rhizobium leguminosarum

Summary

Summary: A species of gram-negative, aerobic bacteria that is found in soil and which causes formation of root nodules on some, but not all, types of field pea, lentil, kidney bean, and clover.

Top Publications

  1. George R, Kelly S, Price N, Erbse A, Fisher M, Lund P. Three GroEL homologues from Rhizobium leguminosarum have distinct in vitro properties. Biochem Biophys Res Commun. 2004;324:822-8 pubmed
    ..Most but not all bacteria express single GroEL and GroES proteins. Rhizobium leguminosarum strain A34 encodes three complete operons encoding homologues to GroEL and GroES...
  2. Lodwig E, Kumar S, Allaway D, Bourdes A, Prell J, Priefer U, et al. Regulation of L-alanine dehydrogenase in Rhizobium leguminosarum bv. viciae and its role in pea nodules. J Bacteriol. 2004;186:842-9 pubmed
    ..We propose that the role of AldA may be to balance the alanine level for optimal functioning of bacteroid metabolism rather than to synthesize alanine as the sole product of N(2) reduction. ..
  3. Patriarca E, Chiurazzi M, Manco G, Riccio A, Lamberti A, De Paolis A, et al. Activation of the Rhizobium leguminosarum glnII gene by NtrC is dependent on upstream DNA sequences. Mol Gen Genet. 1992;234:337-45 pubmed
    The cloning and sequence determination is reported of the DNA region of Rhizobium leguminosarum coding for glutamine synthetase II (GSII)...
  4. Bloemberg G, Thomas Oates J, Lugtenberg B, Spaink H. Nodulation protein NodL of Rhizobium leguminosarum O-acetylates lipo-oligosaccharides, chitin fragments and N-acetylglucosamine in vitro. Mol Microbiol. 1994;11:793-804 pubmed
    ..All lipo-oligosaccharides identified from Rhizobium leguminosarum bv...
  5. Chabot R, Antoun H, Kloepper J, Beauchamp C. Root colonization of maize and lettuce by bioluminescent Rhizobium leguminosarum biovar phaseoli. Appl Environ Microbiol. 1996;62:2767-72 pubmed
    Two strains of Rhizobium leguminosarum bv. phaseoli and three other plant growth-promoting rhizobacteria (PGPR) were examined for the potential of maize and lettuce root colonization...
  6. Kanipes M, Ribeiro A, Lin S, Cotter R, Raetz C. A mannosyl transferase required for lipopolysaccharide inner core assembly in Rhizobium leguminosarum. Purification, substrate specificity, and expression in Salmonella waaC mutants. J Biol Chem. 2003;278:16356-64 pubmed
    ..We now report the purification and characterization of the Rhizobium leguminosarum mannosyl transferase LpcC, which adds a mannose unit to the inner 3-deoxy-d-manno-octulosonic acid (Kdo) ..
  7. Ivashina T, Khmelnitsky M, Shlyapnikov M, Kanapin A, Ksenzenko V. The pss4 gene from Rhizobium leguminosarum by viciae VF39: cloning, sequence and the possible role in polysaccharide production and nodule formation. Gene. 1994;150:111-6 pubmed
    The Tn5 insertion into the genome of Rhizobium leguminosarum bv viciae VF39, resulting in non-mucoid growth and formation of non-N2-fixing nodule-like structures on Vicia faba plants, was mapped within a 1.4-kb EcoRV-SacI fragment...
  8. Pérez Segura G, Pérez Oseguera Á, Cevallos M. The repAC replication system of the Rhizobium leguminosarum pRL7 plasmid is functional: implications regarding the origin and evolution of repABC plasmids. Plasmid. 2013;69:49-57 pubmed publisher
    ..evolutionary consequences of one of these atypical gene associations: the repAC genes present in the Rhizobium leguminosarum plasmid pRL7...
  9. Mazur A, Krol J, Wielbo J, Urbanik Sypniewska T, Skorupska A. Rhizobium leguminosarum bv. trifolii PssP protein is required for exopolysaccharide biosynthesis and polymerization. Mol Plant Microbe Interact. 2002;15:388-97 pubmed
    b>Rhizobium leguminosarum bv. trifolii produces an acidic exopolysaccharide (EPS) that is important for the induction of nitrogen-fixing nodules on clover...

More Information

Publications143 found, 100 shown here

  1. Laguerre G, Depret G, Bourion V, Duc G. Rhizobium leguminosarum bv. viciae genotypes interact with pea plants in developmental responses of nodules, roots and shoots. New Phytol. 2007;176:680-90 pubmed
    ..of pea (Pisum sativum) was studied in relation to the genetic diversity of their nitrogen-fixing symbiont Rhizobium leguminosarum bv. viciae...
  2. Kutkowska J, Turska Szewczuk A, Janczarek M, Paduch R, Kamińska T, Urbanik Sypniewska T. Biological activity of (lipo)polysaccharides of the exopolysaccharide-deficient mutant Rt120 derived from Rhizobium leguminosarum bv. trifolii strain TA1. Biochemistry (Mosc). 2011;76:840-50 pubmed publisher
    Lipopolysaccharides (LPS) from Rhizobium leguminosarum biovar trifolii TA1 (RtTA1) and its mutant Rt120 in the pssB?pssA intergenic region as well as degraded polysaccharides (DPS) derived from the LPS were elucidated in terms of their ..
  3. Pollock T, van Workum W, Thorne L, Mikolajczak M, Yamazaki M, Kijne J, et al. Assignment of biochemical functions to glycosyl transferase genes which are essential for biosynthesis of exopolysaccharides in Sphingomonas strain S88 and Rhizobium leguminosarum. J Bacteriol. 1998;180:586-93 pubmed
    ..The spsK gene of Sphingomonas strain S88 and the pssDE genes of Rhizobium leguminosarum were identified as encoding glucuronosyl-(B1-->4)-glucosyl transferases based on reciprocal genetic ..
  4. Mazur A, Stasiak G, Wielbo J, Koper P, Kubik Komar A, Skorupska A. Phenotype profiling of Rhizobium leguminosarum bv. trifolii clover nodule isolates reveal their both versatile and specialized metabolic capabilities. Arch Microbiol. 2013;195:255-67 pubmed publisher
    b>Rhizobium leguminosarum bv. trifolii (Rlt) are soil bacteria inducing nodules on clover, where they fix nitrogen...
  5. Yost C, Rochepeau P, Hynes M. Rhizobium leguminosarum contains a group of genes that appear to code for methyl-accepting chemotaxis proteins. Microbiology. 1998;144 ( Pt 7):1945-56 pubmed
    ..the conserved signalling domain of MCPs by PCR were used to identify potential MCP-encoding genes in Rhizobium leguminosarum. Using a PCR-derived probe created from these primers a genomic library of R...
  6. Brito B, Palacios J, Imperial J, Ruiz Argüeso T. Engineering the Rhizobium leguminosarum bv. viciae hydrogenase system for expression in free-living microaerobic cells and increased symbiotic hydrogenase activity. Appl Environ Microbiol. 2002;68:2461-7 pubmed
    b>Rhizobium leguminosarum bv. viciae UPM791 induces hydrogenase activity in pea (Pisum sativum L.) bacteroids but not in free-living cells...
  7. Frederix M, Edwards A, McAnulla C, Downie J. Co-ordination of quorum-sensing regulation in Rhizobium leguminosarum by induction of an anti-repressor. Mol Microbiol. 2011;81:994-1007 pubmed publisher
    Analysis of quorum-sensing (QS) regulation in Rhizobium leguminosarum revealed an unusual type of gene regulation that relies on the population density-dependent accumulation of an anti-repressor...
  8. Yeoman K, Delgado M, Wexler M, Downie J, Johnston A. High affinity iron acquisition in Rhizobium leguminosarum requires the cycHJKL operon and the feuPQ gene products, which belong to the family of two-component transcriptional regulators. Microbiology. 1997;143 ( Pt 1):127-34 pubmed
    The cycHJKL operon of Rhizobium leguminosarum has previously been shown to be involved in the maturation of cytochrome c, possibly by its involvement in the covalent attachment of haem to the apoprotein...
  9. Clark S, Oresnik I, Hynes M. RpoN of Rhizobium leguminosarum bv. viciae strain VF39SM plays a central role in FnrN-dependent microaerobic regulation of genes involved in nitrogen fixation. Mol Gen Genet. 2001;264:623-33 pubmed
    The rpoN gene, which codes for the alternative transcription factor sigma54, was cloned and sequenced from Rhizobium leguminosarum strain VF39SM...
  10. Spaink H, Wijfjes A, van der Drift K, Haverkamp J, Thomas Oates J, Lugtenberg B. Structural identification of metabolites produced by the NodB and NodC proteins of Rhizobium leguminosarum. Mol Microbiol. 1994;13:821-31 pubmed
    ..On the basis of these and other recent results, we propose that NodB is a chitin oligosaccharide deacetylase, NodC an N-acetylglucosaminyltransferase and, by default, NodA is involved in lipid attachment. ..
  11. Lewthwaite J, George R, Lund P, Poole S, Tormay P, Sharp L, et al. Rhizobium leguminosarum chaperonin 60.3, but not chaperonin 60.1, induces cytokine production by human monocytes: activity is dependent on interaction with cell surface CD14. Cell Stress Chaperones. 2002;7:130-6 pubmed
    ..examined 2 of the 3 chaperonin 60 (Cpn 60) gene products of the nonpathogenic plant symbiotic bacterium, Rhizobium leguminosarum, for their capacity to induce the production of pro- and antiinflammatory cytokines by human cells...
  12. Janczarek M, Skorupska A. The Rhizobium leguminosarum bv. trifolii RosR: transcriptional regulator involved in exopolysaccharide production. Mol Plant Microbe Interact. 2007;20:867-81 pubmed
    ..exopolysaccharide is required for the establishment of symbiosis between the nitrogen-fixing bacterium Rhizobium leguminosarum bv. trifolii and clover. Here, we describe RosR protein from R. leguminosarum bv. trifolii 24...
  13. Ivashina T, Fedorova E, Ashina N, Kalinchuk N, Druzhinina T, Shashkov A, et al. Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis. J Appl Microbiol. 2010;109:731-42 pubmed publisher
    ..g. pyruvylation, plays any role in the development of Rhizobium leguminosarum/Pisum sativum symbiosis...
  14. Wielbo J, Mazur A, Król J, Marczak M, Kutkowska J, Skorupska A. Complexity of phenotypes and symbiotic behaviour of Rhizobium leguminosarum biovar trifolii exopolysaccharide mutants. Arch Microbiol. 2004;182:331-6 pubmed
    b>Rhizobium leguminosarum biovar trifolii strain TA1 polysaccharide synthesis (pss) mutants in the pssD, pssP, pssT and pssO genes and altered in exopolysaccharide (EPS) synthesis were investigated...
  15. Kanjilal Kolar S, Raetz C. Dodecaprenyl phosphate-galacturonic acid as a donor substrate for lipopolysaccharide core glycosylation in Rhizobium leguminosarum. J Biol Chem. 2006;281:12879-87 pubmed
    The lipid A and inner core regions of Rhizobium leguminosarum lipopolysaccharide contain four galacturonic acid (GalA) residues...
  16. Gould P, Maguire M, Lund P. Distinct mechanisms regulate expression of the two major groEL homologues in Rhizobium leguminosarum. Arch Microbiol. 2007;187:1-14 pubmed
    ..2 expression. Inactivation of RpoH led to a reduction in growth rate which could be partly compensated for by inactivation of HrcA, indicating an overlap in the in vivo function of the proteins regulated by these two systems. ..
  17. Williams A, Wilkinson A, Krehenbrink M, Russo D, Zorreguieta A, Downie J. Glucomannan-mediated attachment of Rhizobium leguminosarum to pea root hairs is required for competitive nodule infection. J Bacteriol. 2008;190:4706-15 pubmed publisher
    The Rhizobium leguminosarum biovar viciae genome contains several genes predicted to determine surface polysaccharides. Mutants predicted to affect the initial steps of polysaccharide synthesis were identified and characterized...
  18. Robledo M, Jiménez Zurdo J, Velazquez E, Trujillo M, Zurdo Piñeiro J, Ramírez Bahena M, et al. Rhizobium cellulase CelC2 is essential for primary symbiotic infection of legume host roots. Proc Natl Acad Sci U S A. 2008;105:7064-9 pubmed publisher
    ..biological activity, and symbiotic function of a cell-bound bacterial cellulase (CelC2) enzyme from Rhizobium leguminosarum bv. trifolii, the clover-nodulating endosymbiont...
  19. Michiels J, Vanderleyden J. Cloning and sequence of the Rhizobium leguminosarum biovar phaseoli fixA gene. Biochim Biophys Acta. 1993;1144:232-3 pubmed
    We report the identification and cloning of Rhizobium leguminosarum biovar phaseoli fixABCX homologous genes and the complete nucleotide sequence of the fixA gene...
  20. Figueira E, Lima A, Pereira S. Cadmium tolerance plasticity in Rhizobium leguminosarum bv. viciae: glutathione as a detoxifying agent. Can J Microbiol. 2005;51:7-14 pubmed
    b>Rhizobium leguminosarum bv. viciae strains expressing different degrees of tolerance to metal stress were used in this work to study the basic mechanisms underlying heavy metal tolerance...
  21. Gould P, Burgar H, Lund P. Homologous cpn60 genes in Rhizobium leguminosarum are not functionally equivalent. Cell Stress Chaperones. 2007;12:123-31 pubmed
    ..The Rhizobium leguminosarum strain A34 has 3 complete groE operons, which we have named cpn.1, cpn.2 and cpn.3...
  22. Untiet V, Karunakaran R, Kramer M, Poole P, Priefer U, Prell J. ABC transport is inactivated by the PTS(Ntr) under potassium limitation in Rhizobium leguminosarum 3841. PLoS ONE. 2013;8:e64682 pubmed publisher
    ..growth phenotypes, dry colony morphology and a posttranslational inactivation of ABC transporters in Rhizobium leguminosarum 3841. The PTS(Ntr) proteins EI(Ntr) and 2 copies of EIIA(Ntr) have been described previously...
  23. Li Y, Wexler M, Richardson D, Bond P, Johnston A. Screening a wide host-range, waste-water metagenomic library in tryptophan auxotrophs of Rhizobium leguminosarum and of Escherichia coli reveals different classes of cloned trp genes. Environ Microbiol. 2005;7:1927-36 pubmed
    ..was screened for clones that could correct defined tryptophan auxotrophs of the alpha-proteobacterium Rhizobium leguminosarum and of Escherichia coli...
  24. Schlaman H, Olsthoorn M, Harteveld M, Dörner L, Djordjevic M, Thomas Oates J, et al. The production of species-specific highly unsaturated fatty acyl-containing LCOs from Rhizobium leguminosarum bv. trifolii is stringently regulated by nodD and involves the nodRL genes. Mol Plant Microbe Interact. 2006;19:215-26 pubmed
    A proportion of the Nod factors of some Rhizobium leguminosarum bv...
  25. Janczarek M, Kutkowska J, Piersiak T, Skorupska A. Rhizobium leguminosarum bv. trifolii rosR is required for interaction with clover, biofilm formation and adaptation to the environment. BMC Microbiol. 2010;10:284 pubmed publisher
    b>Rhizobium leguminosarum bv. trifolii is a symbiotic nitrogen-fixing bacterium that elicits nodules on roots of host plants Trifolium spp...
  26. Patriarca E, Riccio A, Tate R, Colonna Romano S, Iaccarino M, Defez R. The ntrBC genes of Rhizobium leguminosarum are part of a complex operon subject to negative regulation. Mol Microbiol. 1993;9:569-77 pubmed
    We report here that ntrB and ntrC genes of Rhizobium leguminosarum biovar phaseoli are cotranscribed with an open reading frame (called ORF1) of unknown function...
  27. Chen X, Feng J, Hou B, Li F, Li Q, Hong G. Modulating DNA bending affects NodD-mediated transcriptional control in Rhizobium leguminosarum. Nucleic Acids Res. 2005;33:2540-8 pubmed
    b>Rhizobium leguminosarum NodD binds to the nod box of the inducible nod gene nodA as a V-shaped tetramer and bends the nod box...
  28. Ding H, Yip C, Hynes M. Genetic characterization of a novel rhizobial plasmid conjugation system in Rhizobium leguminosarum bv. viciae strain VF39SM. J Bacteriol. 2013;195:328-39 pubmed publisher
    b>Rhizobium leguminosarum strain VF39SM contains two plasmids that have previously been shown to be self-transmissible by conjugation...
  29. Janczarek M, Skorupska A. Exopolysaccharide synthesis in Rhizobium leguminosarum bv. trifolii is related to various metabolic pathways. Res Microbiol. 2003;154:433-42 pubmed
    b>Rhizobium leguminosarum bv. trifolii synthesizes extracellular polysaccharide (EPS) that is postulated to be a biologically active signalling molecule in clover symbiosis...
  30. Mazur A, Marczak M, Krol J, Skorupska A. Topological and transcriptional analysis of pssL gene product: a putative Wzx-like exopolysaccharide translocase in Rhizobium leguminosarum bv. trifolii TA1. Arch Microbiol. 2005;184:1-10 pubmed
    ..pssT, pssN and pssP genes, encoding for a protein engaged in polysaccharide polymerization and export in Rhizobium leguminosarum bv. trifolii strain TA1 (RtTA1)...
  31. Morris A, Djordjevic M. The Rhizobium leguminosarum biovar trifolii ANU794 induces novel developmental responses on the subterranean clover cultivar Woogenellup. Mol Plant Microbe Interact. 2006;19:471-9 pubmed
    The clover-nodulating Rhizobium leguminosarum bv. trifolii ANU794 initiates normal root-nodule development with abnormally low efficiency on the Trifolium subterraneum cv. Woogenellup...
  32. Danino V, Wilkinson A, Edwards A, Downie J. Recipient-induced transfer of the symbiotic plasmid pRL1JI in Rhizobium leguminosarum bv. viciae is regulated by a quorum-sensing relay. Mol Microbiol. 2003;50:511-25 pubmed
    Analysis of the regulation of plasmid transfer genes on the symbiotic plasmid pRL1JI in Rhizobium leguminosarum bv...
  33. Karbarz M, Six D, Raetz C. Purification and characterization of the lipid A 1-phosphatase LpxE of Rhizobium leguminosarum. J Biol Chem. 2009;284:414-25 pubmed publisher
    LpxE, a membrane-bound phosphatase found in Rhizobium leguminosarum and some other Gram-negative bacteria, selectively dephosphorylates the 1-position of lipid A on the outer surface of the inner membrane...
  34. Tambalo D, Yost C, Hynes M. Characterization of swarming motility in Rhizobium leguminosarum bv. viciae. FEMS Microbiol Lett. 2010;307:165-74 pubmed publisher
    We have characterized swarming motility in Rhizobium leguminosarum strains 3841 and VF39SM...
  35. Krehenbrink M, Downie J. Identification of protein secretion systems and novel secreted proteins in Rhizobium leguminosarum bv. viciae. BMC Genomics. 2008;9:55 pubmed publisher
    ..The aim of this study was to systematically analyse protein secretion in the recently sequenced strain Rhizobium leguminosarum bv. viciae 3841...
  36. Cieśla J, Bieganowski A, Janczarek M, Urbanik Sypniewska T. Determination of the electrokinetic potential of Rhizobium leguminosarum bv trifolii Rt24.2 using Laser Doppler Velocimetry--a methodological study. J Microbiol Methods. 2011;85:199-205 pubmed publisher
    ..This work presents the methodological aspects of zeta potential determination in strain Rt24.2 of Rhizobium leguminosarum using Laser Doppler Velocimetry combined with Phase Analysis Light Scattering and changed electric field ..
  37. Ding H, Yip C, Geddes B, Oresnik I, Hynes M. Glycerol utilization by Rhizobium leguminosarum requires an ABC transporter and affects competition for nodulation. Microbiology. 2012;158:1369-78 pubmed publisher
    Plasmid curing has shown that the ability to use glycerol as a carbon source is plasmid-encoded in Rhizobium leguminosarum. We isolated the locus responsible for glycerol utilization from plasmid pRleVF39c in R. leguminosarum bv...
  38. Zeze A, Mutch L, Young J. Direct amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil. Environ Microbiol. 2001;3:363-70 pubmed
    ..The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum biovar viciae and Sinorhizobium meliloti...
  39. Todd J, Wexler M, Sawers G, Yeoman K, Poole P, Johnston A. RirA, an iron-responsive regulator in the symbiotic bacterium Rhizobium leguminosarum. Microbiology. 2002;148:4059-71 pubmed
    Mutations in a Rhizobium leguminosarum gene, rirA (rhizobial iron regulator), caused high-level, constitutive expression of at least eight operons whose transcription is normally Fe-responsive and whose products are involved in the ..
  40. Laguerre G, Mavingui P, Allard M, Charnay M, Louvrier P, Mazurier S, et al. Typing of rhizobia by PCR DNA fingerprinting and PCR-restriction fragment length polymorphism analysis of chromosomal and symbiotic gene regions: application to Rhizobium leguminosarum and its different biovars. Appl Environ Microbiol. 1996;62:2029-36 pubmed
    Characterization of 43 strains of Rhizobium leguminosarum biovars viciae, trifolii, and phaseoli was performed by two methodologies based on PCR amplification, i.e...
  41. Richardson J, Oresnik I. L-Rhamnose transport is sugar kinase (RhaK) dependent in Rhizobium leguminosarum bv. trifolii. J Bacteriol. 2007;189:8437-46 pubmed
    Strains of Rhizobium leguminosarum which are unable to catabolize l-rhamnose, a methyl-pentose sugar, are compromised in the ability to compete for nodule occupancy versus wild-type strains...
  42. Mishra R, Singh R, Jaiswal H, Kumar V, Maurya S. Rhizobium-mediated induction of phenolics and plant growth promotion in rice (Oryza sativa L.). Curr Microbiol. 2006;52:383-9 pubmed
    ..different parts (leaf, stem, and root) of rice plants after inoculation with two rhizobial strains, RRE6 (Rhizobium leguminosarum bv. phaseoli) and ANU 843 (R. leguminosarum bv. trifolii) and infection by Rhizoctonia solani...
  43. García Fraile P, Mulas García D, Peix A, Rivas R, González Andrés F, Velázquez E. Phaseolus vulgaris is nodulated in northern Spain by Rhizobium leguminosarum strains harboring two nodC alleles present in American Rhizobium etli strains: biogeographical and evolutionary implications. Can J Microbiol. 2010;56:657-66 pubmed publisher
    ..and recA, and the 16S-23S intergenic spacer showed that all isolates belong to the phylogenetic group of Rhizobium leguminosarum and some of them were identical to those of strains nodulating Vicia or Trifolium...
  44. Foreman D, Vanderlinde E, Bay D, Yost C. Characterization of a gene family of outer membrane proteins (ropB) in Rhizobium leguminosarum bv. viciae VF39SM and the role of the sensor kinase ChvG in their regulation. J Bacteriol. 2010;192:975-83 pubmed publisher
    ..RopB is a previously identified outer membrane protein from Rhizobium leguminosarum bv. viciae that is present in free-living cells but absent in bacteroids (H. P. Roest, I. H. Mulders, C...
  45. Tambalo D, Del Bel K, Bustard D, Greenwood P, Steedman A, Hynes M. Regulation of flagellar, motility and chemotaxis genes in Rhizobium leguminosarum by the VisN/R-Rem cascade. Microbiology. 2010;156:1673-85 pubmed publisher
    ..regulatory roles of VisN, VisR and Rem in the expression of flagellar, motility and chemotaxis genes in Rhizobium leguminosarum biovar viciae strains VF39SM and 3841...
  46. Vanderlinde E, Yost C. Mutation of the sensor kinase chvG in Rhizobium leguminosarum negatively impacts cellular metabolism, outer membrane stability, and symbiosis. J Bacteriol. 2012;194:768-77 pubmed publisher
    ..In the legume symbiont Rhizobium leguminosarum biovar viciae VF39, mutation of chvG, a histidine kinase, caused a number of pleiotropic phenotypes...
  47. Mateos P, Baker D, Petersen M, Velazquez E, Jiménez Zurdo J, Martinez Molina E, et al. Erosion of root epidermal cell walls by Rhizobium polysaccharide-degrading enzymes as related to primary host infection in the Rhizobium-legume symbiosis. Can J Microbiol. 2001;47:475-87 pubmed
    ..The results suggest a complementary role of rhizobial cell-bound glycanases and chitolipooligosaccharides in creating the localized portals of entry for successful primary host infection. ..
  48. Prell J, Boesten B, Poole P, Priefer U. The Rhizobium leguminosarum bv. viciae VF39 gamma-aminobutyrate (GABA) aminotransferase gene (gabT) is induced by GABA and highly expressed in bacteroids. Microbiology. 2002;148:615-23 pubmed
    A Rhizobium leguminosarum bv. viciae VF39 gene (gabT) encoding a gamma-aminobutyrate (GABA) aminotransferase was identified, cloned and characterized...
  49. Król J, Mazur A, Marczak M, Skorupska A. Application of physical and genetic map of Rhizobium leguminosarum bv. trifolii TA1 to comparison of three closely related rhizobial genomes. Mol Genet Genomics. 2008;279:107-21 pubmed
    A combined physical and genetic map of Rhizobium leguminosarum biovar trifolii TA1 (RtTA1) genome was constructed and used in comparison of chromosomal organization with the closely related R. leguminosarum bv...
  50. Ramachandran V, East A, Karunakaran R, Downie J, Poole P. Adaptation of Rhizobium leguminosarum to pea, alfalfa and sugar beet rhizospheres investigated by comparative transcriptomics. Genome Biol. 2011;12:R106 pubmed publisher
    ..b>Rhizobium leguminosarum biovar viciae was grown in the rhizospheres of pea (its legume nodulation host), alfalfa (a non-host ..
  51. Buendia A, Enenkel B, K plin R, Niehaus K, Arnold W, P hler A. The Rhizobium meliloti exoZl exoB fragment of megaplasmid 2: ExoB functions as a UDP-glucose 4-epimerase and ExoZ shows homology to NodX of Rhizobium leguminosarum biovar viciae strain TOM. Mol Microbiol. 1991;5:1519-30 pubmed
    ..In R. meliloti, exoB codes for a UDP-glucose 4-epimerase. A deficiency in the activity of this enzyme fully accounts for all the multiple carbohydrate defects that have been observed in exoB mutants...
  52. Sánchez Andújar B, Coronado C, Philip Hollingsworth S, Dazzo F, Palomares A. Structure and role in symbiosis of the exoB gene of Rhizobium leguminosarum bv trifolii. Mol Gen Genet. 1997;255:131-40 pubmed
    The Rhizobium leguminosarum bv trifolii exoB gene has been isolated by heterologous complementation of an exoB mutant of R. meliloti. We have cloned a chromosomal DNA fragment from the R...
  53. Weir B, Turner S, Silvester W, Park D, Young J. Unexpectedly diverse Mesorhizobium strains and Rhizobium leguminosarum nodulate native legume genera of New Zealand, while introduced legume weeds are nodulated by Bradyrhizobium species. Appl Environ Microbiol. 2004;70:5980-7 pubmed publisher
    ..In addition, four isolates were identified as Rhizobium leguminosarum. In contrast, all sequences from isolates from introduced weeds aligned with Bradyrhizobium species but ..
  54. Richardson J, Hynes M, Oresnik I. A genetic locus necessary for rhamnose uptake and catabolism in Rhizobium leguminosarum bv. trifolii. J Bacteriol. 2004;186:8433-42 pubmed
    b>Rhizobium leguminosarum bv. trifolii mutants unable to catabolize the methyl-pentose rhamnose are unable to compete effectively for nodule occupancy...
  55. Edwards A, Frederix M, Wisniewski Dyé F, Jones J, Zorreguieta A, Downie J. The cin and rai quorum-sensing regulatory systems in Rhizobium leguminosarum are coordinated by ExpR and CinS, a small regulatory protein coexpressed with CinI. J Bacteriol. 2009;191:3059-67 pubmed publisher
    To understand how the Rhizobium leguminosarum raiI-raiR quorum-sensing system is regulated, we identified mutants with decreased levels of RaiI-made N-acyl homoserine lactones (AHLs)...
  56. Wielbo J, Marek Kozaczuk M, Mazur A, Kubik Komar A, Skorupska A. Genetic and metabolic divergence within a Rhizobium leguminosarum bv. trifolii population recovered from clover nodules. Appl Environ Microbiol. 2010;76:4593-600 pubmed publisher
    ..A population of Rhizobium leguminosarum bv...
  57. Ausmees N, Jonsson H, Hoglund S, Ljunggren H, Lindberg M. Structural and putative regulatory genes involved in cellulose synthesis in Rhizobium leguminosarum bv. trifolii. Microbiology. 1999;145 ( Pt 5):1253-62 pubmed
    Six genes involved in cellulose synthesis in Rhizobium leguminosarum bv. trifolii were identified using Tn5 mutagenesis...
  58. Janczarek M, Skorupska A. The Rhizobium leguminosarum bv. trifolii pssB gene product is an inositol monophosphatase that influences exopolysaccharide synthesis. Arch Microbiol. 2001;175:143-51 pubmed
    The pssB gene of Rhizobium leguminosarum bv. trifolii encodes a protein of 284 amino acids with sequence similarity to eukaryotic inositol monophosphatases. The gene was cloned and overexpressed in Escherichia coli...
  59. Fujishige N, Kapadia N, De Hoff P, Hirsch A. Investigations of Rhizobium biofilm formation. FEMS Microbiol Ecol. 2006;56:195-206 pubmed
    ..developed conditions for analyzing the ability of two fast-growing rhizobia, Sinorhizobium meliloti and Rhizobium leguminosarum bv...
  60. Janczarek M, Skorupska A. Rhizobium leguminosarum bv. trifolii rosR gene expression is regulated by catabolic repression. FEMS Microbiol Lett. 2009;291:112-9 pubmed publisher
    b>Rhizobium leguminosarum bv. trifolii rosR gene encodes a transcriptional regulator involved in the positive regulation of exopolysaccharide synthesis...
  61. Janczarek M, Jaroszuk Sciseł J, Skorupska A. Multiple copies of rosR and pssA genes enhance exopolysaccharide production, symbiotic competitiveness and clover nodulation in Rhizobium leguminosarum bv. trifolii. Antonie Van Leeuwenhoek. 2009;96:471-86 pubmed publisher
    b>Rhizobium leguminosarum bv. trifolii exopolysaccharide (EPS) plays an important role in determining symbiotic competence...
  62. Prell J, White J, Bourdes A, Bunnewell S, Bongaerts R, Poole P. Legumes regulate Rhizobium bacteroid development and persistence by the supply of branched-chain amino acids. Proc Natl Acad Sci U S A. 2009;106:12477-82 pubmed publisher
    ..However, metabolic exchange must be more complex, because effective N(2) fixation by Rhizobium leguminosarum bv viciae bacteroids requires either one of two broad-specificity amino acid ABC transporters (Aap and ..
  63. Brito B, Prieto R, Cabrera E, Mandrand Berthelot M, Imperial J, Ruiz Argüeso T, et al. Rhizobium leguminosarum hupE encodes a nickel transporter required for hydrogenase activity. J Bacteriol. 2010;192:925-35 pubmed publisher
    Synthesis of the hydrogen uptake (Hup) system in Rhizobium leguminosarum bv. viciae requires the function of an 18-gene cluster (hupSLCDEFGHIJK-hypABFCDEX)...
  64. Finnie C, Hartley N, Findlay K, Downie J. The Rhizobium leguminosarum prsDE genes are required for secretion of several proteins, some of which influence nodulation, symbiotic nitrogen fixation and exopolysaccharide modification. Mol Microbiol. 1997;25:135-46 pubmed
    NodO is a secreted protein from Rhizobium leguminosarum bv. viciae with a role in signalling during legume nodulation. A Tn5-induced mutant was identified that was defective in NodO secretion...
  65. Villaseñor T, Brom S, Dávalos A, Lozano L, Romero D, Los Santos A. Housekeeping genes essential for pantothenate biosynthesis are plasmid-encoded in Rhizobium etli and Rhizobium leguminosarum. BMC Microbiol. 2011;11:66 pubmed publisher
  66. Krol J, Skorupska A. Identification of genes in Rhizobium leguminosarum bv. trifolii whose products are homologues to a family of ATP-binding proteins. Microbiology. 1997;143 ( Pt 4):1389-94 pubmed
    ..and orf3, were identified adjacent to the exo133 mutation in a cosmid carrying the genomic DNA of Rhizobium leguminosarum bv. trifolii TA1...
  67. Bianciotto V, Andreotti S, Balestrini R, Bonfante P, Perotto S. Extracellular polysaccharides are involved in the attachment of Azospirillum brasilense and Rhizobium leguminosarum to arbuscular mycorrhizal structures. Eur J Histochem. 2001;45:39-49 pubmed
  68. Wexler M, Yeoman K, Stevens J, de Luca N, Sawers G, Johnston A. The Rhizobium leguminosarum tonB gene is required for the uptake of siderophore and haem as sources of iron. Mol Microbiol. 2001;41:801-16 pubmed
    In the N2-fixing bacterium Rhizobium leguminosarum, mutations in a homologue of tonB (tonB(Rl)) block the import of vicibactin and haem as iron sources in free-living bacteria...
  69. Krol J, Mazur A, Marczak M, Skorupska A. Syntenic arrangements of the surface polysaccharide biosynthesis genes in Rhizobium leguminosarum. Genomics. 2007;89:237-47 pubmed
    ..approach in the identification of genes required for the biosynthesis of different polysaccharides in Rhizobium leguminosarum bv. trifolii TA1 (RtTA1)...
  70. Miller L, Yost C, Hynes M, Alexandre G. The major chemotaxis gene cluster of Rhizobium leguminosarum bv. viciae is essential for competitive nodulation. Mol Microbiol. 2007;63:348-62 pubmed
    b>Rhizobium leguminosarum biovar viciae strain 3841 is a motile alpha-proteobacterium that can establish a nitrogen-fixing symbiosis within the roots of pea plants. In order to determine the contribution of chemotaxis to the lifestyle of R...
  71. Crossman L, Castillo Ramírez S, McAnnula C, Lozano L, Vernikos G, Acosta J, et al. A common genomic framework for a diverse assembly of plasmids in the symbiotic nitrogen fixing bacteria. PLoS ONE. 2008;3:e2567 pubmed publisher
    This work centres on the genomic comparisons of two closely-related nitrogen-fixing symbiotic bacteria, Rhizobium leguminosarum biovar viciae 3841 and Rhizobium etli CFN42...
  72. Tian C, Young J, Wang E, Tamimi S, Chen W. Population mixing of Rhizobium leguminosarum bv. viciae nodulating Vicia faba: the role of recombination and lateral gene transfer. FEMS Microbiol Ecol. 2010;73:563-76 pubmed publisher
    ..Eight distinct sublineages of Rhizobium leguminosarum bv. viciae (Rlv) were identified by concatenated sequences of chromosomal loci...
  73. Fry J, Wood M, Poole P. Investigation of myo-inositol catabolism in Rhizobium leguminosarum bv. viciae and its effect on nodulation competitiveness. Mol Plant Microbe Interact. 2001;14:1016-25 pubmed
    Three discrete loci required for growth on myo-inositol in Rhizobium leguminosarum bv. viciae have been characterized...
  74. Mazur A, Król J, Skorupska A. Isolation and sequencing of Rhizobium leguminosarum Bv. Trifolii PssN, PssO and PssP genes encoding the proteins involved in polymerization and translocation of exopolysaccharide. DNA Seq. 2001;12:1-12 pubmed
    b>Rhizobium leguminosarum bv. trifolii produces an acidic exopolysaccharide (EPS) that plays an important role in symbiotic interaction with clover plants. The sequence of 6...
  75. Hosie A, Allaway D, Galloway C, Dunsby H, Poole P. Rhizobium leguminosarum has a second general amino acid permease with unusually broad substrate specificity and high similarity to branched-chain amino acid transporters (Bra/LIV) of the ABC family. J Bacteriol. 2002;184:4071-80 pubmed
    Amino acid uptake by Rhizobium leguminosarum is dominated by two ABC transporters, the general amino acid permease (Aap) and the branched-chain amino acid permease (Bra(Rl))...
  76. Meloni S, Rey L, Sidler S, Imperial J, Ruiz Argüeso T, Palacios J. The twin-arginine translocation (Tat) system is essential for Rhizobium-legume symbiosis. Mol Microbiol. 2003;48:1195-207 pubmed
    ..Genetic determinants (tatABC genes) of the Tat system from Rhizobium leguminosarum bv. viciae were cloned and characterized, and a tatBC deletion mutant was constructed...
  77. Yost C, Del Bel K, Quandt J, Hynes M. Rhizobium leguminosarum methyl-accepting chemotaxis protein genes are down-regulated in the pea nodule. Arch Microbiol. 2004;182:505-13 pubmed
    Regulation of methyl-accepting chemotaxis protein (MCP) genes of Rhizobium leguminosarum was studied under symbiotic conditions...
  78. Young J, Crossman L, Johnston A, Thomson N, Ghazoui Z, Hull K, et al. The genome of Rhizobium leguminosarum has recognizable core and accessory components. Genome Biol. 2006;7:R34 pubmed
    b>Rhizobium leguminosarum is an alpha-proteobacterial N2-fixing symbiont of legumes that has been the subject of more than a thousand publications...
  79. Barr M, East A, Leonard M, Mauchline T, Poole P. In vivo expression technology (IVET) selection of genes of Rhizobium leguminosarum biovar viciae A34 expressed in the rhizosphere. FEMS Microbiol Lett. 2008;282:219-27 pubmed publisher
    ..used to identify genes that are specifically expressed in the rhizosphere of the pea-nodulating bacterium Rhizobium leguminosarum A34. A library of R...
  80. Chiurazzi M, Meza R, Lara M, Lahm A, Defez R, Iaccarino M, et al. The Rhizobium leguminosarum biovar phaseoli glnT gene, encoding glutamine synthetase III. Gene. 1992;119:1-8 pubmed
    Plasmid pGE203 contains the Rhizobium leguminosarum biovar phaseoli glnT locus. Glutamine synthetase III (GSIII) was purified from a glutamine auxotrophic strain of Klebsiella pneumoniae carrying this plasmid. Sequencing of a 2...
  81. Hosie A, Allaway D, Jones M, Walshaw D, Johnston A, Poole P. Solute-binding protein-dependent ABC transporters are responsible for solute efflux in addition to solute uptake. Mol Microbiol. 2001;40:1449-59 pubmed
    ..e. the general amino acid permease (Aap) and the branched-chain amino acid permease (Bra) of Rhizobium leguminosarum and the histidine permease (His) of Salmonella typhimurium, are bidirectional, being responsible for ..
  82. Wisniewski Dyé F, Jones J, Chhabra S, Downie J. raiIR genes are part of a quorum-sensing network controlled by cinI and cinR in Rhizobium leguminosarum. J Bacteriol. 2002;184:1597-606 pubmed
    Analysis of N-acyl-L-homoserine lactones (AHLs) produced by Rhizobium leguminosarum bv. viciae indicated that there may be a network of quorum-sensing regulatory systems producing multiple AHLs in this species...
  83. Yost C, Clark K, Del Bel K, Hynes M. Characterization of the nodulation plasmid encoded chemoreceptor gene mcpG from Rhizobium leguminosarum. BMC Microbiol. 2003;3:1 pubmed
    ..Methyl accepting chemotaxis proteins are sensory proteins important in chemotaxis of numerous bacteria, but their involvement in Rhizobium chemotaxis is unclear and merits further investigation...
  84. Quandt J, Clark R, Venter A, Clark S, Twelker S, Hynes M. Modified RP4 and Tn5-Mob derivatives for facilitated manipulation of large plasmids in Gram-negative bacteria. Plasmid. 2004;52:1-12 pubmed
    ..As an example of the use of these tools, three Rhizobium leguminosarum bv...
  85. Yajima A, Van Brussel A, Schripsema J, Nukada T, Yabuta G. Synthesis and stereochemistry-activity relationship of small bacteriocin, an autoinducer of the symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum. Org Lett. 2008;10:2047-50 pubmed publisher
    The four stereoisomers of small bacteriocin, an autoinducer of the symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum, were synthesized via a versatile methodology for 3'-hydroxyacyl homoserine lactones based on the Nagao ..
  86. Prell J, Mulley G, Haufe F, White J, Williams A, Karunakaran R, et al. The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum. Mol Microbiol. 2012;84:117-29 pubmed publisher
    Mutation of ptsP encoding EI(Ntr) of the PTS(Ntr) system in Rhizobium leguminosarum strain Rlv3841 caused a pleiotropic phenotype as observed with many bacteria...
  87. Schluter A, Nohlen M, Kramer M, Defez R, Priefer U. The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation. Microbiology. 2000;146 ( Pt 11):2987-96 pubmed
    A Rhizobium leguminosarum bv. viciae VF39 gene (glnD) encoding the uridylyltransferase/uridylyl-removing enzyme, which constitutes the sensory component of the nitrogen regulation (ntr) system, was identified, cloned and characterized...
  88. Ma W, Guinel F, Glick B. Rhizobium leguminosarum biovar viciae 1-aminocyclopropane-1-carboxylate deaminase promotes nodulation of pea plants. Appl Environ Microbiol. 2003;69:4396-402 pubmed
    ..ACC) deaminase gene was isolated and characterized from one of the ACC deaminase-producing rhizobia, Rhizobium leguminosarum bv. viciae 128C53K. ACC deaminase degrades ACC, the immediate precursor of ethylene in higher plants...
  89. Díaz Mireles E, Wexler M, Sawers G, Bellini D, Todd J, Johnston A. The Fur-like protein Mur of Rhizobium leguminosarum is a Mn(2+)-responsive transcriptional regulator. Microbiology. 2004;150:1447-56 pubmed
    In wild-type Rhizobium leguminosarum, the sitABCD operon specifies a Mn(2+) transporter whose expression is severely reduced in cells grown in the presence of this metal. Mutations in the R...
  90. Todd J, Sawers G, Johnston A. Proteomic analysis reveals the wide-ranging effects of the novel, iron-responsive regulator RirA in Rhizobium leguminosarum bv. viciae. Mol Genet Genomics. 2005;273:197-206 pubmed
    The wide-ranging effects of RirA, a novel Fe-responsive regulator of gene expression in Rhizobium leguminosarum bv. viciae, were monitored on 2D gels...
  91. McIntyre H, Davies H, Hore T, Miller S, Dufour J, Ronson C. Trehalose biosynthesis in Rhizobium leguminosarum bv. trifolii and its role in desiccation tolerance. Appl Environ Microbiol. 2007;73:3984-92 pubmed
    b>Rhizobium leguminosarum bv. trifolii forms nitrogen-fixing root nodules on the pasture legume Trifolium repens, and T. repens seed is often coated with a compatible R. leguminosarum bv. trifolii strain prior to sowing...
  92. Gilbert K, Vanderlinde E, Yost C. Mutagenesis of the carboxy terminal protease CtpA decreases desiccation tolerance in Rhizobium leguminosarum. FEMS Microbiol Lett. 2007;272:65-74 pubmed
    To better understand the role of proteases in Rhizobium leguminosarum biovar viciae, a gene with homology to the carboxy-terminal protease (CtpA), which belongs to a novel group of serine proteases, was studied...