haemophilus ducreyi

Summary

Summary: A species of HAEMOPHILUS that appears to be the pathogen or causative agent of the sexually transmitted disease, CHANCROID.

Top Publications

  1. Thomas C, Olsen B, Elkins C. Cloning and characterization of tdhA, a locus encoding a TonB-dependent heme receptor from Haemophilus ducreyi. Infect Immun. 1998;66:4254-62 pubmed
    b>Haemophilus ducreyi is unable to synthesize heme and must acquire it from its only known host, humans. We cloned and sequenced a gene encoding an outer membrane receptor for heme...
  2. Munson R, Zhong H, Mungur R, Ray W, Shea R, Mahairas G, et al. Haemophilus ducreyi strain ATCC 27722 contains a genetic element with homology to the vibrio RS1 element that can replicate as a plasmid and confer NAD independence on haemophilus influenzae. Infect Immun. 2004;72:1143-6 pubmed
    The nucleotide sequence of pNAD1, a plasmid from Haemophilus ducreyi identified on the basis of its ability to confer NAD independence on Actinobacillus pleuropneumoniae and H. influenzae, has been determined...
  3. Bauer M, Goheen M, Townsend C, Spinola S. Haemophilus ducreyi associates with phagocytes, collagen, and fibrin and remains extracellular throughout infection of human volunteers. Infect Immun. 2001;69:2549-57 pubmed
    In a previous study, Haemophilus ducreyi was found in the pustule and dermis of samples obtained at the clinical end point in the human model of infection...
  4. Bong C, Bauer M, Spinola S. Haemophilus ducreyi: clinical features, epidemiology, and prospects for disease control. Microbes Infect. 2002;4:1141-8 pubmed
    b>Haemophilus ducreyi is the causative agent of the genital ulcer disease chancroid. Chancroid is common in developing countries and facilitates human immunodeficiency virus transmission...
  5. Chaffin D, McKinnon K, Rubens C. CpsK of Streptococcus agalactiae exhibits alpha2,3-sialyltransferase activity in Haemophilus ducreyi. Mol Microbiol. 2002;45:109-22 pubmed
    ..of most serotypes, which was homologous to the lipooligosaccharide (LOS) sialyltransferase gene, lst, of Haemophilus ducreyi. To determine if cpsK might encode a sialyltransferase, we complemented a H. ducreyi lst mutant with cpsK...
  6. Wood G, Dutro S, Totten P. Haemophilus ducreyi inhibits phagocytosis by U-937 cells, a human macrophage-like cell line. Infect Immun. 2001;69:4726-33 pubmed
    b>Haemophilus ducreyi is a gram-negative obligate human pathogen that causes the genital ulcer disease chancroid. Chancroid lesions are deep necrotic ulcers with an immune cell infiltrate that includes macrophages...
  7. Spinola S, Bong C, Faber A, Fortney K, Bennett S, Townsend C, et al. Differences in host susceptibility to disease progression in the human challenge model of Haemophilus ducreyi infection. Infect Immun. 2003;71:6658-63 pubmed
    With human volunteers inoculated at two sites with Haemophilus ducreyi, outcomes for a subject were not independent. In a reinfection trial, 2 of 11 previous pustule formers and 6 of 10 previous resolvers resolved all sites of infection...
  8. White C, Leduc I, Olsen B, Jeter C, Harris C, Elkins C. Haemophilus ducreyi Outer membrane determinants, including DsrA, define two clonal populations. Infect Immun. 2005;73:2387-99 pubmed
    The Haemophilus ducreyi outer membrane component DsrA (for ducreyi serum resistance A) is necessary for complete resistance to normal human serum (NHS)...
  9. Banks K, Fortney K, Baker B, Billings S, Katz B, Munson R, et al. The enterobacterial common antigen-like gene cluster of Haemophilus ducreyi contributes to virulence in humans. J Infect Dis. 2008;197:1531-6 pubmed publisher
    b>Haemophilus ducreyi 35000HP contains a cluster of homologues of genes required for the synthesis of enterobacterial common antigen (ECA), suggesting that H. ducreyi may express a putative ECA-like glycoconjugate...

More Information

Publications80

  1. Spinola S, Fortney K, Katz B, Latimer J, Mock J, Vakevainen M, et al. Haemophilus ducreyi requires an intact flp gene cluster for virulence in humans. Infect Immun. 2003;71:7178-82 pubmed
    An intact Haemophilus ducreyi flp operon is essential for microcolony formation in vitro. tadA is the 9th of 15 genes in the operon and has homology to NTPases of type IV secretion systems...
  2. Spinola S, Fortney K, Baker B, Janowicz D, Zwickl B, Katz B, et al. Activation of the CpxRA system by deletion of cpxA impairs the ability of Haemophilus ducreyi to infect humans. Infect Immun. 2010;78:3898-904 pubmed publisher
    b>Haemophilus ducreyi must adapt to the environment of the human host to establish and maintain infection in the skin...
  3. Abdullah M, Nepluev I, Afonina G, Ram S, Rice P, Cade W, et al. Killing of dsrA mutants of Haemophilus ducreyi by normal human serum occurs via the classical complement pathway and is initiated by immunoglobulin M binding. Infect Immun. 2005;73:3431-9 pubmed
    Previously, we showed that serum resistance in Haemophilus ducreyi type strain 35000HP required expression of the outer membrane protein DsrA because the isogenic dsrA mutant FX517 is highly serum susceptible...
  4. Bozue J, Tullius M, Wang J, Gibson B, Munson R. Haemophilus ducreyi produces a novel sialyltransferase. Identification of the sialyltransferase gene and construction of mutants deficient in the production of the sialic acid-containing glycoform of the lipooligosaccharide. J Biol Chem. 1999;274:4106-14 pubmed
    b>Haemophilus ducreyi, the cause of the sexually transmitted disease chancroid produces a lipooligosaccharide (LOS) containing a terminal sialyl N-acetyllactosamine trisaccharide...
  5. Martin P, Shea R, Mulks M. Identification of a plasmid-encoded gene from Haemophilus ducreyi which confers NAD independence. J Bacteriol. 2001;183:1168-74 pubmed publisher
    ..2-kb plasmid which was previously shown to be responsible for NAD independence in Haemophilus ducreyi. When transformed into A...
  6. Wising C, Mölne L, Jonsson I, Ahlman K, Lagergard T. The cytolethal distending toxin of Haemophilus ducreyi aggravates dermal lesions in a rabbit model of chancroid. Microbes Infect. 2005;7:867-74 pubmed
    b>Haemophilus ducreyi, the etiologic agent of the sexually transmitted disease chancroid, produces a cytolethal distending toxin (HdCDT) that inhibits cultured cell proliferation, leading to cell death...
  7. Samuels N, Gibson B, Miller S. Investigation of the kinetic mechanism of cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from Haemophilus ducreyi with new insights on rate-limiting steps from product inhibition analysis. Biochemistry. 1999;38:6195-203 pubmed
    ..role of sialic acid in the pathobiology of these organisms, we have initiated studies of the enzymes from Haemophilus ducreyi (the infectious agent of chancroid) responsible for the activation and attachment of sialic acid to the ..
  8. Bauer B, Stevens M, Hansen E. Involvement of the Haemophilus ducreyi gmhA gene product in lipooligosaccharide expression and virulence. Infect Immun. 1998;66:4290-8 pubmed
    The lipooligosaccharide (LOS) present in the outer membrane of Haemophilus ducreyi is likely a virulence factor for this sexually transmitted pathogen. An open reading frame in H...
  9. Tullius M, Munson R, Wang J, Gibson B. Purification, cloning, and expression of a cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from Haemophilus ducreyi. J Biol Chem. 1996;271:15373-80 pubmed
    An N-acetylneuraminic acid cytidylyltransferase (EC 2.7.7.43) (CMP-NeuAc synthetase) was isolated from a Haemophilus ducreyi strain 35000 cell lysate and partially characterized...
  10. Janowicz D, Fortney K, Katz B, Latimer J, Deng K, Hansen E, et al. Expression of the LspA1 and LspA2 proteins by Haemophilus ducreyi is required for virulence in human volunteers. Infect Immun. 2004;72:4528-33 pubmed
    b>Haemophilus ducreyi colocalizes with polymorphonuclear leukocytes and macrophages and evades phagocytosis during experimental infection of human volunteers. H...
  11. Palmer K, Munson R. Cloning and characterization of the genes encoding the hemolysin of Haemophilus ducreyi. Mol Microbiol. 1995;18:821-30 pubmed
    We previously identified a heat- and protease-labile haemolytic activity expressed by Haemophilus ducreyi. In order to characterize the haemolysin at the molecular level, genomic DNA from H...
  12. Guerra L, Nemec K, Massey S, Tatulian S, Thelestam M, Frisan T, et al. A novel mode of translocation for cytolethal distending toxin. Biochim Biophys Acta. 2009;1793:489-95 pubmed publisher
    ..The Haemophilus ducreyi cytolethal distending toxin (HdCDT) does not utilize ERAD to exit the ER, so we predicted the structural ..
  13. Janowicz D, Li W, Bauer M. Host-pathogen interplay of Haemophilus ducreyi. Curr Opin Infect Dis. 2010;23:64-9 pubmed publisher
    b>Haemophilus ducreyi, the causative agent of the sexually transmitted infection chancroid, is primarily a pathogen of human skin. During infection, H...
  14. Spinola S, Bauer M, Munson R. Immunopathogenesis of Haemophilus ducreyi infection (chancroid). Infect Immun. 2002;70:1667-76 pubmed
  15. Labandeira Rey M, Dodd D, Fortney K, Zwickl B, Katz B, Janowicz D, et al. A Haemophilus ducreyi CpxR deletion mutant is virulent in human volunteers. J Infect Dis. 2011;203:1859-65 pubmed publisher
    b>Haemophilus ducreyi 35000HP contains a homolog of the CpxRA 2-component signal transduction system, which controls the cell envelope stress response system in other gram-negative bacteria and regulates some important H...
  16. Schilling B, Goon S, Samuels N, Gaucher S, Leary J, Bertozzi C, et al. Biosynthesis of sialylated lipooligosaccharides in Haemophilus ducreyi is dependent on exogenous sialic acid and not mannosamine. Incorporation studies using N-acylmannosamine analogues, N-glycolylneuraminic acid, and 13C-labeled N-acetylneuraminic a. Biochemistry. 2001;40:12666-77 pubmed
    b>Haemophilus ducreyi is a Gram-negative bacterium that causes chancroid, a sexually transmitted disease. Cell surface lipooligosaccharides (LOS) of H. ducreyi are thought to play important biological roles in host infection...
  17. Svensson L, Tarkowski A, Thelestam M, Lagergard T. The impact of Haemophilus ducreyi cytolethal distending toxin on cells involved in immune response. Microb Pathog. 2001;30:157-66 pubmed
    The Haemophilus ducreyi cytolethal distending toxin (HdCDT) induces cell cycle arrest and thereby inhibits cell proliferation of many cultured mammalian cell-lines...
  18. Fulcher R, Cole L, Janowicz D, Toffer K, Fortney K, Katz B, et al. Expression of Haemophilus ducreyi collagen binding outer membrane protein NcaA is required for virulence in swine and human challenge models of chancroid. Infect Immun. 2006;74:2651-8 pubmed
    b>Haemophilus ducreyi, the etiologic agent of the sexually transmitted genital ulcer disease chancroid, has been shown to associate with dermal collagen fibers within infected skin lesions...
  19. Cao L, Volgina A, Huang C, Korostoff J, DiRienzo J. Characterization of point mutations in the cdtA gene of the cytolethal distending toxin of Actinobacillus actinomycetemcomitans. Mol Microbiol. 2005;58:1303-21 pubmed
    ..The mutations in mutA81 and mutA221 disrupted holotoxin formation. The positions of the amino acid substitutions were mapped in the Haemophilus ducreyi Cdt crystal structure providing some insight into structure and function.
  20. Davie J, Campagnari A. Comparative proteomic analysis of the Haemophilus ducreyi porin-deficient mutant 35000HP::P2AB. J Bacteriol. 2009;191:2144-52 pubmed publisher
    b>Haemophilus ducreyi is an obligate human pathogen and the causative agent of the sexually transmitted, genital ulcerative disease chancroid. The genome of strain 35000HP contains two known porin proteins, OmpP2A and OmpP2B...
  21. Post D, Munson R, Baker B, Zhong H, Bozue J, Gibson B. Identification of genes involved in the expression of atypical lipooligosaccharide structures from a second class of Haemophilus ducreyi. Infect Immun. 2007;75:113-21 pubmed
    b>Haemophilus ducreyi is a gram-negative bacterium that is the causative agent of chancroid. Strain 35000HP has been well characterized and is representative of the majority of H. ducreyi strains...
  22. Janowicz D, Leduc I, Fortney K, Katz B, Elkins C, Spinola S. A DltA mutant of Haemophilus ducreyi Is partially attenuated in its ability to cause pustules in human volunteers. Infect Immun. 2006;74:1394-7 pubmed
    b>Haemophilus ducreyi produces two outer membrane proteins, called DltA (H. ducreyi lectin A) and DsrA (H. ducreyi serum resistance A), that contribute to the ability of the organism to evade complement-mediated serum killing...
  23. Mackay I, Harnett G, Jeoffreys N, Bastian I, Sriprakash K, Siebert D, et al. Detection and discrimination of herpes simplex viruses, Haemophilus ducreyi, Treponema pallidum, and Calymmatobacterium (Klebsiella) granulomatis from genital ulcers. Clin Infect Dis. 2006;42:1431-8 pubmed
    ..This collaborative project was undertaken to address the need for a rapid, economical, and sensitive approach to the detection and diagnosis of GUD using noninvasive techniques to sample genital ulcers...
  24. Guerra L, Teter K, Lilley B, Stenerlow B, Holmes R, Ploegh H, et al. Cellular internalization of cytolethal distending toxin: a new end to a known pathway. Cell Microbiol. 2005;7:921-34 pubmed
    ..However, little is known about their cellular internalization pathway. We demonstrate that binding of the Haemophilus ducreyi CDT (HdCDT) on the plasma membrane of sensitive cells was abolished by cholesterol extraction with methyl-..
  25. Challacombe J, Duncan A, Brettin T, Bruce D, Chertkov O, Detter J, et al. Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd. J Bacteriol. 2007;189:1890-8 pubmed
    ..somnus 129Pt, Haemophilus influenzae Rd, and Haemophilus ducreyi 35000HP. We found unique genes in H...
  26. Mock J, Vakevainen M, Deng K, Latimer J, Young J, van Oers N, et al. Haemophilus ducreyi targets Src family protein tyrosine kinases to inhibit phagocytic signaling. Infect Immun. 2005;73:7808-16 pubmed
    b>Haemophilus ducreyi, the etiologic agent of the sexually transmitted disease chancroid, has been shown to inhibit phagocytosis of both itself and secondary targets in vitro. Immunodepletion of LspA proteins from H...
  27. Tullius M, Phillips N, Scheffler N, Samuels N, Munson Jr R, Hansen E, et al. The lbgAB gene cluster of Haemophilus ducreyi encodes a beta-1,4-galactosyltransferase and an alpha-1,6-DD-heptosyltransferase involved in lipooligosaccharide biosynthesis. Infect Immun. 2002;70:2853-61 pubmed
    All Haemophilus ducreyi strains examined contain a lipooligosaccharide (LOS) consisting of a single but variable branch oligosaccharide that emanates off the first heptose (Hep-I) of a conserved Hep(3)-phosphorylated 3-deoxy-D-manno-..
  28. Peel T, Bhatti D, De Boer J, Stratov I, Spelman D. Chronic cutaneous ulcers secondary to Haemophilus ducreyi infection. Med J Aust. 2010;192:348-50 pubmed
    b>Haemophilus ducreyi is a well recognised causative agent of genital ulcers and chancroid. We report two unusual cases of non-sexually transmitted H. ducreyi infection leading to chronic lower limb ulcers...
  29. Thomas K, Leduc I, Olsen B, Thomas C, Cameron D, Elkins C. Cloning, overexpression, purification, and immunobiology of an 85-kilodalton outer membrane protein from Haemophilus ducreyi. Infect Immun. 2001;69:4438-46 pubmed
    We have identified an 85-kDa outer membrane protein that is expressed by all tested strains of Haemophilus ducreyi. Studies of related proteins from other pathogenic bacteria, including Haemophilus influenzae, Pasteurella multocida, ..
  30. Ward C, Mock J, Hansen E. The LspB protein is involved in the secretion of the LspA1 and LspA2 proteins by Haemophilus ducreyi. Infect Immun. 2004;72:1874-84 pubmed
    The LspA1 and LspA2 proteins of Haemophilus ducreyi 35000 are two very large macromolecules that can be detected in concentrated culture supernatant fluid...
  31. Janowicz D, Ofner S, Katz B, Spinola S. Experimental infection of human volunteers with Haemophilus ducreyi: fifteen years of clinical data and experience. J Infect Dis. 2009;199:1671-9 pubmed publisher
    b>Haemophilus ducreyi causes chancroid, which facilitates transmission of human immunodeficiency virus type 1. To better understand the biology of H. ducreyi, we developed a human inoculation model...
  32. Deng K, Latimer J, Lewis D, Hansen E. Investigation of the interaction among the components of the cytolethal distending toxin of Haemophilus ducreyi. Biochem Biophys Res Commun. 2001;285:609-15 pubmed
    The cytolethal distending toxin (CDT) of Haemophilus ducreyi is encoded by the cdtABC genes, but the composition of active CDT is not known. Both immunoaffinity and metal affinity chromatographic methods were used to purify H...
  33. Gargi A, Tamilselvam B, Powers B, Prouty M, LINCECUM T, Eshraghi A, et al. Cellular interactions of the cytolethal distending toxins from Escherichia coli and Haemophilus ducreyi. J Biol Chem. 2013;288:7492-505 pubmed publisher
    ..Here, the intoxication mechanisms of CDTs from Escherichia coli (Ec-CDT) and Haemophilus ducreyi (Hd-CDT), which share limited amino acid sequence homology, were directly compared...
  34. Cope L, Lumbley S, Latimer J, Klesney Tait J, Stevens M, Johnson L, et al. A diffusible cytotoxin of Haemophilus ducreyi. Proc Natl Acad Sci U S A. 1997;94:4056-61 pubmed
    Little is known about the virulence mechanisms employed by Haemophilus ducreyi in the production of genital ulcers...
  35. Vakevainen M, Greenberg S, Hansen E. Inhibition of phagocytosis by Haemophilus ducreyi requires expression of the LspA1 and LspA2 proteins. Infect Immun. 2003;71:5994-6003 pubmed
    b>Haemophilus ducreyi previously has been shown to inhibit the phagocytosis of both secondary targets and itself by certain cells in vitro. Wild-type H...
  36. Brentjens R, Ketterer M, Apicella M, Spinola S. Fine tangled pili expressed by Haemophilus ducreyi are a novel class of pili. J Bacteriol. 1996;178:808-16 pubmed
    b>Haemophilus ducreyi synthesizes fine, tangled pili composed predominantly of a protein whose apparent molecular weight is 24,000 (24K). A hybridoma, 2D8, produced a monoclonal antibody (MAb) that bound to a 24K protein in H...
  37. Mount K, Townsend C, Rinker S, Gu X, Fortney K, Zwickl B, et al. Haemophilus ducreyi SapA contributes to cathelicidin resistance and virulence in humans. Infect Immun. 2010;78:1176-84 pubmed publisher
    b>Haemophilus ducreyi is an extracellular pathogen of human epithelial surfaces that resists human antimicrobial peptides (APs)...
  38. Lewis D, Stevens M, Latimer J, Ward C, Deng K, Blick R, et al. Characterization of Haemophilus ducreyi cdtA, cdtB, and cdtC mutants in in vitro and in vivo systems. Infect Immun. 2001;69:5626-34 pubmed
    b>Haemophilus ducreyi expresses a soluble cytolethal distending toxin (CDT) that is encoded by the cdtABC gene cluster and can be detected in culture supernatant fluid by its ability to kill HeLa cells. The cdtA, cdtB, and cdtC genes of H...
  39. Labandeira Rey M, Brautigam C, Hansen E. Characterization of the CpxRA regulon in Haemophilus ducreyi. Infect Immun. 2010;78:4779-91 pubmed publisher
    The Haemophilus ducreyi 35000HP genome encodes a homolog of the CpxRA two-component cell envelope stress response system originally characterized in Escherichia coli...
  40. Elkins C, Morrow K, Olsen B. Serum resistance in Haemophilus ducreyi requires outer membrane protein DsrA. Infect Immun. 2000;68:1608-19 pubmed
    b>Haemophilus ducreyi is resistant to killing by normal serum antibody and complement. We discovered an H...
  41. Rinker S, Trombley M, Gu X, Fortney K, Bauer M. Deletion of mtrC in Haemophilus ducreyi increases sensitivity to human antimicrobial peptides and activates the CpxRA regulon. Infect Immun. 2011;79:2324-34 pubmed publisher
    b>Haemophilus ducreyi resists killing by antimicrobial peptides encountered during human infection, including cathelicidin LL-37, ?-defensins, and ?-defensins...
  42. Bozja J, Yi K, Shafer W, Stojiljkovic I. Porphyrin-based compounds exert antibacterial action against the sexually transmitted pathogens Neisseria gonorrhoeae and Haemophilus ducreyi. Int J Antimicrob Agents. 2004;24:578-84 pubmed
    ..potent bactericidal action in vitro against the sexually transmitted pathogens Neisseria gonorrhoeae and Haemophilus ducreyi. nMP and MP did not show bactericidal activity against five species of lactobacilli...
  43. Bauer M, Fortney K, Harrison A, Janowicz D, Munson R, Spinola S. Identification of Haemophilus ducreyi genes expressed during human infection. Microbiology. 2008;154:1152-60 pubmed publisher
    To identify Haemophilus ducreyi transcripts that are expressed during human infection, we used selective capture of transcribed sequences (SCOTS) with RNA isolated from pustules obtained from three volunteers infected with H...
  44. Leduc I, Banks K, Fortney K, Patterson K, Billings S, Katz B, et al. Evaluation of the repertoire of the TonB-dependent receptors of Haemophilus ducreyi for their role in virulence in humans. J Infect Dis. 2008;197:1103-9 pubmed publisher
    b>Haemophilus ducreyi contains 3 TonB-dependent receptors: the hemoglobin receptor HgbA, which is required for virulence in humans; the heme receptor TdhA; and an uncharacterized conserved hypothetical protein TdX (HD0646)...
  45. Ward C, Latimer J, Nika J, Vakevainen M, Mock J, Deng K, et al. Mutations in the lspA1 and lspA2 genes of Haemophilus ducreyi affect the virulence of this pathogen in an animal model system. Infect Immun. 2003;71:2478-86 pubmed
    b>Haemophilus ducreyi 35000HP contains two genes, lspA1 and lspA2, whose predicted protein products have molecular weights of 456,000 and 543,000, respectively (C. K. Ward, S. R. Lumbley, J. L. Latimer, L. D. Cope, and E. J. Hansen, J...
  46. Li L, Sharipo A, Chaves Olarte E, Masucci M, Levitsky V, Thelestam M, et al. The Haemophilus ducreyi cytolethal distending toxin activates sensors of DNA damage and repair complexes in proliferating and non-proliferating cells. Cell Microbiol. 2002;4:87-99 pubmed
    ..We demonstrate that the Haemophilus ducreyi CDT (HdCDT) induces phosphorylation of the histone H2AX as early as 1 h after intoxication and re-..
  47. Mount K, Townsend C, Bauer M. Haemophilus ducreyi is resistant to human antimicrobial peptides. Antimicrob Agents Chemother. 2007;51:3391-3 pubmed
    We examined the susceptibility of Haemophilus ducreyi to antimicrobial peptides likely to be encountered in vivo during human infection. H...
  48. Totten P, Kuypers J, Chen C, Alfa M, Parsons L, Dutro S, et al. Etiology of genital ulcer disease in Dakar, Senegal, and comparison of PCR and serologic assays for detection of Haemophilus ducreyi. J Clin Microbiol. 2000;38:268-73 pubmed
    ..serological tests (adsorption enzyme immunoassay [EIA] and lipooligosaccharide [LOS] EIA) to detect current Haemophilus ducreyi infection. We found that in this population, H. ducreyi, T...
  49. Filiatrault M, Munson R, Campagnari A. Genetic analysis of a pyocin-resistant lipooligosaccharide (LOS) mutant of Haemophilus ducreyi: restoration of full-length LOS restores pyocin sensitivity. J Bacteriol. 2001;183:5756-61 pubmed
    DNA sequence and Southern blot analyses were used to determine the genetic defect of a Haemophilus ducreyi pyocin-resistant lipooligosaccharide (LOS) mutant, HD35000R...
  50. Post D, Mungur R, Gibson B, Munson R. Identification of a novel sialic acid transporter in Haemophilus ducreyi. Infect Immun. 2005;73:6727-35 pubmed
    b>Haemophilus ducreyi, the causative agent of chancroid, produces a lipooligosaccharide (LOS) which terminates in N-acetyllactosamine...
  51. Svensson L, Henning P, Lagergard T. The cytolethal distending toxin of Haemophilus ducreyi inhibits endothelial cell proliferation. Infect Immun. 2002;70:2665-9 pubmed
    b>Haemophilus ducreyi, the etiologic agent of the sexually transmitted disease chancroid, produces a cytolethal distending toxin (HdCDT) that inhibits mammalian cell proliferation...
  52. Nika J, Latimer J, Ward C, Blick R, Wagner N, Cope L, et al. Haemophilus ducreyi requires the flp gene cluster for microcolony formation in vitro. Infect Immun. 2002;70:2965-75 pubmed
    b>Haemophilus ducreyi, the etiologic agent of chancroid, has been shown to form microcolonies when cultured in the presence of human foreskin fibroblasts. We identified a 15-gene cluster in H...
  53. Fusco W, Elkins C, Leduc I. Trimeric autotransporter DsrA is a major mediator of fibrinogen binding in Haemophilus ducreyi. Infect Immun. 2013;81:4443-52 pubmed publisher
    b>Haemophilus ducreyi is the etiologic agent of the sexually transmitted genital ulcer disease chancroid. In both natural and experimental chancroid, H. ducreyi colocalizes with fibrin at the base of the ulcer...
  54. Orle K, Gates C, Martin D, Body B, Weiss J. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. J Clin Microbiol. 1996;34:49-54 pubmed
    ..assay with colorimetric detection was devised for the simultaneous amplification of DNA targets from Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus (HSV) types 1 and 2...
  55. Ward C, Lumbley S, Latimer J, Cope L, Hansen E. Haemophilus ducreyi secretes a filamentous hemagglutinin-like protein. J Bacteriol. 1998;180:6013-22 pubmed
    We have identified two extremely large open reading frames (ORFs) in Haemophilus ducreyi 35000, lspA1 and lspA2, each of which encodes a predicted protein product whose N-terminal half is approximately 43% similar to the N-terminal half ..
  56. Ahmed H, Svensson L, Cope L, Latimer J, Hansen E, Ahlman K, et al. Prevalence of cdtABC genes encoding cytolethal distending toxin among Haemophilus ducreyi and Actinobacillus actinomycetemcomitans strains. J Med Microbiol. 2001;50:860-4 pubmed
    ..the presence of the three cdtABC genes responsible for production of cytolethal distending toxin (CDT) in Haemophilus ducreyi and Actinobacillus actinomycetemcomitans strains. Of 100 H...
  57. Leduc I, Olsen B, Elkins C. Localization of the domains of the Haemophilus ducreyi trimeric autotransporter DsrA involved in serum resistance and binding to the extracellular matrix proteins fibronectin and vitronectin. Infect Immun. 2009;77:657-66 pubmed publisher
    ..In the gram-negative mucosal pathogen Haemophilus ducreyi, serum resistance is mediated primarily by the trimeric autotransporter DsrA...
  58. Negari S, Sulpher J, Pacello F, Ingrey K, Battistoni A, Lee B. A role for Haemophilus ducreyi Cu,ZnSOD in resistance to heme toxicity. Biometals. 2008;21:249-58 pubmed
    The Cu,Zn superoxide dismutase (Cu,ZnSOD) from Haemophilus ducreyi is the only enzyme of this class which binds a heme molecule at its dimer interface...
  59. Banks K, Humphreys T, Li W, Katz B, Wilkes D, Spinola S. Haemophilus ducreyi partially activates human myeloid dendritic cells. Infect Immun. 2007;75:5678-85 pubmed
    Dendritic cells (DC) orchestrate innate and adaptive immune responses to bacteria. How Haemophilus ducreyi, which causes genital ulcers and regional lymphadenitis, interacts with DC is unknown. H...
  60. Pacello F, Langford P, Kroll J, Indiani C, Smulevich G, Desideri A, et al. A novel heme protein, the Cu,Zn-superoxide dismutase from Haemophilus ducreyi. J Biol Chem. 2001;276:30326-34 pubmed
    b>Haemophilus ducreyi, the causative agent of the genital ulcerative disease known as chancroid, is unable to synthesize heme, which it acquires from humans, its only known host...
  61. Li W, Katz B, Spinola S. Haemophilus ducreyi lipooligosaccharides induce expression of the immunosuppressive enzyme indoleamine 2,3-dioxygenase via type I interferons and tumor necrosis factor alpha in human dendritic cells. Infect Immun. 2011;79:3338-47 pubmed publisher
    b>Haemophilus ducreyi causes chancroid, a genital ulcer disease. In human inoculation experiments, most volunteers fail to clear the bacteria despite the infiltration of innate and adaptive immune cells to the infected sites...
  62. Li Y, Sun M, Huang S, Yu H, Chokhawala H, Thon V, et al. The Hd0053 gene of Haemophilus ducreyi encodes an alpha2,3-sialyltransferase. Biochem Biophys Res Commun. 2007;361:555-60 pubmed
    b>Haemophilus ducreyi is a Gram-negative bacterium that causes chancroid, a sexually transmitted genital ulcer disease. Different lipooligosaccharide (LOS) structures have been identified from H...
  63. Gangaiah D, Zhang X, Fortney K, Baker B, Liu Y, Munson R, et al. Activation of CpxRA in Haemophilus ducreyi primarily inhibits the expression of its targets, including major virulence determinants. J Bacteriol. 2013;195:3486-502 pubmed publisher
    b>Haemophilus ducreyi causes chancroid, a genital ulcer disease that facilitates the transmission of human immunodeficiency virus type 1. In humans, H. ducreyi is surrounded by phagocytes and must adapt to a hostile environment to survive...
  64. Lagergard T, Lundqvist A, Wising C, Gabrielsson V, Ahlman K. Formaldehyde treatment increases the immunogenicity and decreases the toxicity of Haemophilus ducreyi cytolethal distending toxin. Vaccine. 2007;25:3606-14 pubmed
    b>Haemophilus ducreyi cytolethal distending toxin (HdCDT) is a tripartite AB toxin, which causes DNA damage in affected cells...
  65. Patterson K, Olsen B, Thomas C, Norn D, Tam M, Elkins C. Development of a rapid immunodiagnostic test for Haemophilus ducreyi. J Clin Microbiol. 2002;40:3694-702 pubmed
    b>Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted disease that increases the rate of transmission of human immunodeficiency virus...
  66. Labandeira Rey M, Mock J, Hansen E. Regulation of expression of the Haemophilus ducreyi LspB and LspA2 proteins by CpxR. Infect Immun. 2009;77:3402-11 pubmed publisher
    The LspA1, LspA2, and LspB proteins of Haemophilus ducreyi comprise a two-partner secretion system that has been shown to be necessary for H. ducreyi to inhibit phagocytosis by immune cells in vitro...
  67. Li W, Janowicz D, Fortney K, Katz B, Spinola S. Mechanism of human natural killer cell activation by Haemophilus ducreyi. J Infect Dis. 2009;200:590-8 pubmed publisher
    The role of natural killer (NK) cells in the host response to Haemophilus ducreyi infection is unclear...
  68. Ahmed H, Johansson C, Svensson L, Ahlman K, Verdrengh M, Lagergard T. In vitro and in vivo interactions of Haemophilus ducreyi with host phagocytes. Infect Immun. 2002;70:899-908 pubmed
    We investigated the phagocytosis of Haemophilus ducreyi both in vitro and in vivo. Human granulocyte and monocyte phagocytosis of opsonized and nonopsonized, fluorescence-labeled H. ducreyi was assessed by flow cytometry...
  69. Frisan T, Cortes Bratti X, Chaves Olarte E, Stenerlow B, Thelestam M. The Haemophilus ducreyi cytolethal distending toxin induces DNA double-strand breaks and promotes ATM-dependent activation of RhoA. Cell Microbiol. 2003;5:695-707 pubmed
    ..We provide direct evidence that natural intoxication of cells with the Haemophilus ducreyi CDT (HdCDT) holotoxin induces DNA double-strand breaks similarly to ionizing radiation...
  70. Post D, Gibson B. Proposed second class of Haemophilus ducreyi strains show altered protein and lipooligosaccharide profiles. Proteomics. 2007;7:3131-42 pubmed
    b>Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted genital ulcer disease. Previously we have shown that the protein profiles and lipooligosaccharide (LOS) structures from various strains of H...
  71. Li W, Katz B, Spinola S. Haemophilus ducreyi-induced interleukin-10 promotes a mixed M1 and M2 activation program in human macrophages. Infect Immun. 2012;80:4426-34 pubmed publisher
    ..To study the role of macrophage polarization during Haemophilus ducreyi infection, we analyzed a panel of macrophage surface markers in skin biopsy specimens of pustules obtained ..