rhodospirillum rubrum

Summary

Summary: Vibrio- to spiral-shaped phototrophic bacteria found in stagnant water and mud exposed to light.

Top Publications

  1. Davis R, Lehman L, Petrovich R, Shah V, Roberts G, Ludden P. Purification and characterization of the alternative nitrogenase from the photosynthetic bacterium Rhodospirillum rubrum. J Bacteriol. 1996;178:1445-50 pubmed
    The alternative nitrogenase from a nifH mutant of the photosynthetic bacterium Rhodospirillum rubrum has been purified and characterized...
  2. Amarie S, Lupo D, Lenz M, Saegesser R, Ghosh R, Wachtveitl J. Excitation energy pathways in the photosynthetic units of reaction center LM- and H-subunit deletion mutants of Rhodospirillum rubrum. Photosynth Res. 2010;103:141-51 pubmed publisher
    ..H-subunit deletion mutant) and SK Delta LM (an (L+M) deletion mutant) of the purple non-sulphur bacterium Rhodospirillum rubrum have been investigated by femtosecond transient absorption spectroscopy...
  3. Reslewic S, Zhou S, Place M, Zhang Y, Briska A, Goldstein S, et al. Whole-genome shotgun optical mapping of Rhodospirillum rubrum. Appl Environ Microbiol. 2005;71:5511-22 pubmed
    b>Rhodospirillum rubrum is a phototrophic purple nonsulfur bacterium known for its unique and well-studied nitrogen fixation and carbon monoxide oxidation systems and as a source of hydrogen and biodegradable plastic production...
  4. Edgren T, Nordlund S. Two pathways of electron transport to nitrogenase in Rhodospirillum rubrum: the major pathway is dependent on the fix gene products. FEMS Microbiol Lett. 2006;260:30-5 pubmed
    In the photosynthetic bacterium Rhodospirillum rubrum, as in many other diazotrophs, electron transport to nitrogenase has not been characterized in great detail. In this study, we show that there are two pathways operating in R. rubrum...
  5. Aono S, Nakajima H, Saito K, Okada M. A novel heme protein that acts as a carbon monoxide-dependent transcriptional activator in Rhodospirillum rubrum. Biochem Biophys Res Commun. 1996;228:752-6 pubmed
    The gene coding for a carbon monoxide-dependent transcriptional activator (cooA) in Rhodospirillum rubrum has been expressed in E. coli, and the recombinant CooA has been purified...
  6. Kessi J. Enzymic systems proposed to be involved in the dissimilatory reduction of selenite in the purple non-sulfur bacteria Rhodospirillum rubrum and Rhodobacter capsulatus. Microbiology. 2006;152:731-43 pubmed
    ..involvement of these different enzymes in the reduction of selenite in the purple non-sulfur bacteria Rhodospirillum rubrum and Rhodobacter capsulatus...
  7. Jeon W, Cheng J, Ludden P. Purification and characterization of membrane-associated CooC protein and its functional role in the insertion of nickel into carbon monoxide dehydrogenase from Rhodospirillum rubrum. J Biol Chem. 2001;276:38602-9 pubmed
    ..In this study, CooC was purified from the chromatophore membranes of Rhodospirillum rubrum with a 3,464-fold purification and a 0.8% recovery, and its biochemical properties were characterized...
  8. Fox J, He Y, Shelver D, Roberts G, Ludden P. Characterization of the region encoding the CO-induced hydrogenase of Rhodospirillum rubrum. J Bacteriol. 1996;178:6200-8 pubmed
    In the photosynthetic bacterium Rhodospirillum rubrum, the presence of carbon monoxide (CO) induces expression of several proteins. These include carbon monoxide dehydrogenase (CODH) and a CO-tolerant hydrogenase...
  9. Clemente T, Shah D, Tran M, Stark D, Padgette S, Dennis D, et al. Sequence of PHA synthase gene from two strains of Rhodospirillum rubrum and in vivo substrate specificity of four PHA synthases across two heterologous expression systems. Appl Microbiol Biotechnol. 2000;53:420-9 pubmed
    A 3.0-kb genomic fragment has been isolated from Rhodospirillum rubrum (ATCC 25903) that contains an open reading frame (ORF) with strong homology to other known polyhydroxyalkanoate (PHA) synthase genes...

More Information

Publications76

  1. Bahatyrova S, Frese R, Van Der Werf K, Otto C, Hunter C, Olsen J. Flexibility and size heterogeneity of the LH1 light harvesting complex revealed by atomic force microscopy: functional significance for bacterial photosynthesis. J Biol Chem. 2004;279:21327-33 pubmed
  2. Reiser S, Mitsky T, Gruys K. Characterization and cloning of an (R)-specific trans-2,3-enoylacyl-CoA hydratase from Rhodospirillum rubrum and use of this enzyme for PHA production in Escherichia coli. Appl Microbiol Biotechnol. 2000;53:209-18 pubmed
    An (R)-trans-2,3-enoylacyl-CoA hydratase was purified to near-homogeneity from Rhodospirillum rubrum. Protein sequencing of enriched protein fractions allowed the construction of a degenerate oligonucleotide...
  3. Singh J, Tabita F. Roles of RubisCO and the RubisCO-like protein in 5-methylthioadenosine metabolism in the Nonsulfur purple bacterium Rhodospirillum rubrum. J Bacteriol. 2010;192:1324-31 pubmed publisher
    ..Here, we show that the form II RubisCO enzyme from the nonsulfur purple bacterium Rhodospirillum rubrum is also able to function as an enolase in vivo as part of an MSP, but only under anaerobic conditions...
  4. Imker H, Singh J, Warlick B, Tabita F, Gerlt J. Mechanistic diversity in the RuBisCO superfamily: a novel isomerization reaction catalyzed by the RuBisCO-like protein from Rhodospirillum rubrum. Biochemistry. 2008;47:11171-3 pubmed publisher
    ..The RLP from Rhodospirillum rubrum (gi:83593333) catalyzes a novel isomerization reaction (overall 1,3-proton transfer reaction; likely, two ..
  5. Fox J, Kerby R, Roberts G, Ludden P. Characterization of the CO-induced, CO-tolerant hydrogenase from Rhodospirillum rubrum and the gene encoding the large subunit of the enzyme. J Bacteriol. 1996;178:1515-24 pubmed
    In the presence of carbon monoxide, the photosynthetic bacterium Rhodospirillum rubrum induces expression of proteins which allow the organism to metabolize carbon monoxide in the net reaction CO + H2O --> CO2 + H2...
  6. Butzin N, Owen H, Collins M. A new system for heterologous expression of membrane proteins: Rhodospirillum rubrum. Protein Expr Purif. 2010;70:88-94 pubmed publisher
    ..production of heterologous membrane proteins based on a mutant of the facultatively phototrophic bacterium Rhodospirillum rubrum. Under certain growth conditions, R...
  7. Brajcich B, Iarocci A, Johnstone L, Morgan R, Lonjers Z, Hotchko M, et al. Evidence that ubiquinone is a required intermediate for rhodoquinone biosynthesis in Rhodospirillum rubrum. J Bacteriol. 2010;192:436-45 pubmed publisher
    Rhodoquinone (RQ) is an important cofactor used in the anaerobic energy metabolism of Rhodospirillum rubrum. RQ is structurally similar to ubiquinone (coenzyme Q or Q), a polyprenylated benzoquinone used in the aerobic respiratory chain...
  8. Jonsson A, Nordlund S. In vitro studies of the uridylylation of the three PII protein paralogs from Rhodospirillum rubrum: the transferase activity of R. rubrum GlnD is regulated by alpha-ketoglutarate and divalent cations but not by glutamine. J Bacteriol. 2007;189:3471-8 pubmed
    ..The regulation of this enzyme is thus of central importance. In Rhodospirillum rubrum, three P(II) paralogs have been identified...
  9. Jonsson A, Teixeira P, Nordlund S. The activity of adenylyltransferase in Rhodospirillum rubrum is only affected by alpha-ketoglutarate and unmodified PII proteins, but not by glutamine, in vitro. FEBS J. 2007;274:2449-60 pubmed
    ..We have investigated the regulation of ATase from Rhodospirillum rubrum, a photosynthetic nitrogen-fixing bacterium...
  10. Grammel H, Gilles E, Ghosh R. Microaerophilic cooperation of reductive and oxidative pathways allows maximal photosynthetic membrane biosynthesis in Rhodospirillum rubrum. Appl Environ Microbiol. 2003;69:6577-86 pubmed
    The purple nonsulfur bacterium Rhodospirillum rubrum has been employed to study physiological adaptation to limiting oxygen tensions (microaerophilic conditions). R...
  11. Addlesee H, Hunter C. Rhodospirillum rubrum possesses a variant of the bchP gene, encoding geranylgeranyl-bacteriopheophytin reductase. J Bacteriol. 2002;184:1578-86 pubmed
    ..In contrast, we demonstrate that a newly identified homolog of this gene in Rhodospirillum rubrum encodes an enzyme, GG-Bphe reductase, capable of reducing the isoprenoid moiety of Bphe only...
  12. Heo J, Skjeldal L, Staples C, Ludden P. Carbon monoxide dehydrogenase from Rhodospirillum rubrum produces formate. J Biol Inorg Chem. 2002;7:810-4 pubmed
    Carbon monoxide dehydrogenase (CODH) from Rhodospirillum rubrum reversibly catalyzes the oxidation of CO to CO(2) at the active site C-cluster...
  13. Lee D, Oh D, Oh Y, Malinverni J, Kukor J, Kahng H. Cloning and characterization of monofunctional catalase from photosynthetic bacterium Rhodospirillum rubrum S1. J Microbiol Biotechnol. 2007;17:1460-8 pubmed
    In this study, an approx. 2.5-kb gene fragment including the catalase gene from Rhodospirillum rubrum S1 was cloned and characterized...
  14. Zhang Y, Pohlmann E, Roberts G. Identification of critical residues in GlnB for its activation of NifA activity in the photosynthetic bacterium Rhodospirillum rubrum. Proc Natl Acad Sci U S A. 2004;101:2782-7 pubmed
    ..Three P(II) homologs called GlnB, GlnK, and GlnJ have been identified in the photosynthetic bacterium Rhodospirillum rubrum. These have roles in at least four distinct functions, one of which is activation of the nitrogen fixation-..
  15. Zou X, Zhu Y, Pohlmann E, Li J, Zhang Y, Roberts G. Identification and functional characterization of NifA variants that are independent of GlnB activation in the photosynthetic bacterium Rhodospirillum rubrum. Microbiology. 2008;154:2689-99 pubmed publisher
    ..the well-studied NifL-NifA regulatory systems in Klebsiella pneumoniae and Azotobacter vinelandii, in Rhodospirillum rubrum NifA is activated by a direct protein-protein interaction with the uridylylated form of GlnB, which in ..
  16. Favier Teodorescu L, Cornet J, Dussap C. Modelling continuous culture of Rhodospirillum rubrum in photobioreactor under light limited conditions. Biotechnol Lett. 2003;25:359-64 pubmed
    b>Rhodospirillum rubrum was grown continuously and photoheterotrophically under light limitation using a cylindrical photobioreactor in which the steady state biomass concentration was varied between 0...
  17. Berg I, Filatova L, Ivanovsky R. Inhibition of acetate and propionate assimilation by itaconate via propionyl-CoA carboxylase in isocitrate lyase-negative purple bacterium Rhodospirillum rubrum. FEMS Microbiol Lett. 2002;216:49-54 pubmed
    ..inhibitor of acetate and propionate assimilation in isocitrate lyase-negative purple non-sulfur bacterium Rhodospirillum rubrum. It was shown that in cell extracts of R...
  18. Carius A, Henkel M, Grammel H. A glutathione redox effect on photosynthetic membrane expression in Rhodospirillum rubrum. J Bacteriol. 2011;193:1893-900 pubmed publisher
    ..In the present study we demonstrate that in Rhodospirillum rubrum, the amount of photosynthetic membranes can be drastically elevated by external supplementation of the ..
  19. Nakano T, Ashida H, Mizohata E, Matsumura H, Yokota A. An evolutionally conserved Lys122 is essential for function in Rhodospirillum rubrum bona fide RuBisCO and Bacillus subtilis RuBisCO-like protein. Biochem Biophys Res Commun. 2010;392:212-6 pubmed publisher
    ..We used site-directed mutagenesis to analyze the function of Lys122 in the form II Rhodospirillum rubrum RuBisCO (RrRuBisCO) and Bacillus subtilis RLP (BsRLP)...
  20. Kessi J, Ramuz M, Wehrli E, Spycher M, Bachofen R. Reduction of selenite and detoxification of elemental selenium by the phototrophic bacterium Rhodospirillum rubrum. Appl Environ Microbiol. 1999;65:4734-40 pubmed
    ..of cultures to reduce selenite, and the mechanism of detoxification of selenium were investigated by using Rhodospirillum rubrum. Anoxic photosynthetic cultures were able to completely reduce as much as 1...
  21. Zhang Y, Pohlmann E, Serate J, Conrad M, Roberts G. Mutagenesis and functional characterization of the four domains of GlnD, a bifunctional nitrogen sensor protein. J Bacteriol. 2010;192:2711-21 pubmed publisher
    ..Here we report the functional analysis of these domains of GlnD from Escherichia coli and Rhodospirillum rubrum. We confirm the assignment of UTase activity to the NT domain and show that the UR activity is a property ..
  22. Jamieson S, Wang P, Qian P, Kirkland J, Conroy M, Hunter C, et al. Projection structure of the photosynthetic reaction centre-antenna complex of Rhodospirillum rubrum at 8.5 A resolution. EMBO J. 2002;21:3927-35 pubmed
    ..crystals of the reaction-centre-light-harvesting complex I (RC-LH1) of the purple non- sulfur bacterium Rhodospirillum rubrum have been formed from detergent-solubilized and purified protein complexes...
  23. Warlick B, Imker H, Sriram J, Tabita F, Gerlt J. Mechanistic diversity in the RuBisCO superfamily: RuBisCO from Rhodospirillum rubrum is not promiscuous for reactions catalyzed by RuBisCO-like proteins. Biochemistry. 2012;51:9470-9 pubmed publisher
    ..shunt" that generates 1-deoxy-d-xylulose 5-phosphate for nonmevalonate isoprene synthesis in Rhodospirillum rubrum. We first studied the structure and reactivity of DK-MTP 1-P that was reported to decompose rapidly [..
  24. Nakagawa K, Suzuki S, Fujii R, Gardiner A, Cogdell R, Nango M, et al. Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy. Photosynth Res. 2008;95:339-44 pubmed
    ..4 x 10(5) [V/cm]. This change can explain the 3 nm wavelength shift of the Q(y) absorption band in the reconstituted LH1 complex...
  25. Shelver D, Kerby R, He Y, Roberts G. Carbon monoxide-induced activation of gene expression in Rhodospirillum rubrum requires the product of cooA, a member of the cyclic AMP receptor protein family of transcriptional regulators. J Bacteriol. 1995;177:2157-63 pubmed
    Induction of the CO-oxidizing system of the photosynthetic bacterium Rhodospirillum rubrum is regulated at the level of gene expression by the presence of CO...
  26. Mastroleo F, Leroy B, Van Houdt R, s Heeren C, Mergeay M, Hendrickx L, et al. Shotgun proteome analysis of Rhodospirillum rubrum S1H: integrating data from gel-free and gel-based peptides fractionation methods. J Proteome Res. 2009;8:2530-41 pubmed publisher
    ..and gel-based peptides fractionation steps, we identified 932 proteins from the alpha-proteobacterium Rhodospirillum rubrum S1H...
  27. Do Y, Smeenk J, Broer K, Kisting C, Brown R, Heindel T, et al. Growth of Rhodospirillum rubrum on synthesis gas: conversion of CO to H2 and poly-beta-hydroxyalkanoate. Biotechnol Bioeng. 2007;97:279-86 pubmed
    To examine the potential use of synthesis gas as a carbon and energy source in fermentation processes, Rhodospirillum rubrum was cultured on synthesis gas generated from discarded seed corn...
  28. Berthold C, Wang H, Nordlund S, Högbom M. Mechanism of ADP-ribosylation removal revealed by the structure and ligand complexes of the dimanganese mono-ADP-ribosylhydrolase DraG. Proc Natl Acad Sci U S A. 2009;106:14247-52 pubmed publisher
    ..In the phototrophic bacterium Rhodospirillum rubrum, dinitrogenase reductase-activating glycohydrolase (DraG), a dimanganese enzyme that reversibly associates ..
  29. Wang H, Noren A. Metabolic regulation of nitrogen fixation in Rhodospirillum rubrum. Biochem Soc Trans. 2006;34:160-1 pubmed
    Nitrogenase activity in Rhodospirillum rubrum is post-translationally regulated by DRAG (dinitrogenase reductase glycohydrolase) and DRAT (dinitrogenase reductase ADP-ribosylation transferase)...
  30. Zhang Y, Pohlmann E, Ludden P, Roberts G. Functional characterization of three GlnB homologs in the photosynthetic bacterium Rhodospirillum rubrum: roles in sensing ammonium and energy status. J Bacteriol. 2001;183:6159-68 pubmed
    ..P(II)) protein, the product of glnB, has been characterized previously in the photosynthetic bacterium Rhodospirillum rubrum. Here we describe identification of two other P(II) homologs in this organism, GlnK and GlnJ...
  31. Neumann U, Schiltz E, Stahl B, Hillenkamp F, Weckesser J. A peptidoglycan binding domain in the porin-associated protein (PAP) of Rhodospirillum rubrum FR1. FEMS Microbiol Lett. 1996;138:55-8 pubmed
    The porin-associated protein of Rhodospirillum rubrum FR1 was found to contain a peptidoglycan binding motif. A partial fragment of 179 amino acids, obtained by cleavage of PAP with trypsin, Asp-N protease, and CNBr, was sequenced...
  32. Handrick R, Reinhardt S, Kimmig P, Jendrossek D. The "intracellular" poly(3-hydroxybutyrate) (PHB) depolymerase of Rhodospirillum rubrum is a periplasm-located protein with specificity for native PHB and with structural similarity to extracellular PHB depolymerases. J Bacteriol. 2004;186:7243-53 pubmed
    b>Rhodospirillum rubrum possesses a putative intracellular poly(3-hydroxybutyrate) (PHB) depolymerase system consisting of a soluble PHB depolymerase, a heat-stable activator, and a 3-hydroxybutyrate dimer hydrolase (J. M. Merrick and M...
  33. Maness P, Weaver P. Evidence for three distinct hydrogenase activities in Rhodospirillum rubrum. Appl Microbiol Biotechnol. 2001;57:751-6 pubmed
    Inducer, inhibitor, and mutant studies on three hydrogenase activities of Rhodospirillum rubrum indicate that they are mediated by three distinct hydrogenase enzymes...
  34. Zhang Y, Pohlmann E, Conrad M, Roberts G. The poor growth of Rhodospirillum rubrum mutants lacking PII proteins is due to an excess of glutamine synthetase activity. Mol Microbiol. 2006;61:497-510 pubmed publisher
    ..We have analysed a number of the suppressor mutations that correct such growth problems in Rhodospirillum rubrum mutants lacking P(II) proteins...
  35. Kessi J, Hanselmann K. Similarities between the abiotic reduction of selenite with glutathione and the dissimilatory reaction mediated by Rhodospirillum rubrum and Escherichia coli. J Biol Chem. 2004;279:50662-9 pubmed
    ..Experiments with the phototrophic alpha proteobacterium Rhodospirillum rubrum showed that the rate of selenite reduction was decreased when bacteria synthesized lower than normal ..
  36. Seufferheld M, Lea C, Vieira M, Oldfield E, Docampo R. The H(+)-pyrophosphatase of Rhodospirillum rubrum is predominantly located in polyphosphate-rich acidocalcisomes. J Biol Chem. 2004;279:51193-202 pubmed
    ..In this work, we report that the H(+)-pyrophosphatase (H(+)-PPase) of Rhodospirillum rubrum, the first enzyme of this type that was identified and thought to be localized only to chromatophore ..
  37. Wang G, Grammel H, Abou Aisha K, Sägesser R, Ghosh R. High-level production of the industrial product lycopene by the photosynthetic bacterium Rhodospirillum rubrum. Appl Environ Microbiol. 2012;78:7205-15 pubmed
    The biosynthesis of the major carotenoid spirilloxanthin by the purple nonsulfur bacterium Rhodospirillum rubrum is thought to occur via a linear pathway proceeding through phytoene and, later, lycopene as intermediates...
  38. L pez Marqu s R, P rez Casti eira J, Losada M, Serrano A. Differential regulation of soluble and membrane-bound inorganic pyrophosphatases in the photosynthetic bacterium Rhodospirillum rubrum provides insights into pyrophosphate-based stress bioenergetics. J Bacteriol. 2004;186:5418-26 pubmed publisher
    ..inorganic pyrophosphatases (sPPase and H(+)-PPase, respectively) of the purple nonsulfur bacterium Rhodospirillum rubrum are differentially regulated by environmental growth conditions...
  39. Baltscheffsky M, Nadanaciva S, Schultz A. A pyrophosphate synthase gene: molecular cloning and sequencing of the cDNA encoding the inorganic pyrophosphate synthase from Rhodospirillum rubrum. Biochim Biophys Acta. 1998;1364:301-6 pubmed
    ..The deduced protein contains 660 amino acid residues and 15 putative membrane-spanning segments. It is homologous to the vacuolar pyrophosphatases from plants...
  40. Drennan C, Heo J, Sintchak M, Schreiter E, Ludden P. Life on carbon monoxide: X-ray structure of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase. Proc Natl Acad Sci U S A. 2001;98:11973-8 pubmed publisher
    A crystal structure of the anaerobic Ni-Fe-S carbon monoxide dehydrogenase (CODH) from Rhodospirillum rubrum has been determined to 2.8-A resolution. The CODH family, for which the R...
  41. Wolfe D, Zhang Y, Roberts G. Specificity and regulation of interaction between the PII and AmtB1 proteins in Rhodospirillum rubrum. J Bacteriol. 2007;189:6861-9 pubmed publisher
    ..The regulation of their interaction is only partially understood, and we show that in Rhodospirillum rubrum one P(II) homolog, GlnJ, has higher affinity for an AmtB(1)-containing membrane than the other two P(II) ..
  42. Fotiadis D, Qian P, Philippsen A, Bullough P, Engel A, Hunter C. Structural analysis of the reaction center light-harvesting complex I photosynthetic core complex of Rhodospirillum rubrum using atomic force microscopy. J Biol Chem. 2004;279:2063-8 pubmed
    The bacterium Rhodospirillum rubrum contains a simple photosynthetic system, in which the reaction center (RC) receives energy from the light-harvesting (LH1) complex...
  43. Fitzmaurice W, Saari L, Lowery R, Ludden P, Roberts G. Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum. Mol Gen Genet. 1989;218:340-7 pubmed
    Nitrogen fixation activity in the photosynthetic bacterium Rhodospirillum rubrum is controlled by the reversible ADP-ribosylation of the dinitrogenase reductase component of the nitrogenase enzyme complex...
  44. Mastroleo F, Van Houdt R, Leroy B, Benotmane M, Janssen A, Mergeay M, et al. Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight. ISME J. 2009;3:1402-19 pubmed publisher
    ..In that purpose, the alpha-proteobacterium, Rhodospirillum rubrum S1H, was sent twice to the International Space Station and was analyzed post-flight using a newly ..
  45. Pycke B, Crabbé A, Verstraete W, Leys N. Characterization of triclosan-resistant mutants reveals multiple antimicrobial resistance mechanisms in Rhodospirillum rubrum S1H. Appl Environ Microbiol. 2010;76:3116-23 pubmed publisher
    ..mechanisms were identified in 11 spontaneous high- and low-level triclosan resistance (Tcs(r)) mutants of Rhodospirillum rubrum S1H by genotyping complemented with transcriptional analyses, antibiotic resistance screening, and ..
  46. Zeiger L, Grammel H. Model-based high cell density cultivation of Rhodospirillum rubrum under respiratory dark conditions. Biotechnol Bioeng. 2010;105:729-39 pubmed publisher
    ..in common bioreactors offers a new perspective for utilizing the facultative photosynthetic bacterium Rhodospirillum rubrum for large-scale applications...
  47. Selao T, Nordlund S, Norén A. Comparative proteomic studies in Rhodospirillum rubrum grown under different nitrogen conditions. J Proteome Res. 2008;7:3267-75 pubmed publisher
    Forty-four differentially expressed proteins have been identified in the photosynthetic diazotroph Rhodospirillum rubrum grown anaerobic and photoheterotrophically, with different nitrogen sources, using 2D-PAGE and MALDI-TOF, from gels ..
  48. Singer S, Hirst M, Ludden P. CO-dependent H2 evolution by Rhodospirillum rubrum: role of CODH:CooF complex. Biochim Biophys Acta. 2006;1757:1582-91 pubmed
    Upon exposure to CO during anaerobic growth, the purple phototrophic bacterium Rhodospirillum rubrum expresses a CO-oxidizing H(2) evolving enzymatic system...
  49. Korszun Z, Bunker G, Khalid S, Scheidt W, Cusanovich M, Meyer T. Extended X-ray absorption fine structure study of Rhodospirillum rubrum and Rhodospirillum molischianum cytochromes c': relationship between heme stereochemistry and spin state. Biochemistry. 1989;28:1513-7 pubmed
    An EXAFS study on the oxidized and reduced forms of cytochromes c' from Rhodospirillum rubrum and Rhodospirillum molischianum was performed at pH 7. The cytochromes c' have an apparent coordination number of 5 in both oxidation states...
  50. Salemme F, Freer S, Xuong N, Alden R, Kraut J. The structure of oxidized cytochrome c 2 of Rhodospirillum rubrum. J Biol Chem. 1973;248:3910-21 pubmed
  51. Wang H, Franke C, Nordlund S, Norén A. Reversible membrane association of dinitrogenase reductase activating glycohydrolase in the regulation of nitrogenase activity in Rhodospirillum rubrum; dependence on GlnJ and AmtB1. FEMS Microbiol Lett. 2005;253:273-9 pubmed
    In the photosynthetic bacterium Rhodospirillum rubrum nitrogenase activity is regulated by reversible ADP-ribosylation of dinitrogenase reductase in response to external so called "switch-off" effectors...
  52. He Y, Shelver D, Kerby R, Roberts G. Characterization of a CO-responsive transcriptional activator from Rhodospirillum rubrum. J Biol Chem. 1996;271:120-3 pubmed
    In Rhodospirillum rubrum, CO induces the expression of at least two transcripts that encode an enzyme system for CO oxidation...
  53. Edgren T, Nordlund S. The fixABCX genes in Rhodospirillum rubrum encode a putative membrane complex participating in electron transfer to nitrogenase. J Bacteriol. 2004;186:2052-60 pubmed
    In our efforts to identify the components participating in electron transport to nitrogenase in Rhodospirillum rubrum, we used mini-Tn5 mutagenesis followed by metronidazole selection...
  54. Ashida H, Saito Y, Kojima C, Kobayashi K, Ogasawara N, Yokota A. A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO. Science. 2003;302:286-90 pubmed
    ..gene for this RLP had been disrupted, was rescued by the gene for RuBisCOfrom the photosynthetic bacterium Rhodospirillum rubrum. Thus, the photosynthetic RuBisCO from R...
  55. Zhang Y, Pohlmann E, Ludden P, Roberts G. Mutagenesis and functional characterization of the glnB, glnA, and nifA genes from the photosynthetic bacterium Rhodospirillum rubrum. J Bacteriol. 2000;182:983-92 pubmed
    Nitrogen fixation is tightly regulated in Rhodospirillum rubrum at two different levels: transcriptional regulation of nif expression and posttranslational regulation of dinitrogenase reductase by reversible ADP-ribosylation catalyzed by ..
  56. Harrold J, Woronowicz K, Lamptey J, Awong J, BAIRD J, Moshar A, et al. Functional interfacing of Rhodospirillum rubrum chromatophores to a conducting support for capture and conversion of solar energy. J Phys Chem B. 2013;117:11249-59 pubmed publisher
    ..In particular, intracytoplasmic membrane vesicles (chromatophores) from the purple bacterium Rhodospirillum rubrum provide a fully functional and robust photosynthetic apparatus, ideal for biophysical investigations of ..
  57. Nakagawa K, Suzuki S, Fujii R, Gardiner A, Cogdell R, Nango M, et al. Probing the effect of the binding site on the electrostatic behavior of a series of carotenoids reconstituted into the light-harvesting 1 complex from purple photosynthetic bacterium Rhodospirillum rubrum detected by stark spectroscopy. J Phys Chem B. 2008;112:9467-75 pubmed publisher
    Reconstitutions of the LH1 complexes from the purple photosynthetic bacterium Rhodospirillum rubrum S1 were performed with a range of carotenoid molecules having different numbers of C=C conjugated double bonds...
  58. Carius L, Hädicke O, Grammel H. Stepwise reduction of the culture redox potential allows the analysis of microaerobic metabolism and photosynthetic membrane synthesis in Rhodospirillum rubrum. Biotechnol Bioeng. 2013;110:573-85 pubmed publisher
    ..The microaerobic lifestyle of anoxygenic photosynthetic bacteria like Rhodospirillum rubrum harbors such a phenomenon, as it allows the formation of photosynthetic membranes and related interesting ..
  59. Falcone D, Tabita F. Complementation analysis and regulation of CO2 fixation gene expression in a ribulose 1,5-bisphosphate carboxylase-oxygenase deletion strain of Rhodospirillum rubrum. J Bacteriol. 1993;175:5066-77 pubmed
    A ribulose 1,5-bisphosphate carboxylase-oxygenase (RubisCO) deletion strain of Rhodospirillum rubrum that was incapable of photolithoautotrophic growth was constructed. Photoheterotrophic growth, however, was possible for the R...
  60. Handrick R, Reinhardt S, Schultheiss D, Reichart T, Schüler D, Jendrossek V, et al. Unraveling the function of the Rhodospirillum rubrum activator of polyhydroxybutyrate (PHB) degradation: the activator is a PHB-granule-bound protein (phasin). J Bacteriol. 2004;186:2466-75 pubmed
    ..hydrolysis of native poly(3-hydroxybutyrate) (nPHB) granules in vitro by soluble PHB depolymerase of Rhodospirillum rubrum requires pretreatment of nPHB with an activator compound present in R. rubrum cells (J. M. Merrick and M...
  61. Zhang Y, Wolfe D, Pohlmann E, Conrad M, Roberts G. Effect of AmtB homologues on the post-translational regulation of nitrogenase activity in response to ammonium and energy signals in Rhodospirillum rubrum. Microbiology. 2006;152:2075-89 pubmed
    ..Three P(II) homologues, GlnB, GlnK and GlnJ, have been identified in the photosynthetic bacterium Rhodospirillum rubrum, and they have roles in at least four overlapping and distinct functions, one of which is the post-..
  62. Pearce F. Catalytic by-product formation and ligand binding by ribulose bisphosphate carboxylases from different phylogenies. Biochem J. 2006;399:525-34 pubmed publisher
    ..ID enzyme from the red alga Galdieria sulfuraria and the low-specificity Form II type from the bacterium Rhodospirillum rubrum all catalyse formation of by-products to varying degrees; however, the by-products are not inhibitory ..
  63. Teixeira P, Jonsson A, Frank M, Wang H, Nordlund S. Interaction of the signal transduction protein GlnJ with the cellular targets AmtB1, GlnE and GlnD in Rhodospirillum rubrum: dependence on manganese, 2-oxoglutarate and the ADP/ATP ratio. Microbiology. 2008;154:2336-47 pubmed publisher
    ..In the photosynthetic nitrogen-fixing bacterium Rhodospirillum rubrum three PII paralogues have been identified and termed GlnB, GlnJ and GlnK...
  64. Kerby R, Ludden P, Roberts G. In vivo nickel insertion into the carbon monoxide dehydrogenase of Rhodospirillum rubrum: molecular and physiological characterization of cooCTJ. J Bacteriol. 1997;179:2259-66 pubmed
    ..of cooCTJ are involved in normal in vivo Ni insertion into the carbon monoxide dehydrogenase (CODH) of Rhodospirillum rubrum. Located on a 1...
  65. Zhang Y, Pohlmann E, Roberts G. Effect of perturbation of ATP level on the activity and regulation of nitrogenase in Rhodospirillum rubrum. J Bacteriol. 2009;191:5526-37 pubmed publisher
    Nitrogenase activity in Rhodospirillum rubrum and in some other photosynthetic bacteria is regulated in part by the availability of light...
  66. Grammel H, Ghosh R. Redox-state dynamics of ubiquinone-10 imply cooperative regulation of photosynthetic membrane expression in Rhodospirillum rubrum. J Bacteriol. 2008;190:4912-21 pubmed publisher
    ..Here, we utilize Rhodospirillum rubrum to study the correlation of the quinone redox state to the expression level of photosynthetic membranes ..
  67. Kerby R, Hong S, Ensign S, Coppoc L, Ludden P, Roberts G. Genetic and physiological characterization of the Rhodospirillum rubrum carbon monoxide dehydrogenase system. J Bacteriol. 1992;174:5284-94 pubmed
    A 3.7-kb DNA region encoding part of the Rhodospirillum rubrum CO oxidation (coo) system was identified by using oligonucleotide probes...