hygromycin b


Summary: Aminoglycoside produced by Streptomyces hygroscopicus. It is used as an anthelmintic against swine infections by large roundworms, nodular worms, and whipworms.

Top Publications

  1. Dutt M, Grosser J. An embryogenic suspension cell culture system for Agrobacterium-mediated transformation of citrus. Plant Cell Rep. 2010;29:1251-60 pubmed publisher
    ..Efficient selection was obtained and escapes were prevented when the antibiotic hygromycin B was used as a selection antibiotic following transformation with an Agrobacterium strain containing hptII in ..
  2. Flowers J, Vaillancourt L. Parameters affecting the efficiency of Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola. Curr Genet. 2005;48:380-8 pubmed
    ..Thus, ATMT may be unsuitable for insertional mutagenesis of C. graminicola without further modification. ..
  3. Makridou P, Burnett C, Landy T, Howard K. Hygromycin B-selected cell lines from GAL4-regulated pUAST constructs. Genesis. 2003;36:83-7 pubmed
    ..This facilitates protein-based studies and abolishes the need to carry out time-consuming and expensive transfections. ..
  4. Olhoft P, Flagel L, Donovan C, Somers D. Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method. Planta. 2003;216:723-35 pubmed
    ..delivery into cotyledonary-node cells with the development of a rapid, efficient selection protocol based on hygromycin B. Wounded cotyledonary-node explants were inoculated with A...
  5. Bakker E. Aminoglycoside and aminocyclitol antibiotics: hygromycin B is an atypical bactericidal compound that exerts effects on cells of Escherichia coli characteristics for bacteriostatic aminocyclitols. J Gen Microbiol. 1992;138:563-9 pubmed
    ..proteins (apparent molecular masses 72 and 60 kDa) that were not made by cells treated with the aminocyclitol hygromycin B, which did not cause misreading...
  6. Talhinhas P, Muthumeenakshi S, Neves Martins J, Oliveira H, Sreenivasaprasad S. Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles. Mol Biotechnol. 2008;39:57-67 pubmed publisher
    ..acutatum leading to the identification of the genes associated with the varied lifestyles. ..
  7. Cseke L, Cseke S, Podila G. High efficiency poplar transformation. Plant Cell Rep. 2007;26:1529-38 pubmed
    ..Likewise, the fact that the protocols are amenable to high-throughput formats makes them better suited for large-scale functional genomics studies in poplars. ..
  8. Maresova L, Sychrova H. Arabidopsis thaliana CHX17 gene complements the kha1 deletion phenotypes in Saccharomyces cerevisiae. Yeast. 2006;23:1167-71 pubmed
    ..e. Nha1p, Nhx1p, Kha1p) could be an ideal tool for expression and functional analysis of any type of similar plant antiporters (plasma membrane, endosomal/prevacuolar and Golgi). ..
  9. Soares R, Velho T, De Moraes L, Azevedo M, Soares C, Felipe M. Hygromycin B-resistance phenotype acquired in Paracoccidioides brasiliensis via plasmid DNA integration. Med Mycol. 2005;43:719-23 pubmed
    Yeast cells of the human pathogenic fungus Paracoccidioides brasiliensis strain Pb01 were transformed to hygromycin B resistance using the plasmid pAN7.1...

More Information


  1. Kemppainen M, Circosta A, Tagu D, Martin F, Pardo A. Agrobacterium-mediated transformation of the ectomycorrhizal symbiont Laccaria bicolor S238N. Mycorrhiza. 2005;16:19-22 pubmed publisher
    ..A. tumefaciens-mediated gene transfer should facilitate future development of insertional mutagenesis, targeted gene disruption and RNA interference technology in L. bicolor. ..
  2. Ali R, Brett C, Mukherjee S, Rao R. Inhibition of sodium/proton exchange by a Rab-GTPase-activating protein regulates endosomal traffic in yeast. J Biol Chem. 2004;279:4498-506 pubmed
    ..Our findings provide a possible molecular link between intraendosomal pH and regulation of vesicular trafficking. ..
  3. Irie T, Honda Y, Hirano T, Sato T, Enei H, Watanabe T, et al. Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals. Appl Microbiol Biotechnol. 2001;56:707-9 pubmed
    It was reported that Pleurotus ostreatus was transformed unstably using recombinant plasmids containing a hygromycin B phosphotransferase gene (hph) under the control of Aspergillus nidulans expression signals, and that the plasmids were ..
  4. Brodersen D, Clemons W, Carter A, Morgan Warren R, Wimberly B, Ramakrishnan V. The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell. 2000;103:1143-54 pubmed
    ..subunit to determine the structures of its complexes with the antibiotics tetracycline, pactamycin, and hygromycin B. The antibiotics bind to discrete sites on the 30S subunit in a manner consistent with much but not all ..
  5. Salauze D, Otal I, Gomez Lus R, Davies J. Aminoglycoside acetyltransferase 3-IV (aacC4) and hygromycin B 4-I phosphotransferase (hphB) in bacteria isolated from human and animal sources. Antimicrob Agents Chemother. 1990;34:1915-20 pubmed
    ..an enzyme of the aminoglycoside acetyltransferase 3 class (AAC-3-IV) (apramycin and gentamicin resistance) and hygromycin B phosphotransferase 4 (HPH-4-I) (hygromycin B resistance) have been isolated from human clinical sources in ..
  6. De Backer M, de Hoogt R, Froyen G, Odds F, Simons F, Contreras R, et al. Single allele knock-out of Candida albicans CGT1 leads to unexpected resistance to hygromycin B and elevated temperature. Microbiology. 2000;146 ( Pt 2):353-65 pubmed
    ..The disrupted strain was significantly more resistant to hygromycin B, an antibiotic which decreases translational fidelity, and showed increased resistance to heat stress...
  7. Ro Y, Scheffter S, Patterson J. Hygromycin B resistance mediates elimination of Leishmania virus from persistently infected parasites. J Virol. 1997;71:8991-8 pubmed
    ..guyanensis. After 6 weeks of drug selection (hygromycin B), transfected parasites lacked detectable quantities of viral genomic double-stranded RNA, viral capsid protein,..
  8. Banuelos M, Moreno D, Olson D, Nguyen Q, Ricarte F, Aguilera Sandoval C, et al. Genomic analysis of severe hypersensitivity to hygromycin B reveals linkage to vacuolar defects and new vacuolar gene functions in Saccharomyces cerevisiae. Curr Genet. 2010;56:121-37 pubmed publisher
    ..Several yeast mutants defective in such vacuolar events have been unable to grow at low levels of hygromycin B, an aminoglycoside antibiotic...
  9. Borovinskaya M, Shoji S, Fredrick K, Cate J. Structural basis for hygromycin B inhibition of protein biosynthesis. RNA. 2008;14:1590-9 pubmed publisher
    ..b>Hygromycin B is an atypical aminoglycoside antibiotic with unique structural and functional properties...
  10. Punt P, Oliver R, Dingemanse M, Pouwels P, van den Hondel C. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene. 1987;56:117-24 pubmed
    ..This marker is based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph). Expression of the hph gene is controlled by A...
  11. Mukherjee S, Kallay L, Brett C, Rao R. Mutational analysis of the intramembranous H10 loop of yeast Nhx1 reveals a critical role in ion homoeostasis and vesicle trafficking. Biochem J. 2006;398:97-105 pubmed
    ..The present study demonstrates the importance of the H10 loop of the NHE family, highlights the differences between plasma membrane and intracellular isoforms and shows that trafficking defects are tightly coupled with pH homoeostasis. ..
  12. Mach R, Schindler M, Kubicek C. Transformation of Trichoderma reesei based on hygromycin B resistance using homologous expression signals. Curr Genet. 1994;25:567-70 pubmed
    Trichoderma reesei was transformed to hygromycin B resistance using a novel vector, which contains the E...
  13. Basso L, Bartiss A, Mao Y, Gast C, Coelho P, Snyder M, et al. Transformation of Candida albicans with a synthetic hygromycin B resistance gene. Yeast. 2010;27:1039-48 pubmed publisher
    ..We found that multiple C. albicans strains were inhibited by hygromycin B, so we designed a 1026 bp gene (CaHygB) that encodes Escherichia coli hygromycin B phosphotransferase with C...
  14. Gaggelli E, Gaggelli N, Molteni E, Valensin G, Balenci D, Wrońska M, et al. Coordination pattern, solution structure and DNA damage studies of the copper(II) complex with the unusual aminoglycoside antibiotic hygromycin B. Dalton Trans. 2010;39:9830-7 pubmed publisher
    The aminoglycosidic antibiotic hygromycin B presents a peculiar chemical structure, characterized by two sugar rings joined via a spiro connection...
  15. Stogios P, Shakya T, Evdokimova E, Savchenko A, Wright G. Structure and function of APH(4)-Ia, a hygromycin B resistance enzyme. J Biol Chem. 2011;286:1966-75 pubmed publisher
    ..APH(4)-Ia is one of two enzymes responsible for bacterial resistance to the atypical aminoglycoside antibiotic hygromycin B (hygB). The crystal structure of APH(4)-Ia enzyme was solved in complex with hygB at 1.95 Å resolution...
  16. Scholtmeijer K, Wösten H, Springer J, Wessels J. Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune. Appl Environ Microbiol. 2001;67:481-3 pubmed
    ..Here we show that intron-dependent mRNA accumulation and truncation of transcripts are two independent events that both affect expression of the bacterial hygromycin B resistance gene in S. commune.
  17. Ganoza M, Kiel M. A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob Agents Chemother. 2001;45:2813-9 pubmed
    ..Here, we show that the antibiotic hygromycin B selectively inhibits the ATPase activity of RbbA...
  18. Pfister P, Risch M, Brodersen D, Bottger E. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003;47:1496-502 pubmed
    b>Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic ribosomes...
  19. Berthold P, Schmitt R, Mages W. An engineered Streptomyces hygroscopicus aph 7" gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii. Protist. 2002;153:401-12 pubmed
    ..Transformants selected on plates with 10 microg/ml hygromycin B exhibited diverse levels of resistance of up to 200 microg/ml that were stably maintained for at least seven ..
  20. Radman I, Greiss S, Chin J. Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection. PLoS ONE. 2013;8:e76019 pubmed publisher
    ..The method combines biolistic bombardment with selection for transgenics that bear a hygromycin B resistance gene on agar plates supplemented with hygromycin B, taking advantage of our observation that ..
  21. McGaha S, Champney W. Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli. Antimicrob Agents Chemother. 2007;51:591-6 pubmed
    The aminoglycoside antibiotic hygromycin B was examined in Escherichia coli cells for inhibitory effects on translation and ribosomal-subunit formation...
  22. Hanif M, Pardo A, Gorfer M, Raudaskoski M. T-DNA transfer and integration in the ectomycorrhizal fungus Suillus bovinus using hygromycin B as a selectable marker. Curr Genet. 2002;41:183-8 pubmed
    ..this system, dikaryotic mycelium of the ectomycorrhizal fungus Suillus bovinus was transformed with recombinant hygromycin B phosphotransferase (hph)and enhanced green fluorescent protein (EGFP) genes fused with a heterologous fungal ..
  23. Dibrov S, Parsons J, Hermann T. A model for the study of ligand binding to the ribosomal RNA helix h44. Nucleic Acids Res. 2010;38:4458-65 pubmed publisher
    ..that HX RNA carrying a fluorescent 2-aminopurine modification provides a model system that can be used to monitor ligand binding to both the ribosomal decoding site and, through an indirect effect, the hygromycin B interaction region.
  24. Kim Y, Kuk Y, Kim K. Inheritance and expression of transgenes through anther culture of transgenic hot pepper. Z Naturforsch C. 2007;62:743-6 pubmed
    ..They grew normally and showed similar phenotypes to the wild-type. Therefore, the use of an anther culture system coupled with genetic transformation in breeding programs will greatly facilitate the genetic improvement of pepper plants. ..
  25. Joshi P, Webb J, Davies J, McMaster W. The gene encoding streptothricin acetyltransferase (sat) as a selectable marker for Leishmania expression vectors. Gene. 1995;156:145-9 pubmed
    ..Selection was based on resistance to the aminoglycosides, hygromycin B and neomycin, and to nourseothricin, a novel independent selectable marker for transfection of Leishmania...
  26. Ulbert S, Chaves I, Borst P. Expression site activation in Trypanosoma brucei with three marked variant surface glycoprotein gene expression sites. Mol Biochem Parasitol. 2002;120:225-35 pubmed
    ..Whereas, a "silent" ES shows a low level of expression of promoter proximal sequences, the level of activation can be reversibly increased, leading to partially activated ESs. ..
  27. Schebelle L, Wolf C, Stribl C, Javaheri T, Schnütgen F, Ettinger A, et al. Efficient conditional and promoter-specific in vivo expression of cDNAs of choice by taking advantage of recombinase-mediated cassette exchange using FlEx gene traps. Nucleic Acids Res. 2010;38:e106 pubmed publisher
    ..The use of the RMCE system presented in this work requires one single-cloning step followed by one overnight gateway clonase reaction and subsequent cassette exchange in ES cells with efficiencies of 40% in average. ..
  28. Gong M, Meng L, Liu W, Zhang J, Shou C. [Establishment of a stable and regulable 293 cell line expressing mycoplasma hyorhinis protein P37]. Beijing Da Xue Xue Bao Yi Xue Ban. 2005;37:575-8 pubmed
    ..To establish a stable cell line, which can express P37 protein of mycoplasma hyorhinis and be regulated by tetracycline, for investigating the effect of p37 on phenotype of cells and its mechanism...
  29. Field S, Johnson R, Mortensen R, Papaioannou V, Spiegelman B, Greenberg M. Growth and differentiation of embryonic stem cells that lack an intact c-fos gene. Proc Natl Acad Sci U S A. 1992;89:9306-10 pubmed
    ..We conclude that despite a large body of literature suggesting an important role for c-fos in cell growth and differentiation, in at least some cell types this gene is not essential for these processes. ..
  30. Nakazawa M, Matsui M. Selection of hygromycin-resistant Arabidopsis seedlings. Biotechniques. 2003;34:28-30 pubmed
  31. Li H, Pan C, Chen H, Zhao C, Lu G, Wang Z. [Optimization of T-dNA insertional mutagenesis and analysis of mutants of Magnaporthe grisea]. Sheng Wu Gong Cheng Xue Bao. 2003;19:419-23 pubmed
    ..S. Kang's laboratory at the Pennsylvania State University, which carries the bacterial hygromycin B phosphotransferase gene (hph) under the control of the Aspergillus nidulans trpC promoter as a selectable ..
  32. Atkins S, Mauchline T, Kerry B, Hirsch P. Development of a transformation system for the nematophagous fungus Pochonia chlamydosporia. Mycol Res. 2004;108:654-61 pubmed
    ..It was unclear whether the introduced plasmids were able to replicate autonomously in P. chlamydosporia, or if they integrated transiently into the fungal genome. Possible reasons for the instability of the transformants are discussed. ..
  33. Deng X, Wei Z, An H. Transgenic peanut plants obtained by particle bombardment via somatic embryogenesis regeneration system. Cell Res. 2001;11:156-60 pubmed
    ..The production of transgenic plants are mainly based on a newly improved somatic embryogenesis regeneration system developed by us. ..
  34. Muñoz Centeno M, Martín Guevara C, Flores A, Pérez Pulido A, Antúnez Rodríguez C, Castillo A, et al. Mpg2 interacts and cooperates with Mpg1 to maintain yeast glycosylation. FEMS Yeast Res. 2012;12:511-20 pubmed publisher
    ..In the absence of Mpg2, defects in cell growth and sensitivity to hygromycin B are observed...
  35. Wu L, El Mezawy A, Shah S. A seed coat outer integument-specific promoter for Brassica napus. Plant Cell Rep. 2011;30:75-80 pubmed publisher
    ..Therefore, the AtGILT promoter can be used as a canola seed coat outer integument-specific promoter after the generation and selection of desired transformants from several transgenic lines. ..
  36. Kubota A, Ishizaki K, Hosaka M, Kohchi T. Efficient Agrobacterium-mediated transformation of the liverwort Marchantia polymorpha using regenerating thalli. Biosci Biotechnol Biochem. 2013;77:167-72 pubmed
    ..This Agrobacterium-mediated transformation system for M. polymorpha should provide opportunities to perform genetic transformation without preparing spores and to generate a sufficient number of transformants with isogenic background...
  37. Kular R, Yehiely F, Kotlo K, Cilensek Z, Bedi R, Deiss L. GAGE, an antiapoptotic protein binds and modulates the expression of nucleophosmin/B23 and interferon regulatory factor 1. J Interferon Cytokine Res. 2009;29:645-55 pubmed publisher
    ..As GAGE is silent in most healthy tissues, targeting GAGE could result in therapeutic interventions in cancer therapy. ..
  38. Kamiya H, Uchiyama M, Nakatsu Y, Tsuzuki T, Harashima H. Effects of target sequence and sense versus anti-sense strands on gene correction with single-stranded DNA fragments. J Biochem. 2008;144:431-6 pubmed publisher
    ..These results suggest that the sense ssDNA fragments are useful for the correction of mutated genes. The variation in the correction efficiency may depend on the sequence of the target position in double-stranded DNA. ..
  39. Draper J, Mur L, Jenkins G, Ghosh Biswas G, Bablak P, Hasterok R, et al. Brachypodium distachyon. A new model system for functional genomics in grasses. Plant Physiol. 2001;127:1539-55 pubmed
    ..Despite its small stature, B. distachyon has large seeds that should prove useful for studies on grain filling. Such biological characteristics represent important traits for study in temperate cereals. ..
  40. Cox G, Toffaletti D, Perfect J. Dominant selection system for use in Cryptococcus neoformans. J Med Vet Mycol. 1996;34:385-91 pubmed
    ..selection system for transformation of wild-type strains of cryptococci in which resistance to the antibiotic hygromycin B is used as the selectable marker...
  41. Hogan L, Klein B. Transforming DNA integrates at multiple sites in the dimorphic fungal pathogen Blastomyces dermatitidis. Gene. 1997;186:219-26 pubmed
    ..of the multinucleate yeasts was achieved by electroporation of DNAs containing a dominant selectable marker, hygromycin B (HygB) resistance...
  42. Providenti M, Mautner S, Chaudhry O, Bombardier M, Scroggins R, Gregorich E, et al. Determining the environmental fate of a filamentous fungus, Trichoderma reesei, in laboratory-contained intact soil-core microcosms using competitive PCR and viability plating. Can J Microbiol. 2004;50:623-31 pubmed
    ..Throughout the experiment, QM6A#4 remained viable. These results indicate that, following release into the environment, live T. reesei will persist in soil for at least 2 seasons. ..
  43. Dev K, Maheshwari R. Silencing of hygromycin phosphotransferase (hph) gene during sexual cycle and its reversible inactivation in heterokaryon of Neurospora crassa. Curr Microbiol. 2003;47:220-5 pubmed
    ..A heterokaryon between silenced and non-silenced strains was able to grow on hygromycin-containing medium, showing that silencing was recessive. Silencing was reversed in homokaryotic nuclei extracted from such heterokaryon. ..
  44. Dalton S, Bettany A, Bhat V, Gupta M, Bailey K, Timms E, et al. Genetic transformation of Dichanthium annulatum (Forssk)--an apomictic tropical forage grass. Plant Cell Rep. 2003;21:974-80 pubmed
    ..All three of the transformants set seed. Hygromycin resistance varied from 68-100% in the progeny of the three transformants. Transgene transmission appeared to have been mainly through apomixis. ..
  45. Masante C, El Najjar F, Chang A, Jones A, Moncman C, Dutch R. The human metapneumovirus small hydrophobic protein has properties consistent with those of a viroporin and can modulate viral fusogenic activity. J Virol. 2014;88:6423-33 pubmed publisher
    ..In addition, HMPV SH expression resulted in increases in permeability to hygromycin B and alteration of subcellular localization of a fluorescent dye, indicating that SH affects membrane ..
  46. Wang C, Guan X, Wang H, Li G, Dong X, Wang G, et al. Agrobacterium tumefaciens-mediated transformation of Valsa mali: an efficient tool for random insertion mutagenesis. ScientificWorldJournal. 2013;2013:968432 pubmed publisher
    ..were randomly selected for further confirmation and the results showed the presence of T-DNA in all hygromycin B resistant transformants and also revealed random and single gene integration with genetic stability...
  47. Min K, Oh S, Yoon K, Kim C, Lee S. Prolonged gene expression in primary porcine pancreatic cells using an Epstein-Barr virus-based episomal vector. Biochem Biophys Res Commun. 2003;305:108-15 pubmed
    ..were preferentially transfected and conveniently enriched among the mixed primary cell populations using a hygromycin B selection...
  48. Shawlot W, Deng J, Fohn L, Behringer R. Restricted beta-galactosidase expression of a hygromycin-lacZ gene targeted to the beta-actin locus and embryonic lethality of beta-actin mutant mice. Transgenic Res. 1998;7:95-103 pubmed
    ..5 (E8.5) but became growth retarded at E9.5 and subsequently died. The RT-PCR data indicate that this targeted mutation is a hypomorphic allele of beta-actin. ..
  49. Okamoto T, Yamada M, Sekiya S, Okuhara T, Taguchi G, Inatomi S, et al. Agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of the cultivated mushroom Flammulina velutipes. Biosci Biotechnol Biochem. 2010;74:2327-9 pubmed
    ..tumefaciens was used to transform the vegetative dikaryotic mycelium of Flammulina velutipes using a hygromycin B resistance gene as selectable marker...
  50. Atta H, Abul Hamd A, Radwan H. Production of destomycin-A antibiotic by Streptomyces sp. using rice straw as fermented substrate. Commun Agric Appl Biol Sci. 2009;74:879-97 pubmed
    ..The purified antimicrobial agent was suggestive of being belonging to Destomycin-A antibiotic produced by Streptomyces rimosus, AZ-H-A5. ..
  51. Rong J, Song Z, Su J, Xia H, Lu B, Wang F. Low frequency of transgene flow from Bt/CpTI rice to its non-transgenic counterparts planted at close spacing. New Phytol. 2005;168:559-66 pubmed
    ..0%), even if the GM rice is planted at close spacing with non-GM rice, and high densities of GM rice cultivated in the neighborhood of non-GM rice will increase the probability of outcrossing with the non-GM rice. ..
  52. Nyilasi I, Acs K, Papp T, Nagy E, Vagvolgyi C. Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides. Folia Microbiol (Praha). 2005;50:415-20 pubmed
    ..A method was also developed for the hygromycin B-based selection of Mucor transformants...
  53. Theile D, Staffen B, Weiss J. ATP-binding cassette transporters as pitfalls in selection of transgenic cells. Anal Biochem. 2010;399:246-50 pubmed publisher
    ..Consciousness of the results is supposed to guide the molecular biologist to the right choice of adequate experimental conditions for successful selection of genetically engineered eukaryotic cells. ..