ouabain

Summary

Summary: A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.

Top Publications

  1. Galuska D, Kotova O, Barres R, Chibalina D, Benziane B, Chibalin A. Altered expression and insulin-induced trafficking of Na+-K+-ATPase in rat skeletal muscle: effects of high-fat diet and exercise. Am J Physiol Endocrinol Metab. 2009;297:E38-49 pubmed publisher
    ..Disturbances in skeletal muscle Na(+)-K(+)-ATPase regulation, particularly the alpha(2)-subunit, may contribute to impaired ion homeostasis in insulin-resistant states such as obesity and type 2 diabetes. ..
  2. Staessen J, Thijs L, Stolarz Skrzypek K, Bacchieri A, Barton J, Espositi E, et al. Main results of the ouabain and adducin for Specific Intervention on Sodium in Hypertension Trial (OASIS-HT): a randomized placebo-controlled phase-2 dose-finding study of rostafuroxin. Trials. 2011;12:13 pubmed publisher
    The Ouabain and Adducin for Specific Intervention on Sodium in Hypertension (OASIS-HT) Trial was a phase-2 dose-finding study of rostafuroxin, a digitoxygenin derivative, which selectively antagonizes the effects of endogenous ouabain (..
  3. Sandtner W, Egwolf B, Khalili Araghi F, Sánchez Rodríguez J, Roux B, Bezanilla F, et al. Ouabain binding site in a functioning Na+/K+ ATPase. J Biol Chem. 2011;286:38177-83 pubmed publisher
    ..Functional studies revealed that ouabain-sensitive residues distributed widely throughout the primary sequence of the protein...
  4. Jornada L, Moretti M, Valvassori S, Ferreira C, Padilha P, Arent C, et al. Effects of mood stabilizers on hippocampus and amygdala BDNF levels in an animal model of mania induced by ouabain. J Psychiatr Res. 2010;44:506-10 pubmed publisher
    ..Intracerebroventricular (ICV) injection of ouabain (OUA), a specific Na(+)/K(+) ATPase inhibitor, induces hyperlocomotion in rats, and has been used as an animal ..
  5. Barwe S, Jordan M, Skay A, Inge L, Rajasekaran S, Wolle D, et al. Dysfunction of ouabain-induced cardiac contractility in mice with heart-specific ablation of Na,K-ATPase beta1-subunit. J Mol Cell Cardiol. 2009;47:552-60 pubmed publisher
    ..that intact hearts of Na,K-beta(1) KO anesthetized mice as well as isolated cardiomyocytes were insensitive to ouabain-induced positive inotropy...
  6. Pulina M, Zulian A, Berra Romani R, Beskina O, Mazzocco Spezzia A, Baryshnikov S, et al. Upregulation of Na+ and Ca2+ transporters in arterial smooth muscle from ouabain-induced hypertensive rats. Am J Physiol Heart Circ Physiol. 2010;298:H263-74 pubmed publisher
    Prolonged ouabain administration (25 microg kg(-1) day(-1) for 5 wk) induces "ouabain hypertension" (OH) in rats, but the molecular mechanisms by which ouabain elevates blood pressure are unknown...
  7. Larre I, Castillo A, Flores Maldonado C, Contreras R, Galván I, Muñoz Estrada J, et al. Ouabain modulates ciliogenesis in epithelial cells. Proc Natl Acad Sci U S A. 2011;108:20591-6 pubmed publisher
    ..In a previous study, we demonstrated that 10 nM ouabain modulates TJs, and we now show that it controls polarity as well...
  8. Ark M, Ozdemir A, Polat B. Ouabain-induced apoptosis and Rho kinase: a novel caspase-2 cleavage site and fragment of Rock-2. Apoptosis. 2010;15:1494-506 pubmed publisher
    b>Ouabain, a specific Na+/K+-ATPase inhibitor, has recently been identified as a mammalian hormone. Its elevated concentrations in human plasma have also been associated with pathogenesis of several diseases...
  9. Sapia L, Palomeque J, Mattiazzi A, Petroff M. Na+/K+-ATPase inhibition by ouabain induces CaMKII-dependent apoptosis in adult rat cardiac myocytes. J Mol Cell Cardiol. 2010;49:459-68 pubmed publisher
    ..Recently, administration of toxic doses of ouabain has been shown to induce cardiac myocyte apoptosis...

More Information

Publications62

  1. Lee Y, Ng K, Lai W, Man C, Lieu D, Lau C, et al. Ouabain facilitates cardiac differentiation of mouse embryonic stem cells through ERK1/2 pathway. Acta Pharmacol Sin. 2011;32:52-61 pubmed publisher
    To investigate the effects of the cardiotonic steroid, ouabain, on cardiac differentiation of murine embyronic stem cells (mESCs)...
  2. Fedorova O, Shapiro J, Bagrov A. Endogenous cardiotonic steroids and salt-sensitive hypertension. Biochim Biophys Acta. 2010;1802:1230-6 pubmed publisher
    ..Recent findings include the specific identification of endogenous cardenolide (endogenous ouabain) and bufadienolide (marinobufagenin) CTS in humans along with the delineation of mechanisms by which CTS can ..
  3. Karpova L, Bulygina E, Boldyrev A. Different neuronal Na(+)/K(+)-ATPase isoforms are involved in diverse signaling pathways. Cell Biochem Funct. 2010;28:135-41 pubmed publisher
    Inhibition of rat neuronal Na(+)/K(+)-ATPase alpha3 isoform at low (100 nM) ouabain concentration led to activation of MAP kinase cascade via PKC and PIP(3) kinase...
  4. Cuozzo F, Raciti M, Bertelli L, Parente R, Di Renzo L. Pro-death and pro-survival properties of ouabain in U937 lymphoma derived cells. J Exp Clin Cancer Res. 2012;31:95 pubmed publisher
    ..In the present investigation we analyzed the pro-death and pro-survival properties of ouabain in the human lymphoma derived cell line U937...
  5. Burlaka I, Liu X, Rebetz J, Arvidsson I, Yang L, Brismar H, et al. Ouabain protects against Shiga toxin-triggered apoptosis by reversing the imbalance between Bax and Bcl-xL. J Am Soc Nephrol. 2013;24:1413-23 pubmed publisher
    ..Signaling via the ouabain/Na,K-ATPase/IP3R/NF-?B pathway increases expression of Bcl-xL, an inhibitor of Bax, suggesting that ouabain might ..
  6. Cao J, He L, Lin G, Hu C, Dong R, Zhang J, et al. Cap-dependent translation initiation factor, eIF4E, is the target for Ouabain-mediated inhibition of HIF-1?. Biochem Pharmacol. 2014;89:20-30 pubmed publisher
    ..In the present study, we observed that neither HIF-1? mRNA levels nor HIF-1? protein degradation are affected by Ouabain. However, Ouabain was found to be associated with the regulation of HIF-1? translation...
  7. Jacobs B, Liu Y, Pulina M, Golovina V, Hamlyn J. Normal pregnancy: mechanisms underlying the paradox of a ouabain-resistant state with elevated endogenous ouabain, suppressed arterial sodium calcium exchange, and low blood pressure. Am J Physiol Heart Circ Physiol. 2012;302:H1317-29 pubmed publisher
    ..Here we test the hypothesis that vascular resistance to endogenous ouabain (EO) develops in normal pregnancy and is mediated by reduced expression of NCX1.3 and TRPCs...
  8. Fu Y, Ding D, Jiang H, Salvi R. Ouabain-induced cochlear degeneration in rat. Neurotox Res. 2012;22:158-69 pubmed publisher
    b>Ouabain, a potent inhibitor of the Na+/K+-ATPase pump, selectively destroys spiral ganglion neurons (SGNs) in gerbils and mice, whereas in guinea pigs it preferentially damages cochlear hair cells...
  9. Zhang Y, Yuan Z, Ge H, Ren Y. Effects of long-term ouabain treatment on blood pressure, sodium excretion, and renal dopamine D(1) receptor levels in rats. J Comp Physiol B. 2010;180:117-24 pubmed publisher
    To examine the effects of chronic ouabain treatment on blood pressure (BP), sodium excretion, and renal dopamine D(1) receptor level, male Sprague-Dawley (SD) rats were treated with ouabain (27...
  10. Queiroz Madeira E, Lara L, Wengert M, Landgraf S, Líbano Soares J, Zapata Sudo G, et al. Na(+)-ATPase in spontaneous hypertensive rats: possible AT(1) receptor target in the development of hypertension. Biochim Biophys Acta. 2010;1798:360-6 pubmed publisher
    ..The present study was designed to examine ouabain-insensitive Na(+)-ATPase activity and its regulation by Ang II in spontaneously hypertensive rats (SHR)...
  11. Silva E, Serrao M, Soares da Silva P. Age-dependent effect of ouabain on renal Na+,K+-ATPase. Life Sci. 2011;88:719-24 pubmed publisher
    This study examines the effect of chronic ouabain-treatment on renal Na(+) handling in 12-week and 52-week old rats. Wistar Kyoto rats aged 5 weeks or 45 weeks were treated with ouabain or vehicle during 7 weeks...
  12. Liu J, Xie Z. The sodium pump and cardiotonic steroids-induced signal transduction protein kinases and calcium-signaling microdomain in regulation of transporter trafficking. Biochim Biophys Acta. 2010;1802:1237-45 pubmed publisher
    ..The plant-derived CTS such as digoxin are valuable drugs for the management of cardiac diseases, whereas ouabain and marinobufagenin (MBG) have been identified as a new class of endogenous hormones...
  13. Leenen F. The central role of the brain aldosterone-"ouabain" pathway in salt-sensitive hypertension. Biochim Biophys Acta. 2010;1802:1132-9 pubmed publisher
    ..These responses appear to depend on activation of a CNS cascade starting with aldosterone-MR-ENaC-"ouabain," the latter lowering neuronal membrane potential leading to enhanced angiotensin II release in e.g. the PVN...
  14. Kim S, Yu H, Park H, Ha K, Kim Y, Shin S, et al. Intracerebroventricular administration of ouabain, a Na/K-ATPase inhibitor, activates mTOR signal pathways and protein translation in the rat frontal cortex. Prog Neuropsychopharmacol Biol Psychiatry. 2013;45:73-82 pubmed publisher
    Intracerebroventricular (ICV) injection of ouabain, a specific Na/K-ATPase inhibitor, induces behavioral changes in rats in a putative animal model of mania...
  15. Wansapura A, Lasko V, Lingrel J, Lorenz J. Mice expressing ouabain-sensitive ?1-Na,K-ATPase have increased susceptibility to pressure overload-induced cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2011;300:H347-55 pubmed publisher
    The Na,K-ATPase is a ubiquitous transmembrane pump and a specific receptor for cardiac glycosides such as ouabain and digoxin, which are used in the management of congestive heart failure (CHF)...
  16. Dvela M, Rosen H, Ben Ami H, Lichtstein D. Endogenous ouabain regulates cell viability. Am J Physiol Cell Physiol. 2012;302:C442-52 pubmed publisher
    The endogenous cardiac steroid-like compounds, endogenous ouabain (EO) in particular, are present in the human circulation and are considered putative ligands of the inhibitory binding site of the plasma membrane Na(+)-K(+)-ATPase...
  17. Pulgar V, Jeffers A, Rashad H, Diz D, Aileru A. Increased constrictor tone induced by ouabain treatment in rats. J Cardiovasc Pharmacol. 2013;62:174-83 pubmed publisher
    b>Ouabain (Oua)-induced hypertension in rodents provides a model to study cardiovascular changes associated with human hypertension. We examined vascular function in rats after a long-term treatment with Oua...
  18. Gasper R, Vandenbussche G, Goormaghtigh E. Ouabain-induced modifications of prostate cancer cell lipidome investigated with mass spectrometry and FTIR spectroscopy. Biochim Biophys Acta. 2011;1808:597-605 pubmed publisher
    ..to investigate modifications of prostate cancer PC-3 cell lipidome after exposure to sub-lethal concentrations of ouabain. FTIR spectroscopy offered an overview of the lipid classes present in the whole sample...
  19. Pousti A, Bakhtiarian A, Najafi R, Deemyad T, Brumand K, Hosseini M. Effect of sertraline on ouabain-induced arrhythmia in isolated guinea-pig atria. Depress Anxiety. 2009;26:E106-10 pubmed publisher
    The effect of sertraline a selective serotonin reuptake inhibitor antidepressant was studied on ouabain-induced toxicity (arrhythmia) in spontaneously beating isolated guinea-pig atria.
  20. Rodrigues Mascarenhas S, Da Silva de Oliveira A, Amoêdo N, Affonso Mitidieri O, Rumjanek F, Rumjanek V. Modulation of the immune system by ouabain. Ann N Y Acad Sci. 2009;1153:153-63 pubmed publisher
    b>Ouabain, a known inhibitor of the Na,K-ATPase, has been shown to regulate a number of lymphocyte functions in vitro and in vivo. Lymphocyte proliferation, apoptosis, cytokine production, and monocyte function are all affected by ouabain...
  21. Ogawa H, Shinoda T, Cornelius F, Toyoshima C. Crystal structure of the sodium-potassium pump (Na+,K+-ATPase) with bound potassium and ouabain. Proc Natl Acad Sci U S A. 2009;106:13742-7 pubmed publisher
    ..Here we describe a crystal structure of Na(+),K(+)-ATPase with bound ouabain, a representative cardiac glycoside, at 2.8 A resolution in a state analogous to E2.2K(+).Pi...
  22. Xu J, Jin R, Li E, Wang Y, Bai Y. Signal pathways in ouabain-induced proliferation of leukemia cells. World J Pediatr. 2009;5:140-5 pubmed publisher
    ..We explored the participation of ouabain-activated signaling pathways in growth regulation of leukemia cells...
  23. Meier S, Tavraz N, Dürr K, Friedrich T. Hyperpolarization-activated inward leakage currents caused by deletion or mutation of carboxy-terminal tyrosines of the Na+/K+-ATPase {alpha} subunit. J Gen Physiol. 2010;135:115-34 pubmed publisher
    ..In the absence of extracellular K(+), the DeltaYY construct mediated ouabain-sensitive, hyperpolarization-activated inward currents, which were Na(+) dependent and increased with ..
  24. Botta P, de Souza F, Sangrey T, De Schutter E, Valenzuela C. Alcohol excites cerebellar Golgi cells by inhibiting the Na+/K+ ATPase. Neuropsychopharmacology. 2010;35:1984-96 pubmed publisher
    ..indicated that ethanol partially inhibits this pump and this effect could be mimicked by low concentrations of ouabain. Partial inhibition of Na(+)/K(+) ATPase function in a computer model of the Golgi cell reproduced these ..
  25. Belliard A, Sottejeau Y, Duan Q, Karabin J, Pierre S. Modulation of cardiac Na+,K+-ATPase cell surface abundance by simulated ischemia-reperfusion and ouabain preconditioning. Am J Physiol Heart Circ Physiol. 2013;304:H94-103 pubmed publisher
    ..survival were investigated in a cellular model of ischemia-reperfusion (I/R)-induced injury and protection by ouabain-induced preconditioning (OPC)...
  26. Cui X, Liu X, Feng H, Zhao S, Gao H. Grape seed proanthocyanidin extracts enhance endothelial nitric oxide synthase expression through 5'-AMP activated protein kinase/Surtuin 1-Krüpple like factor 2 pathway and modulate blood pressure in ouabain induced hypertensive rats. Biol Pharm Bull. 2012;35:2192-7 pubmed
    ..We also illuminated the role of GSPE in hypertension treatment. By chronic administration of GSPE in ouabain induced hypertensive rats model, we access the effect of GSPE on blood pressure regulation and the possible ..
  27. Daniel L, Etkovitz N, Weiss S, Rubinstein S, Ickowicz D, Breitbart H. Regulation of the sperm EGF receptor by ouabain leads to initiation of the acrosome reaction. Dev Biol. 2010;344:650-7 pubmed publisher
    ..b>Ouabain, a known Na/K ATPase blocker is present in the blood and in the female reproductive tract...
  28. Tian J, Li X, Liang M, Liu L, Xie J, Ye Q, et al. Changes in sodium pump expression dictate the effects of ouabain on cell growth. J Biol Chem. 2009;284:14921-9 pubmed publisher
    Here we show that ouabain-induced cell growth regulation is intrinsically coupled to changes in the cellular amount of Na/K-ATPase via the phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway...
  29. Karpova L, Eva A, Kirch U, Boldyrev A, Scheiner Bobis G. Sodium pump alpha1 and alpha3 subunit isoforms mediate distinct responses to ouabain and are both essential for survival of human neuroblastoma. FEBS J. 2010;277:1853-60 pubmed publisher
    ..by means of transfection with small interfering RNA, and investigated cell survival and the cellular response to ouabain. We found that both of the alpha subunits are essential for cell survival, indicating that substitution of one ..
  30. Omotayo T, Rocha J, Ibukun E, Kade I. Inorganic mercury interacts with thiols at the nucleotide and cationic binding sites of the ouabain-sensitive cerebral electrogenic sodium pump. Neurochem Int. 2011;58:776-84 pubmed publisher
    ..Hence, the present study sought to evaluate the interaction of inorganic mercury (Hg(2+)) with the ouabain-sensitive electrogenic pump in partially purified mammalian brain membrane preparations...
  31. Khodus G, Kruusmägi M, Li J, Liu X, Aperia A. Calcium signaling triggered by ouabain protects the embryonic kidney from adverse developmental programming. Pediatr Nephrol. 2011;26:1479-82 pubmed publisher
    ..rat embryonic kidneys, we found that the sodium-potassium-adenosine triphosphatase (Na, K-ATPase) ligand ouabain triggers, via the Na, K-ATPase/ inositol 1,4,5-trisphosphate receptor signalosome, a calcium-nuclear factor-kappa ..
  32. Alfonso A, Fernández Araujo A, Alfonso C, Caramés B, Tobío A, Louzao M, et al. Palytoxin detection and quantification using the fluorescence polarization technique. Anal Biochem. 2012;424:64-70 pubmed publisher
    ..The assay protocol was first developed using ouabain as a binding molecule. The final result was a straight line that correlates FP units and PLT concentration...
  33. Cornelius F, Mahmmoud Y. Interaction between cardiotonic steroids and Na,K-ATPase. Effects of pH and ouabain-induced changes in enzyme conformation. Biochemistry. 2009;48:10056-65 pubmed publisher
    ..The Na,K-ATPase is specifically inhibited by cardiotonic steroids like ouabain, which bind to the extracellular side of the enzyme and is of significant therapeutic value in the treatment of ..
  34. Alonso E, Cano Abad M, Moreno Ortega A, Novalbos J, Milla J, Garcia A, et al. Nanomolar ouabain elicits apoptosis through a direct action on HeLa cell mitochondria. Steroids. 2013;78:1110-8 pubmed publisher
    The steroid Na(+)/K(+) ATPase (NKA) blocker ouabain has been shown to exhibit pro-apoptotic effects in various cell systems; however, the mechanism involved in those effects is unclear...
  35. Laursen M, Yatime L, Nissen P, Fedosova N. Crystal structure of the high-affinity Na+K+-ATPase-ouabain complex with Mg2+ bound in the cation binding site. Proc Natl Acad Sci U S A. 2013;110:10958-63 pubmed publisher
    ..a crystal structure of the phosphorylated pig kidney Na(+),K(+)-ATPase in complex with the CTS representative ouabain, extending to 3.4 Å resolution...
  36. Akimova O, Lopina O, Rubtsov A, Gekle M, Tremblay J, Hamet P, et al. Death of ouabain-treated renal epithelial cells: evidence for p38 MAPK-mediated Na (i) (+) /K (i) (+) -independent signaling. Apoptosis. 2009;14:1266-73 pubmed publisher
    Recent studies demonstrate that cytotoxic actions of ouabain and other cardiotonic steroids (CTS) on renal epithelial cells (REC) are triggered by their interaction with the Na(+),K(+)-ATPase alpha-subunit but not the result of ..
  37. Anderson T, Huguenard J, Prince D. Differential effects of Na+-K+ ATPase blockade on cortical layer V neurons. J Physiol. 2010;588:4401-14 pubmed publisher
    ..Bath perfusion of Na(+)-K(+) ATPase antagonists (ouabain or dihydro-ouabain) induced either a membrane depolarization in current clamp, or inward current under voltage ..
  38. Oshiro N, Dostanic Larson I, Neumann J, Lingrel J. The ouabain-binding site of the ?2 isoform of Na,K-ATPase plays a role in blood pressure regulation during pregnancy. Am J Hypertens. 2010;23:1279-85 pubmed publisher
    The cardiotonic steroid/ouabain-binding site of the ? subunit of Na,K-ATPase is thought to play an important role in cardiovascular homeostasis...
  39. Rocafull M, Romero F, Thomas L, del Castillo J. Isolation and cloning of the K+-independent, ouabain-insensitive Na+-ATPase. Biochim Biophys Acta. 2011;1808:1684-700 pubmed publisher
    ..However, there are functional and biochemical evidences that suggest the existence of a K+-independent, ouabain-insensitive Na+ pump, associated to a Na+-ATPase with similar characteristics, located at basolateral plasma ..
  40. Pelin M, Sosa S, Della Loggia R, Poli M, Tubaro A, Decorti G, et al. The cytotoxic effect of palytoxin on Caco-2 cells hinders their use for in vitro absorption studies. Food Chem Toxicol. 2012;50:206-11 pubmed publisher
    ..These results indicate that, due to the high susceptibility to PLTX cytotoxic effects, Caco-2 cells do not represent an appropriate and reliable model for investigating intestinal barrier permeation by this toxin...
  41. Riegel R, Valvassori S, Elias G, Réus G, Steckert A, de Souza B, et al. Animal model of mania induced by ouabain: Evidence of oxidative stress in submitochondrial particles of the rat brain. Neurochem Int. 2009;55:491-5 pubmed publisher
    The intracerebroventricular (ICV) administration of ouabain (a Na(+)/K(+)-ATPase inhibitor) in rats has been suggested to mimic some symptoms of human bipolar mania...
  42. Hamlyn J, Manunta P. Endogenous ouabain: a link between sodium intake and hypertension. Curr Hypertens Rep. 2011;13:14-20 pubmed publisher
    The sodium pump, an ancestral enzyme with conserved ability to bind ouabain, plays a key role in salt conservation and is regulated by aldosterone and endogenous ouabain (EO)...
  43. Freitas T, Rezin G, Gonçalves C, Jeremias G, Gomes L, Scaini G, et al. Evaluation of citrate synthase activity in brain of rats submitted to an animal model of mania induced by ouabain. Mol Cell Biochem. 2010;341:245-9 pubmed publisher
    ..The intracerebroventricular (i.c.v) administration of ouabain (a Na(+)/K(+)-ATPase inhibitor) in rats has been used as an animal model of mania, because present face, ..
  44. McKenna M, Perry B, Serpiello F, Caldow M, Levinger P, Cameron Smith D, et al. Unchanged [3H]ouabain binding site content but reduced Na+-K+ pump ?2-protein abundance in skeletal muscle in older adults. J Appl Physiol (1985). 2012;113:1505-11 pubmed publisher
    ..3)-isoform gene expression (real-time RT-PCR), protein abundance (immunoblotting), and NKA total content ([(3)H]ouabain binding sites). The Elderly had lower peak oxygen consumption (-36.7%, P = 0.000), strength (-36.3%, P = 0...
  45. Zhang Y, Yuan Z. Dopamine-mediated inhibition of renal Na+/K+-ATPase in HK-2 cells is reduced by ouabain. Clin Exp Pharmacol Physiol. 2010;37:613-8 pubmed publisher
    ..1. Abnormal renal sodium handling is considered a major contributing factor in hypertension associated with chronic ouabain treatment. However, the molecular mechanisms involved in abnormal renal sodium handling have not been elucidated...
  46. Brüning C, Prigol M, Luchese C, Pinton S, Nogueira C. Diphenyl diselenide ameliorates behavioral and oxidative parameters in an animal model of mania induced by ouabain. Prog Neuropsychopharmacol Biol Psychiatry. 2012;38:168-74 pubmed publisher
    Bipolar disorder (BD) is a common and severe mood disorder associated with higher rates of suicide and disability. Ouabain, a Na(+)/K(+)-ATPase inhibitor, induces behavioral changes in rats and has been used as a model of mania...
  47. Jansson K, Nguyen A, Magenheimer B, Reif G, Aramadhaka L, BELLO REUSS E, et al. Endogenous concentrations of ouabain act as a cofactor to stimulate fluid secretion and cyst growth of in vitro ADPKD models via cAMP and EGFR-Src-MEK pathways. Am J Physiol Renal Physiol. 2012;303:F982-90 pubmed publisher
    ..We previously showed that ouabain increases proliferation of cultured human ADPKD cells via stimulation of the EGF receptor (EGFR)-Src-MEK/ERK ..
  48. Larre I, Contreras R, Cereijido M. Ouabain modulates cell contacts as well as functions that depend on cell adhesion. Methods Mol Biol. 2011;763:155-68 pubmed publisher
    b>Ouabain, a toxic of vegetal origin used for centuries to treat heart failure, has recently been demonstrated to have an endogenous counterpart, most probably ouabain itself, which behaves as a hormone...
  49. Wansapura A, Lasko V, Xie Z, Fedorova O, Bagrov A, Lingrel J, et al. Marinobufagenin enhances cardiac contractility in mice with ouabain-sensitive alpha1 Na+-K+-ATPase. Am J Physiol Heart Circ Physiol. 2009;296:H1833-9 pubmed publisher
    ..roles and occur in two different chemical forms in the mammalian circulation: cardenolides, such as ouabain, and bufadienolides, such as marinobufagenin (MBG)...
  50. Chen R, Chung T, Li F, Yang W, Jinn T, Tzen J. Steroid-like compounds in Chinese medicines promote blood circulation via inhibition of Na+/K+ -ATPase. Acta Pharmacol Sin. 2010;31:696-702 pubmed publisher
    ..may act as active components via the same molecular mechanism triggered by cardiac glycosides, such as ouabain. The inhibitory potency of ouabain and the identified steroid-like compounds on Na(+)/K(+)-ATPase activity was ..
  51. Montalbetti N, Fischbarg J. Frequency spectrum of transepithelial potential difference reveals transport-related oscillations. Biophys J. 2009;97:1530-7 pubmed publisher
  52. Sánchez Huerta K, Montes S, Perez Severiano F, Alva Sánchez C, Rios C, Pacheco Rosado J. Hypothyroidism reduces glutamate-synaptic release by ouabain depolarization in rat CA3-hippocampal region. J Neurosci Res. 2012;90:905-12 pubmed publisher
    ..The effects of PDC (a glutamate transporter inhibitor) and ouabain (a Na(+) /K(+) -ATPase inhibitor) infusion on microdialysate glutamate and aspartate levels of CA3 hippocampal ..
  53. Larre I, Lazaro A, Contreras R, Balda M, Matter K, Flores Maldonado C, et al. Ouabain modulates epithelial cell tight junction. Proc Natl Acad Sci U S A. 2010;107:11387-92 pubmed publisher
    Epithelial cells treated with high concentrations of ouabain (e.g., 1 microM) retrieve molecules involved in cell contacts from the plasma membrane and detach from one another and their substrates...