3t3 l1 cells


Summary: A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.

Top Publications

  1. Gonzalez E, McGraw T. Insulin-modulated Akt subcellular localization determines Akt isoform-specific signaling. Proc Natl Acad Sci U S A. 2009;106:7004-9 pubmed publisher
    ..Together, our findings reveal the stimulus-induced subcellular compartmentalization of Akt kinases as a mechanism contributing to specify Akt isoform functions...
  2. Shen Y, Kim Y, Yun C, Moon Y, Kim S. Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells. BMB Rep. 2009;42:691-6 pubmed
    ..Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes...
  3. Xiong W, Jordens I, Gonzalez E, McGraw T. GLUT4 is sorted to vesicles whose accumulation beneath and insertion into the plasma membrane are differentially regulated by insulin and selectively affected by insulin resistance. Mol Biol Cell. 2010;21:1375-86 pubmed publisher
    ..Differences in insulin sensitivities of various processes may be a general mechanism for the development of the physiologically important phenomenon of selective insulin resistance. ..
  4. Lin Q, Gao Z, Alarcon R, Ye J, Yun Z. A role of miR-27 in the regulation of adipogenesis. FEBS J. 2009;276:2348-58 pubmed
    ..Our data strongly suggest that miR-27 represents a new class of adipogenic inhibitors and may play a role in the pathological development of obesity...
  5. Stenson B, Ryden M, Steffensen K, Wahlen K, Pettersson A, Jocken J, et al. Activation of liver X receptor regulates substrate oxidation in white adipocytes. Endocrinology. 2009;150:4104-13 pubmed publisher
    ..We propose that LXR has an important role in the regulation of substrate oxidation and the switch between lipids and carbohydrates as cellular fuel in both human and murine white adipocytes...
  6. Lopez J, Burchfield J, Blair D, Mele K, Ng Y, Vallotton P, et al. Identification of a distal GLUT4 trafficking event controlled by actin polymerization. Mol Biol Cell. 2009;20:3918-29 pubmed publisher
    ..These data clearly resolve multiple steps of the final stages of GLUT4 trafficking, demonstrating a crucial role for actin in the final stage of this process. ..
  7. Laurencikiene J, Stenson B, Arvidsson Nordström E, Agustsson T, Langin D, Isaksson B, et al. Evidence for an important role of CIDEA in human cancer cachexia. Cancer Res. 2008;68:9247-54 pubmed publisher
    ..001). We conclude that CIDEA is involved in adipose tissue loss in cancer cachexia and this may, at least in part, be due to its ability to inactivate PDC, thereby switching substrate oxidation in human fat cells from glucose to FAs...
  8. Polak P, Cybulski N, Feige J, Auwerx J, Ruegg M, Hall M. Adipose-specific knockout of raptor results in lean mice with enhanced mitochondrial respiration. Cell Metab. 2008;8:399-410 pubmed publisher
    ..These results suggest that adipose mTORC1 is a regulator of adipose metabolism and, thereby, controls whole-body energy homeostasis...
  9. Tejerina S, De Pauw A, Vankoningsloo S, Houbion A, Renard P, de Longueville F, et al. Mild mitochondrial uncoupling induces 3T3-L1 adipocyte de-differentiation by a PPARgamma-independent mechanism, whereas TNFalpha-induced de-differentiation is PPARgamma dependent. J Cell Sci. 2009;122:145-55 pubmed publisher
    ..These results highlight some new mechanisms that might potentially be involved in adipocyte de-differentiation initiated by a mitochondrial uncoupling...

More Information

Publications107 found, 100 shown here

  1. Kilroy G, Zhang X, Floyd Z. PPAR-gamma AF-2 domain functions as a component of a ubiquitin-dependent degradation signal. Obesity (Silver Spring). 2009;17:665-73 pubmed publisher
    ..These results indicate that the ubiquitin-proteasome pathway is an integral determinant of PPAR-gamma activity, targeting PPAR-gamma for proteasomal degradation via ubiquitin independent and ubiquitin dependent mechanisms. ..
  2. Huq M, Ha S, Barcelona H, Wei L. Lysine methylation of nuclear co-repressor receptor interacting protein 140. J Proteome Res. 2009;8:1156-67 pubmed publisher
    ..This study reveals a potential hierarchy of modifications, at least for lysine and arginine methylation, which bidirectionally regulate the functionality of a nonhistone protein...
  3. Lee M, Rao Y, Chen K, Lee Y, Tzeng Y. Effect of flavonol glycosides from Cinnamomum osmophloeum leaves on adiponectin secretion and phosphorylation of insulin receptor-beta in 3T3-L1 adipocytes. J Ethnopharmacol. 2009;126:79-85 pubmed publisher
    ..Cinnamomum osmophloeum is used for various ethnomedical conditions in Taiwan including diabetic complications...
  4. Sommer G, Kralisch S, Lipfert J, Weise S, Krause K, Jessnitzer B, et al. Amyloid precursor protein expression is induced by tumor necrosis factor alpha in 3T3-L1 adipocytes. J Cell Biochem. 2009;108:1418-22 pubmed publisher
    ..Taken together, we show for the first time a potent upregulation of APP by TNFalpha suggesting a potential role of this adipocyte-secreted protein in TNFalpha-induced insulin resistance and inflammatory disease. ..
  5. Otway D, Frost G, Johnston J. Circadian rhythmicity in murine pre-adipocyte and adipocyte cells. Chronobiol Int. 2009;26:1340-54 pubmed publisher
    ..Moreover, secretion of leptin may also be regulated by the adipocyte clock. ..
  6. Jun D, Na K, Kim W, Kwak D, Kwon E, Yoon J, et al. Melanocortins induce interleukin 6 gene expression and secretion through melanocortin receptors 2 and 5 in 3T3-L1 adipocytes. J Mol Endocrinol. 2010;44:225-36 pubmed publisher
    ..These results suggest that alpha-MSH in addition to ACTH may function as a regulator of inflammation by regulating cytokine production. ..
  7. Jack B, Crossley M. GATA proteins work together with friend of GATA (FOG) and C-terminal binding protein (CTBP) co-regulators to control adipogenesis. J Biol Chem. 2010;285:32405-14 pubmed publisher
    ..These results implicate FOGs and CTBPs as partners of GATA proteins in the control of adipocyte proliferation and differentiation. ..
  8. Han Y, Choi Y, Lee S, Jin Y, Cheong H, Lee K. Yin Yang 1 is a multi-functional regulator of adipocyte differentiation in 3T3-L1 cells. Mol Cell Endocrinol. 2015;413:217-27 pubmed publisher
    ..These results indicate that YY1 repressed adipocyte differentiation by repressing the activity of adipogenic transcriptional factors in 3T3-L1 cells. ..
  9. Puhl A, Milton F, Cvoro A, Sieglaff D, Campos J, Bernardes A, et al. Mechanisms of peroxisome proliferator activated receptor γ regulation by non-steroidal anti-inflammatory drugs. Nucl Recept Signal. 2015;13:e004 pubmed publisher
    ..We propose that it is important to consider how individual NSAIDs interact with PPARγ to understand their activities, and that it will be interesting to determine whether high dose NSAID therapies result in PPAR activation. ..
  10. Nakabayashi H, Hashimoto T, Ashida H, Nishiumi S, Kanazawa K. Inhibitory effects of caffeine and its metabolites on intracellular lipid accumulation in murine 3T3-L1 adipocytes. Biofactors. 2008;34:293-302 pubmed publisher
  11. Zhang T, Guan H, Yang K. Keratinocyte growth factor promotes preadipocyte proliferation via an autocrine mechanism. J Cell Biochem. 2010;109:737-46 pubmed publisher
    ..Aberrant KGF expression may have consequences not only for normal adipose tissue growth but also for the pathogenesis of obesity. ..
  12. Kurokawa J, Arai S, Nakashima K, Nagano H, Nishijima A, Miyata K, et al. Macrophage-derived AIM is endocytosed into adipocytes and decreases lipid droplets via inhibition of fatty acid synthase activity. Cell Metab. 2010;11:479-92 pubmed publisher
    ..Interestingly, metabolic rates are comparable in AIM(-)(/-) and AIM(+/+) mice, suggesting that AIM specifically influences adipocyte status. Thus, this AIM function in adipocytes may be physiologically relevant to obesity progression. ..
  13. Yoshioka H, Mizuno Y, Yamaguchi T, Ichimaru Y, Takeya K, Hitotsuyanagi Y, et al. Methyl dehydroabietate counters high fat diet-induced insulin resistance and hepatic steatosis by modulating peroxisome proliferator-activated receptor signaling in mice. Biomed Pharmacother. 2018;99:214-219 pubmed publisher
    ..Our findings demonstrate that mDA reduces obesity-induced glucose and insulin tolerance by inducing PPAR expression. ..
  14. Peterfy M, Harris T, Fujita N, Reue K. Insulin-stimulated interaction with 14-3-3 promotes cytoplasmic localization of lipin-1 in adipocytes. J Biol Chem. 2010;285:3857-64 pubmed publisher
    ..In summary, our studies suggest that insulin may modulate the cellular function of lipin-1 by regulating its subcellular localization through interactions with 14-3-3 proteins. ..
  15. Ji Z, Mei F, Cheng X. Epac, not PKA catalytic subunit, is required for 3T3-L1 preadipocyte differentiation. Front Biosci (Elite Ed). 2010;2:392-8 pubmed
    ..Our study demonstrated that while PKA catalytic activity is dispensable, activation of Epac is necessary but not sufficient for adipogenic conversion of 3T3-L1 cells. ..
  16. Moreno Navarrete J, Escoté X, Ortega F, Camps M, Ricart W, Zorzano A, et al. Lipopolysaccharide binding protein is an adipokine involved in the resilience of the mouse adipocyte to inflammation. Diabetologia. 2015;58:2424-34 pubmed publisher
    ..In summary, LBP is a proinflammatory soluble adipokine that acts as a brake for adipogenesis, strengthening the negative effects of palmitate and LPS on adipocyte differentiation. ..
  17. Huang W, Chen C, Wu S. Almond Skin Polyphenol Extract Inhibits Inflammation and Promotes Lipolysis in Differentiated 3T3-L1 Adipocytes. J Med Food. 2017;20:103-109 pubmed publisher
    ..This study suggests that ASP regulates lipolysis through activation of AMPK, reduced adipogenesis, and suppresses proinflammatory cytokines in adipocytes. ..
  18. Le T, Cheng J. Single-cell profiling reveals the origin of phenotypic variability in adipogenesis. PLoS ONE. 2009;4:e5189 pubmed publisher
    ..Single-cell profiling reveals the kinetics of an insulin signaling cascade as the origin of phenotypic variability in drug-inducible adipogenesis. ..
  19. Wueest S, Rapold R, Schoenle E, Konrad D. Fas activation in adipocytes impairs insulin-stimulated glucose uptake by reducing Akt. FEBS Lett. 2010;584:4187-92 pubmed publisher
    ..In conclusion, Fas activation in adipocytes decreases Akt expression and thereby impairs insulin sensitivity. ..
  20. Lee J, Choi J, Lee W, Ko K, Kim S. Dehydrodiconiferyl alcohol (DHCA) modulates the differentiation of Th17 and Th1 cells and suppresses experimental autoimmune encephalomyelitis. Mol Immunol. 2015;68:434-44 pubmed publisher
    ..Taken together, our data indicate that DHCA may be a potential candidate as an agent for the control of Th17 and Th1-mediated inflammatory diseases. ..
  21. Oon S, Nallappan M, Kassim N, Shohaimi S, Sa ariwijaya M, Tee T, et al. Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC. Biochem Biophys Res Commun. 2016;478:1403-8 pubmed publisher
    ..To conclude, XNT could be a potential hypolipidemic agent to improve cardiovascular health in the future. ..
  22. Kobayashi H, Matsuda M, Fukuhara A, Komuro R, Shimomura I. Dysregulated glutathione metabolism links to impaired insulin action in adipocytes. Am J Physiol Endocrinol Metab. 2009;296:E1326-34 pubmed publisher
    ..These results suggest that decreased GPX activity and increased gamma-GCS expression lead to overaccumulation of GSH, which might be involved in the pathogenesis of insulin resistance in obesity. ..
  23. Almendro V, Fuster G, Ametller E, Costelli P, Pilla F, Busquets S, et al. Interleukin-15 increases calcineurin expression in 3T3-L1 cells: possible involvement on in vivo adipocyte differentiation. Int J Mol Med. 2009;24:453-8 pubmed
    ..These data suggest that IL-15 directly inhibits adipogenesis, possibly by upregulating alpha-calcineurin and preventing the induction of adipocyte differentiation. ..
  24. Chow Y, Iwata Y, Sato F. Dihydrosanguinarine Enhances Glucose Uptake in Mouse 3T3-L1 Cells. ACS Omega. 2017;2:6916-6925 pubmed publisher
    ..DHS significantly enhanced the glucose uptake in 3T3-L1 adipocytes without observable cytotoxicity at the effective concentration (5 ?M) applied...
  25. Vernochet C, Peres S, Davis K, McDonald M, Qiang L, Wang H, et al. C/EBPalpha and the corepressors CtBP1 and CtBP2 regulate repression of select visceral white adipose genes during induction of the brown phenotype in white adipocytes by peroxisome proliferator-activated receptor gamma agonists. Mol Cell Biol. 2009;29:4714-28 pubmed publisher
    ..Developing strategies to enhance the brown phenotype in white adipocytes while reducing secretion of stress-related cytokines from visceral WAT is a means to combat obesity-associated disorders. ..
  26. Frizzell N, Rajesh M, Jepson M, Nagai R, Carson J, Thorpe S, et al. Succination of thiol groups in adipose tissue proteins in diabetes: succination inhibits polymerization and secretion of adiponectin. J Biol Chem. 2009;284:25772-81 pubmed publisher
  27. Wang Y, Zhang J, Chen Y, Jiang L, Ji W, Xu T. Characterization of GLUT4-containing vesicles in 3T3-L1 adipocytes by total internal reflection fluorescence microscopy. Sci China C Life Sci. 2009;52:665-71 pubmed publisher
    ..These data demonstrate that vertical GLUT4-containing vesicles correspond to specialized secretory GSVs, which approach the PM directly and bypass the constitutive recycling pathway. ..
  28. Kim J, Xiao H, Tan Y, Wang Z, Paul Seale J, Qu X. The effects and mechanism of saponins of Panax notoginseng on glucose metabolism in 3T3-L1 cells. Am J Chin Med. 2009;37:1179-89 pubmed
    ..The results of this study indicate that SPN may have a therapeutic potential for hyperglycaemia in type 2 diabetes. ..
  29. Veilleux A, Houde V, Bellmann K, Marette A. Chronic inhibition of the mTORC1/S6K1 pathway increases insulin-induced PI3K activity but inhibits Akt2 and glucose transport stimulation in 3T3-L1 adipocytes. Mol Endocrinol. 2010;24:766-78 pubmed publisher
    ..However, this study also shows that chronic inhibition of the mTORC1/S6K1 pathway uncouples IRS-1/PI3K signaling from insulin-induced glucose transport due to impaired activation of Akt2 and blunted glucose transporter 4 expression. ..
  30. Espina A, Méndez Vidal C, Moreno Mateos M, Saez C, Romero Franco A, Japon M, et al. Induction of Dlk1 by PTTG1 inhibits adipocyte differentiation and correlates with malignant transformation. Mol Biol Cell. 2009;20:3353-62 pubmed publisher
  31. Whelan S, Dias W, Thiruneelakantapillai L, Lane M, Hart G. Regulation of insulin receptor substrate 1 (IRS-1)/AKT kinase-mediated insulin signaling by O-Linked beta-N-acetylglucosamine in 3T3-L1 adipocytes. J Biol Chem. 2010;285:5204-11 pubmed publisher
    ..We conclude that one of the steps at which O-GlcNAc contributes to insulin resistance is by inhibiting phosphorylation at the Y(608)XXM PI3K p85 binding motif in IRS-1 and possibly at PDK1 as well. ..
  32. Blouin C, Prado C, Takane K, Lasnier F, Garcia Ocana A, Ferre P, et al. Plasma membrane subdomain compartmentalization contributes to distinct mechanisms of ceramide action on insulin signaling. Diabetes. 2010;59:600-10 pubmed publisher
    ..Our results show that ceramide can switch from a PKCzeta-dependent mechanism to a PP2A pathway, acting negatively on PKB/Akt, and hence revealing a critical role of CEMs of the PM in this process. ..
  33. Fujimori K, Ueno T, Nagata N, Kashiwagi K, Aritake K, Amano F, et al. Suppression of adipocyte differentiation by aldo-keto reductase 1B3 acting as prostaglandin F2alpha synthase. J Biol Chem. 2010;285:8880-6 pubmed publisher
    ..These results indicate that AKR1B3 acts as the PGFS in adipocytes and that AKR1B3-produced PGF(2alpha) suppressed adipocyte differentiation by acting through FP receptors. ..
  34. Eom S, Liu S, Su M, Noh T, Hong J, Kim N, et al. Synthesis of Phthalimide Derivatives as Potential PPAR-? Ligands. Mar Drugs. 2016;14: pubmed publisher
    ..However, in in vitro assays, PD1 and PD2 consistently displayed significant PPAR-? activation in Ac2F and HepG2 cells, and adipogenic activity in 3T3-L1 preadipocytes. ..
  35. Weiszenstein M, Musutova M, Plihalova A, Westlake K, Elkalaf M, Koc M, et al. Adipogenesis, lipogenesis and lipolysis is stimulated by mild but not severe hypoxia in 3T3-L1 cells. Biochem Biophys Res Commun. 2016;478:727-32 pubmed publisher
    ..Mild, but not severe, hypoxia stimulates lipolysis and promotes adipocyte differentiation, probably through excess of acetyl-CoA originating from tricarboxylic acid cycle independently of HIF activation. ..
  36. Peng Y, Li H, Li X, Yu S, Xiang H, Peng J, et al. MicroRNA-215 impairs adipocyte differentiation and co-represses FNDC3B and CTNNBIP1. Int J Biochem Cell Biol. 2016;79:104-112 pubmed publisher
    ..Further studies in mouse 3T3-L1 cell-line suggests that miR-215-5p is a negative regulator of adipocyte differentiation through post-transcriptional regulation of FNDC3B and CTNNBIP1 during early adipogenesis. ..
  37. Wang J, Wu Y, Guo J, Fei X, Yu L, Ma S. Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells. Oncotarget. 2017;8:81880-81891 pubmed publisher
    ..Therefore, our results reveal a novel mechanism in the adipocyte-derived exosome-mediated promotion of lung tumor metastasis, which extends our knowledge regarding obesity and tumor progression...
  38. Dreijerink K, Varier R, Van Beekum O, Jeninga E, Höppener J, Lips C, et al. The multiple endocrine neoplasia type 1 (MEN1) tumor suppressor regulates peroxisome proliferator-activated receptor gamma-dependent adipocyte differentiation. Mol Cell Biol. 2009;29:5060-9 pubmed publisher
    ..We propose that menin is an important factor in PPARgamma-mediated adipogenesis and that loss of PPARgamma function may contribute to lipoma development in MEN1 patients. ..
  39. Gracia A, Miranda J, Fernández Quintela A, Eseberri I, Garcia Lacarte M, Milagro F, et al. Involvement of miR-539-5p in the inhibition of de novo lipogenesis induced by resveratrol in white adipose tissue. Food Funct. 2016;7:1680-8 pubmed publisher
    ..In conclusion, the up-regulation of miR-539-5p is involved in the inhibition of de novo lipogenesis induced by resveratrol in WAT. ..
  40. Haj Yasein N, Berg O, Jernerén F, Refsum H, Nebb H, Dalen K. Cysteine deprivation prevents induction of peroxisome proliferator-activated receptor gamma-2 and adipose differentiation of 3T3-L1 cells. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862:623-635 pubmed publisher
    ..Our results provide further evidence for the hypothesis that plasma cysteine is a casual determinant for body fat mass. ..
  41. Lv Q, Wang Y, Wang W, Wang L, Zhou X. Effect of pioglitazone on visfatin expression in 3T3-L1 adipocytes and SD rats. Endocr Res. 2009;34:130-41 pubmed publisher
  42. Sharma N, Nagrath D, Yarmush M. Adipocyte-derived basement membrane extract with biological activity: applications in hepatocyte functional augmentation in vitro. FASEB J. 2010;24:2364-74 pubmed publisher
    ..The demonstrated synthesis, isolation, characterization, and application of Adipogel provide immense potential for tissue engineering and regenerative medicine applications. ..
  43. Wakai E, Aritake K, Urade Y, Fujimori K. Prostaglandin D2 enhances lipid accumulation through suppression of lipolysis via DP2 (CRTH2) receptors in adipocytes. Biochem Biophys Res Commun. 2017;490:393-399 pubmed publisher
    ..These results indicate that PGD2 suppressed the lipolysis by repression of the cAMP-PKA-HSL axis through DP2R in adipocytes. ..
  44. Kang J, Park J, Kim H, Jung Y, Youn D, Lim S, et al. Secoisolariciresinol diglucoside inhibits adipogenesis through the AMPK pathway. Eur J Pharmacol. 2018;820:235-244 pubmed publisher
    ..These results suggest that SDG inhibits adipogenesis by activating AMPK?, suggesting it could be an attractive therapeutic candidate for the treatment of obesity. ..
  45. Fang P, Yu M, Min W, Han S, Shi M, Zhang Z, et al. Beneficial effect of baicalin on insulin sensitivity in adipocytes of diet-induced obese mice. Diabetes Res Clin Pract. 2018;139:262-271 pubmed publisher
    ..Baicalin plays a significant role in elevation of glucose uptake and insulin sensitivity to promote glucose clearance. ..
  46. Pi J, Leung L, Xue P, Wang W, Hou Y, Liu D, et al. Deficiency in the nuclear factor E2-related factor-2 transcription factor results in impaired adipogenesis and protects against diet-induced obesity. J Biol Chem. 2010;285:9292-300 pubmed publisher
  47. Sung H, Jun J, Kang S, Kim H, Shin D, Kang I, et al. Novel Danshen methoxybenzo[b]furan derivative antagonizing adipogenic differentiation and production of inflammatory adipokines. Chem Biol Interact. 2010;188:457-66 pubmed publisher
  48. Lobo S, Wiczer B, Bernlohr D. Functional analysis of long-chain acyl-CoA synthetase 1 in 3T3-L1 adipocytes. J Biol Chem. 2009;284:18347-56 pubmed publisher
    ..These findings identify a primary role of ACSL1 in adipocytes not in control of lipid influx, as previously considered, but in lipid efflux and fatty acid-induced insulin resistance...
  49. Goebel M, Staels B, Unger T, Kintscher U, Gust R. Characterization of new PPARgamma agonists: benzimidazole derivatives - the importance of position 2. ChemMedChem. 2009;4:1136-42 pubmed publisher
    ..4 microM) was abolished by an additional hydrophobic 4-chloro substituent (4 h; EC(50)=0.55 microM) or an elongation of the alkyl chain between the phenyl and the benzimidazole core (4 g; EC(50)=0.31 microM)...
  50. Hatanaka M, Shimba S, Sakaue M, Kondo Y, Kagechika H, Kokame K, et al. Hypoxia-inducible factor-3alpha functions as an accelerator of 3T3-L1 adipose differentiation. Biol Pharm Bull. 2009;32:1166-72 pubmed
    ..HIF-3alpha, which is induced by HIF-2alpha, might function as an accelerator of adipogenesis...
  51. Moise A, Lobo G, Erokwu B, Wilson D, Peck D, Alvarez S, et al. Increased adiposity in the retinol saturase-knockout mouse. FASEB J. 2010;24:1261-70 pubmed publisher
    ..Moise, A. R., Lobo, G. P., Erokwu, B., Wilson, D. L., Peck, D., Alvarez, S., Domínguez, M., Alvarez, R., Flask, C. A., de Lera, A. R., von Lintig, J., Palczewski, K. Increased adiposity in the retinol saturase-knockout mouse...
  52. Pettersson A, Laurencikiene J, Mejhert N, Naslund E, Bouloumie A, Dahlman I, et al. A possible inflammatory role of twist1 in human white adipocytes. Diabetes. 2010;59:564-71 pubmed publisher
    ..Twist1 is a transcription factor that is highly expressed in murine brown and white adipose tissue (WAT) and negatively regulates fatty acid oxidation in mice. The role of twist1 in WAT is not known and was therefore examined...
  53. Ye F, Than A, Zhao Y, Goh K, Chen P. Vesicular storage, vesicle trafficking, and secretion of leptin and resistin: the similarities, differences, and interplays. J Endocrinol. 2010;206:27-36 pubmed publisher
    ..Interestingly, these two adipokines adversely influence each other on secretion and vesicle trafficking. Finally, we demonstrated that both leptin and resistin secretion are Ca(2+) dependent...
  54. Poulos S, Dodson M, Hausman G. Cell line models for differentiation: preadipocytes and adipocytes. Exp Biol Med (Maywood). 2010;235:1185-93 pubmed publisher
    ..The focus of this brief review is to highlight similarities and differences in adipocyte models to aid in appropriate model selection and data interpretation for successful advancement of our understanding of adipocyte biology...
  55. Higashi K, Mikami T, Yamada T, Kawashima H, Kimura T, Kaneko H. A novel adipokine GM2AP impairs insulin signaling. Biochem Biophys Res Commun. 2010;402:571-6 pubmed publisher
    ..Furthermore, treatment of rat pheochromocytoma PC12 NS1 cells with GM2AP impaired NGF signal transduction. Taken together, these results provide novel insights into the physiological functions of GM2AP in obesity...
  56. Kim J, Han D, Kim J, Lee S, Kim S, Woo J, et al. PPAR gamma partial agonist, KR-62776, inhibits adipocyte differentiation via activation of ERK. Cell Mol Life Sci. 2009;66:1766-81 pubmed publisher
    ..These results support the conclusion that the localization of PPAR gamma is one of the key factors explaining the biological responses of the ligands...
  57. Yang J, Yuan C, Wei L, Yi F, Song F. Melanin-concentrating hormone receptor 2 affects 3T3-L1 preadipocyte differentiation. Mol Cell Endocrinol. 2009;311:11-7 pubmed publisher
    ..These findings suggest that MCHR2 positively mediates the regulation of MCH during preadipocyte differentiation and is involved in energy balance regulation without affecting preadipocyte proliferation...
  58. Takahashi Shiga N, Utsunomiya H, Miki Y, Nagase S, Kobayashi R, Matsumoto M, et al. Local biosynthesis of estrogen in human endometrial carcinoma through tumor-stromal cell interactions. Clin Cancer Res. 2009;15:6028-34 pubmed publisher
    ..However, the effect of tumor-stromal interactions on local estrogen biosynthesis in endometrial carcinomas remains largely unknown...
  59. Yea K, Kim J, Yoon J, Kwon T, Kim J, Lee B, et al. Lysophosphatidylcholine activates adipocyte glucose uptake and lowers blood glucose levels in murine models of diabetes. J Biol Chem. 2009;284:33833-40 pubmed publisher
    ..Furthermore, LPC improved blood glucose levels in mouse models of type 1 and 2 diabetes. These results suggest that an understanding of the mode of action of LPC may provide a new perspective of glucose homeostasis...
  60. Gagnon A, Antunes T, Ly T, Pongsuwan P, Gavin C, Lochnan H, et al. Thyroid-stimulating hormone stimulates lipolysis in adipocytes in culture and raises serum free fatty acid levels in vivo. Metabolism. 2010;59:547-53 pubmed publisher
    ..In summary, TSH stimulates lipolysis and phosphorylation of perilipin and HSL in a protein kinase A-dependent manner in differentiated adipocytes in culture and raises serum FFA levels in vivo...
  61. Min L, Yanase T, Tanaka T, Fan W, Nomura M, Kawate H, et al. A novel synthetic androgen receptor ligand, S42, works as a selective androgen receptor modulator and possesses metabolic effects with little impact on the prostate. Endocrinology. 2009;150:5606-16 pubmed publisher
    ..Taken together, S42 works as an AR agonist in muscle and as an AR antagonist in the prostate, pituitary gland, and liver, accompanying beneficial potentials on lipid metabolism...
  62. Yu B, Cook C, Santanam N. The aporphine alkaloid boldine induces adiponectin expression and regulation in 3T3-L1 cells. J Med Food. 2009;12:1074-83 pubmed publisher
    ..Our results indicate that boldine is able to modulate the expression of adiponectin and its regulators in 3T3-L1 cells and has the potential to be beneficial in obesity-related cardiovascular disease...
  63. Huo Y, Guo X, Li H, Wang H, Zhang W, Wang Y, et al. Disruption of inducible 6-phosphofructo-2-kinase ameliorates diet-induced adiposity but exacerbates systemic insulin resistance and adipose tissue inflammatory response. J Biol Chem. 2010;285:3713-21 pubmed publisher
    ..Together, these data suggest that PFKFB3/iPFK2, although contributing to adiposity, protects against diet-induced insulin resistance and adipose tissue inflammatory response...
  64. Song S, Hong Y, Sasaki S, Roh S, Katoh K. Prostatic androgen-repressed message-1 as a regulator of adipocyte differentiation in the mouse. Tohoku J Exp Med. 2009;219:311-7 pubmed
    ..These results indicate that mPARM-1 might be involved in the regulation of fat accumulation and adipocyte differentiation...
  65. Vila Bedmar R, Lorenzo M, Fernández Veledo S. Adenosine 5'-monophosphate-activated protein kinase-mammalian target of rapamycin cross talk regulates brown adipocyte differentiation. Endocrinology. 2010;151:980-92 pubmed publisher
    ..Promotion of BAT development in WAT by pharmacological activation of AMPK may have potential in treating obesity by acting on energy dissipation...
  66. Carter E, Rayner B, McLeod A, Wu L, Marshall C, Levina A, et al. Silicon nitride as a versatile growth substrate for microspectroscopic imaging and mapping of individual cells. Mol Biosyst. 2010;6:1316-22 pubmed publisher
    ..The cells adhered strongly to Si(3)N(4) membranes and visually displayed normal proliferative and phenotypic growth; more importantly, rapid alcohol fixation of cells did not affect their structural integrity for subsequent analyses...
  67. Yao Y, Li X, Zhao W, Zeng Y, Shen H, Xiang H, et al. Anti-obesity effect of an isoflavone fatty acid ester on obese mice induced by high fat diet and its potential mechanism. Lipids Health Dis. 2010;9:49 pubmed publisher
    ..In order to investigate the anti-obesity effect of compound 1a and its potential mechanism of influence in adipocyte differentiation, Obese male C57BL/6J mice induced by high-fat diet (HFD) and rat preadipocytes (3T3-L1 cell) were used...
  68. Chen H, Dardik B, Qiu L, Ren X, Caplan S, Burkey B, et al. Cevoglitazar, a novel peroxisome proliferator-activated receptor-alpha/gamma dual agonist, potently reduces food intake and body weight in obese mice and cynomolgus monkeys. Endocrinology. 2010;151:3115-24 pubmed publisher
  69. Mulumba M, Jossart C, Granata R, Gallo D, Escher E, Ghigo E, et al. GPR103b functions in the peripheral regulation of adipogenesis. Mol Endocrinol. 2010;24:1615-25 pubmed publisher
    ..Furthermore, QRFP was bioactive in omental adipocytes from obese individuals, inhibiting ISO-induced lipolysis in these cells. Our results suggest that GPR103b and QRFP work in an autocrine/paracrine manner to regulate adipogenesis...
  70. Campbell J, Fediuc S, Hawke T, Riddell M. Endurance exercise training increases adipose tissue glucocorticoid exposure: adaptations that facilitate lipolysis. Metabolism. 2009;58:651-60 pubmed publisher
    ..In vitro, GCs are capable of increasing lipolysis, but depend upon the presence of GR. We propose that GCs play a significant role in changing the phenotype of adipose tissue during exercise training, resulting in decreased fat mass...
  71. Jing K, Heo J, Song K, Seo K, Park J, Kim J, et al. Expression regulation and function of Pref-1 during adipogenesis of human mesenchymal stem cells (MSCs). Biochim Biophys Acta. 2009;1791:816-26 pubmed publisher
    ..Taken together, our study demonstrates that IBMX accelerates accumulation of lipid in MSCs only under the circumstance that the negative effect of Pref-1 induced by IBMX on the adipogenesis is overcome by dexamethasone...
  72. Qiu J, Zhang M, Zhou X, Cheng R, Cao X, Wang B, et al. [Construction of eukaryotic expression vector of LYRM1, a novel gene related with human obesity, and establishment of its stable transfected 3T3-L1 cell line]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009;38:493-7 pubmed
    ..To construct the eukaryotic expression vector of LYRM1 and to transfect it to preadipocytes cell line 3T3-L1...
  73. Minami R, Yamamoto M, Takahama S, Ando H, Miyamura T, Suematsu E. High molecular weight form of adiponectin in antiretroviral drug-induced dyslipidemia in HIV-infected Japanese individuals based on in vivo and in vitro analyses. Intern Med. 2009;48:1799-875 pubmed
    ..We also examined the effect of some antiretroviral drugs (ARVs) on adipocytes in vitro...
  74. Takahashi M, Ishida Y, Iwaki D, Kanno K, Suzuki T, Endo Y, et al. Essential role of mannose-binding lectin-associated serine protease-1 in activation of the complement factor D. J Exp Med. 2010;207:29-37 pubmed publisher
    ..Thus, it is clear that MASP-1 is an essential protease of both the lectin and alternative complement pathways...
  75. Haberland M, Carrer M, Mokalled M, Montgomery R, Olson E. Redundant control of adipogenesis by histone deacetylases 1 and 2. J Biol Chem. 2010;285:14663-70 pubmed publisher
    ..These findings unveil a previously unrecognized role for HDACs in the control of adipogenesis...
  76. Xiao J, Wang N, Sun B, Cai G. Estrogen receptor mediates the effects of pseudoprotodiocsin on adipogenesis in 3T3-L1 cells. Am J Physiol Cell Physiol. 2010;299:C128-38 pubmed publisher
    ..Our results suggest that PPD inhibits adipogenesis in an ER-dependent manner and induces the expression of ERalpha. These findings may provide a lead toward a novel agent that can be used to treat obesity...
  77. Imhoff B, Hansen J. Extracellular redox environments regulate adipocyte differentiation. Differentiation. 2010;80:31-9 pubmed publisher
    ..Data presented here show that adipocytes/excessive adipose preferentially alter extracellular E(h) to a more oxidized state in vivo and in vitro and may promote further adipogenesis...
  78. Chen C, Zhang Y, Huang C. Berberine inhibits PTP1B activity and mimics insulin action. Biochem Biophys Res Commun. 2010;397:543-7 pubmed publisher
    ..In diabetic mice, BBR lowers hyperglycemia and improves impaired glucose tolerance, but does not increase insulin release and synthesis. The results suggest that BBR represents a different class of anti-hyperglycemic agents...
  79. Gallant M, Odei Addo F, Frost C, Levendal R. Biological effects of THC and a lipophilic cannabis extract on normal and insulin resistant 3T3-L1 adipocytes. Phytomedicine. 2009;16:942-9 pubmed publisher
  80. Yin J, Zuberi A, Gao Z, Liu D, Liu Z, Ye J. Shilianhua extract inhibits GSK-3beta and promotes glucose metabolism. Am J Physiol Endocrinol Metab. 2009;296:E1275-80 pubmed publisher
    ..We conclude that the F100 may be a bioactive component in the SLH plant. It promotes glucose metabolism in vitro and in vivo. Inhibition of GSK-3beta and NF-kappaB may be the potential mechanism...
  81. Murase T, Misawa K, Haramizu S, Hase T. Catechin-induced activation of the LKB1/AMP-activated protein kinase pathway. Biochem Pharmacol. 2009;78:78-84 pubmed publisher
  82. Amini Z, Boyd B, Doucet J, Ribnicky D, Stephens J. St. John's Wort inhibits adipocyte differentiation and induces insulin resistance in adipocytes. Biochem Biophys Res Commun. 2009;388:146-9 pubmed publisher
    ..However, our studies have revealed that SJW may have a negative impact on adipocyte related diseases by limiting differentiation of preadipocytes and significantly inducing insulin resistance in mature fat cells...
  83. D Apolito M, Du X, Zong H, Catucci A, Maiuri L, Trivisano T, et al. Urea-induced ROS generation causes insulin resistance in mice with chronic renal failure. J Clin Invest. 2010;120:203-13 pubmed publisher
    ..These data suggest that therapeutic targeting of urea-induced ROS may help reduce the high morbidity and mortality caused by end-stage renal disease...
  84. Kamiya T, Hara H, Inagaki N, Adachi T. The effect of hypoxia mimetic cobalt chloride on the expression of EC-SOD in 3T3-L1 adipocytes. Redox Rep. 2010;15:131-7 pubmed publisher
  85. Zhou Q, Peng X, Hu L, Xie D, Zhou M, Hou F. Advanced oxidation protein products inhibit differentiation and activate inflammation in 3T3-L1 preadipocytes. J Cell Physiol. 2010;225:42-51 pubmed publisher
    ..This information might have implication for understanding the impairment of preadipocytes differentiation and fat inflammation seen in metabolic syndrome...
  86. Chung H, Park T. Injectable cellular aggregates prepared from biodegradable porous microspheres for adipose tissue engineering. Tissue Eng Part A. 2009;15:1391-400 pubmed publisher
    ..Immunohistochemical studies showed that the aggregate-derived tissues with porous microspheres displayed histological characteristics similar to adipose tissues 4 weeks after injection...
  87. Choi S, Cha B, Lee Y, Yonezawa T, Teruya T, Nagai K, et al. Magnolol enhances adipocyte differentiation and glucose uptake in 3T3-L1 cells. Life Sci. 2009;84:908-14 pubmed publisher
    ..Its ligands, including thiazolidinediones, improve insulin sensitivity in type 2 diabetes. We investigate the effect of magnolol, an ingredient of Magnolia officinalis on adipogenesis and glucose uptake using 3T3-L1 cells...
  88. Konstantopoulos N, Molero Navajas J. The measurement of GLUT4 translocation in 3T3-L1 adipocytes. Methods Mol Biol. 2009;560:111-35 pubmed publisher
    ..This chapter describes the measurement of GLUT4 translocation in cultured cells and its potential application for both mechanistic and therapeutic studies...
  89. Zhang Y, McGillicuddy F, Hinkle C, O Neill S, Glick J, Rothblat G, et al. Adipocyte modulation of high-density lipoprotein cholesterol. Circulation. 2010;121:1347-55 pubmed publisher
  90. Ishihara Y, Ohmori K, Mizukawa M, Hasan A, Noma T, Kohno M. Beneficial direct adipotropic actions of pitavastatin in vitro and their manifestations in obese mice. Atherosclerosis. 2010;212:131-8 pubmed publisher
    ..Herein, we investigated the impacts of pitavastatin at clinically relevant doses on adipose dysfunction and insulin resistance...
  91. Kalupahana N, Claycombe K, Newman S, Stewart T, Siriwardhana N, Matthan N, et al. Eicosapentaenoic acid prevents and reverses insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation. J Nutr. 2010;140:1915-22 pubmed publisher
    ..In C57BL/6J mice, EPA minimized saturated fat-induced insulin resistance and this is in part mediated by its effects on fatty acid oxidation and inflammation...
  92. Karschner V, Pekala P. HuR involvement in mitotic clonal expansion during acquisition of the adipocyte phenotype. Biochem Biophys Res Commun. 2009;383:203-5 pubmed publisher
    ..In the present study, we begin to identify mRNA ligands of HuR whose regulated expression is necessary for adipogenesis...