ameloblasts

Summary

Summary: Cylindrical epithelial cells in the innermost layer of the ENAMEL ORGAN. Their functions include contribution to the development of the dentinoenamel junction by the deposition of a layer of the matrix, thus producing the foundation for the prisms (the structural units of the DENTAL ENAMEL), and production of the matrix for the enamel prisms and interprismatic substance. (From Jablonski's Dictionary of Dentistry, 1992)

Top Publications

  1. Biz M, Marques M, Crema V, Moriscot A, dos Santos M. GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts. Cell Tissue Res. 2010;340:459-70 pubmed publisher
    ..The aim of this study was to verify the expression, distribution, and role of Rho GTPases in ameloblasts and odontoblasts during tooth development in correlation with actin and tubulin arrangements and amelogenin and ..
  2. Kestler D, Foster J, Macy S, Murphy C, Weiss D, Solomon A. Expression of odontogenic ameloblast-associated protein (ODAM) in dental and other epithelial neoplasms. Mol Med. 2008;14:318-26 pubmed publisher
    ..Notably, these reagents immunostained normal and malignant ameloblasts and other types of human neoplastic cells, including those of gastric, lung, and breast origin where the ..
  3. Ning F, Guo Y, Tang J, Zhou J, Zhang H, Lu W, et al. Differentiation of mouse embryonic stem cells into dental epithelial-like cells induced by ameloblasts serum-free conditioned medium. Biochem Biophys Res Commun. 2010;394:342-7 pubmed publisher
    ..By culturing mESCs in ameloblasts serum-free conditioned medium (ASF-CM), we could induce their differentiation toward dental epithelial cell ..
  4. Wang X, Suzawa T, Ohtsuka H, Zhao B, Miyamoto Y, Miyauchi T, et al. Carbonic anhydrase II regulates differentiation of ameloblasts via intracellular pH-dependent JNK signaling pathway. J Cell Physiol. 2010;225:709-19 pubmed publisher
    Differentiation of ameloblasts from undifferentiated epithelial cells is controlled by diverse growth factors, as well as interactions between epithelium and mesenchyme...
  5. Simmer J, Papagerakis P, Smith C, Fisher D, Rountrey A, Zheng L, et al. Regulation of dental enamel shape and hardness. J Dent Res. 2010;89:1024-38 pubmed publisher
    ..Here we review the growth parameters that determine the shape of the enamel crown as well as the mechanisms of enamel appositional growth and maturation...
  6. Lyaruu D, Bronckers A, Mulder L, Mardones P, Medina J, Kellokumpu S, et al. The anion exchanger Ae2 is required for enamel maturation in mouse teeth. Matrix Biol. 2008;27:119-27 pubmed
    ..We tested the hypothesis that in ameloblasts the anion exchanger-2 (Ae2) is involved in pH regulation during maturation stage amelogenesis...
  7. Josephsen K, Takano Y, Frische S, Praetorius J, Nielsen S, Aoba T, et al. Ion transporters in secretory and cyclically modulating ameloblasts: a new hypothesis for cellular control of preeruptive enamel maturation. Am J Physiol Cell Physiol. 2010;299:C1299-307 pubmed publisher
    ..Connexin 43 (Cx-43) is found in papillary cells and ameloblasts, whereas Na(+)-K(+)-ATPase is heavily expressed during maturation in the papillary cell layer only...
  8. Athanassiou Papaefthymiou M, Kim D, Harbron L, Papagerakis S, Schnell S, Harada H, et al. Molecular and circadian controls of ameloblasts. Eur J Oral Sci. 2011;119 Suppl 1:35-40 pubmed publisher
    ..In addition, ameloblasts follow daily rhythms in their main activities (i.e. enamel protein secretion and enamel mineralization)...
  9. Yamakoshi Y, Richardson A, Núñez S, Yamakoshi F, Milkovich R, Hu J, et al. Enamel proteins and proteases in Mmp20 and Klk4 null and double-null mice. Eur J Oral Sci. 2011;119 Suppl 1:206-16 pubmed publisher
    ..These results support the importance of MMP20 during the secretory stage and of KLK4 during the maturation stage and show there is only limited functional redundancy for these enzymes...

More Information

Publications62

  1. Ruspita I, Miyoshi K, Muto T, Abe K, Horiguchi T, Noma T. Sp6 downregulation of follistatin gene expression in ameloblasts. J Med Invest. 2008;55:87-98 pubmed
    ..back to equivalent levels seen in control cells, indicating that Sp6 regulates follistatin gene expression in ameloblasts. Our findings demonstrate that the follistatin gene is one of the Sp6 target genes in ameloblasts and suggest ..
  2. Hu Y, Hu J, Smith C, Bartlett J, Simmer J. Kallikrein-related peptidase 4, matrix metalloproteinase 20, and the maturation of murine and porcine enamel. Eur J Oral Sci. 2011;119 Suppl 1:217-25 pubmed publisher
    ..A surprising finding was the observation of electron backscatter from mid-maturation wild-type ameloblasts, which we attributed to the accumulation and release of iron...
  3. Porto I, Merzel J, de Sousa F, Bachmann L, Cury J, Line S, et al. Enamel mineralization in the absence of maturation stage ameloblasts. Arch Oral Biol. 2009;54:313-21 pubmed publisher
    The role of maturation stage ameloblasts is not clear yet. The aim of this study was to verify to which extent enamel mineralizes in the absence of these cells...
  4. Iacob S, Veis A. Identification of temporal and spatial expression patterns of amelogenin isoforms during mouse molar development. Eur J Oral Sci. 2006;114 Suppl 1:194-200; discussion 201-2, 381 pubmed
    ..Polypeptides translated from several alternatively spliced isoforms of amelogenin mRNA have been identified in ameloblasts and odontoblasts...
  5. Michon F, Tummers M, Kyyrönen M, Frilander M, Thesleff I. Tooth morphogenesis and ameloblast differentiation are regulated by micro-RNAs. Dev Biol. 2010;340:355-68 pubmed publisher
    ..Based on our findings we suggest that miRNAs modulate tooth morphogenesis largely by fine tuning conserved signaling networks and that miRNAs may have played important roles during tooth evolution...
  6. Lacruz R, Hilvo M, Kurtz I, Paine M. A survey of carbonic anhydrase mRNA expression in enamel cells. Biochem Biophys Res Commun. 2010;393:883-7 pubmed publisher
    ..of the generation of bicarbonate by carbonic anhydrases (CA) has been suggested as one of the pathways used by ameloblasts cells to regulate extracellular pH yet only two isozymes (CA II and CA VI) have been reported to date during ..
  7. Nagano T, Oida S, Ando H, Gomi K, Arai T, Fukae M. Relative levels of mRNA encoding enamel proteins in enamel organ epithelia and odontoblasts. J Dent Res. 2003;82:982-6 pubmed
    ..These results support the conclusion that odontoblasts are involved in the formation of the enamel layer adjacent to enamel-dentin junction...
  8. Laurenti P, Thaëron C, Allizard F, Huysseune A, Sire J. Cellular expression of eve1 suggests its requirement for the differentiation of the ameloblasts and for the initiation and morphogenesis of the first tooth in the zebrafish (Danio rerio). Dev Dyn. 2004;230:727-33 pubmed
    ..Then, by 72 hpf, eve1 expression was restricted to the differentiating ameloblasts of the enamel organ during early differentiation stage, and this expression decreased as soon as matrix was ..
  9. Paine M, Zhu D, Luo W, Snead M. Overexpression of TRAP in the enamel matrix does not alter the enamel structural hierarchy. Cells Tissues Organs. 2004;176:7-16 pubmed
  10. Somogyi E, Petersson U, Hultenby K, Wendel M. Calreticulin--an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix. Matrix Biol. 2003;22:179-91 pubmed
    ..At the ultrastructural level, the labeling was distributed in the rat odontoblasts, ameloblasts and predentin...
  11. Goldberg M, Septier D. Phospholipids in amelogenesis and dentinogenesis. Crit Rev Oral Biol Med. 2002;13:276-90 pubmed
    ..Filipin, a probe for cholesterol, varied in density along the plasma membrane of secretory ameloblasts, and allowed us to visualize membrane remnants inside the forming enamel...
  12. Diekwisch T. The developmental biology of cementum. Int J Dev Biol. 2001;45:695-706 pubmed
    ..proteins were restricted to the crown enamel and were absent from the root surface and from the cervical-most ameloblasts adjacent to the root margin...
  13. Bloch Zupan A, Leveillard T, Gorry P, Fausser J, Ruch J. Expression of p21(WAF1/CIP1) during mouse odontogenesis. Eur J Oral Sci. 1998;106 Suppl 1:104-11 pubmed
    ..epithelium during late cap and initial bell stages and then confined to the post-mitotic odontoblasts and ameloblasts. The replicating cells were devoid of any signal...
  14. Lacruz R, Smith C, Kurtz I, Hubbard M, Paine M. New paradigms on the transport functions of maturation-stage ameloblasts. J Dent Res. 2013;92:122-9 pubmed publisher
    ..This review describes the main cellular activities of these genes during the maturation stage of amelogenesis...
  15. Chen S, Gluhak Heinrich J, Wang Y, Wu Y, Chuang H, Chen L, et al. Runx2, osx, and dspp in tooth development. J Dent Res. 2009;88:904-9 pubmed publisher
    ..In mouse odontoblast-like cells, Osx overexpression increased Dspp transcription. Analysis of these data suggests differential biological functions of Runx2, Osx, and Dspp during odontogenesis and osteogenesis...
  16. Gao Y, Li D, Han T, Sun Y, Zhang J. TGF-beta1 and TGFBR1 are expressed in ameloblasts and promote MMP20 expression. Anat Rec (Hoboken). 2009;292:885-90 pubmed publisher
    ..Both TGF-beta1 and TGFBR1 were strongly expressed in secreting ameloblasts. Next, we studied the effects of TGF-beta signaling on the expression of MMP20 and KLK4 mRNA using ameloblast-..
  17. Wang B, Li L, Du S, Liu C, Lin X, Chen Y, et al. Induction of human keratinocytes into enamel-secreting ameloblasts. Dev Biol. 2010;344:795-9 pubmed publisher
    ..FGF8, are induced to express the dental epithelial marker PITX2 and differentiate into enamel-secreting ameloblasts that develop a human-mouse chimeric whole tooth crown...
  18. Golonzhka O, Metzger D, Bornert J, Bay B, Gross M, Kioussi C, et al. Ctip2/Bcl11b controls ameloblast formation during mammalian odontogenesis. Proc Natl Acad Sci U S A. 2009;106:4278-83 pubmed publisher
    ..on the lingual aspect of mutant lower incisors, and the morphology, polarization, and adhesion properties of ameloblasts on the labial side of these teeth were severely disrupted...
  19. Caton J, Luder H, Zoupa M, Bradman M, Bluteau G, Tucker A, et al. Enamel-free teeth: Tbx1 deletion affects amelogenesis in rodent incisors. Dev Biol. 2009;328:493-505 pubmed publisher
    ..hypoplastic and lacked enamel, while thorough histological examinations demonstrated the complete absence of ameloblasts. The absence of enamel is preceded by a decrease in proliferation of the ameloblast precursor cells and a ..
  20. Duan X, Mao Y, Wen X, Yang T, Xue Y. Excess fluoride interferes with chloride-channel-dependent endocytosis in ameloblasts. J Dent Res. 2011;90:175-80 pubmed publisher
    ..We conclude that excess fluoride inhibits endocytic activity of ameloblasts through the CFTR chloride channel or other chloride channels...
  21. Asaka T, Akiyama M, Domon T, Nishie W, Natsuga K, Fujita Y, et al. Type XVII collagen is a key player in tooth enamel formation. Am J Pathol. 2009;174:91-100 pubmed publisher
    ..Col17(-/-) mouse incisors had poorly differentiated ameloblasts that lacked enamel protein-secreting Tomes' processes and reduced mRNA expression of amelogenin, ameloblastin, ..
  22. Nishikawa S, Fujiwara K, Kitamura H. Formation of the tooth enamel rod pattern and the cytoskeletal organization in secretory ameloblasts of the rat incisor. Eur J Cell Biol. 1988;47:222-32 pubmed
    ..of the rhodamine-phalloidin staining pattern of the distal terminal web and the enamel secretion pattern by ameloblasts revealed that a change in the distal terminal web staining pattern preceded a change in the secretion pattern...
  23. Terling C, Heymann R, Rozell B, Obrink B, Wroblewski J. Dynamic expression of E-cadherin in ameloblasts and cementoblasts in mice. Eur J Oral Sci. 1998;106 Suppl 1:137-42 pubmed
    ..E-cadherin was strongly expressed at the bell stage by the cells of the dental organ, and by the pre-secretory ameloblasts and the cells of stratum intermedium at the early mineralization stage...
  24. DenBesten P, Gao C, Li W, Mathews C, Gruenert D. Development and characterization of an SV40 immortalized porcine ameloblast-like cell line. Eur J Oral Sci. 1999;107:276-81 pubmed
    Enamel is secreted as a protein matrix by the ameloblasts. These same cells then control the maturation of the enamel matrix, secreting proteinases that hydrolyze proteins as mineralization progresses, until mature enamel containing less ..
  25. Oida S, Nagano T, Yamakoshi Y, Ando H, Yamada M, Fukae M. Amelogenin gene expression in porcine odontoblasts. J Dent Res. 2002;81:103-8 pubmed
    ..Amelogenin has been reported to be a specific secretory product of ameloblasts. In this study, we examined amelogenin gene expression in various cell layers prepared from a porcine permanent ..
  26. Li Y, Decker S, Yuan Z, DenBesten P, Aragon M, Jordan Sciutto K, et al. Effects of sodium fluoride on the actin cytoskeleton of murine ameloblasts. Arch Oral Biol. 2005;50:681-8 pubmed
    ..Since RhoA is a molecular switch, our hypothesis is that in ameloblasts, fluoride may alter the cytoskeleton through interference with the Rho signaling pathway...
  27. Nakata A, Kameda T, Nagai H, Ikegami K, Duan Y, Terada K, et al. Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line. Biochem Biophys Res Commun. 2003;308:834-9 pubmed
    Tooth development was cooperatively regulated by the epithelial ameloblasts and mesenchymal odontoblasts. Ameloblasts secrete enamel matrix, critical for enamel formation...
  28. Smith C, Chong D, Bartlett J, Margolis H. Mineral acquisition rates in developing enamel on maxillary and mandibular incisors of rats and mice: implications to extracellular acid loading as apatite crystals mature. J Bone Miner Res. 2005;20:240-9 pubmed
    ..Enamel organ cells (ameloblasts), therefore, likely excrete bicarbonate ions to prevent pH in fluid bathing enamel from becoming too acidic.
  29. Goldberg M, Septier D, Bourd K, Hall R, George A, Goldberg H, et al. Immunohistochemical localization of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the forming rat incisor. Connect Tissue Res. 2003;44:143-53 pubmed
    ..carried out on undemineralized material provided evidence for strong MMP-2 staining in the secretory ameloblasts, odontoblasts, in the enamel organ, and in the pulp...
  30. Wen X, Zou Y, Luo W, Goldberg M, Moats R, Conti P, et al. Biglycan overexpression on tooth enamel formation in transgenic mice. Anat Rec (Hoboken). 2008;291:1246-53 pubmed publisher
    ..These results suggested that increasing the biglycan expression, in the cells that synthesize the precursor protein matrix for enamel, has a negligible influence on amelogenesis...
  31. Cerny R, Slaby I, Hammarstrom L, Wurtz T. A novel gene expressed in rat ameloblasts codes for proteins with cell binding domains. J Bone Miner Res. 1996;11:883-91 pubmed
    Two variants of an mRNA sequence are identified that are expressed at high levels in rat ameloblasts during the formation of the enamel matrix. The sequences contain open reading frames for 407 and 324 amino acid residues, respectively...
  32. Simmer J, Richardson A, Smith C, Hu Y, Hu J. Expression of kallikrein-related peptidase 4 in dental and non-dental tissues. Eur J Oral Sci. 2011;119 Suppl 1:226-33 pubmed publisher
    ..After 5 h of incubation, X-gal staining was observed specifically in the nuclei of maturation-stage ameloblasts in molars and incisors from Klk4(lacZ/lacZ) mice and was detected weakly in the nuclei of salivary gland ducts ..
  33. Yoshiba K, Yoshiba N, Aberdam D, Meneguzzi G, Perrin Schmitt F, Stoetzel C, et al. Expression and localization of laminin-5 subunits during mouse tooth development. Dev Dyn. 1998;211:164-76 pubmed
    ..5), mRNAs of all three subunits were re-expressed by ameloblasts, and the corresponding proteins were detected in ameloblasts and in the enamel matrix...
  34. Seidel K, Ahn C, Lyons D, Nee A, Ting K, Brownell I, et al. Hedgehog signaling regulates the generation of ameloblast progenitors in the continuously growing mouse incisor. Development. 2010;137:3753-61 pubmed publisher
    ..we demonstrate that Hh signaling is not required for stem cell survival but is essential for the generation of ameloblasts, one of the major differentiated cell types in the tooth, from the stem cells...
  35. Risnes S, Septier D, Deville de Periere D, Goldberg M. TEM observations on the ameloblast/enamel interface in the rat incisor. Connect Tissue Res. 2002;43:496-504 pubmed
    The structure of rat incisor enamel is established at the topographically complex interface between secretory ameloblasts and forming enamel...
  36. Gluhak Heinrich J, Guo D, Yang W, Harris M, Lichtler A, Kream B, et al. New roles and mechanism of action of BMP4 in postnatal tooth cytodifferentiation. Bone. 2010;46:1533-45 pubmed publisher
    ..BMP signaling, as well as Dlx3 and Amelogenin expression, is also indirectly reduced in the ameloblasts of the odontoblast BMP4 cKO mice...
  37. Paine M, Snead M, Wang H, Abuladze N, Pushkin A, Liu W, et al. Role of NBCe1 and AE2 in secretory ameloblasts. J Dent Res. 2008;87:391-5 pubmed
    The H(+)/base transport processes that control the pH of the microenvironment adjacent to ameloblasts are not currently well-understood. Mice null for the AE2 anion exchanger have abnormal enamel...
  38. Takamori K, Hosokawa R, Xu X, Deng X, Bringas P, Chai Y. Epithelial fibroblast growth factor receptor 1 regulates enamel formation. J Dent Res. 2008;87:238-43 pubmed
    ..In K14-Cre;Fgfr1(fl/fl) mice, the apical sides of enamel-secreting ameloblasts failed to adhere properly to each other, although ameloblast differentiation was unaffected at early stages...
  39. Hu J, Hu Y, Smith C, McKee M, Wright J, Yamakoshi Y, et al. Enamel defects and ameloblast-specific expression in Enam knock-out/lacz knock-in mice. J Biol Chem. 2008;283:10858-71 pubmed publisher
    ..These results demonstrate ameloblast-specific expression of enamelin and reveal that enamelin is essential for proper enamel matrix organization and mineralization...
  40. Takahashi S, Kawashima N, Sakamoto K, Nakata A, Kameda T, Sugiyama T, et al. Differentiation of an ameloblast-lineage cell line (ALC) is induced by Sonic hedgehog signaling. Biochem Biophys Res Commun. 2007;353:405-11 pubmed
    ..These results suggest that Shh directly promotes ALC differentiation, and that Gli1 may be responsible for the signal mediation of Shh...
  41. Bronckers A, Kalogeraki L, Jorna H, Wilke M, Bervoets T, Lyaruu D, et al. The cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in maturation stage ameloblasts, odontoblasts and bone cells. Bone. 2010;46:1188-96 pubmed publisher
    ..We tested the hypothesis that Cftr channels are present and operating in the plasma membranes of mouse ameloblasts. Tissue sections of young mouse jaws and fetal human jaws were immunostained with various anti-Cftr antibodies...
  42. Yan Q, Zhang Y, Li W, DenBesten P. Differentiation of human ameloblast-lineage cells in vitro. Eur J Oral Sci. 2006;114 Suppl 1:154-8; discussion 164-5, 380-1 pubmed
    ..Immunofluorescence of the tooth bud sections showed staining for these matrix proteins as ameloblasts differentiated from the inner enamel epithelium...
  43. Bouwman P, Gollner H, Elsasser H, Eckhoff G, Karis A, Grosveld F, et al. Transcription factor Sp3 is essential for post-natal survival and late tooth development. EMBO J. 2000;19:655-61 pubmed
    ..Comparison of the Sp1 and Sp3 knockout phenotype shows that Sp1 and Sp3 have distinct functions in vivo, but also suggests a degree of functional redundancy...
  44. Obara N, Suzuki Y, Nagai Y, Takeda M. Expression of E- and P-cadherin during tooth morphogenesis and cytodifferentiation of ameloblasts. Anat Embryol (Berl). 1998;197:469-75 pubmed
    ..at the late bell stage when the cells of the inner enamel epithelium began to polarize to differentiate into ameloblasts, the polarizing ameloblasts lost P-cadherin and strongly expressed E-cadherin...
  45. Gomes J, Omar N, Dos Santos Neves J, Narvaes E, Novaes P. Immunolocalization and activity of the MMP-9 and MMP-2 in odontogenic region of the rat incisor tooth after post shortening procedure. J Mol Histol. 2011;42:153-9 pubmed publisher
  46. Tompkins K. Molecular mechanisms of cytodifferentiation in mammalian tooth development. Connect Tissue Res. 2006;47:111-8 pubmed
    ..Additionally, recent results suggest that phenotypic proteins of both ameloblasts and odontoblasts, such as amelogenin and dentin matrix protein 2 may act as the final instructive signals in ..
  47. Wazen R, Moffatt P, Zalzal S, Yamada Y, Nanci A. A mouse model expressing a truncated form of ameloblastin exhibits dental and junctional epithelium defects. Matrix Biol. 2009;28:292-303 pubmed publisher
    ..AMBN) is the second most abundant extracellular matrix protein produced by the epithelial cells called ameloblasts and is found mainly in forming dental enamel...
  48. Fukumoto S, Kiba T, Hall B, Iehara N, Nakamura T, Longenecker G, et al. Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts. J Cell Biol. 2004;167:973-83 pubmed
    ..During this process, epithelial cells differentiate into enamel-secreting ameloblasts. Ameloblastin, an enamel matrix protein, is expressed by differentiating ameloblasts...
  49. Hata M, Kawamoto T, Kawai M, Yamamoto T. Differential expression patterns of the tight junction-associated proteins occludin and claudins in secretory and mature ameloblasts in mouse incisor. Med Mol Morphol. 2010;43:102-6 pubmed publisher
    ..The CLD family consists of 24 members that show tissue- or cell-specific expression. Ameloblasts, which originate from the oral epithelium, form enamel, and enamel proteins and minerals are transported across ..
  50. Lacruz R, Nanci A, Kurtz I, Wright J, Paine M. Regulation of pH During Amelogenesis. Calcif Tissue Int. 2010;86:91-103 pubmed publisher
    ..This review summarizes the intra- and extracellular mechanisms employed by the enamel-forming cells, ameloblasts, to maintain pH homeostasis and, also, discusses the enamel phenotypes associated with disruptions to genes ..
  51. Notani T, Tabata M, Iseki H, Baba O, Takano Y. Introduction of a three-dimensional and layered (TDL) culture, a novel primary co-culture method for ameloblasts and pulp-derived cells. Arch Histol Cytol. 2009;72:187-98 pubmed
    The enamel organ engaged in enamel matrix formation in tooth germs comprises four different cell types: the ameloblasts, the cells of the stratum intermedium, stellate reticulum, and the outer enamel epithelium, each characterized by ..
  52. Okumura R, Shibukawa Y, Muramatsu T, Hashimoto S, Nakagawa K, Tazaki M, et al. Sodium-calcium exchangers in rat ameloblasts. J Pharmacol Sci. 2010;112:223-30 pubmed
    Although the central role of ameloblasts in synthesis and resorption of enamel matrix proteins during amelogenesis is well documented, the Ca(2+)-transport/extrusion mechanism remains to be fully elucidated...
  53. Wang X, Suomalainen M, Jorgez C, Matzuk M, Werner S, Thesleff I. Follistatin regulates enamel patterning in mouse incisors by asymmetrically inhibiting BMP signaling and ameloblast differentiation. Dev Cell. 2004;7:719-30 pubmed
    ..Enamel matrix is secreted by ameloblasts derived from dental epithelium...