rhodobacter capsulatus

Summary

Summary: Non-pathogenic ovoid to rod-shaped bacteria that are widely distributed and found in fresh water as well as marine and hypersaline habitats.

Top Publications

  1. Neumann M, Stöcklein W, Walburger A, Magalon A, Leimkuhler S. Identification of a Rhodobacter capsulatus L-cysteine desulfurase that sulfurates the molybdenum cofactor when bound to XdhC and before its insertion into xanthine dehydrogenase. Biochemistry. 2007;46:9586-95 pubmed
    ..XdhC was shown to be essential for the production of active XDH in Rhodobacter capsulatus but is itself not a subunit of the purified enzyme...
  2. Borsetti F, Toninello A, Zannoni D. Tellurite uptake by cells of the facultative phototroph Rhodobacter capsulatus is a Delta pH-dependent process. FEBS Lett. 2003;554:315-8 pubmed
    The uptake by light-grown cells of Rhodobacter capsulatus of the highly toxic metalloid oxyanion tellurite (TeO(3)(2-)) was examined...
  3. Nomata J, Kitashima M, Inoue K, Fujita Y. Nitrogenase Fe protein-like Fe-S cluster is conserved in L-protein (BchL) of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus. FEBS Lett. 2006;580:6151-4 pubmed
    ..Purified L-protein from Rhodobacter capsulatus showed absorption spectra and an electron paramagnetic resonance signal indicative of a [4Fe-4S] cluster...
  4. Pauff J, Hemann C, Jünemann N, Leimkuhler S, Hille R. The role of arginine 310 in catalysis and substrate specificity in xanthine dehydrogenase from Rhodobacter capsulatus. J Biol Chem. 2007;282:12785-90 pubmed
    The rapid reaction kinetics of wild-type xanthine dehydrogenase from Rhodobacter capsulatus and variants at Arg-310 in the active site have been characterized for a variety of purine substrates...
  5. Yakunin A, Hallenbeck P. AmtB is necessary for NH(4)(+)-induced nitrogenase switch-off and ADP-ribosylation in Rhodobacter capsulatus. J Bacteriol. 2002;184:4081-8 pubmed
    b>Rhodobacter capsulatus possesses two genes potentially coding for ammonia transporters, amtB and amtY...
  6. Fogg P, Hynes A, Digby E, Lang A, Beatty J. Characterization of a newly discovered Mu-like bacteriophage, RcapMu, in Rhodobacter capsulatus strain SB1003. Virology. 2011;421:211-21 pubmed publisher
    The ?-proteobacterium Rhodobacter capsulatus is a model organism for the study of bacterial photosynthesis and the bacteriophage-like gene transfer agent...
  7. Gisin J, Muller A, Pfänder Y, Leimkuhler S, Narberhaus F, Masepohl B. A Rhodobacter capsulatus member of a universal permease family imports molybdate and other oxyanions. J Bacteriol. 2010;192:5943-52 pubmed publisher
    ..To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis...
  8. Osyczka A, Moser C, Daldal F, Dutton P. Reversible redox energy coupling in electron transfer chains. Nature. 2004;427:607-12 pubmed
  9. Kessi J. Enzymic systems proposed to be involved in the dissimilatory reduction of selenite in the purple non-sulfur bacteria Rhodospirillum rubrum and Rhodobacter capsulatus. Microbiology. 2006;152:731-43 pubmed
    ..different enzymes in the reduction of selenite in the purple non-sulfur bacteria Rhodospirillum rubrum and Rhodobacter capsulatus. The hypothetical involvement of nitrite reductase and sulfite reductase in the reduction of selenite in ..

More Information

Publications87

  1. Lang A, Beatty J. Importance of widespread gene transfer agent genes in alpha-proteobacteria. Trends Microbiol. 2007;15:54-62 pubmed
    The gene transfer agent produced by Rhodobacter capsulatus (RcGTA) is a model for several virus-like elements that seem to function solely for mediating gene exchange...
  2. Ekici S, Jiang X, Koch H, Daldal F. Missense mutations in cytochrome c maturation genes provide new insights into Rhodobacter capsulatus cbb3-type cytochrome c oxidase biogenesis. J Bacteriol. 2013;195:261-9 pubmed publisher
    The Rhodobacter capsulatus cbb(3)-type cytochrome c oxidase (cbb(3)-Cox) belongs to the heme-copper oxidase superfamily, and its subunits are encoded by the ccoNOQP operon...
  3. Leimkuhler S, Hodson R, George G, Rajagopalan K. Recombinant Rhodobacter capsulatus xanthine dehydrogenase, a useful model system for the characterization of protein variants leading to xanthinuria I in humans. J Biol Chem. 2003;278:20802-11 pubmed
    b>Rhodobacter capsulatus xanthine dehydrogenase (XDH) forms an (alphabeta)2 heterotetramer and is highly homologous to homodimeric eukaryotic XDHs. The crystal structures of bovine XDH and R...
  4. Borsetti F, Tremaroli V, Michelacci F, Borghese R, Winterstein C, Daldal F, et al. Tellurite effects on Rhodobacter capsulatus cell viability and superoxide dismutase activity under oxidative stress conditions. Res Microbiol. 2005;156:807-13 pubmed
    Cells of the facultative photosynthetic bacterium Rhodobacter capsulatus (MT1131 strain) incubated with 10 microg ml-1 of the toxic oxyanion tellurite (TeO2-(3)) exhibited an increase in superoxide dismutase activity...
  5. Neumann M, Schulte M, Jünemann N, Stöcklein W, Leimkuhler S. Rhodobacter capsulatus XdhC is involved in molybdenum cofactor binding and insertion into xanthine dehydrogenase. J Biol Chem. 2006;281:15701-8 pubmed
    b>Rhodobacter capsulatus xanthine dehydrogenase (XDH) is a cytoplasmic enzyme with an (alphabeta)2 heterodimeric structure that is highly identical to homodimeric eukaryotic xanthine oxidoreductases...
  6. Czapla M, Borek A, Sarewicz M, Osyczka A. Fusing two cytochromes b of Rhodobacter capsulatus cytochrome bc1 using various linkers defines a set of protein templates for asymmetric mutagenesis. Protein Eng Des Sel. 2012;25:15-25 pubmed publisher
  7. Nomata J, Mizoguchi T, Tamiaki H, Fujita Y. A second nitrogenase-like enzyme for bacteriochlorophyll biosynthesis: reconstitution of chlorophyllide a reductase with purified X-protein (BchX) and YZ-protein (BchY-BchZ) from Rhodobacter capsulatus. J Biol Chem. 2006;281:15021-8 pubmed publisher
    ..Here, we report the reconstitution of chlorophyllide a reductase (COR) with purified proteins. Two Rhodobacter capsulatus strains that overexpressed Strep-tagged BchX and BchY were isolated...
  8. Chae P, Wander M, Bowling A, Laible P, Gellman S. Glycotripod amphiphiles for solubilization and stabilization of a membrane-protein superassembly: importance of branching in the hydrophilic portion. Chembiochem. 2008;9:1706-9 pubmed publisher
  9. Paoli G, Morgan N, Tabita F, Shively J. Expression of the cbbLcbbS and cbbM genes and distinct organization of the cbb Calvin cycle structural genes of Rhodobacter capsulatus. Arch Microbiol. 1995;164:396-405 pubmed
    b>Rhodobacter capsulatus fixes CO2 via the Calvin reductive pentose phosphate pathway and, like some other nonsulfur purple bacteria, is known to synthesize two distinct structural forms of ribulose 1,5-bisphosphate carboxylase/oxygenase (..
  10. Swierczek M, Cieluch E, Sarewicz M, Borek A, Moser C, Dutton P, et al. An electronic bus bar lies in the core of cytochrome bc1. Science. 2010;329:451-4 pubmed publisher
    ..Free and unregulated distribution of electrons acts like a molecular-scale bus bar, a design often exploited in electronics. ..
  11. Sarewicz M, Borek A, Cieluch E, Swierczek M, Osyczka A. Discrimination between two possible reaction sequences that create potential risk of generation of deleterious radicals by cytochrome bc?. Implications for the mechanism of superoxide production. Biochim Biophys Acta. 2010;1797:1820-7 pubmed publisher
    ..Isolation of this reaction sequence from multiplicity of possible reactions at Q(o) helps to better understand conditions under which complex III might contribute to ROS generation in vivo. ..
  12. Czapla M, Borek A, Sarewicz M, Osyczka A. Enzymatic activities of isolated cytochrome bc?-like complexes containing fused cytochrome b subunits with asymmetrically inactivated segments of electron transfer chains. Biochemistry. 2012;51:829-35 pubmed publisher
  13. Feniouk B, Cherepanov D, Voskoboynikova N, Mulkidjanian A, Junge W. Chromatophore vesicles of Rhodobacter capsulatus contain on average one F(O)F(1)-ATP synthase each. Biophys J. 2002;82:1115-22 pubmed
    ..Thereby chromatophores of Rhodobacter capsulatus provide a system where the coupling of proton transfer to ATP synthesis can be studied in a single enzyme/..
  14. Meister M, Saum S, Alber B, Fuchs G. L-malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium Rhodobacter capsulatus. J Bacteriol. 2005;187:1415-25 pubmed
    Cell extracts of Rhodobacter capsulatus grown on acetate contained an apparent malate synthase activity but lacked isocitrate lyase activity. Therefore, R...
  15. Leimkuhler S, Stockert A, Igarashi K, Nishino T, Hille R. The role of active site glutamate residues in catalysis of Rhodobacter capsulatus xanthine dehydrogenase. J Biol Chem. 2004;279:40437-44 pubmed
    Xanthine dehydrogenase (XDH) from the bacterium Rhodobacter capsulatus catalyzes the hydroxylation of xanthine to uric acid with NAD+ as the electron acceptor. R...
  16. Darrouzet E, Daldal F. Movement of the iron-sulfur subunit beyond the ef loop of cytochrome b is required for multiple turnovers of the bc1 complex but not for single turnover Qo site catalysis. J Biol Chem. 2002;277:3471-6 pubmed
  17. Cooley J, Ohnishi T, Daldal F. Binding dynamics at the quinone reduction (Qi) site influence the equilibrium interactions of the iron sulfur protein and hydroquinone oxidation (Qo) site of the cytochrome bc1 complex. Biochemistry. 2005;44:10520-32 pubmed
    ..Implications of these findings in respect to the Q(o)-Q(i) sites communications and to multiple turnovers of the cyt bc(1) are discussed...
  18. Neumann M, Stöcklein W, Leimkuhler S. Transfer of the molybdenum cofactor synthesized by Rhodobacter capsulatus MoeA to XdhC and MobA. J Biol Chem. 2007;282:28493-500 pubmed
    ..In bacteria such as Rhodobacter capsulatus and Escherichia coli, MPT is further modified by attachment of a GMP nucleotide, forming MPT guanine ..
  19. Poulain A, Newman D. Rhodobacter capsulatus catalyzes light-dependent Fe(II) oxidation under anaerobic conditions as a potential detoxification mechanism. Appl Environ Microbiol. 2009;75:6639-46 pubmed publisher
    ..study, we tested how light, Fe(II) speciation, pH, and salinity affected the rate of Fe(II) oxidation by Rhodobacter capsulatus SB1003. Although R...
  20. Eubanks L, Poulter C. Rhodobacter capsulatus 1-deoxy-D-xylulose 5-phosphate synthase: steady-state kinetics and substrate binding. Biochemistry. 2003;42:1140-9 pubmed
    ..These results are consistent with an ordered mechanism for DXP synthase where pyruvate binds before GAP/d-glyceraldehyde...
  21. Sluis M, Larsen R, Krum J, Anderson R, Metcalf W, Ensign S. Biochemical, molecular, and genetic analyses of the acetone carboxylases from Xanthobacter autotrophicus strain Py2 and Rhodobacter capsulatus strain B10. J Bacteriol. 2002;184:2969-77 pubmed
    ..In this study, the acetone carboxylase of the purple nonsulfur photosynthetic bacterium Rhodobacter capsulatus was purified to homogeneity and compared to that of Xanthobacter autotrophicus strain Py2, the only other ..
  22. Willett J, Smart J, Bauer C. RegA control of bacteriochlorophyll and carotenoid synthesis in Rhodobacter capsulatus. J Bacteriol. 2007;189:7765-73 pubmed
    ..vitro biochemical evidence that RegA directly regulates bacteriochlorophyll and carotenoid biosynthesis in Rhodobacter capsulatus. beta-Galactosidase expression assays with a RegA-disrupted strain containing reporter plasmids for Mg-..
  23. Zhang H, Osyczka A, Dutton P, Moser C. Exposing the complex III Qo semiquinone radical. Biochim Biophys Acta. 2007;1767:883-7 pubmed
  24. Drepper T, Wiethaus J, Giaourakis D, Gross S, Schubert B, Vogt M, et al. Cross-talk towards the response regulator NtrC controlling nitrogen metabolism in Rhodobacter capsulatus. FEMS Microbiol Lett. 2006;258:250-6 pubmed
    b>Rhodobacter capsulatus NtrB/NtrC two-component regulatory system controls expression of genes involved in nitrogen metabolism including urease and nitrogen fixation genes...
  25. Swem D, Bauer C. Coordination of ubiquinol oxidase and cytochrome cbb(3) oxidase expression by multiple regulators in Rhodobacter capsulatus. J Bacteriol. 2002;184:2815-20 pubmed
    b>Rhodobacter capsulatus utilizes two terminal oxidases for aerobic respiration, cytochrome cbb(3) and ubiquinol oxidase...
  26. Sicking C, Brusch M, Lindackers A, Riedel K, Schubert B, Isakovic N, et al. Identification of two new genes involved in diazotrophic growth via the alternative Fe-only nitrogenase in the phototrophic purple bacterium Rhodobacter capsulatus. J Bacteriol. 2005;187:92-8 pubmed
    Growth of Rhodobacter capsulatus with molecular dinitrogen as the sole N source via the alternative Fe-only nitrogenase requires all seven gene products of the anfHDGK-1-2-3 operon...
  27. Bittel C, Tabares L, Armesto M, Carrillo N, Cortez N. The oxidant-responsive diaphorase of Rhodobacter capsulatus is a ferredoxin (flavodoxin)-NADP(H) reductase. FEBS Lett. 2003;553:408-12 pubmed
    Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide ..
  28. Ekici S, Pawlik G, Lohmeyer E, Koch H, Daldal F. Biogenesis of cbb(3)-type cytochrome c oxidase in Rhodobacter capsulatus. Biochim Biophys Acta. 2012;1817:898-910 pubmed publisher
    ..This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes...
  29. Swem L, Elsen S, Bird T, Swem D, Koch H, Myllykallio H, et al. The RegB/RegA two-component regulatory system controls synthesis of photosynthesis and respiratory electron transfer components in Rhodobacter capsulatus. J Mol Biol. 2001;309:121-38 pubmed
    Recently, we demonstrated that the RegB/RegA two-component regulatory system from Rhodobacter capsulatus functions as a global regulator of metabolic processes that either generate or consume reducing equivalents...
  30. Drepper T, Gross S, Yakunin A, Hallenbeck P, Masepohl B, Klipp W. Role of GlnB and GlnK in ammonium control of both nitrogenase systems in the phototrophic bacterium Rhodobacter capsulatus. Microbiology. 2003;149:2203-12 pubmed
    ..b>Rhodobacter capsulatus possesses two genes (glnB and glnK) encoding P(II)-like proteins...
  31. Conrad C, Rauhut R, Klug G. Different cleavage specificities of RNases III from Rhodobacter capsulatus and Escherichia coli. Nucleic Acids Res. 1998;26:4446-53 pubmed
    23S rRNA in Rhodobacter capsulatus shows endoribonuclease III (RNase III)-dependent fragmentation in vivo at a unique extra stem-loop extending from position 1271 to 1331. RNase III is a double strand (ds)-specific endoribonuclease...
  32. Przybylski M, Glocker M, Nestel U, Schnaible V, Blüggel M, Diederichs K, et al. X-ray crystallographic and mass spectrometric structure determination and functional characterization of succinylated porin from Rhodobacter capsulatus: implications for ion selectivity and single-channel conductance. Protein Sci. 1996;5:1477-89 pubmed
    ..To introduce new negative charges in a channel protein, amino groups of porin from Rhodobacter capsulatus 37b4 were succinylated with succinic anhydride, and the precise extent and sites of succinylations and ..
  33. Gough S, Petersen B, Duus J. Anaerobic chlorophyll isocyclic ring formation in Rhodobacter capsulatus requires a cobalamin cofactor. Proc Natl Acad Sci U S A. 2000;97:6908-13 pubmed
    ..We found that vitamin B(12) (B(12))-requiring mutants of the bluE and bluB genes of Rhodobacter capsulatus, grown without B(12), accumulated Mg-porphyrins...
  34. Yamamoto H, Nomata J, Fuita Y. Functional expression of nitrogenase-like protochlorophyllide reductase from Rhodobacter capsulatus in Escherichia coli. Photochem Photobiol Sci. 2008;7:1238-42 pubmed publisher
    ..Here we report the functional expression of DPOR components from Rhodobacter capsulatus in Escherichia coli. Two overexpression plasmids for L-protein and NB-protein were constructed...
  35. Shelswell K, Beatty J. Coordinated, long-range, solid substrate movement of the purple photosynthetic bacterium Rhodobacter capsulatus. PLoS ONE. 2011;6:e19646 pubmed publisher
    The long-range movement of Rhodobacter capsulatus cells in the glass-agar interstitial region of borosilicate Petri plates was found to be due to a subset of the cells inoculated into plates...
  36. Lanciano P, Lee D, Yang H, Darrouzet E, Daldal F. Intermonomer electron transfer between the low-potential b hemes of cytochrome bc?. Biochemistry. 2011;50:1651-63 pubmed publisher
    ..intra- and intermonomer interactions within the cyt bc(1) using the facultative phototrophic bacterium Rhodobacter capsulatus. The system involves two different sets of independently expressed cyt bc(1) structural genes carried by ..
  37. Sarewicz M, Dutka M, Froncisz W, Osyczka A. Magnetic interactions sense changes in distance between heme b(L) and the iron-sulfur cluster in cytochrome bc(1). Biochemistry. 2009;48:5708-20 pubmed publisher
    ..The measured changes in the phase relaxation enhancement provide the first direct experimental description of changes in the strength of dipolar coupling between the FeS cluster and heme b(L). ..
  38. Fogg P, Westbye A, Beatty J. One for all or all for one: heterogeneous expression and host cell lysis are key to gene transfer agent activity in Rhodobacter capsulatus. PLoS ONE. 2012;7:e43772 pubmed publisher
    The gene transfer agent (RcGTA) of Rhodobacter capsulatus is the model for a family of novel bacteriophage-related genetic elements that carry out lateral transfer of essentially random host DNA...
  39. Masuda S, Bauer C. Null mutation of HvrA compensates for loss of an essential relA/spoT-like gene in Rhodobacter capsulatus. J Bacteriol. 2004;186:235-9 pubmed
    We report that a single relA/spoT-like gene exists on the Rhodobacter capsulatus chromosome, and its mutational loss is lethal. This gene could be mutated only under a mutational background of a null mutation in the nucleoid protein HvrA...
  40. Borghese R, Cicerano S, Zannoni D. Fructose increases the resistance of Rhodobacter capsulatus to the toxic oxyanion tellurite through repression of acetate permease (ActP). Antonie Van Leeuwenhoek. 2011;100:655-8 pubmed publisher
    ..2-)) enters the cells of the facultative photosynthetic bacterium Rhodobacter capsulatus through an acetate permease...
  41. Bollivar D, Clauson C, Lighthall R, Forbes S, Kokona B, Fairman R, et al. Rhodobacter capsulatus porphobilinogen synthase, a high activity metal ion independent hexamer. BMC Biochem. 2004;5:17 pubmed
    ..This paper reports new information on the metal ion independence and quaternary structure of PBGS from the photosynthetic bacterium Rhodobacter capsulatus.
  42. Forward J, Behrendt M, Wyborn N, Cross R, Kelly D. TRAP transporters: a new family of periplasmic solute transport systems encoded by the dctPQM genes of Rhodobacter capsulatus and by homologs in diverse gram-negative bacteria. J Bacteriol. 1997;179:5482-93 pubmed
    The dct locus of Rhodobacter capsulatus encodes a high-affinity transport system for the C4-dicarboxylates malate, succinate, and fumarate...
  43. Deshmukh M, Turkarslan S, Astor D, Valkova Valchanova M, Daldal F. The dithiol:disulfide oxidoreductases DsbA and DsbB of Rhodobacter capsulatus are not directly involved in cytochrome c biogenesis, but their inactivation restores the cytochrome c biogenesis defect of CcdA-null mutants. J Bacteriol. 2003;185:3361-72 pubmed
    ..CcdA-null mutants of the facultative phototroph Rhodobacter capsulatus are unable to grow under photosynthetic conditions (Ps(-)) and do not produce any active cytochrome c ..
  44. Vignais P, Dimon B, Zorin N, Tomiyama M, Colbeau A. Characterization of the hydrogen-deuterium exchange activities of the energy-transducing HupSL hydrogenase and H(2)-signaling HupUV hydrogenase in Rhodobacter capsulatus. J Bacteriol. 2000;182:5997-6004 pubmed
    b>Rhodobacter capsulatus synthesizes two homologous protein complexes capable of activating molecular H(2), a membrane-bound [NiFe] hydrogenase (HupSL) linked to the respiratory chain, and an H(2) sensor encoded by the hupUV genes...
  45. Bortolotti A, Perez Dorado I, Goñi G, Medina M, Hermoso J, Carrillo N, et al. Coenzyme binding and hydride transfer in Rhodobacter capsulatus ferredoxin/flavodoxin NADP(H) oxidoreductase. Biochim Biophys Acta. 2009;1794:199-210 pubmed publisher
    ..We investigated nucleotide interactions and hydride transfer in Rhodobacter capsulatus FPR comparing them to those reported for FNRs...
  46. Lee D, Ozturk Y, Osyczka A, Cooley J, Daldal F. Cytochrome bc1-cy fusion complexes reveal the distance constraints for functional electron transfer between photosynthesis components. J Biol Chem. 2008;283:13973-82 pubmed publisher
    Photosynthetic (Ps) growth of purple non-sulfur bacteria such as Rhodobacter capsulatus depends on the cyclic electron transfer (ET) between the ubihydroquinone (QH2): cytochrome (cyt) c oxidoreductases (cyt bc1 complex), and the ..
  47. Kumagai H, Fujiwara T, Matsubara H, Saeki K. Membrane localization, topology, and mutual stabilization of the rnfABC gene products in Rhodobacter capsulatus and implications for a new family of energy-coupling NADH oxidoreductases. Biochemistry. 1997;36:5509-21 pubmed publisher
    The rnf genes in Rhodobacter capsulatus are unique nitrogen fixation genes that encode potential membrane proteins (RnfA, RnfD, and RnfE) and potential iron-sulfur proteins (RnfB and RnfC)...
  48. Mulkidjanian A. Proton translocation by the cytochrome bc1 complexes of phototrophic bacteria: introducing the activated Q-cycle. Photochem Photobiol Sci. 2007;6:19-34 pubmed
    ..Then the oxidation of each ubiquinol molecule in center P is followed by ubiquinol formation in center N, proton translocation and generation of membrane voltage...
  49. Adams B, Smith A, Bailey S, McEwan A, Bray R. Reactions of dimethylsulfoxide reductase from Rhodobacter capsulatus with dimethyl sulfide and with dimethyl sulfoxide: complexities revealed by conventional and stopped-flow spectrophotometry. Biochemistry. 1999;38:8501-11 pubmed
    ..The implications of this new species are discussed in relation both to conflicting published information for DMSOR from X-ray crystallography and to previous spectroscopic data for its reduced forms...
  50. Miyashita O, Onuchic J, Okamura M. Transition state and encounter complex for fast association of cytochrome c2 with bacterial reaction center. Proc Natl Acad Sci U S A. 2004;101:16174-9 pubmed
    ..The similarity between the structures of the EC, TS, and bound state can account for the rapid rate of association responsible for fast diffusion-controlled electron transfer...
  51. Cooley J, Roberts A, Bowman M, Kramer D, Daldal F. The raised midpoint potential of the [2Fe2S] cluster of cytochrome bc1 is mediated by both the Qo site occupants and the head domain position of the Fe-S protein subunit. Biochemistry. 2004;43:2217-27 pubmed
    We have previously reported that mutant strains of Rhodobacter capsulatus that have alanine insertions (+nAla mutants) in the hinge region of the iron sulfur (Fe-S) containing subunit of the bc(1) complex have increased redox midpoint ..
  52. Borghese R, Zannoni D. Acetate permease (ActP) Is responsible for tellurite (TeO32-) uptake and resistance in cells of the facultative phototroph Rhodobacter capsulatus. Appl Environ Microbiol. 2010;76:942-4 pubmed publisher
    ..Here we show that in the phototroph Rhodobacter capsulatus, tellurite exploits acetate permease (ActP) to get into the cytoplasm and that the levels of resistance ..
  53. Nomata J, Kondo T, Itoh S, Fujita Y. Nicotinamide is a specific inhibitor of dark-operative protochlorophyllide oxidoreductase, a nitrogenase-like enzyme, from Rhodobacter capsulatus. FEBS Lett. 2013;587:3142-7 pubmed publisher
    ..A reaction scheme of DPOR, in which the binding of protochlorophyllide (Pchlide) to the NB-protein precedes the electron transfer from the L-protein, is proposed based on the NA effects. ..
  54. Muraki N, Nomata J, Ebata K, Mizoguchi T, Shiba T, Tamiaki H, et al. X-ray crystal structure of the light-independent protochlorophyllide reductase. Nature. 2010;465:110-4 pubmed publisher
    ..Here we report the crystal structure of the NB-protein of DPOR from Rhodobacter capsulatus at a resolution of 2.3A...
  55. Lohmeyer E, Schröder S, Pawlik G, Trasnea P, Peters A, Daldal F, et al. The ScoI homologue SenC is a copper binding protein that interacts directly with the cbb?-type cytochrome oxidase in Rhodobacter capsulatus. Biochim Biophys Acta. 2012;1817:2005-15 pubmed publisher
    ..However, Sco homologues are also found in bacteria like Rhodobacter capsulatus which lack aa?-type cytochrome oxidases and instead use a cbb?-type cytochrome oxidase (cbb? Cox) without ..
  56. Westbye A, Leung M, Florizone S, Taylor T, Johnson J, Fogg P, et al. Phosphate concentration and the putative sensor kinase protein CckA modulate cell lysis and release of the Rhodobacter capsulatus gene transfer agent. J Bacteriol. 2013;195:5025-40 pubmed publisher
    The gene transfer agent of Rhodobacter capsulatus (RcGTA) is a bacteriophage-like genetic element with the sole known function of horizontal gene transfer...
  57. Osyczka A, Dutton P, Moser C, Darrouzet E, Daldal F. Controlling the functionality of cytochrome c(1) redox potentials in the Rhodobacter capsulatus bc(1) complex through disulfide anchoring of a loop and a beta-branched amino acid near the heme-ligating methionine. Biochemistry. 2001;40:14547-56 pubmed
    ..The disulfide anchored loop and betaXM motifs appear to be two independent but nonadditive strategies to control the integrity of the heme-binding pocket and raise cytochrome c midpoint potentials...
  58. Richard C, Tandon A, Kranz R. Rhodobacter capsulatus nifA1 promoter: high-GC -10 regions in high-GC bacteria and the basis for their transcription. J Bacteriol. 2004;186:740-9 pubmed
    It was previously shown that the Rhodobacter capsulatus NtrC enhancer-binding protein activates the R. capsulatus housekeeping RNA polymerase but not the Escherichia coli RNA polymerase at the nifA1 promoter...
  59. McGoldrick H, Roessner C, Raux E, Lawrence A, McLean K, Munro A, et al. Identification and characterization of a novel vitamin B12 (cobalamin) biosynthetic enzyme (CobZ) from Rhodobacter capsulatus, containing flavin, heme, and Fe-S cofactors. J Biol Chem. 2005;280:1086-94 pubmed
    ..However, in the photosynthetic bacterium Rhodobacter capsulatus, which harbors an aerobic-like pathway, there is no cobG in the main cobalamin biosynthetic operon ..
  60. Kyndt J, Hurley J, Devreese B, Meyer T, Cusanovich M, Tollin G, et al. Rhodobacter capsulatus photoactive yellow protein: genetic context, spectral and kinetics characterization, and mutagenesis. Biochemistry. 2004;43:1809-20 pubmed
    A gene for photoactive yellow protein (PYP) was previously cloned from Rhodobacter capsulatus (Rc), and we have now found it to be associated with genes for gas vesicle formation in the recently completed genome sequence...
  61. Willison J, Tissot G. The Escherichia coli efg gene and the Rhodobacter capsulatus adgA gene code for NH3-dependent NAD synthetase. J Bacteriol. 1994;176:3400-2 pubmed
    The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase...
  62. Lang A, Taylor T, Beatty J. Evolutionary implications of phylogenetic analyses of the gene transfer agent (GTA) of Rhodobacter capsulatus. J Mol Evol. 2002;55:534-43 pubmed
    The gene transfer agent (GTA) of the a-proteobacterium Rhodobacter capsulatus is a cell-controlled genetic exchange vector. Genes that encode the GTA structure are clustered in a 15-kb region of the R...
  63. Schaefer A, Taylor T, Beatty J, Greenberg E. Long-chain acyl-homoserine lactone quorum-sensing regulation of Rhodobacter capsulatus gene transfer agent production. J Bacteriol. 2002;184:6515-21 pubmed
    ..that did not require a bioassay to detect production of long-acyl-chain homoserine lactone production by Rhodobacter capsulatus and Paracoccus denitrificans...
  64. Kilic M, Spiro S, Moore G. Stability of a 24-meric homopolymer: comparative studies of assembly-defective mutants of Rhodobacter capsulatus bacterioferritin and the native protein. Protein Sci. 2003;12:1663-74 pubmed
    The stability of Rhodobacter capsulatus bacterioferritin, a 24-meric homopolymer, toward denaturation on variation in pH and temperature, and increasing concentrations of urea and guanidine...
  65. Borghese R, Borsetti F, Foladori P, Ziglio G, Zannoni D. Effects of the metalloid oxyanion tellurite (TeO32-) on growth characteristics of the phototrophic bacterium Rhodobacter capsulatus. Appl Environ Microbiol. 2004;70:6595-602 pubmed
    ..examines the effects of potassium tellurite (K2TeO3) on the cell viability of the facultative phototroph Rhodobacter capsulatus. There was a growth mode-dependent response in which cultures anaerobically grown in the light tolerate ..
  66. Nomata J, Swem L, Bauer C, Fujita Y. Overexpression and characterization of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus. Biochim Biophys Acta. 2005;1708:229-37 pubmed
    ..components of DPOR, L-protein (BchL) and NB-protein (BchN-BchB), in the broad-host-range vector pJRD215 in Rhodobacter capsulatus. We established a stable DPOR assay system by mixing crude extracts from the two transconjugants under ..
  67. Li K, Hein S, Zou W, Klug G. The glutathione-glutaredoxin system in Rhodobacter capsulatus: part of a complex regulatory network controlling defense against oxidative stress. J Bacteriol. 2004;186:6800-8 pubmed
    Mutants with defects in components of the glutathione-glutaredoxin (GSH/Grx) system of Rhodobacter capsulatus were constructed to study its role in defense against oxidative stress and the redox-dependent formation of photosynthetic ..
  68. Tremblay P, Drepper T, Masepohl B, Hallenbeck P. Membrane sequestration of PII proteins and nitrogenase regulation in the photosynthetic bacterium Rhodobacter capsulatus. J Bacteriol. 2007;189:5850-9 pubmed
    Both Rhodobacter capsulatus PII homologs GlnB and GlnK were found to be necessary for the proper regulation of nitrogenase activity and modification in response to an ammonium shock...
  69. Mulkidjanian A. Activated Q-cycle as a common mechanism for cytochrome bc1 and cytochrome b6f complexes. Biochim Biophys Acta. 2010;1797:1858-68 pubmed publisher
    ..redox changes of cytochromes b and c(1), voltage generation, and proton transfer in membrane vesicles of Rhodobacter capsulatus, we have put forward a scheme of an "activated Q-cycle" for the bc(1)...
  70. Shelswell K, Taylor T, Beatty J. Photoresponsive flagellum-independent motility of the purple phototrophic bacterium Rhodobacter capsulatus. J Bacteriol. 2005;187:5040-3 pubmed
    We report the discovery of photoresponsive, flagellum-independent motility of the alpha-proteobacterium Rhodobacter capsulatus, a nonsulfur purple phototrophic bacterium. This motility takes place in the 1...
  71. Li J, Osyczka A, Conover R, Johnson M, Qin H, Daldal F, et al. Role of acidic and aromatic amino acids in Rhodobacter capsulatus cytochrome c1. A site-directed mutagenesis study. Biochemistry. 2003;42:8818-30 pubmed
    The roles of two evolutionarily conserved aromatic residues in the cytochrome c(1) component of the Rhodobacter capsulatus cytochrome bc(1) complex, phenylalanine 138 and tyrosine 194, were analyzed by site-directed mutagenesis, in ..
  72. Leung M, Brimacombe C, Spiegelman G, Beatty J. The GtaR protein negatively regulates transcription of the gtaRI operon and modulates gene transfer agent (RcGTA) expression in Rhodobacter capsulatus. Mol Microbiol. 2012;83:759-74 pubmed publisher
    The gtaI gene of Rhodobacter capsulatus encodes an N-acyl-homoserine lactone (acyl-HSL) synthase. Immediately 5' of the gtaI gene is ORF rcc00328 that encodes a potential acyl-HSL receptor protein...
  73. Leung M, Florizone S, Taylor T, Lang A, Beatty J. The gene transfer agent of Rhodobacter capsulatus. Adv Exp Med Biol. 2010;675:253-64 pubmed publisher
    When Rhodobacter capsulatus cultures enter the stationary phase of growth, particles of the gene transfer agent (RcGTA) are released from cells...
  74. Lee D, Ozturk Y, Mamedova A, Osyczka A, Cooley J, Daldal F. A functional hybrid between the cytochrome bc1 complex and its physiological membrane-anchored electron acceptor cytochrome cy in Rhodobacter capsulatus. Biochim Biophys Acta. 2006;1757:346-52 pubmed
    ..In Rhodobacter capsulatus, it has been observed previously that, depending on the growth condition, absence of the cyt bc1 complex ..
  75. Mercer R, Callister S, Lipton M, Pasa Tolic L, Strnad H, Paces V, et al. Loss of the response regulator CtrA causes pleiotropic effects on gene expression but does not affect growth phase regulation in Rhodobacter capsulatus. J Bacteriol. 2010;192:2701-10 pubmed publisher
    The purple nonsulfur photosynthetic bacterium Rhodobacter capsulatus has been extensively studied for its metabolic versatility as well as for production of a gene transfer agent called RcGTA...
  76. Zappa S, Li K, Bauer C. The tetrapyrrole biosynthetic pathway and its regulation in Rhodobacter capsulatus. Adv Exp Med Biol. 2010;675:229-50 pubmed publisher
    The purple anoxygenic photosynthetic bacterium Rhodobacter capsulatus is capable of growing in aerobic or anaerobic conditions, in the dark or using light, etc...
  77. Lang A, Beatty J. A bacterial signal transduction system controls genetic exchange and motility. J Bacteriol. 2002;184:913-8 pubmed
    The bacterium Rhodobacter capsulatus is capable of an unusual form of genetic exchange, mediated by a transducing bacteriophage-like particle called the gene transfer agent (GTA). GTA production by R...
  78. Kranz R, Gabbert K, Locke T, Madigan M. Polyhydroxyalkanoate production in Rhodobacter capsulatus: genes, mutants, expression, and physiology. Appl Environ Microbiol. 1997;63:3003-9 pubmed
    Like many other prokaryotes, the photosynthetic bacterium Rhodobacter capsulatus produces high levels of polyhydroxyalkanoates (PHAs) when a suitable carbon source is available...