brucella

Summary

Summary: A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.

Top Publications

  1. Ocampo Sosa A, García Lobo J. Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis. BMC Microbiol. 2008;8:17 pubmed publisher
    The Brucella genome contains an insertion sequence (IS) element called IS711 or IS6501, which is specific to the genus. The copy number of IS711 varies in the genome of the different Brucella species, ranging from 7 in B. abortus, B...
  2. Jauniaux T, Brenez C, Fretin D, Godfroid J, Haelters J, Jacques T, et al. Brucella ceti infection in harbor porpoise (Phocoena phocoena). Emerg Infect Dis. 2010;16:1966-8 pubmed publisher
    We describe Brucella sp. infection and associated lesions in a harbor porpoise (Phocoena phocoena) found on the coast of Belgium...
  3. Scholz H, Hofer E, Vergnaud G, Le Fleche P, Whatmore A, Al Dahouk S, et al. Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. Vector Borne Zoonotic Dis. 2009;9:153-6 pubmed publisher
    ..Cells were fast growing, nonmotile, and agglutinated with monospecific anti-Brucella (M) serum. Both strains were biochemically identified as Ochrobactrum anthropi by using the API 20NE test...
  4. Araj G. Update on laboratory diagnosis of human brucellosis. Int J Antimicrob Agents. 2010;36 Suppl 1:S12-7 pubmed publisher
    ..clinicians must use a wide range of non-specific routine haematological and biochemical tests in addition to Brucella-specific assays. The latter are microbiological (culture), serological (e.g...
  5. Fischer D, Lorenz N, Heuser W, Kampfer P, Scholz H, Lierz M. Abscesses associated with a Brucella inopinata-like bacterium in a big-eyed tree frog (Leptopelis vermiculatus). J Zoo Wildl Med. 2012;43:625-8 pubmed
    ..The two abscess-like masses were surgically opened, and a whitish-yellow pasty content was removed. A Brucella inopinata-like bacterium was obtained in pure culture and was resistant against ampicillin and tylosin but ..
  6. Todd T, Tibi O, Lin Y, Sayers S, Bronner D, Xiang Z, et al. Meta-analysis of variables affecting mouse protection efficacy of whole organism Brucella vaccines and vaccine candidates. BMC Bioinformatics. 2013;14 Suppl 6:S3 pubmed publisher
    ..Many variables can affect the results of vaccine protection. Brucella, a genus of facultative intracellular bacteria, is the etiologic agent of brucellosis in humans and multiple ..
  7. De B, Stauffer L, Koylass M, Sharp S, Gee J, Helsel L, et al. Novel Brucella strain (BO1) associated with a prosthetic breast implant infection. J Clin Microbiol. 2008;46:43-9 pubmed
    We report the microbiological, biochemical, and molecular characterization of an unusual Brucella strain (BO1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis...
  8. Prenger Berninghoff E, Siebert U, Stede M, Konig A, Weiss R, Baljer G. Incidence of Brucella species in marine mammals of the German North Sea. Dis Aquat Organ. 2008;81:65-71 pubmed publisher
    ..Phocoena phocoena, 34 grey seals Halichoerus grypus and 10 other marine mammals were assessed for the presence of Brucella species...
  9. Mancilla M, López Goñi I, Moriyon I, Zárraga A. Genomic island 2 is an unstable genetic element contributing to Brucella lipopolysaccharide spontaneous smooth-to-rough dissociation. J Bacteriol. 2010;192:6346-51 pubmed publisher
    b>Brucella is a Gram-negative bacterium that causes a worldwide-distributed zoonosis. The genus includes smooth (S) and rough (R) species that differ in the presence or absence, respectively, of the O-polysaccharide of lipopolysaccharide...

More Information

Publications102 found, 100 shown here

  1. Bounaadja L, Albert D, Chénais B, Hénault S, Zygmunt M, Poliak S, et al. Real-time PCR for identification of Brucella spp.: a comparative study of IS711, bcsp31 and per target genes. Vet Microbiol. 2009;137:156-64 pubmed publisher
    Culture is considered as the reference standard assay for diagnosis of Brucella spp. in humans and animals but it is time-consuming and hazardous...
  2. Dawson C, Stubberfield E, Perrett L, King A, Whatmore A, Bashiruddin J, et al. Phenotypic and molecular characterisation of Brucella isolates from marine mammals. BMC Microbiol. 2008;8:224 pubmed publisher
    Bacteria of the genus Brucella are the causative organisms of brucellosis in animals and man...
  3. Earhart K, Vafakolov S, Yarmohamedova N, Michael A, Tjaden J, Soliman A. Risk factors for brucellosis in Samarqand Oblast, Uzbekistan. Int J Infect Dis. 2009;13:749-53 pubmed publisher
    ..Structured questionnaires were completed and consent forms signed. Patients and controls were tested on site for Brucella infection by standard tube agglutination test and culture...
  4. Ding J, Pan Y, Jiang H, Cheng J, Liu T, Qin N, et al. Whole genome sequences of four Brucella strains. J Bacteriol. 2011;193:3674-5 pubmed publisher
    b>Brucella melitensis and Brucella suis are intracellular pathogens of livestock and humans. Here we report four genome sequences, those of the virulent strain B. melitensis M28-12 and vaccine strains B. melitensis M5 and M111 and B...
  5. De Bolle X, Letesson J, Gorvel J. Small GTPases and Brucella entry into the endoplasmic reticulum. Biochem Soc Trans. 2012;40:1348-52 pubmed publisher
    ..In the case of Brucella, the bacterium targets the ER (endoplasmic reticulum) to create a replicating niche called the BCV (Brucella-..
  6. Neimanis A, Koopman H, Westgate A, Nielsen K, Leighton F. Evidence of exposure to Brucella sp. in harbor porpoises (Phocoena phocoena) from the Bay of Fundy, Canada. J Wildl Dis. 2008;44:480-5 pubmed
    Novel strains of Brucella recently have been discovered in marine mammals...
  7. Delpino M, Fossati C, Baldi P. Proinflammatory response of human osteoblastic cell lines and osteoblast-monocyte interaction upon infection with Brucella spp. Infect Immun. 2009;77:984-95 pubmed publisher
    The ability of Brucella spp. to infect human osteoblasts and the cytokine response of these cells to infection were investigated in vitro. Brucella abortus, B. suis, B. melitensis, and B...
  8. Radhakrishnan G, Yu Q, Harms J, Splitter G. Brucella TIR Domain-containing Protein Mimics Properties of the Toll-like Receptor Adaptor Protein TIRAP. J Biol Chem. 2009;284:9892-8 pubmed publisher
    ..b>Brucella encodes a TIR domain-containing protein (TcpB) that inhibits TLR2- and TLR4-mediated NF-kappaB activation...
  9. Queipo Ortuño M, Colmenero J, Bermudez P, Bravo M, Morata P. Rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis using a multiplex real-time PCR assay. PLoS ONE. 2009;4:e4526 pubmed publisher
    ..multiplex real-time PCR assay (M RT-PCR) for the simultaneous detection of Mycobacterium tuberculosis complex and Brucella spp. Conventional microbiological techniques and M RT-PCR for M...
  10. Mayer Scholl A, Draeger A, Göllner C, Scholz H, Nockler K. Advancement of a multiplex PCR for the differentiation of all currently described Brucella species. J Microbiol Methods. 2010;80:112-4 pubmed publisher
    ..report a robust and rapid multiplex PCR assay, which allows for the differentiation of all nine currently recognised Brucella species. This includes the recently described species B. microti, B. inopinata, B. ceti and B. pinnipedialis.
  11. den Hartigh A, Rolán H, de Jong M, Tsolis R. VirB3 to VirB6 and VirB8 to VirB11, but not VirB7, are essential for mediating persistence of Brucella in the reticuloendothelial system. J Bacteriol. 2008;190:4427-36 pubmed publisher
    The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B...
  12. Scholz H, Hubalek Z, Nesvadbova J, Tomaso H, Vergnaud G, Le Fleche P, et al. Isolation of Brucella microti from soil. Emerg Infect Dis. 2008;14:1316-7 pubmed publisher
  13. Bayram Y, Korkoca H, Aypak C, Parlak M, Cikman A, Kilic S, et al. Antimicrobial susceptibilities of Brucella isolates from various clinical specimens. Int J Med Sci. 2011;8:198-202 pubmed
    ..In the study we claimed to identify Brucella species from clinical samples of patients with active brucellosis from Van region of Eastern Anatolia and to ..
  14. Baldi P, Giambartolomei G. Immunopathology of Brucella infection. Recent Pat Antiinfect Drug Discov. 2013;8:18-26 pubmed
    ..As Brucella lacks exotoxins, exoproteases or cytolysins, pathological findings in brucellosis probably arise from ..
  15. O Callaghan D, Whatmore A. Brucella genomics as we enter the multi-genome era. Brief Funct Genomics. 2011;10:334-41 pubmed publisher
    The genus Brucella includes species considered among the worlds most important zoonotic pathogens, with brucellosis remaining a significant problem in large parts of the world...
  16. Queipo Ortuño M, Colmenero J, Bravo M, García Ordoñez M, Morata P. Usefulness of a quantitative real-time PCR assay using serum samples to discriminate between inactive, serologically positive and active human brucellosis. Clin Microbiol Infect. 2008;14:1128-34 pubmed publisher
    ..b>Brucella spp...
  17. Jiménez de Bagüés M, Ouahrani Bettache S, Quintana J, Mitjana O, Hanna N, Bessoles S, et al. The new species Brucella microti replicates in macrophages and causes death in murine models of infection. J Infect Dis. 2010;202:3-10 pubmed publisher
    The recent isolation of Brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in Europe...
  18. Ficht T. Brucella taxonomy and evolution. Future Microbiol. 2010;5:859-66 pubmed publisher
    ..number of scientists attempting to reconcile new molecular data describing the phylogenetic relationship between Brucella organisms and a broader family of organisms with widely variant phenotypes that include human virulence and host ..
  19. Dawson C, Perrett L, Stubberfield E, Stack J, Farrelly S, Cooley W, et al. Isolation and characterization of Brucella from the lungworms of a harbor porpoise (Phocoena phocoena). J Wildl Dis. 2008;44:237-46 pubmed
    ..and molecular typing methods, immunologic and electron microscopy immunolabeling techniques, provided evidence of Brucella sp. infection within the uterine tissue of nematodes of this marine mammal...
  20. Matero P, Hemmilä H, Tomaso H, Piiparinen H, Rantakokko Jalava K, Nuotio L, et al. Rapid field detection assays for Bacillus anthracis, Brucella spp., Francisella tularensis and Yersinia pestis. Clin Microbiol Infect. 2011;17:34-43 pubmed publisher
    ..Assay sensitivities for B. anthracis, Brucella spp...
  21. Kilic S, Ivanov I, Durmaz R, Bayraktar M, Ayaslioglu E, Uyanik M, et al. Multiple-locus variable-number tandem-repeat analysis genotyping of human Brucella isolates from Turkey. J Clin Microbiol. 2011;49:3276-83 pubmed publisher
    ..analysis (MLVA) was applied to investigate the epidemiological relationship and genetic diversity among 162 human Brucella isolates collected from all geographic regions of Turkey in an 8-year period (2001 to 2008)...
  22. Sengupta D, Koblansky A, Gaines J, Brown T, West A, Zhang D, et al. Subversion of innate immune responses by Brucella through the targeted degradation of the TLR signaling adapter, MAL. J Immunol. 2010;184:956-64 pubmed publisher
    Gram-negative bacteria belonging to the Brucella species cause chronic infections that can result in undulant fever, arthritis, and osteomyelitis in humans. Remarkably, Brucella sp...
  23. Glynn M, Lynn T. Brucellosis. J Am Vet Med Assoc. 2008;233:900-8 pubmed publisher
  24. Hinić V, Brodard I, Thomann A, Holub M, Miserez R, Abril C. IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology. BMC Vet Res. 2009;5:22 pubmed publisher
    ..In the present study, we describe the evaluation of the recently established real-time PCR assay based on the Brucella-specific insertion sequence IS711 with blood samples from 199 wild boars (first group of animals) and tissue ..
  25. Obiako O, Ogoina D, Danbauchi S, Kwaifa S, Chom N, Nwokorie E. Neurobrucellosis--a case report and review of literature. Niger J Clin Pract. 2010;13:347-50 pubmed
    ..milk and brucellosis of the brain diagnosed in our centre through brain magnetic resonance imagining (MRI) and brucella antigen agglutination test...
  26. Barbier T, Nicolas C, Letesson J. Brucella adaptation and survival at the crossroad of metabolism and virulence. FEBS Lett. 2011;585:2929-34 pubmed publisher
    ..A typical example are the Brucella spp., facultative intracellular pathogens responsible for chronic infections of animals and humans...
  27. Bingham J, Taylor T, Swingler J, Meehan G, Middleton D, Mackereth G, et al. Infection trials in pigs with a human isolate of Brucella ( isolate 02/611 'marine mammal type' ). N Z Vet J. 2008;56:10-4 pubmed publisher
    To determine if pigs could support infection of a human Brucella isolate (Brucella 02/611) from New Zealand, and to study seroconversion to this isolate using a competitive ELISA. Ten weaner piglets were challenged with 4...
  28. Galińska E, Zagórski J. Brucellosis in humans--etiology, diagnostics, clinical forms. Ann Agric Environ Med. 2013;20:233-8 pubmed
    ..The etiologic factors of brucellosis are small, aerobic Gram-negative rods of the genus Brucella, which currently contains ten species: B. abortus, B. suis, B. ovis, B. melitensis, B. canis, B. neotomae, B...
  29. Hernández Mora G, González Barrientos R, Morales J, Chaves Olarte E, Guzmán Verri C, Barquero Calvo E, et al. Neurobrucellosis in stranded dolphins, Costa Rica. Emerg Infect Dis. 2008;14:1430-3 pubmed publisher
    ..coeruleoalba, stranded along the Costa Rican Pacific coast, had meningoencephalitis and antibodies against Brucella spp. Brucella ceti was isolated from cerebrospinal fluid of 6 dolphins and 1 fetus. S...
  30. Skendros P, Pappas G, Boura P. Cell-mediated immunity in human brucellosis. Microbes Infect. 2011;13:134-42 pubmed publisher
    b>Brucella can parasitize within human antigen-presenting cells modifying phagocytosis, phagolysosome fusion, antigen presentation, cytokine secretion, and apoptosis...
  31. Nymo I, Tryland M, Godfroid J. A review of Brucella infection in marine mammals, with special emphasis on Brucella pinnipedialis in the hooded seal (Cystophora cristata). Vet Res. 2011;42:93 pubmed publisher
    b>Brucella spp. were isolated from marine mammals for the first time in 1994. Two novel species were later included in the genus; Brucella ceti and Brucella pinnipedialis, with cetaceans and seals as their preferred hosts, respectively...
  32. Hubalek Z, Scholz H, Sedláček I, Melzer F, Sanogo Y, Nesvadbova J. Brucellosis of the common vole (Microtus arvalis). Vector Borne Zoonotic Dis. 2007;7:679-87 pubmed
    ..A) gene sequencing analysis of two isolates (CCM 4915=CAPM 6434; CCM 4916=CAPM 6435) identified them as Brucella sp. with sequence identities of 100% to other Brucella spp...
  33. Holt H, Eltholth M, Hegazy Y, El Tras W, Tayel A, Guitian J. Brucella spp. infection in large ruminants in an endemic area of Egypt: cross-sectional study investigating seroprevalence, risk factors and livestock owner's knowledge, attitudes and practices (KAPs). BMC Public Health. 2011;11:341 pubmed publisher
    ..It was first reported in Egypt in 1939 and is now endemic, the predominate species of Brucella in cattle and buffalo in Egypt is B. melitensis...
  34. Sieira R. Regulation of virulence in Brucella: an eclectic repertoire of transcription factors defines the complex architecture of the virB promoter. Future Microbiol. 2013;8:1193-208 pubmed publisher
    ..The genus Brucella comprises facultative intracellular bacteria that cause brucellosis, a disease affecting a wide range of mammals ..
  35. Martín Martín A, Sancho P, Tejedor C, Fernández Lago L, Vizcaino N. Differences in the outer membrane-related properties of the six classical Brucella species. Vet J. 2011;189:103-5 pubmed publisher
    ..and susceptibility to various compounds) of strains representative of the six classical species of the genus Brucella were assessed...
  36. De Miguel M, Marin C, Muñoz P, Dieste L, Grillo M, Blasco J. Development of a selective culture medium for primary isolation of the main Brucella species. J Clin Microbiol. 2011;49:1458-63 pubmed publisher
    ..However, despite inhibiting most contaminating microorganisms, FM also inhibits the growth of Brucella ovis and some B. melitensis and B. abortus strains...
  37. Hur J, Xiang Z, Feldman E, He Y. Ontology-based Brucella vaccine literature indexing and systematic analysis of gene-vaccine association network. BMC Immunol. 2011;12:49 pubmed publisher
    ..As a test case, we have examined vaccines for Brucella, the causative agent of brucellosis in humans and animals.
  38. Ari M, Guracha A, Fadeel M, Njuguna C, Njenga M, Kalani R, et al. Challenges of establishing the correct diagnosis of outbreaks of acute febrile illnesses in Africa: the case of a likely Brucella outbreak among nomadic pastoralists, northeast Kenya, March-July 2005. Am J Trop Med Hyg. 2011;85:909-12 pubmed publisher
    ..All were negative for viral and typhoid serology. Nine patients had Brucella antibodies present by at least one of the tests, four of whom had evidence suggestive of acute infection by the ..
  39. Mancilla M, Marin C, Blasco J, Zárraga A, López Goñi I, Moriyon I. Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies. J Bacteriol. 2012;194:1860-7 pubmed publisher
    The brucellae are Gram-negative pathogens that cause brucellosis, a zoonosis of worldwide importance. The genus Brucella includes smooth and rough species that differ in that they carry smooth and rough lipopolysaccharides, respectively...
  40. Asaad A, Alqahtani J. Serological and molecular diagnosis of human brucellosis in Najran, Southwestern Saudi Arabia. J Infect Public Health. 2012;5:189-94 pubmed publisher
    ..cases and controls were subjected to culture, standard tube agglutination test (SAT), ELISA for IgM and IgG, and brucella PCR. The diagnosis of brucellosis was confirmed in 54 (15...
  41. He Y. Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics. Front Cell Infect Microbiol. 2012;2:2 pubmed publisher
    b>Brucella is a Gram-negative, facultative intracellular bacterium that causes zoonotic brucellosis in humans and various animals. Out of 10 classified Brucella species, B. melitensis, B. abortus, B. suis, and B...
  42. Mazeri S, Scolamacchia F, Handel I, Morgan K, Tanya V, Bronsvoort B. Risk factor analysis for antibodies to Brucella, Leptospira and C. burnetii among cattle in the Adamawa Region of Cameroon: a cross-sectional study. Trop Anim Health Prod. 2013;45:617-23 pubmed publisher
    ..in cattle in the Adamawa Region of Cameroon, with wildlife, namely, buffaloes, playing a major role in both Brucella and Coxiella burnetii seropositivity...
  43. Garofolo G, Ancora M, Di Giannatale E. MLVA-16 loci panel on Brucella spp. using multiplex PCR and multicolor capillary electrophoresis. J Microbiol Methods. 2013;92:103-7 pubmed publisher
    ..panel (MLVA-16), involving singleplex PCRs and agarose gel electrophoresis, is the standard genotyping method for Brucella spp., used also for the Brucella international online database...
  44. Alvarez P, Zylberman V, Ghersi G, Boado L, Palacios C, Goldbaum F, et al. Tandem repeats of the extracellular domain of Matrix 2 influenza protein exposed in Brucella lumazine synthase decameric carrier molecule induce protection in mice. Vaccine. 2013;31:806-12 pubmed publisher
    ..In this work we evaluated the possible use of Brucella abortus lumazine synthase protein (BLS), a highly immunogenic decameric protein, as a carrier of the M2e peptide...
  45. Mai H, Irons P, Kabir J, Thompson P. Herd-level risk factors for Campylobacter fetus infection, Brucella seropositivity and within-herd seroprevalence of brucellosis in cattle in northern Nigeria. Prev Vet Med. 2013;111:256-67 pubmed publisher
    ..Serum from 4745 mature animals was tested for Brucella antibodies using the Rose-Bengal plate test and positives were confirmed in series-testing protocol using ..
  46. Whatmore A, Dawson C, Groussaud P, Koylass M, King A, Shankster S, et al. Marine mammal Brucella genotype associated with zoonotic infection. Emerg Infect Dis. 2008;14:517-8 pubmed publisher
  47. Bellido D, Craig P, Mozgovoj M, Gonzalez D, Wigdorovitz A, Goldbaum F, et al. Brucella spp. lumazine synthase as a bovine rotavirus antigen delivery system. Vaccine. 2009;27:136-45 pubmed publisher
    b>Brucella spp. lumazine synthase (BLS) is a highly immunogenic decameric protein. It has been previously evaluated as a carrier to increase the immunogenicity of peptides fused to its N-termini...
  48. Schlabritz Loutsevitch N, Whatmore A, Quance C, Koylass M, Cummins L, Dick E, et al. A novel Brucella isolate in association with two cases of stillbirth in non-human primates - first report. J Med Primatol. 2009;38:70-3 pubmed publisher
    ..Brucellosis is veterinary and human health problem...
  49. Mizanbayeva S, Smits H, Zhalilova K, Abdoel T, Kozakov S, Ospanov K, et al. The evaluation of a user-friendly lateral flow assay for the serodiagnosis of human brucellosis in Kazakhstan. Diagn Microbiol Infect Dis. 2009;65:14-20 pubmed publisher
    ..from Kazakhstan tested positive in the serum agglutination test for titers > or = 1:25 and reacted in the Brucella immunoglobulin M/immunoglobulin G lateral flow assay (LFA) confirming the high sensitivity of these assays...
  50. D Anastasio R, Staniscia T, Milia M, Manzoli L, Capasso L. Origin, evolution and paleoepidemiology of brucellosis. Epidemiol Infect. 2011;139:149-56 pubmed publisher
    ..Most paleopathological cases involve adult male skeletal individuals, and lumbar vertebrae and sacroiliac joints are most commonly involved...
  51. Bankole A, Saegerman C, Berkvens D, Fretin D, Geerts S, Ieven G, et al. Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia. Vet Rec. 2010;166:753-6 pubmed publisher
    ..of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species...
  52. Martirosyan A, Moreno E, Gorvel J. An evolutionary strategy for a stealthy intracellular Brucella pathogen. Immunol Rev. 2011;240:211-34 pubmed publisher
    b>Brucella is an intracellular bacterial pathogen that causes abortion and infertility in mammals and leads to a debilitating febrile illness that can progress into a long lasting disease with severe complications in humans...
  53. Buyukcangaz E, Sen A, Carli K, Kahya S. Comparison of direct culture versus PCR for the detection of Brucella in aborted fetuses of cattle and sheep in Turkey. Vet Rec. 2011;168:430 pubmed publisher
    The aim of this study was to detect Brucella in samples from aborted fetuses of sheep and cattle in Turkey using PCR and bacteriological analysis, and to determine the sensitivity and specificity of the PCR...
  54. Al Dahouk S, Nockler K. Implications of laboratory diagnosis on brucellosis therapy. Expert Rev Anti Infect Ther. 2011;9:833-45 pubmed publisher
    ..Whether the molecular detection of Brucella DNA in clinical samples should be followed by long-term antibiotic treatment or not is also a matter of debate...
  55. Song L, Li J, Hou S, Li X, Chen S. Establishment of loop-mediated isothermal amplification (LAMP) for rapid detection of Brucella spp. and application to milk and blood samples. J Microbiol Methods. 2012;90:292-7 pubmed publisher
    b>Brucella spp. are facultative intracellular bacteria that infect humans and animals. In this study, the loop-mediated isothermal amplification (LAMP) was used to detect the Brucella-specific gene omp25...
  56. Smith M, Coincon M, Paschos A, Jolicoeur B, Lavallée P, Sygusch J, et al. Identification of the binding site of Brucella VirB8 interaction inhibitors. Chem Biol. 2012;19:1041-8 pubmed publisher
    ..The type IV secretion system (T4SS) of the intracellular pathogen Brucella is a prerequisite for its virulence, and in this work we targeted the interactions of the essential assembly ..
  57. Queipo Ortuño M, Tena F, Colmenero J, Morata P. Comparison of seven commercial DNA extraction kits for the recovery of Brucella DNA from spiked human serum samples using real-time PCR. Eur J Clin Microbiol Infect Dis. 2008;27:109-14 pubmed
    ..Human serum samples were spiked with known concentrations of Brucella melitensis Rev 1; the DNA was extracted and tested in genus-specific LightCycler polymerase chain reaction (PCR)...
  58. Foster G, Osterman B, Godfroid J, Jacques I, Cloeckaert A. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol. 2007;57:2688-93 pubmed
    Small Gram-negative cocco-bacilli resembling Brucella strains have been reported from marine mammals since the mid-1990s...
  59. Cantu A, Ortega S J, Mosqueda J, Garcia Vazquez Z, Henke S, George J. Prevalence of infectious agents in free-ranging white-tailed deer in northeastern Mexico. J Wildl Dis. 2008;44:1002-7 pubmed
    ..6, 41.1, 63.5, and 0%, respectively, indicating that white-tailed deer and cattle may share disease agents when cohabiting in northeastern Mexico...
  60. Samaha H, Mohamed T, Khoudair R, Ashour H. Serodiagnosis of brucellosis in cattle and humans in Egypt. Immunobiology. 2009;214:223-6 pubmed publisher
    ..The high prevalence rates of human brucellosis call for more strict application of hygienic measures to prevent the spread of brucellosis from cattle and other livestock to humans...
  61. Wattam A, Williams K, Snyder E, Almeida N, Shukla M, Dickerman A, et al. Analysis of ten Brucella genomes reveals evidence for horizontal gene transfer despite a preferred intracellular lifestyle. J Bacteriol. 2009;191:3569-79 pubmed publisher
    The facultative intracellular bacterial pathogen Brucella infects a wide range of warm-blooded land and marine vertebrates and causes brucellosis...
  62. Ibrahim N, Belihu K, Lobago F, Bekana M. Sero-prevalence of bovine brucellosis and its risk factors in Jimma zone of Oromia Region, South-western Ethiopia. Trop Anim Health Prod. 2010;42:35-40 pubmed publisher
    ..Generally, the sero-prevalence of bovine brucellosis found in Jimma area was not high and the sero-prevalence was closely associated with some of the risk factors considered at individual animal and herd level...
  63. Whatmore A. Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. Infect Genet Evol. 2009;9:1168-84 pubmed publisher
    b>Brucella species are responsible for brucellosis, one of the world's most widespread zoonotic diseases causing abortion in domestic animals and a potentially debilitating infection of man...
  64. Liebes Y, Amir L, Marks R, Banai M. Immobilization strategies of Brucella particles on optical fibers for use in chemiluminescence immunosensors. Talanta. 2009;80:338-45 pubmed publisher
    ..This has led us to design a chemiluminescent immunosensor aimed at identifying anti-Brucella antibodies using optical fibers as the transducer...
  65. Hegazy Y, Moawad A, Osman S, Ridler A, Guitian J. Ruminant brucellosis in the Kafr El Sheikh Governorate of the Nile Delta, Egypt: prevalence of a neglected zoonosis. PLoS Negl Trop Dis. 2011;5:e944 pubmed publisher
    ..Our objectives are to estimate the frequency of ruminant brucellosis and to describe its spatial distribution in Kafr El Sheikh Governorate, Nile Delta, Egypt...
  66. Lynch M, Duignan P, Taylor T, Nielsen O, Kirkwood R, Gibbens J, et al. Epizootiology of Brucella infection in Australian fur seals. J Wildl Dis. 2011;47:352-63 pubmed
    Novel members of the bacterial genus Brucella have recently emerged as pathogens of various marine mammal species and as potential zoonotic agents...
  67. Hofer E, Revilla Fernández S, Al Dahouk S, Riehm J, Nockler K, Zygmunt M, et al. A potential novel Brucella species isolated from mandibular lymph nodes of red foxes in Austria. Vet Microbiol. 2012;155:93-9 pubmed publisher
    ..Here, we describe phenotypic and molecular characteristics of two atypical Brucella strains isolated from two foxes hunted 2008 in Eastern Austria...
  68. von Bargen K, Gorvel J, Salcedo S. Internal affairs: investigating the Brucella intracellular lifestyle. FEMS Microbiol Rev. 2012;36:533-62 pubmed publisher
    Bacteria of the genus Brucella are Gram-negative pathogens of several animal species that cause a zoonotic disease in humans known as brucellosis or Malta fever...
  69. Almendra C, Silva T, Beja Pereira A, Ferreira A, Ferrão Beck L, de Sá M, et al. "HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale. Infect Genet Evol. 2009;9:104-7 pubmed publisher
    We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal)...
  70. Foster J, Beckstrom Sternberg S, Pearson T, Beckstrom Sternberg J, Chain P, Roberto F, et al. Whole-genome-based phylogeny and divergence of the genus Brucella. J Bacteriol. 2009;191:2864-70 pubmed publisher
    ..We assessed the taxonomic and evolutionary relationships of five Brucella species-Brucella abortus, B. melitensis, B. suis, B. canis, and B. ovis-using whole-genome comparisons...
  71. Hernández Mora G, Manire C, González Barrientos R, Barquero Calvo E, Guzmán Verri C, Staggs L, et al. Serological diagnosis of Brucella infections in odontocetes. Clin Vaccine Immunol. 2009;16:906-15 pubmed publisher
    b>Brucella ceti causes disease in Odontoceti. The absence of control serum collections and the diversity of cetaceans have hampered the standardization of serological tests for the diagnosis of cetacean brucellosis...
  72. González Barrientos R, Morales J, Hernández Mora G, Barquero Calvo E, Guzman Verri C, Chaves Olarte E, et al. Pathology of striped dolphins (Stenella coeruleoalba) infected with Brucella ceti. J Comp Pathol. 2010;142:347-52 pubmed publisher
    ..coeruleoalba) displaying swimming disorders compatible with neurological syndromes were investigated for Brucella infection. Sixteen dolphins had meningoencephalomyelitis...
  73. Matope G, Bhebhe E, Muma J, Lund A, Skjerve E. Herd-level factors for Brucella seropositivity in cattle reared in smallholder dairy farms of Zimbabwe. Prev Vet Med. 2010;94:213-21 pubmed publisher
    A cross-sectional study was conducted to investigate factors for Brucella seropositivity in smallholder dairy cattle herds from Gokwe, Marirangwe, Mushagashe, Nharira, Rusitu and Wedza areas located in different agro-ecological regions of ..
  74. El Kholy A, Gomaa H, El Anany M, Abd El Rasheed E. Diagnosis of human brucellosis in Egypt by polymerase chain reaction. East Mediterr Health J. 2009;15:1068-74 pubmed
    ..We failed to extract Brucella DNA with a commercial kit, but an extraction kit designed in-house using 2 sets of primers [B4/B5 (223 bp) and ..
  75. Moustafa D, Garg V, Jain N, Sriranganathan N, Vemulapalli R. Immunization of mice with gamma-irradiated Brucella neotomae and its recombinant strains induces protection against virulent B. abortus, B. melitensis, and B. suis challenge. Vaccine. 2011;29:784-94 pubmed publisher
    ..a zoonotic disease of major public health concern in several developing countries, is primarily caused by Brucella abortus, Brucella melitensis, and Brucella suis. No brucellosis vaccine is available for human use...
  76. De Santis R, Ciammaruconi A, Faggioni G, Fillo S, Gentile B, Di Giannatale E, et al. High throughput MLVA-16 typing for Brucella based on the microfluidics technology. BMC Microbiol. 2011;11:60 pubmed publisher
    Brucellosis, a zoonosis caused by the genus Brucella, has been eradicated in Northern Europe, Australia, the USA and Canada, but remains endemic in most areas of the world...
  77. Hanna N, Jiménez de Bagüés M, Ouahrani Bettache S, El Yakhlifi Z, Kohler S, Occhialini A. The virB operon is essential for lethality of Brucella microti in the Balb/c murine model of infection. J Infect Dis. 2011;203:1129-35 pubmed publisher
    In murine infections, Brucella microti exhibits an atypical and highly pathogenic behavior resulting in a mortality of 82%...
  78. Bowman D. Introduction to the alpha-proteobacteria: Wolbachia and Bartonella, Rickettsia, Brucella, Ehrlichia, and Anaplasma. Top Companion Anim Med. 2011;26:173-7 pubmed publisher
    ..Recent work on the treatment of heartworms in dogs with doxycycline stems from related work with the human filarial nematode Onchocerca volvulus that causes river blindness in people...
  79. Binnicker M, Theel E, Larsen S, Patel R. A high percentage of serum samples that test reactive by enzyme immunoassay for anti-Brucella antibodies are not confirmed by the standard tube agglutination test. Clin Vaccine Immunol. 2012;19:1332-4 pubmed publisher
    We describe a retrospective analysis of Brucella enzyme immunoassay (EIA) IgM and IgG results compared to those of the standard tube agglutination test (SAT). Among 1,091 samples tested, 104 (9.5%) and 24 (2...
  80. Zygmunt M, Jacques I, Bernardet N, Cloeckaert A. Lipopolysaccharide heterogeneity in the atypical group of novel emerging Brucella species. Clin Vaccine Immunol. 2012;19:1370-3 pubmed publisher
    Recently, novel Brucella strains with phenotypic characteristics that were atypical for strains belonging to the genus Brucella have been reported. Phenotypically many of these strains were initially misidentified as Ochrobactrum spp...
  81. Guzmán Verri C, González Barrientos R, Hernández Mora G, Morales J, Baquero Calvo E, Chaves Olarte E, et al. Brucella ceti and brucellosis in cetaceans. Front Cell Infect Microbiol. 2012;2:3 pubmed publisher
    Since the first case of brucellosis detected in a dolphin aborted fetus, an increasing number of Brucella ceti isolates has been reported in members of the two suborders of cetaceans: Mysticeti and Odontoceti...
  82. Zhong Z, Wang Y, Xu J, Chen Y, Ke Y, Zhou X, et al. Parallel gene loss and acquisition among strains of different Brucella species and biovars. J Microbiol. 2012;50:567-74 pubmed publisher
    The genus Brucella is divided into six species; of these, B. melitensis and B. abortus are pathogenic to humans, and B. ovis and B. neotomae are nonpathogenic to humans...
  83. Gomez G, ADAMS L, Rice Ficht A, Ficht T. Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis. Front Cell Infect Microbiol. 2013;3:17 pubmed publisher
    ..The availability of genomic sequences for pathogenic Brucella spp...
  84. Cirl C, Wieser A, Yadav M, Duerr S, Schubert S, Fischer H, et al. Subversion of Toll-like receptor signaling by a unique family of bacterial Toll/interleukin-1 receptor domain-containing proteins. Nat Med. 2008;14:399-406 pubmed publisher
    ..Genes encoding TIR domain containing-proteins (Tcps) were identified in Escherichia coli CFT073 (TcpC) and Brucella melitensis (TcpB). We found that TcpC is common in the most virulent uropathogenic E...
  85. Baddour M, Alkhalifa D. Evaluation of three polymerase chain reaction techniques for detection of Brucella DNA in peripheral human blood. Can J Microbiol. 2008;54:352-7 pubmed publisher
    ..Polymerase chain reaction (PCR) has been used to detect DNA from Brucella. Different target genes, primer pairs, PCR techniques, and extraction procedures have previously been published ..
  86. Gorvel J. Brucella: a Mr "Hide" converted into Dr Jekyll. Microbes Infect. 2008;10:1010-3 pubmed publisher
    ..the causative agent of brucellosis as a small Gram-negative alpha-Proteobacterium, which was later on called Brucella melitensis in his honor by Meyer and Shaw. Nowadays, four strains exhibit pathogenicity in humans with B...
  87. Samaha H, Al Rowaily M, Khoudair R, Ashour H. Multicenter study of brucellosis in Egypt. Emerg Infect Dis. 2008;14:1916-8 pubmed publisher
    ..Examination of milk and tissues from animals in Egypt for Brucella spp. showed increased prevalence rates of serologically reactive animals. All isolates were B...
  88. Daglioglu E, Bayazit N, Okay O, Dalgic A, Hatipoglu H, Ergungor F. Lumbar epidural abscess caused by brucella species: report of two cases. Neurocirugia (Astur). 2009;20:159-62 pubmed
    Spinal epidural abscess due to Brucella species is usually associated with spondylodiscitis. Urgent surgical decompression should be performed in cases with moderate to severe neurological deficits particularly if progressive...
  89. Roop R, Gaines J, Anderson E, Caswell C, Martin D. Survival of the fittest: how Brucella strains adapt to their intracellular niche in the host. Med Microbiol Immunol. 2009;198:221-38 pubmed publisher
    b>Brucella strains produce abortion and infertility in their natural hosts and a zoonotic disease in humans known as undulant fever...
  90. de Jong M, Rolán H, Tsolis R. Innate immune encounters of the (Type) 4th kind: Brucella. Cell Microbiol. 2010;12:1195-202 pubmed publisher
    In humans, pathogenic Brucella species cause a febrile illness known as brucellosis...
  91. Audic S, Lescot M, Claverie J, Cloeckaert A, Zygmunt M. The genome sequence of Brucella pinnipedialis B2/94 sheds light on the evolutionary history of the genus Brucella. BMC Evol Biol. 2011;11:200 pubmed publisher
    Since the discovery of the Malta fever agent, Brucella melitensis, in the 19th century, six terrestrial mammal-associated Brucella species were recognized over the next century...
  92. Rinaldi J, Gallo M, Klinke S, Paris G, Bonomi H, Bogomolni R, et al. The ?-scaffold of the LOV domain of the Brucella light-activated histidine kinase is a key element for signal transduction. J Mol Biol. 2012;420:112-27 pubmed publisher
    ..Light modulates the virulence of the pathogenic bacteria Brucella abortus through LOV-HK...