methylobacterium extorquens

Summary

Summary: A species of METHYLOBACTERIUM which can utilize acetate, ethanol, or methylamine as a sole carbon source. (From Bergey's Manual of Determinative Bacteriology, 9th ed)

Top Publications

  1. Vorholt J, Marx C, Lidstrom M, Thauer R. Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol. J Bacteriol. 2000;182:6645-50 pubmed
    ..In the alpha-proteobacterium Methylobacterium extorquens AM1, we found a previously unknown enzyme that efficiently catalyzes the removal of formaldehyde: it ..
  2. Chou H, Berthet J, Marx C. Fast growth increases the selective advantage of a mutation arising recurrently during evolution under metal limitation. PLoS Genet. 2009;5:e1000652 pubmed publisher
    ..the genetic and physiological basis of adaptation to metal limitation using experimental populations of Methylobacterium extorquens AM1 evolved in metal-deficient growth media...
  3. Marx C, Chistoserdova L, Lidstrom M. Formaldehyde-detoxifying role of the tetrahydromethanopterin-linked pathway in Methylobacterium extorquens AM1. J Bacteriol. 2003;185:7160-8 pubmed
    The facultative methylotroph Methylobacterium extorquens AM1 possesses two pterin-dependent pathways for C(1) transfer between formaldehyde and formate, the tetrahydrofolate (H(4)F)-linked pathway and the tetrahydromethanopterin (H(4)MPT)..
  4. Marx C, Lidstrom M. Development of an insertional expression vector system for Methylobacterium extorquens AM1 and generation of null mutants lacking mtdA and/or fch. Microbiology. 2004;150:9-19 pubmed
    Over the past few years, the genetic 'toolkit' available for use with Methylobacterium extorquens AM1 has expanded significantly...
  5. Guo X, Lidstrom M. Physiological analysis of Methylobacterium extorquens AM1 grown in continuous and batch cultures. Arch Microbiol. 2006;186:139-49 pubmed
    Chemostat cultures of Methylobacterium extorquens AM1 grown on methanol or succinate at a range of dilution rates were compared to batch cultures in terms of enzyme levels, poly-beta-hydroxybutyrate content, and intracellular ..
  6. Chou H, Chiu H, Delaney N, Segrè D, Marx C. Diminishing returns epistasis among beneficial mutations decelerates adaptation. Science. 2011;332:1190-2 pubmed publisher
  7. Korotkova N, Chistoserdova L, Lidstrom M. Poly-beta-hydroxybutyrate biosynthesis in the facultative methylotroph methylobacterium extorquens AM1: identification and mutation of gap11, gap20, and phaR. J Bacteriol. 2002;184:6174-81 pubmed
    b>Methylobacterium extorquens AM1, a serine cycle facultative methylotroph, accumulates poly-beta-hydroxybutyrate (PHB) as a carbon and energy reserve material during growth on both multicarbon- and single-carbon substrates...
  8. Marx C. Recovering from a bad start: rapid adaptation and tradeoffs to growth below a threshold density. BMC Evol Biol. 2012;12:109 pubmed publisher
    ..Here I report the unexpected discovery of a density threshold for growth of a strain of Methylobacterium extorquens AM1 used to inoculate eight replicate populations that were evolved in methanol...
  9. Chubiz L, Purswani J, Carroll S, Marx C. A novel pair of inducible expression vectors for use in Methylobacterium extorquens. BMC Res Notes. 2013;6:183 pubmed publisher
    ..end, we have sought to develop a pair of inducible expression vectors for use in the ?-proteobacterium Methylobacterium extorquens, a model methylotroph. We found that the P(R) promoter from rhizobial phage 16-3 was active in M...

More Information

Publications71

  1. Marx C, O Brien B, Breezee J, Lidstrom M. Novel methylotrophy genes of Methylobacterium extorquens AM1 identified by using transposon mutagenesis including a putative dihydromethanopterin reductase. J Bacteriol. 2003;185:669-73 pubmed
    Ten novel methylotrophy genes of the facultative methylotroph Methylobacterium extorquens AM1 were identified from a transposon mutagenesis screen. One of these genes encodes a product having identity with dihydrofolate reductase (DHFR)...
  2. Crowther G, Kosály G, Lidstrom M. Formate as the main branch point for methylotrophic metabolism in Methylobacterium extorquens AM1. J Bacteriol. 2008;190:5057-62 pubmed publisher
    ..This problem was investigated in Methylobacterium extorquens AM1...
  3. Van Dien S, Okubo Y, Hough M, Korotkova N, Taitano T, Lidstrom M. Reconstruction of C(3) and C(4) metabolism in Methylobacterium extorquens AM1 using transposon mutagenesis. Microbiology. 2003;149:601-9 pubmed
    The growth of Methylobacterium extorquens AM1 on C(1) compounds has been well-studied, but little is known about how this methylotroph grows on multicarbon compounds...
  4. Penalver C, Cantet F, Morin D, Haras D, Vorholt J. A plasmid-borne truncated luxI homolog controls quorum-sensing systems and extracellular carbohydrate production in Methylobacterium extorquens AM1. J Bacteriol. 2006;188:7321-4 pubmed
    A cryptic plasmid of Methylobacterium extorquens AM1 was found to encode tslI, a truncated luxI homolog...
  5. Erb T, Berg I, Brecht V, Muller M, Fuchs G, Alber B. Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway. Proc Natl Acad Sci U S A. 2007;104:10631-6 pubmed
    ..For methylotrophic bacteria such as Methylobacterium extorquens, extension of the serine cycle with reactions of the ethylmalonyl-CoA pathway leads to a simplified ..
  6. Lee M, Marx C. Repeated, selection-driven genome reduction of accessory genes in experimental populations. PLoS Genet. 2012;8:e1002651 pubmed publisher
    ..In just 1,500 generations of experimental evolution, 80% of populations of Methylobacterium extorquens AM1 experienced nearly parallel deletions removing up to 10% of the genome from a megaplasmid present ..
  7. Schmidt S, Christen P, Kiefer P, Vorholt J. Functional investigation of methanol dehydrogenase-like protein XoxF in Methylobacterium extorquens AM1. Microbiology. 2010;156:2575-86 pubmed publisher
    Methanol dehydrogenase-like protein XoxF of Methylobacterium extorquens AM1 exhibits a sequence identity of 50 % to the catalytic subunit MxaF of periplasmic methanol dehydrogenase in the same organism...
  8. Strovas T, Sauter L, Guo X, Lidstrom M. Cell-to-cell heterogeneity in growth rate and gene expression in Methylobacterium extorquens AM1. J Bacteriol. 2007;189:7127-33 pubmed
    ..heterogeneity in gene expression and growth parameters was assessed in the facultative methylotroph Methylobacterium extorquens AM1...
  9. Hu B, Lidstrom M. CcrR, a TetR family transcriptional regulator, activates the transcription of a gene of the Ethylmalonyl coenzyme A pathway in Methylobacterium extorquens AM1. J Bacteriol. 2012;194:2802-8 pubmed publisher
    The ethylmalonyl coenzyme A (ethylmalonyl-CoA) pathway is one of the central methylotrophy pathways in Methylobacterium extorquens involved in glyoxylate generation and acetyl-CoA assimilation...
  10. Guo X, Lidstrom M. Metabolite profiling analysis of Methylobacterium extorquens AM1 by comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry. Biotechnol Bioeng. 2008;99:929-40 pubmed
    b>Methylobacterium extorquens AM1 is a facultative methylotroph, which is a potential candidate to be used in commercial processes to convert simple one-carbon compounds to a variety of multicarbon chemicals and products...
  11. Bergmiller T, Ackermann M. Pole age affects cell size and the timing of cell division in Methylobacterium extorquens AM1. J Bacteriol. 2011;193:5216-21 pubmed publisher
    ..Working with the alphaproteobacterium Methylobacterium extorquens, we monitored individuals in clonally growing populations over several divisions and determined the ..
  12. Pomper B, Saurel O, Milon A, Vorholt J. Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1. FEBS Lett. 2002;523:133-7 pubmed
    b>Methylobacterium extorquens AM1 possesses a formyltransferase (Ftr) complex that is essential for growth in the presence of methanol and involved in formaldehyde oxidation to CO(2)...
  13. Chistoserdova L, Crowther G, Vorholt J, Skovran E, Portais J, Lidstrom M. Identification of a fourth formate dehydrogenase in Methylobacterium extorquens AM1 and confirmation of the essential role of formate oxidation in methylotrophy. J Bacteriol. 2007;189:9076-81 pubmed
    A mutant of Methylobacterium extorquens AM1 with lesions in genes for three formate dehydrogenase (FDH) enzymes was previously described by us (L. Chistoserdova, M. Laukel, J.-C. Portais, J. A. Vorholt, and M. E. Lidstrom, J. Bacteriol...
  14. Fedorov D, Doronina N, Trotsenko Y. Cloning and characterization of indolepyruvate decarboxylase from Methylobacterium extorquens AM1. Biochemistry (Mosc). 2010;75:1435-43 pubmed
    ..An open reading frame (ORF) was identified in the genome of facultative methylotroph Methylobacterium extorquens AM1 using BLAST...
  15. Skovran E, Palmer A, Rountree A, Good N, Lidstrom M. XoxF is required for expression of methanol dehydrogenase in Methylobacterium extorquens AM1. J Bacteriol. 2011;193:6032-8 pubmed publisher
    ..The Methylobacterium extorquens AM1 genome contains two homologs of MxaF, XoxF1 and XoxF2, which are ?50% identical to MxaF and ?90% ..
  16. Korotkova N, Lidstrom M. MeaB is a component of the methylmalonyl-CoA mutase complex required for protection of the enzyme from inactivation. J Biol Chem. 2004;279:13652-8 pubmed
    ..Sci. U. S. A. 99, 15554-15559). MMAA orthologs are widespread in bacteria, archaea, and eukaryotes. In Methylobacterium extorquens AM1, a mutant defective in the MMAA homolog meaB was unable to grow on C(1) and C(2) compounds because ..
  17. Peyraud R, Schneider K, Kiefer P, Massou S, Vorholt J, Portais J. Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquens AM1. BMC Syst Biol. 2011;5:189 pubmed publisher
  18. Schneider K, Peyraud R, Kiefer P, Christen P, Delmotte N, Massou S, et al. The ethylmalonyl-CoA pathway is used in place of the glyoxylate cycle by Methylobacterium extorquens AM1 during growth on acetate. J Biol Chem. 2012;287:757-66 pubmed publisher
    ..In this study, we analyzed the metabolism of the facultative methylotroph Methylobacterium extorquens AM1, which lacks isocitrate lyase, the key enzyme in the glyoxylate cycle, during growth on acetate...
  19. Van Dien S, Marx C, O Brien B, Lidstrom M. Genetic characterization of the carotenoid biosynthetic pathway in Methylobacterium extorquens AM1 and isolation of a colorless mutant. Appl Environ Microbiol. 2003;69:7563-6 pubmed
    ..reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph Methylobacterium extorquens AM1. Four genes for putative phytoene desaturases were identified...
  20. Schneider K, Skovran E, Vorholt J. Oxalyl-coenzyme A reduction to glyoxylate is the preferred route of oxalate assimilation in Methylobacterium extorquens AM1. J Bacteriol. 2012;194:3144-55 pubmed publisher
    Oxalate catabolism is conducted by phylogenetically diverse organisms, including Methylobacterium extorquens AM1...
  21. Marx C, Lidstrom M. Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Microbiology. 2001;147:2065-75 pubmed
    ..One bacterium for which new genetic tools are needed is the methylotroph Methylobacterium extorquens AM1...
  22. Yang S, Sadilek M, Synovec R, Lidstrom M. Liquid chromatography-tandem quadrupole mass spectrometry and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry measurement of targeted metabolites of Methylobacterium extorquens AM1 grown on two different carbon sourc. J Chromatogr A. 2009;1216:3280-9 pubmed publisher
    ..A (ethylmalonyl-CoA) pathway and poly-beta-hydroxybutyrate cycle] of the bacterium Methylobacterium extorquens AM1 grown on two carbon sources, ethylamine (C2) and succinate (C4)...
  23. Strovas T, Lidstrom M. Population heterogeneity in Methylobacterium extorquens AM1. Microbiology. 2009;155:2040-8 pubmed publisher
    ..within exponentially growing populations was addressed in a bacterium, the facultative methylotroph Methylobacterium extorquens AM1...
  24. Abanda Nkpwatt D, Müsch M, Tschiersch J, Boettner M, Schwab W. Molecular interaction between Methylobacterium extorquens and seedlings: growth promotion, methanol consumption, and localization of the methanol emission site. J Exp Bot. 2006;57:4025-32 pubmed
    Four Methylobacterium extorquens strains were isolated from strawberry (Fragaria x ananassa cv. Elsanta) leaves, and one strain, called ME4, was tested for its ability to promote the growth of various plant seedlings...
  25. Kalyuzhnaya M, Korotkova N, Crowther G, Marx C, Lidstrom M, Chistoserdova L. Analysis of gene islands involved in methanopterin-linked C1 transfer reactions reveals new functions and provides evolutionary insights. J Bacteriol. 2005;187:4607-14 pubmed
  26. Nieto Penalver C, Morin D, Cantet F, Saurel O, Milon A, Vorholt J. Methylobacterium extorquens AM1 produces a novel type of acyl-homoserine lactone with a double unsaturated side chain under methylotrophic growth conditions. FEBS Lett. 2006;580:561-7 pubmed
    ..We have found that Methylobacterium extorquens AM1, a member of the pink-pigmented facultative methylotrophs commonly present on plant surfaces, ..
  27. Kato Y, Asahara M, Arai D, Goto K, Yokota A. Reclassification of Methylobacterium chloromethanicum and Methylobacterium dichloromethanicum as later subjective synonyms of Methylobacterium extorquens and of Methylobacterium lusitanum as a later subjective synonym of Methylobacterium rhodesianum. J Gen Appl Microbiol. 2005;51:287-99 pubmed
    ..According to these results, many Methylobacterium strains should be reclassified, with M. dichloromethanicum and M. chloromethanicum regarded as a synonym of M. extorquens, and M. lusitanum a synonym for M. rhodesianum...
  28. Okubo Y, Yang S, Chistoserdova L, Lidstrom M. Alternative route for glyoxylate consumption during growth on two-carbon compounds by Methylobacterium extorquens AM1. J Bacteriol. 2010;192:1813-23 pubmed publisher
    b>Methylobacterium extorquens AM1 is a facultative methylotroph capable of growth on both single-carbon and multicarbon compounds...
  29. Sy A, Timmers A, Knief C, Vorholt J. Methylotrophic metabolism is advantageous for Methylobacterium extorquens during colonization of Medicago truncatula under competitive conditions. Appl Environ Microbiol. 2005;71:7245-52 pubmed
    ..Competition experiments with Methylobacterium extorquens wild-type strain AM1 and methylotrophy mutants revealed that the ability to use methanol as a carbon ..
  30. Chistoserdova L, Rasche M, Lidstrom M. Novel dephosphotetrahydromethanopterin biosynthesis genes discovered via mutagenesis in Methylobacterium extorquens AM1. J Bacteriol. 2005;187:2508-12 pubmed
    b>Methylobacterium extorquens AM1 was used to explore the genetics of dephosphotetrahydromethanopterin (dH(4)MPT) biosynthesis. Strains with mutations in eight "archaeal-type" genes linked on the chromosome of M...
  31. Verginer M, Siegmund B, Cardinale M, Müller H, Choi Y, Miguez C, et al. Monitoring the plant epiphyte Methylobacterium extorquens DSM 21961 by real-time PCR and its influence on the strawberry flavor. FEMS Microbiol Ecol. 2010;74:136-45 pubmed publisher
    ..Here, we report the selection and characterization of Methylobacterium extorquens DSM 21961, a strain that was able to enhance the furanone content ad planta under greenhouse conditions...
  32. Marx C, Lidstrom M. Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Biotechniques. 2002;33:1062-7 pubmed
    ..We demonstrate this system by generating unmarked deletions of genes in two different bacteria, Methylobacterium extorquens AM1 and Burkholderia fungorum LB400...
  33. Peyraud R, Kiefer P, Christen P, Portais J, Vorholt J. Co-consumption of methanol and succinate by Methylobacterium extorquens AM1. PLoS ONE. 2012;7:e48271 pubmed publisher
    b>Methylobacterium extorquens AM1 is a facultative methylotrophic Alphaproteobacterium and has been subject to intense study under pure methylotrophic as well as pure heterotrophic growth conditions in the past...
  34. Van Dien S, Lidstrom M. Stoichiometric model for evaluating the metabolic capabilities of the facultative methylotroph Methylobacterium extorquens AM1, with application to reconstruction of C(3) and C(4) metabolism. Biotechnol Bioeng. 2002;78:296-312 pubmed
    ..bacterium to evaluate the steady-state growth capabilities of the serine cycle facultative methylotroph Methylobacterium extorquens AM1 during growth on methanol, succinate, and pyruvate...
  35. Kalyuzhnaya M, Lidstrom M. QscR, a LysR-type transcriptional regulator and CbbR homolog, is involved in regulation of the serine cycle genes in Methylobacterium extorquens AM1. J Bacteriol. 2003;185:1229-35 pubmed
    ..regulator that is a homolog of CbbR, has been characterized from the facultative methylotroph Methylobacterium extorquens AM1 and shown to be the major regulator of the serine cycle, the specific C1 assimilation pathway...
  36. Chou H, Marx C. Optimization of gene expression through divergent mutational paths. Cell Rep. 2012;1:133-40 pubmed publisher
    ..Our study revealed diverse molecular changes beneath convergence to a simple phenotype. This complex genotype-phenotype mapping presents a challenge to inferring genetic evolution based solely on phenotypic changes...
  37. Peyraud R, Kiefer P, Christen P, Massou S, Portais J, Vorholt J. Demonstration of the ethylmalonyl-CoA pathway by using 13C metabolomics. Proc Natl Acad Sci U S A. 2009;106:4846-51 pubmed publisher
    ..how glyoxylate is generated during methylotrophic growth in the isocitrate lyase-negative methylotroph Methylobacterium extorquens AM1...
  38. Kiefer P, Portais J, Vorholt J. Quantitative metabolome analysis using liquid chromatography-high-resolution mass spectrometry. Anal Biochem. 2008;382:94-100 pubmed publisher
    ..6 microM. As a case study, intracellular concentrations of central metabolites were determined for Methylobacterium extorquens AM1 during growth on two different carbon sources, methanol and succinate...
  39. Laukel M, Rossignol M, Borderies G, Volker U, Vorholt J. Comparison of the proteome of Methylobacterium extorquens AM1 grown under methylotrophic and nonmethylotrophic conditions. Proteomics. 2004;4:1247-64 pubmed
    b>Methylobacterium extorquens AM1 is a facultative methylotrophic bacterium that is capable of growing in the presence of methanol as the sole carbon and energy source, but is also able to grow on a limited number of C(2), C(3), and C(4) ..
  40. Rasche M, Havemann S, Rosenzvaig M. Characterization of two methanopterin biosynthesis mutants of Methylobacterium extorquens AM1 by use of a tetrahydromethanopterin bioassay. J Bacteriol. 2004;186:1565-70 pubmed
    ..was developed to measure tetrahydromethanopterin (H(4)MPT) levels in wild-type and mutant cells of Methylobacterium extorquens AM1...
  41. Padovani D, Labunska T, Palfey B, Ballou D, Banerjee R. Adenosyltransferase tailors and delivers coenzyme B12. Nat Chem Biol. 2008;4:194-6 pubmed publisher
    ..The strategy of using the final enzyme in an assimilation pathway for tailoring a cofactor and delivering it to a dependent enzyme may be general for cofactor trafficking...
  42. Okubo Y, Skovran E, Guo X, Sivam D, Lidstrom M. Implementation of microarrays for Methylobacterium extorquens AM1. OMICS. 2007;11:325-40 pubmed
    ..To take advantage of this technology, 60-mer oligonucleotide microarrays were designed for Methylobacterium extorquens AM1 to study gene expression changes that occur under differing physiological conditions...
  43. Laukel M, Chistoserdova L, Lidstrom M, Vorholt J. The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: purification and properties. Eur J Biochem. 2003;270:325-33 pubmed
    NAD-dependent formate dehydrogenase (FDH1) was isolated from the alpha-proteobacterium Methylobacterium extorquens AM1 under oxic conditions...
  44. Zhang M, FitzGerald K, Lidstrom M. Identification of an upstream regulatory sequence that mediates the transcription of mox genes in Methylobacterium extorquens AM1. Microbiology. 2005;151:3723-8 pubmed
    ..in the promoter regions for the mxaF, pqqA, mxaW, mxbD and mxcQ genes involved in methanol oxidation in Methylobacterium extorquens AM1, a facultative methylotroph...
  45. Williams P, Coates L, Mohammed F, Gill R, Erskine P, Coker A, et al. The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens. Acta Crystallogr D Biol Crystallogr. 2005;61:75-9 pubmed
    The crystal structure of methanol dehydrogenase (MDH) from Methylobacterium extorquens has been refined without stereochemical restraints at a resolution of 1.2 A...
  46. Muller E, Hourcade E, Louhichi Jelail Y, Hammann P, Vuilleumier S, Bringel F. Functional genomics of dichloromethane utilization in Methylobacterium extorquens DM4. Environ Microbiol. 2011;13:2518-35 pubmed publisher
    ..In Methylobacterium extorquens DM4 of known genome sequence, dcmA lies on a 126 kb dcm genomic island not found so far in other DCM-..
  47. Toyama H, Inagaki H, Matsushita K, Anthony C, Adachi O. The role of the MxaD protein in the respiratory chain of Methylobacterium extorquens during growth on methanol. Biochim Biophys Acta. 2003;1647:372-5 pubmed
  48. Zhang M, Lidstrom M. Promoters and transcripts for genes involved in methanol oxidation in Methylobacterium extorquens AM1. Microbiology. 2003;149:1033-40 pubmed
    ..methanol oxidation to formaldehyde by the methanol dehydrogenase system in the facultative methylotroph Methylobacterium extorquens AM1 organized in five gene clusters...
  49. Van Dien S, Strovas T, Lidstrom M. Quantification of central metabolic fluxes in the facultative methylotroph methylobacterium extorquens AM1 using 13C-label tracing and mass spectrometry. Biotechnol Bioeng. 2003;84:45-55 pubmed
    The metabolic fluxes of central carbon metabolism were measured in chemostat-grown cultures of Methylobacterium extorquens AM1 with methanol as the sole organic carbon and energy source and growth-limiting substrate...
  50. Marx C, Laukel M, Vorholt J, Lidstrom M. Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth. J Bacteriol. 2003;185:7169-75 pubmed
    The serine cycle methylotroph Methylobacterium extorquens AM1 contains two pterin-dependent pathways for C(1) transfers, the tetrahydrofolate (H(4)F) pathway and the tetrahydromethanopterin (H(4)MPT) pathway, and both are required for ..
  51. Caccamo M, Malone C, Rasche M. Biochemical characterization of a dihydromethanopterin reductase involved in tetrahydromethanopterin biosynthesis in Methylobacterium extorquens AM1. J Bacteriol. 2004;186:2068-73 pubmed
    During growth on one-carbon (C1) compounds, the aerobic alpha-proteobacterium Methylobacterium extorquens AM1 synthesizes the tetrahydromethanopterin (H4MPT) derivative dephospho-H4MPT as a C1 carrier in addition to tetrahydrofolate...
  52. Kalyuzhnaya M, Lidstrom M. QscR-mediated transcriptional activation of serine cycle genes in Methylobacterium extorquens AM1. J Bacteriol. 2005;187:7511-7 pubmed
    QscR, a LysR-type regulator, is the major regulator of assimilatory C1 metabolism in Methylobacterium extorquens AM1...
  53. Scott J, Rasche M. Purification, overproduction, and partial characterization of beta-RFAP synthase, a key enzyme in the methanopterin biosynthesis pathway. J Bacteriol. 2002;184:4442-8 pubmed
    ..homologs in additional archaea and in a gene cluster required for C1 metabolism by the bacterium Methylobacterium extorquens. The identification of a gene encoding a potential beta-RFAP synthase in M...
  54. Chistoserdova L, Laukel M, Portais J, Vorholt J, Lidstrom M. Multiple formate dehydrogenase enzymes in the facultative methylotroph Methylobacterium extorquens AM1 are dispensable for growth on methanol. J Bacteriol. 2004;186:22-8 pubmed
    ..was used to identify three different formate dehydrogenase systems in the facultative methylotroph Methylobacterium extorquens AM1 whose expression is affected by either molybdenum or tungsten...
  55. Chistoserdova L, Chen S, Lapidus A, Lidstrom M. Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view. J Bacteriol. 2003;185:2980-7 pubmed
  56. Choi Y, Bourque D, Morel L, Groleau D, Miguez C. Multicopy integration and expression of heterologous genes in Methylobacterium extorquens ATCC 55366. Appl Environ Microbiol. 2006;72:753-9 pubmed
    High-level expression of chromosomally integrated genes in Methylobacterium extorquens ATCC 55366 was achieved under the control of the strong M. extorquens AM1 methanol dehydrogenase promoter (PmxaF) using the mini-Tn7 transposon system...
  57. Delaney N, Kaczmarek M, Ward L, Swanson P, Lee M, Marx C. Development of an optimized medium, strain and high-throughput culturing methods for Methylobacterium extorquens. PLoS ONE. 2013;8:e62957 pubmed publisher
    b>Methylobacterium extorquens strains are the best-studied methylotrophic model system, and their metabolism of single carbon compounds has been studied for over 50 years. Here we develop a new system for high-throughput batch culture of M...
  58. Bosch G, Skovran E, Xia Q, Wang T, Taub F, Miller J, et al. Comprehensive proteomics of Methylobacterium extorquens AM1 metabolism under single carbon and nonmethylotrophic conditions. Proteomics. 2008;8:3494-505 pubmed publisher
    In order to validate a gel free quantitative proteomics assay for the model methylotrophic bacterium Methylobacterium extorquens AM1, we examined the M...
  59. Pomper B, Vorholt J. Characterization of the formyltransferase from Methylobacterium extorquens AM1. Eur J Biochem. 2001;268:4769-75 pubmed
    b>Methylobacterium extorquens AM1 possesses a formaldehyde-oxidation pathway that involves enzymes with high sequence identity with enzymes from methanogenic and sulfate-reducing archaea...
  60. Skovran E, Crowther G, Guo X, Yang S, Lidstrom M. A systems biology approach uncovers cellular strategies used by Methylobacterium extorquens AM1 during the switch from multi- to single-carbon growth. PLoS ONE. 2010;5:e14091 pubmed publisher
    ..experience environmental change, how does their metabolic network reset and adapt to the new condition? Methylobacterium extorquens is a bacterium capable of growth on both multi- and single-carbon compounds...
  61. Gourion B, Francez Charlot A, Vorholt J. PhyR is involved in the general stress response of Methylobacterium extorquens AM1. J Bacteriol. 2008;190:1027-35 pubmed
    ..PhyR was first described in Methylobacterium extorquens AM1, in which it has been shown to be essential for plant colonization, probably due to its suggested ..
  62. Korotkova N, Lidstrom M. Connection between poly-beta-hydroxybutyrate biosynthesis and growth on C(1) and C(2) compounds in the methylotroph Methylobacterium extorquens AM1. J Bacteriol. 2001;183:1038-46 pubmed
    ..from genomic sequence data and have been characterized in the serine cycle facultative methylotroph Methylobacterium extorquens AM1...