agrobacterium tumefaciens

Summary

Summary: A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.

Top Publications

  1. Picard C, Ponsonnet C, Paget E, Nesme X, Simonet P. Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction. Appl Environ Microbiol. 1992;58:2717-22 pubmed
    ..Two microorganisms were used as models: Agrobacterium tumefaciens, which is naturally absent from the soil used and was inoculated to calibrate the validity of the ..
  2. Kirchner T, Niehaus M, Debener T, Schenk M, Herde M. Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata. PLoS ONE. 2017;12:e0185429 pubmed publisher
    ..This study paves the way for experimental strategies involving the phenotypic assessment of gene lesions by CRISPR which do not require germline transmission. ..
  3. Qi X, Su X, Guo H, Qi J, Cheng H. A ku70 null mutant improves gene targeting frequency in the fungal pathogen Verticillium dahliae. World J Microbiol Biotechnol. 2015;31:1889-97 pubmed
    ..A V. dahliae 991 ΔVdku70 null mutant strain was generated using Agrobacterium tumefaciens-mediated transformation...
  4. Leuzinger K, Dent M, Hurtado J, Stahnke J, Lai H, Zhou X, et al. Efficient agroinfiltration of plants for high-level transient expression of recombinant proteins. J Vis Exp. 2013;: pubmed publisher
    ..It will greatly facilitate the development of pharmaceutical proteins and promote science education. ..
  5. Goswami S, Das S, Datta S. Understanding the role of residues around the active site tunnel towards generating a glucose-tolerant ?-glucosidase from Agrobacterium tumefaciens 5A. Protein Eng Des Sel. 2017;30:523-530 pubmed publisher
    ..six mutations of a moderately glucose-tolerant ?-glucosidase (H0HC94) from the mesophilic bacterium Agrobacterium tumefaciens 5A...
  6. Salyaev R, Rekoslavskaya N, Stolbikov A, Tretyakova A. The study of immunogenicity of the antigenic protein of high risk oncogenic type of the human papillomavirus HPV16 L1 produced in the plant expression system on the base of transgenic tomato. Dokl Biochem Biophys. 2017;474:186-188 pubmed publisher
    ..This level of antibodies was retained until the end of the study. ..
  7. Ehrle H, Guidry J, Iacovetto R, Salisbury A, Sandidge D, Bowman G. Polar Organizing Protein PopZ Is Required for Chromosome Segregation in Agrobacterium tumefaciens. J Bacteriol. 2017;199: pubmed publisher
    ..Here, we characterize the localization and function of PopZ in Agrobacterium tumefaciens, a genetically related species with distinct anatomy...
  8. Ding L, Chen Y, Wei X, Ni M, Zhang J, Wang H, et al. Laboratory evaluation of transgenic Populus davidiana×Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm. PLoS ONE. 2017;12:e0178754 pubmed publisher
    ..cunea larvae are more sensitive to the insecticidal proteins compared to L. dispar. Transgenic poplar lines toxic to L. dispar and H. cunea could be used to provide Lepidoptera pest resistance to selected strains of poplar trees. ..
  9. Martínez Moñino A, Zapata Perez R, García Saura A, Gil Ortiz F, Pérez Gilabert M, Sanchez Ferrer A. Characterization and mutational analysis of a nicotinamide mononucleotide deamidase from Agrobacterium tumefaciens showing high thermal stability and catalytic efficiency. PLoS ONE. 2017;12:e0174759 pubmed publisher
    ..In this study, we present the first characterization of an NMN deamidase from an alphaproteobacterium, Agrobacterium tumefaciens (AtCinA). The enzyme was active over a broad pH range, with an optimum at pH 7.5...

More Information

Publications25

  1. Mora Lugo R, Zimmermann J, Rizk A, Fernandez Lahore M. Development of a transformation system for Aspergillus sojae based on the Agrobacterium tumefaciens-mediated approach. BMC Microbiol. 2014;14:247 pubmed publisher
    ..In this study, an Agrobacterium tumefaciens-mediated transformation (ATMT) system for A. sojae was developed and its applicability evaluated...
  2. Gawehns F, Houterman P, Ichou F, Michielse C, Hijdra M, Cornelissen B, et al. The Fusarium oxysporum effector Six6 contributes to virulence and suppresses I-2-mediated cell death. Mol Plant Microbe Interact. 2014;27:336-48 pubmed publisher
    ..Heterologous expression of SIX6 did not affect growth of Agrobacterium tumefaciens in Nicotiana benthamiana leaves or susceptibility of Arabidopsis thaliana toward Verticillium dahliae, ..
  3. Gao J, Liu T, Chen J. Insertional mutagenesis and cloning of the gene required for the biosynthesis of the non-host-specific toxin in Cochliobolus lunatus that causes maize leaf spot. Phytopathology. 2014;104:332-9 pubmed publisher
    ..In the current work, Agrobacterium tumefaciens-mediated transformation (ATMT) was employed to tag the gene required for the biosynthesis of the toxin...
  4. Gordon J, Christie P. The Agrobacterium Ti Plasmids. Microbiol Spectr. 2014;2: pubmed publisher
    b>Agrobacterium tumefaciens is a plant pathogen with the capacity to deliver a segment of oncogenic DNA carried on a large plasmid called the tumor-inducing or Ti plasmid to susceptible plant cells. A...
  5. Nguyen K, Ho Q, Do L, Mai L, Pham D, Tran H, et al. A new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus Aspergillus oryzae using Agrobacterium tumefaciens-mediated transformation. World J Microbiol Biotechnol. 2017;33:107 pubmed publisher
    ..In this study, we show that Agrobacterium tumefaciens-mediated transformation (ATMT) method is very efficient for deletion of the pyrG gene in different ..
  6. Liu W, Wang Y, Zhang Z, Wang M, Lv Q, Liu H, et al. Generation and characterization of caprine chymosin in corn seed. Protein Expr Purif. 2017;135:78-82 pubmed publisher
    ..5 U/mg. These results indicate that we have successfully established a technology for generating corn seed-derived caprine chymosin for potential use in the dairy industry. ..
  7. Kadam U, Chavhan R, Schulz B, Irudayaraj J. Single molecule Raman spectroscopic assay to detect transgene from GM plants. Anal Biochem. 2017;532:60-63 pubmed publisher
    ..The assay allows us to detect and quantify the transgenes as low as 0.10 pg without need for PCR-amplification. This technology is relatively cheap, quick, simple, and suitable for detection at low target concentration. ..
  8. Tuo D, Fu L, Shen W, Li X, Zhou P, Yan P. Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation. Virology. 2017;510:99-103 pubmed publisher
    A novel Rhizobium radiobacter (synonym Agrobacterium tumefaciens)-mediated approach was developed to generate stable infectious clones of plant viruses. This method uses R...
  9. Shilo S, Tripathi P, Melamed Bessudo C, Tzfadia O, Muth T, Levy A. T-DNA-genome junctions form early after infection and are influenced by the chromatin state of the host genome. PLoS Genet. 2017;13:e1006875 pubmed publisher
    b>Agrobacterium tumefaciens mediated T-DNA integration is a common tool for plant genome manipulation. However, there is controversy regarding whether T-DNA integration is biased towards genes or randomly distributed throughout the genome...
  10. Redzej A, Ukleja M, Connery S, Trokter M, Felisberto Rodrigues C, Cryar A, et al. Structure of a VirD4 coupling protein bound to a VirB type IV secretion machinery. EMBO J. 2017;36:3080-3095 pubmed publisher
    ..Given the central role of VirD4 in type IV secretion, our study provides mechanistic insights on a process that mediates the dangerous spread of antibiotic resistance genes among bacterial populations. ..
  11. Bernardinelli G, Högberg B. Entirely enzymatic nanofabrication of DNA-protein conjugates. Nucleic Acids Res. 2017;45:e160 pubmed publisher
    ..of DNA-protein conjugates using a new self-labeling tag derived from a truncated VirD2 protein of Agrobacterium tumefaciens. Using our previously reported MOSIC method for enzymatic ssDNA oligo production, we outline a pipeline ..
  12. Howell M, Aliashkevich A, Salisbury A, Cava F, Bowman G, Brown P. Absence of the Polar Organizing Protein PopZ Results in Reduced and Asymmetric Cell Division in Agrobacterium tumefaciens. J Bacteriol. 2017;199: pubmed publisher
    i>Agrobacterium tumefaciens is a rod-shaped bacterium that grows by polar insertion of new peptidoglycan during cell elongation...
  13. Koh C, Liu Y, Moehninsi -, Du M, Ji L. Molecular characterization of KU70 and KU80 homologues and exploitation of a KU70-deficient mutant for improving gene deletion frequency in Rhodosporidium toruloides. BMC Microbiol. 2014;14:50 pubmed publisher
    ..toruloides ATCC 10657 via Agrobacterium tumefaciens-mediated transformation. To further improve targeting frequency, the KU70 and KU80 homologs in R...
  14. Soares da Costa T, Patel M, Desbois S, Gupta R, Faou P, Perugini M. Identification of a dimeric KDG aldolase from Agrobacterium tumefaciens. Proteins. 2017;85:2058-2065 pubmed publisher
    b>Agrobacterium tumefaciens is a Gram-negative bacterium and causative agent of Crown Gall disease that infects a variety of economically important plants. The annotated A...
  15. van Frankenhuyzen J, Trevors J, Flemming C, Lee H, Habash M. Optimization, validation, and application of a real-time PCR protocol for quantification of viable bacterial cells in municipal sewage sludge and biosolids using reporter genes and Escherichia coli. J Ind Microbiol Biotechnol. 2013;40:1251-61 pubmed
    ..Reporter genes from genetically marked Pseudomonas sp. UG14Lr and Agrobacterium tumefaciens 542 cells were spiked into samples of primary sludge, and anaerobically digested and Lystek-treated ..
  16. Fadel M, El Gebaly R, Mohamed S, Abdelbacki A. Biophysical control of the growth of Agrobacterium tumefaciens using extremely low frequency electromagnetic waves at resonance frequency. Biochem Biophys Res Commun. 2017;494:365-371 pubmed publisher
    Isolated Agrobacterium tumefaciens was exposed to different extremely low frequencies of square amplitude modulated waves (QAMW) from two generators to determine the resonance frequency that causes growth inhibition...