lactococcus lactis

Summary

Summary: A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.

Top Publications

  1. Pontes D, Innocentin S, Del Carmen S, Almeida J, LeBlanc J, de Moreno de Leblanc A, et al. Production of Fibronectin Binding Protein A at the surface of Lactococcus lactis increases plasmid transfer in vitro and in vivo. PLoS ONE. 2012;7:e44892 pubmed publisher
    ..We previously showed that Lactococcus lactis (LL) expressing the Fibronectin-Binding Protein A of Staphylococcus aureus (LL-FnBPA+) showed higher ..
  2. Racle J, Picard F, Girbal L, Cocaign Bousquet M, Hatzimanikatis V. A genome-scale integration and analysis of Lactococcus lactis translation data. PLoS Comput Biol. 2013;9:e1003240 pubmed publisher
    ..We established a procedure for robust estimation of the ribosomal density in hundreds of genes from Lactococcus lactis polysome size measurements...
  3. Achilleos C, Berthier F. Quantitative PCR for the specific quantification of Lactococcus lactis and Lactobacillus paracasei and its interest for Lactococcus lactis in cheese samples. Food Microbiol. 2013;36:286-95 pubmed publisher
    ..of mesophilic lactic acid bacteria technologically active in food fermentation, including cheese making: Lactococcus lactis and Lactobacillus paracasei...
  4. Eckhardt T, Skotnicka D, Kok J, Kuipers O. Transcriptional regulation of fatty acid biosynthesis in Lactococcus lactis. J Bacteriol. 2013;195:1081-9 pubmed publisher
    ..fatty acid biosynthesis (FAB) regulator FabT (originally called RmaG [Llmg_1788]) on gene transcription in Lactococcus lactis MG1363...
  5. Habimana O, Guillier L, Kulakauskas S, Briandet R. Spatial competition with Lactococcus lactis in mixed-species continuous-flow biofilms inhibits Listeria monocytogenes growth. Biofouling. 2011;27:1065-72 pubmed publisher
    ..monocytogenes cells were inhibited by the presence of a model resident flora (Lactococcus lactis) in dual-species continuous flow-biofilms, and are spatially restricted to the lower biofilm layers...
  6. Picard F, Milhem H, Loubiere P, Laurent B, Cocaign Bousquet M, Girbal L. Bacterial translational regulations: high diversity between all mRNAs and major role in gene expression. BMC Genomics. 2012;13:528 pubmed publisher
    ..To experimentally explore mRNA translational level regulation at the systemic level, the detailed translational status (translatome) of all mRNAs was measured in the model bacterium Lactococcus lactis in exponential phase growth.
  7. Jing H, Yong L, Haiyan L, Yanjun M, Yun X, Yu Z, et al. Oral administration of Lactococcus lactis delivered heat shock protein 65 attenuates atherosclerosis in low-density lipoprotein receptor-deficient mice. Vaccine. 2011;29:4102-9 pubmed publisher
    ..to investigate the effect of oral administration of mycobacterial heat shock protein 65 (HSP65) delivered by Lactococcus lactis (L. lactis) on atherogenesis...
  8. Guo T, Hu S, Kong J. Functional analysis and randomization of the nisin-inducible promoter for tuning gene expression in Lactococcus lactis. Curr Microbiol. 2013;66:548-54 pubmed publisher
    Three putative promoter regions were identified preceding the nisZ gene in Lactococcus lactis HSM-22...
  9. Fischer K, Stein K, Ulmer A, Lindner B, Heine H, Holst O. Cytokine-inducing lipoteichoic acids of the allergy-protective bacterium Lactococcus lactis G121 do not activate via Toll-like receptor 2. Glycobiology. 2011;21:1588-95 pubmed publisher
    It was established in a mouse model that the cowshed Gram-positive bacterium Lactococcus lactis G121 modulates the immune system resulting in allergy protection...

More Information

Publications99

  1. Daniel C, Poiret S, Dennin V, Boutillier D, Pot B. Bioluminescence imaging study of spatial and temporal persistence of Lactobacillus plantarum and Lactococcus lactis in living mice. Appl Environ Microbiol. 2013;79:1086-94 pubmed publisher
    ..this study, we describe the development and application of luciferase-expressing Lactobacillus plantarum and Lactococcus lactis strains for noninvasive in vivo monitoring in the digestive tract of mice...
  2. Oh E, Lu M, Park C, Oh H, Lee S, Lee J. Dynamic modeling of lactic acid fermentation metabolism with Lactococcus lactis. J Microbiol Biotechnol. 2011;21:162-9 pubmed
    A dynamic model of lactic acid fermentation using Lactococcus lactis was constructed, and a metabolic flux analysis (MFA) and metabolic control analysis (MCA) were performed to reveal an intensive metabolic understanding of lactic acid ..
  3. Asmat T, Klingbeil K, Jensch I, Burchhardt G, Hammerschmidt S. Heterologous expression of pneumococcal virulence factor PspC on the surface of Lactococcus lactis confers adhesive properties. Microbiology. 2012;158:771-80 pubmed publisher
    b>Lactococcus lactis is a non-pathogenic bacterium that is used in the food industry but is also used as a heterologous host to reveal protein functions of pathogenic bacteria...
  4. Nicolaou N, Xu Y, Goodacre R. Fourier transform infrared and Raman spectroscopies for the rapid detection, enumeration, and growth interaction of the bacteria Staphylococcus aureus and Lactococcus lactis ssp. cremoris in milk. Anal Chem. 2011;83:5681-7 pubmed publisher
    ..aureus in milk, as well as to study the growth interaction between S. aureus and Lactococcus lactis ssp. cremoris , a common LAB...
  5. Oxaran V, Ledue Clier F, Dieye Y, Herry J, Pechoux C, Meylheuc T, et al. Pilus biogenesis in Lactococcus lactis: molecular characterization and role in aggregation and biofilm formation. PLoS ONE. 2012;7:e50989 pubmed publisher
    The genome of Lactococcus lactis strain IL1403 harbors a putative pilus biogenesis cluster consisting of a sortase C gene flanked by 3 LPxTG protein encoding genes (yhgD, yhgE, and yhhB), called here pil...
  6. van Pijkeren J, Britton R. High efficiency recombineering in lactic acid bacteria. Nucleic Acids Res. 2012;40:e76 pubmed publisher
    ..Mutations were incorporated in the chromosome of Lactobacillus reuteri and Lactococcus lactis without selection at frequencies ranging between 0.4% and 19%...
  7. Maddocks S, Wright C, Nobbs A, Brittan J, Franklin L, Strömberg N, et al. Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation. Mol Microbiol. 2011;81:1034-49 pubmed publisher
    ..In this study it is shown that AspA from serotype M28 S. pyogenes, when expressed on surrogate host Lactococcus lactis, confers binding to immobilized salivary agglutinin gp-340...
  8. Shinkawa S, Okano K, Yoshida S, Tanaka T, Ogino C, Fukuda H, et al. Improved homo L-lactic acid fermentation from xylose by abolishment of the phosphoketolase pathway and enhancement of the pentose phosphate pathway in genetically modified xylose-assimilating Lactococcus lactis. Appl Microbiol Biotechnol. 2011;91:1537-44 pubmed publisher
    ..homo L-lactic acid fermentation from xylose, we first carried out addition of xylose assimilation ability to Lactococcus lactis IL 1403 by introducing a plasmid carrying the xylRAB genes from L. lactis IO-1 (pXylRAB)...
  9. Ladero V, Rattray F, Mayo B, Martín M, Fernandez M, Alvarez M. Sequencing and transcriptional analysis of the biosynthesis gene cluster of putrescine-producing Lactococcus lactis. Appl Environ Microbiol. 2011;77:6409-18 pubmed publisher
    b>Lactococcus lactis is a prokaryotic microorganism with great importance as a culture starter and has become the model species among the lactic acid bacteria. The long and safe history of use of L...
  10. Górecki R, Koryszewska Bagińska A, Gołebiewski M, Zylińska J, Grynberg M, Bardowski J. Adaptative potential of the Lactococcus lactis IL594 strain encoded in its 7 plasmids. PLoS ONE. 2011;6:e22238 pubmed publisher
    ..g. genes encoding citrate and lactose utilization, oligopeptide transport, restriction-modification system). Moreover, global gene analysis indicated cooperation between plasmid- and chromosome-encoded metabolic pathways. ..
  11. Kato H, Shiwa Y, Oshima K, Machii M, Araya Kojima T, Zendo T, et al. Complete genome sequence of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid. J Bacteriol. 2012;194:2102-3 pubmed publisher
    We report the complete genome sequence of Lactococcus lactis IO-1 (= JCM7638). It is a nondairy lactic acid bacterium, produces nisin Z, ferments xylose, and produces predominantly L-lactic acid at high xylose concentrations...
  12. Bolotin A, Quinquis B, Ehrlich S, Sorokin A. Complete genome sequence of Lactococcus lactis subsp. cremoris A76. J Bacteriol. 2012;194:1241-2 pubmed publisher
    We report the complete genome sequence of Lactococcus lactis subsp. cremoris A76, a dairy strain isolated from a cheese production outfit. Genome analysis detected two contiguous islands fitting to the L. lactis subsp...
  13. Le D, Gu rardel Y, Loubi re P, Mercier Bonin M, Dague E. Measuring kinetic dissociation/association constants between Lactococcus lactis bacteria and mucins using living cell probes. Biophys J. 2011;101:2843-53 pubmed publisher
    In this work we focused on quantifying adhesion between Lactococcus lactis, the model for lactic acid bacteria (LAB) and mucins. Interactions between two strains of L...
  14. Kosaka A, Yan H, Ohashi S, Gotoh Y, Sato A, Tsutsui H, et al. Lactococcus lactis subsp. cremoris FC triggers IFN-? production from NK and T cells via IL-12 and IL-18. Int Immunopharmacol. 2012;14:729-33 pubmed publisher
    ..In this study, we investigated the immunomodulatory effects of Lactococcus lactis subsp. cremoris FC (LcFC) on dendritic cells (DCs), natural killer (NK) cells and T cells...
  15. Garg N, Salazar Ocampo L, van der Donk W. In vitro activity of the nisin dehydratase NisB. Proc Natl Acad Sci U S A. 2013;110:7258-63 pubmed publisher
    ..The latter step has precedent in the virginiamycin resistance protein virginiamycin B lyase. These studies will facilitate investigation of other LanB proteins involved in the biosynthesis of lantibiotics, thiopeptides, and goadsporin. ..
  16. Odamaki T, Xiao J, Yonezawa S, Yaeshima T, Iwatsuki K. Improved viability of bifidobacteria in fermented milk by cocultivation with Lactococcus lactis subspecies lactis. J Dairy Sci. 2011;94:1112-21 pubmed publisher
    ..Cocultivation with some strains of Lactococcus lactis ssp. lactis improved the survival of bifidobacteria in fermented milk during refrigerated storage...
  17. Hugentobler F, Di Roberto R, Gillard J, Cousineau B. Oral immunization using live Lactococcus lactis co-expressing LACK and IL-12 protects BALB/c mice against Leishmania major infection. Vaccine. 2012;30:5726-32 pubmed publisher
    ..b>Lactococcus lactis is a non-pathogenic, non-colonizing Gram-positive lactic acid bacterium commonly used in the dairy industry...
  18. Carvalho A, Cardoso F, Bohn A, Neves A, Santos H. Engineering trehalose synthesis in Lactococcus lactis for improved stress tolerance. Appl Environ Microbiol. 2011;77:4189-99 pubmed publisher
    ..in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize trehalose could improve the acid tolerance of this organism...
  19. Abicht H, Martinez J, Layer G, Jahn D, Solioz M. Lactococcus lactis HemW (HemN) is a haem-binding protein with a putative role in haem trafficking. Biochem J. 2012;442:335-43 pubmed publisher
    b>Lactococcus lactis cannot synthesize haem, but when supplied with haem, expresses a cytochrome bd oxidase. Apart from the cydAB structural genes for this oxidase, L...
  20. Visweswaran G, Steen A, Leenhouts K, Szeliga M, Ruban B, Hesseling Meinders A, et al. AcmD, a homolog of the major autolysin AcmA of Lactococcus lactis, binds to the cell wall and contributes to cell separation and autolysis. PLoS ONE. 2013;8:e72167 pubmed publisher
    b>Lactococcus lactis expresses the homologous glucosaminidases AcmB, AcmC, AcmA and AcmD. The latter two have three C-terminal LysM repeats for peptidoglycan binding...
  21. Castro Nallar E, Chen H, Gladman S, Moore S, Seemann T, Powell I, et al. Population genomics and phylogeography of an Australian dairy factory derived lytic bacteriophage. Genome Biol Evol. 2012;4:382-93 pubmed publisher
    ..we present the full genomic sequences and evolutionary analyses of a serially sampled population of 28 Lactococcus lactis-infecting phage belonging to the 936-like group in Australia...
  22. Knerr P, Oman T, Garcia De Gonzalo C, Lupoli T, Walker S, van der Donk W. Non-proteinogenic amino acids in lacticin 481 analogues result in more potent inhibition of peptidoglycan transglycosylation. ACS Chem Biol. 2012;7:1791-5 pubmed publisher
    ..Additionally, we demonstrate that lacticin 481 does not form pores in the membranes of susceptible bacteria, a common mode-of-action of other lantibiotics. ..
  23. Pontes D, de Azevedo M, Chatel J, Langella P, Azevedo V, Miyoshi A. Lactococcus lactis as a live vector: heterologous protein production and DNA delivery systems. Protein Expr Purif. 2011;79:165-75 pubmed publisher
    ..b>Lactococcus lactis, a LAB species, is a potential candidate for the production of biologically useful proteins and for plasmid ..
  24. To T, Grandvalet C, Tourdot Maréchal R. Cyclopropanation of membrane unsaturated fatty acids is not essential to the acid stress response of Lactococcus lactis subsp. cremoris. Appl Environ Microbiol. 2011;77:3327-34 pubmed publisher
    ..The role of CFAs in the acid tolerance response was investigated in the lactic acid bacterium Lactococcus lactis MG1363. A mutant of the cfa gene was constructed by allelic exchange...
  25. Salonen N, Nyyssölä A, Salonen K, Turunen O. Bifidobacterium longum L-arabinose isomerase--overexpression in Lactococcus lactis, purification, and characterization. Appl Biochem Biotechnol. 2012;168:392-405 pubmed
    Bifidobacterium longum NRRL B-41409 L-arabinose isomerase (L-AI) was cloned and overexpressed in Lactococcus lactis using a phosphate-depletion-inducible expression system. The purified B...
  26. Carvalho A, Turner D, Fonseca L, Solopova A, Catarino T, Kuipers O, et al. Metabolic and transcriptional analysis of acid stress in Lactococcus lactis, with a focus on the kinetics of lactic acid pools. PLoS ONE. 2013;8:e68470 pubmed publisher
    ..A qualitative model for pH effects and acid stress adaptation is proposed on the basis of these results. ..
  27. Bachmann H, Starrenburg M, Molenaar D, Kleerebezem M, van Hylckama Vlieg J. Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution. Genome Res. 2012;22:115-24 pubmed publisher
    ..The lactic acid bacterium Lactococcus lactis naturally occurs on plants and in dairy environments, and it is proposed that dairy strains originate from ..
  28. Jia G, Stephanopoulos G, Gunawan R. Parameter estimation of kinetic models from metabolic profiles: two-phase dynamic decoupling method. Bioinformatics. 2011;27:1964-70 pubmed publisher
    ..of this two-phase method was tested on a generic branched metabolic pathway and the glycolytic pathway of Lactococcus lactis. The results showed that the method is efficient in getting accurate parameter estimates, even when some ..
  29. Oman T, Lupoli T, Wang T, Kahne D, Walker S, van der Donk W. Haloduracin ? binds the peptidoglycan precursor lipid II with 2:1 stoichiometry. J Am Chem Soc. 2011;133:17544-7 pubmed publisher
    ..Combined with previous findings, the data support a model in which a 1:2:2 lipid II:Hal?:Hal? complex inhibits cell wall biosynthesis and mediates pore formation, resulting in loss of membrane potential and potassium efflux...
  30. Zhang H, Qiu Y, Zhao Y, Liu X, Liu M, Yu A. Immunogenicity of oral vaccination with Lactococcus lactis derived vaccine candidate antigen (UreB) of Helicobacter pylori fused with the human interleukin 2 as adjuvant. Mol Cell Probes. 2014;28:25-30 pubmed publisher
    ..pylori infections. By using recombinant technology, Lactococcus lactis (L. lactis) could serve as an antigen-delivering vehicle for the development of edible vaccine...
  31. Kütahya O, Starrenburg M, Rademaker J, Klaassen C, van Hylckama Vlieg J, Smid E, et al. High-resolution amplified fragment length polymorphism typing of Lactococcus lactis strains enables identification of genetic markers for subspecies-related phenotypes. Appl Environ Microbiol. 2011;77:5192-8 pubmed publisher
    ..fragment length polymorphism (AFLP) methodology was developed to achieve the delineation of closely related Lactococcus lactis strains...
  32. Machii M, Watanabe S, Zendo T, Chibazakura T, Sonomoto K, Shimizu Kadota M, et al. Chemically defined media and auxotrophy of the prolific l-lactic acid producer Lactococcus lactis IO-1. J Biosci Bioeng. 2013;115:481-4 pubmed publisher
    ..media, CDM-1G and CDM-1X, that use glucose and xylose as carbon sources, respectively, were prepared for Lactococcus lactis strain IO-1. The maximal cell density at 600 nm in CDM-1G exceeded 2...
  33. Le D, Zanna S, Frateur I, Marcus P, Loubiere P, Dague E, et al. Real-time investigation of the muco-adhesive properties of Lactococcus lactis using a quartz crystal microbalance with dissipation monitoring. Biofouling. 2012;28:479-90 pubmed publisher
    This work was devoted to probe, at the entire population level, interactions between mucins and Lactococcus lactis, using QCM-D. Real-time monitoring of adsorption on polystyrene of PGM (Pig Gastric Mucin) and subsequent adhesion of L...
  34. Rossi P, Barbieri C, Aramini J, Bini E, Lee H, Janjua H, et al. Structures of apo- and ssDNA-bound YdbC from Lactococcus lactis uncover the function of protein domain family DUF2128 and expand the single-stranded DNA-binding domain proteome. Nucleic Acids Res. 2013;41:2756-68 pubmed publisher
    ..b>Lactococcus lactis YdbC is a representative of DUF2128...
  35. Millen A, Horvath P, Boyaval P, Romero D. Mobile CRISPR/Cas-mediated bacteriophage resistance in Lactococcus lactis. PLoS ONE. 2012;7:e51663 pubmed publisher
    b>Lactococcus lactis is a biotechnological workhorse for food fermentations and potentially therapeutic products and is therefore widely consumed by humans...
  36. Mu D, Montalbán López M, Masuda Y, Kuipers O. Zirex: a novel zinc-regulated expression system for Lactococcus lactis. Appl Environ Microbiol. 2013;79:4503-8 pubmed publisher
    Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis...
  37. Broadbent J, Brighton C, McMahon D, Farkye N, Johnson M, Steele J. Microbiology of Cheddar cheese made with different fat contents using a Lactococcus lactis single-strain starter. J Dairy Sci. 2013;96:4212-22 pubmed publisher
    ..containing <6% fat) Cheddar cheeses were manufactured at 3 different locations with a single-strain Lactococcus lactis starter culture using standardized procedures...
  38. Lei H, Sheng Z, Ding Q, Chen J, Wei X, Lam D, et al. Evaluation of oral immunization with recombinant avian influenza virus HA1 displayed on the Lactococcus lactis surface and combined with the mucosal adjuvant cholera toxin subunit B. Clin Vaccine Immunol. 2011;18:1046-51 pubmed publisher
    ..In this study, we constructed a recombinant (pgsA-HA1 gene fusion) Lactococcus lactis strain that expresses and displays the avian influenza virus HA1 antigens on its surface...
  39. Adamberg K, Seiman A, Vilu R. Increased biomass yield of Lactococcus lactis by reduced overconsumption of amino acids and increased catalytic activities of enzymes. PLoS ONE. 2012;7:e48223 pubmed publisher
    ..proteome, and transcriptome) are used to identify parameters that control the increase in biomass yield of Lactococcus lactis from 0.10 to 0.12 C-mol C-mol(-1) with an increase in specific growth rate by 5 times from 0.1 to 0.5 h(-1)...
  40. Bahey El Din M. Lactococcus lactis-based vaccines from laboratory bench to human use: an overview. Vaccine. 2012;30:685-90 pubmed publisher
    ..Several vaccine delivery vectors have been investigated among which the generally regarded as safe (GRAS) Lactococcus lactis has a distinguished position. In this review, different factors affecting the efficacy of L...
  41. Tong Z, Zhou L, Li J, Kuang R, Lin Y, Ni L. An in vitro investigation of Lactococcus lactis antagonizing cariogenic bacterium Streptococcus mutans. Arch Oral Biol. 2012;57:376-82 pubmed publisher
    The present study tested the antagonism between Lactococcus lactis and Streptococcus mutans and evaluated the feasibility of the application of L. lactis for the inhibition of S. mutans in the oral cavity.
  42. Rodríguez Rubio L, Gutiérrez D, Martinez B, Rodriguez A, Garcia P. Lytic activity of LysH5 endolysin secreted by Lactococcus lactis using the secretion signal sequence of bacteriocin Lcn972. Appl Environ Microbiol. 2012;78:3469-72 pubmed publisher
    ..endolysin LysH5, encoded by Staphylococcus aureus phage vB_SauS-phi-IPLA88, was expressed and secreted in Lactococcus lactis using the signal peptide of bacteriocin lactococcin 972 and lactococcal constitutive and inducible promoters...
  43. Quiroga C, Kronstad L, Ritlop C, Filion A, Cousineau B. Contribution of base-pairing interactions between group II intron fragments during trans-splicing in vivo. RNA. 2011;17:2212-21 pubmed publisher
    ..The Ll.LtrB group II intron from the Gram-positive bacterium Lactococcus lactis was shown to splice in trans when fragmented at various locations throughout its structure. Here we used Ll...
  44. Moisan M, Moineau S. Multilocus sequence typing scheme for the characterization of 936-like phages infecting Lactococcus lactis. Appl Environ Microbiol. 2012;78:4646-53 pubmed publisher
    b>Lactococcus lactis phage infections are costly for the dairy industry because they can slow down the fermentation process and adversely impact product safety and quality...
  45. Lamarque M, Aubel D, Piard J, Gilbert C, Juillard V, Atlan D. The peptide transport system Opt is involved in both nutrition and environmental sensing during growth of Lactococcus lactis in milk. Microbiology. 2011;157:1612-9 pubmed publisher
    b>Lactococcus lactis is known to take up extracellular peptides via at least three distinct peptide transporters. The well-described oligopeptide transporter Opp alone is able to ensure the growth of L...
  46. Solopova A, Bachmann H, Teusink B, Kok J, Neves A, Kuipers O. A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363. Appl Environ Microbiol. 2012;78:5612-21 pubmed publisher
    The Lactococcus lactis laboratory strain MG1363 has been described to be unable to utilize lactose. However, in a rich medium supplemented with lactose as the sole carbon source, it starts to grow after prolonged incubation periods...
  47. Sawai H, Yamanaka M, Sugimoto H, Shiro Y, Aono S. Structural basis for the transcriptional regulation of heme homeostasis in Lactococcus lactis. J Biol Chem. 2012;287:30755-68 pubmed publisher
    ..Numerous lactic acid bacteria, including Lactococcus lactis, acquire heme molecules exogenously to establish an aerobic respiratory chain...
  48. Shigemori S, Yonekura S, Sato T, Otani H, Shimosato T. Expression of the immunoreactive buckwheat major allergenic storage protein in Lactococcus lactis. Appl Microbiol Biotechnol. 2013;97:3603-11 pubmed publisher
    ..study, we successfully developed a modified lactic acid bacterial vector (pNSH) and a recombinant strain of Lactococcus lactis NZ9000 (NZ9000) that produced a major allergenic storage protein of buckwheat, Fagag1 (61...
  49. Papagianni M, Avramidis N. Lactococcus lactis as a cell factory: a twofold increase in phosphofructokinase activity results in a proportional increase in specific rates of glucose uptake and lactate formation. Enzyme Microb Technol. 2011;49:197-202 pubmed publisher
    Despite the fact that the area of glycolysis in Lactococcus lactis has been intensively studied, only a limited number of studies have been focused on the regulation of uptake of glucose itself...
  50. Picard F, Loubiere P, Girbal L, Cocaign Bousquet M. The significance of translation regulation in the stress response. BMC Genomics. 2013;14:588 pubmed publisher
  51. Agustiandari H, Peeters E, de Wit J, Charlier D, Driessen A. LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis. Microbiology. 2011;157:1519-30 pubmed publisher
    Multidrug resistance (MDR) in Lactococcus lactis is due to the expression of the membrane ATP-binding cassette (ABC) transporter LmrCD...
  52. Lu W, Kong J, Kong W. Construction and application of a food-grade expression system for Lactococcus lactis. Mol Biotechnol. 2013;54:170-6 pubmed publisher
    A food-grade host/vector expression system for Lactococcus lactis was constructed using alanine racemase gene (alr) as the complementation marker. We obtained an alanine racemase auxotrophic mutant L...
  53. Xu Y, Kong J, Kong W. Improved membrane protein expression in Lactococcus lactis by fusion to Mistic. Microbiology. 2013;159:1002-9 pubmed publisher
    ..b>Lactococcus lactis possesses many properties ideal for membrane protein expression...
  54. De Jong A, Hansen M, Kuipers O, Kilstrup M, Kok J. The transcriptional and gene regulatory network of Lactococcus lactis MG1363 during growth in milk. PLoS ONE. 2013;8:e53085 pubmed publisher
    In the present study we examine the changes in the expression of genes of Lactococcus lactis subspecies cremoris MG1363 during growth in milk...
  55. de Haan A, Haijema B, Voorn P, Meijerhof T, van Roosmalen M, Leenhouts K. Bacterium-like particles supplemented with inactivated influenza antigen induce cross-protective influenza-specific antibody responses through intranasal administration. Vaccine. 2012;30:4884-91 pubmed publisher
    ..e. bacterium-like particles (BLPs) derived from the food-grade bacterium in Lactococcus lactis, in the induction of protective antibody responses after intranasal immunization of mice...
  56. Guo T, Kong J, Zhang L, Zhang C, Hu S. Fine tuning of the lactate and diacetyl production through promoter engineering in Lactococcus lactis. PLoS ONE. 2012;7:e36296 pubmed publisher
    b>Lactococcus lactis is a well-studied bacterium widely used in dairy fermentation and capable of producing metabolites with organoleptic and nutritional characteristics...
  57. van Pijkeren J, Neoh K, Sirias D, Findley A, Britton R. Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri. Bioengineered. 2012;3:209-17 pubmed publisher
    ..By in silico analysis we identified ssDNA binding protein homologs in the genus Lactobacillus and Lactococcus lactis. To assess whether we could further improve the recombineering efficiency in Lactobacillus reuteri ATCC PTA ..
  58. Gaspar P, Neves A, Gasson M, Shearman C, Santos H. High yields of 2,3-butanediol and mannitol in Lactococcus lactis through engineering of NAD? cofactor recycling. Appl Environ Microbiol. 2011;77:6826-35 pubmed publisher
    ..of NADH-dependent steps, and particularly disruption of the las-located lactate dehydrogenase (ldh) gene in Lactococcus lactis, is common to engineering strategies envisaging the accumulation of reduced end products other than lactate...
  59. Fallico V, McAuliffe O, Fitzgerald G, Ross R. Plasmids of raw milk cheese isolate Lactococcus lactis subsp. lactis biovar diacetylactis DPC3901 suggest a plant-based origin for the strain. Appl Environ Microbiol. 2011;77:6451-62 pubmed publisher
    The four-plasmid complement of the raw milk cheese isolate Lactococcus lactis subsp. lactis biovar diacetylactis DPC3901 was sequenced, and some genetic features were functionally analyzed...
  60. Khusainov R, Kuipers O. When the leader gets loose: in vivo biosynthesis of a leaderless prenisin is stimulated by a trans-acting leader peptide. Chembiochem. 2012;13:2433-8 pubmed publisher
  61. Yam K, Hugentobler F, Pouliot P, Stern A, Lalande J, Matlashewski G, et al. Generation and evaluation of A2-expressing Lactococcus lactis live vaccines against Leishmania donovani in BALB/c mice. J Med Microbiol. 2011;60:1248-60 pubmed publisher
    ..efficacy of Leishmania vaccines engineered from the non-colonizing, non-pathogenic Gram-positive bacterium Lactococcus lactis. A truncated, codon-optimized version of the A2 antigen from Leishmania donovani was engineered for ..
  62. Gao Y, Lu Y, Teng K, Chen M, Zheng H, Zhu Y, et al. Complete genome sequence of Lactococcus lactis subsp. lactis CV56, a probiotic strain isolated from the vaginas of healthy women. J Bacteriol. 2011;193:2886-7 pubmed publisher
    ..Here, we report the finished and annotated genome of a Lactococcus strain that was isolated from the vaginas of healthy women and shows probiotic properties, including nisin A production and adhesion to vaginal epithelial cells...
  63. Piper C, Hill C, Cotter P, Ross R. Bioengineering of a Nisin A-producing Lactococcus lactis to create isogenic strains producing the natural variants Nisin F, Q and Z. Microb Biotechnol. 2011;4:375-82 pubmed publisher
    ..Nisins A, Z, F and Q are produced by Lactococcus lactis, while Nisins U and U2 are produced by Streptococcus sp...
  64. De Jong A, Pietersma H, Cordes M, Kuipers O, Kok J. PePPER: a webserver for prediction of prokaryote promoter elements and regulons. BMC Genomics. 2012;13:299 pubmed publisher
    ..Improved prediction and comparison algorithms are currently available for identifying transcription factor binding sites (TFBSs) and their accompanying TFs and regulon members...
  65. Passerini D, Laroute V, Coddeville M, Le Bourgeois P, Loubiere P, Ritzenthaler P, et al. New insights into Lactococcus lactis diacetyl- and acetoin-producing strains isolated from diverse origins. Int J Food Microbiol. 2013;160:329-36 pubmed publisher
    b>Lactococcus lactis subsp. lactis biovar diacetylactis strains are used in the dairy industry for generating acetoin and notably diacetyl which imparts a high level of buttery flavor notes...
  66. Zadravec P, Mavrič A, Bogovic Matijasić B, Strukelj B, Berlec A. Engineering BmpA as a carrier for surface display of IgG-binding domain on Lactococcus lactis. Protein Eng Des Sel. 2014;27:21-7 pubmed publisher
    ..membrane protein A (BmpA) is a potential carrier protein for surface display of the IgG-binding domain on Lactococcus lactis. We have shown that it can increase the adhesion of bacteria to the intestinal cell model by 1...
  67. Sugimura T, Jounai K, Ohshio K, Tanaka T, Suwa M, Fujiwara D. Immunomodulatory effect of Lactococcus lactis JCM5805 on human plasmacytoid dendritic cells. Clin Immunol. 2013;149:509-18 pubmed publisher
    ..In this study, we demonstrated that Lactococcus lactis JCM5805 activates human pDCs and induces IFN production in vitro...
  68. Medina M, Vintiñi E, Villena J, Raya R, Alvarez S. Lactococcus lactis as an adjuvant and delivery vehicle of antigens against pneumococcal respiratory infections. Bioeng Bugs. 2010;1:313-25 pubmed publisher
    Most studies of Lactococcus lactis as delivery vehicles of pneumococcal antigens are focused on the effectiveness of mucosal recombinant vaccines against Streptococcus pneumoniae in animal models...
  69. Rezende R, Oliveira R, Medeiros S, Gomes Santos A, Alves A, Loli F, et al. Hsp65-producing Lactococcus lactis prevents experimental autoimmune encephalomyelitis in mice by inducing CD4+LAP+ regulatory T cells. J Autoimmun. 2013;40:45-57 pubmed publisher
    ..In this study, we showed that oral administration of a recombinant Lactococcus lactis strain that produces and releases LPS-free Hsp65 prevented the development of experimental autoimmune ..
  70. Lechardeur D, Cesselin B, Liebl U, Vos M, Fernandez A, Brun C, et al. Discovery of intracellular heme-binding protein HrtR, which controls heme efflux by the conserved HrtB-HrtA transporter in Lactococcus lactis. J Biol Chem. 2012;287:4752-8 pubmed publisher
    ..Our previous studies in the Gram-positive bacterium Lactococcus lactis showed that heme exposure strongly induced expression of a single operon, called here hrtRBA, encoding an ..
  71. Sáez D, Fernandez P, Rivera A, Andrews E, Oñate A. Oral immunization of mice with recombinant Lactococcus lactis expressing Cu,Zn superoxide dismutase of Brucella abortus triggers protective immunity. Vaccine. 2012;30:1283-90 pubmed publisher
    ..Here, we evaluated the usefulness of recombinant Lactococcus lactis secreting Brucella abortus Cu-Zn superoxide dismutase (SOD) as oral antigen delivery system, when ..
  72. Takiishi T, Korf H, Van Belle T, Robert S, Grieco F, Caluwaerts S, et al. Reversal of autoimmune diabetes by restoration of antigen-specific tolerance using genetically modified Lactococcus lactis in mice. J Clin Invest. 2012;122:1717-25 pubmed publisher
    ..we developed a strategy for tolerance restoration using mucosal delivery in mice of biologically contained Lactococcus lactis genetically modified to secrete the whole proinsulin autoantigen along with the immunomodulatory cytokine IL-..
  73. Smith W, Pham T, Lei L, Dou J, Soomro A, Beatson S, et al. Heat resistance and salt hypersensitivity in Lactococcus lactis due to spontaneous mutation of llmg_1816 (gdpP) induced by high-temperature growth. Appl Environ Microbiol. 2012;78:7753-9 pubmed publisher
    During construction of several gene deletion mutants in Lactococcus lactis MG1363 which involved a high-temperature (37...
  74. de Azevedo M, Karczewski J, Lefèvre F, Azevedo V, Miyoshi A, Wells J, et al. In vitro and in vivo characterization of DNA delivery using recombinant Lactococcus lactis expressing a mutated form of L. monocytogenes Internalin A. BMC Microbiol. 2012;12:299 pubmed publisher
    ..We previously showed that either native Lactococcus lactis (LL) or recombinant invasive LL expressing Fibronectin Binding Protein A of Staphylococcus aureus (LL-FnBPA+)..
  75. Dressaire C, Picard F, Redon E, Loubiere P, Queinnec I, Girbal L, et al. Role of mRNA stability during bacterial adaptation. PLoS ONE. 2013;8:e59059 pubmed publisher
    ..A genome-wide transcriptomic-based method was used to determine mRNA half-lives. The model bacterium Lactococcus lactis was used and different growth rates were studied in continuous cultures under isoleucine-limitation and in ..
  76. Samson J, Spinelli S, Cambillau C, Moineau S. Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type III toxin-antitoxin system. Mol Microbiol. 2013;87:756-68 pubmed publisher
    AbiQ is a phage resistance mechanism found on a native plasmid of Lactococcus lactis that abort virulent phage infections...
  77. Zhang G, Liu J, Ding W. Decreased xylitol formation during xylose fermentation in Saccharomyces cerevisiae due to overexpression of water-forming NADH oxidase. Appl Environ Microbiol. 2012;78:1081-6 pubmed publisher
    ..To solve this problem, noxE, encoding a water-forming NADH oxidase from Lactococcus lactis driven by the PGK1 promoter, was introduced into the xylose-utilizing yeast strain KAM-3X...
  78. Marelli B, Perez A, Banchio C, de Mendoza D, Magni C. Oral immunization with live Lactococcus lactis expressing rotavirus VP8 subunit induces specific immune response in mice. J Virol Methods. 2011;175:28-37 pubmed publisher
    ..The development of a mucosal live vaccine using the food-grade lactic acid bacteria Lactococcus lactis as antigen vehicle is an attractive and safe vaccination strategy against rotavirus...
  79. Khusainov R, Heils R, Lubelski J, Moll G, Kuipers O. Determining sites of interaction between prenisin and its modification enzymes NisB and NisC. Mol Microbiol. 2011;82:706-18 pubmed publisher
    ..Here, we demonstrate that the nisin modification enzymes NisB and NisC can be pulled down in vitro from Lactococcus lactis by an engineered His-tagged prenisin...
  80. Wang Y, Wang J, Dai W. Use of GFP to trace the colonization of Lactococcus lactis WH-C1 in the gastrointestinal tract of mice. J Microbiol Methods. 2011;86:390-2 pubmed publisher
    ..as a selection marker and GFP as a reporter protein to explore the colonization characteristics in vivo of Lactococcus lactis WH-C1...
  81. Flahaut N, Wiersma A, van de Bunt B, Martens D, Schaap P, Sijtsma L, et al. Genome-scale metabolic model for Lactococcus lactis MG1363 and its application to the analysis of flavor formation. Appl Microbiol Biotechnol. 2013;97:8729-39 pubmed publisher
    b>Lactococcus lactis subsp. cremoris MG1363 is a paradigm strain for lactococci used in industrial dairy fermentations. However, despite of its importance for process development, no genome-scale metabolic model has been reported thus far...
  82. Douillard F, Mahony J, Campanacci V, Cambillau C, van Sinderen D. Construction of two Lactococcus lactis expression vectors combining the Gateway and the NIsin Controlled Expression systems. Plasmid. 2011;66:129-35 pubmed publisher
    ..years, the NIsin Controlled Expression (NICE) system has been extensively used in the food-grade bacterium Lactococcus lactis subsp. cremoris to produce homologous and heterologous proteins for academic and biotechnological purposes...
  83. Siezen R, Bayjanov J, Felis G, van der Sijde M, Starrenburg M, Molenaar D, et al. Genome-scale diversity and niche adaptation analysis of Lactococcus lactis by comparative genome hybridization using multi-strain arrays. Microb Biotechnol. 2011;4:383-402 pubmed publisher
    b>Lactococcus lactis produces lactic acid and is widely used in the manufacturing of various fermented dairy products. However, the species is also frequently isolated from non-dairy niches, such as fermented plant material...
  84. Ustün O, Ongen G. Production and separation of dipeptidyl peptidase IV from Lactococcus lactis: scale up for industrial production. Bioprocess Biosyst Eng. 2012;35:1417-27 pubmed publisher
    b>Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris are widely used in the manufacture of fermented milk...
  85. Taïbi A, Dabour N, Lamoureux M, Roy D, LaPointe G. Comparative transcriptome analysis of Lactococcus lactis subsp. cremoris strains under conditions simulating Cheddar cheese manufacture. Int J Food Microbiol. 2011;146:263-75 pubmed publisher
    ..The strain-specific responses identify predictive markers for the transcriptional state of starter strains before they enter the cheese ripening phase...
  86. Zuercher A, Weiss M, Holvoet S, Moser M, Moussu H, Van Overtvelt L, et al. Lactococcus lactis NCC 2287 alleviates food allergic manifestations in sensitized mice by reducing IL-13 expression specifically in the ileum. Clin Dev Immunol. 2012;2012:485750 pubmed publisher
    Utilizing a food allergy murine model, we have investigated the intrinsic antiallergic potential of the Lactococcus lactis NCC 2287 strain.
  87. Dressaire C, Redon E, Gitton C, Loubiere P, Monnet V, Cocaign Bousquet M. Investigation of the adaptation of Lactococcus lactis to isoleucine starvation integrating dynamic transcriptome and proteome information. Microb Cell Fact. 2011;10 Suppl 1:S18 pubmed publisher
    ..The global response of the LAB model Lactococcus lactis ssp...
  88. Veesler D, Spinelli S, Mahony J, Lichière J, Blangy S, Bricogne G, et al. Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism. Proc Natl Acad Sci U S A. 2012;109:8954-8 pubmed publisher
    ..These data suggest that these two families rely on diverse adhesion strategies which may lead to different signaling for genome release...
  89. Goel A, Santos F, Vos W, Teusink B, Molenaar D. Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions. Appl Environ Microbiol. 2012;78:134-43 pubmed publisher
    ..Current kinetic parameters for Lactococcus lactis are scattered through different studies and performed under different assay conditions...
  90. Shigemori S, Yonekura S, Sato T, Nakanishi M, Otani H, Shimosato T. Expression of a biologically active GFP-?(S1)-casein fusion protein in Lactococcus lactis. Curr Microbiol. 2012;64:569-75 pubmed publisher
    In this study, we successfully developed a recombinant strain of Lactococcus lactis NZ9000 (NZ9000) that produced green fluorescent protein fused to ?(S1)-casein (GFP-?(S1)Cas)...