llc pk1 cells


Summary: Epithelial cell line originally derived from porcine kidneys. It is used for pharmacologic and metabolic studies.

Top Publications

  1. Shimizu N, Petroni B, Khatri A, Gardella T. Functional evidence for an intramolecular side chain interaction between residues 6 and 10 of receptor-bound parathyroid hormone analogues. Biochemistry. 2003;42:2282-90 pubmed
  2. Fujita H, Omori S, Ishikura K, Hida M, Awazu M. ERK and p38 mediate high-glucose-induced hypertrophy and TGF-beta expression in renal tubular cells. Am J Physiol Renal Physiol. 2004;286:F120-6 pubmed
    ..These results demonstrate that ERK and p38 are activated in renal tubular cells of DM and may mediate HG-induced cellular hypertrophy and TGF-beta expression...
  3. Yano T, Itoh Y, Kubota T, Sendo T, Oishi R. A prostacyclin analog beraprost sodium attenuates radiocontrast media-induced LLC-PK1 cells injury. Kidney Int. 2004;65:1654-63 pubmed
    ..The present study was designed to determine whether the elevation of endogenous cAMP with beraprost sodium, a prostacyclin analog, reduces the contrast material-induced renal tubular injury...
  4. Lee E, Kim H, Cho M, Lee W, Lim S. Structure and function of a minimal receptor activation domain of parathyroid hormone. Yonsei Med J. 2004;45:419-27 pubmed
  5. Tulu U, Rusan N, Wadsworth P. Peripheral, non-centrosome-associated microtubules contribute to spindle formation in centrosome-containing cells. Curr Biol. 2003;13:1894-9 pubmed
    ..Our results directly demonstrate that non-centrosome-associated microtubules contribute to spindle formation in centrosome-containing cells...
  6. Piehl M, Tulu U, Wadsworth P, Cassimeris L. Centrosome maturation: measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1. Proc Natl Acad Sci U S A. 2004;101:1584-8 pubmed
  7. Ryan P, Bedard K, Breining T, Cribb A. Disruption of the endoplasmic reticulum by cytotoxins in LLC-PK1 cells. Toxicol Lett. 2005;159:154-63 pubmed
  8. Kallio M, Weinstein J, Daum J, Burke D, Gorbsky G. Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. J Cell Biol. 1998;141:1393-406 pubmed
    ..These studies suggest that mammalian p55CDC may be part of a regulatory and targeting complex for the anaphase-promoting complex...
  9. FOLSCH H, Ohno H, Bonifacino J, Mellman I. A novel clathrin adaptor complex mediates basolateral targeting in polarized epithelial cells. Cell. 1999;99:189-98 pubmed
    ..We conclude that basolateral sorting is mediated by an epithelial cell-specific version of the AP-1 complex containing mu1B...

More Information

Publications111 found, 100 shown here

  1. Rusan N, Fagerstrom C, Yvon A, Wadsworth P. Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin. Mol Biol Cell. 2001;12:971-80 pubmed
    ..Microtubule release from the centrosome and a subset of differentially stable astral microtubules were also observed. The results provide the first quantitative measurements of mitotic microtubule dynamics in mammalian cells. ..
  2. Gan Y, McGraw T, Rodriguez Boulan E. The epithelial-specific adaptor AP1B mediates post-endocytic recycling to the basolateral membrane. Nat Cell Biol. 2002;4:605-9 pubmed
    ..Targeting and recycling assays in LLC-PK1 cells, before and after transfection with mu 1B, and in MDCK cells, which express mu 1B constitutively, suggest that AP1B sorts basolateral proteins post-endocytically...
  3. Boecker W, Bernecker O, Wu J, Zhu X, Sawa T, Grazette L, et al. Cardiac-specific gene expression facilitated by an enhanced myosin light chain promoter. Mol Imaging. 2004;3:69-75 pubmed
    ..4 x MLC.Luc) versus 1:1.4 (Ad.CMV.Luc). This new vector may be useful to validate therapeutic approaches in animal disease models and offers the perspective for selective expression of therapeutic genes in the diseased heart. ..
  4. Wadsworth P, Rusan N, Tulu U, Fagerstrom C. Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells. Nat Methods. 2005;2:981-7 pubmed
  5. Wang X, Hu J, Price S. Inhibition of PI3-kinase signaling by glucocorticoids results in increased branched-chain amino acid degradation in renal epithelial cells. Am J Physiol Cell Physiol. 2007;292:C1874-9 pubmed
    ..Thus, glucocorticoids inhibit signaling through the IRS-1/PI3K/Akt pathway with a consequence of increased branched-chain amino acid catabolism. ..
  6. Towndrow K, Jia Z, Lo H, Person M, Monks T, Lau S. 11-Deoxy,16,16-dimethyl prostaglandin E2 induces specific proteins in association with its ability to protect against oxidative stress. Chem Res Toxicol. 2003;16:312-9 pubmed
    ..The present studies provide insights into potential downstream targets of PG signaling. ..
  7. Guo Y, Kotova E, Chen Z, Lee K, Hopper Borge E, Belinsky M, et al. MRP8, ATP-binding cassette C11 (ABCC11), is a cyclic nucleotide efflux pump and a resistance factor for fluoropyrimidines 2',3'-dideoxycytidine and 9'-(2'-phosphonylmethoxyethyl)adenine. J Biol Chem. 2003;278:29509-14 pubmed
    ..We conclude that MRP8 is an amphipathic anion transporter that is able to efflux cAMP and cGMP and to function as a resistance factor for commonly employed purine and pyrimidine nucleotide analogs...
  8. Lechner J, Malloth N, Seppi T, Beer B, Jennings P, Pfaller W. IFN-alpha induces barrier destabilization and apoptosis in renal proximal tubular epithelium. Am J Physiol Cell Physiol. 2008;294:C153-60 pubmed
    ..Caspase-dependent and -independent apoptotic mechanisms are involved. These findings reveal a novel aspect of IFN-alpha actions with implications for normal renal function in immune reactions and during IFN-alpha therapy. ..
  9. Takara K, Tsujimoto M, Kokufu M, Ohnishi N, Yokoyama T. Up-regulation of MDR1 function and expression by cisplatin in LLC-PK1 cells. Biol Pharm Bull. 2003;26:205-9 pubmed
    ..The expression of MDR1 mRNA was increased by the existence of cisplatin for 48 h. These observations taken together suggested that the transient exposure to cisplatin could cause the up-regulation of MDR1 in LLC-PK1 cells. ..
  10. Rustom R, Wang B, McArdle F, Shalamanova L, Alexander J, McArdle A, et al. Oxidative stress in a novel model of chronic acidosis in LLC-PK1 cells. Nephron Exp Nephrol. 2003;95:e13-23 pubmed
    ..The expression of constitutively expressed heat stress protein (HSP) HSC70 and HSP60 increased at pH 7.0. ..
  11. Wangila G, Nagothu K, Steward R, Bhatt R, Iyere P, Willingham W, et al. Prevention of cisplatin-induced kidney epithelial cell apoptosis with a Cu superoxide dismutase-mimetic [copper2II(3,5-ditertiarybutylsalicylate)4(ethanol)4]. Toxicol In Vitro. 2006;20:1300-12 pubmed
    ..Prior treatment of patients who are to undergo treatment with Pt(II)(NH(3))(2)(Cl)(2) for their neoplastic disease with Cu(2)(II)(3,5-DTBS)(4)(Eth)(4) may be beneficial to these patients. ..
  12. Yano T, Itoh Y, Kawamura E, Maeda A, Egashira N, Nishida M, et al. Amphotericin B-induced renal tubular cell injury is mediated by Na+ Influx through ion-permeable pores and subsequent activation of mitogen-activated protein kinases and elevation of intracellular Ca2+ concentration. Antimicrob Agents Chemother. 2009;53:1420-6 pubmed publisher
  13. Lee D, Kang K, Lee H, Kim K. Chemical Characterization of a Renoprotective Metabolite from Termite-Associated Streptomyces sp. RB1 against Cisplatin-Induced Cytotoxicity. Int J Mol Sci. 2018;19: pubmed publisher
  14. Kohda Y, Matsunaga Y, Yonogi K, Kawai Y, Awaya A, Gemba M. Protective effect of serum thymic factor, FTS, on cephaloridine-induced nephrotoxicity in rats. Biol Pharm Bull. 2005;28:2087-91 pubmed
  15. Enokizono J, Kusuhara H, Ose A, Schinkel A, Sugiyama Y. Quantitative investigation of the role of breast cancer resistance protein (Bcrp/Abcg2) in limiting brain and testis penetration of xenobiotic compounds. Drug Metab Dispos. 2008;36:995-1002 pubmed publisher
    ..These results suggest that BCRP limits the tissue penetration of xenobiotic compounds in the blood-brain and -testis barriers, but its in vivo importance is also modulated by P-glycoprotein activity. ..
  16. Gava E, Samad Zadeh A, Zimpelmann J, Bahramifarid N, Kitten G, Santos R, et al. Angiotensin-(1-7) activates a tyrosine phosphatase and inhibits glucose-induced signalling in proximal tubular cells. Nephrol Dial Transplant. 2009;24:1766-73 pubmed publisher
    ..Ang-(1-7) selectively inhibits glucose-stimulated protein synthesis and TGF-beta1. In diabetic nephropathy, Ang-(1-7) may partly counteract the profibrotic effects of high glucose. ..
  17. Pan L, Zeng K, Wang X, Bi H, Hu H, Huang M, et al. Neochamaejasmin B increases the bioavailability of chamaechromone coexisting in Stellera chamaejasme L. via inhibition of MRP2 and BCRP. Int J Pharm. 2015;496:440-7 pubmed publisher
    ..9% and 81.9%, respectively. The mechanism of improving the oral bioavailability of chamaechromone was attributable to the inhibition of the BCRP and MRP2-mediated efflux of chamaechromone by NCB. ..
  18. Ahlenstiel T, Burkhardt G, Kohler H, Kuhlmann M. Bioflavonoids attenuate renal proximal tubular cell injury during cold preservation in Euro-Collins and University of Wisconsin solutions. Kidney Int. 2003;63:554-63 pubmed
    ..Renoprotective effects of bioflavonoids are defined by structure, suggesting that flavonoids are incorporated into membrane lipid bilayers and interfere with membrane lipid peroxidation. ..
  19. Andreucci M, Michael A, Kramers C, Park K, Chen A, Matthaeus T, et al. Renal ischemia/reperfusion and ATP depletion/repletion in LLC-PK(1) cells result in phosphorylation of FKHR and FKHRL1. Kidney Int. 2003;64:1189-98 pubmed
    ..We conclude that PI 3-kinase and Akt are activated after renal ischemia/reperfusion and that Akt phosphorylation leads to phosphorylation of FKHR and FKHRL1, which may affect epithelial cell fate in acute renal failure. ..
  20. Woodahl E, Yang Z, Bui T, Shen D, Ho R. Multidrug resistance gene G1199A polymorphism alters efflux transport activity of P-glycoprotein. J Pharmacol Exp Ther. 2004;310:1199-207 pubmed
  21. Bandler P, Westlake C, Grant C, Cole S, Deeley R. Identification of regions required for apical membrane localization of human multidrug resistance protein 2. Mol Pharmacol. 2008;74:9-19 pubmed publisher
    ..Our results indicate that CL3 is important for interaction with both the glutathione and glucuronide conjugates tested, but that different regions may be involved. ..
  22. Chang K, Sosnovtsev S, Belliot G, Kim Y, Saif L, Green K. Bile acids are essential for porcine enteric calicivirus replication in association with down-regulation of signal transducer and activator of transcription 1. Proc Natl Acad Sci U S A. 2004;101:8733-8 pubmed
  23. Pazos P, Boveri M, Gennari A, Casado J, Fernandez F, Prieto P. Culturing cells without serum: lessons learnt using molecules of plant origin. ALTEX. 2004;21:67-72 pubmed
    ..We found that these reagents of plant origin could be promising alternatives to serum. However, more work and effort is needed to improve cell adaptation, cell attachment, growth rates as well as freezing/thawing protocols. ..
  24. Piao X, Piao X, Kim S, Park J, Kim H, Cai S. Identification and characterization of antioxidants from Sophora flavescens. Biol Pharm Bull. 2006;29:1911-5 pubmed
    ..The results of this study suggest that S. flavescens has excellent antioxidative and kidney-protective potential and that flavonoids from S. flavescens, i.e., sophoraflavanone G and kurarinone, are the active constituents...
  25. Thirone A, Speight P, Zulys M, Rotstein O, Szaszi K, Pedersen S, et al. Hyperosmotic stress induces Rho/Rho kinase/LIM kinase-mediated cofilin phosphorylation in tubular cells: key role in the osmotically triggered F-actin response. Am J Physiol Cell Physiol. 2009;296:C463-75 pubmed publisher
  26. Liang H, Arsenault J, Mortensen J, Park F, Johnson C, Nilakantan V. Partial attenuation of cytotoxicity and apoptosis by SOD1 in ischemic renal epithelial cells. Apoptosis. 2009;14:1176-89 pubmed publisher
  27. Yamashita J, Kita S, Iwamoto T, Ogata M, Takaoka M, Tazawa N, et al. Attenuation of ischemia/reperfusion-induced renal injury in mice deficient in Na+/Ca2+ exchanger. J Pharmacol Exp Ther. 2003;304:284-93 pubmed
  28. Imai Y, Asada S, Tsukahara S, Ishikawa E, Tsuruo T, Sugimoto Y. Breast cancer resistance protein exports sulfated estrogens but not free estrogens. Mol Pharmacol. 2003;64:610-8 pubmed
    ..These results suggest that BCRP does not transport either free estrone or 17beta-estradiol but exports sulfate conjugates of these estrogens. ..
  29. Hamano K, Katafuchi T, Kikumoto K, Minamino N. Calcitonin receptor-stimulating peptide-1 regulates ion transport and growth of renal epithelial cell line LLC-PK1. Biochem Biophys Res Commun. 2005;330:75-80 pubmed
    ..These results indicate that CRSP-1 enhances the (22)Na(+) uptake by the specific activation of NHE1. Taken together, CRSP-1 is considered to be a new regulator for the urinary ion excretion and renal epithelial cell growth. ..
  30. Ko S, Luo X, Hager H, Rojek A, Choi J, Licht C, et al. AE4 is a DIDS-sensitive Cl(-)/HCO(-)(3) exchanger in the basolateral membrane of the renal CCD and the SMG duct. Am J Physiol Cell Physiol. 2002;283:C1206-18 pubmed
    ..In the rat and mouse AE4 functions as a Cl(-)/HCO(-)(3) exchanger in the basolateral membrane of alpha-intercalated cells and may participate in HCO(-)(3) absorption. In the rabbit AE4 may contribute to HCO(-)(3) secretion. ..
  31. Cheron M, Petit C, Bolard J, Gaboriau F. Heat-induced reformulation of amphotericin B-deoxycholate favours drug uptake by the macrophage-like cell line J774. J Antimicrob Chemother. 2003;52:904-10 pubmed
  32. Yang C, Kaushal V, Shah S, Kaushal G. Mcl-1 is downregulated in cisplatin-induced apoptosis, and proteasome inhibitors restore Mcl-1 and promote survival in renal tubular epithelial cells. Am J Physiol Renal Physiol. 2007;292:F1710-7 pubmed
  33. Yang C, Kaushal V, Shah S, Kaushal G. Autophagy is associated with apoptosis in cisplatin injury to renal tubular epithelial cells. Am J Physiol Renal Physiol. 2008;294:F777-87 pubmed publisher
    ..Our results demonstrate that induction of autophagy mounts an adaptive response, suppresses cisplatin-induced apoptosis, and prolongs survival of RTEC. ..
  34. Duffield A, FOLSCH H, Mellman I, Caplan M. Sorting of H,K-ATPase beta-subunit in MDCK and LLC-PK cells is independent of mu 1B adaptin expression. Traffic. 2004;5:449-61 pubmed
  35. Massicot F, Dutertre Catella H, Pham Huy C, Liu X, Duc H, Warnet J. In vitro assessment of renal toxicity and inflammatory events of two protein phosphatase inhibitors cantharidin and nor-cantharidin. Basic Clin Pharmacol Toxicol. 2005;96:26-32 pubmed
    ..We provide a better understanding of the various mechanisms of cantharidin side effects, allowing an easier comparison with nor-cantharidin which could be an attractive therapeutic potential in cancer chemotherapy in western countries. ..
  36. Szaszi K, Sirokmány G, Di Ciano Oliveira C, Rotstein O, Kapus A. Depolarization induces Rho-Rho kinase-mediated myosin light chain phosphorylation in kidney tubular cells. Am J Physiol Cell Physiol. 2005;289:C673-85 pubmed
    ..This pathway may be a central mechanism whereby electrogenic transmembrane transport processes control myosin phosphorylation and thereby regulate paracellular transport. ..
  37. Carriere M, Proux O, Milgram S, Thiebault C, Avoscan L, Barre N, et al. Transmission electron microscopic and X-ray absorption fine structure spectroscopic investigation of U repartition and speciation after accumulation in renal cells. J Biol Inorg Chem. 2008;13:655-62 pubmed publisher
    ..U speciation inside cells was directly and clearly identified for the first time. These results elucidate the role of U speciation in terms of its bioavailability and consequent health effects. ..
  38. Ragolia L, Palaia T, Paric E, Maesaka J. Elevated L-PGDS activity contributes to PMA-induced apoptosis concomitant with downregulation of PI3-K. Am J Physiol Cell Physiol. 2003;284:C119-26 pubmed
    ..We conclude that phorbol ester-induced apoptosis is mediated by L-PGDS phosphorylation and activation by PKC and is accompanied by inhibition of the PI3-K/PKB anti-apoptotic signaling pathways. ..
  39. Ishibashi F. Chronic high glucose inhibits albumin reabsorption by lysosomal alkalinization in cultured porcine proximal tubular epithelial cells (LLC-PK1). Diabetes Res Clin Pract. 2006;72:223-30 pubmed
    ..In conclusion, the chronic HG inhibits albumin reabsorption by lysosomal alkalinization in PTECs, probably due to ATP depletion and down-regulation of vacuolar H(+)-ATPase. ..
  40. Davalos M, Konno S, Eshghi M, Choudhury M. Oxidative renal cell injury induced by calcium oxalate crystal and renoprotection with antioxidants: a possible role of oxidative stress in nephrolithiasis. J Endourol. 2010;24:339-45 pubmed publisher
    ..However, NAC effectively averted such severe cytotoxic effects, sustaining the renal cell integrity. Thus, NAC may provide full renoprotection against COM assault, preventing renal cell injury and ultimate stone formation. ..
  41. Yoon H, Ramachandiran S, Chacko M, Monks T, Lau S. Tuberous sclerosis-2 tumor suppressor modulates ERK and B-Raf activity in transformed renal epithelial cells. Am J Physiol Renal Physiol. 2004;286:F417-24 pubmed
    ..The data indicate that loss of tuberin results in the upregulation of the ERK signaling pathway with subsequent increases in new DNA synthesis, and ultimately, tumor formation. ..
  42. Kobayashi M, Otsuka Y, Itagaki S, Hirano T, Iseki K. Inhibitory effects of statins on human monocarboxylate transporter 4. Int J Pharm. 2006;317:19-25 pubmed
    ..5 mM in hMCT4-expressed oocytes. Lipophilic statins significantly inhibited [(14)C] L-lactic acid uptake in a concentration-dependent manner. In contrast, the inhibitory effects of hydrophilic statins were very weak. ..
  43. Pessoa E, Convento M, Silva R, Oliveira A, Borges F, Schor N. Gentamicin-induced preconditioning of proximal tubular LLC-PK1 cells stimulates nitric oxide production but not the synthesis of heat shock protein. Braz J Med Biol Res. 2009;42:614-20 pubmed
    ..We conclude that preconditioning could be a useful tool to prevent the nephrotoxicity induced by gentamicin. ..
  44. Kaletta T, Van der Craen M, Van Geel A, Dewulf N, Bogaert T, Branden M, et al. Towards understanding the polycystins. Nephron Exp Nephrol. 2003;93:e9-17 pubmed
  45. Sandage B, Sabounjian L, Shipley J, Profy A, Lasseter K, Fox L, et al. Predictive power of an in vitro system to assess drug interactions of an antimuscarinic medication: a comparison of in vitro and in vivo drug-drug interaction studies of trospium chloride with digoxin. J Clin Pharmacol. 2006;46:776-84 pubmed
    ..The in vitro system for assessment of a potential interaction of 2 drugs excreted primarily through the kidney was predictive of the pharmacokinetic outcomes obtained from a clinical setting. ..
  46. Yokozawa T, Kim Y, Kim H, Okamoto T, Sei Y. Protective effect of the Chinese prescription Kangen-karyu against high glucose-induced oxidative stress in LLC-PK1 cells. J Ethnopharmacol. 2007;109:113-20 pubmed
    ..These findings indicate that Kangen-karyu is a potential therapeutic agent that will reduce the damage caused by hyperglycemia-induced oxidative stress associated with diabetes. ..
  47. Person M, Lo H, Towndrow K, Jia Z, Monks T, Lau S. Comparative identification of prostanoid inducible proteins by LC-ESI-MS/MS and MALDI-TOF mass spectrometry. Chem Res Toxicol. 2003;16:757-67 pubmed
  48. Chiu W, Wang Y, Tang M, Shen M. Soft substrate induces apoptosis by the disturbance of Ca2+ homeostasis in renal epithelial LLC-PK1 cells. J Cell Physiol. 2007;212:401-10 pubmed
    ..Thus, soft substrate induces apoptosis in LLC-PK1 cells by the disturbance of Ca(2+) homeostasis. ..
  49. Tajima M, Crane J, Verkman A. Aquaporin-4 (AQP4) associations and array dynamics probed by photobleaching and single-molecule analysis of green fluorescent protein-AQP4 chimeras. J Biol Chem. 2010;285:8163-70 pubmed publisher
    ..Our biophysical measurements in live cells thus reveal extensive AQP4 monomer-monomer and tetramer-tetramer interactions. ..
  50. Loomis P, Kelly A, Zheng L, Changyaleket B, Sekerkova G, Mugnaini E, et al. Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells. J Cell Sci. 2006;119:1655-65 pubmed
    ..Espins do not activate the Arp2/3 complex in vitro, and bundle assembly is not indicative of in-vitro nucleation activity. Our results suggest a novel way to build actin bundles at specific sites in cells. ..
  51. Nilakantan V, Liang H, Mortensen J, Taylor E, Johnson C. Variable effects of the mitoK(ATP) channel modulators diazoxide and 5-HD in ATP-depleted renal epithelial cells. Mol Cell Biochem. 2010;335:211-22 pubmed publisher
    ..This study demonstrates that in cells that are sensitive to ATP depletion-recovery, mitoK(ATP) channels protect against ATP depletion-mediated cytotoxicity and apoptosis through Erk- and JNK-dependant mechanisms. ..
  52. Song I, Han Y, Chung S, Shim C. Contribution of ion-pair complexation with bile salts to the transport of organic cations across LLC-PK1 cell monolayers. Pharm Res. 2003;20:597-604 pubmed
    ..To examine the effect of ion-pair complexation with endogenous bile salts on the transport of organic cations (OCs) across LLC-PK1 cell monolayers...
  53. Raychowdhury M, McLaughlin M, Ramos A, Montalbetti N, Bouley R, Ausiello D, et al. Characterization of single channel currents from primary cilia of renal epithelial cells. J Biol Chem. 2005;280:34718-22 pubmed
    ..Ion channel activity in the primary cilium of renal cells may be an important component of its role as a sensory transducer...
  54. Igoucheva O, Alexeev V, Yoon K. Differential cellular responses to exogenous DNA in mammalian cells and its effect on oligonucleotide-directed gene modification. Gene Ther. 2006;13:266-75 pubmed
    ..In addition, the presented data indicate that the presence of exogenous dsDNA triggered a DNA damage response by activation of ATR (ataxia telangiectasia-Rad3-related) but not ATM (ataxia telangiectasia mutated) pathway...
  55. Ji Y, Neverova I, Van Eyk J, Bennett B. Nitration of tyrosine 92 mediates the activation of rat microsomal glutathione s-transferase by peroxynitrite. J Biol Chem. 2006;281:1986-91 pubmed
  56. Kim Y, Kim Y, Oh Y, Back N, Chung S, Chung H, et al. Protective effect of the ethanol extract of the roots of Brassica rapa on cisplatin-induced nephrotoxicity in LLC-PK1 cells and rats. Biol Pharm Bull. 2006;29:2436-41 pubmed
    ..Taken together, our data indicate that the ethanol extract of the roots of Brassica rapa (EBR) has a protective effect against cisplatin-induced nephrotoxicity because it attenuates oxidative stress...
  57. Nakanishi T, Akabane E, Nanami M, Kiyobayashi Y, Moriguchi R, Hasuike Y, et al. Comparison of cytotoxicity of cysteine and homocysteine for renal epithelial cells. Nephron Exp Nephrol. 2005;100:e11-20 pubmed
    ..We compared the direct toxicity of Hcy on renal tubular cells to that of Cys and examined the mechanism of cell toxicity...
  58. Claffey J, Hogan M, Müller Bunz H, Pampillón C, Tacke M. Oxali-titanocene Y: a potent anticancer drug. ChemMedChem. 2008;3:729-31 pubmed publisher
  59. Chae H, Kim I, Jin H, Kim D, Chung S, Shim C. Effect of ion-pair formation with bile salts on the in vitro cellular transport of berberine. Arch Pharm Res. 2008;31:103-10 pubmed
    ..g., molecular weight, lipophilicity and affinity to relevant transporters) and the characteristics of cell strains (e.g., expression and contribution of responsible transporters to the transport)...
  60. Zhang W, Lim L. Effects of spice constituents on P-glycoprotein-mediated transport and CYP3A4-mediated metabolism in vitro. Drug Metab Dispos. 2008;36:1283-90 pubmed publisher
    ..Among the spices that showed inhibitory effect on MDZ metabolism in HLM, only AMD showed a preincubation time-dependent inhibitory effect on MDZ metabolism in HLM, suggesting the AMD as an irreversible CYP3A4 inhibitor...
  61. Tahara K, Kagawa Y, Takaai M, Taguchi M, Hashimoto Y. Directional transcellular transport of bisoprolol in P-glycoprotein-expressed LLC-GA5-COL150 cells, but not in renal epithelial LLC-PK1 Cells. Drug Metab Pharmacokinet. 2008;23:340-6 pubmed
  62. Yao J, Huang T, Fang X, Chi Y, Zhu Y, Wan Y, et al. Disruption of gap junctions attenuates aminoglycoside-elicited renal tubular cell injury. Br J Pharmacol. 2010;160:2055-68 pubmed publisher
    ..Gap junctions play important roles in the regulation of cell phenotype and in determining cell survival after various insults. Here, we investigated the role of gap junctions in aminoglycoside-induced injury to renal tubular cells...
  63. Ishiguro N, Nozawa T, Tsujihata A, Saito A, Kishimoto W, Yokoyama K, et al. Influx and efflux transport of H1-antagonist epinastine across the blood-brain barrier. Drug Metab Dispos. 2004;32:519-24 pubmed
  64. Didziapetris R, Japertas P, Avdeef A, Petrauskas A. Classification analysis of P-glycoprotein substrate specificity. J Drug Target. 2003;11:391-406 pubmed
    ..The definition of class-specific effects entails construction of the knowledge base that can be very useful in ADME profiling of new drugs...
  65. Chen Z, Vetter M, Chang G, Liu S, Che D, Ding Y, et al. Cyclophilin A functions as an endogenous inhibitor for membrane-bound guanylate cyclase-A. Hypertension. 2004;44:963-8 pubmed
  66. Kita S, Katsuragi T, Iwamoto T. Endothelin-1 enhances the activity of Na+/Ca2+ exchanger type 1 in renal epithelial cells. J Cardiovasc Pharmacol. 2004;44 Suppl 1:S239-43 pubmed
    ..These results suggest that endothelin-1 activates NCX1 in renal epithelial cells through the pathway of protein kinase C and the process related to Na-dependent inactivation...
  67. Ahlenstiel T, Burkhardt G, Kohler H, Kuhlmann M. Improved cold preservation of kidney tubular cells by means of adding bioflavonoids to organ preservation solutions. Transplantation. 2006;81:231-9 pubmed
    ..The aim of this study is to examine whether cold storage induced cell injury can be ameliorated by adding flavonoids directly to preservation solutions...
  68. Xin H, Schwab M, Klotz U. Transport studies with 5-aminosalicylate. Eur J Clin Pharmacol. 2006;62:871-5 pubmed
    ..In addition to local metabolism of 5-ASA, it has been assumed that therapeutic mucosal concentrations of 5-ASA depend on transporter-mediated secretion back to the lumen...
  69. Schmid U, Stopper H, Schweda F, Queisser N, Schupp N. Angiotensin II induces DNA damage in the kidney. Cancer Res. 2008;68:9239-46 pubmed publisher
    ..The majority of detected strand breaks was repaired within 1 hour, but double-strand breaks increased and persisted for at least 24 hours...
  70. Li H, Chung S, Shim C. Characterization of the transport of uracil across Caco-2 and LLC-PK1 cell monolayers. Pharm Res. 2002;19:1495-501 pubmed
    ..The purpose of this study was to characterize the transport of uracil, a pyrimidine nucleobase, in Caco-2 and LLC-PK, cells...
  71. Gennari A, Cortese E, Boveri M, Casado J, Prieto P. Sensitive endpoints for evaluating cadmium-induced acute toxicity in LLC-PK1 cells. Toxicology. 2003;183:211-20 pubmed
    ..Therefore, these endpoints should prove very useful in studying heavy metal-induced acute toxicity. Exposure of the cells to higher concentrations of CdCl(2) (50 microM) revealed the initiation of apoptosis, mediated by caspase-3...
  72. Vetter M, Chen Z, Chang G, Che D, Liu S, Chang C. Cyclosporin A disrupts bradykinin signaling through superoxide. Hypertension. 2003;41:1136-42 pubmed
    ..These findings suggest that CsA activates a NADPH oxidase that releases O2- and disrupts the bradykinin/soluble guanylate cyclase pathway, probably by binding with NO to form peroxynitrite (ONOO-)...
  73. Li Q, Sai Y, Kato Y, Muraoka H, Tamai I, Tsuji A. Transporter-mediated renal handling of nafamostat mesilate. J Pharm Sci. 2004;93:262-72 pubmed
    ..Thus, renal accumulation of NM can be explained by the involvement of the basolateral OCTs, though the influence of the apical membrane transporter remains to be clarified...
  74. Sato Y, Terashima M, Kagiwada N, Tun T, Inagaki M, Kakuta T, et al. Evaluation of proliferation and functional differentiation of LLC-PK1 cells on porous polymer membranes for the development of a bioartificial renal tubule device. Tissue Eng. 2005;11:1506-15 pubmed
    ..Polysulfone membrane is more suitable than cellulose acetate membrane for a bioartificial renal tubule system with regard to LLC-PK1 cell proliferation. Extracellular matrix coating of the membrane further improves cell proliferation...
  75. Uhlig S, Wisløff H, Petersen D. Identification of cytotoxic constituents of Narthecium ossifragum using bioassay-guided fractionation. J Agric Food Chem. 2007;55:6018-26 pubmed
    ..ossifragum; second, the findings should induce future investigations into the possible role of saponins in N. ossifragum-related nephrotoxicosis observed in ruminants that graze on this plant...
  76. Naba A, Reverdy C, Louvard D, Arpin M. Spatial recruitment and activation of the Fes kinase by ezrin promotes HGF-induced cell scattering. EMBO J. 2008;27:38-50 pubmed
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    ..P-glycoprotein (P-gp), encoded by MDR1 (or ABCB1), is important in anticancer drug delivery and resistance. We evaluated alterations in P-gp-mediated transport of anticancer agents due to the MDR1 G1199A polymorphism...
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    ..LS did not appear to have an effect on collagen production. In conclusion, our study demonstrates the potential of LS to function as a stimulator of the inflammatory stage of tissue repair, TNF-alpha production, and of angiogenesis...
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    ..We constructed a bioartificial renal tubule device by using renal tubule epithelial cells in an artificial membrane, and evaluated transport properties of the device for 2 weeks...
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    ..Biased MT release may provide a mechanism for MT-dependent positioning of components necessary for specifying the site of contractile ring formation...
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    ..Our findings demonstrate, for the first time, that C(2)H(2) zinc fingers can link DNA binding to HAT signaling and suggest a dynamic role for DNA-binding proteins in the enzymatic control of transcription...
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    ..However, the circulating concentrations of DLS do not acutely inhibit the sodium pump to a degree consistent with the observed changes in proximal tubule sodium reabsorption...
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    ..This would offer an explanation for the observed clinical drug-drug interactions between the antibiotic and cyclosporine...
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    ..Taken together, the new findings suggest that LLC-PK1 cells contain a pool of Src-interacting Na/K-ATPase that not only regulates Src activity but also serves as a receptor for ouabain to activate protein kinases...
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    ..These findings suggest that some natural products containing these terpenoids may inhibit P-gp-mediated transport and interact with P-gp substrates in the intestinal absorption process...
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    ..These studies indicate that glucocorticoids regulate acid-base homeostasis during metabolic acidosis in part by regulating expression of the System N transporter SNAT3...
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    ..These studies have identified a component of the native gentamicin congener mixture that retains its bactericidal properties with minimal or no apparent nephrotoxicity...
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    ..7%, and Sp1-binding and promoter activity were significantly decreased. This is the first study to clarify the transcriptional mechanisms of the MATE1 gene and to identify a SNP affecting the promoter activity of hMATE1...
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    ..Identification of the heteromultimeric TRPP2/TRPC1 channel has implications in mechanosensation and cilium-based Ca(2+) signalling...