cell surface extensions

Summary

Summary: Specialized structures of the cell that extend the cell membrane and project out from the cell surface.

Top Publications

  1. Petrie R, Doyle A, Yamada K. Random versus directionally persistent cell migration. Nat Rev Mol Cell Biol. 2009;10:538-49 pubmed publisher
  2. Saltel F, Daubon T, Juin A, Ganuza I, Veillat V, Genot E. Invadosomes: intriguing structures with promise. Eur J Cell Biol. 2011;90:100-7 pubmed publisher
    ..To this end, 3D analysis of invadosomes is now being actively developed in ex vivo and in vivo models to demonstrate their occurrence and establish their role in pathological and physiological processes...
  3. Parekh A, Ruppender N, Branch K, Sewell Loftin M, Lin J, Boyer P, et al. Sensing and modulation of invadopodia across a wide range of rigidities. Biophys J. 2011;100:573-582 pubmed publisher
    ..These data suggest that cells can sense a wide range of rigidities, up into the GPa range. Furthermore, there is an optimal rigidity range for invadopodia activity that may be limited by BM rigidity. ..
  4. Yamaguchi H, Oikawa T. Membrane lipids in invadopodia and podosomes: key structures for cancer invasion and metastasis. Oncotarget. 2010;1:320-8 pubmed
    ..This review will summarize recent progress on the molecular mechanisms of invadopodia/podosome formation, with strong emphasis on the roles of lipid rafts and phosphoinositides...
  5. Yamaguchi H, Takeo Y, Yoshida S, Kouchi Z, Nakamura Y, Fukami K. Lipid rafts and caveolin-1 are required for invadopodia formation and extracellular matrix degradation by human breast cancer cells. Cancer Res. 2009;69:8594-602 pubmed publisher
    ..These results indicate that invadopodia are the sites where enrichment and trafficking of lipid rafts occur and that Cav-1 is an essential regulator of MT1-MMP function and invadopodia-mediated breast cancer cell invasion...
  6. Eckert M, Lwin T, Chang A, Kim J, Danis E, Ohno Machado L, et al. Twist1-induced invadopodia formation promotes tumor metastasis. Cancer Cell. 2011;19:372-86 pubmed publisher
    ..Therefore, invadopodia-mediated matrix degradation is a key function of Twist1 in promoting tumor metastasis...
  7. Thompson O, Kleino I, Crimaldi L, Gimona M, Saksela K, Winder S. Dystroglycan, Tks5 and Src mediated assembly of podosomes in myoblasts. PLoS ONE. 2008;3:e3638 pubmed publisher
  8. Gianni D, Diaz B, Taulet N, Fowler B, Courtneidge S, Bokoch G. Novel p47(phox)-related organizers regulate localized NADPH oxidase 1 (Nox1) activity. Sci Signal. 2009;2:ra54 pubmed publisher
    ..We propose that Tks organizers represent previously unrecognized members of an organizer superfamily that link Nox to localized ROS formation...
  9. Diaz B, Shani G, Pass I, Anderson D, Quintavalle M, Courtneidge S. Tks5-dependent, nox-mediated generation of reactive oxygen species is necessary for invadopodia formation. Sci Signal. 2009;2:ra53 pubmed publisher
    ..We propose that Tks5 facilitates the production of ROS necessary for invadopodia formation, and that in turn ROS modulate Tks5 tyrosine phosphorylation in a positive feedback loop...

More Information

Publications68

  1. Zheng Y, Bai C, Wang X. Potential significance of telocytes in the pathogenesis of lung diseases. Expert Rev Respir Med. 2012;6:45-9 pubmed publisher
    ..All characteristics of telocytes in lung tissue indicate that telocytes may play a potential, but important, role in the pathogenesis of lung diseases...
  2. Sherer N, Mothes W. Cytonemes and tunneling nanotubules in cell-cell communication and viral pathogenesis. Trends Cell Biol. 2008;18:414-20 pubmed publisher
    ..We propose models for the biogenesis of both types of membrane bridges and describe how viruses use these structures for the purpose of cell-to-cell spread...
  3. Veranic P, Lokar M, Schütz G, Weghuber J, Wieser S, Hagerstrand H, et al. Different types of cell-to-cell connections mediated by nanotubular structures. Biophys J. 2008;95:4416-25 pubmed publisher
    ..A possible model of formation and mechanical stability of nanotubes that bridge two neighboring cells is discussed...
  4. Crimaldi L, Courtneidge S, Gimona M. Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation. Exp Cell Res. 2009;315:2581-92 pubmed publisher
    ..Together our findings demonstrate that Tks5 plays a central role in the recruitment of AFAP-110, p190RhoGAP, and cortactin to drive podosome formation...
  5. Shimada A, Takano K, Shirouzu M, Hanawa Suetsugu K, Terada T, Toyooka K, et al. Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II. FEBS Lett. 2010;584:1111-8 pubmed publisher
    ..Since the curvature of the neck of the microspike and that of the tubulation share similar geometry, the pacsin2 EFC/F-BAR domain is considered to facilitate both microspike formation and tubulation...
  6. Stylli S, Stacey T, Verhagen A, Xu S, Pass I, Courtneidge S, et al. Nck adaptor proteins link Tks5 to invadopodia actin regulation and ECM degradation. J Cell Sci. 2009;122:2727-40 pubmed publisher
    ..These results identify a Src-Tks5-Nck pathway in ECM-degrading invadopodia that shows parallels with pathways linking several mammalian and pathogen-derived proteins to local actin regulation...
  7. Schoumacher M, Goldman R, Louvard D, Vignjevic D. Actin, microtubules, and vimentin intermediate filaments cooperate for elongation of invadopodia. J Cell Biol. 2010;189:541-56 pubmed publisher
    ..These findings provide a link between the epithelial to mesenchymal transition and BM transmigration...
  8. Rey M, Irondelle M, Waharte F, Lizarraga F, Chavrier P. HDAC6 is required for invadopodia activity and invasion by breast tumor cells. Eur J Cell Biol. 2011;90:128-35 pubmed publisher
    ..These data identify HDAC6 as a critical component of the invasive apparatus of tumor cells, in both two- and three-dimensional matrices...
  9. Bravo Cordero J, Oser M, Chen X, Eddy R, Hodgson L, Condeelis J. A novel spatiotemporal RhoC activation pathway locally regulates cofilin activity at invadopodia. Curr Biol. 2011;21:635-44 pubmed publisher
    ..However, it is not known which Rho GTPase is involved in regulating cofilin's phosphorylation status at invadopodia...
  10. Abmayr S, Pavlath G. Myoblast fusion: lessons from flies and mice. Development. 2012;139:641-56 pubmed publisher
    ..Here, we provide an overview of myoblast fusion in three model systems that have contributed much to our understanding of these events: the Drosophila embryo; developing and regenerating mouse muscle; and cultured rodent muscle cells...
  11. Ambrosi C, Pompili M, Scribano D, Zagaglia C, Ripa S, Nicoletti M. Outer membrane protein A (OmpA): a new player in shigella flexneri protrusion formation and inter-cellular spreading. PLoS ONE. 2012;7:e49625 pubmed publisher
    ..Moreover, an independent ompA mutant was generated. Since we found that both mutants displayed identical virulence profile, these results further supported the findings presented in this study...
  12. Sens K, Zhang S, Jin P, Duan R, Zhang G, Luo F, et al. An invasive podosome-like structure promotes fusion pore formation during myoblast fusion. J Cell Biol. 2010;191:1013-27 pubmed publisher
    ..Our studies uncover a novel invasive podosome-like structure (PLS) in a developing tissue and reveal a previously unrecognized function of PLSs in facilitating cell membrane juxtaposition and fusion...
  13. Sung B, WEAVER A. Cell-cell fusion: a new function for invadosomes. Curr Biol. 2011;21:R121-3 pubmed publisher
    ..Podosomes are cytoskeletal-based structures involved in extracellular matrix remodeling and cellular motility. A new study now implicates podosomes in pore formation during myoblast fusion...
  14. Nobile C, Rudnicka D, Hasan M, Aulner N, Porrot F, Machu C, et al. HIV-1 Nef inhibits ruffles, induces filopodia, and modulates migration of infected lymphocytes. J Virol. 2010;84:2282-93 pubmed publisher
    ..Therefore, Nef induces complex effects on the lymphocyte actin cytoskeleton and cellular morphology, which likely impacts the capacity of infected cells to circulate and to encounter and communicate with bystander cells...
  15. Dovas A, Cox D. Signaling networks regulating leukocyte podosome dynamics and function. Cell Signal. 2011;23:1225-34 pubmed publisher
    ..Here, we review the major signaling molecules that act in the formation and regulation of podosomes...
  16. Caneparo L, Pantazis P, Dempsey W, Fraser S. Intercellular bridges in vertebrate gastrulation. PLoS ONE. 2011;6:e20230 pubmed publisher
  17. Linder S, Wiesner C, Himmel M. Degrading devices: invadosomes in proteolytic cell invasion. Annu Rev Cell Dev Biol. 2011;27:185-211 pubmed publisher
  18. Pignatelli J, Tumbarello D, Schmidt R, Turner C. Hic-5 promotes invadopodia formation and invasion during TGF-?-induced epithelial-mesenchymal transition. J Cell Biol. 2012;197:421-37 pubmed publisher
    ..These data identify Hic-5 as a critical mediator of TGF-?-stimulated invadopodia formation, cell migration, and invasion...
  19. Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B, et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009;461:104-8 pubmed publisher
    ..A PA-Rac crystal structure and modelling revealed LOV-Rac interactions that will facilitate extension of this photoactivation approach to other proteins...
  20. Tolde O, Rosel D, Vesely P, Folk P, Brabek J. The structure of invadopodia in a complex 3D environment. Eur J Cell Biol. 2010;89:674-80 pubmed publisher
  21. Quintavalle M, Elia L, Condorelli G, Courtneidge S. MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro. J Cell Biol. 2010;189:13-22 pubmed publisher
    ..Thus, dysregulation of the miR-143 and -145 genes is causally involved in the aberrant SMC plasticity encountered during vascular disease, in part through the up-regulation of an autoregulatory loop that promotes podosome formation...
  22. Arkwright P, Luchetti F, Tour J, Roberts C, Ayub R, Morales A, et al. Fas stimulation of T lymphocytes promotes rapid intercellular exchange of death signals via membrane nanotubes. Cell Res. 2010;20:72-88 pubmed publisher
    ..We conclude that nanotube-mediated exchanges constitute a novel form of intercellular communication that augments the propagation of death signalling between neighbouring T cells...
  23. Murphy D, Courtneidge S. The 'ins' and 'outs' of podosomes and invadopodia: characteristics, formation and function. Nat Rev Mol Cell Biol. 2011;12:413-26 pubmed publisher
    ..Recently, progress has been made in our understanding of the regulatory and functional aspects of podosome and invadopodium biology and their role in human disease...
  24. Zheng Y, Bai C, Wang X. Telocyte morphologies and potential roles in diseases. J Cell Physiol. 2012;227:2311-7 pubmed publisher
    ..Future efforts are required to clarify pathophysiological functions of TCs in the disease...
  25. Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, et al. Coordination of Rho GTPase activities during cell protrusion. Nature. 2009;461:99-103 pubmed publisher
  26. Chan K, Cortesio C, Huttenlocher A. FAK alters invadopodia and focal adhesion composition and dynamics to regulate breast cancer invasion. J Cell Biol. 2009;185:357-70 pubmed publisher
    ..Collectively, our findings provide novel insight into the interplay between FAK and Src to promote invasion...
  27. Hamadi A, Deramaudt T, Takeda K, Ronde P. Src activation and translocation from focal adhesions to membrane ruffles contribute to formation of new adhesion sites. Cell Mol Life Sci. 2009;66:324-38 pubmed publisher
    ..We conclude that upon phosphorylation, Src is directly translocated from focal adhesions to membrane ruffles, thereby promoting formation of new adhesion complexes...
  28. Alexander N, Branch K, Parekh A, Clark E, Iwueke I, Guelcher S, et al. Extracellular matrix rigidity promotes invadopodia activity. Curr Biol. 2008;18:1295-1299 pubmed publisher
    ..These data suggest a potential mechanism, via invadopodia, for the reported correlation of tissue density with cancer aggressiveness...
  29. Hsu R, Tsai M, Hsieh Y, Lyu P, Yu J. Identification of MYO18A as a novel interacting partner of the PAK2/betaPIX/GIT1 complex and its potential function in modulating epithelial cell migration. Mol Biol Cell. 2010;21:287-301 pubmed publisher
  30. Caldieri G, Buccione R. Aiming for invadopodia: organizing polarized delivery at sites of invasion. Trends Cell Biol. 2010;20:64-70 pubmed publisher
    ..On this basis we hypothesize that the correct delivery of cargo to invadopodia is ensured by a polarized, cholesterol-dependent trafficking mechanism, similar to that of the apical domain of epithelial cells...
  31. Van Goethem E, Poincloux R, Gauffre F, Maridonneau Parini I, Le Cabec V. Matrix architecture dictates three-dimensional migration modes of human macrophages: differential involvement of proteases and podosome-like structures. J Immunol. 2010;184:1049-61 pubmed publisher
  32. Gianni D, Taulet N, DerMardirossian C, Bokoch G. c-Src-mediated phosphorylation of NoxA1 and Tks4 induces the reactive oxygen species (ROS)-dependent formation of functional invadopodia in human colon cancer cells. Mol Biol Cell. 2010;21:4287-98 pubmed publisher
    ..Taken together, these results elucidate the role of c-Src activity on the formation of invadopodia and may provide insight into the mechanisms of tumor formation in colon cancers...
  33. Van Goethem E, Guiet R, Balor S, Charriere G, Poincloux R, Labrousse A, et al. Macrophage podosomes go 3D. Eur J Cell Biol. 2011;90:224-36 pubmed publisher
    ..This work sets the scene for future studies of molecular and cellular processes regulating macrophage trans-migration...
  34. Nicolescu M, Popescu L. Telocytes in the interstitium of human exocrine pancreas: ultrastructural evidence. Pancreas. 2012;41:949-56 pubmed publisher
    ..Telocytes were initially described as interstitial Cajal-like cells, but it gradually became clear that they were a distinct novel cell type not directly related to canonical interstitial Cajal cells...
  35. Artym V, Matsumoto K, Mueller S, Yamada K. Dynamic membrane remodeling at invadopodia differentiates invadopodia from podosomes. Eur J Cell Biol. 2011;90:172-80 pubmed publisher
  36. Mattila P, Pykäläinen A, Saarikangas J, Paavilainen V, Vihinen H, Jokitalo E, et al. Missing-in-metastasis and IRSp53 deform PI(4,5)P2-rich membranes by an inverse BAR domain-like mechanism. J Cell Biol. 2007;176:953-64 pubmed
    ..Together, these data reveal that interplay between actin dynamics and a novel membrane-deformation activity promotes cell motility and morphogenesis. ..
  37. Wernimont S, Cortesio C, Simonson W, Huttenlocher A. Adhesions ring: a structural comparison between podosomes and the immune synapse. Eur J Cell Biol. 2008;87:507-15 pubmed publisher
    ..In this review, we compare the structures, functions and regulation of podosomes and the immune synapse. ..
  38. Chinnery H, Pearlman E, McMenamin P. Cutting edge: Membrane nanotubes in vivo: a feature of MHC class II+ cells in the mouse cornea. J Immunol. 2008;180:5779-83 pubmed
    ..Identification of these novel cellular processes in the mammalian cornea provides the first evidence of membrane nanotubes in vivo. ..
  39. Courtneidge S, Azucena E, Pass I, Seals D, Tesfay L. The SRC substrate Tks5, podosomes (invadopodia), and cancer cell invasion. Cold Spring Harb Symp Quant Biol. 2005;70:167-71 pubmed
    ..Our future work will focus on the identification of the binding partners of Tks5 that are responsible for podosome formation and invasion, and on determining the role of Tks5 in animal models of metastasis. ..
  40. Steffen A, Le Dez G, Poincloux R, Recchi C, Nassoy P, Rottner K, et al. MT1-MMP-dependent invasion is regulated by TI-VAMP/VAMP7. Curr Biol. 2008;18:926-31 pubmed publisher
    ..Collectively, these data point to a specific role of VAMP7 in delivering MT1-MMP to sites of degradation, maintaining the functional machinery required for invasion. ..
  41. van Helden S, Krooshoop D, Broers K, Raymakers R, Figdor C, van Leeuwen F. A critical role for prostaglandin E2 in podosome dissolution and induction of high-speed migration during dendritic cell maturation. J Immunol. 2006;177:1567-74 pubmed
    ..Together, these results show that maturation-induced podosome dissolution and loss of alpha(5)beta(1) integrin activity allow human DCs to undergo the transition from an adhesive to a highly migratory phenotype. ..
  42. Gringel A, Walz D, Rosenberger G, Minden A, Kutsche K, Kopp P, et al. PAK4 and alphaPIX determine podosome size and number in macrophages through localized actin regulation. J Cell Physiol. 2006;209:568-79 pubmed
    ..In sum, we propose that PAK4 and alphaPIX can induce highly localized changes in actin dynamics and thereby regulate size and number of podosomes in primary human macrophages...
  43. Scita G, Confalonieri S, Lappalainen P, Suetsugu S. IRSp53: crossing the road of membrane and actin dynamics in the formation of membrane protrusions. Trends Cell Biol. 2008;18:52-60 pubmed publisher
    ..Structural, biochemical and cell biological experiments support the unique role of this family as transducers of signalling, linking the protruding membrane to the underlying actin cytoskeleton...
  44. Kroboth K, Newton I, Kita K, Dikovskaya D, Zumbrunn J, Waterman Storer C, et al. Lack of adenomatous polyposis coli protein correlates with a decrease in cell migration and overall changes in microtubule stability. Mol Biol Cell. 2007;18:910-8 pubmed
    ..These data demonstrate that cell migration and microtubule stability are linked to APC status, thereby revealing a weakness in APC-deficient cells with potential therapeutic implications. ..
  45. Whipple R, Cheung A, Martin S. Detyrosinated microtubule protrusions in suspended mammary epithelial cells promote reattachment. Exp Cell Res. 2007;313:1326-36 pubmed
    ..Since bloodborne metastasis depends on both cell-cell and cell-matrix attachment, microtubule-based protrusions in detached mammary epithelial cells provide a novel mechanism that could influence the metastatic spread of breast tumors...
  46. Sarmiento C, Wang W, Dovas A, Yamaguchi H, Sidani M, El Sibai M, et al. WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells. J Cell Biol. 2008;180:1245-60 pubmed publisher
    ..These data show that coordinate regulation between the WASP family and mDia proteins controls the balance between lamellar and lamellipodial protrusion activity...
  47. Benninger Y, Thurnherr T, Pereira J, Krause S, Wu X, Chrostek Grashoff A, et al. Essential and distinct roles for cdc42 and rac1 in the regulation of Schwann cell biology during peripheral nervous system development. J Cell Biol. 2007;177:1051-61 pubmed
    ..Our results indicate that although cdc42 is required for normal Schwann cell proliferation, rac1 regulates Schwann cell process extension and stabilization, allowing efficient radial sorting of axon bundles...
  48. Baldassarre M, Ayala I, Beznoussenko G, Giacchetti G, Machesky L, Luini A, et al. Actin dynamics at sites of extracellular matrix degradation. Eur J Cell Biol. 2006;85:1217-31 pubmed
    ..In conclusion, invadopodia-associated actin comets are a striking example of consistently arising, spontaneous expression of actin-driven propulsion events that also represent a valuable experimental paradigm...
  49. Linder S. The matrix corroded: podosomes and invadopodia in extracellular matrix degradation. Trends Cell Biol. 2007;17:107-17 pubmed
    ..This review highlights important new developments in the field, discusses the common and divergent features of podosomes and invadopodia and summarizes current knowledge about matrix-degrading proteinases at these structures...
  50. Jeon T, Lee D, Merlot S, Weeks G, Firtel R. Rap1 controls cell adhesion and cell motility through the regulation of myosin II. J Cell Biol. 2007;176:1021-33 pubmed
    ..We suggest that Rap1/Phg2 plays a role in controlling leading edge myosin II disassembly while passively allowing myosin II assembly along the lateral sides and posterior of the cell...
  51. Fackler O, Grosse R. Cell motility through plasma membrane blebbing. J Cell Biol. 2008;181:879-84 pubmed publisher
    ..The importance of blebbing as a functional protrusion is underscored by the existence of multiple molecular mechanisms that govern actin-mediated bleb retraction...
  52. Nodari A, Zambroni D, Quattrini A, Court F, D Urso A, Recchia A, et al. Beta1 integrin activates Rac1 in Schwann cells to generate radial lamellae during axonal sorting and myelination. J Cell Biol. 2007;177:1063-75 pubmed
    ..Thus, increased activation of Rac1 by beta1 integrins allows Schwann cells to switch from migration/elongation to the extension of radial membranes required for axonal sorting and myelination...
  53. El Sibai M, Nalbant P, Pang H, Flinn R, Sarmiento C, Macaluso F, et al. Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells. J Cell Sci. 2007;120:3465-74 pubmed
    ..Our data suggest that Cdc42 activation is crucial for the regulation of actin polymerization in carcinoma cells, and required for both EGF-stimulated protrusion and cell motility independently of effects on Rac...
  54. Tournaviti S, Hannemann S, Terjung S, Kitzing T, Stegmayer C, Ritzerfeld J, et al. SH4-domain-induced plasma membrane dynamization promotes bleb-associated cell motility. J Cell Sci. 2007;120:3820-9 pubmed
  55. Sowinski S, Jolly C, Berninghausen O, Purbhoo M, Chauveau A, Köhler K, et al. Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nat Cell Biol. 2008;10:211-9 pubmed publisher
    ..These data lead us to propose that HIV-1 can spread using nanotubular connections formed by short-term intercellular unions in which T cells specialize...
  56. Ayala I, Baldassarre M, Giacchetti G, Caldieri G, Tete S, Luini A, et al. Multiple regulatory inputs converge on cortactin to control invadopodia biogenesis and extracellular matrix degradation. J Cell Sci. 2008;121:369-78 pubmed publisher
  57. Blouw B, Seals D, Pass I, Diaz B, Courtneidge S. A role for the podosome/invadopodia scaffold protein Tks5 in tumor growth in vivo. Eur J Cell Biol. 2008;87:555-67 pubmed publisher
    ..Our data potentially implicate a novel role of podosomes as mediators of tumor angiogenesis and support further exploration of how podosome formation and Tks5 expression contribute to tumor progression...
  58. Providence K, Higgins S, Mullen A, Battista A, Samarakoon R, Higgins C, et al. SERPINE1 (PAI-1) is deposited into keratinocyte migration "trails" and required for optimal monolayer wound repair. Arch Dermatol Res. 2008;300:303-10 pubmed publisher
  59. Stylli S, Kaye A, Lock P. Invadopodia: at the cutting edge of tumour invasion. J Clin Neurosci. 2008;15:725-37 pubmed publisher