megakaryocytes

Summary

Summary: Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.

Top Publications

  1. Bluteau D, Glembotsky A, Raimbault A, Balayn N, Gilles L, Rameau P, et al. Dysmegakaryopoiesis of FPD/AML pedigrees with constitutional RUNX1 mutations is linked to myosin II deregulated expression. Blood. 2012;120:2708-18 pubmed
    ..Here we studied the in vitro megakaryopoiesis of 3 FPD/AML pedigrees. A 60% to 80% decrease in the output of megakaryocytes (MKs) from CD34(+) was observed...
  2. Ono Y, Wang Y, Suzuki H, Okamoto S, Ikeda Y, Murata M, et al. Induction of functional platelets from mouse and human fibroblasts by p45NF-E2/Maf. Blood. 2012;120:3812-21 pubmed publisher
    Determinant factors leading from stem cells to megakaryocytes (MKs) and subsequently platelets have yet to be identified...
  3. Monzen S, Takahashi K, Yoshino H, Kasai Eguchi K, Kashiwakura I. Terminal maturation of megakaryocytes and platelet production by hematopoietic stem cells irradiated with heavy-ion beams. Radiat Res. 2011;176:8-16 pubmed
    ..This study examined the terminal maturation of megakaryocytes and platelet production derived from hematopoietic stem cells irradiated with heavy-ion beams...
  4. Zhang L, Orban M, Lorenz M, Barocke V, Braun D, Urtz N, et al. A novel role of sphingosine 1-phosphate receptor S1pr1 in mouse thrombopoiesis. J Exp Med. 2012;209:2165-81 pubmed publisher
    Millions of platelets are produced each hour by bone marrow (BM) megakaryocytes (MKs). MKs extend transendothelial proplatelet (PP) extensions into BM sinusoids and shed new platelets into the blood...
  5. Cornejo M, Mabialah V, Sykes S, Khandan T, Lo Celso C, Lopez C, et al. Crosstalk between NOTCH and AKT signaling during murine megakaryocyte lineage specification. Blood. 2011;118:1264-73 pubmed publisher
  6. Gerrits A, Gitz E, Koekman C, Visseren F, van Haeften T, Akkerman J. Induction of insulin resistance by the adipokines resistin, leptin, plasminogen activator inhibitor-1 and retinol binding protein 4 in human megakaryocytes. Haematologica. 2012;97:1149-57 pubmed publisher
    ..Platelets, the megakaryocytic cell line CHRF-288-11 and primary megakaryocytes were incubated with adipokines and with plasma from individuals with a disturbed adipokine profile...
  7. Borst O, Schmidt E, Münzer P, Schonberger T, Towhid S, Elvers M, et al. The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes. Blood. 2012;119:251-61 pubmed publisher
    ..for the first time, that the serum- and glucocorticoid-inducible kinase 1 (SGK1) is expressed in platelets and megakaryocytes. SOCE and agonist-induced [Ca2+](i) increase are significantly blunted in platelets from SGK1 knockout mice (..
  8. Monzen S, Tashiro E, Kashiwakura I. Megakaryocytopoiesis and thrombopoiesis in hematopoietic stem cells exposed to ionizing radiation. Radiat Res. 2011;176:716-24 pubmed
    ..This study examined the terminal maturation of megakaryocytes and platelet production in hematopoietic stem/progenitor cells (HSPCs) exposed to ionizing radiation...
  9. Takiguchi M, James C, Josefsson E, Carmichael C, Premsrirut P, Lowe S, et al. Transgenic, inducible RNAi in megakaryocytes and platelets in mice. J Thromb Haemost. 2010;8:2751-6 pubmed publisher
    ..CMV-rtTA mice) display inducible reporter gene expression with unusual and near-complete efficiency in megakaryocytes and platelets...

More Information

Publications72

  1. Konieczna I, Panuganti S, Deluca T, Papoutsakis E, Eklund E, Miller W. Administration of nicotinamide does not increase platelet levels in mice. Blood Cells Mol Dis. 2013;50:171-6 pubmed publisher
    ..Nicotinamide has been shown to enhance endomitosis in megakaryocytes cultured in vitro, suggesting that it may be beneficial for the production of platelets in culture...
  2. Nürnberg S, Rendon A, Smethurst P, Paul D, Voss K, Thon J, et al. A GWAS sequence variant for platelet volume marks an alternative DNM3 promoter in megakaryocytes near a MEIS1 binding site. Blood. 2012;120:4859-68 pubmed publisher
    ..Platelets are formed in the bone marrow by megakaryocytes, which are derived from hematopoietic stem cells by a process mainly controlled by transcription factors...
  3. Matsunaga T, Fukai F, Kameda T, Shide K, Shimoda H, Torii E, et al. Potentiated activation of VLA-4 and VLA-5 accelerates proplatelet-like formation. Ann Hematol. 2012;91:1633-43 pubmed publisher
    ..substantial role of FN receptors and their functional assignment in proplatelet-like formation (PPF) of megakaryocytes are not yet fully understood...
  4. Patel Hett S, Wang H, Begonja A, Thon J, Alden E, Wandersee N, et al. The spectrin-based membrane skeleton stabilizes mouse megakaryocyte membrane systems and is essential for proplatelet and platelet formation. Blood. 2011;118:1641-52 pubmed publisher
    b>Megakaryocytes generate platelets by remodeling their cytoplasm first into proplatelets and then into preplatelets, which undergo fission to generate platelets...
  5. Gao Y, Smith E, Ker E, Campbell P, Cheng E, Zou S, et al. Role of RhoA-specific guanine exchange factors in regulation of endomitosis in megakaryocytes. Dev Cell. 2012;22:573-84 pubmed publisher
    Polyploidization can precede the development of aneuploidy in cancer. Polyploidization in megakaryocytes (Mks), in contrast, is a highly controlled developmental process critical for efficient platelet production via unknown mechanisms...
  6. Wang J, Chen S, Zhang C, Stegeman S, Pfaff Amesse T, Zhang Y, et al. Human endometrial stromal stem cells differentiate into megakaryocytes with the ability to produce functional platelets. PLoS ONE. 2012;7:e44300 pubmed publisher
    ..The hESSCs have been differentiated into a number of cell lineages. However, differentiation of hESSCs into megakaryocytes (MKs) has not yet been investigated...
  7. White M, Schoenwaelder S, Josefsson E, Jarman K, Henley K, James C, et al. Caspase-9 mediates the apoptotic death of megakaryocytes and platelets, but is dispensable for their generation and function. Blood. 2012;119:4283-90 pubmed publisher
    Apoptotic caspases, including caspase-9, are thought to facilitate platelet shedding by megakaryocytes. They are known to be activated during platelet apoptosis, and have also been implicated in platelet hemostatic responses...
  8. Desterke C, Bilhou Nabera C, Guerton B, Martinaud C, Tonetti C, Clay D, et al. FLT3-mediated p38-MAPK activation participates in the control of megakaryopoiesis in primary myelofibrosis. Cancer Res. 2011;71:2901-15 pubmed publisher
  9. Tijssen M, Cvejic A, Joshi A, Hannah R, Ferreira R, Forrai A, et al. Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators. Dev Cell. 2011;20:597-609 pubmed publisher
    ..the five key hematopoietic transcription factors--GATA1, GATA2, RUNX1, FLI1, and TAL1/SCL--in primary human megakaryocytes. Statistical analysis of the 17,263 regions bound by at least one factor demonstrated that simultaneous ..
  10. Shabrani N, Khan N, Kale V, Limaye L. Polyunsaturated fatty acids confer cryoresistance on megakaryocytes generated from cord blood and also enhance megakaryocyte production from cryopreserved cord blood cells. Cytotherapy. 2012;14:366-80 pubmed publisher
    ..acid (DHA)/arachidonic acid (AA) has a beneficial effect on cytokine-mediated in vitro generation of megakaryocytes (MK) from umbilical cord blood (UCB)...
  11. Ono M, Matsubara Y, Shibano T, Ikeda Y, Murata M. GSK-3? negatively regulates megakaryocyte differentiation and platelet production from primary human bone marrow cells in vitro. Platelets. 2011;22:196-203 pubmed publisher
    ..These observations suggest that GSK-3? inhibition and TPO treatment affect both megakaryopoiesis and thrombopoiesis in an in vitro differentiation system for primary human bone marrow cells...
  12. Mazharian A, Wang Y, Mori J, Bem D, Finney B, Heising S, et al. Mice lacking the ITIM-containing receptor G6b-B exhibit macrothrombocytopenia and aberrant platelet function. Sci Signal. 2012;5:ra78 pubmed publisher
    ..Paradoxically, megakaryocytes, which produce platelets in the bone marrow, remain relatively refractory to the ECM-rich environment of the ..
  13. Beaulieu L, Lin E, Morin K, Tanriverdi K, Freedman J. Regulatory effects of TLR2 on megakaryocytic cell function. Blood. 2011;117:5963-74 pubmed publisher
    TLR2, a functional, inflammatory-related receptor, is known to be expressed on megakaryocytes and platelets and to lead to infection and immune-mediated activation of platelets; however, the role of this receptor in megakaryocytes is not ..
  14. Necchi V, Balduini A, Noris P, Barozzi S, Sommi P, di Buduo C, et al. Ubiquitin/proteasome-rich particulate cytoplasmic structures (PaCSs) in the platelets and megakaryocytes of ANKRD26-related thrombo-cytopenia. Thromb Haemost. 2013;109:263-71 pubmed publisher
    ..PaCSs were also extensively represented in ANKRD26-RT megakaryocytes, but not in healthy control megakaryocytes, and were absent in other ANKRD26-RT and control blood cells...
  15. Heazlewood S, Neaves R, Williams B, Haylock D, Adams T, Nilsson S. Megakaryocytes co-localise with hemopoietic stem cells and release cytokines that up-regulate stem cell proliferation. Stem Cell Res. 2013;11:782-92 pubmed publisher
    We report transplanted hemopoietic stem cells (HSC) preferentially lodge within two cells of mature megakaryocytes (MM). With both populations comprising ~0.2% of bone marrow cells, this strongly suggests a key functional interaction...
  16. Takayama N, Eto K. In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells. Methods Mol Biol. 2012;788:205-17 pubmed publisher
    ..We have optimized the in vitro culture condition for obtaining mature megakaryocytes derived from the hematopoietic progenitors within ES-sacs, which are then able to release platelets...
  17. Gieger C, Radhakrishnan A, Cvejic A, Tang W, Porcu E, Pistis G, et al. New gene functions in megakaryopoiesis and platelet formation. Nature. 2011;480:201-8 pubmed publisher
    ..Taken together, our findings advance understanding of novel gene functions controlling fate-determining events during megakaryopoiesis and platelet formation, providing a new example of successful translation of GWAS to function. ..
  18. Lordier L, Bluteau D, Jalil A, Legrand C, Pan J, Rameau P, et al. RUNX1-induced silencing of non-muscle myosin heavy chain IIB contributes to megakaryocyte polyploidization. Nat Commun. 2012;3:717 pubmed publisher
    b>Megakaryocytes are unique mammalian cells that undergo polyploidization (endomitosis) during differentiation, leading to an increase in cell size and protein production that precedes platelet production...
  19. Bury L, Malara A, Gresele P, Balduini A. Outside-in signalling generated by a constitutively activated integrin ?IIb?3 impairs proplatelet formation in human megakaryocytes. PLoS ONE. 2012;7:e34449 pubmed publisher
    The interaction of megakaryocytes with matrix proteins of the osteoblastic and vascular niche is essential for megakaryocyte maturation and proplatelet formation...
  20. Pleines I, Hagedorn I, Gupta S, May F, Chakarova L, van Hengel J, et al. Megakaryocyte-specific RhoA deficiency causes macrothrombocytopenia and defective platelet activation in hemostasis and thrombosis. Blood. 2012;119:1054-63 pubmed publisher
    ..Together, these results establish RhoA as an important regulator of platelet function in thrombosis and hemostasis...
  21. Dore L, Crispino J. Transcription factor networks in erythroid cell and megakaryocyte development. Blood. 2011;118:231-9 pubmed publisher
    Erythroid cells and megakaryocytes are derived from a common precursor, the megakaryocyte-erythroid progenitor...
  22. Di Michele M, Peeters K, Loyen S, Thys C, Waelkens E, Overbergh L, et al. Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) impairs the regulation of apoptosis in megakaryocytes by activating NF-?B: a proteomic study. Mol Cell Proteomics. 2012;11:M111.007625 pubmed publisher
    ..approach was here used to elucidate the molecular mechanisms underlying PACAP signaling via VPAC1 in megakaryocytes. Two-dimensional difference gel electrophoresis and tandem MS were applied to detect differentially expressed ..
  23. Thon J, Devine M, Jurak Begonja A, Tibbitts J, Italiano J. High-content live-cell imaging assay used to establish mechanism of trastuzumab emtansine (T-DM1)--mediated inhibition of platelet production. Blood. 2012;120:1975-84 pubmed publisher
    ..High-content analysis of primary cell cultures revealed that T-DM1 is taken up by mouse megakaryocytes, inhibits megakaryocyte differentiation, and disrupts proplatelet formation by inducing abnormal tubulin ..
  24. Malara A, Gruppi C, Pallotta I, Spedden E, Tenni R, Raspanti M, et al. Extracellular matrix structure and nano-mechanics determine megakaryocyte function. Blood. 2011;118:4449-53 pubmed publisher
    ..On the contrary, a decrease in mechanical tension caused by N-acetylation of lysine side chains in type I collagen completely reverted these processes by preventing fibrillogenesis...
  25. Munoz Alonso M, Ceballos L, Bretones G, Frade P, Leon J, Gandarillas A. MYC accelerates p21CIP-induced megakaryocytic differentiation involving early mitosis arrest in leukemia cells. J Cell Physiol. 2012;227:2069-78 pubmed publisher
    ..Our data suggests that in some systems p21(CIP) takes part in a mitosis control driving MYC-induced cellular growth into differentiation...
  26. Truman L, Bentley K, SMITH E, Massaro S, Gonzalez D, Haberman A, et al. ProxTom lymphatic vessel reporter mice reveal Prox1 expression in the adrenal medulla, megakaryocytes, and platelets. Am J Pathol. 2012;180:1715-25 pubmed publisher
    ..mice revealed the expression of Prox1 in three novel sites: the neuroendocrine cells of the adrenal medulla, megakaryocytes, and platelets. The novel sites identified herein suggest previously unknown roles for Prox1...
  27. Chagraoui H, Kassouf M, Banerjee S, Goardon N, Clark K, Atzberger A, et al. SCL-mediated regulation of the cell-cycle regulator p21 is critical for murine megakaryopoiesis. Blood. 2011;118:723-35 pubmed publisher
    ..Our study provides a mechanistic link between a major hematopoietic transcriptional regulator, cell-cycle progression, and megakaryocytic differentiation...
  28. Josefsson E, James C, Henley K, Debrincat M, Rogers K, Dowling M, et al. Megakaryocytes possess a functional intrinsic apoptosis pathway that must be restrained to survive and produce platelets. J Exp Med. 2011;208:2017-31 pubmed publisher
    It is believed that megakaryocytes undergo a specialized form of apoptosis to shed platelets...
  29. Lev P, Goette N, Glembotsky A, Laguens R, Meckert P, Salim J, et al. Production of functional platelet-like particles by the megakaryoblastic DAMI cell line provides a model for platelet biogenesis. Platelets. 2011;22:28-38 pubmed publisher
    ..DAMI cell line induced to differentiate into mature megakaryocytes is able to produce functional platelets providing a suitable model to study the mechanisms involved in ..
  30. Mazharian A, Ghevaert C, Zhang L, Massberg S, Watson S. Dasatinib enhances megakaryocyte differentiation but inhibits platelet formation. Blood. 2011;117:5198-206 pubmed publisher
    ..Conversely, the number of megakaryocytes (MKs) in the bone marrow of dasatinib-treated mice was increased and the ploidy of MKs derived from bone ..
  31. Mercado C, Byrum S, Beggs M, Ziu E, Singh P, Raj V, et al. Impact of elevated plasma serotonin on global gene expression of murine megakaryocytes. PLoS ONE. 2013;8:e72580 pubmed publisher
    ..Today, neither the effective modulators on 5-HT signaling pathways nor the genes affected by 5-HT signal are well known yet...
  32. Naziroglu M. TRPM2 channel membrane currents in primary rat megakaryocytes were activated by the agonist ADP-ribose but not oxidative stress. J Membr Biol. 2011;241:51-7 pubmed publisher
    ..We tested the effects of FFA and 2-APB on ADPR-induced TRPM2 cation channel currents in rat native bone marrow megakaryocytes. Megakaryocyte cells were freshly isolated from rat bone marrow and studied with the conventional whole-cell ..
  33. Pleines I, Dütting S, Cherpokova D, Eckly A, Meyer I, Morowski M, et al. Defective tubulin organization and proplatelet formation in murine megakaryocytes lacking Rac1 and Cdc42. Blood. 2013;122:3178-87 pubmed publisher
    ..Platelets are produced by bone marrow megakaryocytes (MKs), which extend protrusions, or so-called proplatelets, into bone marrow sinusoids...
  34. Schachtner H, Calaminus S, Sinclair A, Monypenny J, Blundell M, Leon C, et al. Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane. Blood. 2013;121:2542-52 pubmed publisher
    b>Megakaryocytes give rise to platelets via extension of proplatelet arms, which are released through the vascular sinusoids into the bloodstream...
  35. Habart D, Cheli Y, Nugent D, Ruggeri Z, Kunicki T. Conditional knockout of integrin ?2?1 in murine megakaryocytes leads to reduced mean platelet volume. PLoS ONE. 2013;8:e55094 pubmed publisher
    ..This transgenic mouse strain bearing a floxed Itga2 gene will prove valuable to distinguish in vivo the temporal and spatial contributions of ?2 integrin in selected cell types...
  36. Deutsch V, Tomer A. Advances in megakaryocytopoiesis and thrombopoiesis: from bench to bedside. Br J Haematol. 2013;161:778-93 pubmed publisher
    ..stem cells, proliferation and terminal differentiation of megakaryocytic progenitors (MK-p) and maturation of megakaryocytes (MKs) to produce functional platelets...
  37. Lordier L, Pan J, Naim V, Jalil A, Badirou I, Rameau P, et al. Presence of a defect in karyokinesis during megakaryocyte endomitosis. Cell Cycle. 2012;11:4385-9 pubmed publisher
    ..Generally, a pure defect in cytokinesis produces a multinucleated cell, but megakaryocytes are characterized by a single polylobulated nucleus with a 2 (N) ploidy...
  38. Wang Y, Meng R, Hayes V, Fuentes R, Yu X, Abrams C, et al. Pleiotropic platelet defects in mice with disrupted FOG1-NuRD interaction. Blood. 2011;118:6183-91 pubmed publisher
    ..We found markedly deficient levels of P-selectin protein limited to megakaryocytes and platelets...
  39. Kacena M, Eleniste P, Cheng Y, Huang S, Shivanna M, Meijome T, et al. Megakaryocytes regulate expression of Pyk2 isoforms and caspase-mediated cleavage of actin in osteoblasts. J Biol Chem. 2012;287:17257-68 pubmed publisher
    ..We reported that OB proliferation is enhanced by megakaryocytes (MKs) via a process that is regulated in part by integrin signaling...
  40. Leysi Derilou Y, Duchesne C, Garnier A, Pineault N. Single-cell level analysis of megakaryocyte growth and development. Differentiation. 2012;83:200-9 pubmed publisher
    ..Conversely, only NIC treatment increased the endomitotic rate of megakaryocytes (MKs) leading to the formation of CB-MKs with ploidy level frequently observed with BM-MKs...
  41. Nakagawa Y, Nakamura S, Nakajima M, Endo H, Dohda T, Takayama N, et al. Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes. Exp Hematol. 2013;41:742-8 pubmed publisher
    ..However, the efficiency of in vitro platelet generation from megakaryocytes (MKs) under static culture conditions is lower than is seen in vivo...
  42. Zhang Y, Conti M, Malide D, Dong F, Wang A, Shmist Y, et al. Mouse models of MYH9-related disease: mutations in nonmuscle myosin II-A. Blood. 2012;119:238-50 pubmed publisher
    ..macrothrombocytopenia with prolonged bleeding times, a defect in clot retraction, and increased extramedullary megakaryocytes. Studies of cultured megakaryocytes and live-cell imaging of megakaryocytes in the BM show that heterozygous ..
  43. Olson T, Caselli A, Otsuru S, Hofmann T, Williams R, Paolucci P, et al. Megakaryocytes promote murine osteoblastic HSC niche expansion and stem cell engraftment after radioablative conditioning. Blood. 2013;121:5238-49 pubmed publisher
    ..endosteal osteoblastic HSC niche after total body irradiation (TBI), defined as relocalization of surviving megakaryocytes to the niche site and marked expansion of endosteal osteoblasts...
  44. Shin J, Swift J, Spinler K, Discher D. Myosin-II inhibition and soft 2D matrix maximize multinucleation and cellular projections typical of platelet-producing megakaryocytes. Proc Natl Acad Sci U S A. 2011;108:11458-63 pubmed publisher
    ..a rigid matrix are all promoted by myosin-II, and so multinucleated cells with distended membranes--typical of megakaryocytes (MKs)--seem predictable for low myosin activity in cells on soft matrices...
  45. Geddis A. Congenital amegakaryocytic thrombocytopenia. Pediatr Blood Cancer. 2011;57:199-203 pubmed publisher
    ..presenting at birth in a child without congenital or skeletal malformations, reduced or absent bone marrow megakaryocytes, and eventual progression to bone marrow failure...
  46. Thon J, Montalvo A, Patel Hett S, Devine M, Richardson J, Ehrlicher A, et al. Cytoskeletal mechanics of proplatelet maturation and platelet release. J Cell Biol. 2010;191:861-74 pubmed publisher
    b>Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production...
  47. Lu S, Li F, Yin H, Feng Q, Kimbrel E, Hahm E, et al. Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice. Cell Res. 2011;21:530-45 pubmed publisher
    ..In this study, we report that it is feasible to generate functional megakaryocytes and platelets from human embryonic stem cells (hESCs) on a large scale...
  48. Malara A, Gruppi C, Rebuzzini P, Visai L, Perotti C, Moratti R, et al. Megakaryocyte-matrix interaction within bone marrow: new roles for fibronectin and factor XIII-A. Blood. 2011;117:2476-83 pubmed publisher
    The mechanisms by which megakaryocytes (MKs) differentiate and release platelets into the circulation are not well understood...
  49. Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H, et al. Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells. J Exp Med. 2010;207:2817-30 pubmed publisher
    ..In contrast, sustained and excessive c-MYC expression in megakaryocytes was accompanied by increased p14 (ARF) and p16 (INK4A) expression, decreased GATA1 expression, and impaired ..
  50. Sullivan S, Mills J, Koukouritaki S, Vo K, Lyde R, Paluru P, et al. High-level transgene expression in induced pluripotent stem cell-derived megakaryocytes: correction of Glanzmann thrombasthenia. Blood. 2014;123:753-7 pubmed publisher
    ..pluripotent stem cells (iPSCs) offers a new approach to study and potentially treat disorders affecting megakaryocytes and platelets...
  51. Lee S, Kwon J, Cho S, Jarrar Y, Shin J. Effects of testosterone and 17?-oestradiol on expression of the G protein-coupled receptor P2Y12 in megakaryocytic DAMI cells. Platelets. 2012;23:579-85 pubmed publisher
    ..Further studies using primary human megakaryocytes and platelets could be necessary to know the effect of hormones on the P2Y12 expression in circulating ..
  52. Schipper L, Brand A, Fibbe W, van Hensbergen Y. Functional characterization of TPO-expanded CD34+ cord blood cells identifies CD34- CD61- cells as platelet-producing cells early after transplantation in NOD/SCID mice and rCD34+ cells as CAFC colony-forming cells. Stem Cells. 2012;30:988-96 pubmed publisher
    ..These data support that functional characterization of the expanded cell populations is warranted to make future expansion protocols suitable for clinical application...
  53. Yu M, Cantor A. Megakaryopoiesis and thrombopoiesis: an update on cytokines and lineage surface markers. Methods Mol Biol. 2012;788:291-303 pubmed publisher
    Megakaryopoiesis is the process by which mature megakaryocytes (MKs) develop from hematopoietic stem cells (HSCs)...
  54. Tolhurst G, Carter R, Miller N, Mahaut Smith M. Purification of native bone marrow megakaryocytes for studies of gene expression. Methods Mol Biol. 2012;788:259-73 pubmed publisher
    b>Megakaryocytes constitute less than 1% of all marrow cells, therefore purification of these giant platelet precursor cells represents a challenge...
  55. Thon J, Italiano J. Visualization and manipulation of the platelet and megakaryocyte cytoskeleton. Methods Mol Biol. 2012;788:109-25 pubmed publisher
    ..membrane system, delineated the mechanics of organelle transport and microtubule assembly in living megakaryocytes, described the process of platelet production in vivo, and revealed factors contributing to and the mechanisms ..
  56. Psaila B, Lyden D, Roberts I. Megakaryocytes, malignancy and bone marrow vascular niches. J Thromb Haemost. 2012;10:177-88 pubmed publisher
    ..The vascular niche is conducive for thrombopoiesis and megakaryocytes may, in turn, regulate the vascular niche, especially in supporting vascular and hematopoietic regeneration ..
  57. Hussein K. Gene expression profiling in laser-microdissected bone marrow megakaryocytes. Methods Mol Biol. 2011;755:429-39 pubmed publisher
    Gene expression analysis of the megakaryocytic lineage requires isolation of megakaryocytes from their bone marrow microenvironment...
  58. Balduini A, Badalucco S, Pugliano M, Baev D, De Silvestri A, Cattaneo M, et al. In vitro megakaryocyte differentiation and proplatelet formation in Ph-negative classical myeloproliferative neoplasms: distinct patterns in the different clinical phenotypes. PLoS ONE. 2011;6:e21015 pubmed publisher
    ..Although the pathogenesis of MPNs is still incompletely understood, an involvement of the megakaryocyte lineage is a distinctive feature...
  59. Jurak Begonja A, Hoffmeister K, Hartwig J, Falet H. FlnA-null megakaryocytes prematurely release large and fragile platelets that circulate poorly. Blood. 2011;118:2285-95 pubmed publisher
    ..Together, the findings indicate that FlnA-null MKs prematurely release large and fragile platelets that are removed rapidly from the circulation by macrophages. ..
  60. Kunishima S, Kashiwagi H, Otsu M, Takayama N, Eto K, Onodera M, et al. Heterozygous ITGA2B R995W mutation inducing constitutive activation of the ?IIb?3 receptor affects proplatelet formation and causes congenital macrothrombocytopenia. Blood. 2011;117:5479-84 pubmed publisher
    ..increased size and decreased number of proplatelet tips in ?IIb-W995/?3-transduced mouse fetal liver-derived megakaryocytes indicate defective pro-platelet formation...
  61. Liu Z, Italiano J, Ferrer Marin F, Gutti R, Bailey M, Poterjoy B, et al. Developmental differences in megakaryocytopoiesis are associated with up-regulated TPO signaling through mTOR and elevated GATA-1 levels in neonatal megakaryocytes. Blood. 2011;117:4106-17 pubmed publisher
  62. Matsubara Y, Murata M, Ikeda Y. Culture of megakaryocytes and platelets from subcutaneous adipose tissue and a preadipocyte cell line. Methods Mol Biol. 2012;788:249-58 pubmed publisher
    The molecular mechanisms whereby stem cells develop into platelet-producing megakaryocytes (MKs) are not yet fully understood...
  63. Kudo T, Sato T, Hagiwara K, Kozuma Y, Yamaguchi T, Ikehara Y, et al. C1galt1-deficient mice exhibit thrombocytopenia due to abnormal terminal differentiation of megakaryocytes. Blood. 2013;122:1649-57 pubmed publisher
    ..Both the number and DNA ploidy of megakaryocytes in Mx1-C1 bone marrow were similar to those in wild-type (WT) mice...