eukaryotic cells

Summary

Summary: Cells of the higher organisms, containing a true nucleus bounded by a nuclear membrane.

Top Publications

  1. Labib K. How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells?. Genes Dev. 2010;24:1208-19 pubmed publisher
    Chromosome replication occurs precisely once during the cell cycle of almost all eukaryotic cells, and is a highly complex process that is still understood relatively poorly...
  2. Field M, Sali A, Rout M. Evolution: On a bender--BARs, ESCRTs, COPs, and finally getting your coat. J Cell Biol. 2011;193:963-72 pubmed publisher
    ..These new insights have had a profound impact on our understanding of the evolutionary origins of the internal architecture of the eukaryotic cell. ..
  3. Lane N. Energetics and genetics across the prokaryote-eukaryote divide. Biol Direct. 2011;6:35 pubmed publisher
    All complex life on Earth is eukaryotic. All eukaryotic cells share a common ancestor that arose just once in four billion years of evolution...
  4. Francis O, Han F, Adams J. Molecular phylogeny of a RING E3 ubiquitin ligase, conserved in eukaryotic cells and dominated by homologous components, the muskelin/RanBPM/CTLH complex. PLoS ONE. 2013;8:e75217 pubmed publisher
    ..is an essential post-translational modification that regulates signalling and protein turnover in eukaryotic cells. Specificity of ubiquitination is driven by ubiquitin E3 ligases, many of which remain poorly understood...
  5. Risco C, Sanmartín Conesa E, Tzeng W, Frey T, Seybold V, de Groot R. Specific, sensitive, high-resolution detection of protein molecules in eukaryotic cells using metal-tagging transmission electron microscopy. Structure. 2012;20:759-66 pubmed publisher
    ..The applicability and strength of METTEM is demonstrated by a study of Rubella virus replicase and capsid proteins, which revealed virus-induced cell structures not seen before...
  6. Cai H, Devreotes P. Moving in the right direction: how eukaryotic cells migrate along chemical gradients. Semin Cell Dev Biol. 2011;22:834-41 pubmed publisher
    ..Recent studies in Dictyostelium discoideum and mammalian neutrophils have revealed a complex signaling network that enables cells to migrate in chemical gradients...
  7. McIntosh K, Bhattacharya A, Willis I, Warner J. Eukaryotic cells producing ribosomes deficient in Rpl1 are hypersensitive to defects in the ubiquitin-proteasome system. PLoS ONE. 2011;6:e23579 pubmed publisher
    It has recently become clear that the misassembly of ribosomes in eukaryotic cells can have deleterious effects that go far beyond a simple shortage of ribosomes...
  8. Wickstead B, Gull K. The evolution of the cytoskeleton. J Cell Biol. 2011;194:513-25 pubmed publisher
    ..Much of this complexity evolved before the last common ancestor of eukaryotes. The distribution of cytoskeletal filaments puts constraints on the likely prokaryotic line that made this leap of eukaryogenesis...
  9. Smoyer C, Jaspersen S. Breaking down the wall: the nuclear envelope during mitosis. Curr Opin Cell Biol. 2014;26:1-9 pubmed publisher
    A defining feature of eukaryotic cells is the nucleus, which houses the genome inside the nuclear envelope (NE): a double lipid bilayer that separates the nuclear and cytoplasmic materials...

More Information

Publications62

  1. Jockusch B, Graumann P. The long journey: actin on the road to pro- and eukaryotic cells. Rev Physiol Biochem Pharmacol. 2011;161:67-85 pubmed publisher
    ..Various members have been identified in prokaryotic and eukaryotic cells, e.g. MreB, ParM and Ta0583, and actin and the actin-related proteins, ARPs, respectively...
  2. Parfrey L, Lahr D, Knoll A, Katz L. Estimating the timing of early eukaryotic diversification with multigene molecular clocks. Proc Natl Acad Sci U S A. 2011;108:13624-9 pubmed publisher
    ..In combination, paleontological and molecular approaches indicate that long stems preceded diversification in the major eukaryotic lineages...
  3. Araki H. Initiation of chromosomal DNA replication in eukaryotic cells; contribution of yeast genetics to the elucidation. Genes Genet Syst. 2011;86:141-9 pubmed
    ..molecular mechanism of DNA replication has been studied for a long time, knowledge regarding this process in eukaryotic cells has advanced rapidly in the past 20 years. Yeast genetics contributed profoundly to this rapid advancement...
  4. Klute M, Melancon P, Dacks J. Evolution and diversity of the Golgi. Cold Spring Harb Perspect Biol. 2011;3:a007849 pubmed publisher
    ..the repertoire, processes, and level of complexity of membrane-trafficking machinery present in early eukaryotic cells. This article serves as a review of the literature on the topic of Golgi evolution and diversity and reports ..
  5. Ferretti A, Larocca M, Favre C. Nutritional stress in eukaryotic cells: oxidative species and regulation of survival in time of scarceness. Mol Genet Metab. 2012;105:186-92 pubmed publisher
    The survival response to glucose limitation in eukaryotic cells involves different signaling pathways highly conserved from yeasts to mammals...
  6. Elias M, Brighouse A, Gabernet Castello C, Field M, Dacks J. Sculpting the endomembrane system in deep time: high resolution phylogenetics of Rab GTPases. J Cell Sci. 2012;125:2500-8 pubmed publisher
    The presence of a nucleus and other membrane-bounded intracellular compartments is the defining feature of eukaryotic cells. Endosymbiosis accounts for the origins of mitochondria and plastids, but the evolutionary ancestry of the ..
  7. Rigort A, Bäuerlein F, Villa E, Eibauer M, Laugks T, Baumeister W, et al. Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography. Proc Natl Acad Sci U S A. 2012;109:4449-54 pubmed publisher
    ..with today's intermediate voltage electron microscopes only small prokaryotic cells or peripheral regions of eukaryotic cells can be examined directly...
  8. Miller J, Reese J. Ccr4-Not complex: the control freak of eukaryotic cells. Crit Rev Biochem Mol Biol. 2012;47:315-33 pubmed publisher
  9. Serricchio M, Bütikofer P. An essential bacterial-type cardiolipin synthase mediates cardiolipin formation in a eukaryote. Proc Natl Acad Sci U S A. 2012;109:E954-61 pubmed publisher
    ..During depletion of cardiolipin synthase, the levels of cytochrome oxidase subunit IV and cytochrome c1, reflecting mitochondrial respiratory complexes IV and III, respectively, decreased progressively...
  10. Nagant C, Savage P, Dehaye J. Effect of pluronic acid F-127 on the toxicity towards eukaryotic cells of CSA-13, a cationic steroid analogue of antimicrobial peptides. J Appl Microbiol. 2012;112:1173-83 pubmed publisher
    CSA-13 is an antimicrobial cationic steroid with some toxicity against eukaryotic cells. The purpose of this work was to test whether pluronic acid F-127 could interfere with the toxicity of CSA-13 on human umbilical vein endothelial (..
  11. Senoh M, Ghosh Banerjee J, Ramamurthy T, Colwell R, Miyoshi S, Nair G, et al. Conversion of viable but nonculturable enteric bacteria to culturable by co-culture with eukaryotic cells. Microbiol Immunol. 2012;56:342-5 pubmed publisher
    ..coli, enteropathogenic E. coli, Shigella flexneri, and Salmonella enterica were converted to the culturable state by co-culture with selected eukaryotic cells, e.g., HT-29, Caco-2, T84, HeLa, Intestine 407, and CHO cells.
  12. Grumezescu A, Andronescu E, Ficai A, Bleotu C, Mihaiescu D, Chifiriuc M. Synthesis, characterization and in vitro assessment of the magnetic chitosan-carboxymethylcellulose biocomposite interactions with the prokaryotic and eukaryotic cells. Int J Pharm. 2012;436:771-7 pubmed publisher
    ..crystallinity, and the in vitro efficacy as antibiotic delivery vehicles as well as their influence on the eukaryotic cells are reported...
  13. Kulak N, Pichler G, Paron I, Nagaraj N, Mann M. Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells. Nat Methods. 2014;11:319-24 pubmed publisher
    ..97). The in-StageTip method is straightforward and generally applicable in biological or clinical applications. ..
  14. Jilkine A, Edelstein Keshet L. A comparison of mathematical models for polarization of single eukaryotic cells in response to guided cues. PLoS Comput Biol. 2011;7:e1001121 pubmed publisher
  15. Martin Galiano A, Oliva M, Sanz L, Bhattacharyya A, Serna M, Yébenes H, et al. Bacterial tubulin distinct loop sequences and primitive assembly properties support its origin from a eukaryotic tubulin ancestor. J Biol Chem. 2011;286:19789-803 pubmed publisher
  16. Amiri R, Yur eva N, Shimshilashvili K, Goldenkova Pavlova I, Pchelkin V, Kuznitsova E, et al. Expression of acyl-lipid Delta12-desaturase gene in prokaryotic and eukaryotic cells and its effect on cold stress tolerance of potato. J Integr Plant Biol. 2010;52:289-97 pubmed publisher
  17. Price C, Al Quadan T, Santic M, Jones S, Abu Kwaik Y. Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila. J Exp Med. 2010;207:1713-26 pubmed publisher
    ..In conclusion, this study shows novel localized recruitment of the host farnesylation machinery and its anchoring of an F-box effector to the LCV membrane, and this is essential for biological function in vitro and in vivo...
  18. Javaux E, Marshall C, Bekker A. Organic-walled microfossils in 3.2-billion-year-old shallow-marine siliciclastic deposits. Nature. 2010;463:934-8 pubmed publisher
    ..Our observations suggest that relatively large microorganisms cohabited with earlier reported benthic microbial mats in the photic zone of marginal marine siliciclastic environments 3.2 billion years ago...
  19. Li F, Tian L, Gu L, Li G. Evidence that nucleosomes inhibit mismatch repair in eukaryotic cells. J Biol Chem. 2009;284:33056-61 pubmed publisher
    ..These findings suggest that nucleosomes may inhibit MMR in eukaryotic cells. The implications of these findings for our understanding of eukaryotic MMR are discussed.
  20. Ochsenkühn M, Jess P, Stoquert H, Dholakia K, Campbell C. Nanoshells for surface-enhanced Raman spectroscopy in eukaryotic cells: cellular response and sensor development. ACS Nano. 2009;3:3613-21 pubmed publisher
    ..We demonstrate its application using 4-mercaptobenzoic acid (4-MBA)-functionalized nanoshells as a pH sensor...
  21. Fernandez Pol J. Conservation of multifunctional ribosomal protein metallopanstimulin-1 (RPS27) through complex evolution demonstrates its key role in growth regulation in Archaea, eukaryotic cells, DNA repair, translation and viral replication. Cancer Genomics Proteomics. 2011;8:105-26 pubmed
    ..Thus, p53, RPS27L, and RPS27/MPS-1) regulate growth and survival...
  22. Wang W, Geng X, Dou Y, Liu T, Zheng X. Predicting protein subcellular localization by pseudo amino acid composition with a segment-weighted and features-combined approach. Protein Pept Lett. 2011;18:480-7 pubmed
    ..The results indicate that the prediction quality by our method is quite satisfactory. It is anticipated that the current method may serve as an alternative approach to the existing prediction methods...
  23. Blombach F, Makarova K, Marrero J, Siebers B, Koonin E, van der Oost J. Identification of an ortholog of the eukaryotic RNA polymerase III subunit RPC34 in Crenarchaeota and Thaumarchaeota suggests specialization of RNA polymerases for coding and non-coding RNAs in Archaea. Biol Direct. 2009;4:39 pubmed publisher
    ..These findings suggest that functional separation of RNA polymerases for protein-coding genes and non-coding RNAs might predate the origin of the Eukaryotes...
  24. Mowbrey K, Dacks J. Evolution and diversity of the Golgi body. FEBS Lett. 2009;583:3738-45 pubmed publisher
  25. Yoshida K, Sugimoto N, Iwahori S, Yugawa T, Narisawa Saito M, Kiyono T, et al. CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells. J Cell Sci. 2010;123:225-35 pubmed publisher
    ..Thus, CDC6 might regulate replication-checkpoint activation through the interaction with ATR in higher eukaryotic cells.
  26. Neumann N, Lundin D, Poole A. Comparative genomic evidence for a complete nuclear pore complex in the last eukaryotic common ancestor. PLoS ONE. 2010;5:e13241 pubmed publisher
    ..Nups are broadly conserved between yeast, vertebrates and plants, but few have been identified among other major eukaryotic groups...
  27. O Malley M. The first eukaryote cell: an unfinished history of contestation. Stud Hist Philos Biol Biomed Sci. 2010;41:212-24 pubmed publisher
    ..My examination of these issues will conclude with broader reflections on the implications of eukaryogenesis studies for a philosophical understanding of scientific contestation...
  28. Mader A, Elad N, Medalia O. Cryoelectron tomography of eukaryotic cells. Methods Enzymol. 2010;483:245-65 pubmed publisher
    ..The development of cryoelectron tomography (cryo-ET) and its application to eukaryotic cells has provided a major step forward toward better realizing these processes...
  29. Elias M. Patterns and processes in the evolution of the eukaryotic endomembrane system. Mol Membr Biol. 2010;27:469-89 pubmed publisher
    ..Finally, reductive evolution and recurrent gene loss are emerging as pervasive factors shaping the ES in all phylogenetic lineages...
  30. Takahashi H, Shimada I. Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells. J Biomol NMR. 2010;46:3-10 pubmed publisher
    ..In this paper, we review the recent advances in expression systems for isotopically labeled heterologous proteins, utilizing non-E. coli prokaryotic and eukaryotic cells.
  31. Jacquier A. The complex eukaryotic transcriptome: unexpected pervasive transcription and novel small RNAs. Nat Rev Genet. 2009;10:833-44 pubmed publisher
  32. Snook R, Harvey T, Correia Faria E, Gardner P. Raman tweezers and their application to the study of singly trapped eukaryotic cells. Integr Biol (Camb). 2009;1:43-52 pubmed publisher
    ..g.chromosomes and mitochondria are then discussed followed by a summary of the future potential of the technique for single cell analysis...
  33. McCutcheon J. The bacterial essence of tiny symbiont genomes. Curr Opin Microbiol. 2010;13:73-8 pubmed publisher
    ..New data from various systems with symbiotic bacterial or archaeal partners have begun to shed light on how these bacteria may function with such small gene sets, but major mechanistic questions remain...
  34. Cavalier Smith T. Origin of the cell nucleus, mitosis and sex: roles of intracellular coevolution. Biol Direct. 2010;5:7 pubmed publisher
    ..Discussions on the origin of sex typically overlook its association with protozoan entry into dormant walled cysts and the likely simultaneous coevolutionary, not sequential, origin of mitosis and meiosis...
  35. Hodges M, Scheumann N, Wickstead B, Langdale J, Gull K. Reconstructing the evolutionary history of the centriole from protein components. J Cell Sci. 2010;123:1407-13 pubmed publisher
    ..Finally, we demonstrate a correlation between the presence of specific centriolar proteins and eye evolution. This correlation is used to predict proteins with functions in the development of ciliary, but not rhabdomeric, eyes...
  36. Bolte K, Gruenheit N, Felsner G, Sommer M, Maier U, Hempel F. Making new out of old: recycling and modification of an ancient protein translocation system during eukaryotic evolution. Mechanistic comparison and phylogenetic analysis of ERAD, SELMA and the peroxisomal importomer. Bioessays. 2011;33:368-76 pubmed publisher
    ..Editor's suggested further reading in BioEssays ERAD ubiquitin ligases Abstract...
  37. Lane N, Martin W. The energetics of genome complexity. Nature. 2010;467:929-34 pubmed publisher
    ..This vast leap in genomic capacity was strictly dependent on mitochondrial power, and prerequisite to eukaryote complexity: the key innovation en route to multicellular life...
  38. Duszenko M, Ginger M, Brennand A, Gualdrón López M, Colombo M, Coombs G, et al. Autophagy in protists. Autophagy. 2011;7:127-58 pubmed
    Autophagy is the degradative process by which eukaryotic cells digest their own components using acid hydrolases within the lysosome...
  39. Pierleoni A, Martelli P, Casadio R. MemLoci: predicting subcellular localization of membrane proteins in eukaryotes. Bioinformatics. 2011;27:1224-30 pubmed publisher
    ..This is so particularly for membrane proteins. Furthermore, most of the available predictors of subcellular localization are specifically trained on globular proteins and poorly perform on membrane proteins...
  40. Aktas M, Wessel M, Hacker S, Klüsener S, Gleichenhagen J, Narberhaus F. Phosphatidylcholine biosynthesis and its significance in bacteria interacting with eukaryotic cells. Eur J Cell Biol. 2010;89:888-94 pubmed publisher
    ..In the Pcs pathway, choline is condensed directly with CDP-diacylglycerol to form PC. This review focuses on the biosynthetic pathways and the significance of PC in bacteria with an emphasis on plant-microbe interactions...
  41. Cotton J, McInerney J. Eukaryotic genes of archaebacterial origin are more important than the more numerous eubacterial genes, irrespective of function. Proc Natl Acad Sci U S A. 2010;107:17252-5 pubmed publisher
    ..life shows eukaryotes as a distinct lineage of living things, but many studies have suggested that the first eukaryotic cells were chimeric, descended from both Eubacteria (through the mitochondrion) and Archaebacteria...
  42. Cifra M, Pokorny J, Havelka D, Kucera O. Electric field generated by axial longitudinal vibration modes of microtubule. Biosystems. 2010;100:122-31 pubmed publisher
    ..The electric field generated by vibrations of electrically polar cellular structures is expected to play an important role in biological self-organization...
  43. Levin A, Hayouka Z, Friedler A, Loyter A. Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cells. Virus Genes. 2010;40:341-6 pubmed publisher
    ..Our results probably indicate that binding of excess amounts of the Rev protein or the NES peptide to the exportin receptor CRM1 results in cells' death...
  44. Itzkovitz S, Hodis E, Segal E. Overlapping codes within protein-coding sequences. Genome Res. 2010;20:1582-9 pubmed publisher
    ..Our results suggest that genomes may have evolved to encode extensive overlapping information within protein-coding regions...
  45. Doucet C, Talamas J, Hetzer M. Cell cycle-dependent differences in nuclear pore complex assembly in metazoa. Cell. 2010;141:1030-41 pubmed publisher
    ..Our results suggest that in organisms with open mitosis, NPCs assemble via two distinct mechanisms to accommodate cell cycle-dependent differences in NE topology...
  46. Doucet C, Hetzer M. Nuclear pore biogenesis into an intact nuclear envelope. Chromosoma. 2010;119:469-77 pubmed publisher
    ..the nuclear membrane, a double lipid bilayer that physically separates the nucleoplasm and cytoplasm of eukaryotic cells. New evidence suggests that the multiprotein nuclear pores also play a role in chromatin organization and ..
  47. Faurez F, Dory D, Henry A, Bougeard S, Jestin A. Replication efficiency of rolling-circle replicon-based plasmids derived from porcine circovirus 2 in eukaryotic cells. J Virol Methods. 2010;165:27-35 pubmed publisher
    ..In this study, a method was developed to measure replication rates of rolling-circle replicon-based plasmids in eukaryotic cells. This method is based on the discriminative quantitation of MboI-resistant, non-replicated input plasmids and ..
  48. Truong L, Wu X. Prevention of DNA re-replication in eukaryotic cells. J Mol Cell Biol. 2011;3:13-22 pubmed publisher
  49. Reis O, Sousa S, Camejo A, Villiers V, Gouin E, Cossart P, et al. LapB, a novel Listeria monocytogenes LPXTG surface adhesin, required for entry into eukaryotic cells and virulence. J Infect Dis. 2010;202:551-62 pubmed publisher
    ..Our results highlight LapB as a new L. monocytogenes virulence adhesin with a function that is supported by its unique N-terminal domain through the probable interaction with a cellular receptor...
  50. Gould C, Diella F, Via A, Puntervoll P, Gemund C, Chabanis Davidson S, et al. ELM: the status of the 2010 eukaryotic linear motif resource. Nucleic Acids Res. 2010;38:D167-80 pubmed publisher
    ..Using the links, researchers can explore the motifs, proteins, complex structures and associated literature to evaluate whether candidate motifs might be worth experimental investigation...
  51. Arbeloa A, Oates C, Marchès O, Hartland E, Frankel G. Enteropathogenic and enterohemorrhagic Escherichia coli type III secretion effector EspV induces radical morphological changes in eukaryotic cells. Infect Immun. 2011;79:1067-76 pubmed publisher
    ..Although the role of EspV in infection and its target host cell protein(s) require further investigation, the data point to a novel mechanism by which the T3SS subverts cell signaling...
  52. Corinaldesi C, Barucca M, Luna G, Dell Anno A. Preservation, origin and genetic imprint of extracellular DNA in permanently anoxic deep-sea sediments. Mol Ecol. 2011;20:642-54 pubmed publisher
  53. Wente S, Rout M. The nuclear pore complex and nuclear transport. Cold Spring Harb Perspect Biol. 2010;2:a000562 pubmed publisher
    Internal membrane bound structures sequester all genetic material in eukaryotic cells. The most prominent of these structures is the nucleus, which is bounded by a double membrane termed the nuclear envelope (NE)...