centromere protein b


Summary: A DNA-binding protein that interacts with a 17-base pair sequence known as the CENP-B box motif. The protein is localized constitutively to the CENTROMERE and plays an important role in its maintenance.

Top Publications

  1. Yoda K, Kitagawa K, Masumoto H, Muro Y, Okazaki T. A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity. J Cell Biol. 1992;119:1413-27 pubmed
    The alphoid DNA-CENP-B (centromere protein B) complex is the first sequence-specific DNA/protein complex detected in the centromeric region of human chromosomes...
  2. Okamoto Y, Nakano M, Ohzeki J, Larionov V, Masumoto H. A minimal CENP-A core is required for nucleation and maintenance of a functional human centromere. EMBO J. 2007;26:1279-91 pubmed
    ..The data presented here suggest that a functional minimum core stably maintained on 30-70 kb alphoid DNA arrays represents an epigenetic memory of centromeric chromatin. ..
  3. Politi V, Perini G, Trazzi S, Pliss A, Raska I, Earnshaw W, et al. CENP-C binds the alpha-satellite DNA in vivo at specific centromere domains. J Cell Sci. 2002;115:2317-27 pubmed
  4. Fowler K, Hudson D, Salamonsen L, Edmondson S, Earle E, Sibson M, et al. Uterine dysfunction and genetic modifiers in centromere protein B-deficient mice. Genome Res. 2000;10:30-41 pubmed
    b>Centromere protein B (CENP-B) binds constitutively to mammalian centromere repeat DNA and is highly conserved between humans and mouse. Cenpb null mice appear normal but have lower body and testis weights...
  5. Perez Castro A, Shamanski F, Meneses J, Lovato T, Vogel K, Moyzis R, et al. Centromeric protein B null mice are viable with no apparent abnormalities. Dev Biol. 1998;201:135-43 pubmed
    The centromere protein B (CENP-B) is a centromeric DNA/binding protein. It recognizes a 17-bp sequence motif called the CENP-B box, which is found in the centromeric region of most chromosomes...
  6. Halverson D, Baum M, Stryker J, Carbon J, Clarke L. A centromere DNA-binding protein from fission yeast affects chromosome segregation and has homology to human CENP-B. J Cell Biol. 1997;136:487-500 pubmed
    ..The identification of a CENP-B-related centromere DNA-binding protein in S. pombe strongly supports the hypothesis that fission yeast centromeres are structurally and functionally related to the centromeres of higher eukaryotes. ..
  7. Bejarano L, Valdivia M. Molecular cloning of an intronless gene for the hamster centromere antigen CENP-B. Biochim Biophys Acta. 1996;1307:21-5 pubmed
    b>Centromere protein B (CENP-B) is a DNA-binding protein present at both active and inactive centromeres. It was first localized at the kinetochore region by human autoimmune sera from CREST patients...
  8. Earnshaw W, Sullivan K, Machlin P, Cooke C, Kaiser D, Pollard T, et al. Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen. J Cell Biol. 1987;104:817-29 pubmed
    ..Quite unexpectedly, immunofluorescence analysis using antibodies specific for CENP-B reveals that the levels of antigen vary widely between chromosomes. ..
  9. Irelan J, Gutkin G, Clarke L. Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B. Genetics. 2001;157:1191-203 pubmed
    ..These results suggest that indirect cooperation between different CENP-B-like DNA binding proteins with partially overlapping chromatin distributions helps to establish a functional centromere. ..

More Information


  1. Tanaka Y, Tachiwana H, Yoda K, Masumoto H, Okazaki T, Kurumizaka H, et al. Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences. J Biol Chem. 2005;280:41609-18 pubmed
    ..Therefore, CENP-B may be a determinant for translational positioning of the centromere-specific nucleosomes through its binding to the nucleosomal CENP-B box. ..
  2. Schuh M, Lehner C, Heidmann S. Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase. Curr Biol. 2007;17:237-43 pubmed
    ..Thus, our findings uncover a strikingly dynamic behavior of centromere components in anaphase. ..
  3. Ando S, Yang H, Nozaki N, Okazaki T, Yoda K. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells. Mol Cell Biol. 2002;22:2229-41 pubmed
    ..On the basis of these results, we propose that the CENP-A/B/C chromatin complex is selectively formed on the I-type alpha-satellite array and constitutes the prekinetochore in HeLa cells. ..
  4. Yoda K, Nakamura T, Masumoto H, Suzuki N, Kitagawa K, Nakano M, et al. Centromere protein B of African green monkey cells: gene structure, cellular expression, and centromeric localization. Mol Cell Biol. 1996;16:5169-77 pubmed
    b>Centromere protein B (CENP-B) is a centromeric DNA-binding protein which recognizes a 17-bp sequence (CENP-B box) in human and mouse centromeric satellite DNA...
  5. Lee J, Huberman J, Hurwitz J. Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe. Proc Natl Acad Sci U S A. 1997;94:8427-32 pubmed
    ..Based on its binding activity to centromeric DNA and homology to centromere proteins, we suggest that this protein may be a functional homologue of the mammalian CENP-B in S. pombe. ..
  6. Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S. A helix-turn-helix structure unit in human centromere protein B (CENP-B). EMBO J. 1998;17:827-37 pubmed
    ..The results suggest that CENP-B DBD RP1 interacts with one of the essential regions of the CENP-B box DNA, mainly at the N-terminal basic region, the N-terminal portion of helix 2 and helix 3. ..
  7. Okada T, Ohzeki J, Nakano M, Yoda K, Brinkley W, Larionov V, et al. CENP-B controls centromere formation depending on the chromatin context. Cell. 2007;131:1287-300 pubmed
    ..Thus, we propose that CENP-B plays a dual role in centromere formation, ensuring de novo formation on DNA lacking a functional centromere but preventing the formation of excess centromeres on chromosomes. ..
  8. Cooke C, Bernat R, Earnshaw W. CENP-B: a major human centromere protein located beneath the kinetochore. J Cell Biol. 1990;110:1475-88 pubmed
    ..This difference in staining pattern may reflect the presence of a "collar" of chromatin rich in CENP-C and/or CENP-A encircling the kinetochore plates. ..
  9. Masumoto H, Ikeno M, Nakano M, Okazaki T, Grimes B, Cooke H, et al. Assay of centromere function using a human artificial chromosome. Chromosoma. 1998;107:406-16 pubmed
    ..We conclude that alpha21-I alphoid DNA can induce de novo assembly of active centromere/kinetochore structures on minichromosomes. ..
  10. Hemmerich P, Weidtkamp Peters S, Hoischen C, Schmiedeberg L, Erliandri I, Diekmann S. Dynamics of inner kinetochore assembly and maintenance in living cells. J Cell Biol. 2008;180:1101-14 pubmed publisher
    ..Our data reveal a wide range of cell cycle-specific assembly plasticity of the centromere that provides both stability through sustained binding of some components and flexibility through dynamic exchange of other components. ..
  11. Mahler M, Mierau R, Genth E, Bluthner M. Development of a CENP-A/CENP-B-specific immune response in a patient with systemic sclerosis. Arthritis Rheum. 2002;46:1866-72 pubmed
    ..Whether the D-penicillamine treatment received by this patient was involved in the triggering of this cascade remains a matter of speculation. ..
  12. Howman E, Fowler K, Newson A, Redward S, MacDonald A, Kalitsis P, et al. Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. Proc Natl Acad Sci U S A. 2000;97:1148-53 pubmed
    ..The evidence is consistent with the proposal of a critical epigenetic function for CENP-A in marking a chromosomal region for centromere formation. ..
  13. Van Hooser A, Ouspenski I, Gregson H, Starr D, Yen T, Goldberg M, et al. Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A. J Cell Sci. 2001;114:3529-42 pubmed
  14. Tudor M, Lobocka M, Goodell M, Pettitt J, O Hare K. The pogo transposable element family of Drosophila melanogaster. Mol Gen Genet. 1992;232:126-34 pubmed
    ..Homologous sequences were not detected in other Drosophila species, suggesting that this transposable element family may be restricted to D. melanogaster. ..
  15. Wang I, Chen Y, Hughes D, Petrovic V, Major M, Park H, et al. Forkhead box M1 regulates the transcriptional network of genes essential for mitotic progression and genes encoding the SCF (Skp2-Cks1) ubiquitin ligase. Mol Cell Biol. 2005;25:10875-94 pubmed
    ..Taken together, these results demonstrate that FoxM1 regulates transcription of cell cycle genes critical for progression into S-phase and mitosis. ..
  16. Pluta A, Saitoh N, Goldberg I, Earnshaw W. Identification of a subdomain of CENP-B that is necessary and sufficient for localization to the human centromere. J Cell Biol. 1992;116:1081-93 pubmed
    ..Analysis of the distribution of this sequence in alpha-satellite DNA suggests that CENP-B binding may have profound effects on chromatin structure at centromeres. ..
  17. Cam H, Noma K, Ebina H, Levin H, Grewal S. Host genome surveillance for retrotransposons by transposon-derived proteins. Nature. 2008;451:431-6 pubmed
  18. Hudson D, Fowler K, Earle E, Saffery R, Kalitsis P, Trowell H, et al. Centromere protein B null mice are mitotically and meiotically normal but have lower body and testis weights. J Cell Biol. 1998;141:309-19 pubmed
    ..A model for a functional redundancy of this protein is presented. ..
  19. Kitagawa K, Masumoto H, Ikeda M, Okazaki T. Analysis of protein-DNA and protein-protein interactions of centromere protein B (CENP-B) and properties of the DNA-CENP-B complex in the cell cycle. Mol Cell Biol. 1995;15:1602-12 pubmed
    We previously reported that centromere protein B (CENP-B) forms a stable complex (designated complex A) containing two alphoid DNAs in vitro...
  20. Smit A, Riggs A. Tiggers and DNA transposon fossils in the human genome. Proc Natl Acad Sci U S A. 1996;93:1443-8 pubmed
    ..We further identified relatively low-copy-number mariner elements in both human and sheep DNA. These belong to two subfamilies previously identified in insect genomes, suggesting lateral transfer between diverse species. ..
  21. Akbarali Y, Matousek Ronck J, Hunt L, Staudt L, Reichlin M, Guthridge J, et al. Fine specificity mapping of autoantigens targeted by anti-centromere autoantibodies. J Autoimmun. 2006;27:272-80 pubmed
    ..This epitope mapping study identifies key antigenic targets of the anti-centromere response and establishes that the majority of the responses depend on key amino-terminal residues. ..
  22. Suzuki N, Nakano M, Nozaki N, Egashira S, Okazaki T, Masumoto H. CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization. J Biol Chem. 2004;279:5934-46 pubmed
    ..In other words, a possible molecular linkage may exist between one of the kinetochore components and human centromere DNA through CENP-B/CENP-B box interaction. ..
  23. Tawaramoto M, Park S, Tanaka Y, Nureki O, Kurumizaka H, Yokoyama S. Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution. J Biol Chem. 2003;278:51454-61 pubmed
    The human centromere protein B (CENP-B), a centromeric heterochromatin component, forms a homodimer that specifically binds to a distinct DNA sequence (the CENP-B box), which appears within every other alpha-satellite repeat...
  24. Orthaus S, Biskup C, Hoffmann B, Hoischen C, Ohndorf S, Benndorf K, et al. Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells. Chembiochem. 2008;9:77-92 pubmed
    ..On the other hand, no FRET was detected between CENP-A and H2A.1 or H3.1. Our data support the view that two CENP-A molecules are packed with H4, but not with H3, in a single centromeric nucleosome. ..
  25. Aguilar Arnal L, Marsellach F, Azorín F. The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching. EMBO J. 2008;27:1029-38 pubmed publisher
    ..Cbh1 and Cbh2, which are also homologous to CENP-B, have only a minor contribution to the regulation of directionality of switching, which is in contrast with the strong effects observed for Abp1. ..
  26. Kapoor M, Montes de Oca Luna R, Liu G, Lozano G, Cummings C, Mancini M, et al. The cenpB gene is not essential in mice. Chromosoma. 1998;107:570-6 pubmed
    b>Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence...
  27. Sugimoto K, Yata H, Himeno M. Mapping of the human CENP-B gene to chromosome 20 and the CENP-C gene to chromosome 12 by a rapid cycle DNA amplification procedure. Genomics. 1993;17:240-2 pubmed
    ..Using a panel of rodent/human hybrid DNA, the genes for human CENP-B and CENP-C were conveniently mapped to chromosomes 20 and 12, respectively. ..
  28. Gelber A, Pillemer S, Baum B, Wigley F, Hummers L, Morris S, et al. Distinct recognition of antibodies to centromere proteins in primary Sjogren's syndrome compared with limited scleroderma. Ann Rheum Dis. 2006;65:1028-32 pubmed
    ..These findings suggest that obtaining antibodies to specific centromere antigens is useful diagnostically, and imply that distinct mechanisms underlie the unique patterns of centromere autoreactivity in pSS and scleroderma. ..
  29. Lorenz D, Mikheyeva I, Johansen P, Meyer L, Berg A, Grewal S, et al. CENP-B cooperates with Set1 in bidirectional transcriptional silencing and genome organization of retrotransposons. Mol Cell Biol. 2012;32:4215-25 pubmed
    ..Our study illuminates a molecular pathway involving a transposase-containing transcription factor that cooperates with chromatin modifiers to regulate TE activities. ..
  30. Stahnke G, Meier E, Scanarini M, Northemann W. Eukaryotic expression of recombinant human centromere autoantigen and its use in a novel ELISA for diagnosis of CREST syndrome. J Autoimmun. 1994;7:107-18 pubmed
    The cDNA coding for the human full-length centromere protein B (CENP-B) was isolated from a liver cDNA library by oligonucleotide screening and extended at the 5' and 3' ends by linker addition...
  31. Seki N, Saito T, Kitagawa K, Masumoto H, Okazaki T, Hori T. Mapping of the human centromere protein B gene (CENPB) to chromosome 20p13 by fluorescence in situ hybridization. Genomics. 1994;24:187-8 pubmed
  32. Tomonaga T, Matsushita K, Yamaguchi S, Oohashi T, Shimada H, Ochiai T, et al. Overexpression and mistargeting of centromere protein-A in human primary colorectal cancer. Cancer Res. 2003;63:3511-6 pubmed
    ..These results suggest that overexpression of CENP-A could play an important role for aneuploidy in colorectal cancers. ..
  33. Ohzeki J, Nakano M, Okada T, Masumoto H. CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA. J Cell Biol. 2002;159:765-75 pubmed
    ..To the best of our knowledge, this is the first reported evidence of a functional molecular link between a centromere-specific DNA sequence and centromeric chromatin assembly in humans. ..
  34. Sullivan K, Glass C. CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins. Chromosoma. 1991;100:360-70 pubmed
    ..These data demonstrate that CENP-B is a highly conserved mammalian protein that may be a member of the HLH protein family and suggest that it plays a role in a conserved aspect of centromere structure or function. ..
  35. Baum M, Clarke L. Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation. Mol Cell Biol. 2000;20:2852-64 pubmed
    ..The relevance of these findings to mammalian centromeres is discussed. ..
  36. Wente M, Jain A, Kono E, Berberat P, Giese T, Reber H, et al. Prostate stem cell antigen is a putative target for immunotherapy in pancreatic cancer. Pancreas. 2005;31:119-25 pubmed
    ..These results show a potential therapeutic role for anti-PSCA antibodies in the treatment of pancreatic cancer. Furthermore, PSCA might serve as a novel marker in the diagnosis of pancreatic cancer. ..
  37. Sugimoto K, Fukuda R, Himeno M. Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. Cell Struct Funct. 2000;25:253-61 pubmed
    ..These cell lines will further be used for visualizing the prekinetochore locus in interphase nuclei as well as analyzing kinetochore dynamics in the living cells. ..
  38. Tian A, Benchabane H, Wang Z, Zimmerman C, Xin N, Perochon J, et al. Intestinal stem cell overproliferation resulting from inactivation of the APC tumor suppressor requires the transcription cofactors Earthbound and Erect wing. PLoS Genet. 2017;13:e1006870 pubmed publisher
    ..Such differentially utilized transcription cofactors may offer new opportunities for the selective targeting of Wnt-driven cancers. ..
  39. Low A, Wong S, Thumboo J, Ng S, Lim J, Ng X, et al. Evaluation of a new multi-parallel line immunoassay for systemic sclerosis-associated antibodies in an Asian population. Rheumatology (Oxford). 2012;51:1465-70 pubmed publisher
    ..00) (P?<?0.05). Anti-Ro60 was associated with pulmonary arterial hypertension (OR 3.89, P?=?0.041). The SSc-LIA has good agreement with conventional techniques for selected antibodies and has good diagnostic utility. ..
  40. Benchabane H, Xin N, Tian A, Hafler B, Nguyen K, Ahmed A, et al. Jerky/Earthbound facilitates cell-specific Wnt/Wingless signalling by modulating β-catenin-TCF activity. EMBO J. 2011;30:1444-58 pubmed publisher
    ..These findings indicate that tissue-restricted transcriptional co-activators facilitate cell-specific Wnt/Wingless signalling responses by modulating β-catenin-TCF activity. ..
  41. Okouneva T, Azarenko O, Wilson L, Littlefield B, Jordan M. Inhibition of centromere dynamics by eribulin (E7389) during mitotic metaphase. Mol Cancer Ther. 2008;7:2003-11 pubmed publisher
    ..The strong correlation between suppression of kinetochore-microtubule dynamics and mitotic arrest indicates that the primary mechanism by which eribulin blocks mitosis is suppression of spindle microtubule dynamics. ..
  42. Reddy K, Sulcova V, Schwartz S, Noble J, Phillips J, Brasel J, et al. Mosaic tetrasomy 8q: inverted duplication of 8q23.3qter in an analphoid marker. Am J Med Genet. 2000;92:69-76 pubmed
    ..3qter. The parents had normal karyotypes. Centromeric proteins CENP-C and CENP-E were present, but alpha associated centromere protein CENP-B was absent at a position defining a neocentromere. ..
  43. Mashkova T, Oparina N, Lacroix M, Fedorova L, G Tumeneva I, Zinovieva O, et al. Structural rearrangements and insertions of dispersed elements in pericentromeric alpha satellites occur preferably at kinkable DNA sites. J Mol Biol. 2001;305:33-48 pubmed
    ..It seems that kinkable dinucleotides TG, CA and TA are part of recognition signals for many proteins involved in recombination, replication, and insertional events. Alphoid DNA is a good model for studying these processes. ..
  44. Kuznetsova I, Matveev I, Podgornaia O, Enukashvili N. [Position of the satellite DNA relative to heterochromatin in the interphase nuclei in cultured cells]. Tsitologiia. 2002;44:422-30 pubmed
  45. Dol nik A, Luk ianov D, Enukashvili N, Podgornaia O. [Localization of proteins, specifically binding highly-repetitive sequences of DNA in human spermatozoids]. Tsitologiia. 2001;43:681-91 pubmed
    Immunoblot revealed in spermatozoa alpha-satellite (sat) DNA-specific centromere protein B (CENP-B) and p70 (Enukashvily et al., 2000), a membrane telomere binding protein (MTBP/TRF2) (Podgornaya et al...
  46. Lomonte P, Morency E. Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0. FEBS Lett. 2007;581:658-62 pubmed
    ..CENP-B is one of the major proteins of centromeres and its degradation is likely to contribute to the severe damage induced to centromeres by ICP0. ..
  47. Dawe R. RNA interference, transposons, and the centromere. Plant Cell. 2003;15:297-301 pubmed
  48. Ma W, Zhang D, Hou Y, Li Y, Sun Q, Sun X, et al. Reduced expression of MAD2, BCL2, and MAP kinase activity in pig oocytes after in vitro aging are associated with defects in sister chromatid segregation during meiosis II and embryo fragmentation after activation. Biol Reprod. 2005;72:373-83 pubmed
    This study was conducted to examine expression of centromere protein B (CENPB), spindle checkpoint protein MAD2 (mitotic arrest deficient protein), and antiapoptotic protein BCL2; activities of MAPK (mitogen-activated protein kinase) and ..
  49. Bizzaro N, Bonelli F, Tonutti E, Villalta D, Tozzolis R. Autoantibody detection in scleroderma patients. Diagnostic and analytical performances of a new coupled particle light scattering immunoassay. Clin Exp Rheumatol. 2002;20:45-51 pubmed
    ..The results of this study demonstrate that the light scattering technology is also applicable to the detection of autoantibodies to intracellular antigens for the diagnosis of autoimmune rheumatic diseases. ..
  50. Hanke K, Becker M, Brueckner C, Meyer W, Janssen A, Schlumberger W, et al. Anticentromere-A and anticentromere-B antibodies show high concordance and similar clinical associations in patients with systemic sclerosis. J Rheumatol. 2010;37:2548-52 pubmed publisher
    ..Detection of both antibodies in parallel may slightly increase the diagnostic sensitivity for SSc. ..
  51. Saxena A, Saffery R, Wong L, Kalitsis P, Choo K. Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated. J Biol Chem. 2002;277:26921-6 pubmed
  52. Fachinetti D, Folco H, Nechemia Arbely Y, Valente L, Nguyen K, Wong A, et al. A two-step mechanism for epigenetic specification of centromere identity and function. Nat Cell Biol. 2013;15:1056-66 pubmed publisher
  53. Kato H, Murakami Y. [Heterochromatin and centromere: heterochromatin meets RNAi]. Tanpakushitsu Kakusan Koso. 2004;49:1990-7 pubmed