brucella melitensis

Summary

Summary: A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.

Top Publications

  1. Rajashekara G, Covert J, Petersen E, Eskra L, Splitter G. Genomic island 2 of Brucella melitensis is a major virulence determinant: functional analyses of genomic islands. J Bacteriol. 2008;190:6243-52 pubmed publisher
    ..Here, we report the identification of a GI that is important for the pathogenicity of Brucella melitensis. The deletion of GI-1, GI-5, or GI-6 did not affect bacterial growth in macrophages as well as their ..
  2. Rossetti C, Galindo C, Lawhon S, Garner H, Adams L. Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions. BMC Microbiol. 2009;9:81 pubmed publisher
    ..Further characterization of some genes found differentially expressed in the most invasive culture will likely bring new insights into the initial molecular interactions between Brucella and its host. ..
  3. Thavaselvam D, Kumar A, Tiwari S, Mishra M, Prakash A. Cloning and expression of the immunoreactive Brucella melitensis 28 kDa outer-membrane protein (Omp28) encoding gene and evaluation of the potential of Omp28 for clinical diagnosis of brucellosis. J Med Microbiol. 2010;59:421-8 pubmed publisher
    ..Cloning and expression of Brucella melitensis Omp28 protein, which is a group 3 antigen, was accomplished by PCR amplification and cloning of the gene in ..
  4. He Y, Reichow S, Ramamoorthy S, Ding X, Lathigra R, Craig J, et al. Brucella melitensis triggers time-dependent modulation of apoptosis and down-regulation of mitochondrion-associated gene expression in mouse macrophages. Infect Immun. 2006;74:5035-46 pubmed
    ..A1 cell line to infection with virulent Brucella melitensis strain 16M...
  5. Mirabella A, Yañez Villanueva R, Delrue R, Uzureau S, Zygmunt M, Cloeckaert A, et al. The two-component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength. Microbiology. 2012;158:2642-51 pubmed
    ..We identified ionic strength as a possible signal sensed by this TCS. Finally, this work correlates the absence of a functional PrlR/S system with the lack of hypersaline-induced aggregation in particular marine Brucella spp. ..
  6. Rossetti C, Galindo C, Garner H, Adams L. Transcriptional profile of the intracellular pathogen Brucella melitensis following HeLa cells infection. Microb Pathog. 2011;51:338-44 pubmed publisher
    ..Using cDNA microarray analysis, we characterized the transcriptional profile of the intracellular pathogen Brucella melitensis at 4 h (adaptational period) and 12 h (replicative phase) following HeLa cells infection...
  7. Hernández Castro R, Verdugo Rodríguez A, Puente J, Suárez Güemes F. The BMEI0216 gene of Brucella melitensis is required for internalization in HeLa cells. Microb Pathog. 2008;44:28-33 pubmed
    ..In this work, we assess the interruption of a BMEI0216 gene of Brucella melitensis, by using HeLa epithelial cells and murine macrophages for invasion and replication assays...
  8. Yang X, Walters N, Robison A, Trunkle T, Pascual D. Nasal immunization with recombinant Brucella melitensis bp26 and trigger factor with cholera toxin reduces B. melitensis colonization. Vaccine. 2007;25:2261-8 pubmed
    ..When immunized mice were challenged with B. melitensis 16M, bp26-plus Tf-immunized mice showed no difference in splenic weights but harbored three-fold less bacterial CFU when compared to sPBS-immunized control mice. ..
  9. Vrioni G, Pappas G, Priavali E, Gartzonika C, Levidiotou S. An eternal microbe: Brucella DNA load persists for years after clinical cure. Clin Infect Dis. 2008;46:e131-6 pubmed publisher
    ..b>Brucella melitensis DNA persists despite appropriate treatment and apparent recovery...

More Information

Publications97

  1. Motamedi H, Darabpour E, Gholipour M, Seyyed Nejad S. In vitro assay for the anti-Brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis. J Zhejiang Univ Sci B. 2010;11:506-11 pubmed publisher
    Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants...
  2. Ferooz J, Lemaire J, Delory M, De Bolle X, Letesson J. RpoE1, an extracytoplasmic function sigma factor, is a repressor of the flagellar system in Brucella melitensis. Microbiology. 2011;157:1263-8 pubmed publisher
    The genome of Brucella melitensis contains genes coding for the sigma factors RpoD, RpoN, RpoH1, RpoH2, RpoE1 and RpoE2. Previously published data show that B...
  3. Halling S, Peterson Burch B, Bricker B, Zuerner R, Qing Z, Li L, et al. Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. J Bacteriol. 2005;187:2715-26 pubmed
    ..genome sequence of Brucella abortus field isolate 9-941 and compare it to those of Brucella suis 1330 and Brucella melitensis 16 M...
  4. Copin R, De Baetselier P, Carlier Y, Letesson J, Muraille E. MyD88-dependent activation of B220-CD11b+LY-6C+ dendritic cells during Brucella melitensis infection. J Immunol. 2007;178:5182-91 pubmed
    ..In this study, an attempt to identify the main cellular components of the immune response induced by Brucella melitensis in vivo is made...
  5. Castaño M, Solera J. Chronic brucellosis and persistence of Brucella melitensis DNA. J Clin Microbiol. 2009;47:2084-9 pubmed publisher
    ..that had been diagnosed between 2 and 33 years previously were screened by Q-PCR for the presence of Brucella melitensis DNA and by serological tests and blood culture...
  6. Chaudhuri P, Prasad R, Kumar V, Gangaplara A. Recombinant OMP28 antigen-based indirect ELISA for serodiagnosis of bovine brucellosis. Mol Cell Probes. 2010;24:142-5 pubmed publisher
    ..In this study we evaluated a protein antigen, 28 kDa outer membrane protein (OMP28), of Brucella melitensis as an alternative to LPS. Recombinant OMP28 was produced in Escherichia coli system...
  7. Jiang H, Wang H, Xu L, Hu G, Ma J, Xiao P, et al. MLVA genotyping of Brucella melitensis and Brucella abortus isolates from different animal species and humans and identification of Brucella suis vaccine strain S2 from cattle in China. PLoS ONE. 2013;8:e76332 pubmed publisher
    ..b>Brucella melitensis (biovars 1 and 3) is the predominant species, associated with sporadic cases and outbreak in humans...
  8. Delory M, Hallez R, Letesson J, De Bolle X. An RpoH-like heat shock sigma factor is involved in stress response and virulence in Brucella melitensis 16M. J Bacteriol. 2006;188:7707-10 pubmed
    ..We also demonstrated that rpoH2 deletion strongly reduces the expression of two major virulence factors in B. melitensis, the type IV secretion system and the flagellum. ..
  9. Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. N Engl J Med. 2005;352:2325-36 pubmed
  10. Leonard S, Ferooz J, Haine V, Danese I, Fretin D, Tibor A, et al. FtcR is a new master regulator of the flagellar system of Brucella melitensis 16M with homologs in Rhizobiaceae. J Bacteriol. 2007;189:131-41 pubmed
    The flagellar regulon of Brucella melitensis 16M contains 31 genes clustered in three loci on the small chromosome...
  11. Zygmunt M, Hagius S, Walker J, Elzer P. Identification of Brucella melitensis 16M genes required for bacterial survival in the caprine host. Microbes Infect. 2006;8:2849-54 pubmed
    ..A library of 288 Brucella melitensis 16M tagged mini-Tn5 Km2 mutants, in 24 pools, was screened for its ability to colonize spleen, lymph nodes ..
  12. Uzureau S, Godefroid M, Deschamps C, Lemaire J, De Bolle X, Letesson J. Mutations of the quorum sensing-dependent regulator VjbR lead to drastic surface modifications in Brucella melitensis. J Bacteriol. 2007;189:6035-47 pubmed
    ..b>Brucella melitensis produces N-dodecanoyl homoserine lactones (C(12)-HSL), which down regulate the expression of flagellar ..
  13. Al Mariri A, Mahmoud N, Hammoud R. Efficacy evaluation of live Escherichia coli expression Brucella P39 protein combined with CpG oligodeoxynucleotides vaccine against Brucella melitensis 16M, in BALB/c mice. Biologicals. 2012;40:140-5 pubmed publisher
    ..BALB/c mice were immunized with live Escherichia coli expression the p39 gene of Brucella melitensis, a gene coding for the periplasmic binding protein. Mice were injected with either E...
  14. Dozot M, Boigegrain R, Delrue R, Hallez R, Ouahrani Bettache S, Danese I, et al. The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virB. Cell Microbiol. 2006;8:1791-802 pubmed
    ..The Rsh deletion mutants in Brucella suis and Brucella melitensis were characterized by altered morphology, and by reduced survival under starvation conditions and in ..
  15. Contreras Rodriguez A, Seleem M, Schurig G, Sriranganathan N, Boyle S, Lopez Merino A. Cloning, expression and characterization of immunogenic aminopeptidase N from Brucella melitensis. FEMS Immunol Med Microbiol. 2006;48:252-6 pubmed
    A 97-kDa purified aminopeptidase N (PepN) of Brucella melitensis was previously identified to be immunogenic in humans. The B. melitensis pepN gene was cloned, expressed in Escherichia coli and purified by affinity chromatography...
  16. Covert J, Mathison A, Eskra L, Banai M, Splitter G. Brucella melitensis, B. neotomae and B. ovis elicit common and distinctive macrophage defense transcriptional responses. Exp Biol Med (Maywood). 2009;234:1450-67 pubmed publisher
    ..melitensis infection but not other Brucella spp. were growth arrest (Gas2), immunoglobulin receptor (FcgammarI) and chemokine receptor (Cxcr4) genes, suggesting opposing effects on intracellular functions...
  17. Rolán H, den Hartigh A, Kahl McDonagh M, Ficht T, Adams L, Tsolis R. VirB12 is a serological marker of Brucella infection in experimental and natural hosts. Clin Vaccine Immunol. 2008;15:208-14 pubmed
    ..in 20/20 mice experimentally inoculated with Brucella abortus and 12/12 goats experimentally infected with Brucella melitensis. The potential use of VirB12 as a serological tool for the diagnosis of brucellosis was evaluated in the ..
  18. Ramírez Pfeiffer C, Díaz Aparicio E, Rodriguez Padilla C, Morales Loredo A, Alvarez Ojeda G, Gomez Flores R. Improved performance of Brucella melitensis native hapten over Brucella abortus OPS tracer on goat antibody detection by the fluorescence polarization assay. Vet Immunol Immunopathol. 2008;123:223-9 pubmed publisher
    ..1 strain of Brucella melitensis to improve FPA performance on goat brucellosis diagnosis...
  19. Wang F, Hu S, Gao Y, Qiao Z, Liu W, Bu Z. Complete genome sequences of Brucella melitensis strains M28 and M5-90, with different virulence backgrounds. J Bacteriol. 2011;193:2904-5 pubmed publisher
    b>Brucella melitensis is a Gram-negative coccobacillus bacteria belonging to the Alphaproteobacteria subclass. It is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. The B...
  20. Pei J, Ding X, Fan Y, Rice Ficht A, Ficht T. Toll-like receptors are critical for clearance of Brucella and play different roles in development of adaptive immunity following aerosol challenge in mice. Front Cell Infect Microbiol. 2012;2:115 pubmed publisher
    ..In addition, although TLR4 and TLR2 contribute little to control of spleen infection, a significant contribution to clearance of lung infection is described...
  21. Magnani D, Harms J, Durward M, Splitter G. Nondividing but metabolically active gamma-irradiated Brucella melitensis is protective against virulent B. melitensis challenge in mice. Infect Immun. 2009;77:5181-9 pubmed publisher
    ..Here, we demonstrate that inactivation of Brucella melitensis by gamma-irradiation inhibited its replication capability and yet retained live-Brucella protective ..
  22. Liang L, Tan X, Juarez S, Villaverde H, Pablo J, Nakajima Sasaki R, et al. Systems biology approach predicts antibody signature associated with Brucella melitensis infection in humans. J Proteome Res. 2011;10:4813-24 pubmed publisher
    ..Here we illustrate a systems biology approach to identify the antibody signature associated with Brucella melitensis (Bm) infection in humans and predict proteomic features of serodiagnostic antigens...
  23. Cassataro J, Estein S, Pasquevich K, Velikovsky C, de la Barrera S, Bowden R, et al. Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection. Infect Immun. 2005;73:8079-88 pubmed
    The immunogenicity and protective efficacy of the recombinant 31-kDa outer membrane protein from Brucella melitensis (rOmp31), administered with incomplete Freund's adjuvant, were evaluated in mice...
  24. Smits H, Espinosa B, Castillo R, Hall E, Guillen A, Zevaleta M, et al. MLVA genotyping of human Brucella isolates from Peru. Trans R Soc Trop Med Hyg. 2009;103:399-402 pubmed publisher
    Recent human Brucella melitensis isolates from Peru were genotyped by multiple locus variable number repeat analysis...
  25. Suraud V, Jacques I, Olivier M, Guilloteau L. Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep. Microbes Infect. 2008;10:1370-8 pubmed publisher
    The early distribution of Brucella melitensis and the immune response induced in lymphoid tissues and lymph nodes (LN) draining the upper respiratory tract were analysed in sheep...
  26. Liu W, Hu S, Qiao Z, Chen W, Liu L, Wang F, et al. Expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of Brucella melitensis M5-90: a potential antigen for differential serodiagnosis of brucellosis in sheep and goats. Biotechnol Appl Biochem. 2011;58:32-8 pubmed publisher
    ..A specific fragment of the bp26 gene of Brucella melitensis M5-90 was cloned into vector pQE32 to construct the recombinant plasmid (pQE32-rΔbp26)...
  27. Eskra L, Covert J, Glasner J, Splitter G. Differential expression of iron acquisition genes by Brucella melitensis and Brucella canis during macrophage infection. PLoS ONE. 2012;7:e31747 pubmed publisher
    ..Iron is a particularly scarce nutrient for bacteria with an intracellular lifestyle. Brucella melitensis and Brucella canis share ~99% of their genomes but differ in intracellular lifestyles...
  28. Valenza G, Kallmann B, Berend A, Mlynski R, Nockler K, Kurzai O, et al. Isolation of Brucella melitensis from a patient with hearing loss. Eur J Clin Microbiol Infect Dis. 2006;25:67-8 pubmed
  29. Yang Y, Yin J, Guo D, Lang X, Wang X. Immunization of mice with recombinant S-adenosyl-L-homocysteine hydrolase protein confers protection against Brucella melitensis infection. FEMS Immunol Med Microbiol. 2011;61:159-67 pubmed publisher
    ..Here, an immunoproteomic assay was used to identify the immunogenic soluble proteins of Brucella melitensis 16M...
  30. Hinic V, Brodard I, Thomann A, Cvetnic Z, Makaya P, Frey J, et al. Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J Microbiol Methods. 2008;75:375-8 pubmed publisher
    ..The assay has proven to be highly specific with the additional advantage of being suitable for use with both conventional and real-time PCR...
  31. Jiang H, Fan M, Chen J, Mi J, Yu R, Zhao H, et al. MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates. BMC Microbiol. 2011;11:256 pubmed publisher
    Since 1950, Brucella melitensis has been the predominant strain associated with human brucellosis in China. In this study we investigated the genotypic characteristics of B...
  32. Grillo M, Marin C, Barberan M, De Miguel M, Laroucau K, Jacques I, et al. Efficacy of bp26 and bp26/omp31 B. melitensis Rev.1 deletion mutants against Brucella ovis in rams. Vaccine. 2009;27:187-91 pubmed publisher
    b>Brucella melitensis Rev.1 is the most effective vaccine against B. ovis infection in sheep but induces antibodies interfering with B. melitensis diagnosis. Brucella BP26 and Omp31 proteins are differential diagnostic antigens...
  33. Wu Q, Pei J, Turse C, Ficht T. Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival. BMC Microbiol. 2006;6:102 pubmed
    ..melitensis genome. Conjugation was performed efficiently and rapidly in less than one generation in order to minimize the formation of siblings while assuring the highest level of genome coverage...
  34. Guo F, Zhang H, Chen C, Hu S, Wang Y, Qiao J, et al. Autophagy favors Brucella melitensis survival in infected macrophages. Cell Mol Biol Lett. 2012;17:249-57 pubmed publisher
    This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages...
  35. Maves R, Castillo R, Guillen A, Espinosa B, Meza R, Espinoza N, et al. Antimicrobial susceptibility of Brucella melitensis isolates in Peru. Antimicrob Agents Chemother. 2011;55:1279-81 pubmed publisher
    Brucellosis is an important public health problem in Peru. We evaluated 48 human Brucella melitensis biotype 1 strains from Peru between 2000 and 2006...
  36. Al Mariri A. Ultraviolet C lethal effect on Brucella melitensis. New Microbiol. 2008;31:47-55 pubmed
    The gram-negative bacteria Brucella melitensis was investigated to evaluate its susceptibility to UVC radiation at 254 nm. At an intensity of 18.7 mW/cm2 of UVC, the time required for inactivation of B...
  37. Smither S, Perkins S, Davies C, Stagg A, Nelson M, Atkins H. Development and characterization of mouse models of infection with aerosolized Brucella melitensis and Brucella suis. Clin Vaccine Immunol. 2009;16:779-83 pubmed publisher
    ..We have developed mouse models of infection with aerosolized Brucella melitensis and Brucella suis and demonstrated their utility for the evaluation of vaccines using the model live B...
  38. Chanet V, Gourdon F, Baud O, Beytout J, Romaszko J. Brucella melitensis in a married couple after a trip through Sicily. South Med J. 2005;98:843-4 pubmed
  39. Amirmozafari N, Ghazi F, Mostafazadeh A, Mostafaie A, Rajabnia R. Comparison of heat shock response in Brucella abortus and Brucella melitensis. Pak J Biol Sci. 2008;11:188-94 pubmed
    ..Therefore, hsp-60 can be a good antigenic candidate for engineering subunit vaccine against Brucella, as well as for ELISA test development...
  40. Liang L, Leng D, Burk C, Nakajima Sasaki R, Kayala M, Atluri V, et al. Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. PLoS Negl Trop Dis. 2010;4:e673 pubmed publisher
    ..These data demonstrate that an experimentally infected natural reservoir host produces a fundamentally different immune response than a naturally infected accidental human host...
  41. Nockler K, Maves R, Cepeda D, Draeger A, Mayer Scholl A, Chacaltana J, et al. Molecular epidemiology of Brucella genotypes in patients at a major hospital in central Peru. J Clin Microbiol. 2009;47:3147-55 pubmed publisher
    The multiple-locus variable-number repeat analysis of 90 human Brucella melitensis isolates from a large urban area in central Peru revealed variations at 4 (Bruce07, Bruce09, Bruce18, and Bruce42) out of 16 loci investigated, of which 1 ..
  42. Wang Y, Bai Y, Qu Q, Xu J, Chen Y, Zhong Z, et al. The 16M?vjbR as an ideal live attenuated vaccine candidate for differentiation between Brucella vaccination and infection. Vet Microbiol. 2011;151:354-62 pubmed publisher
    ..Antibodies to Brucella melitensis VjbR could be detected in sera from patients and animals with brucellosis but not in control ones, implying ..
  43. García Yoldi D, Marin C, de Miguel M, Muñoz P, Vizmanos J, López Goñi I. Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin Chem. 2006;52:779-81 pubmed
  44. Vitry M, De Trez C, Goriely S, Dumoutier L, Akira S, Ryffel B, et al. Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice. Infect Immun. 2012;80:4271-80 pubmed publisher
    ..antigen presentation as well as T helper 1 (Th1), Th2, and Th17-mediated responses on the immune control of Brucella melitensis 16 M infection...
  45. Delrue R, Deschamps C, Léonard S, Nijskens C, Danese I, Schaus J, et al. A quorum-sensing regulator controls expression of both the type IV secretion system and the flagellar apparatus of Brucella melitensis. Cell Microbiol. 2005;7:1151-61 pubmed
    Both a type IV secretion system and a flagellum have been described in Brucella melitensis. These two multimolecular surface appendages share several features...
  46. Mancilla M, Grillo M, de Miguel M, López Goñi I, San Roman B, Zabalza Baranguá A, et al. Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine. Vet Res. 2013;44:105 pubmed publisher
    b>Brucella melitensis Rev 1 is the best vaccine available for the prophylaxis of small ruminant brucellosis and, indirectly, for reducing human brucellosis...
  47. Godefroid M, Svensson M, Cambier P, Uzureau S, Mirabella A, De Bolle X, et al. Brucella melitensis 16M produces a mannan and other extracellular matrix components typical of a biofilm. FEMS Immunol Med Microbiol. 2010;59:364-77 pubmed publisher
    Mutations in the Brucella melitensis quorum-sensing (QS) system are involved in the formation of clumps containing an exopolysaccharide. Here, we show that the overexpression of a gene called aiiD in B...
  48. Laloux G, Deghelt M, de Barsy M, Letesson J, De Bolle X. Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening. PLoS ONE. 2010;5:e13274 pubmed publisher
    ..Brucellae, which are the causative agent of a worldwide zoonosis, prevent apoptosis of infected cells, probably to support survival of their replication niche...
  49. Wu H, Zuo Y, Cui C, Yang W, Ma H, Wang X. Rapid quantitative detection of Brucella melitensis by a label-free impedance immunosensor based on a gold nanoparticle-modified screen-printed carbon electrode. Sensors (Basel). 2013;13:8551-63 pubmed publisher
    A rapid and simple method for quantitative monitoring of Brucella melitensis using electrochemical impedance spectroscopy (EIS) is reported for the first time...
  50. Vitry M, Hanot Mambres D, De Trez C, Akira S, Ryffel B, Letesson J, et al. Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis. J Immunol. 2014;192:3740-52 pubmed publisher
    ..generation of a protective immune memory response following priming by the injection of heat-killed or live Brucella melitensis 16M...
  51. Lecaroz C, Blanco Prieto M, Burrell M, Gamazo C. Intracellular killing of Brucella melitensis in human macrophages with microsphere-encapsulated gentamicin. J Antimicrob Chemother. 2006;58:549-56 pubmed
    ..The aim of this work was to prepare and characterize particulate carriers containing gentamicin and to study their interactions with phagocytic cells and bactericidal activity against intracellular Brucella melitensis.
  52. Resendiz Sánchez J, Contreras Rodriguez A, Lopez Merino A, Bravo Guzmán L, Valle Valdez J. Isolation of Brucella melitensis from an abscess on the left foot of a 3-year-old infant. J Med Microbiol. 2009;58:267-9 pubmed publisher
    ..These were biochemically and molecularly identified as Brucella melitensis. The possibility of foot abscess being caused by Brucella should be considered in countries where ..
  53. Yamazhan T, Aydemir S, Tunger A, Serter D, Gökengin D. In vitro activities of various antimicrobials against Brucella melitensis strains in the Aegean region in Turkey. Med Princ Pract. 2005;14:413-6 pubmed
    To study in vitro activities of three quinolones (ciprofloxacin, levofloxacin, moxifloxacin), four macrolides (erythromycin, dirithromycin, azithromycin, clarithromycin) and doxycycline against 44 clinical isolates of Brucella melitensis.
  54. Dricot A, Rual J, Lamesch P, Bertin N, Dupuy D, Hao T, et al. Generation of the Brucella melitensis ORFeome version 1.1. Genome Res. 2004;14:2201-6 pubmed
    ..Using the complete genome sequence of Brucella melitensis, we generated a database of protein-coding open reading frames (ORFs) and constructed an ORFeome library of ..
  55. Wang F, Hu S, Liu W, Qiao Z, Gao Y, Bu Z. Deep-sequencing analysis of the mouse transcriptome response to infection with Brucella melitensis strains of differing virulence. PLoS ONE. 2011;6:e28485 pubmed publisher
    b>Brucella melitensis is an important zoonotic pathogen that causes brucellosis, a disease that affects sheep, cattle and occasionally humans. B. melitensis strain M5-90, a live attenuated vaccine cultured from B...
  56. Chaudhary A, Ganguly K, Cabantous S, Waldo G, Micheva Viteva S, Nag K, et al. The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun. 2012;417:299-304 pubmed publisher
    The pathogen Brucella melitensis secretes a Toll/interleukin-1 receptor (TIR) domain containing protein that abrogates host innate immune responses...
  57. Rajashekara G, Glover D, Banai M, O Callaghan D, Splitter G. Attenuated bioluminescent Brucella melitensis mutants GR019 (virB4), GR024 (galE), and GR026 (BMEI1090-BMEI1091) confer protection in mice. Infect Immun. 2006;74:2925-36 pubmed
    ..Previously, we have applied bioluminescence imaging to gain greater insight into Brucella melitensis pathogenesis...
  58. Marianelli C, Petrucca A, Pasquali P, Ciuchini F, Papadopoulou S, Cipriani P. Use of MLVA-16 typing to trace the source of a laboratory-acquired Brucella infection. J Hosp Infect. 2008;68:274-6 pubmed publisher
  59. Billard E, Cazevieille C, Dornand J, Gross A. High susceptibility of human dendritic cells to invasion by the intracellular pathogens Brucella suis, B. abortus, and B. melitensis. Infect Immun. 2005;73:8418-24 pubmed
    ..Here we report that DCs display a great susceptibility to Brucella infection. Therefore, DCs might serve as a reservoir and be important for the development of Brucella bacteria within their host...
  60. Lázaro F, Rodríguez Tarazona R, Garcia Rodriguez J, Muñoz Bellido J. Fluoroquinolone-resistant Brucella melitensis mutants obtained in vitro. Int J Antimicrob Agents. 2009;34:252-4 pubmed publisher
    Three fluoroquinolone-resistant Brucella melitensis mutants were obtained after successive passages on norfloxacin-containing agar plates. The original strain was B. melitensis biovar 2 ATCC 23457...
  61. Aliskan H, Turunc T, Demiroglu Y, Colakoglu S, Arslan H. [Short communication: Investigation of in vitro antibiotic susceptibility of Brucella melitensis]. Mikrobiyol Bul. 2008;42:125-9 pubmed
    The aim of this study was to investigate the in vitro susceptibilities of Brucella melitensis clinical isolates against antibiotics used in conventional treatment regimens and additionally against quinolones. A total of 65 B...
  62. Ferreira A, Chambel L, Tenreiro T, Cardoso R, Flor L, Dias I, et al. MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates. PLoS ONE. 2012;7:e42514 pubmed publisher
    ..among isolates, the MLVA16(Orsay) assay (panels 1, 2A and 2B) was performed with a collection of 126 Brucella melitensis (46 human and 80 animal isolates) and 157 B...
  63. Mantecón M, Gutiérrez P, del Pilar Zarzosa M, Dueñas A, Solera J, Fernández Lago L, et al. Utility of an immunocapture-agglutination test and an enzyme-linked immunosorbent assay test against cytosolic proteins from Brucella melitensis B115 in the diagnosis and follow-up of human acute brucellosis. Diagn Microbiol Infect Dis. 2006;55:27-35 pubmed
    ..immunosorbent assay IgG, IgA, and IgM (ELISA-IgG, ELISA-IgA, ELISA-IgM) against cytosolic proteins from Brucella melitensis B115 (R) was compared with ELISA-IgG, ELISA-IgA, and ELISA-IgM against smooth lipopolysaccharide (S-LPS) ..
  64. Kato Y, Masuda G, Itoda I, Imamura A, Ajisawa A, Negishi M. Brucellosis in a returned traveler and his wife: probable person-to-person transmission of Brucella melitensis. J Travel Med. 2007;14:343-5 pubmed
  65. Orhan G, Bayram A, Zer Y, Balci I. Synergy tests by E test and checkerboard methods of antimicrobial combinations against Brucella melitensis. J Clin Microbiol. 2005;43:140-3 pubmed
    ..test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 Brucella melitensis strains isolated from blood cultures...
  66. Celebi G, Kulah C, Kilic S, Ustundag G. Asymptomatic Brucella bacteraemia and isolation of Brucella melitensis biovar 3 from human breast milk. Scand J Infect Dis. 2007;39:205-8 pubmed
    ..Two of them (mother and sister) were asymptomatic and had no clinical findings. Brucella melitensis biovar 3 was isolated from breast milk culture and from all blood cultures of 4 brucellosis cases...
  67. Caliskan A, Barut S, Koseoglu D, Aytan H, Demirturk F. [Obturatory abscess and pelvic pain caused by Brucella melitensis]. Mikrobiyol Bul. 2009;43:325-9 pubmed
    ..b>Brucella melitensis was isolated from the capsule of pelvic mass on the fifth day of culture in BacT/ALERT Selectlink (Organon ..
  68. Alvarez J, Sáez J, Garcia N, Serrat C, Pérez Sancho M, Gonzalez S, et al. Management of an outbreak of brucellosis due to B. melitensis in dairy cattle in Spain. Res Vet Sci. 2011;90:208-11 pubmed publisher
    b>Brucella melitensis is a major human and animal pathogen, with a wide host range that includes all domestic ruminant species, although small ruminants are its preferred hosts. Outbreaks in cattle due to B...
  69. El Tras W, Tayel A, Eltholth M, Guitian J. Brucella infection in fresh water fish: Evidence for natural infection of Nile catfish, Clarias gariepinus, with Brucella melitensis. Vet Microbiol. 2010;141:321-5 pubmed publisher
    ..The public should be aware of the consequences of disposing of animal waste into the canals and public health authorities should consider the potential role of catfish as a source of infection...
  70. Yang Y, Wang L, Yin J, Wang X, Cheng S, Lang X, et al. Immunoproteomic analysis of Brucella melitensis and identification of a new immunogenic candidate protein for the development of brucellosis subunit vaccine. Mol Immunol. 2011;49:175-84 pubmed publisher
    ..of a brucellosis subunit vaccine, an immunoproteomic assay was used to identify immunogenic proteins from Brucella melitensis 16 M soluble proteins...
  71. Jennings G, Hajjeh R, Girgis F, Fadeel M, Maksoud M, Wasfy M, et al. Brucellosis as a cause of acute febrile illness in Egypt. Trans R Soc Trop Med Hyg. 2007;101:707-13 pubmed
    ..The high incidence of brucellosis in Fayoum highlights its public health importance, and the need to implement prevention strategies in humans and animals...
  72. Kahl McDonagh M, Arenas Gamboa A, Ficht T. Aerosol infection of BALB/c mice with Brucella melitensis and Brucella abortus and protective efficacy against aerosol challenge. Infect Immun. 2007;75:4923-32 pubmed
  73. Fretin D, Fauconnier A, K hler S, Halling S, L onard S, Nijskens C, et al. The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection. Cell Microbiol. 2005;7:687-98 pubmed publisher
    ..We demonstrated that in the early log phase of a growth curve in 2YT nutrient broth, Brucella melitensis expresses genes corresponding to the basal (MS ring) and the distal (hook and filament) parts of the ..
  74. Cassataro J, Velikovsky C, de la Barrera S, Estein S, Bruno L, Bowden R, et al. A DNA vaccine coding for the Brucella outer membrane protein 31 confers protection against B. melitensis and B. ovis infection by eliciting a specific cytotoxic response. Infect Immun. 2005;73:6537-46 pubmed
    ..The protective response could be related to the induction of CD8(+) T cells that eliminate Brucella-infected cells via the perforin pathway...
  75. Weeks J, Galindo C, DRAKE K, Adams G, Garner H, Ficht T. Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression. BMC Microbiol. 2010;10:167 pubmed publisher
    ..Deletion of the luxR homologue vjbR highly attenuates intracellular survival of Brucella melitensis and has been interpreted to be an indication of a role for QS in Brucella infection...
  76. Arenas Gamboa A, Rice Ficht A, Kahl McDonagh M, Ficht T. Protective efficacy and safety of Brucella melitensis 16M?mucR against intraperitoneal and aerosol challenge in BALB/c mice. Infect Immun. 2011;79:3653-8 pubmed publisher
    ..These results suggest that the 16M?mucR vaccine is safe, elicits a strong protective immunity, and should be considered as a promising vaccine candidate for human use...
  77. Swartz T, Tseng T, Frederickson M, Paris G, Comerci D, Rajashekara G, et al. Blue-light-activated histidine kinases: two-component sensors in bacteria. Science. 2007;317:1090-3 pubmed publisher
    ..We have discovered that the prokaryotes Brucella melitensis, Brucella abortus, Erythrobacter litoralis, and Pseudomonas syringae contain light-activated histidine ..
  78. Ramírez Pfeiffer C, Díaz Aparicio E, Gomez Flores R, Rodriguez Padilla C, Morales Loredo A, Alvarez Ojeda G. Use of the Brucella melitensis native hapten to diagnose brucellosis in goats by a rapid, simple, and specific fluorescence polarization assay. Clin Vaccine Immunol. 2008;15:911-5 pubmed publisher
    The performance of the fluorescence polarization assay (FPA) using the recently described Brucella melitensis native hapten and the Brucella abortus O-polysaccharide tracer was evaluated and compared with those of The World Organization ..
  79. Kilic S, Dizbay M, Cabadak H. In vitro activity of tigecycline, tetracycline and fluoroquinolones against Brucella melitensis. J Chemother. 2008;20:33-7 pubmed
    ..The aim of this study was to assess the in vitro susceptibility of 60 Brucella melitensis isolates obtained from blood and fluids to tigecycline, tetracycline and three quinolones (ciprofloxacin, ..
  80. Gonzalez D, Grillo M, de Miguel M, Ali T, Arce Gorvel V, Delrue R, et al. Brucellosis vaccines: assessment of Brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and export. PLoS ONE. 2008;3:e2760 pubmed publisher
    ..In endemic areas, vaccination is the only effective way to control this disease. Brucella melitensis Rev 1 is a vaccine effective against the brucellosis of sheep and goat caused by B...
  81. Kattar M, Jaafar R, Araj G, Le Fleche P, Matar G, Abi Rached R, et al. Evaluation of a multilocus variable-number tandem-repeat analysis scheme for typing human Brucella isolates in a region of brucellosis endemicity. J Clin Microbiol. 2008;46:3935-40 pubmed publisher
    ..All isolates were identified as Brucella melitensis by MLVA-16 and were found to be closely related to B...
  82. Radhakrishnan G, Splitter G. Biochemical and functional analysis of TIR domain containing protein from Brucella melitensis. Biochem Biophys Res Commun. 2010;397:59-63 pubmed publisher
    ..With the objective of detailed characterization, we have over expressed and purified TcpB from Brucella melitensis in native condition. The purified protein exhibited lipid-binding properties and cell permeability...
  83. Barrio M, Grilló M, Muñoz P, Jacques I, Gonzalez D, de Miguel M, et al. Rough mutants defective in core and O-polysaccharide synthesis and export induce antibodies reacting in an indirect ELISA with smooth lipopolysaccharide and are less effective than Rev 1 vaccine against Brucella melitensis infection of sheep. Vaccine. 2009;27:1741-9 pubmed publisher
    ..Here, Brucella melitensis R mutants in all main lipopolysaccharide biosynthetic pathways were evaluated in sheep in comparison with ..
  84. Zhang X, Ren J, Li N, Liu W, Wu Q. Disruption of the BMEI0066 gene attenuates the virulence of Brucella melitensis and decreases its stress tolerance. Int J Biol Sci. 2009;5:570-7 pubmed
    b>Brucella melitensis is a facultative intracellular pathogen...
  85. Grillo M, De Miguel M, Muñoz P, Marin C, Ariza J, Blasco J. Efficacy of several antibiotic combinations against Brucella melitensis Rev 1 experimental infection in BALB/c mice. J Antimicrob Chemother. 2006;58:622-6 pubmed
    ..of the present study was to compare the efficacy of gentamicin given alone or combined with doxycycline with that of standard combination therapies in BALB/c mice experimentally infected with the Brucella melitensis vaccine strain Rev 1.
  86. Kumar A, Tiwari S, Thavaselvam D, Sathyaseelan K, Prakash A, Barua A, et al. Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and ?-mercaptoethanol. Protein Expr Purif. 2012;83:226-32 pubmed publisher
    ..In this study recombinant Omp28 (rOmp28) protein of Brucella melitensis was expressed in two different vector systems and further efficient purification of the protein was done by ..
  87. Haine V, Dozot M, Dornand J, Letesson J, De Bolle X. NnrA is required for full virulence and regulates several Brucella melitensis denitrification genes. J Bacteriol. 2006;188:1615-9 pubmed
    We identified two regulators of denitrification genes in Brucella melitensis 16M: NarR, which regulates the nitrate reductase (nar) operon, and NnrA, which is involved in the expression of the last three reductases of the denitrification ..
  88. Ozturk O, Akcam Z, Sahin U, Bircan A, Akkaya A, Yayli G. [A rare agent of pneumonia: Brucella melitensis]. Tuberk Toraks. 2008;56:443-7 pubmed
    ..Herein, we report a pneumonia case which proved due to Brucella melitensis in a 57-year-old man who presented with chronic cough, sputum, hemoptysis and fever.