azotobacter vinelandii

Summary

Summary: A species of gram-negative, aerobic bacteria first isolated from soil in Vineland, New Jersey. Ammonium and nitrate are used as nitrogen sources by this bacterium. It is distinguished from other members of its genus by the ability to use rhamnose as a carbon source. (From Bergey's Manual of Determinative Bacteriology, 9th ed)

Top Publications

  1. Cartini F, Remelli W, Dos Santos P, Papenbrock J, Pagani S, Forlani F. Mobilization of sulfane sulfur from cysteine desulfurases to the Azotobacter vinelandii sulfurtransferase RhdA. Amino Acids. 2011;41:141-50 pubmed publisher
    Mobilization of the L-cysteine sulfur for the persulfuration of the rhodanese of Azotobacter vinelandii, RhdA, can be mediated by the A. vinelandii cysteine desulfurases, IscS and NifS...
  2. Cereda A, Carpen A, Picariello G, Tedeschi G, Pagani S. The lack of rhodanese RhdA affects the sensitivity of Azotobacter vinelandii to oxidative events. Biochem J. 2009;418:135-43 pubmed publisher
    The rhdA gene of Azotobacter vinelandii codes for RhdA, a rhodanese-domain protein with an active-site loop structure which has not currently been found in proteins of the rhodanese-homology superfamily...
  3. Corbett M, Hu Y, Fay A, Ribbe M, Hedman B, Hodgson K. Structural insights into a protein-bound iron-molybdenum cofactor precursor. Proc Natl Acad Sci U S A. 2006;103:1238-43 pubmed
    ..Through the comparison of Azotobacter vinelandii strains expressing the NifEN protein in the presence or absence of the nifB gene, the structure of a NifEN-..
  4. Nabuurs S, Westphal A, van Mierlo C. Noncooperative Formation of the off-pathway molten globule during folding of the alpha-beta parallel protein apoflavodoxin. J Am Chem Soc. 2009;131:2739-46 pubmed publisher
    ..The molten globule that is observed during folding of flavodoxin from Azotobacter vinelandii is a kinetically off-pathway species, as it has to unfold before the native state of the protein can be ..
  5. Zheng L, Cash V, Flint D, Dean D. Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii. J Biol Chem. 1998;273:13264-72 pubmed
    ..desulfurization activity previously described for the NifS protein was purified from a strain of Azotobacter vinelandii deleted for the nifS gene...
  6. Trujillo Roldán M, Moreno S, Espin G, Galindo E. The roles of oxygen and alginate-lyase in determining the molecular weight of alginate produced by Azotobacter vinelandii. Appl Microbiol Biotechnol. 2004;63:742-7 pubmed
    An Azotobacter vinelandii mutant lacking alginate-lyase (SML2) and the wild type (ATCC 9046) were used to discriminate between the roles of the polymerase complex and alginate-lyase in the synthesis of alginate in cultures conducted under ..
  7. Visser N, Westphal A, van Hoek A, van Mierlo C, Visser A, van Amerongen H. Tryptophan-tryptophan energy migration as a tool to follow apoflavodoxin folding. Biophys J. 2008;95:2462-9 pubmed publisher
    Submolecular details of Azotobacter vinelandii apoflavodoxin (apoFD) (un)folding are revealed by time-resolved fluorescence anisotropy using wild-type protein and variants lacking one or two of apoFD's three tryptophans...
  8. Reyes Ramírez F, Little R, Dixon R. Role of Escherichia coli nitrogen regulatory genes in the nitrogen response of the Azotobacter vinelandii NifL-NifA complex. J Bacteriol. 2001;183:3076-82 pubmed
    ..NifL inhibits the activity of the nitrogen fixation (nif)-specific transcriptional activator NifA in Azotobacter vinelandii in response to molecular oxygen and fixed nitrogen. Although the mechanism whereby the A...
  9. Engel R, Westphal A, Huberts D, Nabuurs S, Lindhoud S, Visser A, et al. Macromolecular crowding compacts unfolded apoflavodoxin and causes severe aggregation of the off-pathway intermediate during apoflavodoxin folding. J Biol Chem. 2008;283:27383-94 pubmed publisher
    ..Apparently, in the cell, apoflavodoxin requires the help of chaperones like Trigger Factor and the DnaK system for efficient folding. ..

More Information

Publications89

  1. Núñez C, Bogachev A, Guzman G, Tello I, Guzmán J, Espín G. The Na+-translocating NADH : ubiquinone oxidoreductase of Azotobacter vinelandii negatively regulates alginate synthesis. Microbiology. 2009;155:249-56 pubmed publisher
    b>Azotobacter vinelandii is a nitrogen-fixing soil bacterium that produces the exopolysaccharide alginate. In this report we describe the isolation and characterization of A...
  2. Hefti M, Van Vugt Van der Toorn C, Dixon R, Vervoort J. A novel purification method for histidine-tagged proteins containing a thrombin cleavage site. Anal Biochem. 2001;295:180-5 pubmed
    ..Our method is compared with a more conventional method for the purification of the Azotobacter vinelandii NIFL PAS domain, overexpressed in Escherichia coli...
  3. Slavny P, Little R, Salinas P, Clarke T, Dixon R. Quaternary structure changes in a second Per-Arnt-Sim domain mediate intramolecular redox signal relay in the NifL regulatory protein. Mol Microbiol. 2010;75:61-75 pubmed publisher
    ..Multiple PAS domains are often found within a single protein. The NifL regulatory protein from Azotobacter vinelandii contains tandem PAS domains, the most N-terminal of which, PAS1, contains a FAD cofactor and is ..
  4. Little R, Salinas P, Slavny P, Clarke T, Dixon R. Substitutions in the redox-sensing PAS domain of the NifL regulatory protein define an inter-subunit pathway for redox signal transmission. Mol Microbiol. 2011;82:222-35 pubmed publisher
    ..The NifL regulatory protein from Azotobacter vinelandii senses the oxygen status of the cell via an FAD cofactor accommodated within the first of two amino-..
  5. Soboh B, Igarashi R, Hernandez J, Rubio L. Purification of a NifEN protein complex that contains bound molybdenum and a FeMo-Co precursor from an Azotobacter vinelandii DeltanifHDK strain. J Biol Chem. 2006;281:36701-9 pubmed
    ..We have improved the purification of the his-NifEN protein from a DeltanifHDK strain of Azotobacter vinelandii and have found that the amounts of iron and molybdenum within NifEN were significantly higher than those ..
  6. Díaz Barrera A, Pena C, Galindo E. The oxygen transfer rate influences the molecular mass of the alginate produced by Azotobacter vinelandii. Appl Microbiol Biotechnol. 2007;76:903-10 pubmed
    ..0 mmol l(-1) h(-1) was 7.0 times higher (1,560 kDa) than at 9.0 mmol l(-1) h(-1) (220 kDa). An increase in molecular mass can be a bacterial response to adverse nutritional conditions such as oxygen limitation. ..
  7. Segura D, Vite O, Romero Y, Moreno S, Castañeda M, Espín G. Isolation and characterization of Azotobacter vinelandii mutants impaired in alkylresorcinol synthesis: alkylresorcinols are not essential for cyst desiccation resistance. J Bacteriol. 2009;191:3142-8 pubmed publisher
    During encystment of Azotobacter vinelandii, a family of alkylresorcinols (ARs) and alkylpyrones (APs) are synthesized...
  8. Rediers H, Vanderleyden J, De Mot R. Azotobacter vinelandii: a Pseudomonas in disguise?. Microbiology. 2004;150:1117-9 pubmed
  9. Hartmann M, Duun A, Markussen S, Grasdalen H, Valla S, Skjak Braek G. Time-resolved 1H and 13C NMR spectroscopy for detailed analyses of the Azotobacter vinelandii mannuronan C-5 epimerase reaction. Biochim Biophys Acta. 2002;1570:104-12 pubmed
    AlgE2, AlgE4, and AlgE6 are members of a family of mannuronan C-5 epimerases encoded by Azotobacter vinelandii, and are active in the biosynthesis of alginate, where they catalyze the post-polymerization conversion of beta-D-mannuronic ..
  10. Yoshizawa J, Blank M, Fay A, Lee C, Wiig J, Hu Y, et al. Optimization of FeMoco maturation on NifEN. J Am Chem Soc. 2009;131:9321-5 pubmed publisher
  11. Maier R, Moshiri F. Role of the Azotobacter vinelandii nitrogenase-protective shethna protein in preventing oxygen-mediated cell death. J Bacteriol. 2000;182:3854-7 pubmed
    b>Azotobacter vinelandii strains lacking the nitrogenase-protective Shethna protein lost viability upon carbon-substrate deprivation in the presence of oxygen...
  12. Bollen Y, van Mierlo C. Protein topology affects the appearance of intermediates during the folding of proteins with a flavodoxin-like fold. Biophys Chem. 2005;114:181-9 pubmed
    ..as well? This question is addressed by comparing the folding data recently obtained on apoflavodoxin from Azotobacter vinelandii with those available on all three other alpha-beta parallel proteins the kinetic folding mechanism of ..
  13. Trujillo Roldán M, Pena C, Ramirez O, Galindo E. Effect of oscillating dissolved oxygen tension on the production of alginate by Azotobacter vinelandii. Biotechnol Prog. 2001;17:1042-8 pubmed
    ..oscillating dissolved oxygen tension (DOT) on the metabolism of an exopolysaccharide-producing bacteria (Azotobacter vinelandii) was investigated, particularly on the mean molecular weight (MMW) of the alginate produced...
  14. Ribbe M, Hu Y, Guo M, Schmid B, Burgess B. The FeMoco-deficient MoFe protein produced by a nifH deletion strain of Azotobacter vinelandii shows unusual P-cluster features. J Biol Chem. 2002;277:23469-76 pubmed
    The His-tag MoFe protein expressed by the nifH deletion strain Azotobacter vinelandii DJ1165 (Delta(nifH) MoFe protein) was purified in large quantity...
  15. Díaz Barrera A, Silva P, Berrios J, Acevedo F. Manipulating the molecular weight of alginate produced by Azotobacter vinelandii in continuous cultures. Bioresour Technol. 2010;101:9405-8 pubmed publisher
    Alginate production by Azotobacter vinelandii in chemostat cultures was evaluated at different dilution rates (D) and inlet sucrose concentrations of 5 and 20 g l(-1)...
  16. Schindelin H, Kisker C, Schlessman J, Howard J, Rees D. Structure of ADP x AIF4(-)-stabilized nitrogenase complex and its implications for signal transduction. Nature. 1997;387:370-6 pubmed
    ..Interactions in the nitrogenase complex have broad implications for signal and energy transduction mechanisms in multiprotein complexes. ..
  17. Chen K, Jung Y, Bonagura C, Tilley G, Prasad G, Sridhar V, et al. Azotobacter vinelandii ferredoxin I: a sequence and structure comparison approach to alteration of [4Fe-4S]2+/+ reduction potential. J Biol Chem. 2002;277:5603-10 pubmed
    The reduction potential (E(0)') of the [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I (AvFdI) and related ferredoxins is approximately 200 mV more negative than the corresponding clusters of Peptostreptococcus ..
  18. Einsle O, Tezcan F, Andrade S, Schmid B, Yoshida M, Howard J, et al. Nitrogenase MoFe-protein at 1.16 A resolution: a central ligand in the FeMo-cofactor. Science. 2002;297:1696-700 pubmed
    ..The presence of a nitrogen atom in the cofactor would have important implications for the mechanism of dinitrogen reduction by nitrogenase. ..
  19. Schmid B, Ribbe M, Einsle O, Yoshida M, Thomas L, Dean D, et al. Structure of a cofactor-deficient nitrogenase MoFe protein. Science. 2002;296:352-6 pubmed
    ..A predominantly positively charged funnel is revealed; this funnel is of sufficient size to accommodate insertion of the negatively charged cofactor...
  20. Sashidhar B, Podile A. Transgenic expression of glucose dehydrogenase in Azotobacter vinelandii enhances mineral phosphate solubilization and growth of sorghum seedlings. Microb Biotechnol. 2009;2:521-9 pubmed publisher
    ..Escherichia coli gcd, cloned under the control of glnA and pts promoters, was mobilized into Azotobacter vinelandii AvOP and expressed...
  21. Wichard T, Bellenger J, Loison A, Kraepiel A. Catechol siderophores control tungsten uptake and toxicity in the nitrogen-fixing bacterium Azotobacter vinelandii. Environ Sci Technol. 2008;42:2408-13 pubmed
    ..in the molybdenum-nitrogenase enzyme and is thus an important micronutrient for N2-fixing bacteria such as Azotobacter vinelandii (A. vinelandii)...
  22. Hernández Eligio A, Moreno S, Castellanos M, Castañeda M, Núñez C, Muriel Millán L, et al. RsmA post-transcriptionally controls PhbR expression and polyhydroxybutyrate biosynthesis in Azotobacter vinelandii. Microbiology. 2012;158:1953-63 pubmed publisher
    In Azotobacter vinelandii the two-component GacS/GacA system is required for synthesis of polyhydroxybutyrate (PHB) and of the exopolysaccharide alginate...
  23. Han J, Newton W. Differentiation of acetylene-reduction sites by stereoselective proton addition during Azotobacter vinelandii nitrogenase-catalyzed C2D2 reduction. Biochemistry. 2004;43:2947-56 pubmed
    The interactions of acetylene with its binding site(s) on the FeMo cofactor of the MoFe protein of Azotobacter vinelandii nitrogenase were probed using C(2)D(2)...
  24. Gao Sheridan H, Pershad H, Armstrong F, Burgess B. Discovery of a novel ferredoxin from Azotobacter vinelandii containing two [4Fe-4S] clusters with widely differing and very negative reduction potentials. J Biol Chem. 1998;273:5514-9 pubmed
    ..Here we report the purification of a novel ferredoxin (FdIII) from Azotobacter vinelandii which brings to 12 the number of small [Fe-S] proteins that have now been reported from this organism...
  25. Townsend K, Busse K, Kressler J, Scholz C. Contact angle, WAXS, and SAXS analysis of poly(beta-hydroxybutyrate) and poly(ethylene glycol) block copolymers obtained via Azotobacter vinelandii UWD. Biotechnol Prog. 2005;21:959-64 pubmed
    ..and cell interactions of copolymers obtained by a poly(ethylene glycol) (PEG)-modulated fermentation of Azotobacter vinelandii UWD...
  26. Gaytán I, Pena C, Núñez C, Córdova M, Espín G, Galindo E. Azotobacter vinelandii lacking the Na(+)-NQR activity: a potential source for producing alginates with improved properties and at high yield. World J Microbiol Biotechnol. 2012;28:2731-40 pubmed publisher
    The mutant ATCN4 strain of Azotobacter vinelandii, which lacks the Na(+)-NQR activity and results in an alginate overproduction (highly mucoid phenotype), was cultured in shake flasks in minimal and rich medium, and the chemical ..
  27. Steigedal M, Sletta H, Moreno S, Maerk M, Christensen B, Bjerkan T, et al. The Azotobacter vinelandii AlgE mannuronan C-5-epimerase family is essential for the in vivo control of alginate monomer composition and for functional cyst formation. Environ Microbiol. 2008;10:1760-70 pubmed publisher
    ..In the genome of the alginate-producing bacterium Azotobacter vinelandii genes encoding one periplasmic (AlgG) and seven secreted such epimerases (AlgE1-7) have been identified...
  28. Hernández Eligio A, Castellanos M, Moreno S, Espín G. Transcriptional activation of the Azotobacter vinelandii polyhydroxybutyrate biosynthetic genes phbBAC by PhbR and RpoS. Microbiology. 2011;157:3014-23 pubmed publisher
    We previously showed that in Azotobacter vinelandii, accumulation of polyhydroxybutyrate (PHB) occurs mainly during the stationary phase, and that a mutation in phbR, encoding a transcriptional regulator of the AraC family, reduces PHB ..
  29. Remelli W, Cereda A, Papenbrock J, Forlani F, Pagani S. The rhodanese RhdA helps Azotobacter vinelandii in maintaining cellular redox balance. Biol Chem. 2010;391:777-84 pubmed publisher
    The tandem domain rhodanese-homology protein RhdA of Azotobacter vinelandii shows an active-site loop structure that confers structural peculiarity in the environment of its catalytic cysteine residue...
  30. Bollen Y, Nabuurs S, van Berkel W, van Mierlo C. Last in, first out: the role of cofactor binding in flavodoxin folding. J Biol Chem. 2005;280:7836-44 pubmed
    ..the influence of the flavin mononucleotide (FMN) cofactor on the global stability and folding kinetics of Azotobacter vinelandii holoflavodoxin. Earlier studies have revealed that A...
  31. Segura D, Guzman J, Espin G. Azotobacter vinelandii mutants that overproduce poly-beta-hydroxybutyrate or alginate. Appl Microbiol Biotechnol. 2003;63:159-63 pubmed
    b>Azotobacter vinelandii produces two polymers of industrial importance, i.e. alginate and poly-beta-hydroxybutyrate (PHB)...
  32. Fenske D, Gnida M, Schneider K, Meyer Klaucke W, Schemberg J, Henschel V, et al. A new type of metalloprotein: The Mo storage protein from azotobacter vinelandii contains a polynuclear molybdenum-oxide cluster. Chembiochem. 2005;6:405-13 pubmed
    b>Azotobacter vinelandii is a diazotrophic bacterium characterized by the outstanding capability of storing Mo in a special storage protein, which guarantees Mo-dependent nitrogen fixation even under growth conditions of extreme Mo ..
  33. Mejia M, Segura D, Espin G, Galindo E, Pena C. Two-stage fermentation process for alginate production by Azotobacter vinelandii mutant altered in poly-beta-hydroxybutyrate (PHB) synthesis. J Appl Microbiol. 2010;108:55-61 pubmed publisher
    ..conducted first under non-oxygen-limited conditions, and later under oxygen-limited conditions, was used to improve alginate production by Azotobacter vinelandii (AT6), a strain impaired in poly-beta-hydroxybutyrate (PHB) production.
  34. Schmid B, Einsle O, Chiu H, Willing A, Yoshida M, Howard J, et al. Biochemical and structural characterization of the cross-linked complex of nitrogenase: comparison to the ADP-AlF4(-)-stabilized structure. Biochemistry. 2002;41:15557-65 pubmed
  35. Forlani F, Cereda A, Freuer A, Nimtz M, Leimkuhler S, Pagani S. The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form. FEBS Lett. 2005;579:6786-90 pubmed
    After heterologous expression in Escherichia coli, the Azotobacter vinelandii rhodanese RhdA is purified in a persulfurated form (RhdA-SSH). We identified l-cysteine as the most effective sulfur source in producing RhdA-SSH. An E...
  36. Swartz L, Kuchinskas M, Li H, Poulos T, Lanzilotta W. Redox-dependent structural changes in the Azotobacter vinelandii bacterioferritin: new insights into the ferroxidase and iron transport mechanism. Biochemistry. 2006;45:4421-8 pubmed
    ..0) forms of the native Azotobacter vinelandii bacterioferritin to 2.7 and 2.0 A resolution, respectively...
  37. Bellenger J, Wichard T, Kraepiel A. Vanadium requirements and uptake kinetics in the dinitrogen-fixing bacterium Azotobacter vinelandii. Appl Environ Microbiol. 2008;74:1478-84 pubmed publisher
    ..compounds across a range of concentrations of vanadium in diazotrophic cultures of the soil bacterium Azotobacter vinelandii. In strain CA11...
  38. Ertesvag H, Høidal H, Skjak Braek G, Valla S. The Azotobacter vinelandii mannuronan C-5-epimerase AlgE1 consists of two separate catalytic domains. J Biol Chem. 1998;273:30927-32 pubmed
    The Azotobacter vinelandii enzyme AlgE1 is a member of a family of secreted mannuronan C-5-epimerases...
  39. Hamilton T, Ludwig M, Dixon R, Boyd E, Dos Santos P, Setubal J, et al. Transcriptional profiling of nitrogen fixation in Azotobacter vinelandii. J Bacteriol. 2011;193:4477-86 pubmed publisher
    ..b>Azotobacter vinelandii, an obligate aerobe, fixes nitrogen via the oxygen-sensitive Mo nitrogenase but is also able to fix ..
  40. Hu Y, Fay A, Lee C, Ribbe M. P-cluster maturation on nitrogenase MoFe protein. Proc Natl Acad Sci U S A. 2007;104:10424-9 pubmed
    ..Furthermore, we provide spectroscopic evidence that the [4Fe-4S] cluster-like fragments can be converted to P-clusters, thereby firmly establishing the physiological relevance of the previously identified P-cluster precursor...
  41. Nabuurs S, van Mierlo C. Interrupted hydrogen/deuterium exchange reveals the stable core of the remarkably helical molten globule of alpha-beta parallel protein flavodoxin. J Biol Chem. 2010;285:4165-72 pubmed publisher
    ..The molten globule that is observed during folding of alpha-beta parallel flavodoxin from Azotobacter vinelandii is a remarkably non-native species...
  42. Blatny J, Ertesvåg H, Nes I, Valla S. Heterologous gene expression in Lactococcus lactis; expression of the Azotobacter vinelandii algE6 gene product displaying mannuronan C-5 epimerase activity. FEMS Microbiol Lett. 2003;227:229-35 pubmed
    The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1-7 can be used to improve the properties of the commercially important polysaccharide alginate that is widely used in a variety of products, such as food and pharmaceuticals...
  43. Svanem B, Strand W, Ertesvag H, Skjak Braek G, Hartmann M, Barbeyron T, et al. The catalytic activities of the bifunctional Azotobacter vinelandii mannuronan C-5-epimerase and alginate lyase AlgE7 probably originate from the same active site in the enzyme. J Biol Chem. 2001;276:31542-50 pubmed
    The Azotobacter vinelandii genome encodes a family of seven secreted Ca(2+)-dependent epimerases (AlgE1--7) catalyzing the polymer level epimerization of beta-D-mannuronic acid (M) to alpha-L-guluronic acid (G) in the commercially ..
  44. Fadeeva M, Núñez C, Bertsova Y, Espín G, Bogachev A. Catalytic properties of Na+-translocating NADH:quinone oxidoreductases from Vibrio harveyi, Klebsiella pneumoniae, and Azotobacter vinelandii. FEMS Microbiol Lett. 2008;279:116-23 pubmed
    ..the marine bacterium Vibrio harveyi, the enterobacterium Klebsiella pneumoniae, and the soil microorganism Azotobacter vinelandii have been comparatively analyzed...
  45. Hermawan S, Jendrossek D. Microscopical investigation of poly(3-hydroxybutyrate) granule formation in Azotobacter vinelandii. FEMS Microbiol Lett. 2007;266:60-4 pubmed
    Poly(3-hydroxybutyrate) (PHB) granule formation in Azotobacter vinelandii was investigated by laser scanning fluorescence microscopy after staining the cells with Nilered and Baclight...
  46. Hu Y, Fay A, Ribbe M. Identification of a nitrogenase FeMo cofactor precursor on NifEN complex. Proc Natl Acad Sci U S A. 2005;102:3236-41 pubmed
    The biosynthesis of the FeMo cofactor (FeMoco) of Azotobacter vinelandii nitrogenase presumably starts with the production of its Fe/S core by NifB (the nifB gene product)...
  47. Bollen Y, Kamphuis M, van Mierlo C. The folding energy landscape of apoflavodoxin is rugged: hydrogen exchange reveals nonproductive misfolded intermediates. Proc Natl Acad Sci U S A. 2006;103:4095-100 pubmed
    ..All PUFs detected thus are off the protein's productive folding route...
  48. Martinez Argudo I, Little R, Dixon R. A crucial arginine residue is required for a conformational switch in NifL to regulate nitrogen fixation in Azotobacter vinelandii. Proc Natl Acad Sci U S A. 2004;101:16316-21 pubmed
    NifL is an antiactivator that tightly regulates transcription of genes required for nitrogen fixation in Azotobacter vinelandii by controlling the activity of its partner protein NifA, a member of the family of sigma(54)-dependent ..
  49. Georgiadis M, Komiya H, Chakrabarti P, Woo D, Kornuc J, Rees D. Crystallographic structure of the nitrogenase iron protein from Azotobacter vinelandii. Science. 1992;257:1653-9 pubmed
    ..to address the role of ATP in nitrogen fixation, the crystal structure of the nitrogenase Fe-protein from Azotobacter vinelandii has been determined at 2.9 angstrom (A) resolution...
  50. Goodwin P, Agar J, Roll J, Roberts G, Johnson M, Dean D. The Azotobacter vinelandii NifEN complex contains two identical [4Fe-4S] clusters. Biochemistry. 1998;37:10420-8 pubmed
    The nifE and nifN gene products from Azotobacter vinelandii form an alpha2beta2 tetramer (NifEN complex) that is required for the biosynthesis of the nitrogenase FeMo cofactor...
  51. Hartmann M, Holm O, Johansen G, Skjak Braek G, Stokke B. Mode of action of recombinant Azotobacter vinelandii mannuronan C-5 epimerases AlgE2 and AlgE4. Biopolymers. 2002;63:77-88 pubmed
    ..The comparison between experimental data and simulation results suggests that epimerization by AlgE4 is best described by a processive mode of action, whereas the mode of action of AlgE2 appears to be more difficult to determine...
  52. Setubal J, dos Santos P, Goldman B, Ertesvåg H, Espin G, Rubio L, et al. Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes. J Bacteriol. 2009;191:4534-45 pubmed publisher
    b>Azotobacter vinelandii is a soil bacterium related to the Pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage...
  53. Frazzon J, Dean D. Biosynthesis of the nitrogenase iron-molybdenum-cofactor from Azotobacter vinelandii. Met Ions Biol Syst. 2002;39:163-86 pubmed
  54. Nabuurs S, de Kort B, Westphal A, van Mierlo C. Non-native hydrophobic interactions detected in unfolded apoflavodoxin by paramagnetic relaxation enhancement. Eur Biophys J. 2010;39:689-98 pubmed publisher
    ..Despite these difficulties, the spin-label data obtained here show that non-native contacts exist between transiently ordered structured elements in unfolded apoflavodoxin...
  55. Wang B, Pierson L, Rensing C, Gunatilaka M, Kennedy C. NasT-mediated antitermination plays an essential role in the regulation of the assimilatory nitrate reductase operon in Azotobacter vinelandii. Appl Environ Microbiol. 2012;78:6558-67 pubmed publisher
    b>Azotobacter vinelandii is a well-studied model system for nitrogen fixation in bacteria. Regulation of nitrogen fixation in A. vinelandii is independent of NtrB/NtrC, a conserved nitrogen regulatory system in proteobacteria...
  56. Sabra W, Zeng A, Deckwer W. Bacterial alginate: physiology, product quality and process aspects. Appl Microbiol Biotechnol. 2001;56:315-25 pubmed
    ..commercially produced from the marine brown algae, can also be biologically produced by bacteria such as Azotobacter vinelandii, A. chroococcum and several species of Pseudomonas...
  57. Curatti L, Brown C, Ludden P, Rubio L. Genes required for rapid expression of nitrogenase activity in Azotobacter vinelandii. Proc Natl Acad Sci U S A. 2005;102:6291-6 pubmed
    ..In this work, we investigate the role of rnf genes in the nitrogen-fixing bacterium Azotobacter vinelandii. We show that A...
  58. Funa N, Ozawa H, Hirata A, Horinouchi S. Phenolic lipid synthesis by type III polyketide synthases is essential for cyst formation in Azotobacter vinelandii. Proc Natl Acad Sci U S A. 2006;103:6356-61 pubmed
    Cysts of Azotobacter vinelandii are resting cells that are surrounded by a protective coat, conferring resistance to various chemical and physical agents...
  59. Spatzal T, Aksoyoglu M, Zhang L, Andrade S, Schleicher E, Weber S, et al. Evidence for interstitial carbon in nitrogenase FeMo cofactor. Science. 2011;334:940 pubmed publisher
    ..Atomic-resolution x-ray diffraction data and an electron spin echo envelope modulation (ESEEM) analysis now provide direct evidence that the ligand is a carbon species...
  60. Gaona G, Núñez C, Goldberg J, Linford A, Nájera R, Castañeda M, et al. Characterization of the Azotobacter vinelandii algC gene involved in alginate and lipopolysaccharide production. FEMS Microbiol Lett. 2004;238:199-206 pubmed
    b>Azotobacter vinelandii is a soil gamma-proteobacteria that fixes nitrogen and forms desiccation-resistant cysts. The exopolysaccharide alginate is an integral part of the layers surrounding the cysts. Here, we reported the cloning of A...
  61. Lu C, Stricker J, Erickson H. FtsZ from Escherichia coli, Azotobacter vinelandii, and Thermotoga maritima--quantitation, GTP hydrolysis, and assembly. Cell Motil Cytoskeleton. 1998;40:71-86 pubmed publisher
    We have cloned the ftsZ genes from Thermotoga maritima and Azotobacter vinelandii and expressed the proteins (TmFtsZ and AzFtsZ) in Escherichia coli. We compared these proteins to E...
  62. Chiu H, Peters J, Lanzilotta W, Ryle M, Seefeldt L, Howard J, et al. MgATP-Bound and nucleotide-free structures of a nitrogenase protein complex between the Leu 127 Delta-Fe-protein and the MoFe-protein. Biochemistry. 2001;40:641-50 pubmed
    ..The structure of this complex generated with proteins from Azotobacter vinelandii (designated the L127Delta-Av2-Av1 complex) has been crystallographically determined in the absence of ..
  63. van Mierlo C, van Dongen W, Vergeldt F, van Berkel W, Steensma E. The equilibrium unfolding of Azotobacter vinelandii apoflavodoxin II occurs via a relatively stable folding intermediate. Protein Sci. 1998;7:2331-44 pubmed
    A flavodoxin from Azotobacter vinelandii is chosen as a model system to study the folding of alpha/beta doubly wound proteins. The guanidinium hydrochloride induced unfolding of apoflavodoxin is demonstrated to be reversible...
  64. Perry S, Shearer N, Little R, Dixon R. Mutational analysis of the nucleotide-binding domain of the anti-activator NifL. J Mol Biol. 2005;346:935-49 pubmed
    The NifL regulatory protein controls transcription of nitrogen fixation genes in Azotobacter vinelandii by modulating the activity of the transcriptional activator NifA through direct protein-protein interactions...
  65. Little R, Colombo V, Leech A, Dixon R. Direct interaction of the NifL regulatory protein with the GlnK signal transducer enables the Azotobacter vinelandii NifL-NifA regulatory system to respond to conditions replete for nitrogen. J Biol Chem. 2002;277:15472-81 pubmed
    The Azotobacter vinelandii NifL-NifA regulatory system integrates metabolic signals for redox, energy, and nitrogen status to fine tune regulation of the synthesis of molybdenum nitrogenase...
  66. Pena C, Reyes C, Larralde Corona P, Corkidi G, Galindo E. Characterization of Azotobacter vinelandii aggregation in submerged culture by digital image analysis. FEMS Microbiol Lett. 2002;207:173-7 pubmed
    A simple and accurate method for determining the distribution of sizes of single cells and aggregates of Azotobacter vinelandii by image analysis has been developed...
  67. Belevich I, Borisov V, Bloch D, Konstantinov A, Verkhovsky M. Cytochrome bd from Azotobacter vinelandii: evidence for high-affinity oxygen binding. Biochemistry. 2007;46:11177-84 pubmed
    Cytochrome bd from Azotobacter vinelandii is a respiratory quinol oxidase that is highly efficient in reducing intracellular oxygen concentration, thus enabling nitrogen fixation under ambient aerobic conditions...
  68. Palanché T, Blanc S, Hennard C, Abdallah M, Albrecht Gary A. Bacterial iron transport: coordination properties of azotobactin, the highly fluorescent siderophore of Azotobacter vinelandii. Inorg Chem. 2004;43:1137-52 pubmed
    b>Azotobacter vinelandii, a nitrogen-fixing soil bacterium, secretes in iron deficiency azotobactin delta, a highly fluorescent pyoverdin-like chromopeptidic hexadentate siderophore...
  69. Key J, Hefti M, Purcell E, Moffat K. Structure of the redox sensor domain of Azotobacter vinelandii NifL at atomic resolution: signaling, dimerization, and mechanism. Biochemistry. 2007;46:3614-23 pubmed
    ..and transduction in NifL, we determined the crystal structure of the FAD-bound PAS domain of NifL from Azotobacter vinelandii to 1.04 A resolution...
  70. Jung Y, Kwon Y. Small RNA ArrF regulates the expression of sodB and feSII genes in Azotobacter vinelandii. Curr Microbiol. 2008;57:593-7 pubmed publisher
    b>Azotobacter vinelandii contains a prrF-like sequence in a noncoding region of the chromosome...
  71. Camba R, Jung Y, Hunsicker Wang L, Burgess B, Stout C, Hirst J, et al. Mechanisms of redox-coupled proton transfer in proteins: role of the proximal proline in reactions of the [3Fe-4S] cluster in Azotobacter vinelandii ferredoxin I. Biochemistry. 2003;42:10589-99 pubmed
    The 7Fe ferredoxin from Azotobacter vinelandii (AvFdI) contains a [3Fe-4S](+/0) cluster that binds a single proton in its reduced level...
  72. Page W, Kwon E, Cornish A, Tindale A. The csbX gene of Azotobacter vinelandii encodes an MFS efflux pump required for catecholate siderophore export. FEMS Microbiol Lett. 2003;228:211-6 pubmed
    The csbX gene of Azotobacter vinelandii was regulated in an iron-repressible manner from a divergent promoter upstream of the catecholate siderophore biosynthesis (csb) operon and was predicted to encode an efflux pump of the major ..
  73. Fisher K, Lowe D, Tavares P, Pereira A, Huynh B, Edmondson D, et al. Conformations generated during turnover of the Azotobacter vinelandii nitrogenase MoFe protein and their relationship to physiological function. J Inorg Biochem. 2007;101:1649-56 pubmed
    Various S=3/2 EPR signals elicited from wild-type and variant Azotobacter vinelandii nitrogenase MoFe proteins appear to reflect different conformations assumed by the FeMo-cofactor with different protonation states...
  74. Cocotl Yañez M, Sampieri A, Moreno S, Núñez C, Castañeda M, Segura D, et al. Roles of RpoS and PsrA in cyst formation and alkylresorcinol synthesis in Azotobacter vinelandii. Microbiology. 2011;157:1685-93 pubmed publisher
    b>Azotobacter vinelandii is a soil bacterium that undergoes differentiation to form cysts that are resistant to desiccation...
  75. Schlessman J, Woo D, Joshua Tor L, Howard J, Rees D. Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum. J Mol Biol. 1998;280:669-85 pubmed
    ..framework for addressing the diverse functions of Fe-protein, crystal structures of the Fe-proteins from Azotobacter vinelandii and Clostridium pasteurianum have been determined at resolutions of 2.2 A and 1.93 A, respectively...
  76. Pena C, Hernandez L, Galindo E. Manipulation of the acetylation degree of Azotobacter vinelandii alginate by supplementing the culture medium with 3-(N-morpholino)-propane-sulfonic acid. Lett Appl Microbiol. 2006;43:200-4 pubmed
    ..to characterize the influence of 3-(N-morpholino)-propane-sulfonic acid (MOPS) on alginate production by Azotobacter vinelandii and its chemical composition (particularly its acetylation degree), as well as on the rheological ..
  77. Aachmann F, Svanem B, Valla S, Petersen S, Wimmer R. NMR assignment of the R-module from the Azotobacter vinelandii Mannuronan C5-epimerase AlgE4. J Biomol NMR. 2005;31:259 pubmed
  78. Sandercock J, Page W. RpoS expression and the general stress response in Azotobacter vinelandii during carbon and nitrogen diauxic shifts. J Bacteriol. 2008;190:946-53 pubmed
    ..A mutant unable to produce RpoS was constructed using the diazotrophic bacterium Azotobacter vinelandii strain UW. Under nondesiccating, solid-medium growth conditions the wild type was culturable for 16...
  79. Mitra R, Das H, Dixit A. Identification of a positive transcription regulatory element within the coding region of the nifLA operon in Azotobacter vinelandii. Appl Environ Microbiol. 2005;71:3716-24 pubmed
    Nitrogen fixation in Azotobacter vinelandii is regulated by the nifLA operon...
  80. Beale J, Foster J. Carbohydrate fluxes into alginate biosynthesis in Azotobacter vinelandii NCIB 8789: NMR investigations of the triose pools. Biochemistry. 1996;35:4492-501 pubmed
    ..primary catabolism and into hexose resynthesis has been investigated for alginic acid biosynthesis in Azotobacter vinelandii. To do these studies, we fed the microorganism a variety of 13C-labeled glucose precursors, including [U-..