leptospira interrogans

Summary

Summary: A genus of question mark-shaped bacteria spirochetes which is found in fresh water that is contaminated by animal urine. It causes LEPTOSPIROSIS.

Top Publications

  1. Ren S, Fu G, Jiang X, Zeng R, Miao Y, Xu H, et al. Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing. Nature. 2003;422:888-93 pubmed
    ..Here we report the complete genomic sequence of a representative virulent serovar type strain (Lai) of Leptospira interrogans serogroup Icterohaemorrhagiae consisting of a 4...
  2. Pinne M, Choy H, Haake D. The OmpL37 surface-exposed protein is expressed by pathogenic Leptospira during infection and binds skin and vascular elastin. PLoS Negl Trop Dis. 2010;4:e815 pubmed publisher
    ..The binding to human skin elastin by both recombinant OmpL37 and live Leptospira interrogans is specifically enhanced by rabbit antiserum for OmpL37, suggesting the involvement of OmpL37 in ..
  3. Bulach D, Zuerner R, Wilson P, Seemann T, McGrath A, Cullen P, et al. Genome reduction in Leptospira borgpetersenii reflects limited transmission potential. Proc Natl Acad Sci U S A. 2006;103:14560-5 pubmed
    ..Most infections are caused by either Leptospira borgpetersenii or Leptospira interrogans, bacteria that vary in their distribution in nature and rely on different modes of transmission...
  4. Li S, Ojcius D, Liao S, Li L, Xue F, Dong H, et al. Replication or death: distinct fates of pathogenic Leptospira strain Lai within macrophages of human or mouse origin. Innate Immun. 2010;16:80-92 pubmed publisher
    ..The reasons for this diversity of infection remain unclear. Here, we demonstrated that Leptospira interrogans serovar Lai strain Lai had a similar ability to adhere to and enter primary and immortal (THP-1 and J774A...
  5. Vieira M, de Morais Z, Gonçales A, Romero E, Vasconcellos S, Nascimento A. Lsa63, a newly identified surface protein of Leptospira interrogans binds laminin and collagen IV. J Infect. 2010;60:52-64 pubmed publisher
    b>Leptospira interrogans is the etiological agent of leptospirosis, a zoonotic disease that affects populations worldwide...
  6. Cullen P, Cordwell S, Bulach D, Haake D, Adler B. Global analysis of outer membrane proteins from Leptospira interrogans serovar Lai. Infect Immun. 2002;70:2311-8 pubmed
    ..Outer membrane fractions were isolated from Leptospira interrogans serovar Lai grown at 20, 30, and 37 degrees C with or without 10% fetal calf serum and, finally, in iron-..
  7. Thaipadungpanit J, Wuthiekanun V, Chierakul W, Smythe L, Petkanchanapong W, Limpaiboon R, et al. A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand. PLoS Negl Trop Dis. 2007;1:e56 pubmed
    ..One of these (ST34) accounted for 77 (76%) of isolates. ST34 was Leptospira interrogans, serovar Autumnalis...
  8. Lessa Aquino C, Borges Rodrigues C, Pablo J, Sasaki R, Jasinskas A, Liang L, et al. Identification of seroreactive proteins of Leptospira interrogans serovar copenhageni using a high-density protein microarray approach. PLoS Negl Trop Dis. 2013;7:e2499 pubmed publisher
    ..be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response from 274 individuals, including 80 acute-phase, 80 convalescent-..
  9. Branger C, Chatrenet B, Gauvrit A, Aviat F, Aubert A, Bach J, et al. Protection against Leptospira interrogans sensu lato challenge by DNA immunization with the gene encoding hemolysin-associated protein 1. Infect Immun. 2005;73:4062-9 pubmed
    ..This finding should facilitate the design and development of a new generation of vaccines against bacteria, particularly Leptospira interrogans sensu lato.

More Information

Publications101 found, 100 shown here

  1. Gaudie C, Featherstone C, Phillips W, McNaught R, Rhodes P, Errington J, et al. Human Leptospira interrogans serogroup icterohaemorrhagiae infection (Weil's disease) acquired from pet rats. Vet Rec. 2008;163:599-601 pubmed
  2. Que Gewirth N, Ribeiro A, Kalb S, Cotter R, Bulach D, Adler B, et al. A methylated phosphate group and four amide-linked acyl chains in leptospira interrogans lipid A. The membrane anchor of an unusual lipopolysaccharide that activates TLR2. J Biol Chem. 2004;279:25420-9 pubmed
    b>Leptospira interrogans differs from other spirochetes in that it contains homologs of all the Escherichia coli lpx genes required for the biosynthesis of the lipid A anchor of lipopolysaccharide (LPS). LPS from L...
  3. Lin Y, Lee D, McDonough S, Nicholson L, Sharma Y, Chang Y. Repeated domains of leptospira immunoglobulin-like proteins interact with elastin and tropoelastin. J Biol Chem. 2009;284:19380-91 pubmed publisher
    ..6-fold less than that of wild type LigBCon4. In summary, we show that Lig proteins of Leptospira spp. interact with elastin and HTE, and we conclude this interaction may contribute to Leptospira adhesion to host tissues during infection. ..
  4. Picardeau M, Ren S, Saint Girons I. Killing effect and antitoxic activity of the Leptospira interrogans toxin-antitoxin system in Escherichia coli. J Bacteriol. 2001;183:6494-7 pubmed publisher
    ..This locus has been found in the pathogenic spirochete Leptospira interrogans and exhibits homologies with the pem/chp loci. The L...
  5. Nascimento A, Verjovski Almeida S, Van Sluys M, Monteiro Vitorello C, Camargo L, Digiampietri L, et al. Genome features of Leptospira interrogans serovar Copenhageni. Braz J Med Biol Res. 2004;37:459-77 pubmed
    We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete...
  6. Verma A, Artiushin S, Matsunaga J, Haake D, Timoney J. LruA and LruB, novel lipoproteins of pathogenic Leptospira interrogans associated with equine recurrent uveitis. Infect Immun. 2005;73:7259-66 pubmed
    ..in the eyes of horses with lesions of chronic uveitis were identified by screening a phage library of Leptospira interrogans DNA fragments with eye fluids from uveitic horses...
  7. Barbosa A, Abreu P, Neves F, Atzingen M, Watanabe M, Vieira M, et al. A newly identified leptospiral adhesin mediates attachment to laminin. Infect Immun. 2006;74:6356-64 pubmed
    ..Six predicted coding sequences selected from the Leptospira interrogans serovar Copenhageni genome were cloned, and proteins were expressed, purified by metal affinity ..
  8. Matsunaga J, Medeiros M, Sanchez Y, Werneid K, Ko A. Osmotic regulation of expression of two extracellular matrix-binding proteins and a haemolysin of Leptospira interrogans: differential effects on LigA and Sph2 extracellular release. Microbiology. 2007;153:3390-8 pubmed
    The life cycle of the pathogen Leptospira interrogans involves stages outside and inside the host. Entry of L...
  9. Lo M, Bulach D, Powell D, Haake D, Matsunaga J, Paustian M, et al. Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays. Infect Immun. 2006;74:5848-59 pubmed
    ..from infected animals or contaminated water or soil. The availability of genome sequences for Leptospira interrogans, serovars Lai and Copenhageni, has opened up opportunities to examine global transcription profiles using ..
  10. Choy H, Kelley M, Chen T, Møller A, Matsunaga J, Haake D. Physiological osmotic induction of Leptospira interrogans adhesion: LigA and LigB bind extracellular matrix proteins and fibrinogen. Infect Immun. 2007;75:2441-50 pubmed
    ..The characterization of the Lig adhesin function should aid the design of Lig-based vaccines and serodiagnostic tests. ..
  11. Vernel Pauillac F, Merien F. Proinflammatory and immunomodulatory cytokine mRNA time course profiles in hamsters infected with a virulent variant of Leptospira interrogans. Infect Immun. 2006;74:4172-9 pubmed
    ..of cytokine mRNA in the peripheral blood mononuclear cells of both control (uninfected) hamsters and Leptospira interrogans-inoculated hamsters from 1 to 24 h and then 1 to 4 days postinfection...
  12. Claassen M, Aebersold R, Buhmann J. Proteome coverage prediction for integrated proteomics datasets. J Comput Biol. 2011;18:283-93 pubmed publisher
    ..interrogans dataset. We discuss the implications of our results for determining rational stop criteria and their influence on the design of efficient and reliable proteomics studies. ..
  13. Kariv R, Klempfner R, Barnea A, Sidi Y, Schwartz E. The changing epidemiology of leptospirosis in Israel. Emerg Infect Dis. 2001;7:990-2 pubmed
    ..Serogroups that occurred mainly in rural areas accounted previously for 79% but had declined to 32%. ..
  14. Meri T, Murgia R, Stefanel P, Meri S, Cinco M. Regulation of complement activation at the C3-level by serum resistant leptospires. Microb Pathog. 2005;39:139-47 pubmed
    ..Thus, binding of this major alternative complement pathway inhibitor is related to serum resistance in Leptospira spirochetes...
  15. Domingos R, Vieira M, Romero E, Gonçales A, de Morais Z, Vasconcellos S, et al. Features of two proteins of Leptospira interrogans with potential role in host-pathogen interactions. BMC Microbiol. 2012;12:50 pubmed publisher
    ..To our knowledge, Lsa33 is the first leptospiral protein described to date with the capability of binding laminin, PLG and C4bp in vitro. ..
  16. Nally J, Artiushin S, Timoney J. Molecular characterization of thermoinduced immunogenic proteins Q1p42 and Hsp15 of Leptospira interrogans. Infect Immun. 2001;69:7616-24 pubmed
    b>Leptospira interrogans is a mammalian pathogen which must adapt to a range of new environmental conditions including temperature change when it infects new hosts...
  17. Murray G, Ellis K, Lo M, Adler B. Leptospira interrogans requires a functional heme oxygenase to scavenge iron from hemoglobin. Microbes Infect. 2008;10:791-7 pubmed publisher
    The transposon TnSC189 was used to construct a mutant in the putative heme oxygenase gene hemO (LB186) of Leptospira interrogans. Unlike its parent strain, the mutant grew poorly in medium in which hemoglobin was the sole iron source...
  18. Oliveira T, Longhi M, Gonçales A, de Morais Z, Vasconcellos S, Nascimento A. LipL53, a temperature regulated protein from Leptospira interrogans that binds to extracellular matrix molecules. Microbes Infect. 2010;12:207-17 pubmed publisher
    ..In this study, we characterize the protein LipL53 from Leptospira interrogans, previously shown to react with serum sample of individual diagnosed with leptospirosis and to be up-..
  19. Rossetti C, Liem M, Samartino L, Hartskeerl R. Buenos Aires, a new Leptospira serovar of serogroup Djasiman, isolated from an aborted dog fetus in Argentina. Vet Microbiol. 2005;107:241-8 pubmed
    ..However, the isolate could not be identified as any of the known serovars within this serogroup. These results allow us to conclude that the strain Baires represents a new serovar (Buenos Aires) of Djasiman serogroup. ..
  20. Murray G, Srikram A, Henry R, Puapairoj A, Sermswan R, Adler B. Leptospira interrogans requires heme oxygenase for disease pathogenesis. Microbes Infect. 2009;11:311-4 pubmed publisher
    We recently characterised the Leptospira interrogans heme oxygenase (hemO) gene and showed that HemO was required for growth with hemoglobin as the sole iron source. Here we investigated the role of HemO in pathogenesis...
  21. Schmidt A, Beck M, Malmstrom J, Lam H, Claassen M, Campbell D, et al. Absolute quantification of microbial proteomes at different states by directed mass spectrometry. Mol Syst Biol. 2011;7:510 pubmed publisher
    ..to detect and quantify sets of pre-determined peptides in tryptic digests of cells of the human pathogen Leptospira interrogans at 25 different states. We show that in a single LC-MS/MS experiment around 5000 peptides, covering 1680 L...
  22. Athanazio D, Silva E, Santos C, Rocha G, Vannier Santos M, McBride A, et al. Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans. Acta Trop. 2008;105:176-80 pubmed
    ..In this study, Wistar rats were infected intraperitoneally with virulent Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130...
  23. Thompson J, de Miranda Henriques Leite R, Gonçalves V, Leite R, Bandeira D, Herrmann G, et al. Spatial hierarchical variances and age covariances for seroprevalence to Leptospira interrogans serovar hardjo, BoHV-1 and BVDV for cattle in the State of Paraíba, Brazil. Prev Vet Med. 2006;76:290-301 pubmed
    We estimated spatial hierarchal variances and age-group covariances for seroprevalence to Leptospira interrogans serovar hardjo (LeptoH), bovine viral-diarrhea virus (BVDV) and bovine herpesvirus type 1 (BoHV-1) among 2343 cattle from 72 ..
  24. Viratyosin W, Ingsriswang S, Pacharawongsakda E, Palittapongarnpim P. Genome-wide subcellular localization of putative outer membrane and extracellular proteins in Leptospira interrogans serovar Lai genome using bioinformatics approaches. BMC Genomics. 2008;9:181 pubmed publisher
    ..efforts have been made to identify outer membrane (OM) and extracellular (EX) proteins produced by Leptospira interrogans, which may be used as novel targets for the development of infection markers and leptospirosis vaccines...
  25. Vijayachari P, Sehgal S, Goris M, Terpstra W, Hartskeerl R. Leptospira interrogans serovar Valbuzzi: a cause of severe pulmonary haemorrhages in the Andaman Islands. J Med Microbiol. 2003;52:913-8 pubmed
    ..The isolates showed 97.5-100 % sequence similarity to reference strains belonging to Leptospira interrogans, indicating that the isolates belong to L. interrogans...
  26. Qin J, Sheng Y, Zhang Z, Shi Y, He P, Hu B, et al. Genome-wide transcriptional analysis of temperature shift in L. interrogans serovar lai strain 56601. BMC Microbiol. 2006;6:51 pubmed
    b>Leptospira interrogans is an important mammalian pathogen. Transmission from an environmental source requires adaptation to a range of new environmental conditions in the organs and tissues of the infected host...
  27. Chirathaworn C, Patarakul K, Saksit V, Poovorawan Y. Binding of Leptospira to extracellular matrix proteins. J Med Assoc Thai. 2007;90:2136-42 pubmed
    ..However, this capability may not be the only mechanism that makes leptospires virulent since non-pathogenic leptospire can bind the ECM as well. ..
  28. Monahan A, Callanan J, Nally J. Proteomic analysis of Leptospira interrogans shed in urine of chronically infected hosts. Infect Immun. 2008;76:4952-8 pubmed publisher
    ..Results confirm that differential protein expression by Leptospira during chronic infection facilitates its persistence in the presence of a specific host antibody response. ..
  29. Habarta A, Abreu P, Olivera N, Hauk P, Cédola M, Ferrer M, et al. Increased immunogenicity to LipL32 of Leptospira interrogans when expressed as a fusion protein with the cholera toxin B subunit. Curr Microbiol. 2011;62:526-31 pubmed publisher
    ..The development of a recombinant leptospira vaccine remains a challenge. In this study, we cloned the Leptospira interrogans open reading frame (ORF) coding the external membrane protein LipL32, an immunodominant antigen found in ..
  30. Lucas D, Cullen P, Lo M, Srikram A, Sermswan R, Adler B. Recombinant LipL32 and LigA from Leptospira are unable to stimulate protective immunity against leptospirosis in the hamster model. Vaccine. 2011;29:3413-8 pubmed publisher
    ..repeat domains of LigA (LigANI) and a LipL32-LigANI fusion protein in hamsters against infection with Leptospira interrogans serovar Manilae...
  31. Benacer D, Mohd Zain S, Amran F, Galloway R, Thong K. Isolation and molecular characterization of Leptospira interrogans and Leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia. Am J Trop Med Hyg. 2013;88:704-9 pubmed publisher
    ..Despite the low infection rate reported, the high pathogenicity of these serovars raises concern of public health risks caused by rodent transmission of leptospirosis. ..
  32. Diament D, Brunialti M, Romero E, Kallas E, Salomao R. Peripheral blood mononuclear cell activation induced by Leptospira interrogans glycolipoprotein. Infect Immun. 2002;70:1677-83 pubmed
    b>Leptospira interrogans glycolipoprotein (GLP) has been implicated in pathological and functional derangement seen in leptospirosis...
  33. Nally J, Whitelegge J, Aguilera R, Pereira M, Blanco D, Lovett M. Purification and proteomic analysis of outer membrane vesicles from a clinical isolate of Leptospira interrogans serovar Copenhageni. Proteomics. 2005;5:144-52 pubmed
  34. Gomez R, Vieira M, Schattner M, Malaver E, Watanabe M, Barbosa A, et al. Putative outer membrane proteins of Leptospira interrogans stimulate human umbilical vein endothelial cells (HUVECS) and express during infection. Microb Pathog. 2008;45:315-22 pubmed publisher
    ..that lead to the upregulation of CAMs was reported for the pathogenic spirochaetes, including rLIC10365 of Leptospira interrogans. In this study, we report the cloning of LIC10507, LIC10508, LIC10509 genes of L...
  35. Jin D, Ojcius D, Sun D, Dong H, Luo Y, Mao Y, et al. Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-dependent pathways. Infect Immun. 2009;77:799-809 pubmed publisher
    ..It has been reported that Leptospira interrogans, the causal agent of leptospirosis, induces apoptosis in macrophages and hepatocytes...
  36. Jimenez Coello M, Ortega Pacheco A, Guzman Marin E, Guiris Andrade D, Martinez Figueroa L, Acosta Viana K. Stray dogs as reservoirs of the zoonotic agents Leptospira interrogans, Trypanosoma cruzi, and Aspergillus spp. in an urban area of Chiapas in southern Mexico. Vector Borne Zoonotic Dis. 2010;10:135-41 pubmed publisher
    This investigation determined the presence and prevalence of the zoonotic agents Leptospira interrogans, Trypanosoma cruzi, and Aspergillus spp. in the stray dog population (a total of 224 stray dogs) in an urban area of Southern Mexico...
  37. Cao X, Dai J, Xu H, Nie S, Chang X, Hu B, et al. High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans. Cell Res. 2010;20:197-210 pubmed publisher
    ..computational prediction and high-accuracy tandem mass spectra, we revised the genome annotation of Leptospira interrogans serovar Lai, a free-living pathogenic spirochete responsible for leptospirosis, providing substantial ..
  38. Fraga T, Chura Chambi R, Gonçales A, Morais Z, Vasconcellos S, Morganti L, et al. Refolding of the recombinant protein OmpA70 from Leptospira interrogans from inclusion bodies using high hydrostatic pressure and partial characterization of its immunological properties. J Biotechnol. 2010;148:156-62 pubmed publisher
    ..The present work studied OmpA70, a putative outer membrane protein from Leptospira interrogans serovar Copenhageni that combines structural features of Loa22, the first genetically defined virulence ..
  39. Xue F, Dong H, Wu J, Wu Z, Hu W, Sun A, et al. Transcriptional responses of Leptospira interrogans to host innate immunity: significant changes in metabolism, oxygen tolerance, and outer membrane. PLoS Negl Trop Dis. 2010;4:e857 pubmed publisher
    b>Leptospira interrogans is the major causative agent of leptospirosis. Phagocytosis plays important roles in the innate immune responses to L. interrogans infection, and L. interrogans can evade the killing of phagocytes...
  40. Wu B, Bao L, Sun Z, Li D, Zhang Y. [The OmpA-like protein Loa22 from Leptospira interrogans serovar lai induces apoptosis in A549 via Ca2+ signal pathway]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2011;42:298-302 pubmed
    ..To study the effect of Loa22 mature peptide on the apoptosis of A549 to explore the mechanism of pulmonary impairment in severe forms of leptospirosis...
  41. Lee S, Kim S, Park S, Kim M. Cytotoxic activities of Leptospira interrogans hemolysin SphH as a pore-forming protein on mammalian cells. Infect Immun. 2002;70:315-22 pubmed
    ..Previously, we cloned a hemolysin SphH of Leptospira interrogans serovar lai, a homologue of L. borgpetersenii sphingomyelinase (SphA), from a genomic library (S. H...
  42. Nally J, Chow E, Fishbein M, Blanco D, Lovett M. Changes in lipopolysaccharide O antigen distinguish acute versus chronic Leptospira interrogans infections. Infect Immun. 2005;73:3251-60 pubmed
    ..The hypothesis that the Oag content is related to whether lethal infection or chronic renal tubular colonization occurs remains to be tested...
  43. Nahori M, Fournié Amazouz E, Que Gewirth N, Balloy V, Chignard M, Raetz C, et al. Differential TLR recognition of leptospiral lipid A and lipopolysaccharide in murine and human cells. J Immunol. 2005;175:6022-31 pubmed
    b>Leptospira interrogans is a spirochete that is responsible for leptospirosis, a zoonotic disease. This bacterium possesses an unusual LPS that has been shown to use TLR2 instead of TLR4 for signaling in human cells...
  44. Louvel H, Bommezzadri S, Zidane N, Boursaux Eude C, Creno S, Magnier A, et al. Comparative and functional genomic analyses of iron transport and regulation in Leptospira spp. J Bacteriol. 2006;188:7893-904 pubmed publisher
    ..biflexa. Comparative genomics, in combination with gene inactivation, gives us significant functional information on iron homeostasis in Leptospira spp...
  45. Matsunaga J, Lo M, Bulach D, Zuerner R, Adler B, Haake D. Response of Leptospira interrogans to physiologic osmolarity: relevance in signaling the environment-to-host transition. Infect Immun. 2007;75:2864-74 pubmed
    ..Invasion of host tissues by Leptospira interrogans involves a transition from a low osmolar environment outside the host to a higher physiologic osmolar ..
  46. Bezerra da Silva J, Carvalho E, Hartskeerl R, Ho P. Evaluation of the use of selective PCR amplification of LPS biosynthesis genes for molecular typing of leptospira at the serovar level. Curr Microbiol. 2011;62:518-24 pubmed publisher
    ..of polymorphic regions from the LPS biosynthesis loci (rfb) as a potential tool for serovar typing of Leptospira interrogans species...
  47. Lo M, Murray G, Khoo C, Haake D, Zuerner R, Adler B. Transcriptional response of Leptospira interrogans to iron limitation and characterization of a PerR homolog. Infect Immun. 2010;78:4850-9 pubmed publisher
    ..Our results provide insights into the role of iron in leptospiral metabolism and regulation of the oxidative stress response, including genes likely to be important for virulence...
  48. Bandeira M, Santos C, de Azevedo E, Soares L, Macedo J, Marchi S, et al. Attenuated nephritis in inducible nitric oxide synthase knockout C57BL/6 mice and pulmonary hemorrhage in CB17 SCID and recombination activating gene 1 knockout C57BL/6 mice infected with Leptospira interrogans. Infect Immun. 2011;79:2936-40 pubmed publisher
    ..We studied the outcome of infection by the virulent Leptospira interrogans serovar Copenhageni strain Cop...
  49. Palaniappan R, Chang Y, Jusuf S, Artiushin S, Timoney J, McDonough S, et al. Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans. Infect Immun. 2002;70:5924-30 pubmed
    A clone expressing a novel immunoreactive leptospiral immunoglobulin-like protein A of 130 kDa (LigA) from Leptospira interrogans serovar pomona type kennewicki was isolated by screening a genomic DNA library with serum from a mare that ..
  50. Acevedo Whitehouse K, de la Cueva H, Gulland F, Aurioles Gamboa D, Arellano Carbajal F, Suárez Güemes F. Evidence of Leptospira interrogans infection in California sea lion pups from the Gulf of California. J Wildl Dis. 2003;39:145-51 pubmed
    ..Antibody prevalence to 13 serovars of Leptospira interrogans was determined by microagglutination tests (MAT); presence of pathogenic leptospires was detected by ..
  51. Zhang Y, Geng Y, Bi B, He J, Wu C, Guo X, et al. Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohae-morrhagiae serovar Lai. Acta Pharmacol Sin. 2005;26:453-61 pubmed
    To identify and classify all potential hemolysin candidates of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai.
  52. Roy S, Biswas D, Vijayachari P, Sugunan A, Sehgal S. A clone of Leptospira interrogans sensu stricto is the major cause of leptospirosis in the archipelago of Andaman and Nicobar Islands, India. Lett Appl Microbiol. 2005;41:179-85 pubmed
    ..Several isolates have been recovered over the years from different locations. The present study was undertaken to understand the clonal relationship between all these pathogenic leptospires recovered from these islands...
  53. da Silva J, Ramos T, De Franco M, Paiva D, Ho P, Martins E, et al. Chemokines expression during Leptospira interrogans serovar Copenhageni infection in resistant BALB/c and susceptible C3H/HeJ mice. Microb Pathog. 2009;47:87-93 pubmed publisher
    ..A virulent strain of Leptospira interrogans serovar Copenhageni was used in this study...
  54. Patarakul K, Lo M, Adler B. Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum. BMC Microbiol. 2010;10:31 pubmed publisher
    ..Previous DNA microarray studies have identified differentially expressed genes required for adaptation to temperature and osmolarity conditions inside the host compared to those of the environment...
  55. Zhong Y, Chang X, Cao X, Zhang Y, Zheng H, Zhu Y, et al. Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601. Cell Res. 2011;21:1210-29 pubmed publisher
    The virulence-attenuated Leptospira interrogans serovar Lai strain IPAV was derived by prolonged laboratory passage from a highly virulent ancestral strain isolated in China...
  56. Wang H, Wu Y, Ojcius D, Yang X, Zhang C, Ding S, et al. Leptospiral hemolysins induce proinflammatory cytokines through Toll-like receptor 2-and 4-mediated JNK and NF-?B signaling pathways. PLoS ONE. 2012;7:e42266 pubmed publisher
  57. Scialfa E, Brihuega B, Venzano A, Morris W, Bolpe J, Schettino M. First isolation of Leptospira interrogans from Lycalopex griseus (South American gray fox) in Argentina shows new MLVA genotype. J Wildl Dis. 2013;49:168-72 pubmed publisher
    ..microscopic agglutination test, and five (two gray foxes, two armadillos, and one skunk) were positive for Leptospira interrogans serovars Canicola and Icterohaemorrhagiae, L. borgpetersenii serovar Castellonis, and L...
  58. Li Y, Ren S, Gong W. Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans. Acta Crystallogr D Biol Crystallogr. 2002;58:846-8 pubmed
    A new peptide deformylase (PDF; EC 3.5.1.27) gene from Leptospira interrogans was identified and cloned into expression plasmid pET22b(+) and was highly expressed in Escherichia coli BL21(DE3)...
  59. Romero E, Yasuda P. Molecular characterization of Leptospira sp. strains isolated from human subjects in São Paulo, Brazil using a polymerase chain reaction-based assay: a public health tool. Mem Inst Oswaldo Cruz. 2006;101:373-8 pubmed
    ..5%) and one to serogroup Sejroe (2.5%). These results demonstrate that the PCR method described in this study has utility for rapid typing of Leptospira sp. at the serogroup level and can be used in epidemiological survey...
  60. Laurichesse H, Gourdon F, Smits H, Abdoe T, Estavoyer J, Rebika H, et al. Safety and immunogenicity of subcutaneous or intramuscular administration of a monovalent inactivated vaccine against Leptospira interrogans serogroup Icterohaemorrhagiae in healthy volunteers. Clin Microbiol Infect. 2007;13:395-403 pubmed
    The safety and immunogenicity of a monovalent inactivated vaccine against Leptospira interrogans serogroup Icterohaemorrhagiae was evaluated in 84 volunteers according to the route of administration, i.e...
  61. Seixas F, da Silva E, Hartwig D, Cerqueira G, Amaral M, Fagundes M, et al. Recombinant Mycobacterium bovis BCG expressing the LipL32 antigen of Leptospira interrogans protects hamsters from challenge. Vaccine. 2007;26:88-95 pubmed
    ..The gene coding for the Leptospira interrogans external membrane protein LipL32, a highly immunogenic antigen found in all pathogenic leptospira, was ..
  62. Koizumi N, Muto M, Tanikawa T, Mizutani H, Sohmura Y, Hayashi E, et al. Human leptospirosis cases and the prevalence of rats harbouring Leptospira interrogans in urban areas of Tokyo, Japan. J Med Microbiol. 2009;58:1227-30 pubmed publisher
    ..Most of these rat isolates were Leptospira interrogans serovar Copenhageni/Icterohaemorrhagiae...
  63. Longhi M, Oliveira T, Romero E, Gonçales A, de Morais Z, Vasconcellos S, et al. A newly identified protein of Leptospira interrogans mediates binding to laminin. J Med Microbiol. 2009;58:1275-82 pubmed publisher
    ..Taken together, these data indicate that this newly identified membrane protein is expressed during natural infection and may play a role in mediating adhesion of L. interrogans to its host...
  64. Lin Y, Greenwood A, Nicholson L, Sharma Y, McDonough S, Chang Y. Fibronectin binds to and induces conformational change in a disordered region of leptospiral immunoglobulin-like protein B. J Biol Chem. 2009;284:23547-57 pubmed publisher
    b>Leptospira interrogans is a pathogenic spirochete that causes disease in both humans and animals...
  65. Zhang Y, Bao L, Zhu H, Huang B, Zhang H. OmpA-like protein Loa22 from Leptospira interrogans serovar Lai is cytotoxic to cultured rat renal cells and promotes inflammatory responses. Acta Biochim Biophys Sin (Shanghai). 2010;42:70-9 pubmed
    ..Outer membrane protein A-like protein Loa22 is a lipoprotein from Leptospira interrogans and has been suggested to be a corresponding virulence factor...
  66. Coutinho M, Choy H, Kelley M, Matsunaga J, Babbitt J, Lewis M, et al. A LigA three-domain region protects hamsters from lethal infection by Leptospira interrogans. PLoS Negl Trop Dis. 2011;5:e1422 pubmed publisher
    ..subunit vaccine that has been demonstrated to protect against lethal challenge by a clinical isolate of Leptospira interrogans in the hamster model of leptospirosis...
  67. Souza N, Vieira M, Alves I, de Morais Z, Vasconcellos S, Nascimento A. Lsa30, a novel adhesin of Leptospira interrogans binds human plasminogen and the complement regulator C4bp. Microb Pathog. 2012;53:125-34 pubmed publisher
    ..Thus, Lsa30 is a novel versatile protein that may play a role in mediating adhesion and may help pathogenic Leptospira to overcome tissue barriers and to escape the immune system...
  68. Sunbul M, Esen S, Leblebicioglu H, Hokelek M, Pekbay A, Eroglu C. Rattus norvegicus acting as reservoir of leptospira interrogans in the Middle Black Sea region of Turkey, as evidenced by PCR and presence of serum antibodies to Leptospira strain. Scand J Infect Dis. 2001;33:896-8 pubmed
    Leptospirosis, a common form of zoonosis, especially in rainy countries, is caused by Leptospira interrogans. In our region of Turkey this type of disease has often been encountered in connection with rice harvesting and we therefore ..
  69. Barocchi M, Ko A, Reis M, McDonald K, Riley L. Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen. Infect Immun. 2002;70:6926-32 pubmed
    ..In this study, we used a low-passage-number isolate of Leptospira interrogans to elucidate the invasive potential of this spirochete...
  70. Marangoni A, Aldini R, Sambri V, Giacani L, Di Leo K, Cevenini R. Production of tumor necrosis factor alpha by Treponema pallidum, Borrelia burgdorferi s.l., and Leptospira interrogans in isolated rat Kupffer cells. FEMS Immunol Med Microbiol. 2004;40:187-91 pubmed
    Stimulation of isolated rat Kupffer cells by viable Leptospira interrogans, Treponema pallidum and Borrelia garinii elicited cellular responses resulting in the release of different tumor necrosis factor alpha (TNF-alpha) levels, ..
  71. Zhou Z, Song X, Li Y, Gong W. Unique structural characteristics of peptide deformylase from pathogenic bacterium Leptospira interrogans. J Mol Biol. 2004;339:207-15 pubmed publisher
    ..Here, we present the crystal structure of Leptospira interrogans PDF (LiPDF) at 2.2A resolution...
  72. Trueba G, Zapata S, Madrid K, Cullen P, Haake D. Cell aggregation: a mechanism of pathogenic Leptospira to survive in fresh water. Int Microbiol. 2004;7:35-40 pubmed
    ..In the present study, the survival mechanisms of Leptospira interrogans serovar Canicola in aqueous conditions and lack of nutrients were investigated...
  73. Palaniappan R, Chang Y, Hassan F, McDonough S, Pough M, Barr S, et al. Expression of leptospiral immunoglobulin-like protein by Leptospira interrogans and evaluation of its diagnostic potential in a kinetic ELISA. J Med Microbiol. 2004;53:975-84 pubmed publisher
    ..These findings suggest that recombinant antigen to the conserved region of LigA and LigB can differentiate between vaccinated and naturally infected animals...
  74. Asuthkar S, Velineni S, Stadlmann J, Altmann F, Sritharan M. Expression and characterization of an iron-regulated hemin-binding protein, HbpA, from Leptospira interrogans serovar Lai. Infect Immun. 2007;75:4582-91 pubmed publisher
    ..we identified and modeled a TonB-dependent outer membrane receptor protein (LB191) from the genome of Leptospira interrogans serovar Lai...
  75. Ristow P, Bourhy P, da Cruz McBride F, Figueira C, Huerre M, Ave P, et al. The OmpA-like protein Loa22 is essential for leptospiral virulence. PLoS Pathog. 2007;3:e97 pubmed
    Pathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain largely unknown. This is mainly due to the lack of tools for genetic manipulations of pathogenic species...
  76. Liao S, Sun A, Ojcius D, Wu S, Zhao J, Yan J. Inactivation of the fliY gene encoding a flagellar motor switch protein attenuates mobility and virulence of Leptospira interrogans strain Lai. BMC Microbiol. 2009;9:253 pubmed publisher
    ..A suicide plasmid for the fliY gene of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai strain Lai that was disrupted by inserting the ampicillin ..
  77. Murray G, Srikram A, Henry R, Hartskeerl R, Sermswan R, Adler B. Mutations affecting Leptospira interrogans lipopolysaccharide attenuate virulence. Mol Microbiol. 2010;78:701-9 pubmed publisher
    b>Leptospira interrogans is the causative agent of leptospirosis. Lipopolysaccharide (LPS) is the major outer membrane component of L. interrogans...
  78. Palaniappan R, Chang Y, Chang C, Pan M, Yang C, Harpending P, et al. Evaluation of lig-based conventional and real time PCR for the detection of pathogenic leptospires. Mol Cell Probes. 2005;19:111-7 pubmed
    ..Diagnostic PCR assay was also developed for the detection of pathogenic Leptospira interrogans in urine samples...
  79. Odontsetseg N, Sakoda Y, Kida H. Serological evidence of the persistence of infection with Leptospira interrogans serovar Hardjo in cattle in Mongolia. Microbiol Immunol. 2005;49:865-9 pubmed
    ..Antibodies specific to Leptospira interrogans serovar Hardjo were detected by microscopic agglutination test (MAT) at titres of 100 or more in 80...
  80. Atzingen M, Barbosa A, de Brito T, Vasconcellos S, de Morais Z, Lima D, et al. Lsa21, a novel leptospiral protein binding adhesive matrix molecules and present during human infection. BMC Microbiol. 2008;8:70 pubmed publisher
    ..Whole-genome sequencing analysis of L. interrogans allowed identification of a repertoire of putative leptospiral surface proteins...
  81. Malmstrom J, Beck M, Schmidt A, Lange V, Deutsch E, Aebersold R. Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans. Nature. 2009;460:762-5 pubmed publisher
    ..Applying the technology to the human pathogen Leptospira interrogans, a spirochete responsible for leptospirosis, we generated an absolute protein abundance scale for 83% of ..
  82. Mendes R, Von Atzingen M, de Morais Z, Gonçales A, Serrano S, Asega A, et al. The novel leptospiral surface adhesin Lsa20 binds laminin and human plasminogen and is probably expressed during infection. Infect Immun. 2011;79:4657-67 pubmed publisher
    ..The LIC11469 protein is well conserved among leptospiral strains, while LIC11030 was identified only in Leptospira interrogans. We confirmed by surface proteolysis of intact leptospires with proteinase K that these proteins are most ..
  83. Ching A, Fávaro R, Lima S, Chaves A, de Lima M, Nader H, et al. Leptospira interrogans shotgun phage display identified LigB as a heparin-binding protein. Biochem Biophys Res Commun. 2012;427:774-9 pubmed publisher
    ..Thus, heparin may act as a receptor for Leptospira to colonize and to invade the host tissue...
  84. Artiushin S, Timoney J, Nally J, Verma A. Host-inducible immunogenic sphingomyelinase-like protein, Lk73.5, of Leptospira interrogans. Infect Immun. 2004;72:742-9 pubmed
    b>Leptospira interrogans causes a variety of clinical syndromes in animals and humans...
  85. Koizumi N, Watanabe H. Leptospiral immunoglobulin-like proteins elicit protective immunity. Vaccine. 2004;22:1545-52 pubmed
    ..This paper describes the identification of two homologous immunogenic proteins, LigA-m and LigB-m, of Leptospira interrogans serovar manilae strain UP-MMC-NIID, which show strong similarity with LigA found recently in L...
  86. Matsunaga J, Werneid K, Zuerner R, Frank A, Haake D. LipL46 is a novel surface-exposed lipoprotein expressed during leptospiral dissemination in the mammalian host. Microbiology. 2006;152:3777-86 pubmed
    ..of cysteine at the lipoprotein signal peptidase cleavage site FSISC is supported by the finding that Leptospira interrogans intrinsically labels LipL46 during incubation in medium containing [(14)C]palmitate...
  87. Vieira M, Vasconcellos S, Gonçales A, de Morais Z, Nascimento A. Plasminogen acquisition and activation at the surface of leptospira species lead to fibronectin degradation. Infect Immun. 2009;77:4092-101 pubmed publisher
    ..Plasmin-coated virulent Leptospira interrogans bacteria were capable of degrading purified extracellular matrix fibronectin...
  88. Aviat F, Slamti L, Cerqueira G, Lourdault K, Picardeau M. Expanding the genetic toolbox for Leptospira species by generation of fluorescent bacteria. Appl Environ Microbiol. 2010;76:8135-42 pubmed publisher
    ..Finally, we showed that GFP can be used as a reporter to assess promoter activity in different environmental conditions. These results may facilitate further advances for studying the genetics of Leptospira spp...
  89. Lourdault K, Cerqueira G, Wunder E, Picardeau M. Inactivation of clpB in the pathogen Leptospira interrogans reduces virulence and resistance to stress conditions. Infect Immun. 2011;79:3711-7 pubmed publisher
    b>Leptospira interrogans is the causative agent of leptospirosis, which is an emerging zoonotic disease. Resistance to stress conditions is largely uncharacterized for this bacterium...
  90. Oliveira R, de Morais Z, Gonçales A, Romero E, Vasconcellos S, Nascimento A. Characterization of novel OmpA-like protein of Leptospira interrogans that binds extracellular matrix molecules and plasminogen. PLoS ONE. 2011;6:e21962 pubmed publisher
    b>Leptospira interrogans is the etiological agent of leptospirosis, a zoonotic disease of human and veterinary concern...
  91. Li S, Zhang C, Li X, Tian K, Tang G, Wang D, et al. Molecular typing of Leptospira interrogans strains isolated from Rattus tanezumi in Guizhou Province, Southwest of China. Biomed Environ Sci. 2012;25:542-8 pubmed publisher
  92. Slack A, Galloway R, Symonds M, Dohnt M, Smythe L. Reclassification of Leptospira meyeri serovar Perameles to Leptospira interrogans serovar Perameles through serological and molecular analysis: evidence of a need for changes to current procedures in Leptospira taxonomy. Int J Syst Evol Microbiol. 2009;59:1199-203 pubmed publisher
    ..meyeri strains; however, strains Lepto0213 and Bandicoot 343 had significant similarity with Leptospira interrogans strains...