brucella abortus

Summary

Summary: A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.

Top Publications

  1. Xavier M, Winter M, Spees A, den Hartigh A, Nguyen K, Roux C, et al. PPAR?-mediated increase in glucose availability sustains chronic Brucella abortus infection in alternatively activated macrophages. Cell Host Microbe. 2013;14:159-70 pubmed publisher
    ..Here we show that the intracellular pathogen Brucella abortus survives and replicates preferentially in alternatively activated macrophages (AAMs), which are more abundant ..
  2. Bukata L, Altabe S, de Mendoza D, Ugalde R, Comerci D. Phosphatidylethanolamine synthesis is required for optimal virulence of Brucella abortus. J Bacteriol. 2008;190:8197-203 pubmed publisher
    ..These results suggest that membrane phospholipid composition is critical for the interaction of B. abortus with the host cell. ..
  3. García Samartino C, Delpino M, Pott Godoy C, Di Genaro M, Pasquevich K, Zwerdling A, et al. Brucella abortus induces the secretion of proinflammatory mediators from glial cells leading to astrocyte apoptosis. Am J Pathol. 2010;176:1323-38 pubmed publisher
    ..Our results provide proof of the principle that Brucella lipoproteins could be key virulence factors in neurobrucellosis and that astrogliosis might contribute to neurobrucellosis pathogenesis. ..
  4. Oliveira S, de Oliveira F, Macedo G, de Almeida L, Carvalho N. The role of innate immune receptors in the control of Brucella abortus infection: toll-like receptors and beyond. Microbes Infect. 2008;10:1005-9 pubmed publisher
    ..The better understanding of the role by such innate immune receptors in bacterial infection is critical in host-pathogen interactions. ..
  5. Rolán H, Tsolis R. Mice lacking components of adaptive immunity show increased Brucella abortus virB mutant colonization. Infect Immun. 2007;75:2965-73 pubmed
    The Brucella abortus type IV secretion system (T4SS), encoded by the virB genes, is essential for survival in mononuclear phagocytes in vitro. In the mouse model, a B...
  6. Nielsen K, Smith P, Yu W, Elmgren C, Nicoletti P, Perez B, et al. Second generation competitive enzyme immunoassay for detection of bovine antibody to Brucella abortus. Vet Microbiol. 2007;124:173-7 pubmed
    A second generation competitive enzyme immunoassay (CELISA) for detection of bovine antibody to Brucella abortus was developed...
  7. Conde Alvarez R, Arce Gorvel V, Iriarte M, Mancek Keber M, Barquero Calvo E, Palacios Chaves L, et al. The lipopolysaccharide core of Brucella abortus acts as a shield against innate immunity recognition. PLoS Pathog. 2012;8:e1002675 pubmed publisher
    ..These results also encourage for an improvement in the generation of novel bacterial vaccines...
  8. Macedo A, Costa E, Silva A, Paixão T, Santos R. Monocyte-derived macrophages from Zebu (Bos taurus indicus) are more efficient to control Brucella abortus intracellular survival than macrophages from European cattle (Bos taurus taurus). Vet Immunol Immunopathol. 2013;151:294-302 pubmed publisher
    ..in cattle, and it is associated with the ability of macrophages to prevent intracellular replication of Brucella abortus. Identification of breeds that are resistant to B...
  9. Bankole A, Saegerman C, Berkvens D, Fretin D, Geerts S, Ieven G, et al. Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia. Vet Rec. 2010;166:753-6 pubmed publisher
    ..Three isolates of Brucella, biotyped as Brucella abortus biovar 3, were cultured from six hygroma fluid samples...

More Information

Publications100

  1. Céspedes S, Salgado P, Valenzuela P, Vidal R, Oñate A. Characterization of genomic island 3 and genetic variability of Chilean field strains of Brucella abortus. J Clin Microbiol. 2011;49:2461-9 pubmed publisher
    ..In relation to the study of GI-3, our experimental analysis by TP-PCR identified and confirmed that GI-3 is present in all wild strains of B. abortus, demonstrating the high stability of gene cluster GI-3 in Chilean field strains. ..
  2. Caswell C, Elhassanny A, Planchin E, Roux C, Weeks Gorospe J, Ficht T, et al. Diverse genetic regulon of the virulence-associated transcriptional regulator MucR in Brucella abortus 2308. Infect Immun. 2013;81:1040-51 pubmed publisher
    ..Here, we show that a Brucella abortus in-frame mucR deletion strain exhibits a pronounced growth defect during in vitro cultivation and, more ..
  3. Yu D, Hu X, Cai H. A combined DNA vaccine encoding BCSP31, SOD, and L7/L12 confers high protection against Brucella abortus 2308 by inducing specific CTL responses. DNA Cell Biol. 2007;26:435-43 pubmed
    ..Immunization of mice with the combined DNA vaccine offered high protection against Brucella abortus (B. abortus) infection...
  4. Cadmus S, Adesokan H, Ajala O, Odetokun W, Perrett L, Stack J. Seroprevalence of Brucella abortus and B. canis in household dogs in southwestern Nigeria: a preliminary report. J S Afr Vet Assoc. 2011;82:56-7 pubmed
    ..dogs in Lagos and Ibadan, southwestern Nigeria, was carried out to determine antibodies due to exposure to Brucella abortus and B. canis, using the rose bengal test (RBT) and the rapid slide agglutination (RSA) test, respectively...
  5. Zhang M, Han X, Liu H, Tian M, Ding C, Song J, et al. Inactivation of the ABC transporter ATPase gene in Brucella abortus strain 2308 attenuated the virulence of the bacteria. Vet Microbiol. 2013;164:322-9 pubmed publisher
    b>Brucella abortus is a Gram-negative, facultative intracellular bacterial pathogen of human and other animals. Brucella lipopolysaccharide has been identified as an important virulence factor...
  6. Gardenghi S, Renaud T, Meloni A, Casu C, Crielaard B, Bystrom L, et al. Distinct roles for hepcidin and interleukin-6 in the recovery from anemia in mice injected with heat-killed Brucella abortus. Blood. 2014;123:1137-45 pubmed publisher
    ..AI was induced with heat-killed Brucella abortus (BA)...
  7. Kaushik P, Singh D, Kumar S, Tiwari A, Shukla G, Dayal S, et al. Protection of mice against Brucella abortus 544 challenge by vaccination with recombinant OMP28 adjuvanted with CpG oligonucleotides. Vet Res Commun. 2010;34:119-32 pubmed publisher
    b>Brucella abortus, a gram negative, facultative intracellular pathogen causes brucellosis in many animal species and humans. Although live, attenuated vaccines are available against this infection, they suffer from certain limitations...
  8. de Barsy M, Mirabella A, Letesson J, De Bolle X. A Brucella abortus cstA mutant is defective for association with endoplasmic reticulum exit sites and displays altered trafficking in HeLa cells. Microbiology. 2012;158:2610-8 pubmed
    ..We found that a tagged CstA is secreted in Brucella abortus culture medium...
  9. Tachibana M, Watanabe K, Yamasaki Y, Suzuki H, Watarai M. Expression of heme oxygenase-1 is associated with abortion caused by Brucella abortus infection in pregnant mice. Microb Pathog. 2008;45:105-9 pubmed publisher
    b>Brucella abortus is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant (TG) cells, and the causative agent of brucellosis...
  10. Manterola L, Guzmán Verri C, Chaves Olarte E, Barquero Calvo E, de Miguel M, Moriyon I, et al. BvrR/BvrS-controlled outer membrane proteins Omp3a and Omp3b are not essential for Brucella abortus virulence. Infect Immun. 2007;75:4867-74 pubmed
    The Brucella abortus two-component regulatory system BvrR/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22)...
  11. Lamontagne J, Forest A, Marazzo E, Denis F, Butler H, Michaud J, et al. Intracellular adaptation of Brucella abortus. J Proteome Res. 2009;8:1594-609 pubmed publisher
    Macrophages were infected with virulent Brucella abortus strain 2308 or attenuated strain 19. Intracellular bacteria were recovered at different times after infection and their proteomes compared...
  12. Pasquevich K, Estein S, García Samartino C, Samartino C, Zwerdling A, Coria L, et al. Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection. Infect Immun. 2009;77:436-45 pubmed publisher
    ..Overall these results indicate that U-Omp16 or U-Omp19 would be a useful candidate for a subunit vaccine against human and animal brucellosis. ..
  13. Starr T, Child R, Wehrly T, Hansen B, Hwang S, López Otin C, et al. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe. 2012;11:33-45 pubmed publisher
    ..However, pathogens have evolved mechanisms to subvert this process. The intracellular bacterium Brucella abortus ensures its survival by forming the Brucella-containing vacuole (BCV), which traffics from the endocytic ..
  14. Garofolo G, Di Giannatale E, De Massis F, Zilli K, Ancora M, Cammà C, et al. Investigating genetic diversity of Brucella abortus and Brucella melitensis in Italy with MLVA-16. Infect Genet Evol. 2013;19:59-70 pubmed publisher
    ..We conducted a genetic assessment of 206 isolates of Brucella abortus and B. melitensis from Italy using Variable Number Tandem Repeats (VNTRs)...
  15. Salcedo S, Marchesini M, Lelouard H, Fugier E, Jolly G, Balor S, et al. Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1. PLoS Pathog. 2008;4:e21 pubmed publisher
    ..These results show that intracellular Brucella is able to control dendritic cell function, which may have important consequences in the development of chronic brucellosis. ..
  16. Caswell C, Gaines J, Ciborowski P, Smith D, Borchers C, Roux C, et al. Identification of two small regulatory RNAs linked to virulence in Brucella abortus 2308. Mol Microbiol. 2012;85:345-60 pubmed publisher
    ..Two proteins encoded by BAB1_1794 and BAB2_0612 are highly over-produced in a Brucella abortus hfq mutant compared with the parental strain, and recently, expression of orthologues of these proteins in ..
  17. Adone R, Francia M, Pistoia C, Petrucci P, Pesciaroli M, Pasquali P. Protective role of antibodies induced by Brucella melitensis B115 against B. melitensis and Brucella abortus infections in mice. Vaccine. 2012;30:3992-5 pubmed publisher
    ..strain, directed to antigens other than O-PS, passively tranferred to untreated mice prior to infection with Brucella abortus 2308 and B. melitensis 16M virulent strains...
  18. Adone R, Francia M, Ciuchini F. Brucella melitensis B115-based complement fixation test to detect antibodies induced by Brucella rough strains. J Appl Microbiol. 2008;105:567-74 pubmed publisher
    ..naturally devoid of anticomplementary activity, used as antigen in a complement fixation test (CFT) to detect antibodies induced by Brucella strains with rough phenotype, such as Brucella abortus RB51, Brucella ovis and Brucella canis.
  19. Sieira R, Arocena G, Bukata L, Comerci D, Ugalde R. Metabolic control of virulence genes in Brucella abortus: HutC coordinates virB expression and the histidine utilization pathway by direct binding to both promoters. J Bacteriol. 2010;192:217-24 pubmed publisher
    ..The virB operon of Brucella abortus codes for a T4SS that is essential for virulence and intracellular multiplication of the bacterium in the ..
  20. Capparelli R, Borriello G, Marabelli R, Roperto S, Roperto F, Iannelli D. The Nramp1AA genotype confers susceptibility to Brucella abortus in water buffalo. Mamm Genome. 2007;18:137-43 pubmed
    ..The Nramp1BB genotype is associated with resistance of water buffalo to the intracellular pathogen Brucella abortus. This article provides evidence that the Nramp1AA genotype is associated with susceptibility to the same ..
  21. Sabio y García J, Bigi F, Rossetti O, Campos E. Expression of MPB83 from Mycobacterium bovis in Brucella abortus S19 induces specific cellular immune response against the recombinant antigen in BALB/c mice. Microbes Infect. 2010;12:1236-43 pubmed publisher
    ..to either ?-galactosidase, outer membrane lipoprotein OMP19 or periplasmic protein BP26 in gram-negative Brucella abortus S19, in all cases driven by each gene's own promoter...
  22. Scian R, Barrionuevo P, Fossati C, Giambartolomei G, Delpino M. Brucella abortus invasion of osteoblasts inhibits bone formation. Infect Immun. 2012;80:2333-45 pubmed publisher
    ..In this article, we describe immune mechanisms whereby Brucella abortus may invade and replicate within osteoblasts, inducing apoptosis, inhibiting mineral and organic matrix ..
  23. Capparelli R, Parlato M, Iannaccone M, Roperto S, Marabelli R, Roperto F, et al. Heterogeneous shedding of Brucella abortus in milk and its effect on the control of animal brucellosis. J Appl Microbiol. 2009;106:2041-7 pubmed publisher
    To ascertain whether in Brucella abortus-infected water buffalo herds, the number of newly infected animals could be reduced by culling superspreaders (the animals secreting > or =10(4) CFU per ml of milk). The number of B...
  24. Higgins J, Stuber T, Quance C, Edwards W, Tiller R, Linfield T, et al. Molecular epidemiology of Brucella abortus isolates from cattle, elk, and bison in the United States, 1998 to 2011. Appl Environ Microbiol. 2012;78:3674-84 pubmed publisher
    A variable-number tandem repeat (VNTR) protocol targeting 10 loci in the Brucella abortus genome was used to assess genetic diversity among 366 field isolates recovered from cattle, bison, and elk in the Greater Yellowstone Area (GYA) and ..
  25. Bayemi P, Webb E, Nsongka M, Unger H, Njakoi H. Prevalence of Brucella abortus antibodies in serum of Holstein cattle in Cameroon. Trop Anim Health Prod. 2009;41:141-4 pubmed publisher
    Holstein cattle of a small scale dairy production systems were screened for Brucella abortus antibodies in 21 villages in Cameroon by ELISA. Results show a general seroprevalence of 8.4% in Holstein cattle...
  26. Makita K, Fèvre E, Waiswa C, Eisler M, Welburn S. How human brucellosis incidence in urban Kampala can be reduced most efficiently? A stochastic risk assessment of informally-marketed milk. PLoS ONE. 2010;5:e14188 pubmed publisher
    ..of human brucellosis infection through consumption of informally marketed raw milk potentially infected with Brucella abortus in Kampala and to identify the best control options...
  27. Chen F, He Y. Caspase-2 mediated apoptotic and necrotic murine macrophage cell death induced by rough Brucella abortus. PLoS ONE. 2009;4:e6830 pubmed publisher
    ..The biological relevance of Brucella O antigen and caspase-2-mediated macrophage cell death in Brucella pathogenesis and protective Brucella immunity is discussed. ..
  28. Archambaud C, Salcedo S, Lelouard H, Devilard E, de Bovis B, Van Rooijen N, et al. Contrasting roles of macrophages and dendritic cells in controlling initial pulmonary Brucella infection. Eur J Immunol. 2010;40:3458-71 pubmed publisher
    ..cytometry, we analyzed in the mouse the initial innate immune response that follows intranasal inoculation of Brucella abortus. Bacteria were absent from parenchymal dendritic cells (DC) but present in alveolar macrophages in which they ..
  29. Ko J, Splitter G. Brucella abortus tandem repeated ATP-binding proteins, BapA and BapB, homologs of haemophilus influenzae LktB, are not necessary for intracellular survival. Microb Pathog. 2000;29:245-53 pubmed
    b>Brucella abortus actively secretes materials and uptakes nutrients to maintain the survival and multiplication of the bacteria in host cells...
  30. Cabrera A, Sáez D, Céspedes S, Andrews E, Oñate A. Vaccination with recombinant Semliki Forest virus particles expressing translation initiation factor 3 of Brucella abortus induces protective immunity in BALB/c mice. Immunobiology. 2009;214:467-74 pubmed publisher
    ..We constructed infectious but replication-deficient SFV particles carrying recombinant RNA encoding the Brucella abortus translation initiation factor 3 (IF3)...
  31. Ferreira A, Chambel L, Tenreiro T, Cardoso R, Flor L, Dias I, et al. MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates. PLoS ONE. 2012;7:e42514 pubmed publisher
    ..population, confirming epidemiological linkage in outbreak investigations. In particular, it suggests recent and ongoing colonisation of Portugal with one B. melitensis lineage usually associated with East Mediterranean countries...
  32. Li X, He Y. Caspase-2-dependent dendritic cell death, maturation, and priming of T cells in response to Brucella abortus infection. PLoS ONE. 2012;7:e43512 pubmed publisher
    Smooth virulent Brucella abortus strain 2308 (S2308) causes zoonotic brucellosis in cattle and humans. Rough B...
  33. Muma J, Godfroid J, Samui K, Skjerve E. The role of Brucella infection in abortions among traditional cattle reared in proximity to wildlife on the Kafue flats of Zambia. Rev Sci Tech. 2007;26:721-30 pubmed
    ..These results establish that Brucella infections contribute significantly to cattle abortions in the traditional livestock sector of Zambia...
  34. Pei J, Turse J, Ficht T. Evidence of Brucella abortus OPS dictating uptake and restricting NF-kappaB activation in murine macrophages. Microbes Infect. 2008;10:582-90 pubmed publisher
    Smooth Brucella abortus S2308 is virulent while rough derivatives are attenuated. Intracellular killing is often blamed for these differences...
  35. Carrica M, Fernandez I, Sieira R, Paris G, Goldbaum F. The two-component systems PrrBA and NtrYX co-ordinately regulate the adaptation of Brucella abortus to an oxygen-limited environment. Mol Microbiol. 2013;88:222-33 pubmed publisher
    ..In this report we describe a regulatory network involved in this adaptation. We show that Brucella abortus?PrrBA is a functional two-component signal transduction system that responds to the redox status and acts as ..
  36. Skyberg J, Thornburg T, Rollins M, Huarte E, Jutila M, Pascual D. Murine and bovine ?? T cells enhance innate immunity against Brucella abortus infections. PLoS ONE. 2011;6:e21978 pubmed publisher
    ..abortus via IFN-?. Collectively, these results demonstrate ?? T cells are important for early protection to B. abortus infections...
  37. Her M, Kang S, Kim J, Kim J, Hwang I, Jung S, et al. A genetic comparison of Brucella abortus isolates from animals and humans by using an MLVA assay. J Microbiol Biotechnol. 2010;20:1750-5 pubmed
    ..This study represents the first data of genetic correlation of B. abortus isolates from humans and animals in South Korea...
  38. Paschos A, den Hartigh A, Smith M, Atluri V, Sivanesan D, Tsolis R, et al. An in vivo high-throughput screening approach targeting the type IV secretion system component VirB8 identified inhibitors of Brucella abortus 2308 proliferation. Infect Immun. 2011;79:1033-43 pubmed publisher
    ..reduced the levels of VirB8 and of other VirB proteins, and many of them inhibited virB gene transcription in Brucella abortus 2308, suggesting that targeting of the secretion system has complex regulatory effects in vivo...
  39. Barrionuevo P, Delpino M, Velásquez L, García Samartino C, Coria L, Ibañez A, et al. Brucella abortus inhibits IFN-?-induced Fc?RI expression and Fc?RI-restricted phagocytosis via toll-like receptor 2 on human monocytes/macrophages. Microbes Infect. 2011;13:239-50 pubmed publisher
    The strategies that allow Brucella abortus to persist for years inside macrophages subverting host immune responses are not completely understood...
  40. Sáez D, Fernandez P, Rivera A, Andrews E, Oñate A. Oral immunization of mice with recombinant Lactococcus lactis expressing Cu,Zn superoxide dismutase of Brucella abortus triggers protective immunity. Vaccine. 2012;30:1283-90 pubmed publisher
    ..Here, we evaluated the usefulness of recombinant Lactococcus lactis secreting Brucella abortus Cu-Zn superoxide dismutase (SOD) as oral antigen delivery system, when administered alone or in combination ..
  41. Perez Gil J, Calisto B, Behrendt C, Kurz T, Fita I, Rodríguez Concepción M. Crystal structure of Brucella abortus deoxyxylulose-5-phosphate reductoisomerase-like (DRL) enzyme involved in isoprenoid biosynthesis. J Biol Chem. 2012;287:15803-9 pubmed publisher
    ..Here, we report the x-ray crystal structures of the Brucella abortus DRL enzyme in its apo form and in complex with the broad-spectrum antibiotic fosmidomycin solved to 1.5 and 1...
  42. Ficht T, Kahl McDonagh M, Arenas Gamboa A, Rice Ficht A. Brucellosis: the case for live, attenuated vaccines. Vaccine. 2009;27 Suppl 4:D40-3 pubmed publisher
    ..Based upon preliminary data, it is postulated that such vaccine delivery systems can be storage stable, administered orally or intranasally, and generally applicable to a number of agents...
  43. Maichak E, Scurlock B, Rogerson J, Meadows L, Barbknecht A, Edwards W, et al. Effects of management, behavior, and scavenging on risk of brucellosis transmission in elk of western Wyoming. J Wildl Dis. 2009;45:398-410 pubmed
  44. Fu S, Xu J, Li X, Xie Y, Qiu Y, Du X, et al. Immunization of mice with recombinant protein CobB or AsnC confers protection against Brucella abortus infection. PLoS ONE. 2012;7:e29552 pubmed publisher
    ..Altogether, the present study showed that CobB or AsnC protein could be useful antigen candidates for the development of subunit vaccines against brucellosis with adequate immunogenicity and protection efficacy...
  45. Jain S, Afley P, Kumar S. Immunological responses to recombinant cysteine synthase A of Brucella abortus in BALB/c mice. World J Microbiol Biotechnol. 2013;29:907-13 pubmed publisher
    ..In the present study, we generated recombinant cysteine synthase A (rCysK) of Brucella abortus in Escherichia coli and purified it up to homogeneity by metal affinity chromatography...
  46. Zhang C, Bai N, Zhang Z, Liang N, Dong L, Xiang R, et al. TLR2 signaling subpathways regulate TLR9 signaling for the effective induction of IL-12 upon stimulation by heat-killed Brucella abortus. Cell Mol Immunol. 2012;9:324-33 pubmed publisher
    b>Brucella abortus is a Gram-negative intracellular bacterium that induces MyD88-dependent IL-12 production in dentritic cells (DCs) and a subsequent protective Th1 immune response...
  47. Menscher E, Caswell C, Anderson E, Roop R. Mur regulates the gene encoding the manganese transporter MntH in Brucella abortus 2308. J Bacteriol. 2012;194:561-6 pubmed publisher
    ..A previous study showed that MntH is required for the wild-type virulence of Brucella abortus 2308 in mice (Anderson ES, et al., Infect. Immun...
  48. Jain N, Rodriguez A, Kimsawatde G, Seleem M, Boyle S, Sriranganathan N. Effect of entF deletion on iron acquisition and erythritol metabolism by Brucella abortus 2308. FEMS Microbiol Lett. 2011;316:1-6 pubmed publisher
    b>Brucella abortus has been shown to produce two siderophores: 2,3-dihydroxybenzoic acid (2,3-DHBA) and brucebactin...
  49. Lamontagne J, Butler H, Chaves Olarte E, Hunter J, Schirm M, Paquet C, et al. Extensive cell envelope modulation is associated with virulence in Brucella abortus. J Proteome Res. 2007;6:1519-29 pubmed
    ..spectrometry-based proteomic analysis of spontaneously released outer membrane fragments from four strains of Brucella abortus (wild type virulent, avirulent bvrR- and bvrS- mutants as well as reconstituted virulent bvrR+ (bvrR-/pbvrR+))..
  50. Oliveira S, de Almeida L, Carvalho N, Oliveira F, Lacerda T. Update on the role of innate immune receptors during Brucella abortus infection. Vet Immunol Immunopathol. 2012;148:129-35 pubmed publisher
    ..It was demonstrated that TLR2 clearly does not play any role in controlling Brucella abortus infection in vivo, whereas TLR9 has been shown to be required for clearance of this bacterium in infected ..
  51. Delpino M, Comerci D, Wagner M, Eschenbrenner M, Mujer C, Ugalde R, et al. Differential composition of culture supernatants from wild-type Brucella abortus and its isogenic virB mutants. Arch Microbiol. 2009;191:571-81 pubmed publisher
    ..These results suggest that the expression of virB genes affects the extracellular release of DnaK, PPIase and CGH, and possibly other proteins from B. abortus...
  52. Delpino M, Barrionuevo P, Macedo G, Oliveira S, Genaro S, Scian R, et al. Macrophage-elicited osteoclastogenesis in response to Brucella abortus infection requires TLR2/MyD88-dependent TNF-? production. J Leukoc Biol. 2012;91:285-98 pubmed publisher
    ..In this manuscript, we described an immune mechanism for inflammatory bone loss in response to infection by Brucella abortus. We established a requirement for MyD88 and TLR2 in TNF-?-elicited osteoclastogenesis in response to B...
  53. Elhassanny A, Anderson E, Menscher E, Roop R. The ferrous iron transporter FtrABCD is required for the virulence of Brucella abortus 2308 in mice. Mol Microbiol. 2013;88:1070-82 pubmed publisher
    ..The genes designated BAB2_0837-0840 in the Brucella abortus 2308 genome sequence are predicted to encode a CupII-type ferrous iron transporter homologous to the FtrABCD ..
  54. Schumaker B. Risks of Brucella abortus spillover in the Greater Yellowstone area. Rev Sci Tech. 2013;32:71-7 pubmed
    Recurrent spillover of Brucella abortus from wildlife reservoirs to domestic cattle in the Greater Yellowstone Area (GYA) has prevented the United States from completely eradicating bovine brucellosis...
  55. Capparelli R, Alfano F, Amoroso M, Borriello G, Fenizia D, Bianco A, et al. Protective effect of the Nramp1 BB genotype against Brucella abortus in the water buffalo (Bubalus bubalis). Infect Immun. 2007;75:988-96 pubmed
    We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary ..
  56. Paixao T, Poester F, Carvalho Neta A, Borges A, Lage A, Santos R. NRAMP1 3' untranslated region polymorphisms are not associated with natural resistance to Brucella abortus in cattle. Infect Immun. 2007;75:2493-9 pubmed
    ..Taken together, these results indicate that these polymorphisms at the NRAMP1 3'UTR do not affect resistance against B. abortus in cattle and that they are therefore not suitable markers of natural resistance against bovine brucellosis...
  57. Scian R, Barrionuevo P, Giambartolomei G, Fossati C, Baldi P, Delpino M. Granulocyte-macrophage colony-stimulating factor- and tumor necrosis factor alpha-mediated matrix metalloproteinase production by human osteoblasts and monocytes after infection with Brucella abortus. Infect Immun. 2011;79:192-202 pubmed publisher
    ..infectious diseases, we investigated the production of MMPs by human osteoblasts and monocytes, either upon Brucella abortus infection or upon reciprocal stimulation with factors produced by each infected cell type. B...
  58. Rossetti C, Galindo C, Everts R, Lewin H, Garner H, Adams L. Comparative analysis of the early transcriptome of Brucella abortus--infected monocyte-derived macrophages from cattle naturally resistant or susceptible to brucellosis. Res Vet Sci. 2011;91:40-51 pubmed publisher
    ..abortus infection in R MDMs while the cell cycle was largely arrested in infected S MDMs at 12 h p.i. These results contribute to an improved understanding of how host responses may be manipulated to prevent infection by brucellae...
  59. Carvalho Neta A, Stynen A, Paixao T, Miranda K, Silva F, Roux C, et al. Modulation of the bovine trophoblastic innate immune response by Brucella abortus. Infect Immun. 2008;76:1897-907 pubmed publisher
    Brucellosis is still a widespread zoonotic disease. Very little is known about the interaction between Brucella abortus and trophoblastic cells, which is essential for better understanding the pathogenesis of the Brucella-induced ..
  60. Lamontagne J, Béland M, Forest A, Côté Martin A, Nassif N, Tomaki F, et al. Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome. BMC Genomics. 2010;11:300 pubmed publisher
    ..While conducting proteomics studies to better understand Brucella abortus virulence, we consolidated the proteomic data collected and compared it to publically available genomic data.
  61. Amirmozafari N, Ghazi F, Mostafazadeh A, Mostafaie A, Rajabnia R. Comparison of heat shock response in Brucella abortus and Brucella melitensis. Pak J Biol Sci. 2008;11:188-94 pubmed
    ..Therefore, hsp-60 can be a good antigenic candidate for engineering subunit vaccine against Brucella, as well as for ELISA test development...
  62. Yang X, Thornburg T, Walters N, Pascual D. DeltaznuADeltapurE Brucella abortus 2308 mutant as a live vaccine candidate. Vaccine. 2010;28:1069-74 pubmed publisher
    To create a new, safe brucellosis live vaccine, a double mutant strain was constructed from Brucella abortus 2308. Using the DeltaznuA B. abortus 2308 mutant, a second mutation was introduced by deleting purE gene...
  63. Giambartolomei G, Scian R, Acosta Rodríguez E, Fossati C, Delpino M. Brucella abortus-infected macrophages modulate T lymphocytes to promote osteoclastogenesis via IL-17. Am J Pathol. 2012;181:887-96 pubmed publisher
    ..We present in vitro and in vivo evidence showing that Brucella abortus-induced inflammatory response leads to the activation of LTs, which further promote osteoclastogenesis...
  64. Miyoshi A, Rosinha G, Camargo I, Trant C, Cardoso F, Azevedo V, et al. The role of the vacB gene in the pathogenesis of Brucella abortus. Microbes Infect. 2007;9:375-81 pubmed
    ..In this study, we have identified Brucella abortus vacB gene that presents 2343 nucleotides and 781 amino acids and it shows 39% identity with Shigella flexneri ..
  65. Barrionuevo P, Cassataro J, Delpino M, Zwerdling A, Pasquevich K, García Samartino C, et al. Brucella abortus inhibits major histocompatibility complex class II expression and antigen processing through interleukin-6 secretion via Toll-like receptor 2. Infect Immun. 2008;76:250-62 pubmed
    The strategies that allow Brucella abortus to survive inside macrophages for prolonged periods and to avoid the immunological surveillance of major histocompatibility complex class II (MHC-II)-restricted gamma interferon (IFN-gamma)-..
  66. Crasta O, Folkerts O, Fei Z, Mane S, Evans C, Martino Catt S, et al. Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes. PLoS ONE. 2008;3:e2193 pubmed publisher
    The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as a vaccine strain in vaccination of cattle against brucellosis for six decades...
  67. Anderson E, Paulley J, Gaines J, Valderas M, Martin D, Menscher E, et al. The manganese transporter MntH is a critical virulence determinant for Brucella abortus 2308 in experimentally infected mice. Infect Immun. 2009;77:3466-74 pubmed publisher
    The gene designated BAB1_1460 in the Brucella abortus 2308 genome sequence is predicted to encode the manganese transporter MntH...
  68. de Barsy M, Jamet A, Filopon D, Nicolas C, Laloux G, Rual J, et al. Identification of a Brucella spp. secreted effector specifically interacting with human small GTPase Rab2. Cell Microbiol. 2011;13:1044-58 pubmed publisher
    ..A TEM-?-lactamase-RicA fusion was translocated from Brucella abortus to RAW264.7 macrophages during infection...
  69. Caswell C, Gaines J, Roop R. The RNA chaperone Hfq independently coordinates expression of the VirB type IV secretion system and the LuxR-type regulator BabR in Brucella abortus 2308. J Bacteriol. 2012;194:3-14 pubmed publisher
    ..and the present study links the RNA chaperone Hfq to wild-type expression of virB in Brucella abortus 2308...
  70. Bonomi H, Marchesini M, Klinke S, Ugalde J, Zylberman V, Ugalde R, et al. An atypical riboflavin pathway is essential for Brucella abortus virulence. PLoS ONE. 2010;5:e9435 pubmed publisher
    ..In summary, we show that riboflavin biosynthesis is essential for Brucella survival inside cells or in mice. These results highlight the potential use of flavin biosynthetic pathway enzymes as targets for the chemotherapy of brucellosis...
  71. Lapaque N, Muller A, Alexopoulou L, Howard J, Gorvel J. Brucella abortus induces Irgm3 and Irga6 expression via type-I IFN by a MyD88-dependent pathway, without the requirement of TLR2, TLR4, TLR5 and TLR9. Microb Pathog. 2009;47:299-304 pubmed publisher
    ..Here, we show in murine macrophages that Brucella abortus triggers expression of the interferon-inducible resistance proteins (IRGs, p47 GTPases) via type-I IFN ..
  72. Lacerda T, Cardoso P, Augusto de Almeida L, Camargo I, Afonso D, Trant C, et al. Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice. Vaccine. 2010;28:5627-34 pubmed publisher
    Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B...
  73. Barquero Calvo E, Chaves Olarte E, Weiss D, Guzmán Verri C, Chacón Díaz C, Rucavado A, et al. Brucella abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection. PLoS ONE. 2007;2:e631 pubmed
    To unravel the strategy by which Brucella abortus establishes chronic infections, we explored its early interaction with innate immunity.
  74. Dotreppe D, Mullier C, Letesson J, De Bolle X. The alkylation response protein AidB is localized at the new poles and constriction sites in Brucella abortus. BMC Microbiol. 2011;11:257 pubmed publisher
    b>Brucella abortus is the etiological agent of a worldwide zoonosis called brucellosis. This alpha-proteobacterium is dividing asymmetrically, and PdhS, an essential histidine kinase, was reported to be an old pole marker.
  75. den Hartigh A, Rolán H, de Jong M, Tsolis R. VirB3 to VirB6 and VirB8 to VirB11, but not VirB7, are essential for mediating persistence of Brucella in the reticuloendothelial system. J Bacteriol. 2008;190:4427-36 pubmed publisher
    The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B...
  76. Pollak C, Delpino M, Fossati C, Baldi P. Outer membrane vesicles from Brucella abortus promote bacterial internalization by human monocytes and modulate their innate immune response. PLoS ONE. 2012;7:e50214 pubmed publisher
    ..The aim of the present study was to assess the interaction of OMVs from Brucella abortus with human epithelial cells (HeLa) and monocytes (THP-1), and the potential immunomodulatory effects they may ..
  77. Gomes M, Campos P, de Almeida L, Oliveira F, Costa M, Marim F, et al. The role of innate immune signals in immunity to Brucella abortus. Front Cell Infect Microbiol. 2012;2:130 pubmed publisher
    ..Our research group has been using the Gram-negative bacteria Brucella abortus as a model of pathogen. We have demonstrated that B...
  78. Sun Y, de Jong M, den Hartigh A, Roux C, Rolán H, Tsolis R. The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus. Front Cell Infect Microbiol. 2012;2:47 pubmed publisher
    ..number of hypothetical genes potentially encoding small proteins of unknown function are annotated in the Brucella abortus genome...
  79. Marchesini M, Herrmann C, Salcedo S, Gorvel J, Comerci D. In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system. Cell Microbiol. 2011;13:1261-74 pubmed publisher
    ..We confirmed VirB-mediated translocation of one of the substrates by immunofluorescence confocal microscopy, and we found that the N-terminal 25 amino acids are required for its delivery into cells...
  80. Pakzad I, Rezaee A, Rasaee M, Tabbaraee B, Delpisheh A. Immunogencity of HSA-L7/L12 (Brucella abortus ribosomal protein) in an animal model. Iran J Immunol. 2009;6:12-21 pubmed publisher
    The immunogenic Brucella abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis.
  81. Olsen S, Tatum F. Bovine brucellosis. Vet Clin North Am Food Anim Pract. 2010;26:15-27, table of contents pubmed publisher
    Infection of cattle caused by Brucella abortus (ie, bovine brucellosis) has been of political importance in the United States for many decades...
  82. Fugier E, Salcedo S, de Chastellier C, Pophillat M, Muller A, Arce Gorvel V, et al. The glyceraldehyde-3-phosphate dehydrogenase and the small GTPase Rab 2 are crucial for Brucella replication. PLoS Pathog. 2009;5:e1000487 pubmed publisher
    The intracellular pathogen Brucella abortus survives and replicates inside host cells within an endoplasmic reticulum (ER)-derived replicative organelle named the "Brucella-containing vacuole" (BCV)...
  83. Rhyan J, Aune K, Roffe T, Ewalt D, Hennager S, Gidlewski T, et al. Pathogenesis and epidemiology of brucellosis in yellowstone bison: serologic and culture results from adult females and their progeny. J Wildl Dis. 2009;45:729-39 pubmed
    Our objective in this prospective study was to determine the natural course of Brucella abortus infection in cohorts of seropositive and seronegative, female bison (Bison bison) and their offspring in Yellowstone National Park (YNP) for 5 ..
  84. Ramos T, Pinheiro Júnior J, Moura Sobrinho P, Santana V, Guerra N, de Melo L, et al. Epidemiological aspects of an infection by Brucella abortus in risk occupational groups in the microregion of Araguaína, Tocantins. Braz J Infect Dis. 2008;12:133-8 pubmed
    The aim of this paper was to study some epidemiological aspects of the infection by Brucella abortus in risk occupational groups in the microregion of Araguaína, Tocantins...
  85. Wallach J, Ferrero M, Victoria Delpino M, Fossati C, Baldi P. Occupational infection due to Brucella abortus S19 among workers involved in vaccine production in Argentina. Clin Microbiol Infect. 2008;14:805-7 pubmed publisher
    The pathological consequences of exposure to the vaccine strain Brucella abortus S19 were evaluated in 30 employees from vaccine-manufacturing plants...
  86. Billard E, Dornand J, Gross A. Interaction of Brucella suis and Brucella abortus rough strains with human dendritic cells. Infect Immun. 2007;75:5916-23 pubmed
    ..we report that, in contrast to smooth bacteria, infection of DCs with rough mutants of Brucella suis or Brucella abortus leads to both phenotypic and functional maturation of infected cells...
  87. Kim H, Caswell C, Foreman R, Roop R, Crosson S. The Brucella abortus general stress response system regulates chronic mammalian infection and is controlled by phosphorylation and proteolysis. J Biol Chem. 2013;288:13906-16 pubmed publisher
    ..Virulence of pathogenic bacteria is often determined by their ability to adapt to stress...
  88. Xavier M, Winter M, Spees A, Nguyen K, Atluri V, Silva T, et al. CD4+ T cell-derived IL-10 promotes Brucella abortus persistence via modulation of macrophage function. PLoS Pathog. 2013;9:e1003454 pubmed publisher
    ..Here, we show that the persistent intracellular pathogen Brucella abortus prevents immune activation of macrophages by inducing CD4(+)CD25(+) T cells to produce the anti-inflammatory ..
  89. Ramamoorthy S, Sanakkayala N, Vemulapalli R, Jain N, Lindsay D, Schurig G, et al. Prevention of vertical transmission of Neospora caninum in C57BL/6 mice vaccinated with Brucella abortus strain RB51 expressing N. caninum protective antigens. Int J Parasitol. 2007;37:1531-8 pubmed
    ..b>Brucella abortus strain RB51, a commercially available vaccine for bovine brucellosis, can also be used as a vector to express ..
  90. Roux C, Rolán H, Santos R, Beremand P, Thomas T, Adams L, et al. Brucella requires a functional Type IV secretion system to elicit innate immune responses in mice. Cell Microbiol. 2007;9:1851-69 pubmed
    ..Thus, induction of type I interferons does not appear to be an essential mechanism by which the T4SS promotes persistent infection by Brucella...
  91. Oliveira F, Carvalho N, Brandão A, Gomes M, de Almeida L, Oliveira S. Interleukin-1 receptor-associated kinase 4 is essential for initial host control of Brucella abortus infection. Infect Immun. 2011;79:4688-95 pubmed publisher
    b>Brucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. Recent studies have revealed that Toll-like receptor (TLR)-initiated immune response to Brucella spp...