methylophilus methylotrophus

Summary

Summary: A species of METHYLOPHILUS which is motile by single flagella. In addition to growth on methanol as a sole carbon source, growth also occurs on glucose. (From Bergey's Manual of Determinative Bacteriology, 9th ed)

Top Publications

  1. Brennan L, Turner D, Fareleira P, Santos H. Solution structure of Methylophilus methylotrophus cytochrome c": insights into the structural basis of haem-ligand detachment. J Mol Biol. 2001;308:353-65 pubmed
    Cytochrome c" from Methylophilus methylotrophus is a monohaem protein with 124 amino acid residues. The iron has two histidine ligands in the oxidised form, one of which detaches and picks up a proton when the protein is reduced...
  2. Enguita F, Pohl E, Turner D, Santos H, Carrondo M. Structural evidence for a proton transfer pathway coupled with haem reduction of cytochrome c" from Methylophilus methylotrophus. J Biol Inorg Chem. 2006;11:189-96 pubmed
    The crystal structures of the oxidized and reduced forms of cytochrome c" from Methylophilus methylotrophus were solved from X-ray synchrotron data to atomic resolution...
  3. Jones M, Basran J, Sutcliffe M, G√ľnter Grossmann J, Scrutton N. X-ray scattering studies of Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein. Evidence for multiple conformational states and an induced fit mechanism for assembly with trimethylamine dehydrogenase. J Biol Chem. 2000;275:21349-54 pubmed
    ..model-independent molecular envelope structure for electron-transferring flavoprotein (ETF) from Methylophilus methylotrophus (sp. W(3)A(1))...
  4. Gunji Y, Yasueda H. Enhancement of L-lysine production in methylotroph Methylophilus methylotrophus by introducing a mutant LysE exporter. J Biotechnol. 2006;127:1-13 pubmed
    The obligate methylotroph Methylophilus methylotrophus AS1 expressing a mutant form of dapA (dapA24) encoding a dihydrodipicolinate synthase desensitized from feedback inhibition by L-lysine could secrete L-lysine into the medium, but ..
  5. Abalakina E, Tokmakova I, Gorshkova N, Gak E, Akhverdyan V, Mashko S, et al. Phage Mu-driven two-plasmid system for integration of recombinant DNA in the Methylophilus methylotrophus genome. Appl Microbiol Biotechnol. 2008;81:191-200 pubmed publisher
    ..A phage Mu-driven two-plasmid system for DNA integration in Escherichia coli genome has been adjusted for Methylophilus methylotrophus. Constructed helper plasmids with broad-host-range replicons carry thermo-inducible genes for ..
  6. Yomantas Y, Tokmakova I, Gorshkova N, Abalakina E, Kazakova S, Gak E, et al. Aromatic amino acid auxotrophs constructed by recombinant marker exchange in Methylophilus methylotrophus AS1 cells expressing the aroP-encoded transporter of Escherichia coli. Appl Environ Microbiol. 2010;76:75-83 pubmed publisher
    ..We describe a novel method of constructing mutants of the bacterium Methylophilus methylotrophus AS1 that are auxotrophic for aromatic amino acids...
  7. Nakonieczna J, Kaczorowski T, Obarska Kosinska A, Bujnicki J. Functional analysis of MmeI from methanol utilizer Methylophilus methylotrophus, a subtype IIC restriction-modification enzyme related to type I enzymes. Appl Environ Microbiol. 2009;75:212-23 pubmed publisher
    MmeI from Methylophilus methylotrophus belongs to the type II restriction-modification enzymes...
  8. Tsujimoto N, Gunji Y, Ogawa Miyata Y, Shimaoka M, Yasueda H. L-Lysine biosynthetic pathway of Methylophilus methylotrophus and construction of an L-lysine producer. J Biotechnol. 2006;124:327-37 pubmed
    ..synthase (DDPS), which are involved in L-lysine biosynthesis in the Gram-negative obligate methylotroph Methylophilus methylotrophus AS1, were inhibited by allosteric effectors, including L-lysine...
  9. Basran J, Chohan K, Sutcliffe M, Scrutton N. Differential coupling through Val-344 and Tyr-442 of trimethylamine dehydrogenase in electron transfer reactions with ferricenium ions and electron transferring flavoprotein. Biochemistry. 2000;39:9188-200 pubmed
    ..Mutation of Tyr-442 likely disrupts electron transfer by perturbing the interaction geometry of TMADH and ETF in the productive electron transfer complex, leading to less efficient coupling between the redox centers...

More Information

Publications32

  1. Schmitz M, Tinoco I. Solution structure and metal-ion binding of the P4 element from bacterial RNase P RNA. RNA. 2000;6:1212-25 pubmed
    ..The change of the metal-ion binding site provides a possible explanation for changes in catalytic activity of the mutant RNase P in the presence of Ca2+...
  2. Morgan R, Bhatia T, Lovasco L, Davis T. MmeI: a minimal Type II restriction-modification system that only modifies one DNA strand for host protection. Nucleic Acids Res. 2008;36:6558-70 pubmed publisher
    ..The MmeI architecture represents a minimal approach to assembling a restriction-modification system wherein a single DNA recognition domain targets both the endonuclease and DNA methyltransferase activities...
  3. Ishikawa K, Asahara T, Gunji Y, Yasueda H, Asano K. Disruption of metF increased L-lysine production by Methylophilus methylotrophus from methanol. Biosci Biotechnol Biochem. 2008;72:1317-24 pubmed
    Methionine auxotrophic mutants of Methylophilus methylotrophus AS1 expressing a mutant form of dapA (dapA24) encoding a dihydrodipicolinate synthase desensitized from feedback inhibition by L-lysine, and mutated lysE (lysE24) encoding the ..
  4. Enguita F, Rodrigues L, Archer M, Sieker L, Rodrigues A, Pohl E, et al. Crystallization and preliminary X-ray characterization of cytochrome c" from the obligate methylotroph Methylophilus methylotrophus. Acta Crystallogr D Biol Crystallogr. 2003;59:580-3 pubmed
    Cytochrome c" from the obligate methylotroph Methylophilus methylotrophus is a 15 kDa monohaem protein which has a c-type haem covalently linked to the protein chain...
  5. Burgess S, Messiha H, Katona G, Rigby S, Leys D, Scrutton N. Probing the dynamic interface between trimethylamine dehydrogenase (TMADH) and electron transferring flavoprotein (ETF) in the TMADH-2ETF complex: role of the Arg-alpha237 (ETF) and Tyr-442 (TMADH) residue pair. Biochemistry. 2008;47:5168-81 pubmed publisher
    ..Our work also points to the importance of Arg-alpha237 in controlling the thermodynamics of electron transfer, the dynamics of ETF, and the protection of reducing equivalents following disassembly of the TMADH-2ETF complex...
  6. Gunji Y, Ito H, Masaki H, Yasueda H. Characterization of a unique mutant lysE gene, originating from Corynebacterium glutamicum, encoding a product that induces L-lysine production in Methylophilus methylotrophus. Biosci Biotechnol Biochem. 2006;70:2927-34 pubmed
    ..The mutant gene is able to induce L-lysine production in Methylophilus methylotrophus. Although lysE24 has a mutation in the middle of lysE that results in chain termination, the entire ..
  7. Gunji Y, Tsujimoto N, Shimaoka M, Ogawa Miyata Y, Sugimoto S, Yasueda H. Characterization of the L-lysine biosynthetic pathway in the obligate methylotroph Methylophilus methylotrophus. Biosci Biotechnol Biochem. 2004;68:1449-60 pubmed
    The L-lysine biosynthetic pathway of the gram-negative obligate methylotroph Methylophilus methylotrophus AS1 was examined through characterization of the enzymes aspartokinase (AK), aspartsemialdehyde dehydrogenase, dihydrodipicolinate ..
  8. Ishikawa K, Toda Murakoshi Y, Ohnishi F, Kondo K, Osumi T, Asano K. Medium composition suitable for L-lysine production by Methylophilus methylotrophus in fed-batch cultivation. J Biosci Bioeng. 2008;106:574-9 pubmed publisher
    L-Lysine production was investigated in fed-batch fermentation using L-lysine producer of Methylophilus methylotrophus. By the addition of nutrient composition, containing L-methionine, K(2)HPO(4), NaH(2)PO(4), CuSO(4).5aq, MnSO(4)...
  9. Akhverdyan V, Gak E, Tokmakova I, Stoynova N, Yomantas Y, Mashko S. Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria--mini review. Appl Microbiol Biotechnol. 2011;91:857-71 pubmed publisher
    ..for the construction of stably maintained mini-Mu multi-integrant strains of Escherichia coli and Methylophilus methylotrophus are described...
  10. Hothi P, Sutcliffe M, Scrutton N. Kinetic isotope effects and ligand binding in PQQ-dependent methanol dehydrogenase. Biochem J. 2005;388:123-33 pubmed
    ..reaction of PQQ (2,7,9-tricarboxypyrroloquinoline quinone)-dependent MDH (methanol dehydrogenase) from Methylophilus methylotrophus has been studied under steady-state conditions in the presence of an alternative activator [GEE (..
  11. Shi W, Mersfelder J, Hille R. The interaction of trimethylamine dehydrogenase and electron-transferring flavoprotein. J Biol Chem. 2005;280:20239-46 pubmed
    ..partners trimethylamine dehydrogenase (TMADH) and electron-transferring flavoprotein (ETF) from Methylophilus methylotrophus has been examined with particular regard to the proposal that the former protein "imprints" a ..
  12. Lomtev A, Bobrov A, Vekshin N. [The release of flavin adenine dinucleotide upon local conformational transition in electron-transferring flavoprotein induced by trimethylamine dehydrogenase]. Bioorg Khim. 2004;30:247-53 pubmed
    ..A fluorescence method was proposed for distinguishing FAD from FMN in solution using ethylene glycol. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 3; see also http://www.maik.ru...
  13. Lu X, Nikolic D, Mitchell D, van Breemen R, Mersfelder J, Hille R, et al. A mechanism for substrate-Induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase. Bioorg Med Chem Lett. 2003;13:4129-32 pubmed
    ..The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H(2)(18)O and 18O(2), and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water...
  14. Leys D, Basran J, Talfournier F, Sutcliffe M, Scrutton N. Extensive conformational sampling in a ternary electron transfer complex. Nat Struct Biol. 2003;10:219-25 pubmed
    ..iron-sulfur flavoprotein trimethylamine dehydrogenase and electron transferring flavoprotein (ETF) from Methylophilus methylotrophus. In addition, we report the crystal structure of free ETF...
  15. Price N, Brennan L, Faria T, Vijgenboom E, Canters G, Turner D, et al. High yield of Methylophilus methylotrophus cytochrome c by coexpression with cytochrome c maturation gene cluster from Escherichia coli. Protein Expr Purif. 2000;20:444-50 pubmed
    ..We have expressed cytochrome c from Methylophilus methylotrophus in E...
  16. Indiani C, De Sanctis G, Neri F, Santos H, Smulevich G, Coletta M. Effect of pH on axial ligand coordination of cytochrome c" from Methylophilus methylotrophus and horse heart cytochrome c. Biochemistry. 2000;39:8234-42 pubmed
    ..the axial ligand coordination and on structural aspects of the protein moiety of cytochrome c' ' from Methylophilus methylotrophus, an obligate methylotroph, has been investigated down to very low pH (i.e., 0.3)...
  17. Trickey P, Basran J, Lian L, Chen Z, Barton J, Sutcliffe M, et al. Structural and biochemical characterization of recombinant wild type and a C30A mutant of trimethylamine dehydrogenase from methylophilus methylotrophus (sp. W(3)A(1)). Biochemistry. 2000;39:7678-88 pubmed
  18. Hothi P, Basran J, Sutcliffe M, Scrutton N. Effects of multiple ligand binding on kinetic isotope effects in PQQ-dependent methanol dehydrogenase. Biochemistry. 2003;42:3966-78 pubmed
    The reaction of PQQ-dependent methanol dehydrogenase (MDH) from Methylophilus methylotrophus has been studied by steady-state and stopped-flow kinetic methods, with particular reference to multiple ligand binding and the kinetic isotope ..
  19. Talfournier F, Munro A, Basran J, Sutcliffe M, Daff S, Chapman S, et al. alpha Arg-237 in Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein affords approximately 200-millivolt stabilization of the FAD anionic semiquinone and a kinetic block on full reduction to the dihydroquinone. J Biol Chem. 2001;276:20190-6 pubmed
    The midpoint reduction potentials of the FAD cofactor in wild-type Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein (ETF) and the alphaR237A mutant were determined by anaerobic redox titration...
  20. Ishikawa K, Gunji Y, Yasueda H, Asano K. Improvement of L-lysine production by Methylophilus methylotrophus from methanol via the Entner-Doudoroff pathway, originating in Escherichia coli. Biosci Biotechnol Biochem. 2008;72:2535-42 pubmed
    ..The metabolic response of Methylophilus methylotrophus upon the addition of glucose and of pyruvate was investigated in batch cultivation...
  21. Anderson R, Jang M, Hille R. Radiolytic studies of trimethylamine dehydrogenase. Spectral deconvolution of the neutral and anionic flavin semiquinone, and determination of rate constants for electron transfer in the one-electron reduced enzyme. J Biol Chem. 2000;275:30781-6 pubmed
    Trimethylamine dehydrogenase from the pseudomonad Methylophilus methylotrophus has been examined using the technique of pulse radiolysis to rapidly introduce a single reducing equivalent into the enzyme...
  22. Quintas P, Catarino T, Todorovic S, Turner D. Highly selective ligand binding by Methylophilus methylotrophus cytochrome c''. Biochemistry. 2011;50:5624-32 pubmed publisher
    Cytochrome c'' (cyt c'') from Methylophilus methylotrophus is unusual insofar as the heme has two axial histidine ligands in the oxidized form but one is detached when the protein is reduced...
  23. Jang M, Scrutton N, Hille R. Formation of W(3)A(1) electron-transferring flavoprotein (ETF) hydroquinone in the trimethylamine dehydrogenase x ETF protein complex. J Biol Chem. 2000;275:12546-52 pubmed
    The electron-transferring flavoprotein (ETF) from Methylophilus methylotrophus (sp...