corneal epithelium

Summary

Summary: Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.

Top Publications

  1. Mantelli F, ARGUESO P. Functions of ocular surface mucins in health and disease. Curr Opin Allergy Clin Immunol. 2008;8:477-83 pubmed publisher
    ..The purpose of the present review is to describe new concepts on the role of mucins in the protection of corneal and conjunctival epithelia and to identify alterations of mucins in ocular surface diseases...
  2. Liu Y, Ding Y, Ma P, Wu Z, Duan H, Liu Z, et al. Enhancement of long-term proliferative capacity of rabbit corneal epithelial cells by embryonic stem cell conditioned medium. Tissue Eng Part C Methods. 2010;16:793-802 pubmed publisher
    ..Our findings indicate that this new ESC-CM culture system can generate low-immunogenic autologous cells sufficiently for use in regenerative medicine...
  3. Reinach P, Pokorny K. The corneal epithelium: clinical relevance of cytokine-mediated responses to maintenance of corneal health. Arq Bras Oftalmol. 2008;71:80-6 pubmed
  4. Yanai R, Ko J, Morishige N, Chikama T, Ichijima H, Nishida T. Disruption of zonula occludens-1 localization in the rabbit corneal epithelium by contact lens-induced hypoxia. Invest Ophthalmol Vis Sci. 2009;50:4605-10 pubmed publisher
    Hypoxia impairs the barrier function of the corneal epithelium. This function depends on tight junctions, of which zonula occludens (ZO)-1 is a major component...
  5. Takacs L, Toth E, Berta A, Vereb G. Stem cells of the adult cornea: from cytometric markers to therapeutic applications. Cytometry A. 2009;75:54-66 pubmed publisher
    ..In the adult organism, the outermost corneal epithelium is the most exposed to environmental damage, and its constant renewal is assured by the epithelial stem ..
  6. Ma P, Bian F, Wang Z, Zheng X, Chotikavanich S, Pflugfelder S, et al. Human corneal epithelium-derived thymic stromal lymphopoietin links the innate and adaptive immune responses via TLRs and Th2 cytokines. Invest Ophthalmol Vis Sci. 2009;50:2702-9 pubmed publisher
    To explore the crucial role of the human corneal epithelium-derived proallergic cytokine thymic stromal lymphopoietin (TSLP) in initiation and regulation of immune responses.
  7. Gu S, Xing C, Han J, Tso M, Hong J. Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo. Mol Vis. 2009;15:99-107 pubmed
    ..To examine whether bone marrow mesenchymal stem cells (MSCs) could be differentiated into corneal epithelial cells in vivo and ex vivo...
  8. Huang C, Cheng H, Lin K, Cheng J, Tsai J, Liao W, et al. Tamoxifen-induced [Ca2+]i rise and apoptosis in corneal epithelial cells. Toxicology. 2009;255:58-64 pubmed publisher
    ..Tamoxifen-caused cytotoxicity was partly mediated by a Ca2+-independent apoptotic pathway...
  9. Cao L, Zhang X, Liu X, Chen T, Zhao M. Chloride channels and transporters in human corneal epithelium. Exp Eye Res. 2010;90:771-9 pubmed publisher
    ..immunostaining confirmed protein expression and distribution of CLC-2, CLC-3, CLC-4, CLC-6 and CFTR in human corneal epithelium. CLC-2 preferentially labeled the apical and basal layers, while CLC-3 and CLC-4 labeled only the ..

More Information

Publications62

  1. Nagai N, Murao T, Okamoto N, Ito Y. Comparison of corneal wound healing rates after instillation of commercially available latanoprost and travoprost in rat debrided corneal epithelium. J Oleo Sci. 2010;59:135-41 pubmed
    ..and zinc chloride (ZnCl(2)) and latanoprost eye drops with benzalkonium chloride (BAC), using rat debrided corneal epithelium. Rat corneal epithelium was removed with a BD Micro-Sharp, and the wounded corneas were dyed with a 1% ..
  2. Vellonen K, Hakli M, Merezhinskaya N, Tervo T, Honkakoski P, Urtti A. Monocarboxylate transport in human corneal epithelium and cell lines. Eur J Pharm Sci. 2010;39:241-7 pubmed publisher
    ..of the present study was to assess the expression and transporter role of human MCT1, MCT3 and MCT4 in the corneal epithelium, corneal epithelial cell lines (primary HCEpiC and immortalized HCE cells) and isolated rabbit corneas...
  3. Qi H, Shine H, Li D, de Paiva C, Farley W, Jones D, et al. Glial cell-derived neurotrophic factor gene delivery enhances survival of human corneal epithelium in culture and the overexpression of GDNF in bioengineered constructs. Exp Eye Res. 2008;87:580-6 pubmed publisher
    ..Our findings revealed that GDNF is a survival growth factor for cultured human corneal epithelium. The use of bioengineered human corneal constructs containing GDNF-transduced epithelial cells represents a ..
  4. Hao J, Li S, Liu C, Kao W. Electrically assisted delivery of macromolecules into the corneal epithelium. Exp Eye Res. 2009;89:934-41 pubmed publisher
    ..of electrically assisted delivery of macromolecules such as small interfering RNA (siRNA) into the corneal epithelium, to optimize the iontophoresis and electroporation methods, and to study the mechanisms of corneal ..
  5. Pauloin T, Dutot M, Joly F, Warnet J, Rat P. High molecular weight hyaluronan decreases UVB-induced apoptosis and inflammation in human epithelial corneal cells. Mol Vis. 2009;15:577-83 pubmed
    ..The aim of this study was to investigate high molecular weight hyaluronan (HMW-HA) protection on human corneal epithelial (HCE) cells against ultraviolet B (UVB) radiation-induced toxic effects...
  6. Khoh Reiter S, Jessen B. Evaluation of the cytotoxic effects of ophthalmic solutions containing benzalkonium chloride on corneal epithelium using an organotypic 3-D model. BMC Ophthalmol. 2009;9:5 pubmed publisher
  7. Mort R, Ramaesh T, Kleinjan D, Morley S, West J. Mosaic analysis of stem cell function and wound healing in the mouse corneal epithelium. BMC Dev Biol. 2009;9:4 pubmed publisher
    The mouse corneal epithelium is a continuously renewing 5-6 cell thick protective layer covering the corneal surface, which regenerates rapidly when injured...
  8. Zhang J, Cavet M, Ward K. Anti-inflammatory effects of besifloxacin, a novel fluoroquinolone, in primary human corneal epithelial cells. Curr Eye Res. 2008;33:923-32 pubmed publisher
    ..The aim of this study was to determine the anti-inflammatory effects of besifloxacin, a novel fluoroquinolone under clinical evaluation for the treatment of ophthalmic infections, in human corneal epithelial cells (HCEpiC)...
  9. Usui T, Sugisaki K, Iriyama A, Yokoo S, Yamagami S, Nagai N, et al. Inhibition of corneal neovascularization by blocking the angiotensin II type 1 receptor. Invest Ophthalmol Vis Sci. 2008;49:4370-6 pubmed publisher
    ..To determine the role of angiotensin II type 1 receptor (AT1R) signaling in corneal neovascularization...
  10. Dua H, Miri A, Alomar T, Yeung A, Said D. The role of limbal stem cells in corneal epithelial maintenance: testing the dogma. Ophthalmology. 2009;116:856-63 pubmed publisher
  11. Li Y, Piatigorsky J. Targeted deletion of Dicer disrupts lens morphogenesis, corneal epithelium stratification, and whole eye development. Dev Dyn. 2009;238:2388-400 pubmed publisher
    ..roles of Dicer and miRNAs in eye development by conditionally deleting the Dicer gene in the mouse lens and corneal epithelium. Adult Dicer conditional null (DicerCN) mice had severe microphthalmia with no discernible lens and a poorly ..
  12. Nagai N, Murao T, Ito Y, Okamoto N, Sasaki M. Enhancing effects of sericin on corneal wound healing in rat debrided corneal epithelium. Biol Pharm Bull. 2009;32:933-6 pubmed
    ..We demonstrate the effects of sericin on corneal wound healing in rat debrided corneal epithelium. We also determined the effects of sericin on cell adhesion and proliferation in a human cornea epithelial ..
  13. Barbosa F, Góes R, de Faria E Sousa S, Haddad A. Regeneration of the corneal epithelium after debridement of its central region: an autoradiographic study on rabbits. Curr Eye Res. 2009;34:636-45 pubmed
    ..To investigate the proliferative behavior of the corneal and limbal epithelia after debridement on the central region of the rabbit cornea...
  14. Zhang J, Cavet M, VanDerMeid K, Salvador Silva M, Lopez F, Ward K. BOL-303242-X, a novel selective glucocorticoid receptor agonist, with full anti-inflammatory properties in human ocular cells. Mol Vis. 2009;15:2606-16 pubmed
    ..Data from in vitro and in vivo studies suggest an improved side-effect profile of this compound compared to classical glucocorticoids. The aim of this study was to determine the anti-inflammatory effect of BOL-303242-X in ocular cells...
  15. Chen M, Hu D, Pan Z, Lu C, Xue C, Aass I. Curcumin protects against hyperosmoticity-induced IL-1beta elevation in human corneal epithelial cell via MAPK pathways. Exp Eye Res. 2010;90:437-43 pubmed publisher
    ..The present study suggests that curcumin might have therapeutic potential for treating dry eye disease...
  16. Morishige N, Ko J, Morita Y, Nishida T. Expression of semaphorin 3A in the rat corneal epithelium during wound healing. Biochem Biophys Res Commun. 2010;395:451-7 pubmed publisher
    ..We have now further investigated the localization of Sema3A in the normal rat corneal epithelium as well as changes in its expression pattern during wound healing after central corneal epithelial ..
  17. Ma X, Shimmura S, Miyashita H, Yoshida S, Kubota M, Kawakita T, et al. Long-term culture and growth kinetics of murine corneal epithelial cells expanded from single corneas. Invest Ophthalmol Vis Sci. 2009;50:2716-21 pubmed publisher
    ..To develop a reproducible procedure for the long-term culture of corneal epithelial cells from a single mouse cornea...
  18. Hara Y, Shiraishi A, Kobayashi T, Kadota Y, Shirakata Y, Hashimoto K, et al. Alteration of TLR3 pathways by glucocorticoids may be responsible for immunosusceptibility of human corneal epithelial cells to viral infections. Mol Vis. 2009;15:937-48 pubmed
    ..In addition, we determined the effects of immunosuppressive drugs on the innate responses...
  19. Liu H, Begley C, Chen M, Bradley A, Bonanno J, McNamara N, et al. A link between tear instability and hyperosmolarity in dry eye. Invest Ophthalmol Vis Sci. 2009;50:3671-9 pubmed publisher
    ..The purpose of this study was to provide indirect evidence of short-term hyperosmolar conditions during tear instability and to test whether the corneal epithelium responds to transient hyperosmolar stress.
  20. Wang Z, Yang H, Zhang F, Pan Z, Capó Aponte J, Reinach P. Dependence of EGF-induced increases in corneal epithelial proliferation and migration on GSK-3 inactivation. Invest Ophthalmol Vis Sci. 2009;50:4828-35 pubmed publisher
  21. Jiang T, Cai L, Ji W, Hui Y, Wang Y, Hu D, et al. Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats. Mol Vis. 2010;16:1304-16 pubmed
    ..To explore the feasibility of bone marrow mesenchymal stem cells (MSCs) transdifferentiating into corneal epithelial cells in a limbal stem cell deficiency (LSCD) model in rats...
  22. Seo M, Kim J, Lee M, Sohn Y, Kang K, Yoo M. The therapeutic effect of DA-6034 on ocular inflammation via suppression of MMP-9 and inflammatory cytokines and activation of the MAPK signaling pathway in an experimental dry eye model. Curr Eye Res. 2010;35:165-75 pubmed publisher
    ..In addition, to elucidate the mechanism of DA-6034, we evaluated the mitogen-activated protein kinase (MAPK) signaling pathway and transcriptional factor-kappa B (NF-kB) in corneal epithelial cells...
  23. Sun T, Tseng S, Lavker R. Location of corneal epithelial stem cells. Nature. 2010;463:E10-1; discussion E11 pubmed publisher
    ..are expressed, however, in corneal basal cells, thus distinguishing the mode of keratin expression in corneal epithelium from that of all other stratified epithelia), the centripetal migration of corneal epithelial cells, the ..
  24. Langford M, Redmond P, Chanis R, Misra R, Redens T. Glutamate, excitatory amino acid transporters, Xc- antiporter, glutamine synthetase, and gamma-glutamyltranspeptidase in human corneal epithelium. Curr Eye Res. 2010;35:202-11 pubmed publisher
    The viability and functions of the corneal epithelium are dependent in large measure on the active uptake of nutrients, growth factors, and amino acids from stroma and tear...
  25. Pauly A, Meloni M, Brignole Baudouin F, Warnet J, Baudouin C. Multiple endpoint analysis of the 3D-reconstituted corneal epithelium after treatment with benzalkonium chloride: early detection of toxic damage. Invest Ophthalmol Vis Sci. 2009;50:1644-52 pubmed publisher
    ..To investigate the effects of benzalkonium chloride (BAK) on the human reconstituted corneal epithelial model (HCE) and to optimize the operating potential of this model in the field of ophthalmic toxicology...
  26. Scorolli L, Meduri A, Morara M, Scalinci S, Greco P, Meduri R, et al. Effect of cysteine in transgenic mice on healing of corneal epithelium after excimer laser photoablation. Ophthalmologica. 2008;222:380-5 pubmed publisher
    ..To evaluate the role of prepared cysteine per os in corneal epithelial healing in transgenic mice (B6(A)-Rpe65rd12/J) after excimer laser photoablation...
  27. Zhao J, Mo V, Nagasaki T. Distribution of label-retaining cells in the limbal epithelium of a mouse eye. J Histochem Cytochem. 2009;57:177-85 pubmed publisher
    ..These results show that LRCs are present asymmetrically in a narrow zone within the limbus that can be defined precisely in reference to a newly defined anatomical boundary line between the cornea and the limbus...
  28. Katoli P, Sharif N, Sule A, Dimitrijevich S. NPR-B natriuretic peptide receptors in human corneal epithelium: mRNA, immunohistochemistochemical, protein, and biochemical pharmacology studies. Mol Vis. 2010;16:1241-52 pubmed
    ..peptide receptors (NPRs) in primary human corneal epithelial cells (p-CEPI), SV40-immortalized CEPI cells (CEPI-17-CL4) and in human corneal epithelium, and to define the pharmacology of natriuretic peptide (NP)-induced cGMP accumulation.
  29. Wiechmann A, Hollaway L, Rada J. Melatonin receptor expression in Xenopus laevis surface corneal epithelium: diurnal rhythm of lateral membrane localization. Mol Vis. 2009;15:2384-403 pubmed
    ..are seven-pass G protein-coupled receptors located in many tissues throughout the body, including the corneal epithelium (CE), and relay circadian signals to the target cells...
  30. Lim M, Hurst R, Konynenbelt B, Ubels J. Cytotoxicity testing of multipurpose contact lens solutions using monolayer and stratified cultures of human corneal epithelial cells. Eye Contact Lens. 2009;35:287-96 pubmed publisher
    ..To investigate in vitro cytotoxicity of five marketed multipurpose contact lens solutions (MPS) on the morphology, viability, and barrier function of monolayer and stratified human corneal-limbal epithelial cells...
  31. Vellonen K, Mannermaa E, Turner H, Hakli M, Wolosin J, Tervo T, et al. Effluxing ABC transporters in human corneal epithelium. J Pharm Sci. 2010;99:1087-98 pubmed publisher
    ..in the cell models, but they showed over-expression of most efflux transporters compared to that of normal corneal epithelium. In conclusion, MRP1, MRP5, and BCRP are expressed in the corneal epithelium, but MDR1, MRP2, MRP3, MRP4, ..
  32. Yang K, Jiang Z, Wang D, Lian X, Yang T. Corneal epithelial-like transdifferentiation of hair follicle stem cells is mediated by pax6 and beta-catenin/Lef-1. Cell Biol Int. 2009;33:861-6 pubmed publisher
    ..Bulge KSCs may have potential therapeutic application as cell source for the construction of bioengineered corneas...
  33. Yamasaki K, Kawasaki S, Young R, Fukuoka H, Tanioka H, Nakatsukasa M, et al. Genomic aberrations and cellular heterogeneity in SV40-immortalized human corneal epithelial cells. Invest Ophthalmol Vis Sci. 2009;50:604-13 pubmed publisher
    ..The nature of this cell line was assessed for genomic aberrations and cellular heterogeneity...
  34. Majo F, Rochat A, Nicolas M, Jaoudé G, Barrandon Y. Oligopotent stem cells are distributed throughout the mammalian ocular surface. Nature. 2008;456:250-4 pubmed publisher
    ..The corneal epithelium is a squamous epithelium that is constantly renewing, with a vertical turnover of 7 to 14 days in many ..
  35. Nyunt A, Ishida Y, Yu Y, Shimada S. Topical apolipoprotein A-1 may have a beneficial effect on the corneal epithelium in a mouse model of dry eye: a pilot study. Eye Contact Lens. 2008;34:287-92 pubmed publisher
    ..The goal of this study was to evaluate the effect of apolipoprotein A (ApoA)-1 and its synergistic action with d-pantethine (DP) on corneal epithelial disorders in dry-eye mouse model...
  36. Qi H, Li D, Shine H, Chen Z, Yoon K, Jones D, et al. Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells. Exp Eye Res. 2008;86:34-40 pubmed
    ..The spatial expression of NGF and TrkA by small clusters of basal cells interspersed between negative cell patches suggests that they are potential markers for human corneal epithelial progenitor cells...
  37. Chang C, Green C, McGhee C, Sherwin T. Acute wound healing in the human central corneal epithelium appears to be independent of limbal stem cell influence. Invest Ophthalmol Vis Sci. 2008;49:5279-86 pubmed publisher
    ..These migrate centripetally, differentiate outward to the surface, and are then lost by desquamation. This study was conducted to analyze the contribution of human central corneal epithelial cells toward corneal epithelial regeneration...
  38. Grueb M, Bartz Schmidt K, Rohrbach J. Adrenergic regulation of cAMP/protein kinase A pathway in corneal epithelium and endothelium. Ophthalmic Res. 2008;40:322-8 pubmed publisher
    ..The aim of this study was to characterize subtypes of adrenergic G-protein-coupled receptors and their influence on cAMP concentration and PKA activity in bovine corneal epithelial and endothelial cells...
  39. Chen Z, Tong L, Li Z, Yoon K, Qi H, Farley W, et al. Hyperosmolarity-induced cornification of human corneal epithelial cells is regulated by JNK MAPK. Invest Ophthalmol Vis Sci. 2008;49:539-49 pubmed publisher
  40. Chang S, Chou S, Chuang J. Mechanical corneal epithelium scraping and ethanol treatment up-regulate cytokine gene expression differently in rabbit cornea. J Refract Surg. 2008;24:150-9 pubmed
    ..To explore inflammation and wound healing in the rabbit eye following topical ethanol treatment or mechanical debridement...
  41. de la Fuente M, Seijo B, Alonso M. Bioadhesive hyaluronan-chitosan nanoparticles can transport genes across the ocular mucosa and transfect ocular tissue. Gene Ther. 2008;15:668-76 pubmed publisher
    ..in mind, we first evaluated the transfection efficiency of the plasmid DNA-loaded nanoparticles in a human corneal epithelium cell model...
  42. Lyngholm M, Vorum H, Nielsen K, Østergaard M, Honoré B, Ehlers N. Differences in the protein expression in limbal versus central human corneal epithelium--a search for stem cell markers. Exp Eye Res. 2008;87:96-105 pubmed publisher
    ..purpose of this study was to characterize differences in protein expression between human central and limbal corneal epithelium by a proteomic approach using two-dimensional polyacrylamide gel electrophoresis (2D PAGE) combined with ..
  43. Qi H, Li D, Bian F, Chuang E, Jones D, Pflugfelder S. Expression of glial cell-derived neurotrophic factor and its receptor in the stem-cell-containing human limbal epithelium. Br J Ophthalmol. 2008;92:1269-74 pubmed publisher
  44. Kim S, Ha B, Kim E, Tchah H, Kim T. The effect of topical bevacizumab on corneal neovascularization. Ophthalmology. 2008;115:e33-8 pubmed publisher
    ..To examine the effect of topical bevacizumab on corneal neovascularization (NV) over a period of 3 months...
  45. Ehlers N, Hjortdal J, Nielsen K, Thiel H, Ørntoft T. Phenotypic variability in Meesmann's dystrophy: clinical review of the literature and presentation of a family genetically identical to the original family. Acta Ophthalmol. 2008;86:40-4 pubmed
    To describe the phenotypic variability in Meesmann's microcystic dystrophy of the corneal epithelium based on a review of the literature and the presentation of a Danish family.
  46. Lyngholm M, Høyer P, Vorum H, Nielsen K, Ehlers N, Møllgård K. Immunohistochemical markers for corneal stem cells in the early developing human eye. Exp Eye Res. 2008;87:115-21 pubmed publisher
    The corneal epithelium is continuously being renewed. Differentiated epithelial cells originate from limbal stem cells (LSCs) located in the periphery of the cornea, the corneoscleral limbus...
  47. Barrandon Y. Crossing boundaries: stem cells, holoclones, and the fundamentals of squamous epithelial renewal. Cornea. 2007;26:S10-2 pubmed
    ..Understanding why and how the cornea has evolved differently from all other stratified epithelia is certainly a major challenge in epithelial stem cell biology...
  48. Okada Y, Ikeda K, Yamanaka O, Miyamoto T, Kitano A, Kao W, et al. TNFalpha suppression of corneal epithelium migration. Mol Vis. 2007;13:1428-35 pubmed
    ..To evaluate the role of tumor necrosis factor alpha (TNFalpha) in regulation of corneal epithelial cell migration...
  49. Yokoo S, Yamagami S, Shimada T, Usui T, Sato T, Amano S, et al. A novel isolation technique of progenitor cells in human corneal epithelium using non-tissue culture dishes. Stem Cells. 2008;26:1743-8 pubmed publisher
    The existence of adult stem cells or progenitor cells in the human corneal epithelium (i.e., self-renewing squamous cells) has long been suggested, but these cells have not yet been isolated...
  50. Chikama T, Liu C, Meij J, Hayashi Y, Wang I, Yang L, et al. Excess FGF-7 in corneal epithelium causes corneal intraepithelial neoplasia in young mice and epithelium hyperplasia in adult mice. Am J Pathol. 2008;172:638-49 pubmed publisher
    ..the effects of excess fibroblast growth factor-7 (FGF-7) on both the proliferation and differentiation of corneal epithelium in a novel Krt12-rtTA/tet-O-FGF-7 double transgenic mouse model in which cornea-specific FGF-7 ..
  51. Poulaki V, Colby K. Genetics of anterior and stromal corneal dystrophies. Semin Ophthalmol. 2008;23:9-17 pubmed publisher
    ..It is highly likely that even when our understanding of the molecular basis of the corneal dystrophies is complete, this knowledge will be used as an adjunct to clinical findings to make the diagnosis of a corneal dystrophy...
  52. Talarico E, Mangini N. Alternative splice variants of plasma membrane calcium-ATPases in human corneal epithelium. Exp Eye Res. 2007;85:869-79 pubmed
    ..Our previous work shows that all four PMCAs are expressed and have specific locations in human corneal epithelium (hCE). The present work examined which splice variants of PMCAs are expressed in hCE...
  53. Chikama T, Wakuta M, Liu Y, Nishida T. Deviated mechanism of wound healing in diabetic corneas. Cornea. 2007;26:S75-81 pubmed
    We examined phenotypic changes during the wound healing process in the corneal epithelium of Goto-Kakizaki (GK) rats, a spontaneous model of type 2 diabetes mellitus...