comamonas testosteroni

Summary

Summary: A species of gram-negative, aerobic rods formerly called Pseudomonas testosteroni. It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or maleate as carbon sources.

Top Publications

  1. Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T. Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441. Appl Environ Microbiol. 2005;71:5275-81 pubmed
    b>Comamonas testosteroni TA441 utilizes testosterone via aromatization of the A ring followed by meta-cleavage of the ring...
  2. Chang Y, Huang T, Chuang L, Hwang C. Role of S114 in the NADH-induced conformational change and catalysis of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni. Biochim Biophys Acta. 2009;1794:1459-66 pubmed publisher
    ..Substitution of alanine for S114 eliminates the hydrogen bonding interaction with P185, causing a conformational change in a nonproductive binding of NADH and a significant loss of activity...
  3. Hwang C, Chang Y, Hsu C, Hsu H, Li C, Pon H. Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni. J Biol Chem. 2005;280:3522-8 pubmed
    3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) from Comamonas testosteroni, a short chain dehydrogenase/reductase, catalyzes the oxidation of androsterone with NAD+ to form androstanedione and NADH...
  4. Göhler A, Xiong G, Paulsen S, Trentmann G, Maser E. Testosterone-inducible regulator is a kinase that drives steroid sensing and metabolism in Comamonas testosteroni. J Biol Chem. 2008;283:17380-90 pubmed publisher
    ..dehydrogenase/carbonyl reductase (3alpha-HSD/CR) as a reporter system to study steroid signaling in Comamonas testosteroni. In previous investigations we cloned and characterized the 3alpha-HSD/CR-encoding gene, hsdA...
  5. Thakor N, Trivedi U, Patel K. Biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl-PHAs) by Comamonas testosteroni during cultivation on vegetable oils. Bioresour Technol. 2005;96:1843-50 pubmed
    b>Comamonas testosteroni has been studied for its ability to synthesize and accumulate medium chain length poly(3-hydroxyalkanoates) (mcl-PHAs) during cultivation on vegetable oils available in the local market...
  6. Loffhagen N, Härtig C, Harms H. Impact of membrane fatty acid composition on the uncoupling sensitivity of the energy conservation of Comamonas testosteroni ATCC 17454. Appl Microbiol Biotechnol. 2006;70:618-24 pubmed
    The fatty acid composition of pyruvate-grown Comamonas testosteroni ATCC 17454 was analyzed after growth at 30 and 20 degrees C and after half-maximum growth inhibition caused by different membrane-active chemicals at 30 degrees C...
  7. Yoshida M, Hiromoto T, Hosokawa K, Yamaguchi H, Fujiwara S. Ligand specificity of MobR, a transcriptional regulator for the 3-hydroxybenzoate hydroxylase gene of Comamonas testosteroni KH122-3s. Biochem Biophys Res Commun. 2007;362:275-80 pubmed
    MobR from Comamonas testosteroni KH122-3s is a member of the MarR family of transcriptional regulators and functions as a repressor for the mobA gene that encodes a 3-hydroxybenzoate 4-hydroxylase...
  8. Hiromoto T, Fujiwara S, Hosokawa K, Yamaguchi H. Crystal structure of 3-hydroxybenzoate hydroxylase from Comamonas testosteroni has a large tunnel for substrate and oxygen access to the active site. J Mol Biol. 2006;364:878-96 pubmed
    The 3-hydroxybenzoate hydroxylase (MHBH) from Comamonas testosteroni KH122-3s is a single-component flavoprotein monooxygenase, a member of the glutathione reductase (GR) family...
  9. Hiraoka Y, Yamada T, Tone K, Futaesaku Y, Kimbara K. Flow cytometry analysis of changes in the DNA content of the polychlorinated biphenyl degrader Comamonas testosteroni TK102: effect of metabolites on cell-cell separation. Appl Environ Microbiol. 2002;68:5104-12 pubmed
    ..was used to monitor changes in the DNA content of the polychlorinated biphenyl (PCB)-degrading bacterium Comamonas testosteroni TK102 during growth in the presence or absence of PCBs...

More Information

Publications76

  1. Teramoto M, Ohnishi K, Harayama S, Watanabe K. An AraC/XylS family member at a high level in a hierarchy of regulators for phenol-metabolizing enzymes in Comamonas testosteroni R5. J Bacteriol. 2002;184:3941-6 pubmed
    b>Comamonas testosteroni strain R5 expresses a higher level of phenol-oxygenating activity than any other bacterial strain so far characterized...
  2. Mampel J, Maier E, Tralau T, Ruff J, Benz R, Cook A. A novel outer-membrane anion channel (porin) as part of a putatively two-component transport system for 4-toluenesulphonate in Comamonas testosteroni T-2. Biochem J. 2004;383:91-9 pubmed
    Inducible mineralization of TSA (4-toluenesulphonate) by Comamonas testosteroni T-2 is initiated by a secondary transport system, followed by oxygenation and oxidation by TsaMBCD to 4-sulphobenzoate under the regulation of TsaR and TsaQ...
  3. Horinouchi M, Hayashi T, Kudo T. The genes encoding the hydroxylase of 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione in steroid degradation in Comamonas testosteroni TA441. J Steroid Biochem Mol Biol. 2004;92:143-54 pubmed
    Steroid degradation genes of Comamonas testosteroni TA441 are encoded in at least two gene clusters: one containing the meta-cleavage enzyme gene tesB; and another consisting of ORF18, 17, tesI, H, ORF11, 12, and tesDEFG...
  4. Nam G, Cha S, Yun Y, Oh Y, Hong B, Lee H, et al. The conserved cis-Pro39 residue plays a crucial role in the proper positioning of the catalytic base Asp38 in ketosteroid isomerase from Comamonas testosteroni. Biochem J. 2003;375:297-305 pubmed
    KSI (ketosteroid isomerase) from Comamonas testosteroni is a homodimeric enzyme that catalyses the allylic isomerization of Delta5-3-ketosteroids to their conjugated Delta4-isomers at a reaction rate equivalent to the diffusion-controlled ..
  5. Horinouchi M, Hayashi T, Koshino H, Yamamoto T, Kudo T. Gene encoding the hydrolase for the product of the meta-cleavage reaction in testosterone degradation by Comamonas testosteroni. Appl Environ Microbiol. 2003;69:2139-52 pubmed
    ..Downstream of tesD, we identified tesE, F, and G, which encode for enzymes that degrade one of the products of 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oic acid converted by TesD...
  6. Pepper I, Gentry T, Newby D, Roane T, Josephson K. The role of cell bioaugmentation and gene bioaugmentation in the remediation of co-contaminated soils. Environ Health Perspect. 2002;110 Suppl 6:943-6 pubmed
    ..Cell bioaugmentation was further demonstrated when (Comamonas testosteroni was used to enhance biodegradation of 3-CB in Madera soil...
  7. Xiong G, Maser E. Regulation of the steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase gene in Comamonas testosteroni. J Biol Chem. 2001;276:9961-70 pubmed
    The Comamonas testosteroni 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase gene (hsdA) codes for an adaptive enzyme in the degradation of steroid compounds...
  8. Tralau T, Cook A, Ruff J. Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for p-toluenesulfonate degradation in Comamonas testosteroni T-2. Appl Environ Microbiol. 2001;67:1508-16 pubmed
    The catabolic IncP1beta plasmid pTSA from Comamonas testosteroni T-2 was mapped by subtractive analysis of restriction digests, by sequencing outwards from the tsa operon (toluenesulfonate degradation), and by generating overlapping, long-..
  9. Bathe S. Conjugal transfer of plasmid pNB2 to activated sludge bacteria leads to 3-chloroaniline degradation in enrichment cultures. Lett Appl Microbiol. 2004;38:527-31 pubmed
    ..The involvement of the aniline-degradative plasmid pNB2 in degradation of 3-chloroaniline (3-CA) was investigated...
  10. Petrounia I, Blotny G, Pollack R. Binding of 2-naphthols to D38E mutants of 3-oxo-Delta 5-steroid isomerase: variation of ligand ionization state with the nature of the electrophilic component. Biochemistry. 2000;39:110-6 pubmed
    ..Removal of either one of these H-bond donors (Tyr-14 or Asp-99) causes destabilization of the anion and results in a dienol enzyme-intermediate complex rather than a dienolate...
  11. Horinouchi M, Hayashi T, Kudo T. Steroid degradation in Comamonas testosteroni. J Steroid Biochem Mol Biol. 2012;129:4-14 pubmed publisher
    Steroid degradation by Comamonas testosteroni and Nocardia restrictus have been intensively studied for the purpose of obtaining materials for steroid drug synthesis. C...
  12. Pollack R, Thornburg L, Wu Z, Summers M. Mechanistic insights from the three-dimensional structure of 3-oxo-Delta(5)-steroid isomerase. Arch Biochem Biophys. 1999;370:9-15 pubmed
    ..In support of this hypothesis, effects on the kinetic parameters of the mutations Y14F and D99A are additive in the Y14F/D99A mutant...
  13. Maser E, Xiong G, Grimm C, Ficner R, Reuter K. 3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni: biological significance, three-dimensional structure and gene regulation. Chem Biol Interact. 2001;130-132:707-22 pubmed
    ..and is postulated to initiate the complete mineralisation of the steroid nucleus to CO(2) and H(2)O in Comamonas testosteroni. The enzyme was found to be functional towards a variety of steroid substrates, including the steroid ..
  14. Stevens J, Rao Saroja N, Jaouen M, Belghazi M, Schmitter J, Mansuy D, et al. Chaperone-assisted expression, purification, and characterization of recombinant nitrile hydratase NI1 from Comamonas testosteroni. Protein Expr Purif. 2003;29:70-6 pubmed
    ..The purified recombinant enzyme was found to be highly similar to the enzyme purified from Comamonas testosteroni according to its spectroscopic features, catalytic properties with various substrates, and post-..
  15. Teramoto M, Harayama S, Watanabe K. PhcS represses gratuitous expression of phenol-metabolizing enzymes in Comamonas testosteroni R5. J Bacteriol. 2001;183:4227-34 pubmed
    ..the transcriptional activator gene (phcR) for the expression of multicomponent phenol hydroxylase (mPH) in Comamonas testosteroni R5. The deduced product of phcS was homologous to AphS of C...
  16. Chang Y, Chuang L, Hwang C. Mechanism of proton transfer in the 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni. J Biol Chem. 2007;282:34306-14 pubmed
    3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni catalyzes the oxidation of androsterone with NAD(+) to form androstanedione and NADH with a concomitant releasing of protons to bulk solvent...
  17. Providenti M, O Brien J, Ruff J, Cook A, Lambert I. Metabolism of isovanillate, vanillate, and veratrate by Comamonas testosteroni strain BR6020. J Bacteriol. 2006;188:3862-9 pubmed
    In Comamonas testosteroni strain BR6020, metabolism of isovanillate (iVan; 3-hydroxy-4-methoxybenzoate), vanillate (Van; 4-hydroxy-3-methoxybenzoate), and veratrate (Ver; 3,4-dimethoxybenzoate) proceeds via protocatechuate (Pca; 3,4-..
  18. Monferrer D, Tralau T, Kertesz M, Dix I, Sola M, Uson I. Structural studies on the full-length LysR-type regulator TsaR from Comamonas testosteroni T-2 reveal a novel open conformation of the tetrameric LTTR fold. Mol Microbiol. 2010;75:1199-214 pubmed publisher
    ..The full-length structures of the LTTR TsaR from Comamonas testosteroni T-2 and its complex with the natural inducer para-toluensulfonate have been characterized by X-ray ..
  19. Arai H, Yamamoto T, Ohishi T, Shimizu T, Nakata T, Kudo T. Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441. Microbiology. 1999;145 ( Pt 10):2813-20 pubmed
    b>Comamonas testosteroni TA441 degrades 3-(3-hydroxyphenyl)propionate (3HPP) via the meta pathway. A gene cluster required for degradation of 3HPP was cloned from strain TA441 and sequenced...
  20. Ma Y, Zhang Y, Zhang J, Chen D, Zhu Y, Zheng H, et al. The complete genome of Comamonas testosteroni reveals its genetic adaptations to changing environments. Appl Environ Microbiol. 2009;75:6812-9 pubmed publisher
    ..Here we report the complete genome of Comamonas testosteroni strain CNB-2. Strain CNB-2 has a circular chromosome that is 5,373,643 bp long and has a G+C content of 61...
  21. Horinouchi M, Kurita T, Yamamoto T, Hatori E, Hayashi T, Kudo T. Steroid degradation gene cluster of Comamonas testosteroni consisting of 18 putative genes from meta-cleavage enzyme gene tesB to regulator gene tesR. Biochem Biophys Res Commun. 2004;324:597-604 pubmed
    Steroid degradation genes of Comamonas testosteroni TA441 are encoded in at least two gene clusters: one containing the meta-cleavage enzyme gene tesB and ORF1, 2, 3; and another consisting of ORF18, 17, tesI, H, A2, and tesA1, D, E, F, G ..
  22. Rao S, Holz R. Analyzing the catalytic mechanism of the Fe-type nitrile hydratase from Comamonas testosteroni Ni1. Biochemistry. 2008;47:12057-64 pubmed publisher
    ..k cat, K m, and k cat/ K m along with the solvent isotope effect were examined for the Fe-type NHase from Comamonas testosteroni Ni1 ( CtNHase)...
  23. Narayan K, Pandey S, Das S. Characterization of Comamonas thiooxidans sp. nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti. Curr Microbiol. 2010;61:248-53 pubmed publisher
    ..DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA ..
  24. Barriault D, Simard C, Chatel H, Sylvestre M. Characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356. Can J Microbiol. 2001;47:1025-32 pubmed
    ..Data encourage an enzyme engineering approach, consisting of introducing extensive modifications of the C-terminal portion of LB400 bphA to extend BPDO catalytic properties toward polychlorinated biphenyls...
  25. Sondossi M, Barriault D, Sylvestre M. Metabolism of 2,2'- and 3,3'-dihydroxybiphenyl by the biphenyl catabolic pathway of Comamonas testosteroni B-356. Appl Environ Microbiol. 2004;70:174-81 pubmed
    The purpose of this investigation was to examine the capacity of the biphenyl catabolic enzymes of Comamonas testosteroni B-356 to metabolize dihydroxybiphenyls symmetrically substituted on both rings...
  26. Horinouchi M, Yamamoto T, Taguchi K, Arai H, Kudo T. Meta-cleavage enzyme gene tesB is necessary for testosterone degradation in Comamonas testosteroni TA441. Microbiology. 2001;147:3367-75 pubmed
    b>Comamonas testosteroni metabolizes testosterone as the sole carbon source via a meta-cleavage reaction. A meta-cleavage enzyme gene, tesB, was cloned from C. testosteroni TA441...
  27. Providenti M, Mampel J, MacSween S, Cook A, Wyndham R. Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate. Microbiology. 2001;147:2157-67 pubmed
    A key intermediate for biodegradation of various distinct aromatic growth substrates in Comamonas testosteroni is protocatechuate (Pca), which is metabolized by the 4,5-extradiol (meta) ring fission pathway...
  28. Gul M, Ciragil P, Bulbuloglu E, Aral M, Alkis S, Ezberci F. Comamonas testosteroni bacteremia in a patient with perforated acute appendicitis. Short communication. Acta Microbiol Immunol Hung. 2007;54:317-21 pubmed
    b>Comamonas testosteroni is an uncommon isolate in the clinical laboratory as a human pathogen. C. testosteroni most commonly emerges in abdominal pathologies especially in perforated appendicitis...
  29. Xiong J, Li D, Li H, He M, Miller S, Yu L, et al. Genome analysis and characterization of zinc efflux systems of a highly zinc-resistant bacterium, Comamonas testosteroni S44. Res Microbiol. 2011;162:671-9 pubmed publisher
    A novel and multiple metal(loid)-resistant strain Comamonas testosteroni S44 with a high Zn(2+) resistance level (10 mM) was isolated...
  30. Bathe S, Schwarzenbeck N, Hausner M. Plasmid-mediated bioaugmentation of activated sludge bacteria in a sequencing batch moving bed reactor using pNB2. Lett Appl Microbiol. 2005;41:242-7 pubmed
    ..The applicability of plasmid pNB2 for bioaugmentation of bacteria in model wastewater treatment reactors receiving 3-chloroaniline (3-CA) was investigated...
  31. Baig M, Manickam N. Homology modeling and docking studies of Comamonas testosteroni B-356 biphenyl-2,3-dioxygenase involved in degradation of polychlorinated biphenyls. Int J Biol Macromol. 2010;46:47-53 pubmed publisher
    ..In the present study, the three-dimensional model of alpha-subunit of biphenyl dioxygenase (BphA) from Comamonas testosteroni B-356 has been constructed...
  32. Teramoto M, Futamata H, Harayama S, Watanabe K. Characterization of a high-affinity phenol hydroxylase from Comamonas testosteroni R5 by gene cloning, and expression in Pseudomonas aeruginosa PAO1c. Mol Gen Genet. 1999;262:552-8 pubmed
    b>Comamonas testosteroni strain R5 is a phenol-degrading bacterium which expresses a phenol-oxygenating activity that is characterized by low Ks (the apparent half-saturation constant in Haldane's equation) and low K(SI) (the apparent ..
  33. Petrillo K, Wu S, Hann E, Cooling F, Ben Bassat A, Gavagan J, et al. Over-expression in Escherichia coli of a thermally stable and regio-selective nitrile hydratase from Comamonas testosteroni 5-MGAM-4D. Appl Microbiol Biotechnol. 2005;67:664-70 pubmed
    The genes encoding a thermally stable and regio-selective nitrile hydratase (NHase) and an amidase from Comamonas testosteroni 5-MGAM-4D have been cloned and sequenced, and active NHase has been over-produced in Escherichia coli...
  34. Skowasch D, Möbus E, Maser E. Identification of a novel Comamonas testosteroni gene encoding a steroid-inducible extradiol dioxygenase. Biochem Biophys Res Commun. 2002;294:560-6 pubmed
    b>Comamonas testosteroni is a Gram-negative bacterium that can grow on steroids and polycyclic aromatic hydrocarbons (PAH) as sole carbon and energy source...
  35. Cooper G, Staples E, Iczkowski K, Clancy C. Comamonas (Pseudomonas) testosteroni endocarditis. Cardiovasc Pathol. 2005;14:145-9 pubmed
    b>Comamonas testosteroni has rarely been implicated as a human pathogen. We report a case of infectious endocarditis due to this organism, occurring in a 49-year-old man...
  36. Wu J, Chen K, Chen C, Hwang S. Hydrodynamic characteristics of immobilized cell beads in a liquid-solid fluidized-bed bioreactor. Biotechnol Bioeng. 2003;83:583-94 pubmed
    ..5. The prediction equations in this study can be further applied to analyzing the hydrodynamic characteristics of a fluidized-bed reactor using similar entrapped elastic particles as carriers. ..
  37. Boon N, De Gelder L, Lievens H, Siciliano S, Top E, Verstraete W. Bioaugmenting bioreactors for the continuous removal of 3-chloroaniline by a slow release approach. Environ Sci Technol. 2002;36:4698-704 pubmed
    ..A 3-CA metabolizing bacterium, Comamonas testosteroni strain I2, was mixed with molten agar and encapsulated in 4 mm diameter open-ended silicone tubes of 3 cm ..
  38. Oubrie A, Huizinga E, Rozeboom H, Kalk K, de Jong G, Duine J, et al. Crystallization of quinohaemoprotein alcohol dehydrogenase from Comamonas testosteroni: crystals with unique optical properties. Acta Crystallogr D Biol Crystallogr. 2001;57:1732-4 pubmed
    Quinohaemoprotein alcohol dehydrogenase from Comamonas testosteroni is a functional electron-transfer protein containing both a haem c and a pyrroloquinoline quinone cofactor...
  39. Tobajas M, Monsalvo V, Mohedano A, Rodriguez J. Enhancement of cometabolic biodegradation of 4-chlorophenol induced with phenol and glucose as carbon sources by Comamonas testosteroni. J Environ Manage. 2012;95 Suppl:S116-21 pubmed publisher
    The biological degradation of phenol and 4-chlorophenol (4-CP) by Comamonas testosteroni CECT 326T has been studied. Phenol and 4-CP were treated alone as a sole carbon and energy source, but only phenol was completely degraded by C...
  40. Chippendale T, Spanel P, Smith D. Time-resolved selected ion flow tube mass spectrometric quantification of the volatile compounds generated by E. coli JM109 cultured in two different media. Rapid Commun Mass Spectrom. 2011;25:2163-72 pubmed publisher
    ..The results of this preliminary study suggest that monitoring volatile compounds might assist in the early detection of bacterial infection in large-scale bioreactors. ..
  41. Cho H, Ha N, Choi G, Kim H, Lee D, Oh K, et al. Crystal structure of delta(5)-3-ketosteroid isomerase from Pseudomonas testosteroni in complex with equilenin settles the correct hydrogen bonding scheme for transition state stabilization. J Biol Chem. 1999;274:32863-8 pubmed
    ..Proton nuclear magnetic resonance analyses of several mutant enzymes indicate that the Tyr(14) OH forms a low barrier hydrogen bond with the dienolic oxyanion of the intermediate. ..
  42. Bathe S, Schwarzenbeck N, Hausner M. Bioaugmentation of activated sludge towards 3-chloroaniline removal with a mixed bacterial population carrying a degradative plasmid. Bioresour Technol. 2009;100:2902-9 pubmed publisher
    ..In a first step, a 3CA-degrading Comamonas testosteroni strain carrying the degradative plasmid pNB2 was added to a biofilm reactor, and complete 3CA degradation ..
  43. Yun S, Yun C, Kim S. Characterization of protocatechuate 4,5-dioxygenase induced from p-hydroxybenzoate-cultured Pseudomonas sp. K82. J Microbiol. 2004;42:152-5 pubmed
    ..These subunits were found to have more than 90% sequence homology with PmdA and PmdB of Comamonas testosteroni. The molecular weight of the native enzyme was estimated to be approximately 54 kDa, suggesting that PCD4,..
  44. Song Z, Burns R. Depolymerisation and biodegradation of a synthetic tanning agent by activated sludges, the bacteria Arthrobacter globiformis and Comamonas testosteroni, and the fungus Cunninghamella polymorpha. Biodegradation. 2005;16:305-18 pubmed
    ..The use of combinations of the depolymerising fungus C. polymorpha, and the monomer-degrading bacteria, Arthrobacter sp. 2AC and Comamonas sp. 4BC, have potential for wastewater treatment. ..
  45. Horinouchi M, Hayashi T, Koshino H, Kudo T. ORF18-disrupted mutant of Comamonas testosteroni TA441 accumulates significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and its derivatives after incubation with steroids. J Steroid Biochem Mol Biol. 2006;101:78-84 pubmed
    In a steroid degradation gene cluster of Comamonas testosteroni TA441 consisting of ORF18, 17 and tesIHA2A1DEFG, ORF18 was implicated in encoding a CoA-transferase by database searches, but the matching substrate was not clear...
  46. Hiraoka Y, Kimbara K. Rapid assessment of the physiological status of the polychlorinated biphenyl degrader Comamonas testosteroni TK102 by flow cytometry. Appl Environ Microbiol. 2002;68:2031-5 pubmed
    The viability of the polychlorinated biphenyl-degrading bacterium Comamonas testosteroni TK102 was assessed by flow cytometry (FCM) with the fluorogenic ester Calcein-AM (CAM) and the nucleic acid dye propidium iodide (PI)...
  47. Dercova K, Sejáková Z, Skokanová M, Barancikova G, Makovníková J. Bioremediation of soil contaminated with pentachlorophenol (PCP) using humic acids bound on zeolite. Chemosphere. 2007;66:783-90 pubmed
    ..The soils were sterilized and bioaugmented with the bacterial isolate Comamonas testosteroni CCM 7530...
  48. Horinouchi M, Kurita T, Hayashi T, Kudo T. Steroid degradation genes in Comamonas testosteroni TA441: Isolation of genes encoding a ?4(5)-isomerase and 3?- and 3?-dehydrogenases and evidence for a 100 kb steroid degradation gene hot spot. J Steroid Biochem Mol Biol. 2010;122:253-63 pubmed publisher
    In previous studies, we identified two major Comamonas testosteroni TA441 gene clusters involved in steroid degradation...
  49. Hoffmann F, Sotriffer C, Evers A, Xiong G, Maser E. Understanding oligomerization in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni: an in silico approach and evidence for an active protein. J Biotechnol. 2007;129:131-9 pubmed
    3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) from Comamonas testosteroni belongs to the short chain dehydrogenase/reductase (SDR) protein superfamily and catalyzes the oxidoreduction of a variety of steroid ..
  50. Xiong G, Martin H, Maser E. Identification and characterization of a novel translational repressor of the steroid-inducible 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase gene in Comamonas testosteroni. J Biol Chem. 2003;278:47400-7 pubmed
    b>Comamonas testosteroni 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3 alpha-HSD/CR) is a key enzyme in the degradation of steroid compounds in soil and may therefore play a significant role in the bioremediation of hormonally ..
  51. Nseir W, Khateeb J, Awawdeh M, Ghali M. Catheter-related bacteremia caused by Comamonas testosteroni in a hemodialysis patient. Hemodial Int. 2011;15:293-6 pubmed publisher
    b>Comamonas testosteroni has rarely been implicated as a human pathogen. In general, the outcome of C. testosteroni infections is favorable. We report a case of fatal bacteremia caused by C...
  52. Hoffman D, Okon J, Sandrin T. Medium composition affects the degree and pattern of cadmium inhibition of naphthalene biodegradation. Chemosphere. 2005;59:919-27 pubmed
    ..inhibition, we assessed the impact of cadmium on naphthalene biodegradation by a newly isolated strain of Comamonas testosteroni in three chemically-defined minimal salts media (MSM): Tris-buffered MSM, PIPES-buffered MSM, and Bushnell-..
  53. Hanoian P, Sigala P, Herschlag D, Hammes Schiffer S. Hydrogen bonding in the active site of ketosteroid isomerase: electronic inductive effects and hydrogen bond coupling. Biochemistry. 2010;49:10339-48 pubmed publisher
    ..These studies also provide experimentally testable predictions about the impact of mutating the distal tyrosine residues in this hydrogen bonding network on the NMR chemical shifts and electronic absorption spectra...
  54. Reddy A, Murthy S, Jalali S, Gopinathan U. Post-operative endophthalmitis due to an unusual pathogen, Comamonas testosteroni. J Med Microbiol. 2009;58:374-5 pubmed publisher
    Here, we describe the first report of post-operative endophthalmitis due to Comamonas testosteroni in an elderly diabetic patient after complicated cataract surgery. The isolate was identified by using Mini API strips...
  55. Linares M, Pruneda Paz J, Reyna L, Genti Raimondi S. Regulation of testosterone degradation in Comamonas testosteroni. J Steroid Biochem Mol Biol. 2008;112:145-50 pubmed publisher
    ..factor, TeiR (Testosterone-inducible Regulator), which positively regulates steroid degradation in Comamonas testosteroni. Herein, we demonstrate that TeiR interacts in vivo with steroid catabolic gene promoters...
  56. Lee H, Yoon Y, Jang D, Kim C, Cha H, Hong B, et al. 15N NMR relaxation studies of Y14F mutant of ketosteroid isomerase: the influence of mutation on backbone mobility. J Biochem. 2008;144:159-66 pubmed publisher
    The backbone dynamics of Y14F mutant of Delta(5)-3-ketosteroid isomerase (KSI) from Comamonas testosteroni has been studied in free enzyme and its complex with a steroid analogue, 19-nortestosterone hemisuccinate (19-NTHS), by 15N NMR ..
  57. Tralau T, Cook A, Ruff J. An additional regulator, TsaQ, is involved with TsaR in regulation of transport during the degradation of p-toluenesulfonate in Comamonas testosteroni T-2. Arch Microbiol. 2003;180:319-26 pubmed
    The degradation of p-toluenesulfonate (TSA) by Comamonas testosteroni T-2 is initiated by a transport system (TsaST) and enzymes (TsaMBCD) encoded on the tsa transposon, Tn tsa, on the TSA plasmid (pTSA)...
  58. Maser E, Möbus E, Xiong G. Functional expression, purification, and characterization of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni. Biochem Biophys Res Commun. 2000;272:622-8 pubmed
    ..and is postulated to initiate the complete mineralization of the steroid nucleus to CO(2) and H(2)O in Comamonas testosteroni. By this activity, 3alpha-HSD provides the basis for C...
  59. Francova K, Mackova M, Macek T, Sylvestre M. Ability of bacterial biphenyl dioxygenases from Burkholderia sp. LB400 and Comamonas testosteroni B-356 to catalyse oxygenation of ortho-hydroxychlorobiphenyls formed from PCBs by plants. Environ Pollut. 2004;127:41-8 pubmed
    ..LB400 and Comamonas testosteroni B-356, to metabolize ortho-substituted hydroxybiphenyls was tested...
  60. Chen Y, Liu H, Chen H. Characterization of phenol biodegradation by Comamonas testosteroni ZD4-1 and Pseudomonas aeruginosa ZD4-3. Biomed Environ Sci. 2003;16:163-72 pubmed
    ..To investigate the characteristic and biochemical mechanism about the phenol biodegradation by bacterial strains ZD 4-1 and ZD 4-3...
  61. Arda B, Aydemir S, Yamazhan T, Hassan A, Tunger A, Serter D. Comamonas testosteroni meningitis in a patient with recurrent cholesteatoma. APMIS. 2003;111:474-6 pubmed
    b>Comamonas testosteroni, a lesser-known member of the genus, has shown little apparent capacity for causing infections in humans. We here present a case of purulent meningitis due to C...
  62. Yun Y, Lee T, Nam G, Jang D, Shin S, Oh B, et al. Origin of the different pH activity profile in two homologous ketosteroid isomerases. J Biol Chem. 2003;278:28229-36 pubmed
    Two homologous Delta5-3-ketosteroid isomerases from Comamonas testosteroni (TI-WT) and Pseudomonas putida biotype B (PI-WT) exhibit different pH activity profiles. TI-WT loses activity below pH 5...
  63. Gong W, Xiong G, Maser E. Oligomerization and negative autoregulation of the LysR-type transcriptional regulator HsdR from Comamonas testosteroni. J Steroid Biochem Mol Biol. 2012;132:203-11 pubmed publisher
    quot;3?-Hydroxysteroid dehydrogenase/carbonyl reductase regulator" (HsdR) from Comamonas testosteroni (C. testosteroni) was identified as a member of the LysR-type transcriptional regulator (LTTR) family...
  64. Fujiki T, Nanatani K, Nishitani K, Yagi K, Ohnishi F, Yoneyama H, et al. Membrane topology of aspartate:alanine antiporter AspT from Comamonas testosteroni. J Biochem. 2007;141:85-91 pubmed
    We cloned the aspT gene encoding the L-aspartate:L-alanine antiporter AspTCt in Comamonas testosteroni genomic DNA. Analysis of the nucleotide sequence revealed that C...
  65. Kraut D, Sigala P, Pybus B, Liu C, Ringe D, Petsko G, et al. Testing electrostatic complementarity in enzyme catalysis: hydrogen bonding in the ketosteroid isomerase oxyanion hole. PLoS Biol. 2006;4:e99 pubmed
  66. Zhang G, Cong A, Xu G, Sun L, Yan Y, Xia T. [Isolation and identification of Comamonas testosteroni: cloning and overexpression of 3alpha-hydroxysteroid dehydrogenase in E.coli]. Beijing Da Xue Xue Bao Yi Xue Ban. 2005;37:203-6 pubmed
    To isolate and identify 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD) producing Comamonas testosteroni from soil, and to clone and overexpress 3alpha-HSD in E.coli.
  67. Gong W, Xiong G, Maser E. Cloning, expression and characterization of a novel short-chain dehydrogenase/reductase (SDRx) in Comamonas testosteroni. J Steroid Biochem Mol Biol. 2012;129:15-21 pubmed publisher
    ..b>Comamonas testosteroni ATCC11996 is a Gram-negative bacterium which can use steroids as carbon and energy source...