cellulosomes

Summary

Summary: Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.

Top Publications

  1. Bayer E, Belaich J, Shoham Y, Lamed R. The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides. Annu Rev Microbiol. 2004;58:521-54 pubmed
  2. Jindou S, Borovok I, Rincon M, Flint H, Antonopoulos D, Berg M, et al. Conservation and divergence in cellulosome architecture between two strains of Ruminococcus flavefaciens. J Bacteriol. 2006;188:7971-6 pubmed
    ..Although the general design of the cluster is identical in the two strains, significant differences in the modular architecture of the scaffoldin proteins were discovered, implying strain-specific divergence in cellulosome organization...
  3. Kang S, Barak Y, Lamed R, Bayer E, Morrison M. The functional repertoire of prokaryote cellulosomes includes the serpin superfamily of serine proteinase inhibitors. Mol Microbiol. 2006;60:1344-54 pubmed
    ..These studies are the first to expand the cellulosome paradigm of protein complex assembly beyond glycoside hydrolase and carbohydrate active enzymes, and to include a newly identified functionality in the Firmicutes...
  4. Hammel M, Fierobe H, Czjzek M, Finet S, Receveur Brechot V. Structural insights into the mechanism of formation of cellulosomes probed by small angle X-ray scattering. J Biol Chem. 2004;279:55985-94 pubmed
    Exploring the mechanism by which the multiprotein complexes of cellulolytic organisms, the cellulosomes, attain their exceptional synergy is a challenge for biologists...
  5. Vazana Y, Moraïs S, Barak Y, Lamed R, Bayer E. Designer cellulosomes for enhanced hydrolysis of cellulosic substrates. Methods Enzymol. 2012;510:429-52 pubmed publisher
    During the past several years, major progress has been accomplished in the production of "designer cellulosomes," artificial enzymatic complexes that were demonstrated to efficiently degrade crystalline cellulose...
  6. Bayer E, Lamed R, White B, Flint H. From cellulosomes to cellulosomics. Chem Rec. 2008;8:364-77 pubmed publisher
    b>Cellulosomes are intricate multienzyme systems produced by several cellulolytic bacteria, the first example of which was discovered in the anaerobic thermophilic bacterium, Clostridium thermocellum...
  7. Nagy T, Tunnicliffe R, Higgins L, Walters C, Gilbert H, Williamson M. Characterization of a double dockerin from the cellulosome of the anaerobic fungus Piromyces equi. J Mol Biol. 2007;373:612-22 pubmed
    ..A central feature of bacterial cellulosomes is a large non-catalytic protein, the scaffoldin, which contains multiple cohesin domains...
  8. Hammel M, Fierobe H, Czjzek M, Kurkal V, Smith J, Bayer E, et al. Structural basis of cellulosome efficiency explored by small angle X-ray scattering. J Biol Chem. 2005;280:38562-8 pubmed
  9. Molinier A, Nouailler M, Valette O, Tardif C, Receveur Brechot V, Fierobe H. Synergy, structure and conformational flexibility of hybrid cellulosomes displaying various inter-cohesins linkers. J Mol Biol. 2011;405:143-57 pubmed publisher
    b>Cellulosomes are large extracellular multi-enzyme complexes that exhibit elevated activity on plant cell-wall polysaccharides...

More Information

Publications78

  1. Mingardon F, Chanal A, López Contreras A, Dray C, Bayer E, Fierobe H. Incorporation of fungal cellulases in bacterial minicellulosomes yields viable, synergistically acting cellulolytic complexes. Appl Environ Microbiol. 2007;73:3822-32 pubmed
    ..viable designer minicellulosomes can be produced that include (i) free (noncellulosomal) enzymes, (ii) fungal enzymes combined with bacterial enzymes, and (iii) a type (family 6) of cellulase never known to occur in natural cellulosomes.
  2. Arai T, Matsuoka S, Cho H, Yukawa H, Inui M, Wong S, et al. Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation. Proc Natl Acad Sci U S A. 2007;104:1456-60 pubmed
    ..Both minicellulosomes showed their respective enzymatic activities. We call this phenomenon "intercellular complementation." Interesting implications concerning bacterial cooperation are suggested from these results...
  3. Jindou S, Xu Q, Kenig R, Shulman M, Shoham Y, Bayer E, et al. Novel architecture of family-9 glycoside hydrolases identified in cellulosomal enzymes of Acetivibrio cellulolyticus and Clostridium thermocellum. FEMS Microbiol Lett. 2006;254:308-16 pubmed
    ..Phylogenetic analysis and preliminary site-directed mutagenesis studies suggest that the catalytic module and the CBM3 dyad are distinctive in their sequences and are proposed to constitute a new GH9 architectural theme...
  4. Mingardon F, Chanal A, Tardif C, Bayer E, Fierobe H. Exploration of new geometries in cellulosome-like chimeras. Appl Environ Microbiol. 2007;73:7138-49 pubmed
    ..these engineered enzymes were somewhat less active on crystalline cellulose than the conventional designer cellulosomes containing the parental enzymes...
  5. Fierobe H, Mingardon F, Mechaly A, Belaich A, Rincon M, Pagès S, et al. Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin. J Biol Chem. 2005;280:16325-34 pubmed
    ..In recent work, we reported the self-assembly of a comprehensive set of defined "bifunctional" chimeric cellulosomes. Each complex contained the following: (i) a chimeric scaffoldin possessing a cellulose-binding module and two ..
  6. Perret S, Belaich A, Fierobe H, Belaich J, Tardif C. Towards designer cellulosomes in Clostridia: mannanase enrichment of the cellulosomes produced by Clostridium cellulolyticum. J Bacteriol. 2004;186:6544-52 pubmed
    ..cellulolyticum by using expression vectors. The trans-produced protein was found to be incorporated into the cellulosomes and became one of the major enzymatic components...
  7. Lilly M, Fierobe H, van Zyl W, Volschenk H. Heterologous expression of a Clostridium minicellulosome in Saccharomyces cerevisiae. FEMS Yeast Res. 2009;9:1236-49 pubmed publisher
    ..cerevisiae through emulation of the cellulosome concept. Potentially, Scaf3p-armed yeast could also be developed into an alternative cell surface display strategy with various tailor-made applications...
  8. Haimovitz R, Barak Y, Morag E, Voronov Goldman M, Shoham Y, Lamed R, et al. Cohesin-dockerin microarray: Diverse specificities between two complementary families of interacting protein modules. Proteomics. 2008;8:968-79 pubmed publisher
    ..Using this approach, extensive cross-species interaction among type-II cohesins and dockerins is shown for the first time. Selective intraspecies binding of an archaeal dockerin to two complementary cohesins is also demonstrated...
  9. Pinheiro B, Gilbert H, Sakka K, Sakka K, Fernandes V, Prates J, et al. Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum. Biochem J. 2009;424:375-84 pubmed publisher
    b>Cellulosomes, synthesized by anaerobic microorganisms such as Clostridium thermocellum, are remarkably complex nanomachines that efficiently degrade plant cell wall polysaccharides...
  10. Moraïs S, Barak Y, Caspi J, Hadar Y, Lamed R, Shoham Y, et al. Contribution of a xylan-binding module to the degradation of a complex cellulosic substrate by designer cellulosomes. Appl Environ Microbiol. 2010;76:3787-96 pubmed publisher
    ..Indeed, only designer cellulosomes in which the XBM was maintained as a component of Xyn11A achieved marked enhancement in activity compared to ..
  11. Bayer E, Lamed R, Himmel M. The potential of cellulases and cellulosomes for cellulosic waste management. Curr Opin Biotechnol. 2007;18:237-45 pubmed
    ..Microbes that produce cellulosomes convert lignocelluose to microbial cell mass and products (e.g. ethanol) simultaneously...
  12. Xu Q, Barak Y, Kenig R, Shoham Y, Bayer E, Lamed R. A novel Acetivibrio cellulolyticus anchoring scaffoldin that bears divergent cohesins. J Bacteriol. 2004;186:5782-9 pubmed
  13. Yaniv O, Shimon L, Bayer E, Lamed R, Frolow F. Scaffoldin-borne family 3b carbohydrate-binding module from the cellulosome of Bacteroides cellulosolvens: structural diversity and significance of calcium for carbohydrate binding. Acta Crystallogr D Biol Crystallogr. 2011;67:506-15 pubmed publisher
    ..These observations indicate that despite their conserved function the scaffoldin-borne CBMs are more diverse in their sequences and structures than previously assumed...
  14. Dror T, Rolider A, Bayer E, Lamed R, Shoham Y. Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase. J Bacteriol. 2005;187:2261-6 pubmed
    ..thermocellum endoglucanases is similar to that of the processive endoglucanase celS but differs from that of a major cellulosomal xylanase in that expression of the latter enzyme is independent of the growth rate...
  15. Wen F, Sun J, Zhao H. Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol. Appl Environ Microbiol. 2010;76:1251-60 pubmed publisher
    ..The strain reported here represents a useful engineering platform for developing CBP-enabling microorganisms and elucidating principles of cellulosome construction and mode of action...
  16. Vazana Y, Moraïs S, Barak Y, Lamed R, Bayer E. Interplay between Clostridium thermocellum family 48 and family 9 cellulases in cellulosomal versus noncellulosomal states. Appl Environ Microbiol. 2010;76:3236-43 pubmed publisher
    ..The targeting effect was found to be the major factor responsible for the elevated activity observed for these specific enzyme combinations, whereas the proximity effect appeared to play a negligible role...
  17. Fontes C, Gilbert H. Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. Annu Rev Biochem. 2010;79:655-81 pubmed publisher
    b>Cellulosomes can be described as one of nature's most elaborate and highly efficient nanomachines...
  18. Noach I, Frolow F, Alber O, Lamed R, Shimon L, Bayer E. Intermodular linker flexibility revealed from crystal structures of adjacent cellulosomal cohesins of Acetivibrio cellulolyticus. J Mol Biol. 2009;391:86-97 pubmed publisher
    ..The findings suggest that linkers can play an important role in the assembly, dynamics and function of the cellulosomal components...
  19. Cha J, Matsuoka S, Chan H, Yukawa H, Inui M, Doi R. Effect of multiple copies of cohesins on cellulase and hemicellulase activities of Clostridium cellulovorans mini-cellulosomes. J Microbiol Biotechnol. 2007;17:1782-8 pubmed
    b>Cellulosomes in Clostridium cellulovorans are assembled by the interaction between the repeated cohesin domains of a scaffolding protein (CbpA) and the dockerin domain of enzyme components...
  20. Xu J, Smith J. Probing the mechanism of cellulosome attachment to the Clostridium thermocellum cell surface: computer simulation of the Type II cohesin-dockerin complex and its variants. Protein Eng Des Sel. 2010;23:759-68 pubmed publisher
    ..Moreover, dynamical cross-correlation analysis indicates that the X-module has a dramatic effect on the cohesin-dockerin interaction and is required for the dynamical integrity of the interface...
  21. Rincon M, Cepeljnik T, Martin J, Lamed R, Barak Y, Bayer E, et al. Unconventional mode of attachment of the Ruminococcus flavefaciens cellulosome to the cell surface. J Bacteriol. 2005;187:7569-78 pubmed
    ..This sortase-mediated mechanism for covalent cell-wall anchoring of the cellulosome in R. flavefaciens differs from those reported thus far for any other cellulosome system...
  22. Jindou S, Brulc J, Levy Assaraf M, Rincon M, Flint H, Berg M, et al. Cellulosome gene cluster analysis for gauging the diversity of the ruminal cellulolytic bacterium Ruminococcus flavefaciens. FEMS Microbiol Lett. 2008;285:188-94 pubmed publisher
    ..Moreover, the scaC gene, being short and variable, appears to be a promising functional phylotyping target for metagenomic population studies of R. flavefaciens in the rumen as a function of the individual host animal...
  23. Caspi J, Irwin D, Lamed R, Li Y, Fierobe H, Wilson D, et al. Conversion of Thermobifida fusca free exoglucanases into cellulosomal components: comparative impact on cellulose-degrading activity. J Biotechnol. 2008;135:351-7 pubmed publisher
    b>Cellulosomes are multi-enzyme complexes produced by certain anaerobic bacteria that exhibit efficient degradation of plant cell wall polysaccharides...
  24. Zverlov V, Klupp M, Krauss J, Schwarz W. Mutations in the scaffoldin gene, cipA, of Clostridium thermocellum with impaired cellulosome formation and cellulose hydrolysis: insertions of a new transposable element, IS1447, and implications for cellulase synergism on crystalline cellulose. J Bacteriol. 2008;190:4321-7 pubmed publisher
    ..gel electrophoresis resulted in extracellular protein patterns comparable to those of isolated cellulosomes, except for a missing CipA band...
  25. Bahari L, Gilad Y, Borovok I, Kahel Raifer H, Dassa B, Nataf Y, et al. Glycoside hydrolases as components of putative carbohydrate biosensor proteins in Clostridium thermocellum. J Ind Microbiol Biotechnol. 2011;38:825-32 pubmed publisher
  26. Tsai S, Goyal G, Chen W. Surface display of a functional minicellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and ethanol production. Appl Environ Microbiol. 2010;76:7514-20 pubmed publisher
    ..This result confirms the use of a synthetic biology approach for the synergistic saccharification and fermentation of cellulose to ethanol by using a yeast consortium displaying a functional minicellulosome...
  27. Caspi J, Barak Y, Haimovitz R, Irwin D, Lamed R, Wilson D, et al. Effect of linker length and dockerin position on conversion of a Thermobifida fusca endoglucanase to the cellulosomal mode. Appl Environ Microbiol. 2009;75:7335-42 pubmed publisher
    ..The activities of the resultant designer cellulosomes were compared with an equivalent mixture of wild-type enzymes. In the present work, a fourth T...
  28. Tamaru Y, Miyake H, Kuroda K, Nakanishi A, Kawade Y, Yamamoto K, et al. Genome sequence of the cellulosome-producing mesophilic organism Clostridium cellulovorans 743B. J Bacteriol. 2010;192:901-2 pubmed publisher
    ..This organism degrades native substrates in soft biomass such as corn fiber and rice straw efficiently by producing an extracellular enzyme complex called the cellulosome. Here we report the genome sequence of C. cellulovorans 743B...
  29. Hyeon J, Yu K, Suh D, Suh Y, Lee S, Lee J, et al. Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae. FEMS Microbiol Lett. 2010;310:39-47 pubmed publisher
    ..34 g g(-1) from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes...
  30. Moraïs S, Barak Y, Caspi J, Hadar Y, Lamed R, Shoham Y, et al. Cellulase-xylanase synergy in designer cellulosomes for enhanced degradation of a complex cellulosic substrate. MBio. 2010;1: pubmed publisher
    Designer cellulosomes are precision-engineered multienzyme complexes in which the molecular architecture and enzyme content are exquisitely controlled...
  31. Blouzard J, Coutinho P, Fierobe H, Henrissat B, Lignon S, Tardif C, et al. Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010;10:541-54 pubmed publisher
    ..The composition of the cellulosomes produced by C...
  32. Kosugi A, Amano Y, Murashima K, Doi R. Hydrophilic domains of scaffolding protein CbpA promote glycosyl hydrolase activity and localization of cellulosomes to the cell surface of Clostridium cellulovorans. J Bacteriol. 2004;186:6351-9 pubmed
    CbpA, the scaffolding protein of Clostridium cellulovorans cellulosomes, possesses one family 3 cellulose binding domain, nine cohesin domains, and four hydrophilic domains (HLDs)...
  33. Tsai S, DaSilva N, Chen W. Functional display of complex cellulosomes on the yeast surface via adaptive assembly. ACS Synth Biol. 2013;2:14-21 pubmed publisher
    ..The unique feature of the anchoring and the adaptor scaffoldin strategy to amplify the number of enzymatic subunits can be easily extended to more complex cellulosomal structures to achieve an even higher level of enzyme synergy...
  34. Fan L, Zhang Z, Yu X, Xue Y, Tan T. Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production. Proc Natl Acad Sci U S A. 2012;109:13260-5 pubmed publisher
    ..The construction and cell surface attachment of cellulosomes were accomplished with two individual miniscaffoldins to increase the display level...
  35. Wieczorek A, Martin V. Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis. Microb Cell Fact. 2010;9:69 pubmed publisher
  36. Sun J, Wen F, Si T, Xu J, Zhao H. Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome. Appl Environ Microbiol. 2012;78:3837-45 pubmed publisher
    ..31 g per g of sugar consumed. ..
  37. Adams J, Pal G, Jia Z, Smith S. Mechanism of bacterial cell-surface attachment revealed by the structure of cellulosomal type II cohesin-dockerin complex. Proc Natl Acad Sci U S A. 2006;103:305-10 pubmed
    ..This structure represents a unique mechanism for cell-surface attachment in anaerobic bacteria and provides a rationale for discriminating between type I and type II Coh modules. ..
  38. Noach I, Frolow F, Jakoby H, Rosenheck S, Shimon L, Lamed R, et al. Crystal structure of a type-II cohesin module from the Bacteroides cellulosolvens cellulosome reveals novel and distinctive secondary structural elements. J Mol Biol. 2005;348:1-12 pubmed
    ..The raised ridge formed by beta-strand 5 confers additional distinctive topographic features to the proposed binding interface that collectively distinguish between the type-II and type-I cohesins. ..
  39. Raman B, McKeown C, Rodriguez M, Brown S, Mielenz J. Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation. BMC Microbiol. 2011;11:134 pubmed publisher
    ..thermocellum alters its gene expression to (i) modulate the composition of cellulosomes that are released into the environment with an increased proportion of enzymes than can efficiently degrade ..
  40. Smith S, Bayer E, Czjzek M. Continually emerging mechanistic complexity of the multi-enzyme cellulosome complex. Curr Opin Struct Biol. 2017;44:151-160 pubmed publisher
    ..properties of anaerobic bacteria are harnessed within elegant, marcomolecular assemblages called cellulosomes, in which proteins of complementary activities amass on scaffold protein networks...
  41. Badruna L, Burlat V, Montanier C. CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry. Methods Mol Biol. 2017;1588:181-197 pubmed publisher
    ..Due to their smaller size, CBMs are interesting alternative probes. The aim of this chapter is to describe the use of CBM as probes to explore complex polysaccharide topochemistry in muro and to quantify enzymatic deconstruction. ..
  42. Fierobe H, Mingardon F, Chanal A. Engineering cellulase activity into Clostridium acetobutylicum. Methods Enzymol. 2012;510:301-16 pubmed publisher
    ..Heterologous secretion of the latter enzymes, however, can be obtained by grafting specific combinations of scaffoldin modules at the N-terminus of these cellulases, which are then used as cargo domains. ..
  43. Smith S, Bayer E. Insights into cellulosome assembly and dynamics: from dissection to reconstruction of the supramolecular enzyme complex. Curr Opin Struct Biol. 2013;23:686-94 pubmed publisher
    b>Cellulosomes are multi-enzyme complexes produced by anaerobic bacteria for the efficient deconstruction of plant cell wall polysaccharides...
  44. Eklund M, Sandström K, Teeri T, Nygren P. Site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex. J Biotechnol. 2004;109:277-86 pubmed
    ..The generality of this biospecificity-driven system for assembly applications is discussed. ..
  45. Leitão V, Noronha E, Camargo B, Hamann P, Steindorff A, Quirino B, et al. Growth and expression of relevant metabolic genes of Clostridium thermocellum cultured on lignocellulosic residues. J Ind Microbiol Biotechnol. 2017;44:825-834 pubmed publisher
    ..from goat rumen was characterized for its ability to grow on sugarcane straw and cotton waste, and to produce cellulosomes. We also evaluated C. thermocellum gene expression control in the presence of complex lignocellulosic biomasses...
  46. Dashtban M, Schraft H, Qin W. Fungal bioconversion of lignocellulosic residues; opportunities & perspectives. Int J Biol Sci. 2009;5:578-95 pubmed
  47. Moraïs S, Barak Y, Hadar Y, Wilson D, Shoham Y, Lamed R, et al. Assembly of xylanases into designer cellulosomes promotes efficient hydrolysis of the xylan component of a natural recalcitrant cellulosic substrate. MBio. 2011;2: pubmed publisher
    ..xylanases reflected their immediate juxtaposition in the complex, and these tetravalent xylanolytic designer cellulosomes succeeded in degrading significant (~25%) levels of the total xylan component of the wheat straw substrate...
  48. Xu C, Qin Y, Li Y, Ji Y, Huang J, Song H, et al. Factors influencing cellulosome activity in consolidated bioprocessing of cellulosic ethanol. Bioresour Technol. 2010;101:9560-9 pubmed publisher
    ..thermocellum monocultures. These findings demonstrate the potential advantages of bacterial cellulosome, and provide a novel strategy for design, selection and optimization of the cellulosome-ethanologen partnership. ..
  49. Hyeon J, Jeon W, Whang S, Han S. Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum. Enzyme Microb Technol. 2011;48:371-7 pubmed publisher
    ..glutamicum. The development of C. glutamicum strain that is capable of more effective cellulose hydrolysis brings about a realization of consolidated bioprocessing for the utilization of cellulosic biomass...
  50. Cunha E, Hatem C, Barrick D. Synergistic enhancement of cellulase pairs linked by consensus ankyrin repeats: Determination of the roles of spacing, orientation, and enzyme identity. Proteins. 2016;84:1043-54 pubmed publisher
    ..arrays can be used to vary spacing and orientation between enzymes, helping to design and optimize artificial cellulosomes, providing a novel architecture for synergistic enhancement of enzymatic cellulose degradation...
  51. Katyal P, Yang Y, Vinogradova O, Lin Y. Expression of Cellulolytic Enzyme as a Fusion Protein That Reacts Specifically With a Polymeric Scaffold. Methods Enzymol. 2017;590:259-276 pubmed publisher
    ..For example, cellulosomes are large complexes consisting of multiple cellulolytic enzymes that rely on the concerted actions of different ..
  52. Celik H, Blouzard J, Voigt B, Becher D, Trotter V, Fierobe H, et al. A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. PLoS ONE. 2013;8:e56063 pubmed publisher
    The composition of the cellulosomes (multi enzymatic complexes involved in the degradation of plant cell wall polysaccharides) produced by Clostridium cellulolyticum differs according to the growth substrate...
  53. Waeonukul R, Kosugi A, Prawitwong P, Deng L, Tachaapaikoon C, Pason P, et al. Novel cellulase recycling method using a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii ?-glucosidase. Bioresour Technol. 2013;130:424-30 pubmed publisher
    This report describes a novel recycling method utilizing a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii ?-glucosidase (CglT)...
  54. Suzuki H, Imaeda T, Kitagawa T, Kohda K. Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae. J Biotechnol. 2012;157:64-70 pubmed publisher
  55. Borne R, Bayer E, Pagès S, Perret S, Fierobe H. Unraveling enzyme discrimination during cellulosome assembly independent of cohesin-dockerin affinity. FEBS J. 2013;280:5764-79 pubmed publisher
    Bacterial cellulosomes are generally believed to assemble at random, like those produced by Clostridium cellulolyticum...
  56. Moraïs S, Morag E, Barak Y, Goldman D, Hadar Y, Lamed R, et al. Deconstruction of lignocellulose into soluble sugars by native and designer cellulosomes. MBio. 2012;3: pubmed publisher
    ..The action of these designer cellulosomes on wheat straw was 33 to 42% as efficient as the natural cellulosomes of Clostridium thermocellum...
  57. Carvalho A, Pires V, Gloster T, Turkenburg J, Prates J, Ferreira L, et al. Insights into the structural determinants of cohesin-dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA. J Mol Biol. 2005;349:909-15 pubmed
    The plant cell wall degrading enzymes expressed by anaerobic microorganisms form large multienzyme complexes (cellulosomes)...
  58. Cho W, Jeon S, Shim H, Doi R, Han S. Cellulosomic profiling produced by Clostridium cellulovorans during growth on different carbon sources explored by the cohesin marker. J Biotechnol. 2010;145:233-9 pubmed publisher
    Clostridium cellulovorans produces large extracellular enzyme complex, called cellulosomes. The diversity of the cellulosomal enzymes, which are secreted by C...
  59. Madkour M, Mayer F. Structural organization of the intact bacterial cellulosome as revealed by electron microscopy. Cell Biol Int. 2003;27:831-6 pubmed
    ..done because former electron microscopic studies aimed at elucidation of the structure of polycellulosomes and cellulosomes were restricted by the fact that data on macromolecular details could only be derived from deformed or ..
  60. Brunecky R, Alahuhta M, Bomble Y, Xu Q, Baker J, Ding S, et al. Structure and function of the Clostridium thermocellum cellobiohydrolase A X1-module repeat: enhancement through stabilization of the CbhA complex. Acta Crystallogr D Biol Crystallogr. 2012;68:292-9 pubmed publisher
    ..Furthermore, the results show that whereas the X1 modules do not seem to act as cellulose disruptors, they do aid in the thermostability of the CbhA complex, effectively allowing it to deconstruct cellulose at a higher temperature...
  61. Waeonukul R, Kosugi A, Tachaapaikoon C, Pason P, Ratanakhanokchai K, Prawitwong P, et al. Efficient saccharification of ammonia soaked rice straw by combination of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii ?-glucosidase. Bioresour Technol. 2012;107:352-7 pubmed publisher
    Clostridium thermocellum is known to produce the cellulosomes with efficient plant cell wall degradation ability...
  62. Noach I, Lamed R, Xu Q, Rosenheck S, Shimon L, Bayer E, et al. Preliminary X-ray characterization and phasing of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus. Acta Crystallogr D Biol Crystallogr. 2003;59:1670-3 pubmed
    ..The structure of the orthorhombic crystal form was solved by molecular replacement using the coordinates of the trigonal form...
  63. Liang C, Xue Y, Ma Y. [Synergistic systems for biodegradations of lignocellulose in microorganisms: a review]. Sheng Wu Gong Cheng Xue Bao. 2010;26:1327-32 pubmed
    ..Besides, bacterial co-cultures provide another synergistic cellulolytic system. Engineered strains with modified metabolic network could facilitate consolidated bioprocess by increasing yields as well as reducing costs...
  64. Kim H, Lee E, Park J, Sim S, Lee J. Reversible and multi-cyclic protein-protein interaction in bacterial cellulosome-mimic system using rod-shaped viral nanostructure. J Biotechnol. 2016;221:101-6 pubmed publisher
    ..EGFP that was fused to dockerin as a proof-of-concept here can be switched to other functional proteins/peptides that need to be used in multi-cyclic operation. ..
  65. Garvey M, Klose H, Fischer R, Lambertz C, Commandeur U. Cellulases for biomass degradation: comparing recombinant cellulase expression platforms. Trends Biotechnol. 2013;31:581-93 pubmed publisher
    ..We discuss current strategies for producing cellulases, and evaluate the benefits and drawbacks in yield, stability, and activity of enzymes from each system, and the overall progress in the field. ..
  66. Zagorski N. Profile of Roy H. Doi. Proc Natl Acad Sci U S A. 2007;104:9555-7 pubmed
  67. Slutzki M, Barak Y, Reshef D, Schueler Furman O, Lamed R, Bayer E. Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions. Methods Enzymol. 2012;510:417-28 pubmed publisher
    ..In light of the above, we propose this method as a preferred alternative for the relative quantification of high-affinity protein interactions...
  68. Yaniv O, Frolow F, Levy Assraf M, Lamed R, Bayer E. Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides. Methods Enzymol. 2012;510:247-59 pubmed publisher
    ..CBM3s, which are numerous components of both free cellulases and cellulosomes, bind tightly to crystalline cellulose, and thus play a key role in cellulose degradation through their ..
  69. Tamaru Y, Miyake H, Kuroda K, Nakanishi A, Matsushima C, Doi R, et al. Comparison of the mesophilic cellulosome-producing Clostridium cellulovorans genome with other cellulosome-related clostridial genomes. Microb Biotechnol. 2011;4:64-73 pubmed publisher
    ..Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced cellulosome-producing Clostridium strains for industrial applications such as biofuel production...