acholeplasma

Summary

Summary: A genus of gram-negative organisms including saprophytic and parasitic or pathogenic species.

Top Publications

  1. Knight T. Reclassification of Mesoplasma pleciae as Acholeplasma pleciae comb. nov. on the basis of 16S rRNA and gyrB gene sequence data. Int J Syst Evol Microbiol. 2004;54:1951-2 pubmed
    ..gene of Mesoplasma pleciae PS-1(T) (=ATCC 49582(T)=NBRC 100476(T)) demonstrate a much closer relationship to Acholeplasma laidlawii and Acholeplasma oculi than to other species in the order Entomoplasmatales...
  2. Knudtson K, Minion F. Use of lac gene fusions in the analysis of Acholeplasma upstream gene regulatory sequences. J Bacteriol. 1994;176:2763-6 pubmed
    ..Analysis of Acholeplasma oculi ISM1499 transcriptional sequences was accomplished using a promoterless lacZ reporter gene and an ..
  3. Pollack J, Williams M, Banzon J, Jones M, Harvey L, Tully J. Comparative metabolism of Mesoplasma, Entomoplasma, Mycoplasma, and Acholeplasma. Int J Syst Bacteriol. 1996;46:885-90 pubmed
    Cytoplasmic fractions from species of the Mollicutes genera Entomoplasma, Mesoplasma, Mycoplasma, and Acholeplasma were assayed for NADH oxidase (NADH ox), ATP- and PPi-dependent phosphofructokinase (PFK), ATP- and PPi-dependent ..
  4. Angulo A, Reijgers R, Brugman J, Kroesen I, Hekkens F, Carle P, et al. Acholeplasma vituli sp. nov., from bovine serum and cell cultures. Int J Syst Evol Microbiol. 2000;50 Pt 3:1125-31 pubmed
    ..The organism was serologically distinct from all 13 currently described species in the genus Acholeplasma and from other sterol-requiring species in the genus Mycoplasma, using growth inhibition, immunoperoxidase and ..
  5. Volokhov D, Neverov A, George J, Kong H, Liu S, Anderson C, et al. Genetic analysis of housekeeping genes of members of the genus Acholeplasma: phylogeny and complementary molecular markers to the 16S rRNA gene. Mol Phylogenet Evol. 2007;44:699-710 pubmed
    ..as the complete sequence of the 16S-23S rRNA intergenic transcribed spacer (ITS) were determined for all known Acholeplasma species...
  6. Just W, da Silva Cardoso M, Lorenz A, Klotz G. Release of mycoplasmavirus L1 upon transfection of Acholeplasma laidlawii with homologous and heterologous viral DNA. Arch Virol. 1989;107:1-13 pubmed
    This communication reports on the release of Mycoplasmavirus L1 after infection of Acholeplasma laidlawii with purified L3 virus. Release also occurred after transfection with certain restriction fragments from MV-L3 and MV-L1 genomes...
  7. Al Farha A, Hemmatzadeh F, Khazandi M, Hoare A, Petrovski K. Evaluation of effects of Mycoplasma mastitis on milk composition in dairy cattle from South Australia. BMC Vet Res. 2017;13:351 pubmed publisher
    ..components has been widely addressed in the literature, limited data on the effects of different Mycoplasma and Acholeplasma spp. on milk quality and quantity is available...
  8. Abendroth C, Hahnke S, Simeonov C, Klocke M, Casani Miravalls S, Ramm P, et al. Microbial communities involved in biogas production exhibit high resilience to heat shocks. Bioresour Technol. 2018;249:1074-1079 pubmed publisher
    ..Bacteroides and Acholeplasma were reduced after heat shocks. An increase was observed for Aminobacterium...
  9. Helling A, König H, Seiler F, Berkholz R, Thom V, Polakovic M. Retention of Acholeplasma laidlawii by Sterile Filtration Membranes: Effect of Cultivation Medium and Filtration Temperature. PDA J Pharm Sci Technol. 2018;72:264-277 pubmed publisher
    ..size, zeta potential, and the ability to penetrate tailor-made size exclusion membrane filters of mycoplasma Acholeplasma laidlawii cultivated in five different cultivation media...

More Information

Publications77

  1. Medvedeva E, Siniagina M, Malanin S, Boulygina E, Malygina T, Baranova N, et al. Genome Sequences of Acholeplasma laidlawii Strains Differing in Sensitivity to Ciprofloxacin. Genome Announc. 2017;5: pubmed publisher
    i>Acholeplasma laidlawii is a well-suited model for study of the molecular basis of the adaptation of mollicutes to environmental conditions. Here we present the whole-genome sequences of four strains of A...
  2. Matsuoka S. Biological functions of glucolipids in Bacillus subtilis. Genes Genet Syst. 2017;: pubmed publisher
    ..the expression of monoglucosyldiacylglycerol (MGlcDG) by 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii (alMGS) almost completely suppresses the ugtP disruptant phenotype...
  3. Jia S, Zhang X, Miao Y, Zhao Y, Ye L, Li B, et al. Fate of antibiotic resistance genes and their associations with bacterial community in livestock breeding wastewater and its receiving river water. Water Res. 2017;124:259-268 pubmed publisher
    ..The genera of Treponema, Prevotella, Pseudomonas, Bacteroides, Oscillibacter and Acholeplasma dominated in the wastewater samples and almost disappeared in the receiving river water, but bacterial ..
  4. Siqueira F, Cibulski S, Teixeira T, Mayer F, Roehe P. Draft Genome Sequence of Acholeplasma laidlawii, a Common Contaminant of Cell Cultures. Genome Announc. 2017;5: pubmed publisher
    ..b>Acholeplasma laidlawii is one of the most frequent contaminants of cell cultures...
  5. Al Farha A, Petrovski K, Jozani R, Hoare A, Hemmatzadeh F. Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis. BMC Res Notes. 2018;11:107 pubmed publisher
    ..resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single commercial dairy farm in South Australia...
  6. Kalshingi H, Bosman A, Gouws J, van Vuuren M. Molecular characterisation of Mycoplasma species isolated from the genital tract of Dorper sheep in South Africa. J S Afr Vet Assoc. 2015;86:e1-e11 pubmed publisher
    ..sp. ovine/caprine sero group II from the United Kingdom. BLAST for two isolates revealed 99% similarity to Acholeplasma laidlawii from India, another two were 99% similar to MmmLC strain from Sweden, two showed 98% similarity to ..
  7. Folmsbee M, Lentine K, Wright C, Haake G, Mcburnie L, Ashtekar D, et al. The Development of a Microbial Challenge Test with Acholeplasma laidlawii To Rate Mycoplasma-Retentive Filters by Filter Manufacturers. PDA J Pharm Sci Technol. 2014;68:281-96 pubmed publisher
  8. Tokovenko I, Malynovs ka L. [Amino acid assimilation by representatives of the Acholeplasma genus]. Mikrobiol Z. 2005;67:9-14 pubmed
    Amino acid assimilation by different representatives of Acholeplasma genus has been investigated. It was shown that all 7 investigated typical strains Acholeplasma laidlawii PG-8, A. granularum BTS-39, A. modicum PG-49, A. oculi 19-L, A...
  9. Lim P, Sears B. The genome size of a plant-pathogenic mycoplasmalike organism resembles those of animal mycoplasmas. J Bacteriol. 1991;173:2128-30 pubmed
    ..Its genome size was similar to that of Mycoplasma gallisepticum and much smaller than that of Acholeplasma laidlawii...
  10. Wang J, Johnson S, Brown M, Lute S, Agarabi C, Dabrazhynetskaya A, et al. Mycoplasma Clearance and Risk Analysis in a Model Bioprocess. PDA J Pharm Sci Technol. 2017;71:99-114 pubmed publisher
    ..of typical commercial monoclonal antibody purification operations to clear and kill mycoplasmas, using Acholeplasma laidlawii as a model organism...
  11. Polianskaia G, Samokish V. [The study of quantitative karyotypic variability by induction of chromosomal instability in cultured cells of the Indian muntjac skin fibroblasts]. Tsitologiia. 1999;41:752-7 pubmed
    ..Mycoplasma arginini and Acholeplasma laidlawii, used as factors inducing chromosomal instability, do not break the main regulations peculiar to ..
  12. Liljander A, Yu M, O Brien E, Heller M, Nepper J, Weibel D, et al. Field-Applicable Recombinase Polymerase Amplification Assay for Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae. J Clin Microbiol. 2015;53:2810-5 pubmed publisher
    ..strain ILRI181, while no amplification was obtained from 71 related Mycoplasma isolates or from the Acholeplasma or the Pasteurella isolates, demonstrating a high degree of specificity...
  13. Mantsch H, Yang P, Martin A, Cameron D. Infrared spectroscopic studies of Acholeplasma laidlawaii B membranes. Comparison of the gel to liquid-crystal phase transition in intact cells and isolated membranes. Eur J Biochem. 1988;178:335-41 pubmed
    The physical state of the membrane lipids in the plasma membranes of intact, live Acholeplasma laidlawii B cells was probed by Fourier-transform infrared spectroscopy and compared with that in isolated membranes...
  14. Tully J, Rose D, Whitcomb R, Hackett K, Clark T, Henegar R, et al. Characterization of some new insect-derived acholeplasmas. Isr J Med Sci. 1987;23:699-703 pubmed
    ..Mollicutes (strains PS-1, TAC, and YJS) isolated from gut fluids of insects confirms their similarity to Acholeplasma. They are serologically distinct from acholeplasmas of vertebrates and several other sterol nonrequiring ..
  15. Koui T, Natsuaki T, Okuda S. Antiserum raised against gyrase A of Acholeplasma laidlawii reacts with phytoplasma proteins. FEMS Microbiol Lett. 2002;206:169-74 pubmed
    Part of the gyrase A gene (gyrA) of Acholeplasma laidlawii was cloned and incorporated directly downstream from a 6 x His tag segment of the pQE expression vector...
  16. Le Grand D, Saras E, Blond D, Solsona M, Poumarat F. Assessment of PCR for routine identification of species of the Mycoplasma mycoides cluster in ruminants. Vet Res. 2004;35:635-49 pubmed
    ..The occurrence of putative variable antigen(s) on the mycoplasma surface may explain part of the identification difficulties encountered with the immunological methods...
  17. Abu Amero K, Abu Groun E, Halablab M, Miles R. Kinetics and distribution of alcohol oxidising activity in Acholeplasma and Mycoplasma species. FEMS Microbiol Lett. 2000;183:147-51 pubmed
    Alcohol metabolism by Acholeplasma and Mycoplasma cell suspensions was determined using changes in dissolved oxygen tension to monitor oxygen uptake...
  18. Manolukas J, Barile M, Chandler D, Pollack J. Presence of anaplerotic reactions and transamination, and the absence of the tricarboxylic acid cycle in mollicutes. J Gen Microbiol. 1988;134:791-800 pubmed
    Cell extracts of the fermentative Mollicutes Acholeplasma laidlawii B-PG9, Acholeplasma morum S2, Mycoplasma capricolum 14, Mycoplasma gallisepticum S6, Mycoplasma pneumoniae FH, Mycoplasma hyopneumoniae J and M...
  19. Shahhosseiny M, Hosseiny Z, Khoramkhorshid H, Azari S, Shokrgozar M. Rapid and sensitive detection of Mollicutes in cell culture by polymerase chain reaction. J Basic Microbiol. 2010;50:171-8 pubmed publisher
    Infections with Mollicutes species (such as Mycoplasma, Acholeplasma, and Ureaplasma) can induce a variety of problems in living organisms and laboratory cell cultures...
  20. Economopoulos K, Ward N, Phillips C, Teshager A, Patel P, Mohamed M, et al. Prevention of antibiotic-associated metabolic syndrome in mice by intestinal alkaline phosphatase. Diabetes Obes Metab. 2016;18:519-27 pubmed publisher
    ..To examine whether co-administration of intestinal alkaline phosphatase (IAP) with antibiotics early in life may have a preventive role against metabolic syndrome (MetS) in mice...
  21. Meier B, Habermehl G. Evidence for superoxide dismutase and catalase in mollicutes and release of reactive oxygen species. Arch Biochem Biophys. 1990;277:74-9 pubmed
    ..Anaerobic mycoplasmas showed the highest amount of radical production, whereas superoxide dismutase and catalase activities were in the range of activities estimated for aerobic mollicutes...
  22. Artiushin S, DuVall M, Minion F. Phylogenetic analysis of mycoplasma strain ISM1499 and its assignment to the Acholeplasma oculi strain cluster. Int J Syst Bacteriol. 1995;45:104-9 pubmed
    ..position of ISM1499, and on the basis of the results of our genetic analysis, this strain was assigned to the Acholeplasma oculi strain cluster. The base composition of strain ISM1499 was identical to the base composition of A...
  23. Tully J, Whitcomb R, Rose D, Bove J, Carle P, Somerson N, et al. Acholeplasma brassicae sp. nov. and Acholeplasma palmae sp. nov., two non-sterol-requiring mollicutes from plant surfaces. Int J Syst Bacteriol. 1994;44:680-4 pubmed
    ..The two isolates were not serologically related to each other or to the type strains of 11 previously described Acholeplasma species. Strain 0502 (= ATCC 49388) is the type strain of Acholeplasma brassicae sp. nov...
  24. Navas Castillo J, Laigret F, Tully J, Bove J. [Mollicute Acholeplasma florum possesses a gene of phosphoenolpyruvate sugar phosphotransferase system and it uses UGA as tryptophan codon]. C R Acad Sci III. 1992;315:43-8 pubmed
    ..b>Acholeplasma florum, because of its metabolic properties and its close phylogenetic relationship to members of the genera ..
  25. Sundaram S, Eisenhuth J, Lewis M, Howard G, Brandwein H. Method for qualifying microbial removal performance of 0.1 micron rated filters. Part III: bacterial challenge tests on 0.2/0.22 and 0.1 micron rated filter cartridges with Hydrogenophaga (formerly Pseudomonas) pseudoflava. PDA J Pharm Sci Technol. 2001;55:393-416 pubmed
    ..This study demonstrates that H. pseudoflava meets all criteria for use as a standard organism for qualifying the microbial removal performance of 0.1 microm rated filters for enhanced sterility assurance...
  26. Lee I, Gundersen Rindal D, Davis R, Bottner K, Marcone C, Seemuller E. 'Candidatus Phytoplasma asteris', a novel phytoplasma taxon associated with aster yellows and related diseases. Int J Syst Evol Microbiol. 2004;54:1037-48 pubmed
    ..AY subclade strains are related more closely to their culturable relatives, Acholeplasma spp., than any other phytoplasmas known...
  27. Ayling R, Bashiruddin S, Nicholas R. Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet Rec. 2004;155:413-6 pubmed
    ..Mycoplasma bovirhinis and Acholeplasma species were found commonly but are thought to be more opportunistic than pathogenic...
  28. Furneri P, Tempera G, Bisignano G, Cottarelli F, Nicoletti G. Anti-mycoplasmal activity of a new macrolide: miocamycin. Chemioterapia. 1987;6:341-5 pubmed
    ..activity of miocamycin and other macrolides (erythromycin and josamycin) against 61 Ureaplasma urealyticum, 1 Acholeplasma laidlawii and 21 aerobe mycoplasmas (M. pneumoniae, M. hominis, M. gallisepticum, M...
  29. Jang H, Kim H, Kang B, Kim C, Park H. Oligonucleotide array-based detection and genotyping of mollicutes (Acholeplasma, Mycoplasma, and Ureaplasma). J Microbiol Biotechnol. 2009;19:265-70 pubmed
    ..proposed oligonucleotide array was effective for the detection and discrimination of 23 species, including an acholeplasma, 21 mycoplasmas, and a ureaplasma, and showed promise as a countermeasure to ensure that biological products ..
  30. Navas Castillo J, Laigret F, Hocquellet A, Chang C, Bove J. Evidence for a phosphoenolpyruvate dependent sugar-phosphotransferase system in the mollicute Acholeplasma florum. Biochimie. 1993;75:675-9 pubmed
    ..the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose ..
  31. Boonyayatra S, Fox L, Gay J, Sawant A, Besser T. Discrimination between Mycoplasma and Acholeplasma species of bovine origin using digitonin disc diffusion assay, nisin disc diffusion assay, and conventional polymerase chain reaction. J Vet Diagn Invest. 2012;24:7-13 pubmed publisher
    ..is effective in isolating mollicutes that are Mycoplasma-like; however, isolates may be misinterpreted as Acholeplasma species, which are indistinguishable from Mycoplasma species by culture...
  32. Stipkovits L, Nicolet J, Haldimann A, Frey J. Use of antibodies against the P36 protein of Mycoplasma hyopneumoniae for the identification of M. hyopneumoniae strains. Mol Cell Probes. 1991;5:451-7 pubmed
    ..In addition, we have found no reaction with anti-P36 antibodies using 47 different Mycoplasma or Acholeplasma species isolated from human, mice, rat, poultry, ruminant, dog and cat...
  33. Poddar S, Maniloff J. Determination of microbial genome sizes by two-dimensional denaturing gradient gel electrophoresis. Nucleic Acids Res. 1989;17:2889-95 pubmed
    ..This method has been applied to calculate three microbial genome sizes: Mycoplasma capricolum, 724 kb; Acholeplasma laidlawii, 1646 kb; and Hemophilus influenzae, 1833 kb.
  34. Tiong S. Mycoplasmas and acholeplasmas isolated from ducks and their possible association with pasteurellas. Vet Rec. 1990;127:64-6 pubmed
    ..columbinasale, two M gallinaceum, two M gallinarum, nine M synoviae, three unidentified Mycoplasma species, 37 Acholeplasma laidlawii and one unclassified acholeplasma belonging to each of serogroups 7 and 8...
  35. Egwu G, Faull W, Bradbury J, Clarkson M. Ovine infectious keratoconjunctivitis: a microbiological study of clinically unaffected and affected sheep's eyes with special reference to Mycoplasma conjunctivae. Vet Rec. 1989;125:253-6 pubmed
    ..The genera Mycoplasma and Acholeplasma were isolated from both groups, and Mycoplasma conjunctivae occurred in 92 affected eyes (38...
  36. Hill A. Acholeplasma cavigenitalium sp. nov., isolated from the vagina of guinea pigs. Int J Syst Bacteriol. 1992;42:589-92 pubmed
    ..from a guinea pig vagina was shown to be serologically distinct from previously recognized Mycoplasma and Acholeplasma species. Colonies isolated from 10 different guinea pigs were cloned and examined in detail...
  37. Salling H, Bang Christensen S. Multi-primer qPCR assay capable of highly efficient and specific detection of the vast majority of all known Mycoplasma. Biologicals. 2016;44:129-38 pubmed publisher
    ..The assay will in all probability detect at least 356 species and strains of Mycoplasma, Spiroplasma and Acholeplasma with high sensitivity...
  38. Sundaram S, Eisenhuth J, Howard G, Brandwein H. Method for qualifying microbial removal performance of 0.1 micron rated filters. Part I: characterization of water isolates for potential use as standard challenge organisms to qualify 0.1 micron rated filters. PDA J Pharm Sci Technol. 2001;55:346-72 pubmed
    ..1 microm rated hydrophilic PVDF (polyvinylidenefluoride) filter with a specified high titer reduction claim for Acholeplasma laidlawii. In order to ensure selection of the most stable penetrative phenotype (i.e...
  39. Gundersen D, Lee I, Rehner S, Davis R, Kingsbury D. Phylogeny of mycoplasmalike organisms (phytoplasmas): a basis for their classification. J Bacteriol. 1994;176:5244-54 pubmed
    ..of the class Mollicutes and revealed that MLOs form a large discrete monophyletic clade, paraphyletic to the Acholeplasma species, within the Anaeroplasma clade...
  40. Tully J. Current status of the mollicute flora of humans. Clin Infect Dis. 1993;17 Suppl 1:S2-9 pubmed
    ..As our understanding of the human mollicute flora increases, diagnostic and clinical advances should permit the identification and control of those species that are significant pathogens in human disease...
  41. Hill A, Polak Vogelzang A, Angulo A. Acholeplasma multilocale sp. nov., isolated from a horse and a rabbit. Int J Syst Bacteriol. 1992;42:513-7 pubmed
    b>Acholeplasma strains were isolated from the nasopharynx of a horse (strain PN525T [T = type strain]) and the feces of a rabbit (strain B1). One clone of strain PN525T and one clone of strain B1 were examined in detail...
  42. Trachtenberg S. Mollicutes-wall-less bacteria with internal cytoskeletons. J Struct Biol. 1998;124:244-56 pubmed
    The structure and motility of the Mollicutes (Spiroplasma, Mycoplasma, and Acholeplasma) are briefly reviewed. The data are presented from the perspective of prokaryotic and eukaryotic motors, cytoskeletons, and cell motility...
  43. Kapke P, Knudtson K, Minion F. Transformation of Mollicutes with single-stranded Tn4001 DNA. Plasmid. 1994;32:85-8 pubmed
    b>Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001...
  44. ter Laak E, Noordergraaf J, Boomsluiter E. The nasal mycoplasmal flora of healthy calves and cows. Zentralbl Veterinarmed B. 1992;39:610-6 pubmed
    ..canis in 1 (3%) calf; M. equirhinis in 2 (1%) cows; M. conjunctivae in 2 (1%) cows; Acholeplasma laidlawii in 1 (3%) calf and 3 (1%) cows; and A. axanthum in 7 (3%) cows...
  45. Medvedeva E, Davydova M, Mouzykantov A, Baranova N, Grigoreva T, Siniagina M, et al. Genomic and proteomic profiles of Acholeplasma laidlawii strains differing in sensitivity to ciprofloxacin. Dokl Biochem Biophys. 2016;466:23-7 pubmed publisher
  46. Brown D, McLaughlin G, Brown M. Taxonomy of the feline mycoplasmas Mycoplasma felifaucium, Mycoplasma feliminutum, Mycoplasma felis, Mycoplasma gateae, Mycoplasma leocaptivus, Mycoplasma leopharyngis, and Mycoplasma simbae by 16S rRNA gene sequence comparisons. Int J Syst Bacteriol. 1995;45:560-4 pubmed
    ..phylogenetic group of mollicutes, and Mycoplasma feliminutum was positioned in the phylogenetically distant Acholeplasma group of mollicutes, showing that host family preference does not necessarily derive from bacterial ..
  47. Taylor Robinson D, Furr P. Genital mycoplasma infections. Wien Klin Wochenschr. 1997;109:578-83 pubmed
    Since 1937, 13 Mycoplasma species, two Acholeplasma species, and one Ureaplasma species have been isolated from humans...
  48. Nims R, Gauvin G, Plavsic M. Gamma irradiation of animal sera for inactivation of viruses and mollicutes--a review. Biologicals. 2011;39:370-7 pubmed publisher
    ..Viral and mollicute (Mycoplasma sp. and Acholeplasma sp...
  49. Stakenborg T, Vicca J, Verhelst R, Butaye P, Maes D, Naessens A, et al. Evaluation of tRNA gene PCR for identification of mollicutes. J Clin Microbiol. 2005;43:4558-66 pubmed
    ..A total of 103 strains and DNA extracts of 30 different species belonging to the genera Acholeplasma, Mycoplasma, and Ureaplasma were studied...
  50. Platt M, Rottem S. Phosvitin kinase activity in Acholeplasma axanthum. FEBS Lett. 1988;242:97-100 pubmed
    ..0-6.2 and requires divalent cations. The activity was inhibited by ammonium sulfate, heparin and sulphydryl blocking reagents, but was not affected by calmodulin with or without Ca2+ or by cyclic AMP...
  51. Oradd G, Andersson A, Rilfors L, Lindblom G, Strandberg E, Andren P. alpha-methylene ordering of acyl chains differs in glucolipids and phosphatidylglycerol from Acholeplasma laidlawii membranes: (2)H-NMR quadrupole splittings from individual lipids in mixed bilayers. Biochim Biophys Acta. 2000;1468:329-44 pubmed
    A Acholeplasma laidlawii strain A-EF22 was grown in a medium supplemented with alpha-deuterated oleic acid...
  52. Otter J, Chewins J, Windsor D, Windsor H. Microbiological contamination in cell culture: a potential role for hydrogen peroxide vapour (HPV)?. Cell Biol Int. 2008;32:326-7 pubmed
  53. Sundaram S, Eisenhuth J, Steves M, Howard G, Brandwein H. Method for qualifying microbial removal performance of 0.1 micron rated filters. Part II: preliminary characterization of Hydrogenophaga (formerly Pseudomonas) pseudoflava for use as a standard challenge organism to qualify 0.1 micron rated filters. PDA J Pharm Sci Technol. 2001;55:373-92 pubmed
    ..1 microm rated filter types functionally qualified with a specified high titer reduction claim for Acholeplasma laidlawii, were shown to consistently and fully retain H...
  54. Mannock D, Lewis R, McElhaney R, Harper P, Turner D, Gruner S. An analysis of the relationship between fatty acid composition and the lamellar gel to liquid-crystalline and the lamellar to inverted nonlamellar phase transition temperatures of phosphatidylethanolamines and diacyl-alpha-D-glucosyl glycerols. Eur Biophys J. 2001;30:537-54 pubmed
  55. Wikström M, Xie J, Bogdanov M, Mileykovskaya E, Heacock P, Wieslander A, et al. Monoglucosyldiacylglycerol, a foreign lipid, can substitute for phosphatidylethanolamine in essential membrane-associated functions in Escherichia coli. J Biol Chem. 2004;279:10484-93 pubmed
    ..NB-prone neutral glycolipid alpha-monoglucosyldiacylglycerol (MGlcDAG) synthesized by the MGlcDAG synthase from Acholeplasma laidlawii. Dependence on Mg(2+) was reduced, and cellular yields and division malfunction were greatly improved...
  56. Kirchhoff H, Maass C, Runge M, Franz B, Schmidt R, Quentmeier H, et al. Tetrazolium [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction by mycoplasmas. Int J Syst Bacteriol. 1992;42:506-8 pubmed
    We investigated 22 mycoplasma and acholeplasma species for their ability to reduce tetrazolium salts by using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay...
  57. Tigges E, Minion F. Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping. J Bacteriol. 1994;176:1180-3 pubmed
    A physical chromosomal map of Acholeplasma oculi ISM1499 was constructed by using field inversion gel electrophoresis. To assist in the ordering of the chromosomal fragments, a modified transposon, Tn4001.1064, was constructed...
  58. Bascuñana C, Mattsson J, Bölske G, Johansson K. Characterization of the 16S rRNA genes from Mycoplasma sp. strain F38 and development of an identification system based on PCR. J Bacteriol. 1994;176:2577-86 pubmed
    ..Part of the 16S rRNA gene of the rrnA operon from strain F38 was also cloned, and several sequence differences between the two rRNA operons were discovered, revealing microheterogeneity between the two 16S rRNA genes of this organism...
  59. Neimark H, Lange C. Pulse-field electrophoresis indicates full-length Mycoplasma chromosomes range widely in size. Nucleic Acids Res. 1990;18:5443-8 pubmed
    ..Our PFGE estimates show that mycoplasma chromosomes span a continual range of sizes, with several intermediate values falling between the previously recognized large and small chromosome size clusters...
  60. Sjöström K, Kenny G. Differences in accumulation of radiolabeled amino acids and polyamines by Mycoplasma and Acholeplasma species. Int J Syst Bacteriol. 1990;40:456-61 pubmed
    Four species in the order Mycoplasmatales, Mycoplasma capricolum, Mycoplasma hominis, Mycoplasma arginini, and Acholeplasma laidlawii, were compared for their ability to accumulate radiolabeled amino acids and polyamines...
  61. Stipkovits L, Kobulej T, Varga Z, Juhasz S. In vitro testing of the anti-mycoplasma effect of some anti-coccidial drugs. Vet Microbiol. 1987;15:65-70 pubmed
    ..Forty-four strains representing 14 avian and 10 mammalian Mycoplasma species and serotypes and 5 Acholeplasma species were tested. The ionophores showed average minimal inhibitory concentration (MIC) values between 3...
  62. Namba S, Oyaizu H, Kato S, Iwanami S, Tsuchizaki T. Phylogenetic diversity of phytopathogenic mycoplasmalike organisms. Int J Syst Bacteriol. 1993;43:461-7 pubmed
    ..The three MLO groups are more closely related to Acholeplasma laidlawii than to Mycoplasma gallisepticum...
  63. Teyssou R, Poutiers F, Saillard C, Grau O, Laigret F, Bove J, et al. Detection of mollicute contamination in cell cultures by 16S rDNA amplification. Mol Cell Probes. 1993;7:209-16 pubmed
    ..two sets of primers able to promote amplification of all Mycoplasma and Ureaplasma (molli1-molli2a) or all Acholeplasma (molli1-molli2b) species examined were determined...
  64. Greenberg Ofrath N, Terespolosky Y, Kahane I, Bar R. Cyclodextrins as carriers of cholesterol and fatty acids in cultivation of mycoplasmas. Appl Environ Microbiol. 1993;59:547-51 pubmed
    ..dimethyl-beta-CD (Dimeb) and hydroxypropyl-beta-CD (Hyprob), on the growth of Mycoplasma capricolum and Acholeplasma laidlawii were investigated in a basal medium as well as in serum- and BSA-supplemented media...
  65. Li L, Karlsson O, Wieslander A. Activating amphiphiles cause a conformational change of the 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii membranes according to proteolytic digestion. J Biol Chem. 1997;272:29602-6 pubmed
    ..synthesizes the major nonbilayer-prone lipid monoglucosyldiacylglycerol (MGlcDAG) in the membrane of Acholeplasma laidlawii, which is important for the spontaneous curvature, and is a regulatory site for the lipid surface ..
  66. Wang H, Kong F, Jelfs P, James G, Gilbert G. Simultaneous detection and identification of common cell culture contaminant and pathogenic mollicutes strains by reverse line blot hybridization. Appl Environ Microbiol. 2004;70:1483-6 pubmed
    ..line contamination (Mycoplasma arginini, Mycoplasma fermentans, Mycoplasma hyorhinis, Mycoplasma orale, and Acholeplasma laidlawii) and human infection (Mycoplasma pneumoniae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma ..
  67. Staudegger E, Prenner E, Kriechbaum M, Degovics G, Lewis R, McElhaney R, et al. X-ray studies on the interaction of the antimicrobial peptide gramicidin S with microbial lipid extracts: evidence for cubic phase formation. Biochim Biophys Acta. 2000;1468:213-30 pubmed
    ..on the thermotropic phase behavior of model lipid bilayer membranes generated from the total membrane lipids of Acholeplasma laidlawii B and Escherichia coli. The A...
  68. Maniloff J. Mycoplasma viruses. Crit Rev Microbiol. 1988;15:339-89 pubmed
    ..This article is a critical review of mycoplasma viruses infecting the genera Acholeplasma, Spiroplasma, and Mycoplasma; included are data on classification, morphology and structure, biological and ..