limbus corneae

Summary

Summary: An annular transitional zone, approximately 1 mm wide, between the cornea and the bulbar conjunctiva and sclera. It is highly vascular and is involved in the metabolism of the cornea. It is ophthalmologically significant in that it appears on the outer surface of the eyeball as a slight furrow, marking the line between the clear cornea and the sclera. (Dictionary of Visual Science, 3d ed)

Top Publications

  1. Mi S, Chen B, Wright B, Connon C. Ex vivo construction of an artificial ocular surface by combination of corneal limbal epithelial cells and a compressed collagen scaffold containing keratocytes. Tissue Eng Part A. 2010;16:2091-100 pubmed publisher
  2. Mi S, Chen B, Wright B, Connon C. Plastic compression of a collagen gel forms a much improved scaffold for ocular surface tissue engineering over conventional collagen gels. J Biomed Mater Res A. 2010;95:447-53 pubmed publisher
    ..This study demonstrated that the compressed collagen gel was an excellent biomaterial scaffold highly suited to the construction of an artificial corneal epithelium and a significant improvement upon conventional collagen gels...
  3. García I, Etxebarria J, Boto de los Bueis A, Diaz Valle D, Rivas L, Martínez Soroa I, et al. Comparative study of limbal stem cell deficiency diagnosis methods: detection of MUC5AC mRNA and goblet cells in corneal epithelium. Ophthalmology. 2012;119:923-9 pubmed publisher
  4. Kagemann L, Wollstein G, Ishikawa H, Nadler Z, Sigal I, Folio L, et al. Visualization of the conventional outflow pathway in the living human eye. Ophthalmology. 2012;119:1563-8 pubmed publisher
    ..We sought to visualize the aqueous outflow system in 3 dimensions (3D) in living human eyes, and to investigate the use of commercially available spectral-domain optical coherence tomographic (SD-OCT) systems for this purpose...
  5. Baylis O, Figueiredo F, Henein C, Lako M, Ahmad S. 13 years of cultured limbal epithelial cell therapy: a review of the outcomes. J Cell Biochem. 2011;112:993-1002 pubmed publisher
    ..Here, we review the outcomes of this procedure over the past 13 years with the aim of highlighting the best culture and surgical techniques to date...
  6. Bhatia R, Srivastava R, Ghosh A. Limbal stem cell study in contact lens wearers. Ann Ophthalmol (Skokie). 2009;41:87-92 pubmed
    ..Different results were seen as compared to controls in all the four quadrants, but more severe in cases known to cause limbal stem cell deficiency...
  7. Li G, Zhu Y, Xie H, Chen S, Tseng S. Mesenchymal stem cells derived from human limbal niche cells. Invest Ophthalmol Vis Sci. 2012;53:5686-97 pubmed publisher
    ..We investigated whether human limbal niche cells generate mesenchymal stem cells...
  8. Aslan D, Akata R, Holme H, Vulliamy T, Dokal I. Limbal stem cell deficiency in patients with inherited stem cell disorder of dyskeratosis congenita. Int Ophthalmol. 2012;32:615-22 pubmed publisher
  9. Barbaro V, Ferrari S, Fasolo A, Pedrotti E, Marchini G, Sbabo A, et al. Evaluation of ocular surface disorders: a new diagnostic tool based on impression cytology and confocal laser scanning microscopy. Br J Ophthalmol. 2010;94:926-32 pubmed publisher
    ..To provide a new tool for the evaluation of altered ocular surfaces by using a combination of impression cytology, laser scanning confocal microscopy and advanced image analysis...

More Information

Publications62

  1. Shahdadfar A, Haug K, Pathak M, Drolsum L, Olstad O, Johnsen E, et al. Ex vivo expanded autologous limbal epithelial cells on amniotic membrane using a culture medium with human serum as single supplement. Exp Eye Res. 2012;97:1-9 pubmed publisher
  2. Notara M, Schrader S, Daniels J. The porcine limbal epithelial stem cell niche as a new model for the study of transplanted tissue-engineered human limbal epithelial cells. Tissue Eng Part A. 2011;17:741-50 pubmed publisher
    ..These data have significant clinical value as this model can provide information on LESC fate post-transplantation and their ability to respond to injury, which is not possible to study in patients...
  3. Menzel Severing J, Kruse F, Schlötzer Schrehardt U. Stem cell-based therapy for corneal epithelial reconstruction: present and future. Can J Ophthalmol. 2013;48:13-21 pubmed publisher
  4. Ghoubay Benallaoua D, Basli E, Goldschmidt P, Pecha F, Chaumeil C, Laroche L, et al. Human epithelial cell cultures from superficial limbal explants. Mol Vis. 2011;17:341-54 pubmed
    ..To study the kinetics of growth and the phenotype of cells cultured from human limbal explants in a cholera toxin-free medium with no feeder cell layer...
  5. Li J, Riau A, Setiawan M, Mehta J, Ti S, Tong L, et al. S100A expression in normal corneal-limbal epithelial cells and ocular surface squamous cell carcinoma tissue. Mol Vis. 2011;17:2263-71 pubmed
    ..To study the expression and cellular distribution of multiple S100A genes and proteins in normal corneal-limbal epithelium and ocular surface squamous cell carcinoma (SCC) tissue...
  6. Kulkarni B, Tighe P, Mohammed I, Yeung A, Powe D, Hopkinson A, et al. Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics. BMC Genomics. 2010;11:526 pubmed publisher
    ..The purpose of this microarray study was to characterise the transcriptional profile of the LEC and compare with other ocular surface epithelial regions to support our hypothesis that LEC preferentially harbours stem cells (SC)...
  7. Sejpal K, Yu F, Aldave A. The Boston keratoprosthesis in the management of corneal limbal stem cell deficiency. Cornea. 2011;30:1187-94 pubmed publisher
    ..To report the outcomes of the Boston type I keratoprosthesis (Massachusetts Eye and Ear Infirmary, Boston, MA) in the management of corneal limbal stem cell deficiency (LSCD)...
  8. Schornack M. Limbal stem cell disease: management with scleral lenses. Clin Exp Optom. 2011;94:592-4 pubmed publisher
    ..Limbal stem cell deficiency (LSCD) results from damage to or destruction of corneal stem cells...
  9. Notara M, Shortt A, Galatowicz G, Calder V, Daniels J. IL6 and the human limbal stem cell niche: a mediator of epithelial-stromal interaction. Stem Cell Res. 2010;5:188-200 pubmed publisher
    ..STAT3 and IL6 inhibition in 3.1SF cultures significantly reduced HLE colony-forming efficiency, suggesting a previously undetected STAT3-mediated involvement of IL6 in the maintenance of HLE cells in a progenitor-like state...
  10. Miri A, Alomar T, Nubile M, Al Aqaba M, Lanzini M, Fares U, et al. In vivo confocal microscopic findings in patients with limbal stem cell deficiency. Br J Ophthalmol. 2012;96:523-9 pubmed publisher
    ..To describe in vivo confocal microscopy (IVCM) findings in patients with limbal stem cell deficiency (LSCD)...
  11. Chen B, Mi S, Wright B, Connon C. Differentiation status of limbal epithelial cells cultured on intact and denuded amniotic membrane before and after air-lifting. Tissue Eng Part A. 2010;16:2721-9 pubmed publisher
  12. O Callaghan A, Daniels J. Concise review: limbal epithelial stem cell therapy: controversies and challenges. Stem Cells. 2011;29:1923-32 pubmed publisher
    ..In this review, some of the challenges and controversies associated with cultured LESC therapy will be discussed including alternative stem cell sources...
  13. Li G, Chen S, Xie H, Zhu Y, Tseng S. Angiogenesis potential of human limbal stromal niche cells. Invest Ophthalmol Vis Sci. 2012;53:3357-67 pubmed publisher
    ..The perivascular localization of stem cell (SC) niches suggests the presence of a vascular niche. We aimed to determine the angiogenesis potential of limbal niche cells (NCs)...
  14. Merjava S, Neuwirth A, Tanzerova M, Jirsova K. The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva. Histol Histopathol. 2011;26:323-31 pubmed publisher
    ..The detailed characterization of the corneal, limbal and perilimbal conjunctival epithelium under normal circumstances may be useful for characterizing the changes occurring under pathological conditions...
  15. Sun T, Tseng S, Lavker R. Location of corneal epithelial stem cells. Nature. 2010;463:E10-1; discussion E11 pubmed publisher
    ..We point out here that these suggestions are inconsistent with many known growth, differentiation and cell migration properties of the anterior ocular epithelia...
  16. Gomes J, Geraldes Monteiro B, Melo G, Smith R, Cavenaghi Pereira da Silva M, Lizier N, et al. Corneal reconstruction with tissue-engineered cell sheets composed of human immature dental pulp stem cells. Invest Ophthalmol Vis Sci. 2010;51:1408-14 pubmed publisher
  17. Nieto Miguel T, Calonge M, de la Mata A, López Paniagua M, Galindo S, de la Paz M, et al. A comparison of stem cell-related gene expression in the progenitor-rich limbal epithelium and the differentiating central corneal epithelium. Mol Vis. 2011;17:2102-17 pubmed
    ..The objective of this study was to find new putative markers for these SCs and to identify associated molecular pathways...
  18. Zhou J, Chen F, Xiao J, Li C, Liu Y, Ding Y, et al. Enhanced functional properties of corneal epithelial cells by coculture with embryonic stem cells via the integrin ?1-FAK-PI3K/Akt pathway. Int J Biochem Cell Biol. 2011;43:1168-77 pubmed publisher
    ..These findings demonstrated that the functional properties of stem-like corneal epithelial cells were enhanced by cocultured embryonic stem cells via activation of the integrin ?1-FAK-PI3K/Akt signalling pathway...
  19. Deng S, Sejpal K, Tang Q, Aldave A, Lee O, Yu F. Characterization of limbal stem cell deficiency by in vivo laser scanning confocal microscopy: a microstructural approach. Arch Ophthalmol. 2012;130:440-5 pubmed publisher
    ..To evaluate the cellular changes in the corneal epithelium and surrounding structures in limbal stem cell deficiency (LSCD) by using in vivo laser scanning confocal microscopy...
  20. Ordonez P, Di Girolamo N. Limbal epithelial stem cells: role of the niche microenvironment. Stem Cells. 2012;30:100-7 pubmed publisher
  21. Sangwan V, Basu S, Vemuganti G, Sejpal K, Subramaniam S, Bandyopadhyay S, et al. Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study. Br J Ophthalmol. 2011;95:1525-9 pubmed publisher
    ..Ocular burns can damage the corneal epithelial stem cells located at the limbus. This study evaluated the efficacy of xeno-free autologous cell-based treatment of limbal stem cell deficiency...
  22. Hayashi Y, Watanabe N, Ohashi Y. The "replacement hypothesis": corneal stem cell origin epithelia are replaced by limbal stem cell origin epithelia in mouse cornea during maturation. Cornea. 2012;31 Suppl 1:S68-73 pubmed publisher
    ..This study tested the replacement hypothesis, which suggests that corneal stem cell origin epithelia may be replaced by limbal stem cell origin epithelia after 2 weeks of age in mice...
  23. Sangwan V, Jain V, Vemuganti G, Murthy S. Vernal keratoconjunctivitis with limbal stem cell deficiency. Cornea. 2011;30:491-6 pubmed
    ..To describe the clinical features of patients affected with vernal keratoconjunctivitis (VKC) with associated limbal stem cell deficiency (LSCD)...
  24. Ang L, Tanioka H, Kawasaki S, Ang L, Yamasaki K, Do T, et al. Cultivated human conjunctival epithelial transplantation for total limbal stem cell deficiency. Invest Ophthalmol Vis Sci. 2010;51:758-64 pubmed publisher
    ..To determine the feasibility of cultivated conjunctiva as a viable epithelial sheet for transplantation and corneal resurfacing in eyes with limbal stem cell deficiency (LSCD)...
  25. Varghese V, Prasad T, Kumary T. Optimization of culture conditions for an efficient xeno-feeder free limbal cell culture system towards ocular surface regeneration. Microsc Res Tech. 2010;73:1045-52 pubmed publisher
    ..This study will be a major step in the development of xeno-feeder free epithelial equivalents towards ocular surface reconstruction...
  26. Dua H, Miri A, Said D. Contemporary limbal stem cell transplantation - a review. Clin Exp Ophthalmol. 2010;38:104-17 pubmed publisher
    ..Despite its complexities limbal transplantation does significantly improve vision related quality of life. Autografts give the best results and living related donor grafts are next best. Majority of cadaver grafts fail in 5 years...
  27. Albert R, Veréb Z, Csomós K, Moe M, Johnsen E, Olstad O, et al. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium. PLoS ONE. 2012;7:e47187 pubmed publisher
  28. Echevarria T, Di Girolamo N. Tissue-regenerating, vision-restoring corneal epithelial stem cells. Stem Cell Rev. 2011;7:256-68 pubmed publisher
    ..Determining where donor stem cells reside on the recipient's ocular surface and how long they remain viable will provide further insights into improving current therapeutic options for patients with LSCD...
  29. Meller D, Thomasen H, Steuhl K. [Ocular surface reconstruction in limbal stem cell deficiency : Transplantation of cultivated limbal epithelium]. Ophthalmologe. 2012;109:863-8 pubmed publisher
    ..In this article the challenges and controversies associated with these stem cell culture techniques for ocular surface reconstruction are reviewed...
  30. Di Iorio E, Kaye S, Ponzin D, Barbaro V, Ferrari S, Böhm E, et al. Limbal stem cell deficiency and ocular phenotype in ectrodactyly-ectodermal dysplasia-clefting syndrome caused by p63 mutations. Ophthalmology. 2012;119:74-83 pubmed publisher
    ..To describe the ocular phenotype in patients with ectrodactyly-ectodermal dysplasia-clefting (EEC) syndrome (MIM#604292) and to determine the pathogenic basis of visual morbidity...
  31. Rama P, Matuska S, Paganoni G, Spinelli A, De Luca M, Pellegrini G. Limbal stem-cell therapy and long-term corneal regeneration. N Engl J Med. 2010;363:147-55 pubmed publisher
    ..We investigated the long-term clinical results of cell therapy in patients with burn-related corneal destruction associated with limbal stem-cell deficiency, a highly disabling ocular disease...
  32. Ainscough S, Linn M, Barnard Z, Schwab I, Harkin D. Effects of fibroblast origin and phenotype on the proliferative potential of limbal epithelial progenitor cells. Exp Eye Res. 2011;92:10-9 pubmed publisher
    ..Superior growth of LEPs in the presence of limbal fibroblasts indicates a role for limbal fibroblasts in promoting the proliferation of limbal epithelium during wound healing...
  33. Zarei Ghanavati S, Ramirez Miranda A, Deng S. Limbal lacuna: a novel limbal structure detected by in vivo laser scanning confocal microscopy. Ophthalmic Surg Lasers Imaging. 2011;42 Online:e129-31 pubmed publisher
    ..This well-protected location could better shield limbal stem/progenitor cells from damage and might serve as an alternative niche for limbal stem/progenitor cells...
  34. Castro Muñozledo F, Gómez Flores E. Challenges to the study of asymmetric cell division in corneal and limbal epithelia. Exp Eye Res. 2011;92:4-9 pubmed publisher
    ..Future studies should be oriented to understand the regulation of the asymmetrical/symmetrical division, and the control of cell fate by the niche...
  35. Sangwan V, Basu S, MacNeil S, Balasubramanian D. Simple limbal epithelial transplantation (SLET): a novel surgical technique for the treatment of unilateral limbal stem cell deficiency. Br J Ophthalmol. 2012;96:931-4 pubmed publisher
    ..Although long-term results are awaited, this simple limbal epithelial transplantation promises to be an easy and effective technique for treating unilateral limbal stem cell deficiency following ocular burns...
  36. Miri A, Al Deiri B, Dua H. Long-term outcomes of autolimbal and allolimbal transplants. Ophthalmology. 2010;117:1207-13 pubmed publisher
    ..To evaluate the long-term results of limbal stem cell transplantation (LSCT) in patients with ocular surface (OS) disease...
  37. Meyer Blazejewska E, Call M, Yamanaka O, Liu H, Schlötzer Schrehardt U, Kruse F, et al. From hair to cornea: toward the therapeutic use of hair follicle-derived stem cells in the treatment of limbal stem cell deficiency. Stem Cells. 2011;29:57-66 pubmed publisher
    ..These data highlight the therapeutic properties of using HFSC to treat LSCD in a mouse model while demonstrating a strong translational potential and points to the niche as a key factor for determining stem cell differentiation...
  38. Douvaras P, Webb S, Whitaker D, Dorà N, Hill R, Dorin J, et al. Rare corneal clones in mice suggest an age-related decrease of stem cell activity and support the limbal epithelial stem cell hypothesis. Stem Cell Res. 2012;8:109-19 pubmed publisher
    ..Pax6(+/-), KRT5(LacZ/-) corneas had small patches rather than stripes, which confirms that corneal maintenance is abnormal in Pax6(+/-) mice...
  39. Nakatsu M, Ding Z, Ng M, Truong T, Yu F, Deng S. Wnt/?-catenin signaling regulates proliferation of human cornea epithelial stem/progenitor cells. Invest Ophthalmol Vis Sci. 2011;52:4734-41 pubmed publisher
    ..To investigate the expression and role of the Wnt signaling pathway in human limbal stem cells (LSCs)...
  40. Sharma S, Tandon R, Mohanty S, Sharma N, M V, Sen S, et al. Culture of corneal limbal epithelial stem cells: experience from benchtop to bedside in a tertiary care hospital in India. Cornea. 2011;30:1223-32 pubmed publisher
    ..To standardize the technique of ex vivo culture of limbal epithelial stem cells (LESCs) using locally available adapted methods and evaluate the outcome of transplantation in patients with limbal stem cell deficiency...
  41. Tan E, He H, Tseng S. Epidermal differentiation and loss of clonal growth potential of human limbal basal epithelial progenitor cells during intrastromal invasion. Invest Ophthalmol Vis Sci. 2011;52:4534-45 pubmed publisher
    ..It remains unclear whether intrastromal invasion is contingent on culturing conditions and whether invaded cells retain their progenitor status and original lineage...
  42. Ordonez P, Chow S, Wakefield D, Di Girolamo N. Human limbal epithelial progenitor cells express ?v?5-integrin and the interferon-inducible chemokine CXCL10/IP-10. Stem Cell Res. 2013;11:888-901 pubmed publisher
    ..Knowledge of the niche's molecular composition and the genes expressed by its SC will facilitate isolation and maintenance of these cells for therapeutic purposes. ..
  43. Meyer Blazejewska E, Kruse F, Bitterer K, Meyer C, Hofmann Rummelt C, Wünsch P, et al. Preservation of the limbal stem cell phenotype by appropriate culture techniques. Invest Ophthalmol Vis Sci. 2010;51:765-74 pubmed publisher
  44. Liu J, Lawrence B, Liu A, Schwab I, Oliveira L, Rosenblatt M. Silk fibroin as a biomaterial substrate for corneal epithelial cell sheet generation. Invest Ophthalmol Vis Sci. 2012;53:4130-8 pubmed publisher
    ..To evaluate a silk fibroin (SF) biomaterial as a substrate for corneal epithelial cell proliferation, differentiation, and stratification in vitro compared with denuded human amniotic membrane (AM)...
  45. Zajicova A, Pokorna K, Lencova A, Krulova M, Svobodova E, Kubinova S, et al. Treatment of ocular surface injuries by limbal and mesenchymal stem cells growing on nanofiber scaffolds. Cell Transplant. 2010;19:1281-90 pubmed publisher
    ..The results thus show that nanofibers prepared from copolymer PA6/12 represent a convenient scaffold for growth of LSCs and MSCs and transfer to treat SC deficiencies and various ocular surface injuries...
  46. Xie H, Chen S, Li G, Tseng S. Limbal epithelial stem/progenitor cells attract stromal niche cells by SDF-1/CXCR4 signaling to prevent differentiation. Stem Cells. 2011;29:1874-85 pubmed publisher
    ..This novel in vitro model of sphere growth with NCs can be used for investigating how limbal SC self-renewal and fate decision might be regulated in the limbal niche...
  47. Tseng S, Chen S, Shen Y, Chen W, Hu F. Critical appraisal of ex vivo expansion of human limbal epithelial stem cells. Curr Mol Med. 2010;10:841-50 pubmed
    ..Such an approach may one day help realize considerable promise held by adult SCs in treating a number of diseases...
  48. Biber J, Skeens H, Neff K, Holland E. The cincinnati procedure: technique and outcomes of combined living-related conjunctival limbal allografts and keratolimbal allografts in severe ocular surface failure. Cornea. 2011;30:765-71 pubmed publisher
  49. Skeens H, Brooks B, Holland E. Congenital aniridia variant: minimally abnormal irides with severe limbal stem cell deficiency. Ophthalmology. 2011;118:1260-4 pubmed publisher
    ..To clinically and molecularly characterize a group of patients with progressive limbal stem cell deficiency (LSCD) due to aniridic keratopathy (AK), but with minimally affected irides...
  50. Paris F, Gonçalves E, Barros J, Campos M, Sato E, Gomes J. Impression cytology findings in bullous keratopathy. Br J Ophthalmol. 2010;94:773-6 pubmed publisher
    ..To evaluate, by impression cytology (IC), the cytological features of the central area of corneal epithelial surface of patients with symptomatic bullous keratopathy (BK). Design Cross-sectional observational case series...
  51. Notara M, Alatza A, Gilfillan J, Harris A, Levis H, Schrader S, et al. In sickness and in health: Corneal epithelial stem cell biology, pathology and therapy. Exp Eye Res. 2010;90:188-95 pubmed publisher
    ..The clinical precedence for use of stem cell therapy and the accessibility of the transparent stem cell niche make the cornea a unique model for the study of adult stem cells in physiological conditions as well as in disease...
  52. Higa K, Kato N, Yoshida S, Ogawa Y, Shimazaki J, Tsubota K, et al. Aquaporin 1-positive stromal niche-like cells directly interact with N-cadherin-positive clusters in the basal limbal epithelium. Stem Cell Res. 2013;10:147-55 pubmed publisher
  53. Ma D, Chen H, Lai J, Sun C, Wang S, Lin K, et al. Matrix revolution: molecular mechanism for inflammatory corneal neovascularization and restoration of corneal avascularity by epithelial stem cell transplantation. Ocul Surf. 2009;7:128-44 pubmed
    ..Taken together, the merits of enhanced anti-angiogenic activity and the preservation of corneal epithelial stem cells encourage further application of this tissue engineering technique for ocular surface reconstruction. ..