thermus

Summary

Summary: Gram-negative aerobic rods found in warm water (40-79 degrees C) such as hot springs, hot water tanks, and thermally polluted rivers.

Top Publications

  1. Ohtsu N, Motoshima H, Goto K, Tsukasaki F, Matsuzawa H. Thermostable beta-galactosidase from an extreme thermophile, Thermus sp. A4: enzyme purification and characterization, and gene cloning and sequencing. Biosci Biotechnol Biochem. 1998;62:1539-45 pubmed
    We purified and characterized a thermophilic beta-galactosidase from Thermus sp. A4 isolated from the Atagawa hot spring (Shizuoka, Japan)...
  2. Karlin S, Mrazek J. Predicted highly expressed and putative alien genes of Deinococcus radiodurans and implications for resistance to ionizing radiation damage. Proc Natl Acad Sci U S A. 2001;98:5240-5 pubmed
    ..The top PHX gene of DEIRA is the multifunctional tricarboxylic acid (TCA) gene aconitase, which, apart from its role in respiration, also alerts the cell to oxidative damage. ..
  3. Fedorov R, Witte G, Urbanke C, Manstein D, Curth U. 3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins. Nucleic Acids Res. 2006;34:6708-17 pubmed
    In contrast to the majority of tetrameric SSB proteins, the recently discovered SSB proteins from the Thermus/Deinoccus group form dimers...
  4. Borukhov S, Severinov K. Role of the RNA polymerase sigma subunit in transcription initiation. Res Microbiol. 2002;153:557-62 pubmed
  5. Yuzenkova Y, Tadigotla V, Severinov K, Zenkin N. A new basal promoter element recognized by RNA polymerase core enzyme. EMBO J. 2011;30:3766-75 pubmed publisher
    ..Finally, our data suggest that sequence-dependent interactions of the ?' zipper with DNA can contribute to promoter-proximal ?-dependent RNAP pausing, a recently recognized important step of transcription control. ..
  6. Kjeldgaard M, Nissen P, Thirup S, Nyborg J. The crystal structure of elongation factor EF-Tu from Thermus aquaticus in the GTP conformation. Structure. 1993;1:35-50 pubmed
    ..During this interaction, GTP is hydrolyzed, and EF-Tu.GDP is ejected. The crystal structure of EF-Tu from Thermus aquaticus, complexed to the GTP analogue GDPNP, has been determined at 2...
  7. Antony E, Hingorani M. Asymmetric ATP binding and hydrolysis activity of the Thermus aquaticus MutS dimer is key to modulation of its interactions with mismatched DNA. Biochemistry. 2004;43:13115-28 pubmed
    ..We have found the Thermus aquaticus MutS protein amenable to detailed investigation of the nature and role of this asymmetry...
  8. Fuciños P, Dominguez A, Sanroman M, Longo M, Rúa M, Pastrana L. Production of thermostable lipolytic activity by thermus species. Biotechnol Prog. 2005;21:1198-205 pubmed
    ..screening for intra- and extracellular lipolytic activity was performed in submerged cultures of four Thermus strains using two different media (named T or D medium)...
  9. Yang Y, LiCata V. Interactions of replication versus repair DNA substrates with the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus. Biophys Chem. 2011;159:188-93 pubmed publisher
    ..The DNA binding preferences of Klenow and Klentaq DNA polymerases, from Escherichia coli and Thermus aquaticus respectively, have been studied using a fluorescence competition binding assay...

More Information

Publications127 found, 100 shown here

  1. Bailey S, Eliason W, Steitz T. The crystal structure of the Thermus aquaticus DnaB helicase monomer. Nucleic Acids Res. 2007;35:4728-36 pubmed
    ..We have solved the crystal structure of the full-length Thermus aquaticus DnaB monomer, or possibly dimer, at 2.9 A resolution. DnaB is a highly flexible two domain protein...
  2. Lapierre P, Shial R, Gogarten J. Distribution of F- and A/V-type ATPases in Thermus scotoductus and other closely related species. Syst Appl Microbiol. 2006;29:15-23 pubmed
    The presence of an A/V-type ATPase in different Thermus species and in the deeper branching species Meiothermus ruber and Deinococcus radiodurans suggests that the presence of the archaeal-type ATPase is a primitive character of the ..
  3. Mekler V, Minakhin L, Severinov K. A critical role of downstream RNA polymerase-promoter interactions in the formation of initiation complex. J Biol Chem. 2011;286:22600-8 pubmed publisher
  4. Lamour V, Hogan B, Erie D, Darst S. Crystal structure of Thermus aquaticus Gfh1, a Gre-factor paralog that inhibits rather than stimulates transcript cleavage. J Mol Biol. 2006;356:179-88 pubmed
    ..A GreA paralog, Gfh1, present in Thermus aquaticus and Thermus thermophilus, has the opposite effect on elongation complexes, inhibiting rather than ..
  5. Ramirez U, Minasov G, Focia P, Stroud R, Walter P, Kuhn P, et al. Structural basis for mobility in the 1.1 A crystal structure of the NG domain of Thermus aquaticus Ffh. J Mol Biol. 2002;320:783-99 pubmed
    ..Here, we report the structure of the apo NG GTPase of Ffh from Thermus aquaticus refined at 1.10 A resolution...
  6. Lee J, Im Y, Bae J, Kim D, Kim M, Kang G, et al. Crystal structure of Thermus caldophilus phosphoglycerate kinase in the open conformation. Biochem Biophys Res Commun. 2006;350:1044-9 pubmed publisher
    ..Here we present the 1.8A crystal structure of unliganded PGK from Thermus caldophilus (Tca)...
  7. Pazhang M, Khajeh K, Asghari S, Falahati H, Naderi Manesh H. Cloning, expression, and characterization of a novel methylglyoxal synthase from Thermus sp. strain GH5. Appl Biochem Biotechnol. 2010;162:1519-28 pubmed publisher
    A gene encoding methylglyoxal synthase from Thermus sp. GH5 (TMGS) was cloned, sequenced, overexpressed, and purified by Q-Sepharose...
  8. Jung J, Jung T, Seo D, Yoon S, Choi H, Park B, et al. Structural and functional analysis of substrate recognition by the 250s loop in amylomaltase from Thermus brockianus. Proteins. 2011;79:633-44 pubmed publisher
    ..In this study, we determined the crystal structure of amylomaltase from Thermus brockianus at a resolution of 2...
  9. Lawyer F, Stoffel S, Saiki R, Chang S, Landre P, Abramson R, et al. High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. PCR Methods Appl. 1993;2:275-87 pubmed
    The Thermus aquaticus DNA polymerase I (Taq Pol I) gene was cloned into a plasmid expression vector that utilizes the strong bacteriophage lambda PL promoter. A truncated form of Taq Pol I was also constructed...
  10. Jedrzejczak R, Dauter M, Dauter Z, Olszewski M, Długołecka A, Kur J. Structure of the single-stranded DNA-binding protein SSB from Thermus aquaticus. Acta Crystallogr D Biol Crystallogr. 2006;62:1407-12 pubmed
    The crystal structure of the single-stranded DNA-binding protein from Thermus aquaticus has been solved and refined at 1.85 A resolution...
  11. Darbon E, Ito K, Huang H, Yoshimoto T, Poncet S, Deutscher J. Glycerol transport and phosphoenolpyruvate-dependent enzyme I- and HPr-catalysed phosphorylation of glycerol kinase in Thermus flavus. Microbiology. 1999;145 ( Pt 11):3205-12 pubmed
    The genes glpK and glpF, encoding glycerol kinase and the glycerol facilitator of Thermus flavus, a member of the Thermus/Deinococcus group, have recently been identified...
  12. Newby Z, Lau E, Bruice T. A theoretical examination of the factors controlling the catalytic efficiency of the DNA-(adenine-N6)-methyltransferase from Thermus aquaticus. Proc Natl Acad Sci U S A. 2002;99:7922-7 pubmed
    ..calculations have been carried out to more fully understand the factors controlling the catalytic activity of the Thermus aquaticus DNA methyltransferase (MTaqI) in the N-methylation at the N(6) of an adenine nucleobase...
  13. Albuquerque L, Simões C, Nobre M, Pino N, Battista J, Silva M, et al. Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov. FEMS Microbiol Lett. 2005;247:161-9 pubmed
    Two isolates, belonging to a new species of a novel genus of the Phylum "Deinococcus/Thermus ", were recovered from hot spring runoffs on the Island of São Miguel in the Azores...
  14. Ruan L, Xu X. Sequence analysis and characterizations of two novel plasmids isolated from Thermus sp. 4C. Plasmid. 2007;58:84-7 pubmed
    Two novel plasmids, named pS4C and pL4C, were isolated from the thermophilic bacterium Thermus sp. 4C. The pS4C with a length of 5015bp and 58.25% of G+C content, contains 9 putative open reading frames (ORFs)...
  15. Gibbs M, Reeves R, Mandelman D, Mi Q, Lee J, Bergquist P. Molecular diversity and catalytic activity of Thermus DNA polymerases. Extremophiles. 2009;13:817-26 pubmed publisher
    b>Thermus aquaticus DNA polymerase (Taq polymerase) made the polymerase chain reaction feasible and led to a paradigm shift in genomic analysis...
  16. Nishiyama M, Matsubara N, Yamamoto K, Iijima S, Uozumi T, Beppu T. Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity. J Biol Chem. 1986;261:14178-83 pubmed
    The nucleotide sequence of the malate dehydrogenase (mdh) gene from a thermophilic bacterium, Thermus flavus, was determined...
  17. Vichier Guerre S, Ferris S, Auberger N, Mahiddine K, Jestin J. A population of thermostable reverse transcriptases evolved from Thermus aquaticus DNA polymerase I by phage display. Angew Chem Int Ed Engl. 2006;45:6133-7 pubmed
  18. Noah J, Shapkina T, Wollenzien P. UV-induced crosslinks in the 16S rRNAs of Escherichia coli, Bacillus subtilis and Thermus aquaticus and their implications for ribosome structure and photochemistry. Nucleic Acids Res. 2000;28:3785-92 pubmed
    ..Crosslinking patterns in two other organisms, Bacillus subtilis and Thermus aquaticus, were investigated to determine if the number and location of crosslinks in E...
  19. Alarico S, Empadinhas N, Sim es C, Silva Z, Henne A, Mingote A, et al. Distribution of genes for synthesis of trehalose and Mannosylglycerate in Thermus spp. and direct correlation of these genes with halotolerance. Appl Environ Microbiol. 2005;71:2460-6 pubmed publisher
    ..the presence of genes leading to the synthesis of trehalose and mannosylglycerate (MG) in 17 strains of the genus Thermus with the ability of the strains to grow and accumulate these compatible solutes in a defined medium containing ..
  20. Opperman D, Sewell B, Litthauer D, Isupov M, Littlechild J, van Heerden E. Crystal structure of a thermostable old yellow enzyme from Thermus scotoductus SA-01. Biochem Biophys Res Commun. 2010;393:426-31 pubmed publisher
    Recent characterization of the chromate reductase (CrS) from the thermophile Thermus scotoductus SA-01 revealed this enzyme to be related to the Old Yellow Enzyme (OYE) family...
  21. Lin L, Hong W, Ji X, Han J, Huang L, Wei Y. Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China. J Basic Microbiol. 2010;50:452-6 pubmed publisher
    b>Thermus strains are regarded as models to investigate the mechanism of thermostability of thermophiles, and phages from Thermus are particularly interesting because of their way to regulate gene expression...
  22. Skirnisdottir S, Hreggvidsson G, Holst O, Kristjansson J. Isolation and characterization of a mixotrophic sulfur-oxidizing Thermus scotoductus. Extremophiles. 2001;5:45-51 pubmed
    ..The new isolate belonged to Thermus scotoductus because the small subunit (SSU) rRNA gene sequence analysis showed 98...
  23. Kim J, Kim Y, Roh H, Kim H, Cha J, Park K, et al. Production of tagatose by a recombinant thermostable L-arabinose isomerase from Thermus sp. IM6501. Biotechnol Lett. 2003;25:963-7 pubmed
    A gene (thaI) corresponding to L-arabinose isomerase from Thermus strain IM6501 was cloned by PCR. It comprised 1488 nucleotides and encoded a polypeptide of 496 residues with a predicted molecular weight of 56019 Da...
  24. Kim Y, Choi J, Kim J, Park C, Kim J, Cha H, et al. Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance. Appl Environ Microbiol. 2003;69:4866-74 pubmed
    The thermostability of maltogenic amylase from Thermus sp. strain IM6501 (ThMA) was improved greatly by random mutagenesis using DNA shuffling...
  25. Skowron P, Vitkute J, Ramanauskaite D, Mitkaite G, Jezewska Frackowiak J, Zebrowska J, et al. Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus. BMC Mol Biol. 2013;14:17 pubmed publisher
    ..family includes six thermostable enzymes: TaqII, Tth111II, TthHB27I, TspGWI, TspDTI, TsoI, isolated from various Thermus sp...
  26. Housby J, Thorbjarnardottir S, Jónsson Z, Southern E. Optimised ligation of oligonucleotides by thermal ligases: comparison of Thermus scotoductus and Rhodothermus marinus DNA ligases to other thermophilic ligases. Nucleic Acids Res. 2000;28:E10 pubmed
    We describe the characterisation of four thermo-stable NAD(+)-dependent DNA ligases, from Thermus thermophilus (Tth), Thermus scotoductus (Ts), Rhodothermus marinus (Rm) and Thermus aquaticus (Taq), by an assay which measures ligation ..
  27. Zylicz Stachula A, Harasimowicz Slowińska R, Sobolewski I, Skowron P. TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'. Nucleic Acids Res. 2002;30:e33 pubmed
    A novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW...
  28. Datta K, LiCata V. Salt dependence of DNA binding by Thermus aquaticus and Escherichia coli DNA polymerases. J Biol Chem. 2003;278:5694-701 pubmed
    DNA binding properties of the Type 1 DNA polymerases from Thermus aquaticus (Taq, Klentaq) and Escherichia coli (Klenow) have been examined as a function of [KCl] and [MgCl(2)]...
  29. Fujii K, Minagawa H, Terada Y, Takaha T, Kuriki T, Shimada J, et al. Use of random and saturation mutageneses to improve the properties of Thermus aquaticus amylomaltase for efficient production of cycloamyloses. Appl Environ Microbiol. 2005;71:5823-7 pubmed
    Amylomaltase from Thermus aquaticus catalyzes intramolecular transglycosylation of alpha-1,4 glucans to produce cyclic alpha-1,4 glucans (cycloamyloses) with degrees of polymerization of 22 and higher...
  30. Gounder K, Brzuszkiewicz E, Liesegang H, Wollherr A, Daniel R, Gottschalk G, et al. Sequence of the hyperplastic genome of the naturally competent Thermus scotoductus SA-01. BMC Genomics. 2011;12:577 pubmed publisher
    Many strains of Thermus have been isolated from hot environments around the world. Thermus scotoductus SA-01 was isolated from fissure water collected 3.2 km below surface in a South African gold mine...
  31. Mekler V, Minakhin L, Kuznedelov K, Mukhamedyarov D, Severinov K. RNA polymerase-promoter interactions determining different stability of the Escherichia coli and Thermus aquaticus transcription initiation complexes. Nucleic Acids Res. 2012;40:11352-62 pubmed publisher
    ..The molecular basis for this diversity is unclear. Promoter complexes formed by RNAP from Thermus aquaticus (Taq) are considerably less stable than Escherichia coli RNAP promoter complexes, particularly at ..
  32. Ma W, Kaiser M, Lyamicheva N, Schaefer J, Allawi H, Takova T, et al. RNA template-dependent 5' nuclease activity of Thermus aquaticus and Thermus thermophilus DNA polymerases. J Biol Chem. 2000;275:24693-700 pubmed
    ..Analysis of the activity of chimeric enzymes between the DNA polymerases from Thermus aquaticus (TaqPol) and Thermus thermophilus (TthPol) reveals two regions, in the "thumb" and in the &..
  33. Dominguez A, Sanroman A, Fuciños P, Rua M, Pastrana L, Longo M. Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp. Biotechnol Lett. 2004;26:705-8 pubmed
    Four Thermus strains produced lipolytic activity when grown in liquid medium for 30 h at 70 degrees C...
  34. Möller C, van Heerden E. Isolation of a soluble and membrane-associated Fe(III) reductase from the thermophile, Thermus scotoductus (SA-01). FEMS Microbiol Lett. 2006;265:237-43 pubmed
    The Fe(III) reductase activity was studied in the South African Fe(III)-reducing bacterium, Thermus scotoductus (SA-01)...
  35. Vitkute J, Maneliene Z, Janulaitis A. Two new thermostable type II restriction endonucleases from Thermus aquaticus: TatI and TauI, which recognize the novel nucleotide sequences 5'-W (downward arrow)GTACW-3' and 5'-GCSG (downward arrow)C-3' respectively. FEMS Microbiol Lett. 2001;204:253-7 pubmed
    One hundred and forty isolates of thermophilic bacteria from the genus Thermus were screened for the presence of restriction endonuclease activity...
  36. Kuznedelov K, Korzheva N, Mustaev A, Severinov K. Structure-based analysis of RNA polymerase function: the largest subunit's rudder contributes critically to elongation complex stability and is not involved in the maintenance of RNA-DNA hybrid length. EMBO J. 2002;21:1369-78 pubmed
    ..Our results suggest that the rudder makes critical contributions to elongation complex stability through direct interactions with the nascent RNA. ..
  37. Zhang X, Ying Y, Ye Y, Xu X, Zhu X, Wu M. Thermus arciformis sp. nov., a thermophilic species from a geothermal area. Int J Syst Evol Microbiol. 2010;60:834-9 pubmed publisher
    ..levels of DNA-DNA relatedness together indicated that the new isolates represented a novel species of the genus Thermus with closest affinity to Thermus aquaticus, Thermus igniterrae and Thermus thermophilus...
  38. Nissen P, Kjeldgaard M, Thirup S, Polekhina G, Reshetnikova L, Clark B, et al. Crystal structure of the ternary complex of Phe-tRNAPhe, EF-Tu, and a GTP analog. Science. 1995;270:1464-72 pubmed
    The structure of the ternary complex consisting of yeast phenylalanyl-transfer RNA (Phe-tRNAPhe), Thermus aquaticus elongation factor Tu (EF-Tu), and the guanosine triphosphate (GTP) analog GDPNP was determined by x-ray crystallography ..
  39. Toyoda T, Tin O, Ito K, Fujiwara T, Kumasaka T, Yamamoto M, et al. Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch. RNA. 2000;6:1432-44 pubmed
    ..The crystal structure of Thermus thermophilus RRF was determined at 2.6 A resolution...
  40. Dabrowski S, Olszewski M, Piatek R, Brillowska Dabrowska A, Konopa G, Kur J. Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus aquaticus - new arrangement of binding domains. Microbiology. 2002;148:3307-15 pubmed
    ..This paper reports the identification and characterization of the SSB-like proteins of the thermophilic bacteria Thermus thermophilus and Thermus aquaticus...
  41. Opperman D, van Heerden E. A membrane-associated protein with Cr(VI)-reducing activity from Thermus scotoductus SA-01. FEMS Microbiol Lett. 2008;280:210-8 pubmed publisher
    A membrane-associated chromate reductase from Thermus scotoductus SA-01 has been purified to apparent homogeneity and shown to couple the reduction of Cr(VI) to NAD(P)H oxidation, with a preference towards NADH...
  42. Tessmer I, Yang Y, Zhai J, Du C, Hsieh P, Hingorani M, et al. Mechanism of MutS searching for DNA mismatches and signaling repair. J Biol Chem. 2008;283:36646-54 pubmed publisher
    ..These results provide a structural explanation for the mechanism by which MutS searches for and recognizes mismatches and for the observed phenotypes of mutants with substitutions in the Phe-Xaa-Glu motif. ..
  43. Cao W, Lu J, Barany F. Nucleotide sequences and gene organization of TaqI endonuclease isoschizomers from Thermus sp. SM32 and Thermus filiformis Tok6A1. Gene. 1997;197:205-14 pubmed
    ..Accordingly, these isoschizomers were arranged into four geographical groups, i.e., USA as represented by Thermus aquaticus YT1, Japan by Thermus thermophilus HB8, New Zealand by Thermus filiformis Tok6A1, Portugal by Thermus ..
  44. Zylicz Stachula A, Zolnierkiewicz O, Lubys A, Ramanauskaite D, Mitkaite G, Bujnicki J, et al. Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities. BMC Mol Biol. 2012;13:13 pubmed publisher
    We previously defined a family of restriction endonucleases (REases) from Thermus sp...
  45. Polekhina G, Thirup S, Kjeldgaard M, Nissen P, Lippmann C, Nyborg J. Helix unwinding in the effector region of elongation factor EF-Tu-GDP. Structure. 1996;4:1141-51 pubmed
    ..The crystal structures of intact EF-Tu-GDP from Thermus aquaticus and Escherichia coli have been determined at resolutions of 2.7 A and 3.8 A, respectively...
  46. Murakami K, Masuda S, Campbell E, Muzzin O, Darst S. Structural basis of transcription initiation: an RNA polymerase holoenzyme-DNA complex. Science. 2002;296:1285-90 pubmed publisher
    The crystal structure of Thermus aquaticus RNA polymerase holoenzyme (alpha2betabeta'omegasigmaA) complexed with a fork-junction promoter DNA fragment has been determined by fitting high-resolution x-ray structures of individual ..
  47. Saiki R, Gelfand D, Stoffel S, Scharf S, Higuchi R, Horn G, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988;239:487-91 pubmed
    ..The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher ..
  48. Murakami K, Darst S. Bacterial RNA polymerases: the wholo story. Curr Opin Struct Biol. 2003;13:31-9 pubmed
    ..Comparisons with structural analyses of evolutionarily unrelated RNA polymerases reveal unexpected general features of the initiation process. ..
  49. Opperman D, Van Heerden E. Aerobic Cr(VI) reduction by Thermus scotoductus strain SA-01. J Appl Microbiol. 2007;103:1907-13 pubmed
    To evaluate Thermus scotoductus SA-01's ability to reduce Cr(VI) aerobically. T. scotoductus SA-01 is able to reduce Cr(VI) aerobically when grown in a complex organic medium containing Cr(VI) concentrations up to 0.5 mmol l(-1)...
  50. Stallings W, Pattridge K, Strong R, Ludwig M. The structure of manganese superoxide dismutase from Thermus thermophilus HB8 at 2.4-A resolution. J Biol Chem. 1985;260:16424-32 pubmed
    An atomic model of tetrameric manganese superoxide dismutase from Thermus thermophilus HB8 has been built into an electron density map at 2...
  51. Campbell E, Muzzin O, Chlenov M, Sun J, Olson C, Weinman O, et al. Structure of the bacterial RNA polymerase promoter specificity sigma subunit. Mol Cell. 2002;9:527-39 pubmed
    The sigma subunit is the key regulator of bacterial transcription. Proteolysis of Thermus aquaticus sigma(A), which occurred in situ during crystallization, reveals three domains, sigma(2), sigma(3), and sigma(4), connected by flexible ..
  52. Opperman D, Piater L, van Heerden E. A novel chromate reductase from Thermus scotoductus SA-01 related to old yellow enzyme. J Bacteriol. 2008;190:3076-82 pubmed publisher
    Bacteria can reduce toxic and carcinogenic Cr(VI) to insoluble and less toxic Cr(III). Thermus scotoductus SA-01, a South African gold mine isolate, has been shown to be able to reduce a variety of metals, including Cr(VI)...
  53. Focia P, Shepotinovskaya I, Seidler J, Freymann D. Heterodimeric GTPase core of the SRP targeting complex. Science. 2004;303:373-7 pubmed
    ..We propose that the complex represents a molecular "latch" and that its disengagement is regulated by completion of assembly of the GTPase active site. ..
  54. Yu M, Slater M, Ackermann H. Isolation and characterization of Thermus bacteriophages. Arch Virol. 2006;151:663-79 pubmed
    ..This is the first time that tectiviruses and filamentous phages are reported for bacteria of the Thermus-Deinococcus phylum...
  55. Williams R, Smith K, Welch S, Micallef J. Thermus oshimai sp. nov., isolated from hot springs in Portugal, Iceland, and the Azores, and comment on the concept of a limited geographical distribution of Thermus species. Int J Syst Bacteriol. 1996;46:403-8 pubmed
    ..thermophilic, gram-negative bacteria that were isolated from hot springs in Portugal and were identified as Thermus strains and placed in phenetic groups on the basis of their phenotypic characteristics...
  56. Schroeder L, deHaseth P. Mechanistic differences in promoter DNA melting by Thermus aquaticus and Escherichia coli RNA polymerases. J Biol Chem. 2005;280:17422-9 pubmed
    ..complexes at promoters was compared for RNA polymerases from the mesophile Escherichia coli and the thermophile Thermus aquaticus...
  57. Du X, P ne J. Identification, cloning and expression of p25, an AT-rich DNA-binding protein from the extreme thermophile, Thermus aquaticus YT-1. Nucleic Acids Res. 1999;27:1690-7 pubmed
    Although the G+C content of Thermus aquaticus YT-1 chromosomal DNA is 67.4%, regions with lower G+C content have also been observed...
  58. Padmanabhan S, Freymann D. The conformation of bound GMPPNP suggests a mechanism for gating the active site of the SRP GTPase. Structure. 2001;9:859-67 pubmed
    ..The structural basis for the GTP-dependent interaction between the two proteins, however, remains unknown...
  59. Koh S, Kim J, Shin H, Lee D, Bae J, Kim D, et al. Mechanistic study of the intramolecular conversion of maltose to trehalose by Thermus caldophilus GK24 trehalose synthase. Carbohydr Res. 2003;338:1339-43 pubmed
    ..of molecular transformation are involved in the conversion of maltose to trehalose by trehalose synthase from Thermus caldophilus GK24...
  60. Feklistov A, Barinova N, Sevostyanova A, Heyduk E, Bass I, Vvedenskaya I, et al. A basal promoter element recognized by free RNA polymerase sigma subunit determines promoter recognition by RNA polymerase holoenzyme. Mol Cell. 2006;23:97-107 pubmed
    ..We selected ssDNA aptamers that strongly and specifically bound free sigma(A) from Thermus aquaticus...
  61. Hreggvidsson G, Skirnisdottir S, Smit B, Hjorleifsdottir S, Marteinsson V, Petursdottir S, et al. Polyphasic analysis of Thermus isolates from geothermal areas in Iceland. Extremophiles. 2006;10:563-75 pubmed
    Genetic relationships and diversity of 101 Thermus isolates from different geothermal regions in Iceland were investigated by using multilocus enzyme electrophoresis (MLEE) and small subunit ribosomal rRNA (SSU rRNA) sequence analysis...
  62. Son J, Kim H, Oh D. Ginsenoside Rd production from the major ginsenoside Rb(1) by beta-glucosidase from Thermus caldophilus. Biotechnol Lett. 2008;30:713-6 pubmed
    ..Rd was produced from 5 mg reagent-grade ginsenoside Rb(1) in 5 ml after 30 min by beta-glucosidase from Thermus caldophilus GK24. Using a ginseng root extract containing 1 mg ginsenoside Rb(1) ml(-1) and 3...
  63. Tong J, Cao W, Barany F. Biochemical properties of a high fidelity DNA ligase from Thermus species AK16D. Nucleic Acids Res. 1999;27:788-94 pubmed
    NAD+-dependent DNA ligases from thermophilic bacteria Thermus species are highly homologous with amino acid sequence identities ranging from 85 to 98%...
  64. Kieft T, Fredrickson J, Onstott T, Gorby Y, Kostandarithes H, Bailey T, et al. Dissimilatory reduction of Fe(III) and other electron acceptors by a Thermus isolate. Appl Environ Microbiol. 1999;65:1214-21 pubmed
    ..This organism, designated SA-01, clustered most closely with members of the genus Thermus, as determined by 16S rRNA gene (rDNA) sequence analysis...
  65. Huang Y, Ito J. DNA polymerase C of the thermophilic bacterium Thermus aquaticus: classification and phylogenetic analysis of the family C DNA polymerases. J Mol Evol. 1999;48:756-69 pubmed
    ..We have sequenced the structural gene encoding the DNA pol C catalytic subunit of the thermophilic bacterium Thermus aquaticus...
  66. Jiricny J. Mismatch repair: the praying hands of fidelity. Curr Biol. 2000;10:R788-90 pubmed
    ..crystal structures have recently been solved for the mismatch binding protein MutS of Escherichia coli and its Thermus aquaticus homologue; they show how these factors recognise such structurally diverse substrates as base-base ..
  67. Pessela B, Mateo C, Carrascosa A, Vian A, Garcia J, Rivas G, et al. One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-His-tagged beta-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate-epoxy Sepabeads. Biomacromolecules. 2003;4:107-13 pubmed
    Using the poly-His-tagged-beta-galactosidase from Thermus sp...
  68. Karantzeni I, Ruiz C, Liu C, LiCata V. Comparative thermal denaturation of Thermus aquaticus and Escherichia coli type 1 DNA polymerases. Biochem J. 2003;374:785-92 pubmed
    Thermal denaturations of the type 1 DNA polymerases from Thermus aquaticus (Taq polymerase) and Escherichia coli (Pol 1) have been examined using differential scanning calorimetry and CD spectroscopy...
  69. Balkwill D, Kieft T, Tsukuda T, Kostandarithes H, Onstott T, Macnaughton S, et al. Identification of iron-reducing Thermus strains as Thermus scotoductus. Extremophiles. 2004;8:37-44 pubmed
    b>Thermus strain SA-01, previously isolated from a deep (3.2 km) South African gold mine, is closely related to Thermus strains NMX2 A.1 and VI-7 (previously isolated from thermal springs in New Mexico, USA, and Portugal, respectively)...
  70. Zenkin N, Yuzenkova Y, Severinov K. Transcript-assisted transcriptional proofreading. Science. 2006;313:518-20 pubmed
    ..We show that during transcription elongation, the hydrolytic reaction stimulated by misincorporated nucleotides proofreads most of the misincorporation events and thus serves as an intrinsic mechanism of transcription fidelity...
  71. Strerath M, Gloeckner C, Liu D, Schnur A, Marx A. Directed DNA polymerase evolution: effects of mutations in motif C on the mismatch-extension selectivity of thermus aquaticus DNA polymerase. Chembiochem. 2007;8:395-401 pubmed
    ..Here we present results obtained from amino acid randomization within one motif, motif C, of thermostable Thermus aquaticus DNA polymerase...
  72. Bester P, Litthauer D, Piater L, van Heerden E. A thioredoxin reductase-like protein from the thermophile, Thermus scotoductus SA-01, displaying iron reductase activity. FEMS Microbiol Lett. 2010;302:182-8 pubmed publisher
    ..The cytoplasmic NAD(P)H-dependent ferric reductase in Thermus scotoductus SA-01 shares high sequence and structural similarity to prokaryotic thioredoxin reductases...
  73. Zhang G, Campbell E, Minakhin L, Richter C, Severinov K, Darst S. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution. Cell. 1999;98:811-24 pubmed
    The X-ray crystal structure of Thermus aquaticus core RNA polymerase reveals a "crab claw"-shaped molecule with a 27 A wide internal channel...
  74. Shepotinovskaya I, Freymann D. Conformational change of the N-domain on formation of the complex between the GTPase domains of Thermus aquaticus Ffh and FtsY. Biochim Biophys Acta. 2002;1597:107-14 pubmed
    ..association of the 'NG' GTPase domains of the prokaryotic homologs of the SRP components, Ffh and FtsY, from Thermus aquaticus...
  75. Feitkenhauer H, Müller R, Märkl H. Degradation of polycyclic aromatic hydrocarbons and long chain alkanes at 60-70 degrees C by Thermus and Bacillus spp [corrected]. Biodegradation. 2003;14:367-72 pubmed
    ..Mixed and pure cultures of aerobic, extreme thermophilic microorganisms (Bacillus spp., Thermus sp.) were used to degrade PAH compounds and PAH/alkane mixtures at 65 degrees C...
  76. Kuznedelov K, Minakhin L, Severinov K. Preparation and characterization of recombinant Thermus aquaticus RNA polymerase. Methods Enzymol. 2003;370:94-108 pubmed
  77. Focia P, Gawronski Salerno J, Coon J, Freymann D. Structure of a GDP:AlF4 complex of the SRP GTPases Ffh and FtsY, and identification of a peripheral nucleotide interaction site. J Mol Biol. 2006;360:631-43 pubmed
    ..This site exhibits conserved sequence and structural features that suggest a direct interaction with RNA plays a role in regulating the activity of the SRP targeting complex...
  78. Lin M, Yang Y, Chen Y, Hua K, Lu C, Sheu F, et al. A novel exopolysaccharide from the biofilm of Thermus aquaticus YT-1 induces the immune response through Toll-like receptor 2. J Biol Chem. 2011;286:17736-45 pubmed publisher
    ..Here we isolated a novel extracellular polysaccharide from the biofilm of Thermus aquaticus YT-1 and evaluated its structure and immunomodulatory effects...
  79. Kelly C, Nishiyama M, Ohnishi Y, Beppu T, Birktoft J. Determinants of protein thermostability observed in the 1.9-A crystal structure of malate dehydrogenase from the thermophilic bacterium Thermus flavus. Biochemistry. 1993;32:3913-22 pubmed
    A binary complex of malate dehydrogenase from the thermophilic bacterium Thermus flavus (tMDH) with NADH has been crystallized from poly(ethylene glycol) 3500, pH 8.5, yielding diffraction-quality crystals in space group P2(1)2(1)2(1)...
  80. Biswas I, Hsieh P. Identification and characterization of a thermostable MutS homolog from Thermus aquaticus. J Biol Chem. 1996;271:5040-8 pubmed
    ..Here, we describe the isolation and characterization of a thermostable MutS homolog from Thermus aquaticus YT-1. Sequencing of the mutS gene predicts an 89...
  81. Tsusaki K, Nishimoto T, Nakada T, Kubota M, Chaen H, Fukuda S, et al. Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC33923. Biochim Biophys Acta. 1997;1334:28-32 pubmed
    ..synthase (catalyzing the conversion of maltose into alpha, alpha-trehalose by transglucosylation) was cloned from Thermus aquaticus ATCC33923. Sequence analysis revealed a 2892 bp synthase gene and a 963 residue amino-acid sequence...
  82. Gihring T, Banfield J. Arsenite oxidation and arsenate respiration by a new Thermus isolate. FEMS Microbiol Lett. 2001;204:335-40 pubmed
    ..The isolate, designated HR13, was identified as a Thermus species based on 16S rDNA phylogenetic relationships and close sequence similarity within the Thermus genus...
  83. Kurosawa N, Itoh Y, Itoh T. Thermus kawarayensis sp. nov., a new member of the genus Thermus, isolated from Japanese hot springs. Extremophiles. 2005;9:81-4 pubmed
    ..Cloning and preliminary sequence analysis of 16S rDNA showed that this isolate belongs to the genus Thermus. The cells were 10-20 microm long, about 0...
  84. Park S, Kang H, Shim J, Woo E, Hong J, Kim J, et al. Modulation of substrate preference of thermus maltogenic amylase by mutation of the residues at the interface of a dimer. Biosci Biotechnol Biochem. 2007;71:1564-7 pubmed
    To elucidate the relationship between the substrate size and geometric shape of the catalytic site of Thermus maltogenic amylase, Gly50, Asp109, and Val431, located at the interface of the dimer, were replaced with bulky amino acids...
  85. Zylicz Stachula A, Zołnierkiewicz O, Sliwińska K, Jezewska Frackowiak J, Skowron P. Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving. BMC Biochem. 2011;12:62 pubmed publisher
    The TaqII enzyme is a member of the Thermus sp. enzyme family that we propounded previously within Type IIS restriction endonucleases, containing related thermophilic bifunctional endonucleases-methyltransferases from various Thermus sp...
  86. Diaz R, Sabino E. Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase. Braz J Med Biol Res. 1998;31:1239-42 pubmed
    ..The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that would survive the high temperatures needed for DNA denaturation...
  87. Kaplan D, Steitz T. DnaB from Thermus aquaticus unwinds forked duplex DNA with an asymmetric tail length dependence. J Biol Chem. 1999;274:6889-97 pubmed
    ..The dnaB gene from Thermus aquaticus was isolated and cloned, and its gene product was expressed and purified to homogeneity...
  88. Egea P, Shan S, Napetschnig J, Savage D, Walter P, Stroud R. Substrate twinning activates the signal recognition particle and its receptor. Nature. 2004;427:215-21 pubmed
    ..This unique circle of twinned interactions is severed twice on hydrolysis, leading to complex dissociation after cargo delivery...
  89. Kuznedelov K, Lamour V, Patikoglou G, Chlenov M, Darst S, Severinov K. Recombinant Thermus aquaticus RNA polymerase for structural studies. J Mol Biol. 2006;359:110-21 pubmed publisher
    ..of bacterial transcription have come from studies of RNA polymerases (RNAPs) from the thermophilic eubacteria Thermus aquaticus (Taq) and Thermus thermophilus (Tth)...
  90. Cho M, Chung S, Heo S, Ku J, Ban C. A simple fluorescent method for detecting mismatched DNAs using a MutS-fluorophore conjugate. Biosens Bioelectron. 2007;22:1376-81 pubmed
    ..naphthalene-6-sulfonic acid (IAANS), was site-specifically attached to the 469 position of Thermus aquaticus (Taq.) MutS mutant (C42A/T469C)...
  91. Miropolskaya N, Artsimovitch I, Klimasauskas S, Nikiforov V, Kulbachinskiy A. Allosteric control of catalysis by the F loop of RNA polymerase. Proc Natl Acad Sci U S A. 2009;106:18942-7 pubmed publisher
    ..At moderate temperatures, the rate of catalysis by RNAP from thermophilic Thermus aquaticus is dramatically reduced compared with its closest mesophilic relative, Deinococcus radiodurans...
  92. Yuzenkova Y, Zenkin N. Central role of the RNA polymerase trigger loop in intrinsic RNA hydrolysis. Proc Natl Acad Sci U S A. 2010;107:10878-83 pubmed publisher
    ..Here, by using Thermus aquaticus RNA polymerase, we show that the reaction also requires the flexible domain of the active center, the ..