human embryonic stem cells


Summary: A type of PLURIPOTENT STEM CELLS derived from early stage human embryos, up to and including the BLASTOCYST stage.

Top Publications

  1. Chakradhar S. An eye to the future: Researchers debate best path for stem cell-derived therapies. Nat Med. 2016;22:116-9 pubmed publisher
  2. Takayama K, Igai K, Hagihara Y, Hashimoto R, Hanawa M, Sakuma T, et al. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system. Nucleic Acids Res. 2017;45:5198-5207 pubmed publisher
    ..Importantly, RAD51 overexpression and valproic acid treatment synergistically increased the biallelic homologous recombination efficiency. Our findings would facilitate genome editing study using human ES/iPS cells. ..
  3. Waraky A, Lin Y, Warsito D, Haglund F, Aleem E, Larsson O. Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage. J Biol Chem. 2017;292:18227-18239 pubmed publisher
    ..We found that IGF-1R interacts with and phosphorylates PCNA in human embryonic stem cells and other cell lines...
  4. Kim S, Habib O, Kim J, Han H, Koo S, Kim J. A homozygous Keap1-knockout human embryonic stem cell line generated using CRISPR/Cas9 mediates gene targeting. Stem Cell Res. 2017;19:52-54 pubmed publisher
    ..The Keap1 homozygous-knockout H9 cell line retained normal morphology, gene expression, and in vivo differentiation potential. ..
  5. Ferguson L, Balaiya S, Mynampati B, Sambhav K, Chalam K. Deprivation of bFGF Promotes Spontaneous Differentiation of Human Embryonic Stem Cells into Retinal Pigment Epithelial Cells. J Stem Cells. 2015;10:159-70 pubmed
    ..b>Human embryonic stem cells derived RPE (hESC-RPE) is ideal for cell based therapy because of their ability to morphologically and ..
  6. Chan P, Geng L, Gao Y, Keung W, Li R. AAV-mediated conversion of human pluripotent stem cell-derived pacemaker. Biochem Biophys Res Commun. 2017;494:346-351 pubmed publisher
    ..Collectively, these results have further laid the groundwork on BPm for future translation...
  7. Hübner K, Grassme K, Rao J, Wenke N, Zimmer C, Korte L, et al. Wnt signaling positively regulates endothelial cell fate specification in the Fli1a-positive progenitor population via Lef1. Dev Biol. 2017;430:142-155 pubmed publisher
    ..Using mesoderm derived from human embryonic stem cells, we identified the same principle of Wnt signaling dependent EC specification in conjunction with auto-..
  8. Begum A, Aguilar J, Hong Y. Aqueous cigarette tar extracts disrupt corticogenesis from human embryonic stem cells in vitro. Environ Res. 2017;158:194-202 pubmed publisher
    ..and then applied at different concentrations to neuronal progenitors and cortical neurons derived from human embryonic stem cells. ACTEs reduced the expression of the cortical neuronal progenitor markers pax6, tbr2, and neuroD and ..
  9. Crespo M, Vilar E, Tsai S, Chang K, Amin S, Srinivasan T, et al. Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing. Nat Med. 2017;23:878-884 pubmed publisher
    ..we sought to develop an effective protocol for deriving colonic organoids (COs) from differentiated human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs)...

More Information


  1. Ascensión A, Arrospide Elgarresta M, Izeta A, Arauzo Bravo M. NaviSE: superenhancer navigator integrating epigenomics signal algebra. BMC Bioinformatics. 2017;18:296 pubmed publisher
    ..NaviSE binaries and documentation are available at: . ..
  2. Merkle F, Ghosh S, Kamitaki N, Mitchell J, Avior Y, Mello C, et al. Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations. Nature. 2017;545:229-233 pubmed publisher
  3. Ounpuu L, Klepinin A, Pook M, Teino I, Peet N, Paju K, et al. 2102Ep embryonal carcinoma cells have compromised respiration and shifted bioenergetic profile distinct from H9 human embryonic stem cells. Biochim Biophys Acta Gen Subj. 2017;1861:2146-2154 pubmed publisher
    ..The aim of this study was to compare the mitochondrial bioenergetic profile of normal pluripotent human embryonic stem cells (hESC) and relatively nullipotent embryonal carcinoma cells (2102Ep cell line)...
  4. Zhang C, Du L, Pang K, Wu X. Differentiation of human embryonic stem cells into corneal epithelial progenitor cells under defined conditions. PLoS ONE. 2017;12:e0183303 pubmed publisher
    ..The aim of this study was to develop a novel protocol to differentiate human embryonic stem cells (hESCs) into corneal epithelial progenitor cells (CEPCs), with similar features to primary cultured ..
  5. Suchorska W, Augustyniak E, Richter M, Łukjanow M, Filas V, Kaczmarczyk J, et al. Modified methods for efficiently differentiating human embryonic stem cells into chondrocyte-like cells. Postepy Hig Med Dosw (Online). 2017;71:500-509 pubmed
    ..So far, numerous cell types have been reported to show chondrogenic potential, among others human embryonic stem cells (hESCs)...
  6. Liu Q, Jiang C, Xu J, Zhao M, Van Bortle K, Cheng X, et al. Genome-Wide Temporal Profiling of Transcriptome and Open Chromatin of Early Cardiomyocyte Differentiation Derived From hiPSCs and hESCs. Circ Res. 2017;121:376-391 pubmed publisher
    ..improved our ability to generate cardiomyocytes from human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs)...
  7. Deng Y, Bao F, Yang Y, Ji X, Du M, Zhang Z, et al. Information transduction capacity reduces the uncertainties in annotation-free isoform discovery and quantification. Nucleic Acids Res. 2017;45:e143 pubmed publisher
    ..Our algorithm is an open-source implementation of MaxInfo. ..
  8. Liu Z, Lou H, Xie K, Wang H, Chen N, Aparicio O, et al. Reconstructing cell cycle pseudo time-series via single-cell transcriptome data. Nat Commun. 2017;8:22 pubmed publisher
  9. Kim S, Habib O, Kim J, Han H, Koo S, Kim J. Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9. Stem Cell Res. 2017;19:46-48 pubmed publisher
    ..The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype. ..
  10. Matsushita M, Nakatake Y, Arai I, Ibata K, Kohda K, Goparaju S, et al. Neural differentiation of human embryonic stem cells induced by the transgene-mediated overexpression of single transcription factors. Biochem Biophys Res Commun. 2017;490:296-301 pubmed publisher
    Pluripotent human embryonic stem cells (hESCs) can differentiate into multiple cell lineages, thus, providing one of the best platforms to study molecular mechanisms during cell differentiation...
  11. Bektik E, Dennis A, Prasanna P, Madabhushi A, Fu J. Single cell qPCR reveals that additional HAND2 and microRNA-1 facilitate the early reprogramming progress of seven-factor-induced human myocytes. PLoS ONE. 2017;12:e0183000 pubmed publisher
    ..Our study provides valuable information to further optimize a method of direct iCM-reprogramming in human cells. ..
  12. Atlasi Y, Stunnenberg H. The interplay of epigenetic marks during stem cell differentiation and development. Nat Rev Genet. 2017;18:643-658 pubmed publisher
  13. Sun H, Wang X, Liu K, Guo M, Zhang Y, Ying Q, et al. ?-catenin coordinates with Jup and the TCF1/GATA6 axis to regulate human embryonic stem cell fate. Dev Biol. 2017;431:272-281 pubmed publisher
    catenin-mediated signaling has been extensively studied in regard to its role in the regulation of human embryonic stem cells (hESCs)...
  14. Wu S, Li K, Li Y, Zhao T, Li T, Yang Y, et al. Independent regulation of gene expression level and noise by histone modifications. PLoS Comput Biol. 2017;13:e1005585 pubmed publisher
  15. Mirsadeghi S, Shahbazi E, Hemmesi K, Nemati S, Baharvand H, Mirnajafi Zadeh J, et al. Development of membrane ion channels during neural differentiation from human embryonic stem cells. Biochem Biophys Res Commun. 2017;491:166-172 pubmed publisher
    For human embryonic stem cells (hESCs) to differentiate into neurons, enormous changes has to occur leading to trigger action potential and neurotransmitter release...
  16. Nemashkalo A, Ruzo A, Heemskerk I, Warmflash A. Morphogen and community effects determine cell fates in response to BMP4 signaling in human embryonic stem cells. Development. 2017;144:3042-3053 pubmed publisher
    ..states and create cell fate patterns in vivo and influence differentiation outcomes in human embryonic stem cells (hESCs) in vitro Systematic investigation of morphogen signaling is hampered by the difficulty of ..
  17. Fogarty N, McCarthy A, Snijders K, Powell B, Kubikova N, Blakeley P, et al. Genome editing reveals a role for OCT4 in human embryogenesis. Nature. 2017;550:67-73 pubmed publisher
    ..We conclude that CRISPR-Cas9-mediated genome editing is a powerful method for investigating gene function in the context of human development. ..
  18. Poliandri A, Miller D, Howard S, Nobles M, Ruiz Babot G, Harmer S, et al. Generation of kisspeptin-responsive GnRH neurons from human pluripotent stem cells. Mol Cell Endocrinol. 2017;447:12-22 pubmed publisher
    ..the efficient generation of kisspeptin responsive GnRH-secreting neurons by directed differentiation of human Embryonic Stem Cells and induced-Pluripotent Stem Cells derived from a Kallman Syndrome patient and a healthy family member...
  19. Neubauer J, Stracke F, Zimmermann H. Chapter 17 Sterile Plate-Based Vitrification of Adherent Human Pluripotent Stem Cells and Their Derivatives Using the TWIST Method. Methods Mol Biol. 2017;1568:231-241 pubmed publisher
    ..adherent pluripotent stem cells and their derivatives is presented based on a procedure and device for human embryonic stem cells developed by Beier et al. (Cryobiology 66:8-16, 2013)...
  20. Korsgren O. Islet Encapsulation: Physiological Possibilities and Limitations. Diabetes. 2017;66:1748-1754 pubmed publisher
    ..Generation of transplantable insulin-producing cells from human embryonic stem cells or induced pluripotent stem cells is at present close to reality, and we are currently awaiting the ..
  21. Kurapati S, Sadaoka T, Rajbhandari L, Jagdish B, Shukla P, Ali M, et al. Role of the JNK Pathway in Varicella-Zoster Virus Lytic Infection and Reactivation. J Virol. 2017;91: pubmed publisher
    ..The identification of the role of the JNK pathway in VZV infection of neurons reveals potential avenues for the development of alternate antiviral drugs. ..
  22. Araújo M, Kyrylenko S, Spejo A, Castro M, Ferreira Junior R, Barraviera B, et al. Transgenic human embryonic stem cells overexpressing FGF2 stimulate neuroprotection following spinal cord ventral root avulsion. Exp Neurol. 2017;294:45-57 pubmed publisher
    ..Thus, we investigated neuroprotection elicited by human embryonic stem cells (hESC), modified to overexpress a human fibroblast growth factor 2 (FGF-2), on motoneurons subjected to ..
  23. Mao G, Lu P, Wang Y, Tian C, Huang X, Feng Z, et al. Role of PI3K p110? in the differentiation of human embryonic stem cells into islet-like cells. Biochem Biophys Res Commun. 2017;488:109-115 pubmed publisher
    ..the effects of the PI3K inhibitors on the differentiation of insulin-producing cells derived from human embryonic stem cells. Here, we report that human embryonic stem cells induced by phosphatidylinositol-3-kinase (PI3K) p110? ..
  24. Skottman H, Muranen J, Lähdekorpi H, Pajula E, Makela K, Koivusalo L, et al. Contacting co-culture of human retinal microvascular endothelial cells alters barrier function of human embryonic stem cell derived retinal pigment epithelial cells. Exp Cell Res. 2017;359:101-111 pubmed publisher
    ..In addition, co-culture revealed drastic cell line specific differences, most notably in growth factor secretion. This model has the potential to be used as an in vitro outer blood-retinal barrier model for drug permeability testing...
  25. Welsbie D, Mitchell K, Jaskula Ranga V, Sluch V, Yang Z, Kim J, et al. Enhanced Functional Genomic Screening Identifies Novel Mediators of Dual Leucine Zipper Kinase-Dependent Injury Signaling in Neurons. Neuron. 2017;94:1142-1154.e6 pubmed publisher
    ..Increased understanding of the DLK pathway has implications for understanding and treating neurodegenerative diseases. ..