

  1. Macfarlan T, Gifford W, Driscoll S, Lettieri K, Rowe H, Bonanomi D, et al. Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature. 2012;487:57-63 pubmed publisher
  2. Wang X, Nolan K, Irwanto R, Sheahan M, Rose R. Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells. Ann Bot. 2011;107:599-609 pubmed publisher
    ..Here, the fate of leaf explant cells during the development of embryogenic callus was investigated in the model legume Medicago truncatula...
  3. Ilic D, Giritharan G, Zdravkovic T, Caceres E, Genbacev O, Fisher S, et al. Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials. Stem Cells Dev. 2009;18:1343-50 pubmed publisher
    ..All derived lines maintained normal karyotype and expressed totipotent phenotype including the ability to differentiate into trophectoderm and all three germ layers...
  4. Mitalipov S, Wolf D. Totipotency, pluripotency and nuclear reprogramming. Adv Biochem Eng Biotechnol. 2009;114:185-99 pubmed publisher
    ..In this review, we summarize two major approaches to reprogramming: (1) somatic cell nuclear transfer and (2) direct reprogramming using genetic manipulations...
  5. Morgani S, Canham M, Nichols J, Sharov A, Migueles R, Ko M, et al. Totipotent embryonic stem cells arise in ground-state culture conditions. Cell Rep. 2013;3:1945-57 pubmed publisher
    ..Thus, 2i and LIF support a totipotent state comparable to early embryonic cells that coexpress embryonic and extraembryonic determinants. ..
  6. Krichevsky A, Sonntag K, Isacson O, Kosik K. Specific microRNAs modulate embryonic stem cell-derived neurogenesis. Stem Cells. 2006;24:857-64 pubmed
    ..We conclude that distinct miRNAs play a functional role in the determination of neural fates in ES cell differentiation...
  7. Shigematsu Y, Yoshida N, Miwa Y, Mizobuti A, Suzuki Y, Tanimoto Y, et al. Novel embryonic stem cells expressing tdKaede protein photoconvertible from green to red fluorescence. Int J Mol Med. 2007;20:439-44 pubmed
    ..These novel ES cell lines ubiquitously expressing photoconvertible Kaede protein, B6KED-1 and -2, are useful for basic research in developmental biology and regenerative medicine...
  8. Müller W, Teo R, Frank U. Totipotent migratory stem cells in a hydroid. Dev Biol. 2004;275:215-24 pubmed
    ..With time, the recipient underwent a complete conversion into the phenotype and genotype of the donor. Thus, under these experimental conditions the interstitial stem cells of Hydractinia exhibit totipotency...
  9. Fulka H, St John J, Galli C, Lazzari G, Lagutina I, Fulka J, et al. Cybrid human embryos--warranting opportunities to augment embryonic stem cell research. Trends Biotechnol. 2008;26:469-74 pubmed publisher
    ..If this approach is to be successful, many biological questions need to be answered and, in addition, some moral and ethical aspects must be taken into account...
  10. Reis H. [Adult stem cells. Prospects for the therapy]. Med Klin (Munich). 2003;98 Suppl 2:2-3 pubmed

Detail Information

Publications62

  1. Macfarlan T, Gifford W, Driscoll S, Lettieri K, Rowe H, Bonanomi D, et al. Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature. 2012;487:57-63 pubmed publisher
  2. Wang X, Nolan K, Irwanto R, Sheahan M, Rose R. Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells. Ann Bot. 2011;107:599-609 pubmed publisher
    ..Here, the fate of leaf explant cells during the development of embryogenic callus was investigated in the model legume Medicago truncatula...
  3. Ilic D, Giritharan G, Zdravkovic T, Caceres E, Genbacev O, Fisher S, et al. Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials. Stem Cells Dev. 2009;18:1343-50 pubmed publisher
    ..All derived lines maintained normal karyotype and expressed totipotent phenotype including the ability to differentiate into trophectoderm and all three germ layers...
  4. Mitalipov S, Wolf D. Totipotency, pluripotency and nuclear reprogramming. Adv Biochem Eng Biotechnol. 2009;114:185-99 pubmed publisher
    ..In this review, we summarize two major approaches to reprogramming: (1) somatic cell nuclear transfer and (2) direct reprogramming using genetic manipulations...
  5. Morgani S, Canham M, Nichols J, Sharov A, Migueles R, Ko M, et al. Totipotent embryonic stem cells arise in ground-state culture conditions. Cell Rep. 2013;3:1945-57 pubmed publisher
    ..Thus, 2i and LIF support a totipotent state comparable to early embryonic cells that coexpress embryonic and extraembryonic determinants. ..
  6. Krichevsky A, Sonntag K, Isacson O, Kosik K. Specific microRNAs modulate embryonic stem cell-derived neurogenesis. Stem Cells. 2006;24:857-64 pubmed
    ..We conclude that distinct miRNAs play a functional role in the determination of neural fates in ES cell differentiation...
  7. Shigematsu Y, Yoshida N, Miwa Y, Mizobuti A, Suzuki Y, Tanimoto Y, et al. Novel embryonic stem cells expressing tdKaede protein photoconvertible from green to red fluorescence. Int J Mol Med. 2007;20:439-44 pubmed
    ..These novel ES cell lines ubiquitously expressing photoconvertible Kaede protein, B6KED-1 and -2, are useful for basic research in developmental biology and regenerative medicine...
  8. Müller W, Teo R, Frank U. Totipotent migratory stem cells in a hydroid. Dev Biol. 2004;275:215-24 pubmed
    ..With time, the recipient underwent a complete conversion into the phenotype and genotype of the donor. Thus, under these experimental conditions the interstitial stem cells of Hydractinia exhibit totipotency...
  9. Fulka H, St John J, Galli C, Lazzari G, Lagutina I, Fulka J, et al. Cybrid human embryos--warranting opportunities to augment embryonic stem cell research. Trends Biotechnol. 2008;26:469-74 pubmed publisher
    ..If this approach is to be successful, many biological questions need to be answered and, in addition, some moral and ethical aspects must be taken into account...
  10. Reis H. [Adult stem cells. Prospects for the therapy]. Med Klin (Munich). 2003;98 Suppl 2:2-3 pubmed
  11. Suwinska A, Czołowska R, Ożdżeński W, Tarkowski A. Blastomeres of the mouse embryo lose totipotency after the fifth cleavage division: expression of Cdx2 and Oct4 and developmental potential of inner and outer blastomeres of 16- and 32-cell embryos. Dev Biol. 2008;322:133-44 pubmed publisher
  12. Carlin R. Patents for human embryonic stem cells?. Med Device Technol. 2003;14:16 pubmed
  13. Hübner K, Fuhrmann G, Christenson L, Kehler J, Reinbold R, De La Fuente R, et al. Derivation of oocytes from mouse embryonic stem cells. Science. 2003;300:1251-6 pubmed
    ..Oogenesis in culture should contribute to various areas, including nuclear transfer and manipulation of the germ line, and advance studies on fertility treatment and germ and somatic cell interaction and differentiation...
  14. Dean W, Santos F, Reik W. Epigenetic reprogramming in early mammalian development and following somatic nuclear transfer. Semin Cell Dev Biol. 2003;14:93-100 pubmed
    ..We propose the concept of 'epigenetic checkpoints' for normal progression of development and the loss of totipotency...
  15. Surani A, Tischler J. Stem cells: a sporadic super state. Nature. 2012;487:43-5 pubmed publisher
  16. Solana J, Lasko P, Romero R. Spoltud-1 is a chromatoid body component required for planarian long-term stem cell self-renewal. Dev Biol. 2009;328:410-21 pubmed publisher
    Freshwater planarians exhibit a striking power of regeneration, based on a population of undifferentiated totipotent stem cells, called neoblasts...
  17. Klopsch C, Donndorf P, Kaminski A, Ma N, Steinhoff G. [Cell sources for cardiovascular tissue engineering]. Chirurg. 2011;82:295-302 pubmed publisher
  18. Shah S, Lowery E, Braun R, Martin A, Huang N, Medina M, et al. Cellular basis of tissue regeneration by omentum. PLoS ONE. 2012;7:e38368 pubmed publisher
    ..Based on these data, we propose that the omentum is a designated organ for tissue repair and healing in response to foreign invasion and tissue damage...
  19. Denker H. Totipotency/pluripotency and patentability. Stem Cells. 2008;26:1656-7 pubmed publisher
    ..In their article entitled "..
  20. Torres Padilla M. Cell identity in the preimplantation mammalian embryo: an epigenetic perspective from the mouse. Hum Reprod. 2008;23:1246-52 pubmed publisher
  21. Ratajczak M, Zuba Surma E, Wysoczynski M, Wan W, Ratajczak J, Wojakowski W, et al. Hunt for pluripotent stem cell -- regenerative medicine search for almighty cell. J Autoimmun. 2008;30:151-62 pubmed publisher
    ..This review will summarize current approaches and limitations to isolate PSC from adult tissues or, alternatively, to generate it by nuclear reprogramming from already differentiated somatic cells...
  22. Lesch B, Dokshin G, Young R, McCarrey J, Page D. A set of genes critical to development is epigenetically poised in mouse germ cells from fetal stages through completion of meiosis. Proc Natl Acad Sci U S A. 2013;110:16061-6 pubmed publisher
    ..We propose that the epigenetically poised condition of these developmental genes is a fundamental property of the mammalian germ-line nucleus, allowing differentiated gametes to unleash a totipotent program following fertilization. ..
  23. Seydoux G, Braun R. Pathway to totipotency: lessons from germ cells. Cell. 2006;127:891-904 pubmed
    ..Transcriptional repression, chromatin remodeling, and an emphasis on posttranscriptional gene regulation preserve the totipotent genome of germ cells through generations...
  24. Meissner A, Jaenisch R. Mammalian nuclear transfer. Dev Dyn. 2006;235:2460-9 pubmed
    ..In the long term, nuclear transfer or alternative strategies aim to generate customized pluripotent cells, which would be invaluable to medical research and therapy...
  25. Li S, Yurchenco P. Matrix assembly, cell polarization, and cell survival: analysis of peri-implantation development with cultured embryonic stem cells. Methods Mol Biol. 2006;329:113-25 pubmed
  26. Lochner J. The ethics of research on stem cells of human origin. S Afr Med J. 2002;92:779-82 pubmed
  27. Ezhova T. [Genetic control of totipotency of plant cells in vitro]. Ontogenez. 2003;34:245-52 pubmed
  28. Hug K. Sources of human embryos for stem cell research: ethical problems and their possible solutions. Medicina (Kaunas). 2005;41:1002-10 pubmed
    ..The aim of this literature review is to present the main arguments for and against using different sources of human embryonic stem cells and to acquaint with possible alternatives to human embryo research...
  29. Metcalf D. The unsolved enigmas of leukemia inhibitory factor. Stem Cells. 2003;21:5-14 pubmed
  30. Anversa P, Kajstura J, Leri A. Circulating progenitor cells: search for an identity. Circulation. 2004;110:3158-60 pubmed
  31. Isaeva V, Shukaliuk A, Kizilova E. [Recognition of stem cells in the colonial internae of the Rhizocephala Peltogasterella gracilis and Sacculina polygenea at the parasitic life cycle stage]. Tsitologiia. 2003;45:758-63 pubmed
    ..The cytochemical manifestation of alkaline phosphatase activity in totipotent stem cells of the colonial rhizocephala is the first evidence of commonality in the functional characteristics of ..
  32. Sinha S, Hoofnagle M, Kingston P, McCanna M, Owens G. Transforming growth factor-beta1 signaling contributes to development of smooth muscle cells from embryonic stem cells. Am J Physiol Cell Physiol. 2004;287:C1560-8 pubmed
    ..These results are the first to provide direct evidence that TGF-beta1 signaling through Smad2 and Smad3 plays an important role in the development of SMCs from totipotential ESCs...
  33. Behr B, Ko S, Wong V, Gurtner G, Longaker M. Stem cells. Plast Reconstr Surg. 2010;126:1163-71 pubmed publisher
  34. Juliano C, Wessel G. Developmental biology. Versatile germline genes. Science. 2010;329:640-1 pubmed publisher
  35. Kato Y, Imabayashi H, Mori T, Tani T, Taniguchi M, Higashi M, et al. Nuclear transfer of adult bone marrow mesenchymal stem cells: developmental totipotency of tissue-specific stem cells from an adult mammal. Biol Reprod. 2004;70:415-8 pubmed
    ..Here, we showed the developmental totipotency of adult bovine mesenchymal stem cells by nuclear transfer...
  36. Psarras S, Karagianni N, Kellendonk C, Tronche F, Cosset F, Stocking C, et al. Gene transfer and genetic modification of embryonic stem cells by Cre- and Cre-PR-expressing MESV-based retroviral vectors. J Gene Med. 2004;6:32-42 pubmed
    ..PR) fusion in mouse fibroblasts and ES cells...
  37. Morey C, Navarro P, Debrand E, Avner P, Rougeulle C, Clerc P. The region 3' to Xist mediates X chromosome counting and H3 Lys-4 dimethylation within the Xist gene. EMBO J. 2004;23:594-604 pubmed
    ..Our results raise the possibility that H3 Lys-4 dimethylation within Xist may be functionally implicated in the counting process...
  38. Roelen B, Lopes S. Of stem cells and gametes: similarities and differences. Curr Med Chem. 2008;15:1249-56 pubmed
  39. BIRNBAUM K, SANCHEZ ALVARADO A. Slicing across kingdoms: regeneration in plants and animals. Cell. 2008;132:697-710 pubmed publisher
  40. Oh S, Kim H, Ahn H, Seol H, Kim Y, Park Y, et al. Derivation and characterization of new human embryonic stem cell lines: SNUhES1, SNUhES2, and SNUhES3. Stem Cells. 2005;23:211-9 pubmed
    ..When in vitro differentiation potentials of these cell lines into cardiomyocytes were examined, SNUhES3 was found to differentiate into cardiomyocytes most effectively...
  41. Stout C, Ashley D, Morgan J, Long G, Collins J, Limnios J, et al. Primitive stem cells residing in the skeletal muscle of adult pigs are mobilized into the peripheral blood after trauma. Am Surg. 2007;73:1106-10 pubmed
    ..Further studies are required to elucidate the roles that adult-derived BLSCs play in the response to injury and in the healing process. Surgeons must take a role in this evolving field...
  42. de Kretser D. Totipotent, pluripotent or unipotent stem cells: a complex regulatory enigma and fascinating biology. J Law Med. 2007;15:212-8 pubmed
  43. Bryja V, Bonilla S, Cajanek L, Parish C, Schwartz C, Luo Y, et al. An efficient method for the derivation of mouse embryonic stem cells. Stem Cells. 2006;24:844-9 pubmed
    ..In summary, we provide a simplified and improved protocol to derive mESCs that can be useful for laboratories aiming to isolate transgenic mESCs for the first time...
  44. Chupeau M, Granier F, Pichon O, Renou J, Gaudin V, Chupeau Y. Characterization of the early events leading to totipotency in an Arabidopsis protoplast liquid culture by temporal transcript profiling. Plant Cell. 2013;25:2444-63 pubmed publisher
    ..One of these genes, the plant-specific transcription factor ABERRANT LATERAL ROOT FORMATION4, is required for the initiation of protoplast division...
  45. Zuk P. The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used. J Cell Biochem. 2009;106:867-77 pubmed publisher
    ..Therefore, confirming the expression of OCT4, Sox2, and Nanog in adult stem cells with today's commercial antibodies must be carefully considered before the designation of pluripotent can be granted...
  46. Pan G, Chang Z, Scholer H, Pei D. Stem cell pluripotency and transcription factor Oct4. Cell Res. 2002;12:321-9 pubmed
    ..In this review, we summarize the structure and function of Oct4 and address issues related to Oct4 function in maintaining totipotency or pluripotency of embryonic stem cells...
  47. Tursun B, Patel T, Kratsios P, Hobert O. Direct conversion of C. elegans germ cells into specific neuron types. Science. 2011;331:304-8 pubmed publisher
    ..Our findings illustrate the ability of germ cells to be directly converted into individual, terminally differentiated neuron types and demonstrate that a specific chromatin factor provides a barrier for cellular reprogramming...
  48. Tanaka T, Kunath T, Kimber W, Jaradat S, Stagg C, Usuda M, et al. Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity. Genome Res. 2002;12:1921-8 pubmed
    ..We suggest that pluripotency requires a set of genes not expressed in other cell types, whereas lineage-restricted stem cells, like TS cells, express genes predictive of their differentiated lineage...
  49. Surani M, Hajkova P. Epigenetic reprogramming of mouse germ cells toward totipotency. Cold Spring Harb Symp Quant Biol. 2010;75:211-8 pubmed publisher
    ..Detection of Tet1 expression specifically and coincidentally, at the time of BER in PGCs, suggests that conversion of 5mC to 5hmC might be involved, at least in part, during epigenetic reprogramming and DNA demethylation in germ cells...
  50. Ivanova N, Dimos J, Schaniel C, Hackney J, Moore K, Lemischka I. A stem cell molecular signature. Science. 2002;298:601-4 pubmed
    ..Moreover, in the mouse, a portion of the genetic program of hematopoietic stem cells is shared with embryonic and neural stem cells. This overlapping set of gene products represents a molecular signature of stem cells...
  51. Brehm K. Echinococcus multilocularis as an experimental model in stem cell research and molecular host-parasite interaction. Parasitology. 2010;137:537-55 pubmed publisher
    ..multilocularis sequencing project, stem cell cultivation, and recently initiated attempts to genetically manipulate Echinococcus will provide an ideal platform for hypothesis-driven research into cestode development in the next years...
  52. Sun Y, Kong W, Falk A, Hu J, Zhou L, Pollard S, et al. CD133 (Prominin) negative human neural stem cells are clonogenic and tripotent. PLoS ONE. 2009;4:e5498 pubmed publisher
    ..However, the assumption that CD133 is expressed constitutively in neural precursor cells has not been examined...
  53. Sato H, Amagai K, Shimizukawa R, Tamai Y. Stable generation of serum- and feeder-free embryonic stem cell-derived mice with full germline-competency by using a GSK3 specific inhibitor. Genesis. 2009;47:414-22 pubmed publisher
    ..We hereby propose that serum- and feeder-free B6 ES cells stimulated with LIF plus GSK3 inhibitor are valuable for generating mouse models on B6 background...
  54. Lysdahl H, Gabrielsen A, Minger S, Patel M, Fink T, Petersen K, et al. Derivation and characterization of four new human embryonic stem cell lines: the Danish experience. Reprod Biomed Online. 2006;12:119-26 pubmed
    ..Finally, the cells displayed the potential to differentiate in vitro into cell types originating from all three germ layers. It is thought that the cell lines described in this study are the first human ES cells established in Denmark...
  55. Ikuta L. Human umbilical cord blood transplantation: what nurses need to know. AACN Adv Crit Care. 2008;19:264-7 pubmed publisher
  56. Zeng X, Rao M. The therapeutic potential of embryonic stem cells: A focus on stem cell stability. Curr Opin Mol Ther. 2006;8:338-44 pubmed
    ..Recent findings suggesting that the level of cell stability is not homogeneous throughout all stem cells are also discussed...
  57. Gallo C, Seydoux G. Toti "potent" repressors. Bioessays. 2006;28:865-7 pubmed
    ..The unexpected discovery of a teratoma in a C. elegans double mutant points to translational control as a key mechanism to maintain totipotency in developing germ cells...
  58. Corley L, Lavine M. A review of insect stem cell types. Semin Cell Dev Biol. 2006;17:510-7 pubmed
    ..In this review we discuss the current state of knowledge of insect stem cell types. We examine what stem cell types have been identified from insects, and briefly discuss what is known about their regulation...
  59. Guo J, Jauch A, Heidi H, Schoell B, Erz D, Schrank M, et al. Multicolor karyotype analyses of mouse embryonic stem cells. In Vitro Cell Dev Biol Anim. 2005;41:278-83 pubmed
    ..We demonstrate that M-FISH is a sensitive and accurate method for a comprehensive karyotype analysis of ES cells and may minimize time, costs, and disappointments due to inadequate ES cell sources...
  60. Daley G. ES cells prove egg-straordinary. Nat Biotechnol. 2003;21:760-1 pubmed
  61. Karbanova J, Mokry J. Histological and histochemical analysis of embryoid bodies. Acta Histochem. 2002;104:361-5 pubmed
    ..Our results demonstrate the usefulness of this in vitro model for studying early embryogenesis...
  62. Kobayashi K, Hashiguchi T, Ichikawa T, Ishino Y, Hoshi M, Matsumoto M. Neoblast-enriched fraction rescues eye formation in eye-defective planarian 'menashi' Dugesia ryukyuensis. Dev Growth Differ. 2008;50:689-96 pubmed publisher
    ..These cells can replace the cells and structures lost by X-ray irradiation and ablation, and they can also differentiate into eye pigment cells...