Summary: A family of gram-negative bacteria in the order Methylophilales.

Top Publications

  1. Kalyuzhnaya M, Bowerman S, Lara J, Lidstrom M, Chistoserdova L. Methylotenera mobilis gen. nov., sp. nov., an obligately methylamine-utilizing bacterium within the family Methylophilaceae. Int J Syst Evol Microbiol. 2006;56:2819-23 pubmed
    ..Betaproteobacteria, but only distantly related (93-96 % similarity) to known methylotrophs of the family Methylophilaceae. Strain JLW8(T) (=ATCC BAA-1282(T)=DSM 17540(T)) is proposed as the type strain of a novel species in a new ..
  2. Lapidus A, Clum A, LaButti K, Kaluzhnaya M, Lim S, Beck D, et al. Genomes of three methylotrophs from a single niche reveal the genetic and metabolic divergence of the methylophilaceae. J Bacteriol. 2011;193:3757-64 pubmed publisher
    The genomes of three representatives of the family Methylophilaceae, Methylotenera mobilis JLW8, Methylotenera versatilis 301, and Methylovorus glucosetrophus SIP3-4, all isolated from a single study site, Lake Washington in Seattle, WA, ..
  3. Karwautz C, Kus G, Stöckl M, Neu T, Lueders T. Microbial megacities fueled by methane oxidation in a mineral spring cave. ISME J. 2018;12:87-100 pubmed publisher
    ..of aerobic methanotrophs primarily within the Methylococcales (Gammaproteobacteria) and methylotrophic Methylophilaceae (Betaproteobacteria) were found in the biofilms, along with a surprising diversity of associated ..
  4. Olakanye A, Nelson A, Ralebitso Senior T. A comparative in situ decomposition study using still born piglets and leaf litter from a deciduous forest. Forensic Sci Int. 2017;276:85-92 pubmed publisher
    ..Furthermore, Methylophilaceae (Methylophilales order) and Anaerolineaceae would differentiate for the piglet and leaf litter soils, ..
  5. Rissanen A, Ojala A, Fred T, Toivonen J, Tiirola M. Methylophilaceae and Hyphomicrobium as target taxonomic groups in monitoring the function of methanol-fed denitrification biofilters in municipal wastewater treatment plants. J Ind Microbiol Biotechnol. 2017;44:35-47 pubmed publisher
    ..Combining the data with previous study results, the family Methylophilaceae and genus Hyphomicrobium were determined as suitable target groups for monitoring...
  6. Avilés Jiménez F, Güitrón A, Segura López F, Méndez Tenorio A, Iwai S, Hernández Guerrero A, et al. Microbiota studies in the bile duct strongly suggest a role for Helicobacter pylori in extrahepatic cholangiocarcinoma. Clin Microbiol Infect. 2016;22:178.e11-178.e22 pubmed publisher
    ..Among these, Nesterenkonia decreased, whereas Methylophilaceae, Fusobacterium, Prevotella, Actinomyces, Novosphingobium and H. pylori increased in ECCA...
  7. Zhang S, Zhou Z, Li Y, Meng F. Deciphering the core fouling-causing microbiota in a membrane bioreactor: Low abundance but important roles. Chemosphere. 2018;195:108-118 pubmed publisher
    ..Interestingly, a large proportion of the active biomarkers in bio-cake samples, such as Methylophilaceae, Burkholderiaceae, Paucibacter and Pseudoxanthomonas, did not fall within the abundant taxa (i.e., <0...
  8. Sanchez E, Bletz M, Duntsch L, Bhuju S, Geffers R, Jarek M, et al. Cutaneous Bacterial Communities of a Poisonous Salamander: a Perspective from Life Stages, Body Parts and Environmental Conditions. Microb Ecol. 2017;73:455-465 pubmed publisher
    ..Their bacterial community structure also differed; species of Burkholderiaceae, Comamonadaceae, Methylophilaceae and Sphingomonadaceae were more abundant in pond larvae, possibly related to differences in factors like ..
  9. Kalyuzhnaya M, Beck D, Suciu D, Pozhitkov A, Lidstrom M, Chistoserdova L. Functioning in situ: gene expression in Methylotenera mobilis in its native environment as assessed through transcriptomics. ISME J. 2010;4:388-98 pubmed publisher
    ..Overall, this study offers a novel approach for gaining insights into the lifestyle of individual microbes in their native environments...

More Information


  1. Doronina N, Ivanova E, Trotsenko Y. Phylogenetic position and emended description of the genus Methylovorus. Int J Syst Evol Microbiol. 2005;55:903-6 pubmed
  2. Yamauchi K, Sata M, Machiya J, Osaka D, Wada T, Abe S, et al. Antineutrophil cytoplasmic antibody positive alveolar haemorrhage during propylthiouracil therapy for hyperthyroidism. Respirology. 2003;8:532-5 pubmed
    ..Diminution and discontinuation of PTU resulted in a positive response. To our knowledge, this is the first documentation of both MPO- and PR3-ANCA-positive DAH, without systemic manifestations, developing during PTU therapy...
  3. Lueders T, Wagner B, Claus P, Friedrich M. Stable isotope probing of rRNA and DNA reveals a dynamic methylotroph community and trophic interactions with fungi and protozoa in oxic rice field soil. Environ Microbiol. 2004;6:60-72 pubmed
    ..of 'heavy' bacterial rRNA showed that mostly members of the Methylobacteriaceae and a novel clade within the Methylophilaceae formed part of the indigenous methylotrophic community...
  4. Bosch G, Wang T, Latypova E, Kalyuzhnaya M, Hackett M, Chistoserdova L. Insights into the physiology of Methylotenera mobilis as revealed by metagenome-based shotgun proteomic analysis. Microbiology. 2009;155:1103-10 pubmed publisher
    ..20 %) could be identified with high confidence (three or more peptide sequences), thus gaining insights into the physiology of this bacterium, which represents a new genus within the family Methylophilaceae.
  5. Kalyuzhnaya M, Lapidus A, Ivanova N, Copeland A, McHardy A, Szeto E, et al. High-resolution metagenomics targets specific functional types in complex microbial communities. Nat Biotechnol. 2008;26:1029-34 pubmed publisher
    ..This high-resolution, targeted metagenomics approach may be applicable to a wide variety of ecosystems...
  6. Beck D, Hendrickson E, Vorobev A, Wang T, Lim S, Kalyuzhnaya M, et al. An integrated proteomics/transcriptomics approach points to oxygen as the main electron sink for methanol metabolism in Methylotenera mobilis. J Bacteriol. 2011;193:4758-65 pubmed publisher
    ..This study identifies initial targets for future focused physiological studies, including mutant analysis, which will provide further details into this novel process...
  7. Mustakhimov I, Kalyuzhnaya M, Lidstrom M, Chistoserdova L. Insights into denitrification in Methylotenera mobilis from denitrification pathway and methanol metabolism mutants. J Bacteriol. 2013;195:2207-11 pubmed publisher
    ..mobilis JLW8. Reduced levels of nitrous oxide accumulated by the XoxF mutants compared to the wild type suggest that these enzymes must be capable of donating electrons for denitrification...
  8. Ge X, Wang W, Han Y, Wang J, Xiong X, Zhang W. Methylovorus sp. MP688 exopolysaccharides contribute to oxidative defense and bacterial survival under adverse condition. World J Microbiol Biotechnol. 2013;29:2249-58 pubmed publisher
    ..It was reasonable to conclude that EPS produced by Methylovorus sp. MP688 contributed to oxidative defense and bacterial survival under adverse condition. ..
  9. Yamamoto S, Morihara Y, Wakayama M, Tachiki T. Theanine production by coupled fermentation with energy transfer using gamma-glutamylmethylamide synthetase of Methylovorus mays No. 9. Biosci Biotechnol Biochem. 2008;72:1206-11 pubmed
    ..0), 30 mM MgCl(2), 5 mM MnCl(2), 5 mM AMP, 30 units/ml of GMAS, and 40 mg/ml of yeast cells. The yield of theanine was 100% on the substrates (glutamic acid and ethylamine) and also on the energy source (glucose consumed)...
  10. Jimenez Infante F, Ngugi D, Vinu M, Alam I, Kamau A, Blom J, et al. Comprehensive Genomic Analyses of the OM43 Clade, Including a Novel Species from the Red Sea, Indicate Ecotype Differentiation among Marine Methylotrophs. Appl Environ Microbiol. 2016;82:1215-1226 pubmed publisher
    The OM43 clade within the family Methylophilaceae of Betaproteobacteria represents a group of methylotrophs that play important roles in the metabolism of C1 compounds in marine environments and other aquatic environments around the globe...
  11. Xiong X, Zhi J, Yang L, Wang J, Zhao Y, Wang X, et al. Complete genome sequence of the bacterium Methylovorus sp. strain MP688, a high-level producer of pyrroloquinolone quinone. J Bacteriol. 2011;193:1012-3 pubmed publisher
    ..Here we report the finished, annotated genome sequence of the methylotrophic bacterium Methylovorus sp. strain MP688, which was isolated from soil for high-level production of pyrroloquinolone quinone (PQQ) in our lab...
  12. Kalyuzhnaya M, Beck D, Vorobev A, Smalley N, Kunkel D, Lidstrom M, et al. Novel methylotrophic isolates from lake sediment, description of Methylotenera versatilis sp. nov. and emended description of the genus Methylotenera. Int J Syst Evol Microbiol. 2012;62:106-11 pubmed publisher
    ..nov. is proposed; the type strain is 301(T) (=VKM B-2679(T)=JCM 17579(T)). An emended description of the genus Methylotenera is provided...
  13. Chistoserdova L. Methylotrophy in a lake: from metagenomics to single-organism physiology. Appl Environ Microbiol. 2011;77:4705-11 pubmed publisher
    ..Comparative analyses of individual genomes of Methylophilaceae from Lake Washington provide insights into their genomic divergence and suggest significant metabolic ..
  14. Eshinimaev B, Tsyrenzhapova I, Khmelenina V, Trotsenko I. [Detection of osmoprotector ectoine content in methylotrophic bacteria using of normal-phase high performance liquid chromatography]. Prikl Biokhim Mikrobiol. 2007;43:215-8 pubmed
  15. Kim K, Kim J, Kim D, Kim H, Park S, Kim Y. Molecular cloning of the DNA gyrase genes from Methylovorus sp. strain SS1 and the mechanism of intrinsic quinolone resistance in methylotrophic bacteria. Mol Cells. 2005;20:392-400 pubmed