azospirillum brasilense

Summary

Summary: A species of motile, free-living, gram-negative bacteria that occur in the soil. They are aerobic or microaerophilic and are sometimes capable of nitrogen fixation.

Top Publications

  1. Galindo Blaha C, Schrank I. An Azospirillum brasilense Tn5 mutant with modified stress response and impaired in flocculation. Antonie Van Leeuwenhoek. 2003;83:35-43 pubmed
    ..The pleiotrophic phenotype revealed for this mutant and the similarity of the C-terminal region of ORF280 to UspA from E. coli indicates that the A. brasilense ORF280 may be a Usp-like protein. ..
  2. Fischer S, Miguel M, Mori G. Effect of root exudates on the exopolysaccharide composition and the lipopolysaccharide profile of Azospirillum brasilense Cd under saline stress. FEMS Microbiol Lett. 2003;219:53-62 pubmed
    ..root exudates on the exopolysaccharide (EPS) composition and the lipopolysaccharide (LPS) profile of Azospirillum brasilense Cd under saline stress was studied. EPS of A...
  3. Kadouri D, Burdman S, Jurkevitch E, Okon Y. Identification and isolation of genes involved in poly(beta-hydroxybutyrate) biosynthesis in Azospirillum brasilense and characterization of a phbC mutant. Appl Environ Microbiol. 2002;68:2943-9 pubmed
    ..beta-ketothiolase), phbB (acetoacetyl coenzyme A reductase), and phbC (PHB synthase), were identified in Azospirillum brasilense strain Sp7, cloned, and sequenced...
  4. Belyakov A, Burygin G, Arbatsky N, Shashkov A, Selivanov N, Matora L, et al. Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp7. Carbohydr Res. 2012;361:127-32 pubmed publisher
    ..that the glycan chains of the polar flagellum flagellin of the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp7 are represented by a polysaccharide with a molecular mass of 7...
  5. Pothier J, Prigent Combaret C, Haurat J, Moënne Loccoz Y, Wisniewski Dyé F. Duplication of plasmid-borne nitrite reductase gene nirK in the wheat-associated plant growth-promoting rhizobacterium Azospirillum brasilense Sp245. Mol Plant Microbe Interact. 2008;21:831-42 pubmed publisher
    In the plant growth-promoting rhizobacterium Azospirillum brasilense Sp245, nitric oxide produced by denitrification could be a signal involved in stimulation of root branching, and the dissimilatory nitrite reductase gene nirK is ..
  6. Katsy E, Prilipov A. Mobile elements of an Azospirillum brasilense Sp245 85-MDa plasmid involved in replicon fusions. Plasmid. 2009;62:22-9 pubmed publisher
    Sequence analysis of approximately 25kb of an Azospirillum brasilense Sp245 85-MDa ( approximately 142kb) plasmid, p85, identified two novel IS elements mediating p85 fusions with a suicide plasmid vector, pJFF350...
  7. Assumpção M, de Souza E, Yates M, de Oliveira Pedrosa F, Benelli E. Purification and characterisation of Azospirillum brasilense N-truncated NtrX protein. Protein Expr Purif. 2007;53:302-8 pubmed
    ..To over-express and purify this protein, the ntrX gene of Azospirillum brasilense lacking the first eight codons was cloned into the vector pET29a+...
  8. Gupta N, Kumar S, Mishra M, Tripathi A. A constitutively expressed pair of rpoE2-chrR2 in Azospirillum brasilense Sp7 is required for survival under antibiotic and oxidative stress. Microbiology. 2013;159:205-18 pubmed publisher
    ..The Azospirillum brasilense Sp245 genome harbours nine genes encoding ?(E), of which two are adjacent to the genes encoding ChrR-type ..
  9. Creus C, Graziano M, Casanovas E, Pereyra M, Simontacchi M, Puntarulo S, et al. Nitric oxide is involved in the Azospirillum brasilense-induced lateral root formation in tomato. Planta. 2005;221:297-303 pubmed
    ..In this work, NO production by Azospirillum brasilense Sp245 was detected by electron paramagnetic resonance (6.4 nmol...

More Information

Publications83

  1. Herschkovitz Y, Lerner A, Davidov Y, Rothballer M, Hartmann A, Okon Y, et al. Inoculation with the plant-growth-promoting rhizobacterium Azospirillum brasilense causes little disturbance in the rhizosphere and rhizoplane of maize (Zea mays). Microb Ecol. 2005;50:277-88 pubmed
    Inoculation with Azospirillum brasilense exerts beneficial effects on plant growth and crop yields. In this study, a comparative analysis of maize (Zea mays) root inoculated or not inoculated with A...
  2. Ona O, Van Impe J, Prinsen E, Vanderleyden J. Growth and indole-3-acetic acid biosynthesis of Azospirillum brasilense Sp245 is environmentally controlled. FEMS Microbiol Lett. 2005;246:125-32 pubmed
    Batch and fed batch cultures of Azospirillum brasilense Sp245 were conducted in a bioreactor...
  3. Sperotto R, Gross J, Vedoy C, Passaglia L, Schrank I. The electron transfer flavoprotein fixABCX gene products from Azospirillum brasilense show a NifA-dependent promoter regulation. Curr Microbiol. 2004;49:267-73 pubmed publisher
    ..brasilense...
  4. Fedonenko Y, Zatonsky G, Konnova S, Zdorovenko E, Ignatov V. Structure of the O-specific polysaccharide of the lipopolysaccharide of Azospirillum brasilense Sp245. Carbohydr Res. 2002;337:869-72 pubmed
    ..An O-specific polysaccharide was isolated from the lipopolysaccharide of a plant-growth-promoting bacterium Azospirillum brasilense Sp245 and studied by sugar analyses along with one- and two-dimensional 1H and 13C NMR spectroscopy, ..
  5. Ge S, Tao L, Chen S. Expression and functional analysis of aminotransferase involved in indole-3-acetic acid biosynthesis in Azospirillum brasilense Yu62. Biochemistry (Mosc). 2009;74:81-4 pubmed
    In this study, atrC (a novel gene from Azospirillum brasilense identified in our laboratory) was expressed in Escherichia coli, and SDS-PAGE analysis of the expressed AtrC revealed the apparent molecular weight of 45 kD...
  6. Mishra M, Kumar S, Gupta N, Kaur S, Gupta A, Tripathi A. An extracytoplasmic function sigma factor cotranscribed with its cognate anti-sigma factor confers tolerance to NaCl, ethanol and methylene blue in Azospirillum brasilense Sp7. Microbiology. 2011;157:988-99 pubmed publisher
    b>Azospirillum brasilense, a plant-growth-promoting rhizobacterium, is exposed to changes in its abiotic environment, including fluctuations in temperature, salinity, osmolarity, oxygen concentration and nutrient concentration, in the ..
  7. Carrillo A, Li C, Bashan Y. Increased acidification in the rhizosphere of cactus seedlings induced by Azospirillum brasilense. Naturwissenschaften. 2002;89:428-32 pubmed
    ..giant cardon, Pachycereus pringlei) after inoculation with the plant growth-promoting bacterium Azospirillum brasilense Cd, in the presence or absence of ammonium and nitrate, was studied to understand how to increase growth ..
  8. Zakharova E, Shcherbakov A, Brudnik V, Skripko N, Bulkhin N, Ignatov V. Biosynthesis of indole-3-acetic acid in Azospirillum brasilense. Insights from quantum chemistry. Eur J Biochem. 1999;259:572-6 pubmed
    ..was assessed that indole, anthranilic acid, and Trp might be utilized as precursors for IAA synthesis by Azospirillum brasilense strain Sp 245...
  9. Bahat Samet E, Castro Sowinski S, Okon Y. Arabinose content of extracellular polysaccharide plays a role in cell aggregation of Azospirillum brasilense. FEMS Microbiol Lett. 2004;237:195-203 pubmed
    ..produced by wild type strain and several mutants of the plant growth promoting rhizobacterium (PGPR) Azospirillum brasilense. In a fructose synthetic medium, wild type strain Sp7 produced a glucose-rich EPS during exponential ..
  10. Kaur S, Mishra M, Tripathi A. Regulation of expression and biochemical characterization of a beta-class carbonic anhydrase from the plant growth-promoting rhizobacterium, Azospirillum brasilense Sp7. FEMS Microbiol Lett. 2009;299:149-58 pubmed publisher
    ..Genome analysis of Azospirillum brasilense, a nonphotosynthetic, nitrogen-fixing, rhizobacterium, revealed an ORF with homology to beta-class ..
  11. Xie Z, Ulrich L, Zhulin I, Alexandre G. PAS domain containing chemoreceptor couples dynamic changes in metabolism with chemotaxis. Proc Natl Acad Sci U S A. 2010;107:2235-40 pubmed publisher
    ..b>Azospirillum brasilense are motile bacteria that can fix atmospheric nitrogen under microaerophilic conditions...
  12. Arsene F, Kaminski P, Elmerich C. Modulation of NifA activity by PII in Azospirillum brasilense: evidence for a regulatory role of the NifA N-terminal domain. J Bacteriol. 1996;178:4830-8 pubmed
    b>Azospirillum brasilense NifA, which is synthesized under all physiological conditions, exists in an active or inactive from depending on the availability of ammonia...
  13. Lerner A, Castro Sowinski S, Valverde A, Lerner H, Dror R, Okon Y, et al. The Azospirillum brasilense Sp7 noeJ and noeL genes are involved in extracellular polysaccharide biosynthesis. Microbiology. 2009;155:4058-68 pubmed publisher
    b>Azospirillum brasilense is a plant root-colonizing bacterium that exerts beneficial effects on the growth of many agricultural crops...
  14. Couillerot O, Combes Meynet E, Pothier J, Bellvert F, Challita E, Poirier M, et al. The role of the antimicrobial compound 2,4-diacetylphloroglucinol in the impact of biocontrol Pseudomonas fluorescens F113 on Azospirillum brasilense phytostimulators. Microbiology. 2011;157:1694-705 pubmed publisher
    ..Here, the effects of synthetic Phl and Phl(+) Pseudomonas fluorescens F113 on Azospirillum brasilense phytostimulators were investigated. Most A. brasilense strains were moderately sensitive to Phl...
  15. Taylor B, Watts K, Johnson M. Oxygen and redox sensing by two-component systems that regulate behavioral responses: behavioral assays and structural studies of aer using in vivo disulfide cross-linking. Methods Enzymol. 2007;422:190-232 pubmed
    ..These include 5-iodoacetamidofluorescein, 4-acetamido-4-disulfonic acid, disodium salt (AMS), and methoxy polyethylene glycol maleimide, a 5-kDa molecular mass probe that alters the mobility of Aer on SDS-PAGE...
  16. Versées W, Spaepen S, Vanderleyden J, Steyaert J. The crystal structure of phenylpyruvate decarboxylase from Azospirillum brasilense at 1.5 A resolution. Implications for its catalytic and regulatory mechanism. FEBS J. 2007;274:2363-75 pubmed
    Phenylpyruvate decarboxylase (PPDC) of Azospirillum brasilense, involved in the biosynthesis of the plant hormone indole-3-acetic acid and the antimicrobial compound phenylacetic acid, is a thiamine diphosphate-dependent enzyme that ..
  17. Valverde A, Okon Y, Burdman S. cDNA-AFLP reveals differentially expressed genes related to cell aggregation of Azospirillum brasilense. FEMS Microbiol Lett. 2006;265:186-94 pubmed
    The response regulatory gene flcA controls the differentiation process of Azospirillum brasilense from vegetative state to cyst-like forms, both in culture and in association with plants...
  18. Lebsky V, Gonzalez Bashan L, Bashan Y. Ultrastructure of interaction in alginate beads between the microalga Chlorella vulgaris with its natural associative bacterium Phyllobacterium myrsinacearum and with the plant growth-promoting bacterium Azospirillum brasilense. Can J Microbiol. 2001;47:1-8 pubmed
    ..wastewater treatment, was coimmobilized and coincubated either with the plant growth-promoting bacterium Azospirillum brasilense, or with its natural associative bacterium Phyllobacterium myrsinacearum, in alginate beads designed for ..
  19. Vanbleu E, Marchal K, Lambrecht M, Mathys J, Vanderleyden J. Annotation of the pRhico plasmid of Azospirillum brasilense reveals its role in determining the outer surface composition. FEMS Microbiol Lett. 2004;232:165-72 pubmed
    The plant growth-promoting soil bacterium Azospirillum brasilense enhances growth of economically important crops, such as wheat, corn and rice...
  20. Huergo L, Merrick M, Monteiro R, Chubatsu L, Steffens M, Pedrosa F, et al. In vitro interactions between the PII proteins and the nitrogenase regulatory enzymes dinitrogenase reductase ADP-ribosyltransferase (DraT) and dinitrogenase reductase-activating glycohydrolase (DraG) in Azospirillum brasilense. J Biol Chem. 2009;284:6674-82 pubmed publisher
    The activity of the nitrogenase enzyme in the diazotroph Azospirillum brasilense is reversibly inactivated by ammonium through ADP-ribosylation of the nitrogenase NifH subunit...
  21. Kadouri D, Jurkevitch E, Okon Y. Involvement of the reserve material poly-beta-hydroxybutyrate in Azospirillum brasilense stress endurance and root colonization. Appl Environ Microbiol. 2003;69:3244-50 pubmed
    ..b>Azospirillum brasilense strain Sp7 and a phbC (PHB synthase) mutant strain in which PHB production is impaired were evaluated for ..
  22. Fibach Paldi S, Burdman S, Okon Y. Key physiological properties contributing to rhizosphere adaptation and plant growth promotion abilities of Azospirillum brasilense. FEMS Microbiol Lett. 2012;326:99-108 pubmed publisher
    b>Azospirillum brasilense is a plant growth promoting rhizobacterium (PGPR) that is being increasingly used in agriculture in a commercial scale. Recent research has elucidated key properties of A...
  23. Bible A, Stephens B, Ortega D, Xie Z, Alexandre G. Function of a chemotaxis-like signal transduction pathway in modulating motility, cell clumping, and cell length in the alphaproteobacterium Azospirillum brasilense. J Bacteriol. 2008;190:6365-75 pubmed publisher
    ..transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this ..
  24. Kamnev A, Sadovnikova J, Tarantilis P, Polissiou M, Antonyuk L. Responses of Azospirillum brasilense to nitrogen deficiency and to wheat lectin: a diffuse reflectance infrared fourier transform (DRIFT) spectroscopic study. Microb Ecol. 2008;56:615-24 pubmed publisher
    For the rhizobacterium Azospirillum brasilense, the optimal nutritional range of C:N ratios corresponds to the presence of malate (ca. 3 to 5 g l(-1) of its sodium salt) and ammonium (ca. 0...
  25. Spaepen S, Versées W, Gocke D, Pohl M, Steyaert J, Vanderleyden J. Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense. J Bacteriol. 2007;189:7626-33 pubmed
    b>Azospirillum brasilense belongs to the plant growth-promoting rhizobacteria with direct growth promotion through the production of the phytohormone indole-3-acetic acid (IAA)...
  26. Siuti P, Green C, Edwards A, Doktycz M, Alexandre G. The chemotaxis-like Che1 pathway has an indirect role in adhesive cell properties of Azospirillum brasilense. FEMS Microbiol Lett. 2011;323:105-12 pubmed publisher
    The Azospirillum brasilense chemotaxis-like Che1 signal transduction pathway was recently shown to modulate changes in adhesive cell surface properties that, in turn, affect cell-to-cell aggregation and flocculation behaviors rather than ..
  27. Boyko A, Dmitrenok A, Fedonenko Y, Zdorovenko E, Konnova S, Knirel Y, et al. Structural analysis of the O-polysaccharide of the lipopolysaccharide from Azospirillum brasilense Jm6B2 containing 3-O-methyl-D-rhamnose (D-acofriose). Carbohydr Res. 2012;355:92-5 pubmed publisher
    ..lipopolysaccharide isolated by phenol-water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense Jm6B2...
  28. Sigida E, Fedonenko Y, Zdorovenko E, Konnova S, Shashkov A, Ignatov V, et al. Structure of repeating units of a polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense SR80. Carbohydr Res. 2013;371:40-4 pubmed publisher
    ..fraction was obtained by mild acid hydrolysis of the lipopolysaccharide of diazotrophic rhizobacterium Azospirillum brasilense SR80 followed by GPC on Sephadex G-50 Superfine...
  29. Vande Broek A, Gysegom P, Ona O, Hendrickx N, Prinsen E, Van Impe J, et al. Transcriptional analysis of the Azospirillum brasilense indole-3-pyruvate decarboxylase gene and identification of a cis-acting sequence involved in auxin responsive expression. Mol Plant Microbe Interact. 2005;18:311-23 pubmed
    Expression of the Azospirillum brasilense ipdC gene, encoding an indole-3-pyruvate decarboxylase, a key enzyme in the production of indole-3-acetic acid (IAA) in this bacterium, is upregulated by IAA...
  30. Burdman S, Dulguerova G, Okon Y, Jurkevitch E. Purification of the major outer membrane protein of Azospirillum brasilense, its affinity to plant roots, and its involvement in cell aggregation. Mol Plant Microbe Interact. 2001;14:555-61 pubmed
    The major outer membrane protein (MOMP) of the nitrogen-fixing rhizobacterium Azospirillum brasilense strain Cd was purified and isolated by gel filtration, and antiserum against this protein was obtained...
  31. Chen S, Liu L, Zhou X, Elmerich C, Li J. Functional analysis of the GAF domain of NifA in Azospirillum brasilense: effects of Tyr-->Phe mutations on NifA and its interaction with GlnB. Mol Genet Genomics. 2005;273:415-22 pubmed
    Regulation of NifA activity in Azospirillum brasilense depends on GlnB (a PII protein), and it was previously reported that the target of GlnB activity is the N-terminal domain of NifA...
  32. Greer Phillips S, Stephens B, Alexandre G. An energy taxis transducer promotes root colonization by Azospirillum brasilense. J Bacteriol. 2004;186:6595-604 pubmed publisher
    ..In Azospirillum brasilense, energy taxis was shown to be the principal form of locomotor control...
  33. Jofr E, Lagares A, Mori G. Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense. FEMS Microbiol Lett. 2004;231:267-75 pubmed publisher
    The interaction between Azospirillum brasilense and plants is not fully understood, although several bacterial surface components like exopolysaccharides (EPS), flagella, and capsular polysaccharides are required for attachment and ..
  34. Wasim M, Bible A, Xie Z, Alexandre G. Alkyl hydroperoxide reductase has a role in oxidative stress resistance and in modulating changes in cell-surface properties in Azospirillum brasilense Sp245. Microbiology. 2009;155:1192-202 pubmed publisher
    An ahpC mutant derivative of Azospirillum brasilense Sp245 (strain SK586) that encodes an alkyl hydroperoxide reductase was found to be more sensitive to oxidative stress caused by organic hydroperoxides compared with the wild-type...
  35. Van Dommelen A, Spaepen S, Vanderleyden J. Identification of the glutamine synthetase adenylyltransferase of Azospirillum brasilense. Res Microbiol. 2009;160:205-12 pubmed publisher
    ..conserved sequences in glutamine synthetase adenylyltransferases, we amplified part of the glnE gene of Azospirillum brasilense Sp7. The complete glnE sequence of A...
  36. Acosta Cruz E, Wisniewski Dyé F, Rouy Z, Barbe V, Valdés M, Mavingui P. Insights into the 1.59-Mbp largest plasmid of Azospirillum brasilense CBG497. Arch Microbiol. 2012;194:725-36 pubmed publisher
    The plant growth-promoting proteobacterium Azospirillum brasilense enhances growth of many economically important crops, such as wheat, maize, and rice. The sequencing and annotation of the 1.59-Mbp replicon of A...
  37. Huergo L, Souza E, Araujo M, Pedrosa F, Chubatsu L, Steffens M, et al. ADP-ribosylation of dinitrogenase reductase in Azospirillum brasilense is regulated by AmtB-dependent membrane sequestration of DraG. Mol Microbiol. 2006;59:326-37 pubmed
    ..protein AmtB and the cellular localization of DraG in the regulation of the NifH-modification process in Azospirillum brasilense. GlnB, GlnZ and DraG were all membrane-associated after an ammonium shock, and both this membrane ..
  38. Malhotra M, Srivastava S. Targeted engineering of Azospirillum brasilense SM with indole acetamide pathway for indoleacetic acid over-expression. Can J Microbiol. 2006;52:1078-84 pubmed
    ..IAA biosynthesis by a natural isolate of Azospirillum brasilense SM was studied and observed to be tryptophan-inducible and -dependent in nature...
  39. Bible A, Russell M, Alexandre G. The Azospirillum brasilense Che1 chemotaxis pathway controls swimming velocity, which affects transient cell-to-cell clumping. J Bacteriol. 2012;194:3343-55 pubmed publisher
    The Che1 chemotaxis-like pathway of Azospirillum brasilense contributes to chemotaxis and aerotaxis, and it has also been found to contribute to regulating changes in cell surface adhesive properties that affect the propensity of cells ..
  40. Rivarola V, Castro S, Mori G, Jofré E, Fabra A, Garnica R, et al. Response of Azospirillum brasilense Cd to sodium chloride stress. Antonie Van Leeuwenhoek. 1998;73:255-61 pubmed
    Growth of Azospirillum brasilense Cd in the presence of different NaCl concentrations showed that it tolerates up to 200 mM NaCl in the medium, without appreciable decline in growth rate...
  41. Egorenkova I, Konnova S, Fedonenko I, Dykman L, Ignatov V. [The role of polysaccharide-containing components of the Azospirillum brasilense capsule in adsorbing bacteria on wheat seedling roots]. Mikrobiologiia. 2001;70:45-50 pubmed
    b>Azospirillum brasilense cells deprived of capsular exopolysaccharides completely lost their ability to bind wheat germ agglutinin (WGA) and much of their ability to attach to wheat seedling roots...
  42. Zhang Y, Burris R, Ludden P, Roberts G. Presence of a second mechanism for the posttranslational regulation of nitrogenase activity in Azospirillum brasilense in response to ammonium. J Bacteriol. 1996;178:2948-53 pubmed
    ..of dinitrogenase reductase plays a significant role in the regulation of nitrogenase activity in Azospirillum brasilense, it is not the only mechanism of that regulation...
  43. Bianciotto V, Andreotti S, Balestrini R, Bonfante P, Perotto S. Extracellular polysaccharides are involved in the attachment of Azospirillum brasilense and Rhizobium leguminosarum to arbuscular mycorrhizal structures. Eur J Histochem. 2001;45:39-49 pubmed
  44. Hauwaerts D, Alexandre G, Das S, Vanderleyden J, Zhulin I. A major chemotaxis gene cluster in Azospirillum brasilense and relationships between chemotaxis operons in alpha-proteobacteria. FEMS Microbiol Lett. 2002;208:61-7 pubmed
    b>Azospirillum brasilense shows chemotaxis to a variety of nutrients and oxygen. Genes encoding the central signal transduction pathway in chemotaxis were identified by phenotypic complementation of generally non-chemotactic mutants...
  45. Lerner A, Okon Y, Burdman S. The wzm gene located on the pRhico plasmid of Azospirillum brasilense Sp7 is involved in lipopolysaccharide synthesis. Microbiology. 2009;155:791-804 pubmed publisher
    Several genes involved in the interaction between Azospirillum brasilense Sp7 and plants are located on the pRhico plasmid. Here we report the characterization of an Sp7 mutant strain with impairment of the pRhico-located gene wzm...
  46. Kumar S, Kateriya S, Singh V, Tanwar M, Agarwal S, Singh H, et al. Bacteriophytochrome controls carotenoid-independent response to photodynamic stress in a non-photosynthetic rhizobacterium, Azospirillum brasilense Sp7. Sci Rep. 2012;2:872 pubmed publisher
    ..b>Azospirillum brasilense, a non-photosynthetic rhizobacterium, harbours a pair of BphPs out of which AbBphP1 is a homolog of ..
  47. Somers E, Ptacek D, Gysegom P, Srinivasan M, Vanderleyden J. Azospirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis. Appl Environ Microbiol. 2005;71:1803-10 pubmed
    An antimicrobial compound was isolated from Azospirillum brasilense culture extracts by high-performance liquid chromatography and further identified by gas chromatography-mass spectrometry as the auxin-like molecule, phenylacetic acid (..
  48. Perrig D, Boiero M, Masciarelli O, Penna C, Ruiz O, Cassán F, et al. Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense, and implications for inoculant formulation. Appl Microbiol Biotechnol. 2007;75:1143-50 pubmed
    ..biosynthesis, siderophore production, and phosphate solubilization in two strains (Cd and Az39) of Azospirillum brasilense used for inoculant formulation in Argentina during the last 20 years...
  49. Herschkovitz Y, Lerner A, Davidov Y, Okon Y, Jurkevitch E. Azospirillum brasilense does not affect population structure of specific rhizobacterial communities of inoculated maize (Zea mays). Environ Microbiol. 2005;7:1847-52 pubmed
    ..In this study the effect of Azospirillum brasilense inoculation on maize (Zea mays) rhizosphere Actinobacteria, Bacteroidetes, alpha-Proteobacteria, ..
  50. Mora P, Rosconi F, Franco Fraguas L, Castro Sowinski S. Azospirillum brasilense Sp7 produces an outer-membrane lectin that specifically binds to surface-exposed extracellular polysaccharide produced by the bacterium. Arch Microbiol. 2008;189:519-24 pubmed
    ..Results show the specific recognition and binding between EPS and OML. The potential relationship between cell-to-cell aggregation and the OML-EPS interaction is discussed...
  51. Malhotra M, Srivastava S. An ipdC gene knock-out of Azospirillum brasilense strain SM and its implications on indole-3-acetic acid biosynthesis and plant growth promotion. Antonie Van Leeuwenhoek. 2008;93:425-33 pubmed publisher
    ..gene (ipdC), coding for a key enzyme of the indole-3-pyruvic acid pathway of IAA biosynthesis in Azospirillum brasilense SM was functionally disrupted in a site-specific manner...
  52. Thirunavukkarasu N, Mishra M, Spaepen S, Vanderleyden J, Gross C, Tripathi A. An extra-cytoplasmic function sigma factor and anti-sigma factor control carotenoid biosynthesis in Azospirillum brasilense. Microbiology. 2008;154:2096-105 pubmed publisher
    Strains Sp7 and Cd of Azospirillum brasilense, a plant growth-promoting rhizobacterium, differ in synthesis of carotenoids...
  53. Stephens B, Loar S, Alexandre G. Role of CheB and CheR in the complex chemotactic and aerotactic pathway of Azospirillum brasilense. J Bacteriol. 2006;188:4759-68 pubmed
    It has previously been reported that the alpha-proteobacterium Azospirillum brasilense undergoes methylation-independent chemotaxis; however, a recent study revealed cheB and cheR genes in this organism...
  54. Huergo L, Chubatsu L, Souza E, Pedrosa F, Steffens M, Merrick M. Interactions between PII proteins and the nitrogenase regulatory enzymes DraT and DraG in Azospirillum brasilense. FEBS Lett. 2006;580:5232-6 pubmed
    In Azospirillum brasilense ADP-ribosylation of dinitrogenase reductase (NifH) occurs in response to addition of ammonium to the extracellular medium and is mediated by dinitrogenase reductase ADP-ribosyltransferase (DraT) and reversed by ..
  55. Jha J, Sinha S, Maiti M, Basu A, Mukhopadhyay U, Sen S. Functional expression of an acyl carrier protein (ACP) from Azospirillum brasilense alters fatty acid profiles in Escherichia coli and Brassica juncea. Plant Physiol Biochem. 2007;45:490-500 pubmed
    ..is characteristically different from the plant's counterpart, we explored the property of an ACP from Azospirillum brasilense (Ab), a plant-associative aerobic bacterium, to find its role in changing the fatty acid profile in ..
  56. Baudoin E, Lerner A, Mirza M, El Zemrany H, Prigent Combaret C, Jurkevich E, et al. Effects of Azospirillum brasilense with genetically modified auxin biosynthesis gene ipdC upon the diversity of the indigenous microbiota of the wheat rhizosphere. Res Microbiol. 2010;161:219-26 pubmed publisher
    ..Here, wheat seeds were inoculated with the wild-type strain Azospirillum brasilense Sp245 or one of three genetically modified (GM) derivatives and grown for one month...
  57. Ishida M, Assumpção M, Machado H, Benelli E, Souza E, Pedrosa F. Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense. Braz J Med Biol Res. 2002;35:651-61 pubmed
    Two Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A...
  58. de Bashan L, Bashan Y, Moreno M, Lebsky V, Bustillos J. Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense. Can J Microbiol. 2002;48:514-21 pubmed
    ..sorokiniana co-immobilized separately in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense Cd, resulted in significant changes in microalgal-population size, cell size, cell cytology, pigment, ..
  59. Araujo L, Monteiro R, Souza E, Steffens M, Rigo L, Pedrosa F, et al. GlnB is specifically required for Azospirillum brasilense NifA activity in Escherichia coli. Res Microbiol. 2004;155:491-5 pubmed
    The Azospirillum brasilense transcription regulator NifA and the nitrogen-status signaling proteins GlnB, GlnZ and GlnK were expressed in Escherichia coli and analyzed for their ability to activate nif gene expression...
  60. Van Dommelen A, Keijers V, Wollebrants A, Vanderleyden J. Phenotypic changes resulting from distinct point mutations in the Azospirillum brasilense glnA gene, encoding glutamine synthetase. Appl Environ Microbiol. 2003;69:5699-701 pubmed
    Sequencing the glnA genes of two chemically induced Azospirillum brasilense glutamine synthetase mutants revealed an Arg-->Cys mutation, corresponding to the glutamate binding site, in one mutant and an Asp-->Asn mutation, ..
  61. Carreño López R, Sánchez A, Camargo N, Elmerich C, Baca B. Characterization of chsA, a new gene controlling the chemotactic response in Azospirillum brasilense Sp7. Arch Microbiol. 2009;191:501-7 pubmed publisher
    We report, here, the characterization of a mutant strain of Azospirillum brasilense Sp7 impaired in surface motility and chemotactic response...
  62. Petoukhov M, Svergun D, Konarev P, Ravasio S, van den Heuvel R, Curti B, et al. Quaternary structure of Azospirillum brasilense NADPH-dependent glutamate synthase in solution as revealed by synchrotron radiation x-ray scattering. J Biol Chem. 2003;278:29933-9 pubmed
    b>Azospirillum brasilense glutamate synthase (GltS) is the prototype of bacterial NADPH-dependent enzymes, a class of complex iron-sulfur flavoproteins essential in ammonia assimilation processes...
  63. Rodríguez Salazar J, SUAREZ R, Caballero Mellado J, Iturriaga G. Trehalose accumulation in Azospirillum brasilense improves drought tolerance and biomass in maize plants. FEMS Microbiol Lett. 2009;296:52-9 pubmed publisher
    ..In this work the effect of inoculating maize plants with genetically engineered Azospirillum brasilense for trehalose biosynthesis was determined...
  64. Van Puyvelde S, Cloots L, Engelen K, Das F, Marchal K, Vanderleyden J, et al. Transcriptome analysis of the rhizosphere bacterium Azospirillum brasilense reveals an extensive auxin response. Microb Ecol. 2011;61:723-8 pubmed publisher
    The rhizosphere bacterium Azospirillum brasilense produces the auxin indole-3-acetic acid (IAA) through the indole-3-pyruvate pathway...
  65. Boyer M, Haurat J, Samain S, Segurens B, Gavory F, Gonzalez V, et al. Bacteriophage prevalence in the genus Azospirillum and analysis of the first genome sequence of an Azospirillum brasilense integrative phage. Appl Environ Microbiol. 2008;74:861-74 pubmed
    ..brasilense Cd, and a region of 27.3 kb of phiAb-Cd was found to be duplicated on the 130-kb pRhico plasmid previously sequenced from A. brasilense Sp7, the parental strain of A. brasilense Cd...
  66. Nishikawa C, Araujo L, Kadowaki M, Monteiro R, Steffens M, Pedrosa F, et al. Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense. Braz J Med Biol Res. 2012;45:113-7 pubmed
    b>Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize...
  67. Kadouri D, Jurkevitch E, Okon Y. Poly beta-hydroxybutyrate depolymerase (PhaZ) in Azospirillum brasilense and characterization of a phaZ mutant. Arch Microbiol. 2003;180:309-18 pubmed
    ..PHB depolymerase (PhaZ) is an essential enzyme in PHB degradation. The phaZ gene was identified in Azospirillum brasilense, cloned, sequenced, and shown to be located on the chromosome...
  68. Pereg Gerk L, Gilchrist K, Kennedy I. Mutants with enhanced nitrogenase activity in hydroponic Azospirillum brasilense-wheat associations. Appl Environ Microbiol. 2000;66:2175-84 pubmed
    ..flocculation, differentiation into cyst-like forms, and root colonization on nitrogenase expression by Azospirillum brasilense is described...
  69. de Zamaroczy M. Structural homologues P(II) and P(Z) of Azospirillum brasilense provide intracellular signalling for selective regulation of various nitrogen-dependent functions. Mol Microbiol. 1998;29:449-63 pubmed
    P(II) (glnB) is a signal transduction protein that in Azospirillum brasilense is specifically required for nitrogen fixation...
  70. Konnova O, Burygin G, Fedonenko I, Matora L, Pankin K, Konnova S, et al. [Chemical composition and immunochemical characteristics of the lipopolysaccharide of nitrogen-fixing rhizobacterium Azospirillum brasilense CD]. Mikrobiologiia. 2006;75:383-8 pubmed
    The chemical composition of the lipopolysaccharide of the associative diazotrophic rhizobacterium Azospirillum brasilense Cd has been studied...
  71. Lerner A, Castro Sowinski S, Lerner H, Okon Y, Burdman S. Glycogen phosphorylase is involved in stress endurance and biofilm formation in Azospirillum brasilense Sp7. FEMS Microbiol Lett. 2009;300:75-82 pubmed publisher
    ..the identification of a glycogen phosphorylase (glgP) gene in the plant growth-promoting rhizobacterium Azospirillum brasilense, Sp7, and the characterization of a glgP marker exchange mutant of this strain...
  72. Kochar M, Srivastava S. Surface colonization by Azospirillum brasilense SM in the indole-3-acetic acid dependent growth improvement of sorghum. J Basic Microbiol. 2012;52:123-31 pubmed publisher
    ..The results presented here confirm Azospirillum brasilense strain SM as a competent plant growth promoting bacterium over mid- and long-term associations with ..
  73. Araujo L, Huergo L, Invitti A, Gimenes C, Bonatto A, Monteiro R, et al. Different responses of the GlnB and GlnZ proteins upon in vitro uridylylation by the Azospirillum brasilense GlnD protein. Braz J Med Biol Res. 2008;41:289-94 pubmed
    b>Azospirillum brasilense is a diazotroph found in association with important agricultural crops...
  74. Alahari A, Tripathi A, Le Rudulier D. Cloning and characterization of a fur homologue from Azospirillum brasilense Sp7. Curr Microbiol. 2006;52:123-7 pubmed
    A homologue of the ferric uptake regulator gene, fur, was identified from a Azospirillum brasilense Sp7 genomic DNA clone. Experiments performed with transcriptional lacZ fusions demonstrated that the A...