protoplasts

Summary

Summary: The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.

Top Publications

  1. Coelho S, Scornet D, Rousvoal S, Peters N, Dartevelle L, Peters A, et al. Isolation and regeneration of protoplasts from Ectocarpus. Cold Spring Harb Protoc. 2012;2012:361-4 pubmed publisher
    ..The resultant protoplasts are totipotent and regenerate to produce individual algae under appropriate culture conditions...
  2. Guo X, Xie C, Cai X, Song B, He L, Liu J. Meiotic behavior of pollen mother cells in relation to ploidy level of somatic hybrids between Solanum tuberosum and S. chacoense. Plant Cell Rep. 2010;29:1277-85 pubmed publisher
    ..The hexaploids with stable genome dosage and a certain level of fertility will have great potential in a potato breeding program...
  3. Wang Y, Gehring C, Irving H. Plant natriuretic peptides are apoplastic and paracrine stress response molecules. Plant Cell Physiol. 2011;52:837-50 pubmed publisher
  4. Ieronimo M, Afonin S, Koch K, Berditsch M, Wadhwani P, Ulrich A. 19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes from bacterial protoplasts and human erythrocytes. J Am Chem Soc. 2010;132:8822-4 pubmed publisher
    ..For solid-state NMR analysis, we isolated native membranes from erythrocyte ghosts and bacterial protoplasts and prepared them as macroscopically oriented samples...
  5. Wang J, Ding H, Zhang A, Ma F, Cao J, Jiang M. A novel mitogen-activated protein kinase gene in maize (Zea mays), ZmMPK3, is involved in response to diverse environmental cues. J Integr Plant Biol. 2010;52:442-52 pubmed publisher
    ..Furthermore, the results showed that the ZmMPK3 protein is localized mainly to the nucleus. These results suggest that the ZmMPK3 may play an important role in response to environmental stresses...
  6. Tewari R, Watanabe D, Watanabe M. Chloroplastic NADPH oxidase-like activity-mediated perpetual hydrogen peroxide generation in the chloroplast induces apoptotic-like death of Brassica napus leaf protoplasts. Planta. 2012;235:99-110 pubmed publisher
    Despite extensive research over the past years, regeneration from protoplasts has been observed in only a limited number of plant species. Protoplasts undergo complex metabolic modification during their isolation...
  7. Ul Rehman R, Rinalducci S, Zolla L, Dalessandro G, Di Sansebastiano G. Nicotiana tabacum protoplasts secretome can evidence relations among regulatory elements of exocytosis mechanisms. Plant Signal Behav. 2011;6:1140-5 pubmed publisher
    ..b>Protoplasts present many convenient features to study cellular processes during transient over-expression or suppression of ..
  8. Farage Barhom S, Burd S, Sonego L, Mett A, Belausov E, Gidoni D, et al. Localization of the Arabidopsis senescence- and cell death-associated BFN1 nuclease: from the ER to fragmented nuclei. Mol Plant. 2011;4:1062-73 pubmed publisher
    ..Analysis of BFN1-GFP localization in transiently transformed tobacco protoplasts revealed initial localization in filamentous structures spread throughout the cytoplasm, which then clustered ..
  9. Iwata Y, Lee M, Koizumi N. Analysis of a transcription factor using transient assay in Arabidopsis protoplasts. Methods Mol Biol. 2011;754:107-17 pubmed publisher
    ..Here we present a protocol for polyethylene glycol (PEG)-mediated transformation of Arabidopsis protoplasts. It details preparation of protoplasts from Arabidopsis suspension cultured cells or leaves of soil-grown ..

Scientific Experts

More Information

Publications62

  1. Zhang F, Maeder M, Unger Wallace E, Hoshaw J, Reyon D, Christian M, et al. High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc Natl Acad Sci U S A. 2010;107:12028-33 pubmed publisher
    ..Taken together, our results suggest that it should now be possible to obtain mutations in any Arabidopsis target gene regardless of its mutant phenotype...
  2. Silva A, Nguyen A, Ye C, Verchot J, Moon J. Conjugated polymer nanoparticles for effective siRNA delivery to tobacco BY-2 protoplasts. BMC Plant Biol. 2010;10:291 pubmed publisher
    ..Plant biologists have employed electroporation to deliver artificial siRNAs to plant protoplasts to study gene expression mechanisms at the single cell level...
  3. Yadav M, Bhatla S. Localization of lipoxygenase activity on the oil bodies and in protoplasts using a novel fluorescence imaging method. Plant Physiol Biochem. 2011;49:230-4 pubmed publisher
    ..This work provides new information on spatial localization of transiently enhanced LOX activity in protoplasts from 5 d old seedling cotyledons of sunflower (Helianthus annuus L. cv...
  4. Fettke J, Malinova I, Albrecht T, Hejazi M, Steup M. Glucose-1-phosphate transport into protoplasts and chloroplasts from leaves of Arabidopsis. Plant Physiol. 2011;155:1723-34 pubmed publisher
    ..two Glc-1-P-related processes: the carbon flux from externally supplied Glc-1-P to starch by either mesophyll protoplasts or intact chloroplasts from Arabidopsis (Arabidopsis thaliana)...
  5. Lord C, Gunawardena A. Environmentally induced programmed cell death in leaf protoplasts of Aponogeton madagascariensis. Planta. 2011;233:407-21 pubmed publisher
    ..heat shock (HS) treatment at 55 °C for 20 min as a promising inducer of environmental PCD within lace plant protoplasts originally isolated from non-PCD areas of the plant...
  6. Keerberg O, Ivanova H, Keerberg H, Pärnik T, Talts P, Gardeström P. Quantitative analysis of photosynthetic carbon metabolism in protoplasts and intact leaves of barley. Determination of carbon fluxes and pool sizes of metabolites in different cellular compartments. Biosystems. 2011;103:291-301 pubmed publisher
    Rates of carbon fluxes and pool sizes of photosynthetic metabolites in different cellular compartments of barley protoplasts were calculated from the time curves of their labeling in the medium of ¹?CO?...
  7. Yemelyanov V, Shishova M, Chirkova T, Lindberg S. Anoxia-induced elevation of cytosolic Ca2+ concentration depends on different Ca2+ sources in rice and wheat protoplasts. Planta. 2011;234:271-80 pubmed publisher
    ..The [Ca(2+)](cyt) was measured by fluorescence microscopy in single protoplasts loaded with the calcium-fluoroprobe Fura 2-AM...
  8. Munemasa S, Hossain M, Nakamura Y, Mori I, Murata Y. The Arabidopsis calcium-dependent protein kinase, CPK6, functions as a positive regulator of methyl jasmonate signaling in guard cells. Plant Physiol. 2011;155:553-61 pubmed publisher
    ..These results provide genetic evidence that CPK6 has a different role from CPK3 and functions as a positive regulator of MeJA signaling in Arabidopsis guard cells...
  9. Kanematsu S, Sasaki A, Onoue M, Oikawa Y, Ito T. Extending the fungal host range of a partitivirus and a mycoreovirus from Rosellinia necatrix by inoculation of protoplasts with virus particles. Phytopathology. 2010;100:922-30 pubmed publisher
    ..Here, we extended the experimental host range of these two mycoviruses using a transfection approach. Protoplasts of other phytopathogenic Sordariomycetous fungi-Diaporthe sp...
  10. Thieme R, Rakosy Tican E, Nachtigall M, Schubert J, Hammann T, Antonova O, et al. Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars. Plant Cell Rep. 2010;29:1187-201 pubmed publisher
  11. Kim J, Somers D. Rapid assessment of gene function in the circadian clock using artificial microRNA in Arabidopsis mesophyll protoplasts. Plant Physiol. 2010;154:611-21 pubmed publisher
    ..using transient expression of artificial microRNAs (amiRNAs) in Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts. We use a luciferase-based reporter of circadian clock activity to optimize and validate this system...
  12. Wehner N, Hartmann L, Ehlert A, Böttner S, Oñate Sánchez L, Dröge Laser W. High-throughput protoplast transactivation (PTA) system for the analysis of Arabidopsis transcription factor function. Plant J. 2011;68:560-9 pubmed publisher
    ..This novel molecular tool will improve functional studies on transcriptional regulation in plants significantly...
  13. Li J, Bush J, Xiong Y, Li L, McCormack M. Large-scale protein-protein interaction analysis in Arabidopsis mesophyll protoplasts by split firefly luciferase complementation. PLoS ONE. 2011;6:e27364 pubmed publisher
    ..Our results demonstrated that the SFLC assay is ideal for in vivo quantitative PPI analysis in plant cells and is particularly powerful for large-scale binary PPI screens...
  14. Isner J, Nühse T, Maathuis F. The cyclic nucleotide cGMP is involved in plant hormone signalling and alters phosphorylation of Arabidopsis thaliana root proteins. J Exp Bot. 2012;63:3199-205 pubmed publisher
    ..Out of these, nine were previously shown to also alter phosphorylation status in response to plant hormones, pointing to protein phosphorylation as a target for hormone-induced cGMP signalling...
  15. Shi B, Ni L, Zhang A, Cao J, Zhang H, Qin T, et al. OsDMI3 is a novel component of abscisic acid signaling in the induction of antioxidant defense in leaves of rice. Mol Plant. 2012;5:1359-74 pubmed publisher
    ..The analysis of the transient expression of OsDMI3 in rice protoplasts and the RNA interference (RNAi) silencing of OsDMI3 in rice protoplasts showed that OsDMI3 is required for ABA-..
  16. Jiang F, Zhu J, Liu H. Protoplasts: a useful research system for plant cell biology, especially dedifferentiation. Protoplasma. 2013;250:1231-8 pubmed publisher
    As protoplasts have the characteristics of no cell walls, rapid population growth, and synchronicity, they are useful tools for research in many fields, especially cellular biology (Table 1)...
  17. Isner J, Maathuis F. Measurement of cellular cGMP in plant cells and tissues using the endogenous fluorescent reporter FlincG. Plant J. 2011;65:329-34 pubmed publisher
  18. Langhans M, Förster S, Helmchen G, Robinson D. Differential effects of the brefeldin A analogue (6R)-hydroxy-BFA in tobacco and Arabidopsis. J Exp Bot. 2011;62:2949-57 pubmed publisher
    ..By contrast, while the 6(R)-hydroxy-BFA analogue gave rise to a classic BFA response in tobacco mesophyll protoplasts and true leaves of Arabidopsis (redistribution of Golgi enzymes into the ER), it failed to cause the formation ..
  19. Galbraith D, Janda J, Lambert G. Multiparametric analysis, sorting, and transcriptional profiling of plant protoplasts and nuclei according to cell type. Methods Mol Biol. 2011;699:407-29 pubmed publisher
    ..These illustrate the use of flow cytometry for the analysis of protoplasts and nuclear DNA contents (genome size and the cell cycle)...
  20. Masani M, Noll G, Parveez G, Sambanthamurthi R, Prüfer D. Regeneration of viable oil palm plants from protoplasts by optimizing media components, growth regulators and cultivation procedures. Plant Sci. 2013;210:118-27 pubmed publisher
    Oil palm protoplasts are suitable as a starting material for the production of oil palm plants with new traits using approaches such as somatic hybridization, but attempts to regenerate viable plants from protoplasts have failed thus far...
  21. Kwezi L, Ruzvidzo O, Wheeler J, Govender K, Iacuone S, Thompson P, et al. The phytosulfokine (PSK) receptor is capable of guanylate cyclase activity and enabling cyclic GMP-dependent signaling in plants. J Biol Chem. 2011;286:22580-8 pubmed publisher
    ..Overexpression of the full-length AtPSKR1 receptor in Arabidopsis leaf protoplasts raised the endogenous basal cGMP levels over 20-fold, indicating that the receptor has GC activity in vivo...
  22. Lee D, Hwang I. Transient expression and analysis of chloroplast proteins in Arabidopsis protoplasts. Methods Mol Biol. 2011;774:59-71 pubmed publisher
    ..Recently, an in vivo targeting method using protoplasts emerged as an alternative method to investigate protein targeting into chloroplasts...
  23. Bandmann V, Homann U. Clathrin-independent endocytosis contributes to uptake of glucose into BY-2 protoplasts. Plant J. 2012;70:578-84 pubmed publisher
    ..These data demonstrate that clathrin-independent endocytosis does occur in plant cells. This pathway may represent a common mechanism for the uptake of external nutrients...
  24. Wolfenstetter S, Wirsching P, Dotzauer D, Schneider S, Sauer N. Routes to the tonoplast: the sorting of tonoplast transporters in Arabidopsis mesophyll protoplasts. Plant Cell. 2012;24:215-32 pubmed publisher
    ..responsible for the sorting of higher plant INT1-type transporters to the tonoplast in Arabidopsis mesophyll protoplasts. We demonstrate that this motif can reroute other proteins, such as INT4, SUCROSE TRANSPORTER2 (SUC2), or ..
  25. Xiao L, Zhang L, Yang G, Zhu H, He Y. Transcriptome of protoplasts reprogrammed into stem cells in Physcomitrella patens. PLoS ONE. 2012;7:e35961 pubmed publisher
    ..However, the molecular mechanisms underlying the reprogramming of protoplasts into stem cells remain largely unknown...
  26. Wege S, Jossier M, Filleur S, Thomine S, Barbier Brygoo H, Gambale F, et al. The proline 160 in the selectivity filter of the Arabidopsis NO(3)(-)/H(+) exchanger AtCLCa is essential for nitrate accumulation in planta. Plant J. 2010;63:861-9 pubmed publisher
    ..Our results confirm the significance of this amino acid in the conserved selectivity filter of CLC proteins and highlight the importance of the proline in AtCLCa for nitrate metabolism in Arabidopsis...
  27. Cho Y, Yoo S. Expression of epitope-tagged proteins in Arabidopsis leaf mesophyll protoplasts. Methods Mol Biol. 2010;657:33-42 pubmed publisher
    ..The leaf mesophyll protoplasts (LMP) provide a perfect system to transiently express a gene encoding an epitope-tagged protein of interest and ..
  28. Robert S, Zouhar J, Carter C, Raikhel N. Isolation of intact vacuoles from Arabidopsis rosette leaf-derived protoplasts. Nat Protoc. 2007;2:259-62 pubmed
    ..This method is based on osmotic and thermal disruption of mesophyl-derived Arabidopsis protoplasts, followed by a density gradient fractionation of the cellular content...
  29. Niemes S, Langhans M, Viotti C, Scheuring D, San Wan Yan M, Jiang L, et al. Retromer recycles vacuolar sorting receptors from the trans-Golgi network. Plant J. 2010;61:107-21 pubmed publisher
  30. D Onofrio C, Lindberg S. Sodium induces simultaneous changes in cytosolic calcium and pH in salt-tolerant quince protoplasts. J Plant Physiol. 2009;166:1755-63 pubmed publisher
    ..Mill.) have shown that this cultivar takes up sodium transiently into the cytosol of shoot protoplasts only in the absence of calcium chloride, or at <1mM calcium chloride...
  31. Wang J, Turina M, Medina V, Falk B. Synergistic interaction between the Potyvirus, Turnip mosaic virus and the Crinivirus, Lettuce infectious yellows virus in plants and protoplasts. Virus Res. 2009;144:163-70 pubmed publisher
    ..LIYV infections result in a low virus titer in plants and protoplasts, impeding reverse genetic efforts to analyze LIYV gene/protein functions...
  32. Ondrej V, Kitner M, Doležalová I, Nádvorník P, Navrátilová B, Lebeda A. Chromatin structural rearrangement during dedifferentiation of protoplasts of Cucumis sativus L. Mol Cells. 2009;27:443-7 pubmed publisher
    ..of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus)...
  33. Gitzinger M, Parsons J, Reski R, Fussenegger M. Functional cross-kingdom conservation of mammalian and moss (Physcomitrella patens) transcription, translation and secretion machineries. Plant Biotechnol J. 2009;7:73-86 pubmed publisher
    ..patens protoplasts cultivated in a Wave Bioreactor...
  34. Karran R, Hudak K. Depurination within the intergenic region of Brome mosaic virus RNA3 inhibits viral replication in vitro and in vivo. Nucleic Acids Res. 2008;36:7230-9 pubmed publisher
    ..RNAs with PAP inhibited their translation in a cell-free system and decreased their accumulation in barley protoplasts. In the current study, we map the depurination sites on BMV RNA3 and describe the mechanism by which ..
  35. Cummins I, Steel P, Edwards R. Identification of a carboxylesterase expressed in protoplasts using fluorescence-activated cell sorting. Plant Biotechnol J. 2007;5:354-9 pubmed
    ..b>Protoplasts of Arabidopsis thaliana were prepared and transfected with a mutated (Cys59Ser) Arabidopsis S-formylglutathione ..
  36. Tessadori F, Chupeau M, Chupeau Y, Knip M, Germann S, van Driel R, et al. Large-scale dissociation and sequential reassembly of pericentric heterochromatin in dedifferentiated Arabidopsis cells. J Cell Sci. 2007;120:1200-8 pubmed
    ..Here we show that dedifferentiation of specialized mesophyll cells into undifferentiated protoplasts is accompanied by the disruption of chromocenter structures...
  37. Sheahan M, Rose R, McCurdy D. Actin-filament-dependent remodeling of the vacuole in cultured mesophyll protoplasts. Protoplasma. 2007;230:141-52 pubmed
    ..Here we examined the changes to vacuolar architecture in Arabidopsis thaliana protoplasts expressing a green-fluorescent protein fusion to a delta-tonoplast-intrinsic protein (deltaTIP)...
  38. Choi K, Park C, Lee J, Oh M, Noh B, Lee I. Arabidopsis homologs of components of the SWR1 complex regulate flowering and plant development. Development. 2007;134:1931-41 pubmed
    ..Our results strongly support the presence of an SWR1C-like complex in Arabidopsis that ensures proper development, including floral repression through full activation of FLC...
  39. Miao Y, Jiang L. Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells. Nat Protoc. 2007;2:2348-53 pubmed
    ..expression system to study the subcellular localization and dynamic associations of plant proteins using protoplasts freshly prepared from Arabidopsis or tobacco BY-2 suspension cultured cells...
  40. Thieme R, Rakosy Tican E, Gavrilenko T, Antonova O, Schubert J, Nachtigall M, et al. Novel somatic hybrids (Solanum tuberosum L.+Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight. Theor Appl Genet. 2008;116:691-700 pubmed publisher
    ..Somatic hybridization again proved to be a valuable tool for producing pre-breeding material with increased genetic diversity...
  41. Sommer A, Geist B, Da Ines O, Gehwolf R, Schäffner A, Obermeyer G. Ectopic expression of Arabidopsis thaliana plasma membrane intrinsic protein 2 aquaporins in lily pollen increases the plasma membrane water permeability of grain but not of tube protoplasts. New Phytol. 2008;180:787-97 pubmed publisher
    ..and single-cell RT-PCR, and by measuring the water permeability coefficient (P(os)) in swelling assays using protoplasts prepared from transformed pollen grains and tubes...
  42. Zhai Z, Sooksa nguan T, Vatamaniuk O. Establishing RNA interference as a reverse-genetic approach for gene functional analysis in protoplasts. Plant Physiol. 2009;149:642-52 pubmed publisher
    ..This study demonstrated that RNAi can also be utilized to examine gene functions in protoplasts. Because protoplasts are nongrowing cells, effective RNAi-triggered gene silencing depends not only on a ..
  43. Chen X, Wei P, Fan L, Yang D, Zhu X, Shen W, et al. Generation of high-yield rapamycin-producing strains through protoplasts-related techniques. Appl Microbiol Biotechnol. 2009;83:507-12 pubmed publisher
    ..In the present work, several protoplasts-related techniques including protoplasts mutation, intraspecies and interspecies protoplasts fusion were tried ..
  44. Xu T, Qu Z, Yang X, Qin X, Xiong J, Wang Y, et al. A cotton kinesin GhKCH2 interacts with both microtubules and microfilaments. Biochem J. 2009;421:171-80 pubmed publisher
    ..A GFP-fusion protein GFP-GhKCH2 transiently overexpressed in Arabidopsis protoplasts decorated both microtubules and microfilaments, confirming the binding ability and specificities of GhKCH2 on ..
  45. Wang C, Zhang X, Chen Z, Wen Y, Song Y. Strain construction for enhanced production of spinosad via intergeneric protoplast fusion. Can J Microbiol. 2009;55:1070-5 pubmed publisher
    ..Protoplast fusion was conducted with UV-irradiated protoplasts of S. spinosa and S. avermitilis...
  46. Fujii H, Chinnusamy V, Rodrigues A, Rubio S, Antoni R, Park S, et al. In vitro reconstitution of an abscisic acid signalling pathway. Nature. 2009;462:660-4 pubmed publisher
    ..Introduction of these four components into plant protoplasts results in ABA-responsive gene expression...
  47. Dinakar C, Abhaypratap V, Yearla S, Raghavendra A, Padmasree K. Importance of ROS and antioxidant system during the beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation. Planta. 2010;231:461-74 pubmed publisher
    ..Changes in steady-state photosynthesis of pea mesophyll protoplasts monitored in the presence of antimycin A [AA, inhibitor of cytochrome oxidase (COX) pathway] and ..
  48. Chamberlain K, Marshall R, Jolliffe N, Frigerio L, Ceriotti A, Lord J, et al. Ricin B chain targeted to the endoplasmic reticulum of tobacco protoplasts is degraded by a CDC48- and vacuole-independent mechanism. J Biol Chem. 2008;283:33276-86 pubmed publisher
    The B chain of ricin was expressed and delivered to the endoplasmic reticulum of tobacco protoplasts where it disappeared with time in a manner consistent with degradation. This turnover did not occur in the vacuoles or upon secretion...
  49. Marshall R, Jolliffe N, Ceriotti A, Snowden C, Lord J, Frigerio L, et al. The role of CDC48 in the retro-translocation of non-ubiquitinated toxin substrates in plant cells. J Biol Chem. 2008;283:15869-77 pubmed publisher
    ..denoted as RTA and RCA A, respectively) are delivered into the endoplasmic reticulum (ER) of tobacco protoplasts, they become substrates for ER-associated protein degradation (ERAD)...
  50. Miura K, Jin J, Lee J, Yoo C, Stirm V, Miura T, et al. SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis. Plant Cell. 2007;19:1403-14 pubmed
    ..A K393R substitution in ICE1 [ICE1(K393R)] blocked SIZ1-mediated sumoylation in vitro and in protoplasts identifying the K393 residue as the principal site of SUMO conjugation...
  51. Chen I, Lin J, Chen Y, Wang Z, Liang L, Meng M, et al. The 3'-terminal sequence of Bamboo mosaic virus minus-strand RNA interacts with RNA-dependent RNA polymerase and initiates plus-strand RNA synthesis. Mol Plant Pathol. 2010;11:203-12 pubmed publisher
    ..required and the changes at the penultimate U are deleterious to viral accumulation in Nicotiana benthamiana protoplasts. Results from UV-crosslinking and in vitro RNA-dependent RNA polymerase competition assays suggested that the ..
  52. Yokota Y, Yamada S, Hase Y, Shikazono N, Narumi I, Tanaka A, et al. Initial yields of DNA double-strand breaks and DNA Fragmentation patterns depend on linear energy transfer in tobacco BY-2 protoplasts irradiated with helium, carbon and neon ions. Radiat Res. 2007;167:94-101 pubmed
    ..In this study, DNA double-strand breaks (DSBs) were quantified by a DNA fragment-size analysis in tobacco protoplasts irradiated with high-LET ions...
  53. Hu X, Jiang M, Zhang J, Zhang A, Lin F, Tan M. Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants. New Phytol. 2007;173:27-38 pubmed
    ..Treatment with ABA or H(2)O(2) led to significant increases in the concentration of cytosolic Ca(2+) in the protoplasts of mesophyll cells and in the expression of the calmodulin 1 (CaM1) gene and the content of CaM in leaves of ..