Summary: A genus of straight or slightly curved gram-negative rods occurring singly or in pairs and isolated from sludge, mud, and river and pond water. (From Bergey's Manual of Determinative Bacteriology, 9th ed)

Top Publications

  1. Xia F, Zou B, Shen C, Zhu T, Gao X, Quan Z. Complete genome sequence of Methylophilus sp. TWE2 isolated from methane oxidation enrichment culture of tap-water. J Biotechnol. 2015;211:121-2 pubmed publisher
    The non-methane-utilizing methylotroph, Methylophilus sp. TWE2, was isolated from tap-water during the enrichment of methanotrophs with methane...
  2. Khan M, Lee J, Yoo K, Kim S, Park J. Improved TNT detoxification by starch addition in a nitrogen-fixing Methylophilus-dominant aerobic microbial consortium. J Hazard Mater. 2015;300:873-881 pubmed publisher
    ..Pyrosequencing targeting the bacterial 16S rRNA gene suggested that Methylophilus and Pseudoxanthomonas population were significantly stimulated by the co-addition of starch and TNT and that ..
  3. Gao X, Shao M, Wang Q, Wang L, Fang W, Ouyang F, et al. Airborne microbial communities in the atmospheric environment of urban hospitals in China. J Hazard Mater. 2018;349:10-17 pubmed publisher
    ..suggested that the dominant microorganisms at the genus level were Massilia, Sphingomonas, Methylobacterium, Methylophilus, Micrococcineae, and Corynebacterineae...
  4. Jiang H, Duan C, Luo M, Xing X. Enrichment and characteristics of mixed methane-oxidizing bacteria from a Chinese coal mine. Appl Microbiol Biotechnol. 2016;100:10331-10341 pubmed stable, Methylomonas and Methylocystis became the dominant type I and type II methanotrophs, while Methylophilus was the prevailing methylotroph...
  5. Chen R, Luo Y, Chen J, Zhang Y, Wen L, Shi L, et al. Evolution of the microbial community of the biofilm in a methane-based membrane biofilm reactor reducing multiple electron acceptors. Environ Sci Pollut Res Int. 2016;23:9540-8 pubmed publisher
    ..the methanotrophs became important, methanol-oxidizing denitrifying bacteria, namely, Methloversatilis and Methylophilus, became important in the biofilm, probably by utilizing organic matter generated by the metabolism of ..
  6. Wei X, He R, Chen M, Su Y, Ma R. Conversion of methane-derived carbon and microbial community in enrichment cultures in response to O2 availability. Environ Sci Pollut Res Int. 2016;23:7517-28 pubmed publisher
    ..Methylotrophs, mainly including Methylophilus, Methylovorus, Hyphomicrobium, and Methylobacillus, were also abundant in the cultures...
  7. Kim S, Rossmassler K, Broeckling C, Galloway S, Prenni J, De Long S. Impact of inoculum sources on biotransformation of pharmaceuticals and personal care products. Water Res. 2017;125:227-236 pubmed publisher
    ..Sphingomonas, Beijerinckia, Methylophilus, and unknown Cytophagaceae were linked with successful PPCP biodegradation via next-generation sequencing of ..
  8. Romdhane S, Devers Lamrani M, Martin Laurent F, Jrad A, Raviglione D, Salvia M, et al. Evidence for photolytic and microbial degradation processes in the dissipation of leptospermone, a natural β-triketone herbicide. Environ Sci Pollut Res Int. 2017;: pubmed publisher
    ..Based on its 16S ribosomal RNA (rRNA) gene sequence, it was affiliated to the Methylophilus group and was accordingly named as Methylophilus sp. LS1...
  9. Ma J, Zhang W, Zhang S, Zhu Q, Feng Q, Chen F. Short-term effects of CO2 leakage on the soil bacterial community in a simulated gas leakage scenario. Peerj. 2017;5:e4024 pubmed publisher
    ..64% to 9.29% (p < 0.01). Moreover, the abundances of genera Methylophilus, Methylobacillus, and Methylovorus increased, while GP4, GP6 and GP7 ..

More Information


  1. Li J, Gan J, Mathews F, Xia Z. The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase. Biochem Biophys Res Commun. 2011;406:621-6 pubmed publisher
    ..The crystal structure of methanol dehydrogenase from Methylophilus W3A1, previously determined at high resolution, exhibits a non-planar configuration of the PQQ ring system and ..
  2. Douterelo I, Sharpe R, Boxall J. Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system. Water Res. 2013;47:503-16 pubmed publisher
    ..This research brings new knowledge regarding the influence of different hydraulic regimes on the composition and structure of bacterial communities within DWDS and the implication that this might have on drinking water quality...
  3. Madhaiyan M, Poonguzhali S, Kwon S, Sa T. Methylophilus rhizosphaerae sp. nov., a restricted facultative methylotroph isolated from rice rhizosphere soil. Int J Syst Evol Microbiol. 2009;59:2904-8 pubmed publisher
    ..Phylogenetic analysis based on the 16S rRNA and methanol dehydrogenase (mxaF) gene sequences showed that Methylophilus methylotrophus was their close relative...
  4. Pshenichnikova A, Nevo A, Volkova E, Skladnev D, Shvets V. [Effect of deuteration on methanol dehydrogenase activity in Methylophilus sp. B-7741]. Prikl Biokhim Mikrobiol. 2004;40:24-7 pubmed
    ..1.99.8) from methylotrophic bacteria Methylophilus sp. B-7741...
  5. Cai B, Vuilleumier S, Wackett L. [Site-directed mutagenesis of the dichloromethane dehalogenase gene from Methylophilus sp. strain DM11]. Wei Sheng Wu Xue Bao. 1998;38:163-7 pubmed
    In order to investigate the role of different residues of Methylophilus sp...
  6. Vuilleumier S, Ivos N, Dean M, Leisinger T. Sequence variation in dichloromethane dehalogenases/glutathione S-transferases. Microbiology. 2001;147:611-9 pubmed
    ..of the dcmA gene encoding DCM dehalogenase has been determined for Methylobacterium dichloromethanicum DM4 and Methylophilus sp. DM11...
  7. Choi S, Cho M, Ahn S, Lee J, Lee J, Kim D, et al. Endosymbionts of Acanthamoeba isolated from domestic tap water in Korea. Korean J Parasitol. 2009;47:337-44 pubmed publisher
    ..The endosymbiont of Acanthamoeba sp. KA/WP9 exhibits a high degree of similarity (85-95%) with genus Methylophilus, which is not yet known to harbor any endosymbionts...
  8. Gogleva A, Kaparullina E, Doronina N, Trotsenko Y. Methylophilus flavus sp. nov. and Methylophilus luteus sp. nov., aerobic, methylotrophic bacteria associated with plants. Int J Syst Evol Microbiol. 2010;60:2623-8 pubmed publisher
    ..analysis and low levels of DNA-DNA relatedness with the type strains of recognized species of the genus Methylophilus (31-36?%), strains Ship(T) and Mim(T) are considered to represent novel species of the genus Methylophilus, ..
  9. Schmitz U, Behrens S, Freymann D, Keenan R, Lukavsky P, Walter P, et al. Structure of the phylogenetically most conserved domain of SRP RNA. RNA. 1999;5:1419-29 pubmed
    ..The structure of domain IV suggests that loop A is the initial site for the RNA/protein interaction creating specificity, whereas loop B provides a secondary interaction site...
  10. Doronina N, Gogleva A, Trotsenko Y. Methylophilus glucosoxydans sp. nov., a restricted facultative methylotroph from rice rhizosphere. Int J Syst Evol Microbiol. 2012;62:196-201 pubmed publisher
    ..and methanol dehydrogenase (mxaF) gene sequences showed a high level of similarity to members of the genus Methylophilus. As the two isolates were clearly distinct from all recognized members of the genus Methylophilus based on ..
  11. Zhang S, Wang Q, Wan R, Xie S. Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil. J Zhejiang Univ Sci B. 2011;12:760-8 pubmed publisher
    ..Genera Methylophilus, Mesorhizobium, and Terrimonas had potential links to anthracene biodegradation, suggesting a consortium ..
  12. Giri D, Kumar A, Shukla P, Singh R, Singh P, Pandey K. Salt stress tolerance of methylotrophic bacteria Methylophilus sp. and Methylobacterium sp. isolated from coal mine spoils. Pol J Microbiol. 2013;62:273-80 pubmed
    ..of Bina coalmine spoil BNV7b and BRV25 were identified based on physiological traits and 16S rDNA sequence as Methylophilus and Methylobacterium species...
  13. Noronha M, Santos R, Paci E, Santos H, Maçanita A. Fluorescence lifetimes of tyrosine residues in cytochrome c'' as local probes to study protein unfolding. J Phys Chem B. 2009;113:4466-74 pubmed publisher
    ..Cytochrome c'' from Methylophilus methylotrophus, which has four tyrosine residues, was chosen as a model protein...
  14. Doronina N, Ivanova E, Trotsenko Y, Pshenichnikova A, Kalinina E, Shvets V. Methylophilus quaylei sp. nov., a new aerobic obligately methylotrophic bacterium. Syst Appl Microbiol. 2005;28:303-9 pubmed
    ..0 mol%. Based on 16S rDNA sequence analysis and DNA-DNA relatedness (29-34%) with type strains of the genus Methylophilus, the novel isolate was classified as a new species of this genus and named Methylophilus quaylei MTT (VKM B-..
  15. Reshetnikov A, Khmelenina V, Trotsenko Y. Detection of ectoin biosynthesis genes in halotolerant aerobic methylotrophic bacteria. Dokl Biochem Biophys. 2004;396:200-2 pubmed
  16. Stourman N, Rose J, Vuilleumier S, Armstrong R. Catalytic mechanism of dichloromethane dehalogenase from Methylophilus sp. strain DM11. Biochemistry. 2003;42:11048-56 pubmed
    The glutathione (GSH)-dependent dichloromethane dehalogenase from Methylophilus sp...
  17. Turova T, Kuznetsov B, Doronina N, Trotsenko I. [Phylogenetic analysis of aerobic methylotrophic bacteria, using dichloromethane]. Mikrobiologiia. 2001;70:92-7 pubmed
    ..The restricted facultative methylotroph "Methylophilus leisingerii" DM11 with the ribulose monophosphate pathway was found to belong to the genus Methylophilus ..
  18. Nevo A, Pshenichnikova A, Skladnev D, Shvets V. [Deuterium oxide as a stress factor to the methylotrophic bacterium Methylophilus sp]. Mikrobiologiia. 2004;73:175-9 pubmed
    The adaptation of the methylotrophic bacterium Methylophilus sp. B-7741 to growth in highly deuterated media was studied...