balb 3t3 cells


Summary: Cell lines developed from disaggregated BALB/c mouse embryos. They are extremely sensitive to CONTACT INHIBITION, and highly susceptible to transformation by SV40 VIRUS and murine sarcoma virus (SARCOMA VIRUSES, MURINE).

Top Publications

  1. Smith C, Alexander B. The relative cytotoxicity of personal care preservative systems in Balb/C 3T3 clone A31 embryonic mouse cells and the effect of selected preservative systems upon the toxicity of a standard rinse-off formulation. Toxicol In Vitro. 2005;19:963-9 pubmed
  2. Tsuchiya T, Tanaka Kagawa T, Jinno H, Tokunaga H, Sakimoto K, Ando M, et al. Inorganic arsenic compounds and methylated metabolites induce morphological transformation in two-stage BALB/c 3T3 cell assay and inhibit metabolic cooperation in V79 cell assay. Toxicol Sci. 2005;84:344-51 pubmed
    ..These results suggest that from the viewpoint of human hazard, more attention should be paid to the tumor promoting activity of inorganic arsenic compounds...
  3. Ao L, Liu J, Liu W, Gao L, Hu R, Fang Z, et al. Comparison of gene expression profiles in BALB/c 3T3 transformed foci exposed to tumor promoting agents. Toxicol In Vitro. 2010;24:430-8 pubmed publisher
  4. Ponti J, Munaro B, Fischbach M, Hoffmann S, Sabbioni E. An optimised data analysis for the Balb/c 3T3 cell transformation assay and its application to metal compounds. Int J Immunopathol Pharmacol. 2007;20:673-84 pubmed
    ..The results demonstrated that the statistical analysis by Fisher's exact test, better reflecting the data properties, greatly facilitates the interpretation of Balb/c3T3 CTA data regarding carcinogenic potential...
  5. Mascolo M, Perdichizzi S, Rotondo F, Morandi E, Guerrini A, Silingardi P, et al. BALB/c 3T3 cell transformation assay for the prediction of carcinogenic potential of chemicals and environmental mixtures. Toxicol In Vitro. 2010;24:1292-300 pubmed publisher
    ..Our results confirm the suitability of the improved protocol to discriminate carcinogenic compounds and support the use of BALB/c 3T3 cell transformation assay as a possible alternative to predict carcinogenic risk to humans...
  6. Miolo G, Levorato L, Gallocchio F, Caffieri S, Bastianon C, Zanoni R, et al. In vitro phototoxicity of phenothiazines: involvement of stable UVA photolysis products formed in aqueous medium. Chem Res Toxicol. 2006;19:156-63 pubmed
    ..Because some of these photoproducts correspond to metabolic products of phenothiazines found in humans, it cannot be ruled out that metabolites of phenothiazines can be phototoxic in vivo. ..
  7. Gülden M, Seibert H. The improvement of in vitro cytotoxicity testing for the assessment of acute toxicity in fish. Altern Lab Anim. 2007;35:39-46 pubmed
    ..Based on these results, a pragmatic in vitro testing strategy for estimating the minimal aquatic toxic potency of chemicals is proposed. ..
  8. Honma M, Takahashi T, Asada S, Nakagawa Y, Ikeda A, Yamakage K. In vitro clastogenicity and phototoxicity of fullerene (C(60)) nanomaterials in mammalian cells. Mutat Res. 2012;749:97-100 pubmed publisher
    ..5 and 5mg/ml with and without metabolic activation. In BALB 3T3 cells, C(60) showed no phototoxic potential but the anatase form of titanium oxide did...
  9. Al Hiyasat A, Darmani H, Bashabsheh O. Cytotoxicity of dental casting alloys after conditioning in distilled water. Int J Prosthodont. 2003;16:597-601 pubmed
    ..Conditioning of base-metal alloys, other than those containing Cu, for 168 hours in distilled water makes their cytotoxicity levels comparable to that of the high-noble alloy. ..

More Information

Publications114 found, 100 shown here

  1. Ohmori K, Sasaki K, Asada S, Tanaka N, Umeda M. An assay method for the prediction of tumor promoting potential of chemicals by the use of Bhas 42 cells. Mutat Res. 2004;557:191-202 pubmed
    ..We propose that the Bhas 42 cell transformation method is practical and useful for the detection of tumor promoters. ..
  2. Welling T, Lu G, Csencsits K, Wood S, Jarvinen L, Bishop D. Regulation of alloimmune Th1 responses by the cyclin-dependent kinase inhibitor p21 following transplantation. Surgery. 2008;143:394-403 pubmed publisher
    ..Our data demonstrate that p21 controls T-cell priming and suggest that p21 and other cdk inhibitors may serve as potential targets for therapeutic manipulation of alloimmune responses. ..
  3. Zhao B, Guo Y, Fu A. Genetically engineered epidermal growth factor conjugate crosses cell membrane. Appl Biochem Biotechnol. 2012;166:1463-71 pubmed publisher
    ..The results also implied that two different mechanisms (EGF receptor-mediated endocytosis and direct penetration) might be involved in GST-TAT-EGF transmembrane delivery. ..
  4. Bozkurt T, Larsson O, Adner M. Stimulation of cannabinoid CB1 receptors prevents nerve-mediated airway hyperreactivity in NGF-induced inflammation in mouse airways. Eur J Pharmacol. 2016;776:132-8 pubmed publisher
    ..These findings indicate a protective role of CB1 receptors in airway inflammation. ..
  5. Stasevich T, Mueller F, Brown D, McNally J. Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis. EMBO J. 2010;29:1225-34 pubmed publisher
    ..We anticipate the methods we have developed here will be broadly applicable, particularly for deciphering the binding kinetics of other nuclear proteins that, similar to H1, interact with and modify chromatin. ..
  6. Kashyap V, Gudas L. Epigenetic regulatory mechanisms distinguish retinoic acid-mediated transcriptional responses in stem cells and fibroblasts. J Biol Chem. 2010;285:14534-48 pubmed publisher
    ..We have delineated the complex mechanisms that control RA-mediated transcription in fibroblasts versus stem cells. ..
  7. Giancola C, Ercole C, Fotticchia I, Spadaccini R, Pizzo E, D Alessio G, et al. Structure-cytotoxicity relationships in bovine seminal ribonuclease: new insights from heat and chemical denaturation studies on variants. FEBS J. 2011;278:111-22 pubmed publisher
    ..The data show the importance of Arg80 for the cytotoxicity of BS-RNase, and also support the hypothesis that the reduced derivative of BS-RNase is responsible for its cytotoxic activity. ..
  8. Tsujino T, Nagata T, Katoh F, Yamasaki H. Inhibition of Balb/c 3T3 cell transformation by synthetic acyclic retinoid NIK-333; possible involvement of enhanced gap junctional intercellular communication. Cancer Detect Prev. 2007;31:332-8 pubmed
    ..These data indicate that NIK-333 suppresses chemical carcinogenesis in vitro and support the hypothesis that enhancement of GJIC is involved in this process. ..
  9. Wang W, Huang H, Hou D, Liu P, Wei H, Fu X, et al. Mechanosensitivity of STREX-lacking BKCa channels in the colonic smooth muscle of the mouse. Am J Physiol Gastrointest Liver Physiol. 2010;299:G1231-40 pubmed publisher
    ..We suggest that in addition to the STREX domain, there are other additional structures in the channel responsible for mechanically coupling with the cell membrane. ..
  10. Baek D, Kim T, Lim H, Kang J, Seong S, Choi S, et al. Embryotoxicity assessment of developmental neurotoxicants using a neuronal endpoint in the embryonic stem cell test. J Appl Toxicol. 2012;32:617-26 pubmed publisher
    ..In conclusion, our results indicate that Tuj-1 ID?? can be used as a surrogate endpoint of the traditional EST to screen developmental neurotoxicants correctly and it can also be applied to other chemicals. ..
  11. Nakajima M, Shimada S, Nagai M, Mizuhashi F, Sugiyama C, Masuda S, et al. 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone [MX] shows initiating and promoting activities in a two-stage BALB/c 3T3 cell transformation assay. Mutagenesis. 2005;20:375-9 pubmed
    ..This in vitro cell transformation study with MX adds supportive information to studies showing MX carcinogenicity and tumour promoter activity, and adds mechanistic understanding of the action of MX. ..
  12. Geletu M, Arulanandam R, Chevalier S, Saez B, Larue L, Feracci H, et al. Classical cadherins control survival through the gp130/Stat3 axis. Biochim Biophys Acta. 2013;1833:1947-59 pubmed publisher
  13. Leznev E, Popova I, Lavrovskaja V, Evtodienko Y. Comparison of oxygen consumption rates in minimally transformed BALB/3T3 and virus-transformed 3T3B-SV40 cells. Biochemistry (Mosc). 2013;78:904-8 pubmed publisher
    ..e. there is no electron transfer to oxygen). The high "reserve" respiration is suggested to play an important role in preventing apoptosis of 3T3B-SV40 cells. ..
  14. Wu X, Su Z, Li X, Zheng Q, Huang Y, Yuan H. High-level expression and purification of a nonmitogenic form of human acidic fibroblast growth factor in Escherichia coli. Protein Expr Purif. 2005;42:7-11 pubmed
    ..The purified recombinant nhaFGF was sufficiently prepared and sufficient for the following pharmacological study. ..
  15. Taniwaki N, da Silva C, da Silva S, Mortara R. Distribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective forms. Acta Trop. 2007;103:14-25 pubmed
    ..Beyond the polymorphic distribution of amastigote surface epitopes, Calomys, and mice heart sections presented several inflammatory cells around amastigotes and trypomastigotes nests. ..
  16. Joy J, Pereira J, Aid Launais R, Pavon Djavid G, Ray A, Letourneur D, et al. Gelatin - Oxidized carboxymethyl cellulose blend based tubular electrospun scaffold for vascular tissue engineering. Int J Biol Macromol. 2018;107:1922-1935 pubmed publisher
    ..Crosslinking with biocompatible crosslinker OCMC has rendered insolubility to gelatin yet making it spinable for electrospinning to fabricate porous, nanofibrous vascular biomaterial. ..
  17. Huang J, Chen C, Chuang N. P120-GAP associated with syndecan-2 to function as an active switch signal for Src upon transformation with oncogenic ras. Biochem Biophys Res Commun. 2005;329:855-62 pubmed
    ..japonicus. ..
  18. Ning B, Zhu H, Zhou X, Cao Y, Zhou A. [Prokaryotic expression, purification of prM of JEV and preparation of monoclonal antibody]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008;22:65-7 pubmed
    ..mAb against prM epitope of JEV was prepared successfully. The obtained prM specific mAb was valuable for the prevention and dignosis of Japanese encephalitis. ..
  19. Phan C, Lee G, Macreadie I, Malek S, Pamela D, Sabaratnam V. Lipid constituents of the edible mushroom, Pleurotus giganteus demonstrate anti-Candida activity. Nat Prod Commun. 2013;8:1763-5 pubmed
    ..To our knowledge, this is the first report of antifungal activity of the lipid components of Pleurotus giganteus against Candida species...
  20. Lai C, Mas R, Nair N, Majid M, Mansor S, Navaratnam V. Typhonium flagelliforme inhibits cancer cell growth in vitro and induces apoptosis: an evaluation by the bioactivity guided approach. J Ethnopharmacol. 2008;118:14-20 pubmed publisher
    ..D/F21 was found to be the active and cancer cell line specific fraction of Typhonium flagelliforme. Its major chemical constituents had been determined spectroscopically. ..
  21. Hettwer M, Reis Fernandes M, Iken M, Ott M, Steinberg P, Nau H. Metabolic activation capacity by primary hepatocytes expands the applicability of the embryonic stem cell test as alternative to experimental animal testing. Reprod Toxicol. 2010;30:113-20 pubmed publisher
  22. Mahto S, Park C, Yoon T, Rhee S. Assessment of cytocompatibility of surface-modified CdSe/ZnSe quantum dots for BALB/3T3 fibroblast cells. Toxicol In Vitro. 2010;24:1070-7 pubmed publisher
    ..This study suggests that surface modification by ligands plays a significant role in the prevention of cytotoxicity of QDs. ..
  23. Sung W, Chen P, Liao M, Lee J. Enhanced aerobic glycolysis of nasopharyngeal carcinoma cells by Epstein-Barr virus latent membrane protein 1. Exp Cell Res. 2017;359:94-100 pubmed publisher
    ..In conclusion, our results proved facilitation of the Warburg effect by LMP1 through alteration of mitochondrial function in NPC cells. ..
  24. Kruse M, Kilic B, Flick B, Stahlmann R. Effect of quinupristin/dalfopristin on 3T3 and Eahy926 cells in vitro in comparison to other antimicrobial agents with the potential to induce infusion phlebitis. Arch Toxicol. 2007;81:447-52 pubmed
    ..Thus, these in vitro assays may serve as an estimate for the prediction of local tolerability of antibiotics when administered parenterally. ..
  25. Andoh Y, Suzuki H, Araki M, Mizutani A, Ohashi T, Okumura T, et al. Low- and high-level expressions of heme oxygenase-1 in cultured cells under uninduced conditions. Biochem Biophys Res Commun. 2004;320:722-9 pubmed
    ..Neither prostaglandin H2, E2 nor F2a induced HO-1 expression. These results suggest that some cells respond to the cellular stress and intermediates of prostaglandin biosynthesis may act as endogenous stressors to induce HO-1. ..
  26. Ma Z, Wang Z, Zhang J. [Carcinogenicity of duodenogastric reflux juice in patients undergoing gastrectomy]. Zhonghua Wai Ke Za Zhi. 2001;39:764-6 pubmed
    ..To determine the carcinogenic potential of reflux juice from patients undergoing remote gastrectomy and to clarify the relationship between duodenogastric reflux and gastric stump cancer...
  27. Seidita G, Mirisola M, D Anna R, Gallo A, Jensen R, Mantey S, et al. Analysis of the gastrin-releasing peptide receptor gene in Italian patients with autism spectrum disorders. Am J Med Genet B Neuropsychiatr Genet. 2008;147B:807-13 pubmed publisher
    ..However, there is a potential role of C6S and L181F mutations on GRPR function, and possibly in the pathogenesis of the autistic disorders in the two patients. ..
  28. Maeshima H, Ohno K, Tanaka Azuma Y, Nakano S, Yamada T. Identification of tumor promotion marker genes for predicting tumor promoting potential of chemicals in BALB/c 3T3 cells. Toxicol In Vitro. 2009;23:148-57 pubmed publisher
    ..These results suggest that this tumor promoting activity test system, based on 22 marker genes, can become a valuable tool for screening potential tumor promoters. ..
  29. Kato S, Aoshima H, Saitoh Y, Miwa N. Biological safety of LipoFullerene composed of squalane and fullerene-C60 upon mutagenesis, photocytotoxicity, and permeability into the human skin tissue. Basic Clin Pharmacol Toxicol. 2009;104:483-7 pubmed publisher
    ..Thus, C60 dissolved in squalane may not give any significant biological toxic effects such as photocytotoxicity,bacterial reverse mutagenicity, and permeability into the human skin. ..
  30. Hoffmann S, Hothorn L, Edler L, Kleensang A, Suzuki M, Phrakonkham P, et al. Two new approaches to improve the analysis of BALB/c 3T3 cell transformation assay data. Mutat Res. 2012;744:36-41 pubmed publisher
    ..The scripts of the statistical evaluation programs written in R - a freely available statistical software - are appended including exemplary outputs (Appendix A). ..
  31. Sasaki K, Bohnenberger S, Hayashi K, Kunkelmann T, Muramatsu D, Phrakonkham P, et al. Recommended protocol for the BALB/c 3T3 cell transformation assay. Mutat Res. 2012;744:30-5 pubmed publisher
    ..Use of this protocol and of the associated photo catalogue included in this issue (Sasaki et al. [17]) is recommended for the future conduct of the BALB/c 3T3 CTA. ..
  32. Sabbioni E, Fortaner S, Farina M, Del Torchio R, Olivato I, Petrarca C, et al. Cytotoxicity and morphological transforming potential of cobalt nanoparticles, microparticles and ions in Balb/3T3 mouse fibroblasts: an in vitro model. Nanotoxicology. 2014;8:455-64 pubmed publisher
    ..On the contrary, the role of ions released by particles in culture medium is negligible. Thus, both the chemical and the physical properties of Co particles contribute to cytotoxicity and morphological transformation. ..
  33. Boratynska A, Martyniszyn L, Szulc L, Krzyzowska M, Szczepanowska J, Niemialtowski M. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Acta Virol. 2010;54:41-8 pubmed
    ..i.). These results provided a basis for the further analysis of ECTV motion in the infected cells as well as for impact of ECTV infection on the cytoskeletal proteins...
  34. Tsai Z, Tsai F, Yang W, Wang J, Liu C, Shen C, et al. Preparation and characterization of ferrofluid stabilized with biocompatible chitosan and dextran sulfate hybrid biopolymer as a potential magnetic resonance imaging (MRI) T2 contrast agent. Mar Drugs. 2012;10:2403-14 pubmed publisher
    ..Finally, the cellular uptake and biocompatibility assays indicate that the hybrid polymer encapsulating the SPIONs exhibited great potential as a magnetic resonance imaging T2 contrast agent for cell tracking. ..
  35. Gabrielli F, Salvi R, Garulli C, Kalogris C, Arima S, Tardella L, et al. Identification of relevant conformational epitopes on the HER2 oncoprotein by using Large Fragment Phage Display (LFPD). PLoS ONE. 2013;8:e58358 pubmed publisher
  36. Nelson D, Quispel W, Badalian Very G, van Halteren A, van den Bos C, Bovee J, et al. Somatic activating ARAF mutations in Langerhans cell histiocytosis. Blood. 2014;123:3152-5 pubmed publisher
    ..Mutant ARAF activity was inhibited by vemurafenib, a BRAF inhibitor, indicating the importance of fully evaluating ERK pathway abnormalities in selecting LCH patients for targeted inhibitor therapy. ..
  37. Chiappetta D, Alvarez Lorenzo C, Rey Rico A, Taboada P, Concheiro A, Sosnik A. N-alkylation of poloxamines modulates micellar assembly and encapsulation and release of the antiretroviral efavirenz. Eur J Pharm Biopharm. 2010;76:24-37 pubmed publisher
    ..For all the derivatives evaluated, the in vitro release fitted zero-order kinetics and was sustained for at least 24 h. These findings point out N-alkylated poloxamines as promising nanocarriers for oral or parenteral drug delivery. ..
  38. Zhang Z, Fu J, Yao B, Zhang X, Zhao P, Zhou Z. In vitro genotoxicity of danthron and its potential mechanism. Mutat Res. 2011;722:39-43 pubmed publisher
  39. Lao T, Chen S, Sang N. Two mutations impair the stability and function of ubiquitin-activating enzyme (E1). J Cell Physiol. 2012;227:1561-8 pubmed publisher
    ..Our data establish the molecular basis for unambiguous interpretation of experimental data based on TS20 cells, and provide new insight into the structural determinants of E1 stability. ..
  40. Graepel R, Lamon L, Asturiol D, Berggren E, Joossens E, Paini A, et al. The virtual cell based assay: Current status and future perspectives. Toxicol In Vitro. 2017;45:258-267 pubmed publisher
    ..Further extension of the VCBA is discussed in the second part, exploring potential application to i) manufactured nanomaterials, ii) additional cell lines and endpoints, and considering iii) other opportunities. ..
  41. Lei Y, Joseph P, Wu Z, Ong T. [Effect of aberrant DNA methylation on the expression of cancer-related genes in Cd-transformed cells]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2003;21:114-6 pubmed
    ..DNA hypermethylation is known to result in gene silencing, it appears that hypermethylation of p16 gene may represent a possible epigenetic mechanism for Cd-induced cell transformation and carcinogenesis. ..
  42. Ponti J, Ceriotti L, Munaro B, Farina M, Munari A, Whelan M, et al. Comparison of impedance-based sensors for cell adhesion monitoring and in vitro methods for detecting cytotoxicity induced by chemicals. Altern Lab Anim. 2006;34:515-25 pubmed
    ..The results show that the sensitivity of the developed chip was better than that with the MTT test, with the additional advantages of online monitoring. ..
  43. Zhou W, Zhao M, Zhao Y, Mou Y. A fibrin gel loaded with chitosan nanoparticles for local delivery of rhEGF: preparation and in vitro release studies. J Mater Sci Mater Med. 2011;22:1221-30 pubmed publisher
    ..This novel delivery system may have great potential applications in the local administration of rhEGF. ..
  44. Zagorac G, Mahmutefendić H, Tomas M, Kucić N, Le Bouteiller P, Lucin P. Early endosomal rerouting of major histocompatibility class I conformers. J Cell Physiol. 2012;227:2953-64 pubmed publisher
    ..As a result, the open MHC-I conformers internalized with higher rate than fully conformed counterparts. Thus, our data suggest the existence of conformation-based protein sorting mechanism in the endosomal system. ..
  45. Dijoux N, Guingand Y, Bourgeois C, Durand S, Fromageot C, Combe C, et al. Assessment of the phototoxic hazard of some essential oils using modified 3T3 neutral red uptake assay. Toxicol In Vitro. 2006;20:480-9 pubmed
    ..After comparison of the different systems, five other essential oils were tested with both cell lines. Cellular damage was evaluated by the NRU cytotoxicity test or by MTT conversion test. ..
  46. Mizobuchi N, Hoseki J, Kubota H, Toyokuni S, Nozaki J, Naitoh M, et al. ARMET is a soluble ER protein induced by the unfolded protein response via ERSE-II element. Cell Struct Funct. 2007;32:41-50 pubmed
    ..ARMET is the second fully characterized ERSE-II-dependent gene and likely contributes to quality control of proteins in the ER. ..
  47. Wohlrab S, Muller S, Schmidt A, Neubauer S, Kessler H, Leal Egaña A, et al. Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins. Biomaterials. 2012;33:6650-9 pubmed publisher
    ..Interestingly, the genetically created hybrid protein (with a linear RGD sequence) showed similar or slightly better cell adhesion properties as the silk protein chemically modified with the cyclic RGD peptide. ..
  48. Xia S, Forman L, Faller D. Protein kinase C delta is required for survival of cells expressing activated p21RAS. J Biol Chem. 2007;282:13199-210 pubmed
    ..Selective suppression of PKCdelta activity and consequent induction of apoptosis is a potential strategy for targeting of tumor cells containing an activated p21(RAS). ..
  49. Matsuoka A, Tsuchiya T. Gene expression changes in BALB/3T3 transformants induced by poly(L-lactic acid) or polyurethane films. J Biomed Mater Res A. 2004;68:376-82 pubmed
    ..The number of genes that underwent marked expression change in each transformant was consistent with its malignancy. PLLA induced more malignant transformants than PU, especially in relation to osteosarcoma-like gene expression...
  50. Sirvent A, Boureux A, Simon V, Leroy C, Roche S. The tyrosine kinase Abl is required for Src-transforming activity in mouse fibroblasts and human breast cancer cells. Oncogene. 2007;26:7313-23 pubmed
    ..Targeting these Abl-dependent signalling cascades may be of therapeutic value in breast cancers where Src-like function is important...
  51. Bednarkiewicz A, Rodrigues R, Whelan M. Non-invasive monitoring of cytotoxicity based on kinetic changes of cellular autofluorescence. Toxicol In Vitro. 2011;25:2088-94 pubmed publisher
    ..This work forms the basis for cellular autofluorescence measurements in in vitro toxicity screening assays...
  52. Bohic S, Cotte M, Salome M, Fayard B, Kuehbacher M, Cloetens P, et al. Biomedical applications of the ESRF synchrotron-based microspectroscopy platform. J Struct Biol. 2012;177:248-58 pubmed publisher
    ..Possibilities offered by these techniques in the biomedical field are described through examples of applications performed at the ESRF synchrotron-based microspectroscopy platform (ID21 and ID22 beamlines)...
  53. Chen Y, Shuang B, Tan Y, Meng M, Han P, Mo X, et al. The protein X4 of severe acute respiratory syndrome-associated coronavirus is expressed on both virus-infected cells and lung tissue of severe acute respiratory syndrome patients and inhibits growth of Balb/c 3T3 cell line. Chin Med J (Engl). 2005;118:267-74 pubmed
    ..This study was conducted to demonstrate whether the protein X4 was expressed and its essential function in the process of SARS-CoV infection...
  54. Huang Z, Zheng Q, Wu X, Su Z, Xu H, Tan Y, et al. Enhanced protection of modified human acidic fibroblast growth factor with polyethylene glycol against ischemia/reperfusion-induced retinal damage in rats. Toxicol Lett. 2007;170:146-56 pubmed
    ..The protection is probably mediated by antioxidant protective mechanisms...
  55. Hermida C, Garces C, de Oya M, Cano B, Martínez Costa O, Rivero S, et al. The serum levels of the EGF-like homeotic protein dlk1 correlate with different metabolic parameters in two hormonally different children populations in Spain. Clin Endocrinol (Oxf). 2008;69:216-24 pubmed publisher
    ..These data advocate for a role of dlk1 in the maintenance of lipid homeostasis, and suggest that dlk1 levels may influence the development of cardiovascular disease...
  56. Zhu J, Zhang J, Li Q, Du Y, Qiao B, Hu X. Transplanting of mesenchymal stem cells may affect proliferation and function of CD4(+)T cells in experimental autoimmune encephalomyelitis. Exp Clin Transplant. 2012;10:492-500 pubmed publisher
    ..To research the effects of transplanting mesenchymal stem cells on the development of experimental autoimmune encephalomyelitis and the mechanism behind it...
  57. Obitsu S, Sakata K, Teshima R, Kondo K. Eleostearic acid induces RIP1-mediated atypical apoptosis in a kinase-independent manner via ERK phosphorylation, ROS generation and mitochondrial dysfunction. Cell Death Dis. 2013;4:e674 pubmed publisher
    ..Nec1 blocked the interaction of MEK with ERK upon ESA stimulation. Together, these findings provide a new finding that ERK and kinase-independent RIP1 proteins are implicated in atypical ESA-mediated apoptosis. ..
  58. Vietti G, Ibouraadaten S, Palmai Pallag M, Yakoub Y, Bailly C, Fenoglio I, et al. Towards predicting the lung fibrogenic activity of nanomaterials: experimental validation of an in vitro fibroblast proliferation assay. Part Fibre Toxicol. 2013;10:52 pubmed publisher
    ..We developed and validated experimentally here a simple and rapid in vitro assay to evaluate the capacity of a nanomaterial to exert a direct pro-fibrotic effect on fibroblasts...
  59. Liu Q, Nilsen Hamilton M, Xiong S. Synergistic regulation of the acute phase protein SIP24/24p3 by glucocorticoid and pro-inflammatory cytokines. Sheng Li Xue Bao. 2003;55:525-9 pubmed
    ..Finally, the fact that SIP24/24p3 protein can be induced by both pro-inflammatory as well as anti-inflammatory factors is indicative of the important role of SIP24/24p3 in the entire acute phase response process...
  60. Tsai N, Lin S, Lee C, Chen S, Su H, Chang W, et al. The antitumor effects of Angelica sinensis on malignant brain tumors in vitro and in vivo. Clin Cancer Res. 2005;11:3475-84 pubmed
    ..In this study, we have examined the antitumor effects of chloroform extract of Angelica sinensis (AS-C), a traditional Chinese medicine, on glioblastoma multiforme (GBM) brain tumors in vitro and in vivo...
  61. Goldmann O, von Köckritz Blickwede M, Höltje C, Chhatwal G, Geffers R, Medina E. Transcriptome analysis of murine macrophages in response to infection with Streptococcus pyogenes reveals an unusual activation program. Infect Immun. 2007;75:4148-57 pubmed
    ..An understanding of how macrophages respond to S. pyogenes at the molecular level may facilitate the development of new therapeutic paradigms...
  62. Chow K, Wu C, Chang H, Chang C, Chang Y. A simplified tumour model established via Epstein-Barr virus-encoded, nasopharyngeal carcinoma-derived oncogene latent membrane protein 1 in immunocompetent mice. Lab Anim. 2008;42:193-203 pubmed publisher
    ..This novel model of N-LMP1-directed oncogenesis in an immunocompetent environment may serve as an important platform for the future assessment of N-LMP1-targeted tumour therapies...
  63. Javanmoghadam Kamrani S, Keyomarsi K. Synchronization of the cell cycle using lovastatin. Cell Cycle. 2008;7:2434-40 pubmed
    ..Due to its level of synchrony and high yield of synchronous populations of cells, Lovastatin method of cell synchronization can be used for examining gene expression patterns in a variety of different cell lines...
  64. Brackley K, Grantham J. Interactions between the actin filament capping and severing protein gelsolin and the molecular chaperone CCT: evidence for nonclassical substrate interactions. Cell Stress Chaperones. 2011;16:173-9 pubmed publisher
    ..In cultured cells, the levels of CCT monomers affect levels of gelsolin, suggesting an additional link between CCT and the actin cytoskeleton that is mediated via the actin filament severing and capping protein gelsolin...
  65. Mónaco D, Rodriguez A, Pascutti M, Carobene M, Falivene J, Gomez A, et al. T-cell immune responses against Env from CRF12_BF and subtype B HIV-1 show high clade-specificity that can be overridden by multiclade immunizations. PLoS ONE. 2011;6:e17185 pubmed publisher
  66. Ramos Álvarez I, Nakamura T, Mantey S, Moreno P, Nuche Berenguer B, Jensen R. Novel chiral-diazepines function as specific, selective receptor agonists with variable coupling and species variability in human, mouse and rat BRS-3 receptor cells. Peptides. 2016;75:8-17 pubmed publisher
    ..These results demonstrate that chiral diazepine analogs (9G, 9D, 9F, 3F) have high/affinity/potency for the BRS-3 receptor in human and rodent cells, but different coupling-relationships and species differences from a peptide agonist. ..
  67. Sakai A, Kikuchi Y, Muroi M, Masui T, Furihata C, Uchida E, et al. Overexpression of NP95 mRNA by tumor promoters in the promotion phase of a two-stage BALB/3T3 cell transformation assay. Biol Pharm Bull. 2003;26:347-51 pubmed
    ..NP95 mRNA was also increased in the transformed BALB/3T3 cells. Overexpression of NP95 may also participate in the maintenance of the transformed phenotype...
  68. Luo L, Li M, Xia H, Chen B, Wang P, Hao W. [Immunogenicity of a multiple epitope DNA vaccine against hepatitis B virus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20:517-21 pubmed
    ..To study the immunogenicity of a multiple epitope DNA vaccine against hepatitis B virus(HBV)...
  69. Gülden M, Dierickx P, Seibert H. Validation of a prediction model for estimating serum concentrations of chemicals which are equivalent to toxic concentrations in vitro. Toxicol In Vitro. 2006;20:1114-24 pubmed
    ..Deviations did not exceed the factor 3 and were in most cases lower than 2. It is concluded that the extrapolation model is quite well suited to predict equivalent concentrations in serum from in vitro effective concentrations...
  70. Li T, Fan G, Wang W, Li T, Yuan Y. Resveratrol induces apoptosis, influences IL-6 and exerts immunomodulatory effect on mouse lymphocytic leukemia both in vitro and in vivo. Int Immunopharmacol. 2007;7:1221-31 pubmed
    ..Interleukin-6 cellular content and release are suppressed by resveratrol as well as mRNA expression. In conclusion, the data provide new findings with respect to resveratrol mechanism of action to mouse lymphocytic leukemia...
  71. Fabbri P, Bondioli F, Messori M, Bartoli C, Dinucci D, Chiellini F. Porous scaffolds of polycaprolactone reinforced with in situ generated hydroxyapatite for bone tissue engineering. J Mater Sci Mater Med. 2010;21:343-51 pubmed publisher
    ..The possibility to employ the developed composites as scaffolds for bone tissue regeneration was assessed by cytotoxicity test of the PCL/HA composites extracts and cell adhesion and proliferation in vitro studies...
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    ..To investigate the action mechanism of a novel chemical structural aminotetralin derivate, 2-Amino-Nonyl-6-Methoxyl-Tetralin Muriate (10b), against Candida albicans (C albicans) in the ergosterol biosynthetic pathway...
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    ..Therefore, knowledge on the species-dependent in vivo kinetics is essential when using in vitro toxicity data for the estimation of in vivo developmental toxicity potencies within series of related compounds...
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    ..The reported data support an overall evaluation of reduced exposure to HPHC and biological activity...
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    ..We designed an in vivo electrogene transfer (EGT) model for delivering antisense TS plasmid (ATS) into tumor to increase the subsequent efficacy of (131)I-IdUrd therapy...
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    ..Specifically, ADE did not occur with FcgammaR-negative cells, did not require active complement proteins, and did not occur on FcgammaR-positive murine cell lines when virions were bound by murine IgG-derived F(ab')2 fragments...
  78. Mahalingam M, Arvind R, Ida H, Murugan A, Yamaguchi M, Tsuchida N. ERK2 CD domain mutation from a human cancer cell line enhanced anchorage-independent cell growth and abnormality in Drosophila. Oncol Rep. 2008;20:957-62 pubmed
    ..Taken together, these data are consistent with ERK2E322K loss of contact with downstream effectors and its constitutive activation, presenting an oncogenic potential and weak abnormality in differentiation...
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    ..This suggests that chromatin binding by MeCP2 and H1 in vivo should be viewed in the context of competitive multifactorial regulation...
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    ..The ranking in sensitivity of the three cell types for CPZ was dependent on the dose metric used...
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    ..Notably, the compounds showed no cytotoxic activity, encouraging their further evaluation as potential starting points for antibacterial drug development...
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    ..Electronic cigarettes (ECs) are used as alternatives to smoking; however, data on their cytotoxic potential are scarce...
  84. Baderna D, Colombo A, Amodei G, Cantù S, Teoldi F, Cambria F, et al. Chemical-based risk assessment and in vitro models of human health effects induced by organic pollutants in soils from the Olona Valley. Sci Total Environ. 2013;463-464:790-801 pubmed publisher
    ..Moreover, the cell transformation assay strengthened the combined approach giving useful information on carcinogenic potential of mixtures. ..
  85. Messer R, Lockwood P, Wataha J, Lewis J, Norris S, Bouillaguet S. In vitro cytotoxicity of traditional versus contemporary dental ceramics. J Prosthet Dent. 2003;90:452-8 pubmed
    ..Yet, the biocompatibility of ceramics is critical to the long-term success of dental prostheses because ceramics are in close contact with oral tissues for extended periods...
  86. Murakami S, Takashima H, Sato Watanabe M, Chonan S, Yamamoto K, Saitoh M, et al. Ursolic acid, an antagonist for transforming growth factor (TGF)-beta1. FEBS Lett. 2004;566:55-9 pubmed
    ..All these findings taken together show that ursolic acid functions as an antagonist for TGF-beta1. This is the first report to show that a small molecule can inhibit TGF-beta1 receptor binding and influence functions of TGF-beta1...
  87. Jin G, Ikushima T. Frequent occurrence of UVB-induced sister chromatid exchanges in telomere regions and its implication to telomere maintenance. Cytogenet Genome Res. 2004;104:310-4 pubmed
    ..UVB irradiation shortened telomeres and augmented the telomerase activity. The possible implications of the frequent occurrence of SCEs in telomere regions are discussed in connection with the maintenance of telomere integrity...
  88. Nakamura T, Takasugi H, Aizawa T, Yoshida M, Mizuguchi M, Mori Y, et al. Peptide mimics of epidermal growth factor (EGF) with antagonistic activity. J Biotechnol. 2005;116:211-9 pubmed
    ..The present results suggest that the peptide synthesized in this study may mimic the epidermal growth factor receptor-binding region in epidermal growth factor...
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    ..While the alternate method is particularly efficient for stable transformation of cells with circular plasmid DNA, both protocols yield similar results for transformation with linear plasmid or genomic DNA, or for transient expression...
  90. De Smedt A, Steemans M, De Boeck M, Peters A, van der Leede B, Van Goethem F, et al. Optimisation of the cell cultivation methods in the embryonic stem cell test results in an increased differentiation potential of the cells into strong beating myocard cells. Toxicol In Vitro. 2008;22:1789-96 pubmed publisher
    ..Testing of 6 reference compounds in the optimized EST showed that the cell culture modifications did not changed the in vitro classification...
  91. Terada M, Abe S, Akasaka T, Uo M, Kitagawa Y, Watari F. Multiwalled carbon nanotube coating on titanium. Biomed Mater Eng. 2009;19:45-52 pubmed publisher
    ..MC3T3-E1 cells were cultured on the MWCNT-coated Ti plate, and showed good cell proliferation and strong cell adhesion. Therefore, the MWCNT coating for titanium could be useful for improvement of cell adhesion on titanium implants...
  92. Ogawa M, Kosaka N, Longmire M, Urano Y, Choyke P, Kobayashi H. Fluorophore-quencher based activatable targeted optical probes for detecting in vivo cancer metastases. Mol Pharm. 2009;6:386-95 pubmed publisher
    ..Thus, these activatable probes, based on the fluorophore-quencher system, hold promise clinically for "see and treat" strategies of cancer management...