legionella pneumophila

Summary

Summary: A species of gram-negative, aerobic bacteria that is the causative agent of LEGIONNAIRES' DISEASE. It has been isolated from numerous environmental sites as well as from human lung tissue, respiratory secretions, and blood.

Top Publications

  1. Zhao Y, Yang J, Shi J, Gong Y, Lu Q, Xu H, et al. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature. 2011;477:596-600 pubmed publisher
    ..Here we show that NAIP5, a BIR-domain NLR protein required for Legionella pneumophila replication in mouse macrophages, is a universal component of the flagellin-NLRC4 pathway...
  2. Mukherjee S, Liu X, Arasaki K, MCDONOUGH J, Galan J, Roy C. Modulation of Rab GTPase function by a protein phosphocholine transferase. Nature. 2011;477:103-6 pubmed publisher
    The intracellular pathogen Legionella pneumophila modulates the activity of host GTPases to direct the transport and assembly of the membrane-bound compartment in which it resides...
  3. Jayakumar D, Early J, Steinman H. Virulence phenotypes of Legionella pneumophila associated with noncoding RNA lpr0035. Infect Immun. 2012;80:4143-53 pubmed publisher
    The Philadelphia-1 strain of Legionella pneumophila, the causative organism of Legionnaires' disease, contains a recently discovered noncoding RNA, lpr0035...
  4. Fonseca M, Sauer J, Crépin S, Byrne B, Swanson M. The phtC-phtD locus equips Legionella pneumophila for thymidine salvage and replication in macrophages. Infect Immun. 2014;82:720-30 pubmed publisher
    ..by phylogenetically related intracellular gammaproteobacteria, including the opportunistic pathogen Legionella pneumophila. The location of the pht genes between the putative thymidine kinase (tdk) and phosphopentomutase (deoB) ..
  5. Mishra A, Del Campo C, Collins R, Roy C, Lambright D. The Legionella pneumophila GTPase activating protein LepB accelerates Rab1 deactivation by a non-canonical hydrolytic mechanism. J Biol Chem. 2013;288:24000-11 pubmed publisher
    ..Here, we report the transition state mimetic structure of the Legionella pneumophila GAP LepB in complex with Rab1 and describe a comprehensive structure-based mutational analysis of ..
  6. Chatfield C, Mulhern B, Viswanathan V, Cianciotto N. The major facilitator superfamily-type protein LbtC promotes the utilization of the legiobactin siderophore by Legionella pneumophila. Microbiology. 2012;158:721-35 pubmed publisher
    The Gram-negative bacterium Legionella pneumophila elaborates the siderophore legiobactin...
  7. Zhu W, Hammad L, Hsu F, Mao Y, Luo Z. Induction of caspase 3 activation by multiple Legionella pneumophila Dot/Icm substrates. Cell Microbiol. 2013;15:1783-95 pubmed publisher
    The intracellular pathogen Legionella pneumophila is able to strike a balance between the death and survival of the host cell during infection...
  8. Hoffmann C, Finsel I, Hilbi H. Pathogen vacuole purification from legionella-infected amoeba and macrophages. Methods Mol Biol. 2013;954:309-21 pubmed publisher
    b>Legionella pneumophila replicates intracellularly in environmental and immune phagocytes within a unique membrane-bound compartment, the Legionella-containing vacuole (LCV)...
  9. Jameson Lee M, Garduno R, Hoffman P. DsbA2 (27 kDa Com1-like protein) of Legionella pneumophila catalyses extracytoplasmic disulphide-bond formation in proteins including the Dot/Icm type IV secretion system. Mol Microbiol. 2011;80:835-52 pubmed publisher
    ..study, we sought to identify DSB-forming proteins required for assembly of macromolecular structures in Legionella pneumophila. Here we describe two DSB-forming proteins, one annotated as dsbA1 and the other annotated as a 27 kDa ..

More Information

Publications93

  1. Rakic A, Perić J, Foglar L. Influence of temperature, chlorine residual and heavy metals on the presence of Legionella pneumophila in hot water distribution systems. Ann Agric Environ Med. 2012;19:431-6 pubmed
    ..often occurs on the walls of plumbing systems; therefore, monitoring of opportunistic pathogens such as Legionella pneumophila (L...
  2. Sahr T, Rusniok C, Dervins Ravault D, Sismeiro O, Coppee J, Buchrieser C. Deep sequencing defines the transcriptional map of L. pneumophila and identifies growth phase-dependent regulated ncRNAs implicated in virulence. RNA Biol. 2012;9:503-19 pubmed publisher
    The bacterium Legionella pneumophila is found ubiquitously in aquatic environments and can cause a severe pneumonia in humans called Legionnaires' disease...
  3. Tan Y, Arnold R, Luo Z. Legionella pneumophila regulates the small GTPase Rab1 activity by reversible phosphorylcholination. Proc Natl Acad Sci U S A. 2011;108:21212-7 pubmed publisher
    Effectors delivered into host cells by the Legionella pneumophila Dot/Icm type IV transporter are essential for the biogenesis of the specialized vacuole that permits its intracellular growth...
  4. Kubiak X, Dervins Ravault D, Pluvinage B, Chaffotte A, Gomez Valero L, Dairou J, et al. Characterization of an acetyltransferase that detoxifies aromatic chemicals in Legionella pneumophila. Biochem J. 2012;445:219-28 pubmed publisher
    b>Legionella pneumophila is an opportunistic pathogen and the causative agent of Legionnaires' disease...
  5. Nomura H, Isshiki Y, Sakuda K, Sakuma K, Kondo S. Effects of oakmoss and its components on biofilm formation of Legionella pneumophila. Biol Pharm Bull. 2013;36:833-7 pubmed
    Oakmoss and its components are known as antibacterial agents, specifically against Legionella pneumophila. In the present study, we investigated the effects of oakmoss and its components (phenol, didepside and isochromen derivatives) on L...
  6. Stewart C, Burnside D, Cianciotto N. The surfactant of Legionella pneumophila Is secreted in a TolC-dependent manner and is antagonistic toward other Legionella species. J Bacteriol. 2011;193:5971-84 pubmed publisher
    When Legionella pneumophila grows on agar plates, it secretes a surfactant that promotes flagellum- and pilus-independent "sliding" motility. We isolated three mutants that were defective for surfactant...
  7. Mallegol J, Duncan C, Prashar A, So J, Low D, Terebeznik M, et al. Essential roles and regulation of the Legionella pneumophila collagen-like adhesin during biofilm formation. PLoS ONE. 2012;7:e46462 pubmed publisher
    Legionellosis is mostly caused by Legionella pneumophila (Lp) and is defined by a severe respiratory illness with a case fatality rate ranging from 5 to 80%...
  8. Farhat C, Mentasti M, Jacobs E, Fry N, Lück C. The N-acylneuraminate cytidyltransferase gene, neuA, is heterogenous in Legionella pneumophila strains but can be used as a marker for epidemiological typing in the consensus sequence-based typing scheme. J Clin Microbiol. 2011;49:4052-8 pubmed publisher
    Sequence-based typing (SBT) is the internationally recognized standard method for genotyping Legionella pneumophila. To date all strains of serogroup 1 (SG1) and some of SGs 2 to 14 yield a seven-allele profile and can be assigned a ..
  9. Lang C, Flieger A. Characterisation of Legionella pneumophila phospholipases and their impact on host cells. Eur J Cell Biol. 2011;90:903-12 pubmed publisher
    ..Phospholipases play a dominant role in the biology of the lung pathogen Legionella pneumophila. So far, 15 different phospholipase A-encoding genes have been identified in the L. pneumophila genome...
  10. Ku B, Lee K, Park W, Yang C, Ge J, Lee S, et al. VipD of Legionella pneumophila targets activated Rab5 and Rab22 to interfere with endosomal trafficking in macrophages. PLoS Pathog. 2012;8:e1003082 pubmed publisher
    Upon phagocytosis, Legionella pneumophila translocates numerous effector proteins into host cells to perturb cellular metabolism and immunity, ultimately establishing intracellular survival and growth. VipD of L...
  11. Arasaki K, Toomre D, Roy C. The Legionella pneumophila effector DrrA is sufficient to stimulate SNARE-dependent membrane fusion. Cell Host Microbe. 2012;11:46-57 pubmed publisher
    The intracellular bacterial pathogen Legionella pneumophila subverts host membrane transport pathways to promote fusion of vesicles exiting the endoplasmic reticulum (ER) with the pathogen-containing vacuole...
  12. Kpadeh Z, Jameson Lee M, Yeh A, Chertihin O, Shumilin I, Dey R, et al. Disulfide bond oxidoreductase DsbA2 of Legionella pneumophila exhibits protein disulfide isomerase activity. J Bacteriol. 2013;195:1825-33 pubmed publisher
    The extracytoplasmic assembly of the Dot/Icm type IVb secretion system (T4SS) of Legionella pneumophila is dependent on correct disulfide bond (DSB) formation catalyzed by a novel and essential disulfide bond oxidoreductase DsbA2 and not ..
  13. Cheng W, Yin K, Lu D, Li B, Zhu D, Chen Y, et al. Structural insights into a unique Legionella pneumophila effector LidA recognizing both GDP and GTP bound Rab1 in their active state. PLoS Pathog. 2012;8:e1002528 pubmed publisher
    The intracellular pathogen Legionella pneumophila hijacks the endoplasmic reticulum (ER)-derived vesicles to create an organelle designated Legionella-containing vacuole (LCV) required for bacterial replication...
  14. Cho M, Kim H, An D, Lee M, Noh S, Kim M, et al. Comparison of sputum and nasopharyngeal swab specimens for molecular diagnosis of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila. Ann Lab Med. 2012;32:133-8 pubmed publisher
    ..swabs (NPS) for use in detection of Mycoplasma pneumoniae (MP), Chlamydophila pneumoniae (CP), and Legionella pneumophila (LP), using Seeplex PneumoBacter ACE Detection Assay (PneumoBacter; Seegene)...
  15. Amyot W, Dejesus D, Isberg R. Poison domains block transit of translocated substrates via the Legionella pneumophila Icm/Dot system. Infect Immun. 2013;81:3239-52 pubmed publisher
    b>Legionella pneumophila uses the Icm/Dot type 4B secretion system (T4BSS) to deliver translocated protein substrates to the host cell, promoting replication vacuole formation...
  16. Chen Y, Tascón I, Neunuebel M, Pallara C, Brady J, Kinch L, et al. Structural basis for Rab1 de-AMPylation by the Legionella pneumophila effector SidD. PLoS Pathog. 2013;9:e1003382 pubmed publisher
    ..the only known virulence protein with de-AMPylation activity is SidD from the human pathogen Legionella pneumophila. SidD de-AMPylates mammalian Rab1, a small GTPase involved in secretory vesicle transport, thereby ..
  17. Tiaden A, Kessler A, Hilbi H. Analysis of legionella infection by flow cytometry. Methods Mol Biol. 2013;954:233-49 pubmed publisher
    b>Legionella pneumophila infects and replicates in environmental protozoa and metazoan macrophages within a specific vacuole. The infection of phagocytes by L...
  18. Nasrallah G, Riveroll A, Chong A, Murray L, Lewis P, Garduno R. Legionella pneumophila requires polyamines for optimal intracellular growth. J Bacteriol. 2011;193:4346-60 pubmed publisher
    The Gram-negative intracellular pathogen Legionella pneumophila replicates in a membrane-bound compartment known as the Legionella-containing vacuole (LCV), into which it abundantly releases its chaperonin, HtpB...
  19. Pereira M, Morgantetti G, Massis L, Horta C, Hori J, Zamboni D. Activation of NLRC4 by flagellated bacteria triggers caspase-1-dependent and -independent responses to restrict Legionella pneumophila replication in macrophages and in vivo. J Immunol. 2011;187:6447-55 pubmed publisher
    ..In this study, we used mice deficient for the inflammasome components in addition to wild-type (WT) Legionella pneumophila or bacteria deficient for flagellin (flaA) or motility (fliI) to assess the pathways responsible for NLRC4-..
  20. von Baum H, Luck C. [Community-acquired Legionella pneumonia : data from the CAPNETZ study]. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2011;54:688-92 pubmed publisher
    ..More than 51 different species of Legionella have been identified; however, most pneumonias are caused by Legionella pneumophila serogroup 1. Legionnaire's disease has an incidence of about 4% in Germany...
  21. Costa J, d Avó A, da Costa M, Veríssimo A. Molecular evolution of key genes for type II secretion in Legionella pneumophila. Environ Microbiol. 2012;14:2017-33 pubmed publisher
    Given the role of type II protein secretion system (T2S) in the ecology and pathogenesis of Legionella pneumophila, it is possible that this system is a target for adaptive evolution. The population genetic structure of L...
  22. Helbig J, Jacobs E, Luck C. Legionella pneumophila urinary antigen subtyping using monoclonal antibodies as a tool for epidemiological investigations. Eur J Clin Microbiol Infect Dis. 2012;31:1673-7 pubmed publisher
    ..pneumophila strains, which are widespread contaminants of water systems, but represent the minority of patient isolates. ..
  23. Tiaden A, Hilbi H. ?-Hydroxyketone synthesis and sensing by Legionella and Vibrio. Sensors (Basel). 2012;12:2899-919 pubmed publisher
    ..the ?-hydroxyketones (AHKs), is produced and detected by the water-borne opportunistic pathogens Legionella pneumophila and Vibrio cholerae, which cause Legionnaires' disease and cholera, respectively...
  24. Euser S, Nagelkerke N, Schuren F, Jansen R, Den Boer J. Genome analysis of Legionella pneumophila strains using a mixed-genome microarray. PLoS ONE. 2012;7:e47437 pubmed publisher
    ..Mixed-genome microarray data can be used to perform a comparative-genome analysis of strain collections, and advanced statistical approaches, such as the Random Forest algorithm are available to process these data...
  25. O Connor T, Boyd D, Dorer M, Isberg R. Aggravating genetic interactions allow a solution to redundancy in a bacterial pathogen. Science. 2012;338:1440-4 pubmed publisher
    ..We used this technique to resolve the network of proteins secreted by the bacterium Legionella pneumophila to promote intracellular growth, a critical determinant of pathogenicity of this organism...
  26. Wuerz T, Mooney O, Keynan Y. Legionella pneumophila serotype 1 pneumonia in patient receiving adalimumab. Emerg Infect Dis. 2012;18:1872-4 pubmed publisher
    We describe a case of severe pneumonia caused by Legionella pneumophila serotype 1 in a woman receiving the tumor necrosis factor-? antagonist to treat rheumatoid arthritis...
  27. Kirschner A, Rameder A, Schrammel B, Indra A, Farnleitner A, Sommer R. Development of a new CARD-FISH protocol for quantification of Legionella pneumophila and its application in two hospital cooling towers. J Appl Microbiol. 2012;112:1244-56 pubmed publisher
    Open cooling towers are frequent sources of infections with Legionella pneumophila. The gold standard for the detection of Leg. pneumophila is based on cultivation lasting up to 10 days and detecting only culturable cells...
  28. Lautner M, Schunder E, Herrmann V, Heuner K. Regulation, integrase-dependent excision, and horizontal transfer of genomic islands in Legionella pneumophila. J Bacteriol. 2013;195:1583-97 pubmed publisher
    b>Legionella pneumophila is a Gram-negative freshwater agent which multiplies in specialized nutrient-rich vacuoles of amoebae. When replicating in human alveolar macrophages, Legionella can cause Legionnaires' disease...
  29. Allombert J, Fuche F, Michard C, Doublet P. Molecular mimicry and original biochemical strategies for the biogenesis of a Legionella pneumophila replicative niche in phagocytic cells. Microbes Infect. 2013;15:981-8 pubmed publisher
    b>Legionella pneumophila is a paradigm of highly adapted intravacuolar pathogens that acquired the rare ability to replicate within a phagocytic cell...
  30. Kaku N, Yanagihara K, Morinaga Y, Sato T, Nakashima M, Sakai T, et al. Detection of Legionella pneumophila serogroup 1 in blood cultures from a patient treated with tumor necrosis factor-alpha inhibitor. J Infect Chemother. 2013;19:166-70 pubmed publisher
    ..Thereafter, we received positive urinary antigen test findings for Legionella pneumophila. After hospitalization, hypoxia was progressed and hypotension was emerged...
  31. Fontana M, Banga S, Barry K, Shen X, Tan Y, Luo Z, et al. Secreted bacterial effectors that inhibit host protein synthesis are critical for induction of the innate immune response to virulent Legionella pneumophila. PLoS Pathog. 2011;7:e1001289 pubmed publisher
    The intracellular bacterial pathogen Legionella pneumophila causes an inflammatory pneumonia called Legionnaires' Disease. For virulence, L...
  32. Abdel Nour M, Duncan C, Low D, Guyard C. Biofilms: the stronghold of Legionella pneumophila. Int J Mol Sci. 2013;14:21660-75 pubmed publisher
    Legionellosis is mostly caused by Legionella pneumophila and is defined as a severe respiratory illness with a case fatality rate ranging from 5% to 80%. L. pneumophila is ubiquitous in natural and anthropogenic water systems. L...
  33. Goody P, Heller K, Oesterlin L, Müller M, Itzen A, Goody R. Reversible phosphocholination of Rab proteins by Legionella pneumophila effector proteins. EMBO J. 2012;31:1774-84 pubmed publisher
    The Legionella pneumophila protein AnkX that is injected into infected cells by a Type IV secretion system transfers a phosphocholine group from CDP-choline to a serine in the Rab1 and Rab35 GTPase Switch II regions...
  34. Neunuebel M, Chen Y, Gaspar A, Backlund P, Yergey A, Machner M. De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila. Science. 2011;333:453-6 pubmed publisher
    The bacterial pathogen Legionella pneumophila exploits host cell vesicle transport by transiently manipulating the activity of the small guanosine triphosphatase (GTPase) Rab1...
  35. Ohtsuka S, Ishii Y, Matsuyama M, Ano S, Morishima Y, Yanagawa T, et al. SQSTM1/p62/A170 regulates the severity of Legionella pneumophila pneumonia by modulating inflammasome activity. Eur J Immunol. 2014;44:1084-92 pubmed publisher
    ..In this study, we investigated the role of SQSTM1 in host responses to Legionella pneumophila, an intra-cellular pathogen that infects macrophages, in both an SQSTM1-deficient (SQSTM1(-/-) ) mouse ..
  36. Weissenmayer B, Prendergast J, Lohan A, Loftus B. Sequencing illustrates the transcriptional response of Legionella pneumophila during infection and identifies seventy novel small non-coding RNAs. PLoS ONE. 2011;6:e17570 pubmed publisher
    ..b>Legionella pneumophila is a gram-negative facultative intracellular human pathogen with a distinct biphasic lifestyle...
  37. Tao L, Zhu W, Hu B, Qu J, Luo Z. Induction of rapid cell death by an environmental isolate of Legionella pneumophila in mouse macrophages. Infect Immun. 2013;81:3077-88 pubmed publisher
    b>Legionella pneumophila, the etiological agent for Legionnaires' disease, is ubiquitous in the aqueous environment, where it replicates as an intracellular parasite of free-living protozoa. Our understanding of L...
  38. Neunuebel M, Machner M. The taming of a Rab GTPase by Legionella pneumophila. Small Gtpases. 2012;3:28-33 pubmed publisher
    ..b>Legionella pneumophila is an intravacuolar pathogen that survives inside host cells by manipulating protein trafficking pathways ..
  39. Visca P, D Arezzo S, Ramisse F, Gelfand Y, Benson G, Vergnaud G, et al. Investigation of the population structure of Legionella pneumophila by analysis of tandem repeat copy number and internal sequence variation. Microbiology. 2011;157:2582-94 pubmed publisher
    The population structure of the species Legionella pneumophila was investigated by multilocus variable number of tandem repeats (VNTR) analysis (MLVA) and sequencing of three VNTRs (Lpms01, Lpms04 and Lpms13) in selected strains...
  40. Choy A, Dancourt J, Mugo B, O Connor T, Isberg R, Melia T, et al. The Legionella effector RavZ inhibits host autophagy through irreversible Atg8 deconjugation. Science. 2012;338:1072-6 pubmed publisher
    ..Here, we found that the intracellular pathogen Legionella pneumophila could interfere with autophagy by using the bacterial effector protein RavZ to directly uncouple Atg8 ..
  41. Jager J, Marwitz S, Tiefenau J, Rasch J, Shevchuk O, Kugler C, et al. Human lung tissue explants reveal novel interactions during Legionella pneumophila infections. Infect Immun. 2014;82:275-85 pubmed publisher
    ..Therefore, we developed and applied a novel model for Legionella pneumophila infection comprising living human lung tissue. We stimulated lung explants with L...
  42. Case C, Kohler L, Lima J, Strowig T, de Zoete M, Flavell R, et al. Caspase-11 stimulates rapid flagellin-independent pyroptosis in response to Legionella pneumophila. Proc Natl Acad Sci U S A. 2013;110:1851-6 pubmed publisher
    ..caspase-1 activation pathway that does not require NAIP5 or NRLC4 is induced by the intracellular pathogen Legionella pneumophila. Here we demonstrate that this pathway requires caspase-11...
  43. Tyson J, Pearce M, Vargas P, Bagchi S, Mulhern B, Cianciotto N. Multiple Legionella pneumophila Type II secretion substrates, including a novel protein, contribute to differential infection of the amoebae Acanthamoeba castellanii, Hartmannella vermiformis, and Naegleria lovaniensis. Infect Immun. 2013;81:1399-410 pubmed publisher
    Type II protein secretion (T2S) by Legionella pneumophila is required for intracellular infection of host cells, including macrophages and the amoebae Acanthamoeba castellanii and Hartmannella vermiformis...
  44. Barry K, Fontana M, Portman J, Dugan A, Vance R. IL-1? signaling initiates the inflammatory response to virulent Legionella pneumophila in vivo. J Immunol. 2013;190:6329-39 pubmed publisher
    b>Legionella pneumophila is an intracellular bacterial pathogen that is the cause of a severe pneumonia in humans called Legionnaires' disease. A key feature of L...
  45. Prashar A, Bhatia S, Tabatabaeiyazdi Z, Duncan C, Garduno R, Tang P, et al. Mechanism of invasion of lung epithelial cells by filamentous Legionella pneumophila. Cell Microbiol. 2012;14:1632-55 pubmed publisher
    ..While the filamentous forms of Legionella pneumophila (Lp) have been reported in patient samples since the first description of legionellosis, their role in ..
  46. Ge J, Gong Y, Xu Y, Shao F. Preventing bacterial DNA release and absent in melanoma 2 inflammasome activation by a Legionella effector functioning in membrane trafficking. Proc Natl Acad Sci U S A. 2012;109:6193-8 pubmed publisher
    b>Legionella pneumophila, the causative agent of Legionnaires' pneumonia, resides in a distinct vacuole structure called Legionella-containing vacuole (LCV)...
  47. Krøjgaard L, Krogfelt K, Albrechtsen H, Uldum S. Detection of Legionella by quantitative-polymerase chain reaction (qPCR) for monitoring and risk assessment. BMC Microbiol. 2011;11:254 pubmed publisher
    ..The aims were to investigate the usefulness of qPCR compared to culture for monitoring remedial actions for elimination of Legionella bacteria and as a tool for risk assessment...
  48. Keserue H, Baumgartner A, Felleisen R, Egli T. Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry. Microb Biotechnol. 2012;5:753-63 pubmed publisher
    We developed a rapid detection method for Legionella pneumophila (Lp) by filtration, immunomagnetic separation, double fluorescent staining, and flow cytometry (IMS-FCM method)...
  49. Zhu W, Banga S, Tan Y, Zheng C, Stephenson R, Gately J, et al. Comprehensive identification of protein substrates of the Dot/Icm type IV transporter of Legionella pneumophila. PLoS ONE. 2011;6:e17638 pubmed publisher
    A large number of proteins transferred by the Legionella pneumophila Dot/Icm system have been identified by various strategies...
  50. Dolezal P, Aili M, Tong J, Jiang J, Marobbio C, Marobbio C, et al. Legionella pneumophila secretes a mitochondrial carrier protein during infection. PLoS Pathog. 2012;8:e1002459 pubmed publisher
    ..a novel MCF member, termed Legionellanucleotide carrier Protein (LncP), encoded in the genome of Legionella pneumophila, the causative agent of Legionnaire's disease...
  51. Sobral D, Le Cann P, Gerard A, Jarraud S, Lebeau B, Loisy Hamon F, et al. High-throughput typing method to identify a non-outbreak-involved Legionella pneumophila strain colonizing the entire water supply system in the town of Rennes, France. Appl Environ Microbiol. 2011;77:6899-907 pubmed publisher
    ..outbreaks occurred in the city of Rennes, France, during the past decade, requiring in-depth monitoring of Legionella pneumophila in the water network and the cooling towers in the city...
  52. Buchrieser C, Charpentier X. Induction of competence for natural transformation in Legionella pneumophila and exploitation for mutant construction. Methods Mol Biol. 2013;954:183-95 pubmed publisher
    ..a combined temperature and aeration shift, which may be exploited for the construction of mutants in Legionella pneumophila. This approach seems to be less prone to the appearance of secondary mutations during mutant construction ..
  53. Koubar M, Rodier M, Garduno R, Frère J. Passage through Tetrahymena tropicalis enhances the resistance to stress and the infectivity of Legionella pneumophila. FEMS Microbiol Lett. 2011;325:10-5 pubmed publisher
    b>Legionella pneumophila is a gram-negative bacterium prevalent in fresh water which accidentally infects humans and is responsible for the disease called legionellosis. Intracellular growth of L...
  54. Kimizuka Y, Kimura S, Saga T, Ishii M, Hasegawa N, Betsuyaku T, et al. Roles of interleukin-17 in an experimental Legionella pneumophila pneumonia model. Infect Immun. 2012;80:1121-7 pubmed publisher
    ..Although Legionella pneumophila, an intracellular bacterium found widely in the environment, often causes a serious and life-threatening ..
  55. Sutherland M, Nguyen T, Tseng V, Vogel J. The Legionella IcmSW complex directly interacts with DotL to mediate translocation of adaptor-dependent substrates. PLoS Pathog. 2012;8:e1002910 pubmed publisher
    b>Legionella pneumophila is a Gram-negative bacterium that replicates within human alveolar macrophages by evasion of the host endocytic pathway through the formation of a replicative vacuole...
  56. Tan Y, Luo Z. Legionella pneumophila SidD is a deAMPylase that modifies Rab1. Nature. 2011;475:506-9 pubmed publisher
    b>Legionella pneumophila actively modulates host vesicle trafficking pathways to facilitate its intracellular replication with effectors translocated by the Dot/Icm type IV secretion system (T4SS)...
  57. Guy S, Worth L, Thursky K, Francis P, Slavin M. Legionella pneumophila lung abscess associated with immune suppression. Intern Med J. 2011;41:715-21 pubmed publisher
    ..We describe Legionella pneumophila pneumonia and abscess formation in an immunosuppressed patient receiving corticosteroid therapy for ..
  58. Weber S, Dolinsky S, Hilbi H. Interactions of legionella effector proteins with host phosphoinositide lipids. Methods Mol Biol. 2013;954:367-80 pubmed publisher
    By means of the Icm/Dot type IV secretion system Legionella pneumophila translocates several effector proteins into host cells, where they anchor to the cytoplasmic face of the LCV membrane by binding to phosphoinositide (PI) lipids...
  59. Jank T, Böhmer K, Tzivelekidis T, Schwan C, Belyi Y, Aktories K. Domain organization of Legionella effector SetA. Cell Microbiol. 2012;14:852-68 pubmed publisher
    b>Legionella pneumophila is a human pathogen causing severe pneumonia called Legionnaires' disease. Multiple Legionella effectors are type IV-secreted into the host cell to establish a specific vesicular compartment for pathogen replication...
  60. Schalk J, Docters van Leeuwen A, Lodder W, De Man H, Euser S, Den Boer J, et al. Isolation of Legionella pneumophila from pluvial floods by amoebal coculture. Appl Environ Microbiol. 2012;78:4519-21 pubmed publisher
    Viable Legionella pneumophila bacteria were isolated by amoebal coculture from pluvial floods after intense rainfall and from water collected at sewage treatment plants. Several isolated L...
  61. Grant G. Kinetic evidence of a noncatalytic substrate binding site that regulates activity in Legionella pneumophila L-serine dehydratase. Biochemistry. 2012;51:6961-7 pubmed publisher
    The L-serine dehydratase from Legionella pneumophila (lpLSD) has recently been shown to contain a domain (? domain) that has a high degree of structural homology with the ASB domain of d-3-phosphoglycerate dehydrogenase (PGDH) from ..
  62. Parthuisot N, Binet M, Touron Bodilis A, Pougnard C, Lebaron P, Baudart J. Total and viable Legionella pneumophila cells in hot and natural waters as measured by immunofluorescence-based assays and solid-phase cytometry. Appl Environ Microbiol. 2011;77:6225-32 pubmed publisher
    A new method was developed for the rapid and sensitive detection of viable Legionella pneumophila. The method combines specific immunofluorescence (IF) staining using monoclonal antibodies with a bacterial viability marker (ChemChrome V6 ..
  63. Al Quadan T, Price C, Abu Kwaik Y. Exploitation of evolutionarily conserved amoeba and mammalian processes by Legionella. Trends Microbiol. 2012;20:299-306 pubmed publisher
    b>Legionella pneumophila proliferates within various protists and metazoan cells, where a cadre of ?300 effectors is injected into the host cell by the defect in organelle trafficking/intracellular multiplication (Dot/Icm) type IVB ..
  64. Belyi Y, Tartakovskaya D, Tais A, Fitzke E, Tzivelekidis T, Jank T, et al. Elongation factor 1A is the target of growth inhibition in yeast caused by Legionella pneumophila glucosyltransferase Lgt1. J Biol Chem. 2012;287:26029-37 pubmed publisher
    ..One effector of Legionella pneumophila is the glucosyltransferase Lgt1, which modifies serine 53 in mammalian elongation factor 1A (eEF1A), ..
  65. Zheng H, Chatfield C, Liles M, Cianciotto N. Secreted pyomelanin of Legionella pneumophila promotes bacterial iron uptake and growth under iron-limiting conditions. Infect Immun. 2013;81:4182-91 pubmed publisher
    Iron acquisition is critical to the growth and virulence of Legionella pneumophila. Previously, we found that L. pneumophila uses both a ferrisiderophore pathway and ferrous iron transport to obtain iron...
  66. Garcia Vidal C, Labori M, Viasus D, Simonetti A, García Somoza D, Dorca J, et al. Rainfall is a risk factor for sporadic cases of Legionella pneumophila pneumonia. PLoS ONE. 2013;8:e61036 pubmed publisher
    ..Physicians should carefully consider Legionella pneumonia when selecting diagnostic tests and antimicrobial therapy for patients presenting with CAP after periods of rainfall...
  67. Al Bana B, Haddad M, Garduno R. Stationary phase and mature infectious forms of Legionella pneumophila produce distinct viable but non-culturable cells. Environ Microbiol. 2014;16:382-95 pubmed publisher
    b>Legionella pneumophila is an intracellular bacterial parasite of freshwater protozoa and an accidental waterborne human pathogen. L.?pneumophila is highly pleomorphic showing several forms that differentiate within its developmental cycle...
  68. Knez K, Janssen K, Spasic D, Declerck P, Vanysacker L, Denis C, et al. Spherical nucleic acid enhanced FO-SPR DNA melting for detection of mutations in Legionella pneumophila. Anal Chem. 2013;85:1734-42 pubmed publisher
    ..The concept was demonstrated for the analysis of 9 different serogroups of the bacterium Legionella pneumophila, a common human pathogen responsible for atypical pneumonia...
  69. Cao B, Yao F, Liu X, Feng L, Wang L. Development of a DNA microarray method for detection and identification of all 15 distinct O-antigen forms of Legionella pneumophila. Appl Environ Microbiol. 2013;79:6647-54 pubmed publisher
    ..Of the 50 species, 20 are pathogenic, and Legionella pneumophila is responsible for the great majority (approximately 90%) of the Legionnaires' disease cases that occur...
  70. Chen Y, Machner M. Targeting of the small GTPase Rab6A' by the Legionella pneumophila effector LidA. Infect Immun. 2013;81:2226-35 pubmed publisher
    When the bacterium Legionella pneumophila, the causative agent of Legionnaires' disease, is phagocytosed by alveolar macrophages, it delivers a large number of effector proteins through its Dot/Icm type IV secretion system into the host ..
  71. Varner T, Bookstaver P, Rudisill C, Albrecht H. Role of rifampin-based combination therapy for severe community-acquired Legionella pneumophila pneumonia. Ann Pharmacother. 2011;45:967-76 pubmed publisher
    To review the literature concerning the role of rifampin in the combination treatment of Legionella pneumophila pneumonia.
  72. Wedege E, Bolstad K, Borgen K, Fritzsønn E, Caugant D. Molecular characterization of clinical and environmental isolates of Legionella pneumophila in Norway, 2001-2008. Scand J Infect Dis. 2013;45:59-64 pubmed publisher
    The aims of the study were to determine the molecular characteristics of a collection of Legionella pneumophila isolates from 45 cases with Legionnaires' disease and from 96 environmental samples, received by the national reference ..
  73. Belyi Y, Jank T, Aktories K. Cytotoxic glucosyltransferases of Legionella pneumophila. Curr Top Microbiol Immunol. 2013;376:211-26 pubmed publisher
    ..Here, our current knowledge about both groups of L. pneumophila glycosylating effectors is reviewed. ..
  74. Duncan C, Prashar A, So J, Tang P, Low D, Terebiznik M, et al. Lcl of Legionella pneumophila is an immunogenic GAG binding adhesin that promotes interactions with lung epithelial cells and plays a crucial role in biofilm formation. Infect Immun. 2011;79:2168-81 pubmed publisher
    Legionellosis is mostly caused by Legionella pneumophila and is defined by a severe respiratory illness with a case fatality rate ranging from 5 to 80%. In vitro and in vivo, interactions of L...
  75. Price C, Al Quadan T, Santic M, Rosenshine I, Abu Kwaik Y. Host proteasomal degradation generates amino acids essential for intracellular bacterial growth. Science. 2011;334:1553-7 pubmed publisher
    b>Legionella pneumophila proliferates in environmental amoeba and human cells within the Legionella-containing vacuole (LCV). The exported AnkB F-box effector of L...
  76. Sasaki T, Mizuguchi S, Honda K. Growth inhibitory effects of anthranilic acid and its derivatives against Legionella pneumophila. J Biosci Bioeng. 2012;113:726-9 pubmed publisher
    b>Legionella pneumophila is the principal etiologic agent of Legionnaires' disease. We found that the growth of L...
  77. Rolando M, Sanulli S, Rusniok C, Gomez Valero L, Bertholet C, Sahr T, et al. Legionella pneumophila effector RomA uniquely modifies host chromatin to repress gene expression and promote intracellular bacterial replication. Cell Host Microbe. 2013;13:395-405 pubmed publisher
    ..We find that the intracellular bacterial pathogen Legionella pneumophila uses a Dot/Icm type IV secreted effector, RomA, to uniquely modify the host chromatin landscape...
  78. Amaro F, Gilbert J, Owens S, Trimble W, Shuman H. Whole-genome sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H. J Bacteriol. 2012;194:1613-4 pubmed publisher
    We present the genomic sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H (ATCC 43290), a clinical isolate from the Colorado Department of Health, Denver, CO...
  79. Rolando M, Buchrieser C. Post-translational modifications of host proteins by Legionella pneumophila: a sophisticated survival strategy. Future Microbiol. 2012;7:369-81 pubmed publisher
    ..b>Legionella pneumophila, an intracellular pathogen that coevolved with protozoan hosts, encodes a large arsenal of secreted ..
  80. Rao C, Benhabib H, Ensminger A. Phylogenetic reconstruction of the Legionella pneumophila Philadelphia-1 laboratory strains through comparative genomics. PLoS ONE. 2013;8:e64129 pubmed publisher
    Over 20 years ago, two groups independently domesticated Legionella pneumophila from a clinical isolate of bacteria collected during the first recognized outbreak of Legionnaires' disease (at the 1976 American Legion's convention in ..
  81. Touron Bodilis A, Pougnard C, Frenkiel Lebossé H, Hallier Soulier S. Usefulness of real-time PCR as a complementary tool to the monitoring of Legionella spp. and Legionella pneumophila by culture in industrial cooling systems. J Appl Microbiol. 2011;111:499-510 pubmed publisher
    ..and Legionella pneumophila in industrial cooling systems and its ability to anticipate culture trends by the French standard method (..
  82. Viner R, Chetrit D, Ehrlich M, Segal G. Identification of two Legionella pneumophila effectors that manipulate host phospholipids biosynthesis. PLoS Pathog. 2012;8:e1002988 pubmed publisher
    The intracellular pathogen Legionella pneumophila translocates a large number of effector proteins into host cells via the Icm/Dot type-IVB secretion system. Some of these effectors were shown to cause lethal effect on yeast growth...
  83. Liu M, Haenssler E, Uehara T, Losick V, Park J, Isberg R. The Legionella pneumophila EnhC protein interferes with immunostimulatory muramyl peptide production to evade innate immunity. Cell Host Microbe. 2012;12:166-76 pubmed publisher
    ..Upon Legionella pneumophila infection of macrophages, the cytosolic PRR Nod1 recognizes anhydro-disaccharide-tetrapeptide (anhDSTP) ..
  84. Levet Paulo M, Lazzaroni J, Gilbert C, Atlan D, Doublet P, Vianney A. The atypical two-component sensor kinase Lpl0330 from Legionella pneumophila controls the bifunctional diguanylate cyclase-phosphodiesterase Lpl0329 to modulate bis-(3'-5')-cyclic dimeric GMP synthesis. J Biol Chem. 2011;286:31136-44 pubmed publisher
    ..The Legionella pneumophila Lens genome displays 22 GGDEF/EAL domain-encoding genes...