novobiocin

Summary

Summary: An antibiotic compound derived from Streptomyces niveus. It has a chemical structure similar to coumarin. Novobiocin binds to DNA gyrase, and blocks adenosine triphosphatase (ATPase) activity. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p189)

Top Publications

  1. Hardy C, Cozzarelli N. Alteration of Escherichia coli topoisomerase IV to novobiocin resistance. Antimicrob Agents Chemother. 2003;47:941-7 pubmed
    ..Coumarins, such as novobiocin, are wide-spectrum antimicrobial agents that primarily interfere with DNA gyrase...
  2. Holdgate G, Tunnicliffe A, Ward W, Weston S, Rosenbrock G, Barth P, et al. The entropic penalty of ordered water accounts for weaker binding of the antibiotic novobiocin to a resistant mutant of DNA gyrase: a thermodynamic and crystallographic study. Biochemistry. 1997;36:9663-73 pubmed publisher
    b>Novobiocin is an antibiotic which binds to a 24 kDa fragment from the B subunit of DNA gyrase. Naturally occurring resistance arises from mutation of Arg-136 which hydrogen bonds to the coumarin ring of novobiocin...
  3. Allan R, Mok D, Ward B, Ratajczak T. Modulation of chaperone function and cochaperone interaction by novobiocin in the C-terminal domain of Hsp90: evidence that coumarin antibiotics disrupt Hsp90 dimerization. J Biol Chem. 2006;281:7161-71 pubmed
    ..contributes to the Hsp90 interaction with the immunophilin cochaperones and corresponds to the binding site for novobiocin, a coumarin-related Hsp90 inhibitor...
  4. Shiozawa K, Oka M, Soda H, Yoshikawa M, Ikegami Y, Tsurutani J, et al. Reversal of breast cancer resistance protein (BCRP/ABCG2)-mediated drug resistance by novobiocin, a coumermycin antibiotic. Int J Cancer. 2004;108:146-51 pubmed
    ..b>Novobiocin, a coumermycin antibiotic, is known to enhance anticancer drug sensitivity of cancer cells in vitro and in vivo, ..
  5. Matthews S, Vielhauer G, Manthe C, Chaguturu V, Szabla K, Matts R, et al. Characterization of a novel novobiocin analogue as a putative C-terminal inhibitor of heat shock protein 90 in prostate cancer cells. Prostate. 2010;70:27-36 pubmed publisher
    ..Previously, we reported the development of novobiocin analogues designed to inhibit the C-terminal portion of Hsp90, which demonstrated the ability to decrease client ..
  6. Gross C, Parsons J, Grossman T, Charifson P, Bellon S, Jernee J, et al. Active-site residues of Escherichia coli DNA gyrase required in coupling ATP hydrolysis to DNA supercoiling and amino acid substitutions leading to novobiocin resistance. Antimicrob Agents Chemother. 2003;47:1037-46 pubmed
    ..Amino acids in proximity to bound nonhydrolyzable ATP analog (AMP. PNP) or novobiocin in the gyrase B (GyrB) subunit crystal structures were examined for their roles in enzyme function and ..
  7. Conde R, Belak Z, Nair M, O Carroll R, Ovsenek N. Modulation of Hsf1 activity by novobiocin and geldanamycin. Biochem Cell Biol. 2009;87:845-51 pubmed publisher
    ..Hsp90 is a known modulator of HSF1 activity, we examined the effects of two pharmacological inhibitors of Hsp90, novobiocin and geldanamycin, on HSF1 DNA-binding activity in the Xenopus oocyte model system...
  8. Zhao H, Blagg B. Novobiocin analogues with second-generation noviose surrogates. Bioorg Med Chem Lett. 2013;23:552-7 pubmed publisher
    Hsp90 is a promising therapeutic target for the treatment of cancer. Novobiocin is the first Hsp90 C-terminal inhibitor ever identified and recent structure-activity relationship studies on the noviose sugar identified several ..
  9. Donnelly A, Blagg B. Novobiocin and additional inhibitors of the Hsp90 C-terminal nucleotide-binding pocket. Curr Med Chem. 2008;15:2702-17 pubmed
    ..This C-terminal nucleotide binding pocket has been shown to not only bind ATP, but cisplatin, novobiocin, epilgallocatechin-3-gallate (EGCG) and taxol...

More Information

Publications62

  1. Pacholec M, Freel Meyers C, Oberthür M, Kahne D, Walsh C. Characterization of the aminocoumarin ligase SimL from the simocyclinone pathway and tandem incubation with NovM,P,N from the novobiocin pathway. Biochemistry. 2005;44:4949-56 pubmed
    ..Unlike the antibiotics novobiocin, clorobiocin, and coumermycin A(1), the phenolic hydroxyl group of the aminocoumarin in simocyclinone is not ..
  2. Lamour V, Hoermann L, Jeltsch J, Oudet P, Moras D. Crystallization of the 43 kDa ATPase domain of Thermus thermophilus gyrase B in complex with novobiocin. Acta Crystallogr D Biol Crystallogr. 2002;58:1376-8 pubmed
    ..Crystals of the 43 kDa domain in complex with novobiocin, one of the most potent inhibitors of bacterial topoisomerases, were obtained...
  3. Chen H, Walsh C. Coumarin formation in novobiocin biosynthesis: beta-hydroxylation of the aminoacyl enzyme tyrosyl-S-NovH by a cytochrome P450 NovI. Chem Biol. 2001;8:301-12 pubmed
    Coumarin group antibiotics, such as novobiocin, coumermycin A1 and clorobiocin, are potent inhibitors of DNA gyrase...
  4. Eustáquio A, Gust B, Galm U, Li S, Chater K, Heide L. Heterologous expression of novobiocin and clorobiocin biosynthetic gene clusters. Appl Environ Microbiol. 2005;71:2452-9 pubmed
    ..This method was used to express the gene clusters of the aminocoumarin antibiotics novobiocin and clorobiocin in the well-studied strains Streptomyces coelicolor and Streptomyces lividans, which, in ..
  5. Marcu M, Chadli A, Bouhouche I, Catelli M, Neckers L. The heat shock protein 90 antagonist novobiocin interacts with a previously unrecognized ATP-binding domain in the carboxyl terminus of the chaperone. J Biol Chem. 2000;275:37181-6 pubmed
    ..We recently demonstrated that the gyrase inhibitor novobiocin also interacts with Hsp90, altering the affinity of the chaperone for geldanamycin and radicicol and causing in ..
  6. Jovanovic M, Lilic M, Janjusevic R, Jovanovic G, Savic D, Milija J. tRNA synthetase mutants of Escherichia coli K-12 are resistant to the gyrase inhibitor novobiocin. J Bacteriol. 1999;181:2979-83 pubmed
    ..we demonstrated that mutations in the genes cysB, cysE, and cls (nov) affect resistance of Escherichia coli to novobiocin (J. Rakonjac, M. Milic, and D. J. Savic, Mol. Gen. Genet. 228:307-311, 1991; R. Ivanisevic, M. Milic, D...
  7. Burlison J, Neckers L, Smith A, Maxwell A, Blagg B. Novobiocin: redesigning a DNA gyrase inhibitor for selective inhibition of hsp90. J Am Chem Soc. 2006;128:15529-36 pubmed
    b>Novobiocin is a member of the coumermycin family of antibiotics and is a well-established inhibitor of DNA gyrase...
  8. Singh G, Jayanarayan K, Dey C. Novobiocin induces apoptosis-like cell death in topoisomerase II over-expressing arsenite resistant Leishmania donovani. Mol Biochem Parasitol. 2005;141:57-69 pubmed
    ..Here we report that the arsenite resistant strain of L. donovani promastigotes shows cross-resistance to novobiocin, a catalytic inhibitor of topoisomerase II, with IC50 value of 320 microg ml-1 as compared with 242 microg ml-1 ..
  9. Lu Y, Ansar S, Michaelis M, Blagg B. Neuroprotective activity and evaluation of Hsp90 inhibitors in an immortalized neuronal cell line. Bioorg Med Chem. 2009;17:1709-15 pubmed publisher
    ..and cytotoxicity resulting from exposure to Abeta peptides to evaluate the neuroprotective properties of novel novobiocin analogues and established Hsp90 inhibitors...
  10. Yu X, Shen G, Neckers L, Blake H, Holzbeierlein J, Cronk B, et al. Hsp90 inhibitors identified from a library of novobiocin analogues. J Am Chem Soc. 2005;127:12778-9 pubmed
    b>Novobiocin is a C-terminal inhibitor of the Hsp90 protein folding machinery, which is responsible for the conformational maturation of numerous proteins involved in cancer growth and survival...
  11. Huang Y, Blagg B. A library of noviosylated coumarin analogues. J Org Chem. 2007;72:3609-13 pubmed
    The DNA gyrase inhibitor, novobiocin, was recently shown to inhibit Hsp90 via a previously unrecognized C-terminal ATP-binding site...
  12. Vickers A, Chopra I, O Neill A. Intrinsic novobiocin resistance in Staphylococcus saprophyticus. Antimicrob Agents Chemother. 2007;51:4484-5 pubmed
    Intrinsic novobiocin resistance in Staphylococcus saprophyticus was associated with expression of a novobiocin-resistant form of the drug target protein (GyrB)...
  13. Le Bras G, Radanyi C, Peyrat J, Brion J, Alami M, Marsaud V, et al. New novobiocin analogues as antiproliferative agents in breast cancer cells and potential inhibitors of heat shock protein 90. J Med Chem. 2007;50:6189-200 pubmed
    ..Investigation of novobiocin analogues lacking the noviose moiety as novel inhibitors of hsp90 was carried out...
  14. Radanyi C, Le Bras G, Messaoudi S, Bouclier C, Peyrat J, Brion J, et al. Synthesis and biological activity of simplified denoviose-coumarins related to novobiocin as potent inhibitors of heat-shock protein 90 (hsp90). Bioorg Med Chem Lett. 2008;18:2495-8 pubmed publisher
    ..In cell proliferation assay, the most active compound 5g was approximately 8 times more potent than the parent novobiocin natural compound.
  15. Kerschmann R, Wolfson J, McHugh G, Dickersin G, Hooper D, Swartz M. Novobiocin-induced ultrastructural changes and antagonism of DNA synthesis in Trypanosoma cruzi amastigotes growing in cell-free medium. J Protozool. 1989;36:14-20 pubmed
    The antimicrobial agent novobiocin, an inhibitor of the bacterial enzyme topoisomerase II (DNA gyrase), is known to antagonize Trypanosoma cruzi amastigotes growing in cell-free medium...
  16. Wu L, Xu J, Zhang K, Lin Q, Huang X, Wen C, et al. Disruption of the Bcr-Abl/Hsp90 protein complex: a possible mechanism to inhibit Bcr-Abl-positive human leukemic blasts by novobiocin. Leukemia. 2008;22:1402-9 pubmed publisher
    ..Because Bcr-Abl TK is chaperoned by Hsp90 (90 kDa heat-shock protein), we investigated the effects of novobiocin (NB), an Hsp90 C-terminal inhibitor, on the viability of the Bcr-Abl-positive human leukemia cells HL-60/Bcr-Abl ..
  17. Pridgeon J, Klesius P. Development and efficacy of a novobiocin-resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus). Vaccine. 2011;29:5986-93 pubmed publisher
    ..iniae vaccine was developed from a virulent strain of Streptococcus iniae (ISET0901) through selection for novobiocin resistance (named ISNO)...
  18. Luo X, Zou J, Wang S, Zhang T, Xi T. Novobiocin decreases SMYD3 expression and inhibits the migration of MDA-MB-231 human breast cancer cells. IUBMB Life. 2010;62:194-9 pubmed publisher
    ..In this study, we further found that novobiocin, a HSP90 inhibitor, could decrease the expression of SMYD3 and dose dependently inhibit the proliferation and ..
  19. Steffensky M, Mühlenweg A, Wang Z, Li S, Heide L. Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891. Antimicrob Agents Chemother. 2000;44:1214-22 pubmed
    The novobiocin biosynthetic gene cluster from Streptomyces spheroides NCIB 11891 was cloned by using homologous deoxynucleoside diphosphate (dNDP)-glucose 4,6-dehydratase gene fragments as probes. Double-stranded sequencing of 25...
  20. Sanchez M, Watts J. Enhancement of the activity of novobiocin against Escherichia coli by lactoferrin. J Dairy Sci. 1999;82:494-9 pubmed
    The activity of novobiocin against Escherichia coli ATCC 25922 and three E. coli strains that were isolated from cases of bovine mastitis was determined in timekill studies in the presence of bovine lactoferrin...
  21. Zhang L, Zhao H, Blagg B, Dobrowsky R. C-terminal heat shock protein 90 inhibitor decreases hyperglycemia-induced oxidative stress and improves mitochondrial bioenergetics in sensory neurons. J Proteome Res. 2012;11:2581-93 pubmed publisher
    ..KU-32 is a novobiocin-based, C-terminal inhibitor of the molecular chaperone, heat shock protein 90 (Hsp90)...
  22. Kusuma B, Duerfeldt A, Blagg B. Synthesis and biological evaluation of arylated novobiocin analogs as Hsp90 inhibitors. Bioorg Med Chem Lett. 2011;21:7170-4 pubmed publisher
    b>Novobiocin analogs lacking labile glycosidic ether have been designed, synthesized and evaluated for Hsp90 inhibitory activity...
  23. Jakimowicz P, Tello M, Meyers C, Walsh C, Buttner M, Field R, et al. The 1.6-A resolution crystal structure of NovW: a 4-keto-6-deoxy sugar epimerase from the novobiocin biosynthetic gene cluster of Streptomyces spheroides. Proteins. 2006;63:261-5 pubmed
  24. Pagliai F, Gardner C, Pande S, Lorca G. LVIS553 transcriptional regulator specifically recognizes novobiocin as an effector molecule. J Biol Chem. 2010;285:16921-30 pubmed publisher
    ..Using high throughput screening, novobiocin was found to specifically bind LVIS553 with a K(D) = 33.8 +/- 2...
  25. Sasaki T, Igarashi Y, Saito N, Furumai T. TPU-0031-A and B, new antibiotics of the novobiocin group produced by Streptomyces sp. TP-A0556. J Antibiot (Tokyo). 2001;54:441-7 pubmed
    ..Spectroscopic analyses showed that TPU-0031-A and B were 7'-demethylnovobiocin and 5"-demethylnovobiocin, respectively. These compounds showed antibiotic activity against Gram-positive and -negative bacteria. ..
  26. Eustáquio A, Luft T, Wang Z, Gust B, Chater K, Li S, et al. Novobiocin biosynthesis: inactivation of the putative regulatory gene novE and heterologous expression of genes involved in aminocoumarin ring formation. Arch Microbiol. 2003;180:25-32 pubmed
    The left ends of the biosynthetic gene clusters of novobiocin ( nov), clorobiocin ( clo) and coumermycin A(1) ( cou) from Streptomyces spheroides (syn. S. caeruleus) NCIMB 11891, S. roseochromogenes var. oscitans DS 12.976 and S...
  27. Ivanisevic R, Milic M, Ajdic D, Rakonjac J, Savic D. Nucleotide sequence, mutational analysis, transcriptional start site, and product analysis of nov, the gene which affects Escherichia coli K-12 resistance to the gyrase inhibitor novobiocin. J Bacteriol. 1995;177:1766-71 pubmed
    ..the existence of a gene locus, nov, which affects resistance of Escherichia coli K-12 to the gyrase inhibitor novobiocin and, to a lesser degree, coumermycin (J. Rakonjac, M. Milic, D. Ajdic, D. Santos, R. Ivanisevic, and D. J...
  28. Zhao H, Boyd J, Jolicoeur N, Shen S. A coumermycin/novobiocin-regulated gene expression system. Hum Gene Ther. 2003;14:1619-29 pubmed
    ..More significantly, addition of novobiocin, an antagonist of coumermycin, promptly switched off expression of the gene by abolishing coumermycin-induced ..
  29. Lilic M, Jovanovic M, Jovanovic G, Savic D. Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype. FEMS Microbiol Lett. 2003;224:239-46 pubmed
    ..and Salmonella, along with being auxotrophs for the cysteine, exhibit increased resistance to the antibiotics novobiocin (Nov) and mecillinam...
  30. Shelton S, Shawgo M, Matthews S, Lu Y, Donnelly A, Szabla K, et al. KU135, a novel novobiocin-derived C-terminal inhibitor of the 90-kDa heat shock protein, exerts potent antiproliferative effects in human leukemic cells. Mol Pharmacol. 2009;76:1314-22 pubmed publisher
    ..Here, we investigated the extent to which a novel novobiocin-derived C-terminal Hsp90 inhibitor, designated KU135, induced antiproliferative effects in Jurkat T-lymphocytes...
  31. Chusri S, Villanueva I, Voravuthikunchai S, Davies J. Enhancing antibiotic activity: a strategy to control Acinetobacter infections. J Antimicrob Chemother. 2009;64:1203-11 pubmed publisher
    ..of plant phenolics was tested using growth inhibition assays in combination with subinhibitory concentrations of novobiocin. The antibacterial susceptibilities of susceptible and MDR A...
  32. Hauschild T, Stepanovic S, Zakrzewska Czerwinska J. Staphylococcus stepanovicii sp. nov., a novel novobiocin-resistant oxidase-positive staphylococcal species isolated from wild small mammals. Syst Appl Microbiol. 2010;33:183-7 pubmed publisher
    Five novobiocin-resistant and oxidase-positive staphylococcal strains were isolated from wild small mammals...
  33. Zhao H, Donnelly A, Kusuma B, Brandt G, Brown D, Rajewski R, et al. Engineering an antibiotic to fight cancer: optimization of the novobiocin scaffold to produce anti-proliferative agents. J Med Chem. 2011;54:3839-53 pubmed publisher
    Development of the DNA gyrase inhibitor, novobiocin, into a selective Hsp90 inhibitor was accomplished through structural modifications to the amide side chain, coumarin ring, and sugar moiety...
  34. Williams G, Goff R, Zhang C, Thorson J. Optimizing glycosyltransferase specificity via "hot spot" saturation mutagenesis presents a catalyst for novobiocin glycorandomization. Chem Biol. 2008;15:393-401 pubmed publisher
    ..Incredibly, even in the absence of a high-throughput screen for novobiocic acid glycosylation, this approach rapidly led to improvements in the desired catalytic activity of several hundred-fold. ..
  35. Bellon S, Parsons J, Wei Y, Hayakawa K, Swenson L, Charifson P, et al. Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase. Antimicrob Agents Chemother. 2004;48:1856-64 pubmed
    ..0-A resolution and a 24-kDa N-terminal fragment of the ParE subunit complexed with novobiocin at 2.1-A resolution...
  36. Tropp B, Ragolia L, Xia W, Dowhan W, Milkman R, Rudd K, et al. Identity of the Escherichia coli cls and nov genes. J Bacteriol. 1995;177:5155-7 pubmed
    cls and nov mutants have similar increased sensitivities to novobiocin and reduced levels of cardiolipin, both of which can be corrected by plasmid-borne copies of either wild-type gene...
  37. Oliver S, Gillespie B, Ivey S, Lewis M, Johnson D, Lamar K, et al. Influence of prepartum pirlimycin hydrochloride or penicillin-novobiocin therapy on mastitis in heifers during early lactation. J Dairy Sci. 2004;87:1727-31 pubmed
    ..in 2 dairy research herds to determine whether prepartum therapy of heifer mammary glands with penicillin-novobiocin or pirlimycin hydrochloride was effective for reducing the percentage of heifers and mammary quarters infected ..
  38. Mu oz R, Bustamante M, de la Campa A. Ser-127-to-Leu substitution in the DNA gyrase B subunit of Streptococcus pneumoniae is implicated in novobiocin resistance. J Bacteriol. 1995;177:4166-70 pubmed
    ..The same amino acid substitution (Ser-127 to Leu) confers novobiocin resistance on four isolates of S. pneumoniae...
  39. Wu D, Zhang R, Zhao R, Chen G, Cai Y, Jin J. A novel function of novobiocin: disrupting the interaction of HIF 1? and p300/CBP through direct binding to the HIF1? C-terminal activation domain. PLoS ONE. 2013;8:e62014 pubmed publisher
    ..Here, we document, for the first time, that the aminocoumarin antibiotic, novobiocin, directly blocks the protein-protein interaction between the HIF1? C-terminal activation domain (CTAD) and the ..
  40. Duan P, You G. Novobiocin is a potent inhibitor for human organic anion transporters. Drug Metab Dispos. 2009;37:1203-10 pubmed publisher
    ..Previous studies have shown that novobiocin, an inhibitor for breast cancer resistance proteins (BCRP), inhibited organic anion transport...
  41. Zhao X, Gust B, Heide L. S-Adenosylmethionine (SAM) and antibiotic biosynthesis: effect of external addition of SAM and of overexpression of SAM biosynthesis genes on novobiocin production in Streptomyces. Arch Microbiol. 2010;192:289-97 pubmed publisher
    ..SAM addition, and of the expression of SAM biosynthetic genes, on the production of the aminocoumarin antibiotic novobiocin in the heterologous producer strain Streptomyces coelicolor M512 (nov-BG1)...
  42. Donnelly A, Mays J, Burlison J, Nelson J, Vielhauer G, Holzbeierlein J, et al. The design, synthesis, and evaluation of coumarin ring derivatives of the novobiocin scaffold that exhibit antiproliferative activity. J Org Chem. 2008;73:8901-20 pubmed publisher
    b>Novobiocin, a known DNA gyrase inhibitor, binds to a nucleotide-binding site located on the Hsp90 C-terminus and induces degradation of Hsp90-dependent client proteins at approximately 700 microM in breast cancer cells (SKBr3)...
  43. Dangel V, Eustáquio A, Gust B, Heide L. novE and novG act as positive regulators of novobiocin biosynthesis. Arch Microbiol. 2008;190:509-19 pubmed publisher
    The biosynthetic gene cluster of the aminocoumarin antibiotic novobiocin contains two putative regulatory genes, i.e., novE and novG...
  44. Burlison J, Avila C, Vielhauer G, Lubbers D, Holzbeierlein J, Blagg B. Development of novobiocin analogues that manifest anti-proliferative activity against several cancer cell lines. J Org Chem. 2008;73:2130-7 pubmed publisher
    Recent studies have shown that the DNA gyrase inhibitor, novobiocin, binds to a previously unrecognized ATP-binding site located at the C-terminus of Hsp90 and induces degradation of Hsp90-dependent client proteins at approximately 700 ..
  45. Yun B, Huang W, Leach N, Hartson S, Matts R. Novobiocin induces a distinct conformation of Hsp90 and alters Hsp90-cochaperone-client interactions. Biochemistry. 2004;43:8217-29 pubmed
    ..Recently, novobiocin has been shown to bind to a second nucleotide binding site located within the C-terminal domain of Hsp90...
  46. Gellert M, O Dea M, Itoh T, Tomizawa J. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc Natl Acad Sci U S A. 1976;73:4474-8 pubmed
    b>Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA...
  47. Freel Meyers C, Oberthür M, Anderson J, Kahne D, Walsh C. Initial characterization of novobiocic acid noviosyl transferase activity of NovM in biosynthesis of the antibiotic novobiocin. Biochemistry. 2003;42:4179-89 pubmed
    The aminocoumarin class of antibiotics, exemplified by novobiocin, is composed of tripartite l-noviosylaminocoumarin prenylbenzoate natural products...
  48. Baranova N, Nikaido H. The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate. J Bacteriol. 2002;184:4168-76 pubmed
    Screening of random fragments of Escherichia coli genomic DNA for their ability to increase the novobiocin resistance of a hypersusceptible (Delta)acrAB mutant resulted in the isolation of a plasmid containing baeR, which codes for the ..
  49. Sharp S, Workman P. Inhibitors of the HSP90 molecular chaperone: current status. Adv Cancer Res. 2006;95:323-48 pubmed
    ..Thus, it is not surprising that new HSP90 agents are under development against this novel target for cancer therapy and several show promise. ..
  50. Adachi S, Hiraga S. Mutants suppressing novobiocin hypersensitivity of a mukB null mutation. J Bacteriol. 2003;185:3690-5 pubmed
    ..A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of the mukB null mutation...
  51. Onogi T, Yamazoe M, Ichinose C, Niki H, Hiraga S. Null mutation of the dam or seqA gene suppresses temperature-sensitive lethality but not hypersensitivity to novobiocin of muk null mutants. J Bacteriol. 2000;182:5898-901 pubmed
    ..We show here that the associated muk null mutations cause hypersensitivity to novobiocin. Null mutation of either dam or seqA suppressed partially the temperature-sensitive lethality but failed to ..
  52. Hussy P, Maass G, Tummler B, Grosse F, Schomburg U. Effect of 4-quinolones and novobiocin on calf thymus DNA polymerase alpha primase complex, topoisomerases I and II, and growth of mammalian lymphoblasts. Antimicrob Agents Chemother. 1986;29:1073-8 pubmed
    The influence of ciprofloxacin, nalidixic acid, norfloxacin, novobiocin, and ofloxacin on elements of eucaryotic DNA replication was investigated in vitro...
  53. Vimont A, Delignette Muller M, Vernozy Rozand C. Supplementation of enrichment broths by novobiocin for detecting Shiga toxin-producing Escherichia coli from food: a controversial use. Lett Appl Microbiol. 2007;44:326-31 pubmed
    To investigate the assumption that usage of novobiocin (20 mg l(-1)) in Shiga toxin-producing Escherichia coli (STEC) enrichment broths could achieve false-negative results. First, the minimum inhibitory concentration (MIC) of 74 E...