swiss 3t3 cells


Summary: A cell line established in 1962 from disaggregated Swiss albino mouse embryos. This fibroblast cell line is extremely popular in research.

Top Publications

  1. Kole T, Tseng Y, Huang L, Katz J, Wirtz D. Rho kinase regulates the intracellular micromechanical response of adherent cells to rho activation. Mol Biol Cell. 2004;15:3475-84 pubmed
    ..We observe that the cytoplasmic stiffness and viscosity of serum-starved Swiss 3T3 cells transiently and locally enhances upon treatment with lysophosphatidic acid, and this mechanical behavior ..
  2. See V, Rajala N, Spiller D, White M. Calcium-dependent regulation of the cell cycle via a novel MAPK--NF-kappaB pathway in Swiss 3T3 cells. J Cell Biol. 2004;166:661-72 pubmed
    ..was used to specifically disrupt the transient rise in calcium induced by serum stimulation of starved Swiss 3T3 cells. The serum-induced intracellular calcium peak was essential for subsequent NF-kappaB activation (measured by ..
  3. Rosso F, Marino G, Muscariello L, Cafiero G, Favia P, D Aloia E, et al. Adhesion and proliferation of fibroblasts on RF plasma-deposited nanostructured fluorocarbon coatings: evidence of FAK activation. J Cell Physiol. 2006;207:636-43 pubmed
    ..Also the morphology of the adhering fibroblasts (60 min) adhering on ROUGH surfaces was found quite different compared to cells adhering on smooth ones...
  4. Stewart W, Pearcy L, Floyd Z, Stephens J. STAT5A expression in Swiss 3T3 cells promotes adipogenesis in vivo in an athymic mice model system. Obesity (Silver Spring). 2011;19:1731-4 pubmed publisher
    ..To determine whether STAT5A also plays a role in adipogenesis in vivo, we injected athymic mice with Swiss 3T3 cells expressing an empty pBABE retrovirus, Swiss cells expressing a pBABE retrovirus-containing STAT5A, or 3T3-..
  5. Villena J, Berndt C, Granes F, Reina M, Vilaró S. Syndecan-2 expression enhances adhesion and proliferation of stably transfected Swiss 3T3 cells. Cell Biol Int. 2003;27:1005-10 pubmed
    ..We examined the influence of stable syndecan-2 expression in Swiss 3T3 cells on cell-adhesion and proliferation...
  6. Sinnett Smith J, Zhukova E, Hsieh N, Jiang X, Rozengurt E. Protein kinase D potentiates DNA synthesis induced by Gq-coupled receptors by increasing the duration of ERK signaling in swiss 3T3 cells. J Biol Chem. 2004;279:16883-93 pubmed
    ..Here, we examined whether PKD overexpression in Swiss 3T3 cells regulates the activation/inactivation kinetics of the extracellular-regulated protein kinase (ERK) in ..
  7. Nichols R, Dzamko N, Morrice N, Campbell D, Deak M, Ordureau A, et al. 14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization. Biochem J. 2010;430:393-404 pubmed publisher
    ..Consistent with this, endogenous LRRK2 isolated from Swiss 3T3 cells or various mouse tissues is associated with endogenous 14-3-3 isoforms...
  8. Babb R, Homer K, Robbins J, Lax A. Modification of heterotrimeric G-proteins in Swiss 3T3 cells stimulated with Pasteurella multocida toxin. PLoS ONE. 2012;7:e47188 pubmed publisher
    ..We have investigated modification of native G? subunits mediated by PMT in Swiss 3T3 cells using 2-D gel electrophoresis and antibody detection...
  9. Oubrahim H, Wong A, Wilson B, Chock P. Pasteurella multocida toxin (PMT) upregulates CTGF which leads to mTORC1 activation in Swiss 3T3 cells. Cell Signal. 2013;25:1136-48 pubmed publisher
    ..Our findings reveal that CTGF plays an important role, but there are additional factors involved in the mitogenic action of PMT. ..

More Information

Publications108 found, 100 shown here

  1. Santiskulvong C, Sinnett Smith J, Rozengurt E. Insulin reduces the requirement for EGFR transactivation in bombesin-induced DNA synthesis. Biochem Biophys Res Commun. 2004;318:826-32 pubmed
    The binding of bombesin to its cognate G-protein coupled receptor stimulates quiescent Swiss 3T3 cells to re-initiate DNA synthesis and cell division...
  2. Voth D, Ballard J. Clostridium difficile toxins: mechanism of action and role in disease. Clin Microbiol Rev. 2005;18:247-63 pubmed
    ..Collectively, the present review provides a comprehensive update on TcdA and TcdB's mechanism of action as well as the role of these toxins in disease. ..
  3. Simboeck E, Sawicka A, Zupkovitz G, Senese S, Winter S, Dequiedt F, et al. A phosphorylation switch regulates the transcriptional activation of cell cycle regulator p21 by histone deacetylase inhibitors. J Biol Chem. 2010;285:41062-73 pubmed publisher
  4. Miyamoto Y, Tanabe M, Date K, Sakuda K, Sano K, Ogawa H. Sialylation of vitronectin regulates stress fiber formation and cell spreading of dermal fibroblasts via a heparin-binding site. Glycoconj J. 2016;33:227-36 pubmed publisher
    ..the properties of mouse primary cultured dermal fibroblasts (MDF) and a dermal fibroblast cell line, Swiss 3T3 cells. At first, we confirmed that VN from PH rats or de-sialylated VN also decreased cell spreading in MDF and ..
  5. Faucherre A, Desbois P, Satre V, Lunardi J, Dorseuil O, Gacon G. Lowe syndrome protein OCRL1 interacts with Rac GTPase in the trans-Golgi network. Hum Mol Genet. 2003;12:2449-56 pubmed
    ..The OCRL1 RhoGAP domain showed low Rac GAP activity in vitro, and when expressed in Swiss 3T3 cells induced specific inhibition of RacGTP dependent ruffles, consistent with OCRL1 being an active RacGAP...
  6. Martinez Quiles N, Ho H, Kirschner M, Ramesh N, Geha R. Erk/Src phosphorylation of cortactin acts as a switch on-switch off mechanism that controls its ability to activate N-WASP. Mol Cell Biol. 2004;24:5269-80 pubmed
  7. Caprio M, Feve B, Claës A, Viengchareun S, Lombes M, Zennaro M. Pivotal role of the mineralocorticoid receptor in corticosteroid-induced adipogenesis. FASEB J. 2007;21:2185-94 pubmed
    ..MR thus may be of pathophysiological relevance to the development of obesity and the metabolic syndrome. ..
  8. Takeuchi T, Wang L, Mori S, Nakagawa K, Yoshikura H, Kanda T. Characterization of mouse 3T3-swiss albino cells available in Japan: necessity of quality control when used as feeders. Jpn J Infect Dis. 2008;61:9-12 pubmed
    ..The data support the guidelines that recommend the quality control of each lot of 3T3-Swiss albino cells if they are to be used clinically. ..
  9. Huang L, Haylor J, Fisher M, Hau Z, El Nahas A, Griffin M, et al. Do changes in transglutaminase activity alter latent transforming growth factor beta activation in experimental diabetic nephropathy?. Nephrol Dial Transplant. 2010;25:3897-910 pubmed publisher
    ..Regulation of TG2 directly influences the level of active TGF-?1, and thus, TG inhibition may exert a renoprotective effect by targeting not only a direct extracellular matrix deposition but also TGF-?1 activation and recruitment. ..
  10. Kutuk O, Poli G, Basaga H. Resveratrol protects against 4-hydroxynonenal-induced apoptosis by blocking JNK and c-JUN/AP-1 signaling. Toxicol Sci. 2006;90:120-32 pubmed
    ..In this respect, resveratrol acting through MAP kinase pathways and specifically on JNK could have a role other than acting as an antioxidant-quenching reactive oxygen intermediate. ..
  11. Lo H, Hsu S, Xia W, Cao X, Shih J, Wei Y, et al. Epidermal growth factor receptor cooperates with signal transducer and activator of transcription 3 to induce epithelial-mesenchymal transition in cancer cells via up-regulation of TWIST gene expression. Cancer Res. 2007;67:9066-76 pubmed
    ..Together, we report here that EGF/EGFR signaling pathways induce cancer cell EMT via STAT3-mediated TWIST gene expression. ..
  12. Hobbs G, Mitchell L, Arrington M, Gunawardena H, Decristo M, Loeser R, et al. Redox regulation of Rac1 by thiol oxidation. Free Radic Biol Med. 2015;79:237-50 pubmed publisher
    ..Expression of Rac1(C18D) in Swiss 3T3 cells showed greatly enhanced GTP-bound Rac1 relative to Rac1(WT) and the redox-insensitive Rac1(C18S) variant...
  13. Waldron R, Rey O, Zhukova E, Rozengurt E. Oxidative stress induces protein kinase C-mediated activation loop phosphorylation and nuclear redistribution of protein kinase D. J Biol Chem. 2004;279:27482-93 pubmed
    ..phosphorylation to mediate dose- and time-dependent activation of PKD, both endogenously expressed in Swiss 3T3 cells and stably overexpressed in Swiss 3T3-GFP.PKD cells...
  14. Rolli Derkinderen M, Sauzeau V, Boyer L, Lemichez E, Baron C, Henrion D, et al. Phosphorylation of serine 188 protects RhoA from ubiquitin/proteasome-mediated degradation in vascular smooth muscle cells. Circ Res. 2005;96:1152-60 pubmed
  15. Harada I, Kim S, Cho C, Kurosawa H, Akaike T. A simple combined floating and anchored collagen gel for enhancing mechanical strength of culture system. J Biomed Mater Res A. 2007;80:123-30 pubmed
    ..This simple method will be applied as a convenient bioreactor to study cellular processes of the fibroblasts in the tissues with highly oriented fibrils such as ligaments or tendons. ..
  16. Marquis B, McFarland A, Braun K, Haynes C. Dynamic measurement of altered chemical messenger secretion after cellular uptake of nanoparticles using carbon-fiber microelectrode amperometry. Anal Chem. 2008;80:3431-7 pubmed publisher
  17. Ding W, Warburton D. Down-regulation of Sprouty2 via p38 MAPK plays a key role in the induction of cellular apoptosis by tumor necrosis factor-alpha. Biochem Biophys Res Commun. 2008;375:460-4 pubmed publisher
    ..down-regulation of Spry2 by gene overexpression led to reverse of the apoptotic effect of TNF-alpha in Swiss 3T3 cells. To our knowledge, this study is the first that reported the association of Spry2 with TNF-alpha signaling ..
  18. Tominaga A, Sugawara H, Futagawa T, Inoue K, Sasaki K, Minamino N, et al. Characterization of the testis-specific promoter region in the human pituitary adenylate cyclase-activating polypeptide (PACAP) gene. Genes Cells. 2010;15:595-606 pubmed publisher
    ..By using their siRNAs, the depletion of their proteins in F9 cells affected the potent promoter activity of the 80-bp fragment, suggesting that they might be involved in the testis-specific gene expression of PACAP. ..
  19. Grantham J, Brackley K, Willison K. Substantial CCT activity is required for cell cycle progression and cytoskeletal organization in mammalian cells. Exp Cell Res. 2006;312:2309-24 pubmed
    ..processing by CCT in vitro, causes a delay in cell cycle progression through G1/S phase in synchronized Swiss 3T3 cells. The degree of growth arrest strongly correlates with the extent of CCT depletion, indicating that full CCT ..
  20. Ito G, Iwatsubo T. Re-examination of the dimerization state of leucine-rich repeat kinase 2: predominance of the monomeric form. Biochem J. 2012;441:987-94 pubmed publisher
    ..Collectively, we conclude that the monomer form of LRRK2 is predominant within cells, and that dimerization is dispensable for its enzymatic activity. ..
  21. Watt S, Kimber W, Fleming I, Leslie N, Downes C, Lucocq J. Detection of novel intracellular agonist responsive pools of phosphatidylinositol 3,4-bisphosphate using the TAPP1 pleckstrin homology domain in immunoelectron microscopy. Biochem J. 2004;377:653-63 pubmed
    ..These data suggest a role for PtdIns(3,4) P (2) in endomembrane function. ..
  22. Rosso F, Marino G, Grimaldi A, Cafiero G, Chiellini E, Chiellini F, et al. Vitronectin absorbed on nanoparticles mediate cell viability/proliferation and uptake by 3T3 Swiss albino mouse fibroblasts: in vitro study. Biomed Res Int. 2013;2013:539348 pubmed publisher
    ..e., Vitronectin) that represent an external stimuli, switched to the nucleus by FAK enzyme, which in turn modulate fibroblasts viability/proliferation. ..
  23. Tirado O, Mateo Lozano S, Sanders S, Dettin L, Notario V. The PCPH oncoprotein antagonizes the proapoptotic role of the mammalian target of rapamycin in the response of normal fibroblasts to ionizing radiation. Cancer Res. 2003;63:6290-8 pubmed
    ..These results indicate that alterations in PCPH expression may render tumor cells resistant to IR, and perhaps other DNA-damaging agents, by preventing mTOR activation and signaling. ..
  24. Lebrasseur N, Kelly M, Tsao T, Farmer S, Saha A, Ruderman N, et al. Thiazolidinediones can rapidly activate AMP-activated protein kinase in mammalian tissues. Am J Physiol Endocrinol Metab. 2006;291:E175-81 pubmed
    ..They suggest that this effect is associated with a change in cellular energy state and that it is not dependent on PPARgamma-mediated gene transcription. ..
  25. Mackley J, Ando J, Herzyk P, Winder S. Phenotypic responses to mechanical stress in fibroblasts from tendon, cornea and skin. Biochem J. 2006;396:307-16 pubmed
    ..These findings provide the first in-depth analysis of phenotypic differences between fibroblast cells from different tissue sources and reveal the responses of these cells to mechanical stress. ..
  26. Kairisalo M, Korhonen L, Blomgren K, Lindholm D. X-linked inhibitor of apoptosis protein increases mitochondrial antioxidants through NF-kappaB activation. Biochem Biophys Res Commun. 2007;364:138-44 pubmed
    ..These findings disclose a novel role for XIAP in control of oxidative stress and mitochondrial antioxidants that may contribute to cell protection after various injuries. ..
  27. Lee E, Chan E, Yousaf M. Spatio-temporal control of cell coculture interactions on surfaces. Chembiochem. 2009;10:1648-53 pubmed publisher
    ..These dynamic substrates can present a variety of ligands on the surface for biospecific interactions between the ligands and cell surface receptors. Photopatterning enables the ligands to be immobilized in patterns and even gradients. ..
  28. Murphy L, Mackeigan J, Blenis J. A network of immediate early gene products propagates subtle differences in mitogen-activated protein kinase signal amplitude and duration. Mol Cell Biol. 2004;24:144-53 pubmed
    ..As a result, downstream target gene expression and cell cycle progression are significantly changed. ..
  29. Sundstøl Eriksen G, Pettersson H, Lundh T. Comparative cytotoxicity of deoxynivalenol, nivalenol, their acetylated derivatives and de-epoxy metabolites. Food Chem Toxicol. 2004;42:619-24 pubmed
    ..The results verify previous findings that the de-epoxidation is a detoxification reaction. ..
  30. Peterson L, Rajfur Z, Maddox A, Freel C, Chen Y, Edlund M, et al. Simultaneous stretching and contraction of stress fibers in vivo. Mol Biol Cell. 2004;15:3497-508 pubmed
    ..In the periphery, the banding patterns for both proteins were shorter, whereas in central regions, where stretching occurred, the bands were wider. ..
  31. Harris E, Gauvin T, Heimsath E, Higgs H. Assembly of filopodia by the formin FRL2 (FMNL3). Cytoskeleton (Hoboken). 2010;67:755-72 pubmed publisher
    ..The data suggest that the FH2 domain of FRL2 possesses properties not shared by FRL1 that allow it to generate filopodia...
  32. Schmidt C, Murillo R, Bruhn T, Bringmann G, Goettert M, Heinzmann B, et al. Catechin derivatives from Parapiptadenia rigida with in vitro wound-healing properties. J Nat Prod. 2010;73:2035-41 pubmed publisher
    ..Compounds 5 and 7 displayed promising wound-healing effects in a scratch assay. Some of the catechin derivatives showed inhibitory effects on NF-?B DNA binding and p38? MAPK activity...
  33. Hong M, Wilkes M, Penheiter S, Gupta S, Edens M, Leof E. Non-Smad transforming growth factor-? signaling regulated by focal adhesion kinase binding the p85 subunit of phosphatidylinositol 3-kinase. J Biol Chem. 2011;286:17841-50 pubmed publisher
  34. Gaharwar A, Rivera C, Wu C, Schmidt G. Transparent, elastomeric and tough hydrogels from poly(ethylene glycol) and silicate nanoparticles. Acta Biomater. 2011;7:4139-48 pubmed publisher
  35. Coppens I, Joiner K. Host but not parasite cholesterol controls Toxoplasma cell entry by modulating organelle discharge. Mol Biol Cell. 2003;14:3804-20 pubmed
    ..These data define a new mechanism by which host cholesterol specifically controls entry of an intracellular pathogen. ..
  36. El Messaoudi S, Fabbrizio E, Rodriguez C, Chuchana P, Fauquier L, Cheng D, et al. Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the Cyclin E1 gene. Proc Natl Acad Sci U S A. 2006;103:13351-6 pubmed
    ..Finally, we show that grade-3 breast tumors present coelevated mRNA levels of ACTR and CARM1, along with their transcriptional target CCNE1. All together, our results indicate that CARM1 is an important regulator of the CCNE1 gene. ..
  37. Battini L, Fedorova E, Macip S, Li X, Wilson P, Gusella G. Stable knockdown of polycystin-1 confers integrin-alpha2beta1-mediated anoikis resistance. J Am Soc Nephrol. 2006;17:3049-58 pubmed
    ..Furthermore, these findings indicate that reduction of PC1 expression levels, rather than the loss of heterozygosity, may be sufficient to induce cystogenesis. ..
  38. Ding W, Shi W, Bellusci S, Groffen J, Heisterkamp N, Minoo P, et al. Sprouty2 downregulation plays a pivotal role in mediating crosstalk between TGF-beta1 signaling and EGF as well as FGF receptor tyrosine kinase-ERK pathways in mesenchymal cells. J Cell Physiol. 2007;212:796-806 pubmed
    ..we report that transforming growth factor-beta1 (TGF-beta1), which stimulates the growth of quiescent Swiss 3T3 cells, induced a dose dependent decrease of mouse Spry2 protein level within 24-h of treatment, and this effect was ..
  39. Ndika J, Lusink V, Beaubrun C, Kanhai W, Martínez Muñoz C, Jakobs C, et al. Cloning and characterization of the promoter regions from the parent and paralogous creatine transporter genes. Gene. 2014;533:488-93 pubmed publisher
    ..To the best of our knowledge, this is the first experimental evidence of a pseudogene with stronger promoter activity than its parental gene. ..
  40. Schumann M, Nakagawa T, Mantey S, Howell B, Jensen R. Function of non-visual arrestins in signaling and endocytosis of the gastrin-releasing peptide receptor (GRP receptor). Biochem Pharmacol. 2008;75:1170-85 pubmed publisher
    ..Our results show that both non-visual arrestins play an important role in GRP-R internalization and desensitization. ..
  41. Klint P, Hellman U, Wernstedt C, Aman P, Ron D, Claesson Welsh L. Translocated in liposarcoma (TLS) is a substrate for fibroblast growth factor receptor-1. Cell Signal. 2004;16:515-20 pubmed
    ..We show that TLS is tyrosine phosphorylated in intact cells by a number of different growth factors, indicating a role in growth regulation. ..
  42. Song B, Yarar D, Schmid S. An assembly-incompetent mutant establishes a requirement for dynamin self-assembly in clathrin-mediated endocytosis in vivo. Mol Biol Cell. 2004;15:2243-52 pubmed
    ..Together these results provide new structural insight into the role of GED in self-assembly and assembly-stimulated GTPase activity and establish that dynamin self-assembly is essential for clathrin-mediated endocytosis. ..
  43. Dastan T, Turan K. In vitro characterization and delivery of chitosan-DNA microparticles into mammalian cells. J Pharm Pharm Sci. 2004;7:205-14 pubmed
    ..However, transfection efficiency of chitosan-DNA microparticles is low and dependent on the cell type. ..
  44. Andrei G, van den Oord J, Fiten P, Opdenakker G, De Wolf Peeters C, De Clercq E, et al. Organotypic epithelial raft cultures as a model for evaluating compounds against alphaherpesviruses. Antimicrob Agents Chemother. 2005;49:4671-80 pubmed
    ..Since no animal model is available for the evaluation of antiviral agents against VZV, the organotypic cultures may be considered a model to evaluate the efficacy of new anti-VZV antivirals before clinical trials. ..
  45. Shemesh T, Verkhovsky A, Svitkina T, Bershadsky A, Kozlov M. Role of focal adhesions and mechanical stresses in the formation and progression of the lamellipodium-lamellum interface [corrected]. Biophys J. 2009;97:1254-64 pubmed publisher
    ..In addition, the model suggests a mechanism of nucleation of the dorsal and arclike actin bundles found in the lamellum. ..
  46. Gutiérrez Martínez E, Fernández Ulibarri I, Lázaro Diéguez F, Johannes L, Pyne S, Sarri E, et al. Lipid phosphate phosphatase 3 participates in transport carrier formation and protein trafficking in the early secretory pathway. J Cell Sci. 2013;126:2641-55 pubmed publisher
    ..We conclude that LPP3 participates in the formation of retrograde transport carriers at the ER-Golgi interface, where it transitorily cycles, and during its route to the plasma membrane. ..
  47. Yeo W, Mrksich M. Electroactive self-assembled monolayers that permit orthogonal control over the adhesion of cells to patterned substrates. Langmuir. 2006;22:10816-20 pubmed
  48. Schapper F, Gonçalves J, Oheim M. Fluorescence imaging with two-photon evanescent wave excitation. Eur Biophys J. 2003;32:635-43 pubmed
  49. Gekas J, Hindié M, Faucheux N, Lanvin O, Mazière C, Fuentes V, et al. The inhibition of cell spreading on a cellulose substrate (cuprophan) induces an apoptotic process via a mitochondria-dependent pathway. FEBS Lett. 2004;563:103-7 pubmed
    ..We compared here the behaviour of rounded Swiss 3T3 cells aggregated on a cellulose cuprophan membrane to those cultured on dish polystyrene...
  50. Westcott N, Yousaf M. Chemoselective ligand patterning of electroactive surfaces using microfluidics. Electrophoresis. 2009;30:3381-5 pubmed publisher
    ..These immobilization strategies were characterized by electrochemistry, fluorescence, and utilizing a cell adhesive peptide, cell patterns were generated...
  51. Jan H, Wei M, Peng C, Lin S, Lai P, Shieh M. The use of polyethylenimine-DNA to topically deliver hTERT to promote hair growth. Gene Ther. 2012;19:86-93 pubmed publisher
    ..These studies provide a potential approach for gene therapy of skin disease...
  52. Polson A, Durrett E, Reisman D. A bidirectional promoter reporter vector for the analysis of the p53/WDR79 dual regulatory element. Plasmid. 2011;66:169-79 pubmed publisher
  53. Caesar M, Zach S, Carlson C, Brockmann K, Gasser T, Gillardon F. Leucine-rich repeat kinase 2 functionally interacts with microtubules and kinase-dependently modulates cell migration. Neurobiol Dis. 2013;54:280-8 pubmed publisher
    ..These findings suggest that LRRK2 and microtubules mutually interact both in non-neuronal cells and in neurons, which might contribute to our understanding of its pathogenic effects in Parkinson's disease...
  54. Jia Y, Turek J. Altered NF-kappaB gene expression and collagen formation induced by polyunsaturated fatty acids. J Nutr Biochem. 2005;16:500-6 pubmed
    ..This study raises the possibility that fatty acids may be used as adjuvants in combination with other therapies (e.g., selective targeting of the NF-kappaB pathway) to control collagen formation...
  55. Dekanty A, Sauane M, Cadenas B, Coluccio F, Barrio M, Casala J, et al. Leukemia inhibitory factor induces DNA synthesis in Swiss mouse 3T3 cells independently of cyclin D1 expression through a mechanism involving MEK/ERK1/2 activation. J Biol Chem. 2006;281:6136-43 pubmed
    Leukemia inhibitory factor (LIF) and oncostatin M (OSM) induce DNA synthesis in Swiss 3T3 cells through common signaling mechanism(s), whereas other related cytokines such as interleukin-6 and ciliary neurotrophic factor do not cause ..
  56. Duraffour S, Snoeck R, Krečmerová M, van den Oord J, De Vos R, Holy A, et al. Activities of several classes of acyclic nucleoside phosphonates against camelpox virus replication in different cell culture models. Antimicrob Agents Chemother. 2007;51:4410-9 pubmed
    ..Our results demonstrate that compounds belonging to the newly synthesized ANPs, in addition to cidofovir, represent promising candidates for the treatment of poxvirus infections...
  57. Lopes L, Murphy N, Nornoo A. Enhancement of transdermal delivery of progesterone using medium-chain mono and diglycerides as skin penetration enhancers. Pharm Dev Technol. 2009;14:524-9 pubmed publisher
    ..We conclude that formulations containing 10% MCG in propylene glycol may be a simple and safe method to improve the transdermal delivery of progesterone and promote its use in hormone replacement therapy...
  58. Di Iorio E, Barbaro V, Volpi N, Bertolin M, Ferrari B, Fasolo A, et al. Localization and expression of CHST6 and keratan sulfate proteoglycans in the human cornea. Exp Eye Res. 2010;91:293-9 pubmed publisher
    ..If coupled to the accuracy of HPLC-based assay that we developed to determine the amount of KS in serum, our findings could lead to more targeted therapeutic treatments of the ocular features in MCD patients...
  59. Numajiri Haruki A, Naito T, Nishie T, Saito S, Nagata K. Interferon-inducible antiviral protein MxA enhances cell death triggered by endoplasmic reticulum stress. J Interferon Cytokine Res. 2011;31:847-56 pubmed publisher
    ..Because cell death in virus-infected cells is one of ultimate anti-virus mechanisms, we propose that MxA-enhanced ER stress signaling is a part of the antiviral activity of MxA by accelerating cell death...
  60. Pocsfalvi G, Votta G, De Vincenzo A, Fiume I, Raj D, Marra G, et al. Analysis of secretome changes uncovers an autocrine/paracrine component in the modulation of cell proliferation and motility by c-Myc. J Proteome Res. 2011;10:5326-37 pubmed publisher
  61. Chen H, Su L, González Pagán O, Overton J, Runnels L. A key role for Mg(2+) in TRPM7's control of ROS levels during cell stress. Biochem J. 2012;445:441-8 pubmed publisher
    ..Taken together, these data uncover an essential role for Mg(2+) in TRPM7's control of cell survival and in the regulation of cellular ROS levels. ..
  62. Matsuzawa T, Kuwae A, Yoshida S, Sasakawa C, Abe A. Enteropathogenic Escherichia coli activates the RhoA signaling pathway via the stimulation of GEF-H1. EMBO J. 2004;23:3570-82 pubmed
    ..This report reveals for the first time that a pathogen can exploit the host factor GEF-H1...
  63. Sullivan D, Ferris M, Pociask D, Brody A. Tumor necrosis factor-alpha induces transforming growth factor-beta1 expression in lung fibroblasts through the extracellular signal-regulated kinase pathway. Am J Respir Cell Mol Biol. 2005;32:342-9 pubmed
  64. Riento K, Ridley A. Inhibition of ROCK by RhoE. Methods Enzymol. 2006;406:533-41 pubmed
    ..RhoE itself is phosphorylated by ROCK I, and this may enhance RhoE regulation of ROCK I function. This chapter describes the method used for studying ROCK inhibition by RhoE...
  65. Bartolomé R, Wright N, Molina Ortiz I, Sánchez Luque F, Teixido J. Activated G(alpha)13 impairs cell invasiveness through p190RhoGAP-mediated inhibition of RhoA activity. Cancer Res. 2008;68:8221-30 pubmed publisher
  66. Español A, Goren N, Ribeiro M, Sales M. Nitric oxide synthase 1 and cyclooxygenase-2 enzymes are targets of muscarinic activation in normal and inflamed NIH3T3 cells. Inflamm Res. 2010;59:227-38 pubmed publisher
    ..In this work, we investigate the action of the muscarinic agonist carbachol (CARB) on the expression and function of nitric oxide synthase (NOS) and cyclooxygenase (COX) in fibroblasts under normal or inflammatory conditions...
  67. Cicchetti G, Biernacki M, Farquharson J, Allen P. A ratiometric expressible FRET sensor for phosphoinositides displays a signal change in highly dynamic membrane structures in fibroblasts. Biochemistry. 2004;43:1939-49 pubmed
  68. Benavides T, Mitjans M, Martinez V, Clapés P, Infante M, Clothier R, et al. Assessment of primary eye and skin irritants by in vitro cytotoxicity and phototoxicity models: an in vitro approach of new arginine-based surfactant-induced irritation. Toxicology. 2004;197:229-37 pubmed
    ..Phototoxicity was performed with 12,12-l-acetyl-arginine, the most stable chemical structure. The validated 3T3 NRU photoxicity assay was used and revealed a phototoxic potential...
  69. Shen S, Tucker W, Chapman E, Steinhardt R. Molecular regulation of membrane resealing in 3T3 fibroblasts. J Biol Chem. 2005;280:1652-60 pubmed
    ..The pattern of inhibition by synaptotagmin C2 fragments that we observed cannot be used to specify a vesicle compartment, such as lysosomes, in membrane repair...
  70. Riento K, Totty N, Villalonga P, Garg R, Guasch R, Ridley A. RhoE function is regulated by ROCK I-mediated phosphorylation. EMBO J. 2005;24:1170-80 pubmed
    ..This is the first demonstration of an endogenous Rho family member being phosphorylated in vivo and indicates that phosphorylation is an important mechanism to control the stability and function of this GTPase-deficient Rho protein...
  71. Morishige J, Takai Y, Hirano K, Tanaka T, Satouchi K. Production and protein kinase C activation of diacylglycerols containing polymethylene-interrupted PUFA. Lipids. 2005;40:155-62 pubmed
    ..When Swiss 3T3 cells cultured with sciadonic acid were stimulated with 100 nM of bombesin, 1-stearoyl-2-sciadonoyl-glycerol (G) ..
  72. Yamasaki C, Tateno C, Aratani A, Ohnishi C, Katayama S, Kohashi T, et al. Growth and differentiation of colony-forming human hepatocytes in vitro. J Hepatol. 2006;44:749-57 pubmed
    ..We aimed to demonstrate the presence of CF-PHs in humans and characterize them with respect to growth and differentiation potential...
  73. Wohlmuth H, Deseo M, Brushett D, Thompson D, Macfarlane G, Stevenson L, et al. Diarylheptanoid from Pleuranthodium racemigerum with in vitro prostaglandin E(2) inhibitory and cytotoxic activity. J Nat Prod. 2010;73:743-6 pubmed publisher
    ..6 microM), and MCF7 mammary adenocarcinoma (IC(50) = 56.9 microM). The cytotoxicity of compound 1 closely resembled that of curcumin, in terms of both IC(50) values and dose-response curves...
  74. Dzamko N, Deak M, Hentati F, Reith A, Prescott A, Alessi D, et al. Inhibition of LRRK2 kinase activity leads to dephosphorylation of Ser(910)/Ser(935), disruption of 14-3-3 binding and altered cytoplasmic localization. Biochem J. 2010;430:405-13 pubmed publisher
    ..In the present study we show that treatment of Swiss 3T3 cells or lymphoblastoid cells derived from control or a Parkinson's disease patient harbouring a homozygous LRRK2(..
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    ..Together, these results uncover important aspects of TGF-? signaling that highlight the role of ERBB ligands/receptors as critical mediators in fibroblast responses to this pleiotropic cytokine...
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    ..These results suggest that the enhancing effects of alpha2,8S-T on the proliferation and mobility are differential depending on the cell types, and ganglioside-associating molecules in the individual cell types need to be investigated...
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    ..The cooperative interactions observed among Sprouty isoforms could represent an advanced system that functions to regulate strictly the activation state of the RTK/ERK pathway in mammalian cells...
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    ..The study shows that thrombin potentiates hyposmolarity-induced osmolyte fluxes and RVD by increasing src/EGFR-dependent signaling, in addition to the Ca2+-dependent pathway...
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    ..We also show changes in the Nile Red red/yellow emission ratio of lipid droplets of 3T3 mouse fibroblasts induced by drugs interfering with the cholesterol cycle...
  81. Fish P, Brown A, Evrard E, Roberts L. 7-Sulfonamido-3-benzazepines as potent and selective 5-HT2C receptor agonists: hit-to-lead optimization. Bioorg Med Chem Lett. 2009;19:1871-5 pubmed publisher
    ..Furthermore, representative examples had excellent in vitro ADME properties and good selectivity over ion channel activity...
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    ..These findings are consistent with p53 transcription being induced in S-phase to provide for a rapid DNA-damage response during S-phase of the cell cycle...
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    ..Quantitative real-time PCR indicates these phenomena occur based on differential expression of small GTPases RhoA and Cdc42, but not Rac1. These findings demonstrate a fundamental difference in the recruitment of fibroblasts and MSCs...
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    ..Recent reports show that BDM does not inhibit any non-muscle myosins so far tested, including nm-myosinII, prompting the question, how were these process affected in BDM studies?..
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    ..Zfp-57 mRNA is up-regulated in Schwann cells in response to leukemia inhibitory factor and fibroblast growth factor 2...
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    ..cell cycles, we compared the ability of FGF1, PDGF-AB and serum to induce a second round of DNA synthesis in Swiss 3T3 cells previously exposed to either FGF1, PDGF-AB or serum during the first cell cycle using [14C]- and [3H]..
  87. Linehan S, Rytkonen A, Yu X, Liu M, Holden D. SlyA regulates function of Salmonella pathogenicity island 2 (SPI-2) and expression of SPI-2-associated genes. Infect Immun. 2005;73:4354-62 pubmed
    ..SlyA exhibits partial functional redundancy with OmpR-EnvZ and contributes to the transcriptional response to low osmolarity and the absence of calcium, two environmental stimuli that promote SPI-2 gene expression...
  88. Papazyan R, Rozengurt E, Rey O. The C-terminal tail of protein kinase D2 and protein kinase D3 regulates their intracellular distribution. Biochem Biophys Res Commun. 2006;342:685-9 pubmed
    ..Collectively, these data demonstrate that the short C-terminal tail of these kinases plays a critical role in determining their cytoplasmic/nuclear localization...
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    ..In this study, we have studied in vitro characterization and transfection efficiency of a plasmid encoding hIL-2, pCXWN-hIL-2, complexed to chitosan, polyethylenimine or DOTAP with varying ratios...
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    ..On the contrary, after 1 hour of treatment, a cytotoxic effect is seen only with CSC in DMSO. In cell cultures devoid of CSC, similar results have been obtained after 24 hours with both types of treatment...
  92. Orth J, Lang S, Preuss I, Milligan G, Aktories K. Action of Pasteurella multocida toxin on Galpha(q) is persistent and independent of interaction with G-protein-coupled receptors. Cell Signal. 2007;19:2174-82 pubmed
    ..Pre-treatment of Swiss 3T3 cells with PMT increased bombesin or vasopressin-induced GTPgammaS-binding in cell membranes by about 50 to 150%...