acidithiobacillus thiooxidans


Summary: A strictly autotrophic species of bacteria that oxidizes sulfur and thiosulfate to sulfuric acid. It was formerly called Thiobacillus thiooxidans.

Top Publications

  1. Wang J, Bai J, Xu J, Liang B. Bioleaching of metals from printed wire boards by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans and their mixture. J Hazard Mater. 2009;172:1100-5 pubmed publisher
    ..The bacteria Acidithiobacillus ferrooxidans (A. ferrooxidans) and Acidithiobacillus thiooxidans (A...
  2. Valdes J, Ossandon F, Quatrini R, Dopson M, Holmes D. Draft genome sequence of the extremely acidophilic biomining bacterium Acidithiobacillus thiooxidans ATCC 19377 provides insights into the evolution of the Acidithiobacillus genus. J Bacteriol. 2011;193:7003-4 pubmed publisher
    b>Acidithiobacillus thiooxidans is a mesophilic, extremely acidophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and inorganic sulfur compounds...
  3. Lee E, Lee N, Cho K, Ryu H. Removal of hydrogen sulfide by sulfate-resistant Acidithiobacillus thiooxidans AZ11. J Biosci Bioeng. 2006;101:309-14 pubmed
    ..We examined three strains of Acidithiobacillus thiooxidans known to have sulfur-oxidizing activity, and identified strain AZ11 as having the highest tolerance ..
  4. Ni Y, Yang Y, Bao J, He K, Li H. Inter- and intraspecific genomic variability of the 16S-23S intergenic spacer regions (ISR) in representatives of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans. FEMS Microbiol Lett. 2007;270:58-66 pubmed
    ..The total length of the 16S-23S rRNA intergenic spacer regions of the Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans strains ranged from 451 to 490 bp, and from 434 to 456 bp, ..
  5. Lee E, Cho K, Ryu H. Simultaneous removal of H2S and NH3 in biofilter inoculated with Acidithiobacillus thiooxidans TAS. J Biosci Bioeng. 2005;99:611-5 pubmed
    ..tolerance to high concentrations of (NH4)2SO4 was isolated from activated sludge and identified as Acidithiobacillus thiooxidans TAS. A. thiooxidans TAS could display its sulfur-oxidizing activity in a medium supplemented with 60 g...
  6. Haile T, Nakhla G. The inhibitory effect of antimicrobial zeolite on the biofilm of Acidithiobacillus thiooxidans. Biodegradation. 2010;21:123-34 pubmed publisher
    The inhibitory effect of antimicrobial zeolite coated concrete specimens (Z2) against Acidithiobacillus thiooxidans was studied by measuring biomass dry cell weight (DCW), biological sulphate generation, and oxygen uptake rates (OURs)...
  7. Liu Y, Yin H, Zeng W, Liang Y, Liu Y, Baba N, et al. The effect of the introduction of exogenous strain Acidithiobacillus thiooxidans A01 on functional gene expression, structure and function of indigenous consortium during pyrite bioleaching. Bioresour Technol. 2011;102:8092-8 pubmed publisher
    b>Acidithiobacillus thiooxidans A01 was added to a consortium of bioleaching bacteria including Acidithiobacilluscaldus, Leptospirillumferriphilum, Acidithiobacillus ferrooxidans, Sulfobacillus thermosulfidooxidans, Acidiphilium spp...
  8. Lara R, García Meza J, Cruz R, Valdez Perez D, Gonzalez I. Influence of the sulfur species reactivity on biofilm conformation during pyrite colonization by Acidithiobacillus thiooxidans. Appl Microbiol Biotechnol. 2012;95:799-809 pubmed publisher
    ..Selected modified pyrite surfaces were incubated with cells of sulfur-oxidizing Acidithiobacillus thiooxidans for 24 h in a specific culture medium (pH 2)...
  9. Bobadilla Fazzini R, Cortés M, Padilla L, Maturana D, Budinich M, Maass A, et al. Stoichiometric modeling of oxidation of reduced inorganic sulfur compounds (Riscs) in Acidithiobacillus thiooxidans. Biotechnol Bioeng. 2013;110:2242-51 pubmed publisher
    ..first experimentally validated stoichiometric model able to assess RISCs oxidation quantitatively in Acidithiobacillus thiooxidans (strain DSM 17318), the archetype of the sulfur oxidizing acidophilic chemolithoautotrophs...

More Information


  1. Gallegos M, Falco L, Peluso M, Sambeth J, Thomas H. Recovery of manganese oxides from spent alkaline and zinc-carbon batteries. An application as catalysts for VOCs elimination. Waste Manag. 2013;33:1483-90 pubmed publisher
    ..a pilot plant consisting in: an air-lift bioreactor (containing an acid-reducing medium produced by an Acidithiobacillus thiooxidans bacteria immobilized on elemental sulfur); a leaching reactor (were battery powder is mixed with the ..
  2. Ren W, Li P, Zheng L, Fan S, Verhozina V. Effects of dissolved low molecular weight organic acids on oxidation of ferrous iron by Acidithiobacillus ferrooxidans. J Hazard Mater. 2009;162:17-22 pubmed publisher
    ..Hence, it is feasible that R2 might be used for bioleaching of soils contaminated with metals or metals coupled with organic compounds because of the higher concentrations of LMW organic acids to which R2 is tolerant...
  3. He Z, Zhong H, Hu Y, Xiao S, Liu J, Xu J, et al. Analysis of differential-expressed proteins of Acidithiobacillus ferrooxidans grown under phosphate starvation. J Biochem Mol Biol. 2005;38:545-9 pubmed
    ..From the 6 identified protein spots, 3 proteins were found to be decreased in expression at the cultivating condition of phosphate starvation, while another three upregulated. ..
  4. Janosch C, Remonsellez F, Sand W, Vera M. Sulfur Oxygenase Reductase (Sor) in the Moderately Thermoacidophilic Leaching Bacteria: Studies in Sulfobacillus thermosulfidooxidans and Acidithiobacillus caldus. Microorganisms. 2015;3:707-24 pubmed publisher also encoded in the genomes of some acidophilic leaching bacteria such as Acidithiobacillus caldus, Acidithiobacillus thiooxidans, Acidithiobacillus ferrivorans and Sulfobacillus thermosulfidooxidans, among others...
  5. Kim D, Mishra D, Ahn J, Chaudhury G, Ralph D. A novel process to treat spent petroleum catalyst using sulfur-oxidizing lithotrophs. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009;44:1585-91 pubmed publisher
    ..The leaching kinetics followed 1st order reaction rate. Finally, multiple linear regression analysis was carried out to compare the observed and calculated leaching percentage values for three metals. ..
  6. Díaz M, Castro M, Copaja S, Guiliani N. Biofilm Formation by the Acidophile Bacterium Acidithiobacillus thiooxidans Involves c-di-GMP Pathway and Pel exopolysaccharide. Genes (Basel). 2018;9: pubmed publisher
  7. Cwalina B, Dec W, Michalska J, Jaworska Kik M, Student S. Initial stage of the biofilm formation on the NiTi and Ti6Al4V surface by the sulphur-oxidizing bacteria and sulphate-reducing bacteria. J Mater Sci Mater Med. 2017;28:173 pubmed publisher
    ..The biofilms formed on alloy surfaces by the cells of five bacterial strains (aerobic SOB Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans, and anaerobic SRB Desulfovibrio desulfuricans-3 strains) were ..
  8. Soleimani S, Ormeci B, Isgor O. Evaluation of E. coli biofilm as a protective barrier against microbiologically influenced deterioration of concrete (MICD) under mesophilic temperatures. Water Sci Technol. 2013;68:303-10 pubmed publisher
    ..Overall, the results indicate that the E. coli DH5? biofilm showed good protection against MICD induced by SOB at 37 °C. ..
  9. Nugroho R, Takanashi H, Hirata M, Hano T. Denitrification of industrial wastewater with sulfur and limestone packed column. Water Sci Technol. 2002;46:99-104 pubmed
    ..The wastewater from steel production plants was treated by the present biofilm process. Low extent of nitrogen removal was caused by the lack of minerals. ..
  10. Ramírez Aldaba H, Valles O, Vazquez Arenas J, Rojas Contreras J, Valdez Pérez D, Ruiz Baca E, et al. Chemical and surface analysis during evolution of arsenopyrite oxidation by Acidithiobacillus thiooxidans in the presence and absence of supplementary arsenic. Sci Total Environ. 2016;566-567:1106-1119 pubmed publisher
    ..The current study aims to evaluate the arsenopyrite oxidation by Acidithiobacillus thiooxidans during 240h at different time intervals, in the presence and absence of supplementary arsenic...
  11. Valdés F, Camiloti P, Rodriguez R, Delforno T, Carrillo Reyes J, Zaiat M, et al. Sulfide-oxidizing bacteria establishment in an innovative microaerobic reactor with an internal silicone membrane for sulfur recovery from wastewater. Biodegradation. 2016;27:119-30 pubmed publisher
    ..Pyrosequencing analysis indicated various sulfide-oxidizing bacteria (SOB) affiliated to the species Acidithiobacillus thiooxidans, Sulfuricurvum kujiense and Pseudomonas stutzeri attached to the membrane and also indicated ..
  12. Zeng J, Gou M, Tang Y, Li G, Sun Z, Kida K. Effective bioleaching of chromium in tannery sludge with an enriched sulfur-oxidizing bacterial community. Bioresour Technol. 2016;218:859-66 pubmed publisher
    ..9-97.9% were observed for chromium in oxidizable and residual fractions. Acidithiobacillus thiooxidans, the predominant bacteria in the enriched community, played an important role in bioleaching, whereas ..
  13. Işıldar A, van de Vossenberg J, Rene E, van Hullebusch E, Lens P. Two-step bioleaching of copper and gold from discarded printed circuit boards (PCB). Waste Manag. 2016;57:149-157 pubmed publisher
    ..In the first step, chemolithotrophic acidophilic Acidithiobacillus ferrivorans and Acidithiobacillus thiooxidans were used...
  14. Knight J, Cheeseman C, Rogers R. Microbial influenced degradation of solidified waste binder. Waste Manag. 2002;22:187-93 pubmed
    ..It was not possible to distinguish differences in corrosion due to the chemical and microbial influenced degradation from the results obtained to date and further work is focusing on modified procedures to address this. ..
  15. Zhou Q, Gao J, Li Y, Zhu S, He L, Nie W, et al. Bioleaching in batch tests for improving sludge dewaterability and metal removal using Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans after cold acclimation. Water Sci Technol. 2017;76:1347-1359 pubmed publisher
    ..In this study, Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans were acclimated at 15 °C and co-inoculated to explore the optimal conditions for improvement of ..
  16. Latorre M, Cortés M, Travisany D, Di Genova A, Budinich M, Reyes Jara A, et al. The bioleaching potential of a bacterial consortium. Bioresour Technol. 2016;218:659-66 pubmed publisher
    ..The strains Acidithiobacillus thiooxidans Licanantay, Acidiphilium multivorum Yenapatur, Leptospirillum ferriphilum Pañiwe, Acidithiobacillus ..
  17. Naresh Kumar R, Nagendran R. Fractionation behavior of heavy metals in soil during bioleaching with Acidithiobacillus thiooxidans. J Hazard Mater. 2009;169:1119-26 pubmed publisher
    ..Acidophilic sulfur oxidizing bacteria Acidithiobacillus thiooxidans isolated from soil was used for bioleaching...
  18. Takeuchi T, Suzuki I. Effect of pH on sulfite oxidation by Thiobacillus thiooxidans cells with sulfurous acid or sulfur dioxide as a possible substrate. J Bacteriol. 1994;176:913-6 pubmed
    ..The results from kinetic studies show that the fully protonated form of sulfite, sulfurous acid or sulfur dioxide, is the form which penetrates the cells for the oxidation...
  19. Dominy C, Deane S, Rawlings D. A geographically widespread plasmid from Thiobacillus ferrooxidans has genes for ferredoxin-, FNR-, prismane- and NADH-oxidoreductase-like proteins which are also located on the chromosome. Microbiology. 1997;143 ( Pt 10):3123-36 pubmed
    ..This region contained no ORFs and was identical to that previously reported for the replicon of a 9.8 kb T. ferrooxidans plasmid, pTF191...
  20. Sağlam E, Akçay M, Colak D, İnan Bektaş K, Belduz A. Generation of acid mine drainage around the Karaerik copper mine (Espiye, Giresun, NE Turkey): implications from the bacterial population in the Ac?su effluent. Extremophiles. 2016;20:673-85 pubmed publisher iron-reducing bacteria, and Acidithiobacillus ferrivorans, Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Acidiphilium cryptum as sulphur-oxidising bacteria...
  21. Marra A, Cesaro A, Rene E, Belgiorno V, Lens P. Bioleaching of metals from WEEE shredding dust. J Environ Manage. 2018;210:180-190 pubmed publisher
    ..In the first step, base metals were almost completely leached from the dust in 8 days by Acidithiobacillus thiooxidans (DSM 9463) that lowered the pH of the leaching solution from 3.5 to 1.0...
  22. Hao L, Liu X, Wang H, Lin J, Pang X, Lin J. Detection and validation of a small broad-host-range plasmid pBBR1MCS-2 for use in genetic manipulation of the extremely acidophilic Acidithiobacillus sp. J Microbiol Methods. 2012;90:309-14 pubmed publisher
    ..Our results indicate that pBBR1MCS-2 provides a new and useful tool in the genetic manipulation of Acidithiobacillus strains. ..
  23. Matlakowska R, Sklodowska A. Adaptive changes of chemolithoautotrophic acidophilic sulfur-oxidizing bacteria during growth in sewage sludge. Can J Microbiol. 2006;52:1189-98 pubmed
    ..acidophilic, sulfur-oxidizing strain was isolated from sewage sludge and identified as Acidithiobacillus thiooxidans. The morphology and physiology of the isolate grown in mineral medium or sterilized sewage sludge were ..
  24. Belyi A, Pustoshilov P, Gurevich I, Kadochnikova G, Ladygina V. [Bacterial leaching of manganese ores]. Prikl Biokhim Mikrobiol. 2006;42:327-31 pubmed
    ..Inoculation of bacteria into pulp with pyrite increased the rate of leaching of oxide ore (psilomelane) by 37%, and the degree of its extraction within 180 h increased from 80 to 97%. ..
  25. Nguyen T, Fu C, Juang R. Biosorption and biodegradation of a sulfur dye in high-strength dyeing wastewater by Acidithiobacillus thiooxidans. J Environ Manage. 2016;182:265-271 pubmed publisher
    The ability of the bacterial strain Acidithiobacillus thiooxidans to remove sulfur blue 15 (SB15) dye from water samples was examined...
  26. Qin J, Fu H, Ye J, Bencze K, Stemmler T, Rawlings D, et al. Convergent evolution of a new arsenic binding site in the ArsR/SmtB family of metalloregulators. J Biol Chem. 2007;282:34346-55 pubmed
    ..These results suggest that the As(III)-S(3) binding sites in AfArsR and R773 ArsR arose independently at spatially distinct locations in their three-dimensional structures. ..
  27. Lombardi A, Garcia Júnior O, Mozeto A. Bioleaching of metals from anaerobic sewage sludge: effects of total solids, leaching microorganisms, and energy source. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2001;36:793-806 pubmed
    ..It was also demonstrated that if lead (Pb) solubilization is to be achieved, than a special combination of microorganism/energy source must be applied. ..
  28. Ko M, Park H, Kim K, Lee J. The role of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in arsenic bioleaching from soil. Environ Geochem Health. 2013;35:727-33 pubmed publisher
    ..As from the soil in an abandoned Ag-Au mine was carried out using Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. A. ferrooxidans is an iron oxidizer and A. thiooxidans is a sulfur oxidizer...
  29. Nareshkumar R, Nagendran R. Changes in nutrient profile of soil subjected to bioleaching for removal of heavy metals using Acidithiobacillus thiooxidans. J Hazard Mater. 2008;156:102-7 pubmed publisher
    ..Indigenous sulfur oxidizing bacteria Acidithiobacillus thiooxidans isolated from the contaminated soil were used for bioremediation...
  30. Nakamura K, Nakamura M, Yoshikawa H, Amano Y. Purification and properties of thiosulfate dehydrogenase from Acidithiobacillus thiooxidans JCM7814. Biosci Biotechnol Biochem. 2001;65:102-8 pubmed
    A key enzyme of the thiosulfate oxidation pathway in Acidithiobacillus thiooxidans JCM7814 was investigated. As a result of assaying the enzymatic activities of thiosulfate dehydrogenase, rhodanese, and thiosulfate reductase at 5...
  31. Carmalin Sophia A, Swaminathan K, Sandhya S. Microbially-influenced degradation of solidified/stabilized metal waste. Bioresour Technol. 2007;98:2562-7 pubmed
    ..Of the two approaches, the bulk diffusion model fit the data better than the acid dissolution model as indicated by the correlation coefficients of >0.97...
  32. Ramírez M, Fernández M, Granada C, Le Borgne S, Gomez J, Cantero D. Biofiltration of reduced sulphur compounds and community analysis of sulphur-oxidizing bacteria. Bioresour Technol. 2011;102:4047-53 pubmed publisher
    ..The first biofilter was inoculated with Acidithiobacillus thiooxidans (BAT) and the second one with Thiobacillus thioparus (BTT)...
  33. Liu H, Chiu C, Cheng Y. The effects of metabolites from the indigenous Acidithiobacillus thiooxidans and temperature on the bioleaching of cadmium from soil. Biotechnol Bioeng. 2003;83:638-45 pubmed
    The effect of metabolites from the indigenous Acidithiobacillus thiooxidans and temperature on the bioleaching of cadmium from soil was investigated in the present study...
  34. Sharmin S, Yoshino E, Kanao T, Kamimura K. Characterization of a novel thiosulfate dehydrogenase from a marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH. Biosci Biotechnol Biochem. 2016;80:273-8 pubmed publisher
    A marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH, was isolated to develop a bioleaching process for NaCl-containing sulfide minerals...
  35. Idachaba M, Nyavor K, Egiebor N, Rogers R. Stability evaluation of a cement based waste form to microbially induced degradation. Waste Manag Res. 2001;19:284-91 pubmed
    ..thiooxidans, while the use of a culture that is substrate limited could result in an underestimation of T. thiooxidans capability...
  36. Levican G, Ugalde J, Ehrenfeld N, Maass A, Parada P. Comparative genomic analysis of carbon and nitrogen assimilation mechanisms in three indigenous bioleaching bacteria: predictions and validations. BMC Genomics. 2008;9:581 pubmed publisher
    ..This study demonstrated that there are important differences in the carbon dioxide and nitrogen fixation mechanisms among bioleaching bacteria that coexist in mining environments...
  37. Negishi A, Muraoka T, Maeda T, Takeuchi F, Kanao T, Kamimura K, et al. Growth inhibition by tungsten in the sulfur-oxidizing bacterium Acidithiobacillus thiooxidans. Biosci Biotechnol Biochem. 2005;69:2073-80 pubmed
    Growth of five strains of sulfur-oxidizing bacteria Acidithiobacillus thiooxidans, including strain NB1-3, was inhibited completely by 50 microM of sodium tungstate (Na(2)WO(4)). When the cells of NB1-3 were incubated in 0...
  38. Wang Y, Pan Z, Lang J, Xu J, Zheng Y. Bioleaching of chromium from tannery sludge by indigenous Acidithiobacillus thiooxidans. J Hazard Mater. 2007;147:319-24 pubmed
    ..The acidophilic sulfur-oxidizing Acidithiobacillus thiooxidans can leach heavy metals form urban and industrial wastes...
  39. Koban A, Bernhard G. Uranium(VI) complexes with phospholipid model compounds--a laser spectroscopic study. J Inorg Biochem. 2007;101:750-7 pubmed
  40. Okabe S, Odagiri M, Ito T, Satoh H. Succession of sulfur-oxidizing bacteria in the microbial community on corroding concrete in sewer systems. Appl Environ Microbiol. 2007;73:971-80 pubmed
    ..plumbophilus, Thiomonas intermedia, Halothiobacillus neapolitanus, Acidiphilium acidophilum, and Acidithiobacillus thiooxidans. A...
  41. Rosi P, Miscoria S, Bernik D, Martín Negri R. Customized design of electronic noses placed on top of air-lift bioreactors for in situ monitoring the off-gas patterns. Bioprocess Biosyst Eng. 2012;35:835-42 pubmed publisher
    ..The device was applied to follow the off-gas of a bioreactor with Acidithiobacillus thiooxidans grown on beds of elemental sulfur under aerobic conditions...
  42. Gómez Ramírez M, Zarco Tovar K, Aburto J, de León R, Rojas Avelizapa N. Microbial treatment of sulfur-contaminated industrial wastes. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2014;49:228-32 pubmed publisher
    ..The study involved the use of two bacteria Acidithiobacillus ferrooxidans ATCC 53987 and Acidithiobacillus thiooxidans AZCT-M125-5 isolated from a Mexican soil...
  43. Kim H, Lee K, Lee B, Kim S, Kim K. Comparative study of simultaneous removal of As, Cu, and Pb using different combinations of electrokinetics with bioleaching by Acidithiobacillus ferrooxidans. Water Res. 2012;46:5591-5599 pubmed publisher
    ..A pilot-scale experiment indicated that integrated bioelectrokinetics is an effective means of remediation of soils contaminated with multiple heavy metals and arsenic. ..
  44. Zeng J, Geng M, Liu Y, Zhao W, Xia L, Liu J, et al. Expression, purification and molecular modelling of the Iro protein from Acidithiobacillus ferrooxidans Fe-1. Protein Expr Purif. 2007;52:146-52 pubmed
    ..As we know, this is the first report of expression in E. coli of the Iro protein from A. ferrooxidans Fe-1...
  45. Bobadilla Fazzini R, Levican G, Parada P. Acidithiobacillus thiooxidans secretome containing a newly described lipoprotein Licanantase enhances chalcopyrite bioleaching rate. Appl Microbiol Biotechnol. 2011;89:771-80 pubmed publisher
    ..This work characterizes for the first time the sulfur (meta)secretome of Acidithiobacillus thiooxidans strain DSM 17318 in pure and mixed cultures with Acidithiobacillus ferrooxidans DSM 16786, identifying ..
  46. Xu Y, Yin H, Jiang H, Liang Y, Guo X, Ma L, et al. Comparative study of nickel resistance of pure culture and co-culture of Acidithiobacillus thiooxidans and Leptospirillum ferriphilum. Arch Microbiol. 2013;195:637-46 pubmed publisher
    The effect of Ni²? on the growth and functional gene expression of the pure culture and co-culture of Acidithiobacillus thiooxidans and Leptospirillum ferriphilum has been studied...
  47. Smeulders M, Pol A, Venselaar H, Barends T, Hermans J, Jetten M, et al. Bacterial CS2 hydrolases from Acidithiobacillus thiooxidans strains are homologous to the archaeal catenane CS2 hydrolase. J Bacteriol. 2013;195:4046-56 pubmed publisher
    ..These compounds can be converted by sulfur-oxidizing bacteria, such as Acidithiobacillus thiooxidans species, to carbon dioxide (CO(2)) and hydrogen sulfide (H2S), a property used in industrial ..
  48. Kamimura K, Higashino E, Moriya S, Sugio T. Marine acidophilic sulfur-oxidizing bacterium requiring salts for the oxidation of reduced inorganic sulfur compounds. Extremophiles. 2003;7:95-9 pubmed
    ..Phylogenetic analysis based on 16S rDNA sequences indicated that strain SH had a close relationship to Acidithiobacillus thiooxidans. The oxidizing activities of sulfur and sulfite with resting cells were stimulated not only by the ..
  49. Liang G, Tang J, Liu W, Zhou Q. Optimizing mixed culture of two acidophiles to improve copper recovery from printed circuit boards (PCBs). J Hazard Mater. 2013;250-251:238-45 pubmed publisher
    ..of printed circuit boards (PCBs) on cells growth and metals recovery in separated and mixed culture of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans were investigated...
  50. Lara R, García Meza J, Gonzalez I, Cruz R. Influence of the surface speciation on biofilm attachment to chalcopyrite by Acidithiobacillus thiooxidans. Appl Microbiol Biotechnol. 2013;97:2711-24 pubmed publisher
    ..Modified surfaces were incubated with cells of sulfur-oxidizing bacteria (Acidithiobacillus thiooxidans) for 24 h in a specific culture medium (pH 2)...
  51. Masau R, Oh J, Suzuki I. Mechanism of oxidation of inorganic sulfur compounds by thiosulfate-grown Thiobacillus thiooxidans. Can J Microbiol. 2001;47:348-58 pubmed
  52. Behera S, Panda S, Pradhan N, Sukla L, Mishra B. Extraction of nickel by microbial reduction of lateritic chromite overburden of Sukinda, India. Bioresour Technol. 2012;125:17-22 pubmed publisher
    ..Kinetics study of anoxic microbial processing of COB revealed that the chemical reaction rate control model fits to the rate of nickel dissolution (R(2)=0.975)...
  53. Tupikina O, Kondrat eva T, Samorukova V, Rassulov V, Karavaiko G. [Dependence of the genotypic characteristics of Acidithiobacillus ferrooxidans on the physical, chemical, and electrophysical properties of pyrites]. Mikrobiologiia. 2005;74:596-603 pubmed
  54. Liu F, Zhou J, Wang D, Zhou L. Enhancing sewage sludge dewaterability by bioleaching approach with comparison to other physical and chemical conditioning methods. J Environ Sci (China). 2012;24:1403-10 pubmed
    ..The conditioning result of bioleaching by Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans on sludge dewatering was investigated and compared with the effects ..
  55. Kumar R, Nagendran R. Influence of initial pH on bioleaching of heavy metals from contaminated soil employing indigenous Acidithiobacillus thiooxidans. Chemosphere. 2007;66:1775-81 pubmed
    ..of heavy metals from contaminated soil was carried out employing indigenous sulfur oxidizing bacterium Acidithiobacillus thiooxidans. Experiments were carried out to assess the influence of initial pH of the system on bioleaching of ..
  56. Zhu J, Li Q, Jiao W, Jiang H, Sand W, Xia J, et al. Adhesion forces between cells of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans or Leptospirillum ferrooxidans and chalcopyrite. Colloids Surf B Biointerfaces. 2012;94:95-100 pubmed publisher
    ..The adhesion of three species of leaching microorganisms including Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans to chalcopyrite was investigated by using atomic force microscopy (AFM)..
  57. Chen P, Yan L, Leng F, Nan W, Yue X, Zheng Y, et al. Bioleaching of realgar by Acidithiobacillus ferrooxidans using ferrous iron and elemental sulfur as the sole and mixed energy sources. Bioresour Technol. 2011;102:3260-7 pubmed publisher
  58. Ishigaki T, Nakanishi A, Tateda M, Ike M, Fujita M. Bioleaching of metal from municipal waste incineration fly ash using a mixed culture of sulfur-oxidizing and iron-oxidizing bacteria. Chemosphere. 2005;60:1087-94 pubmed
    ..These results suggested that bioleaching using a mixed culture of SOB and IOB is a promising technology for recovering the valuable metals from MSWI fly ash...
  59. Sugio T, Hisazumi T, Kanao T, Kamimura K, Takeuchi F, Negishi A. Existence of aa3-type ubiquinol oxidase as a terminal oxidase in sulfite oxidation of Acidithiobacillus thiooxidans. Biosci Biotechnol Biochem. 2006;70:1584-91 pubmed
    It was found that Acidithiobacillus thiooxidans has sulfite:ubiquinone oxidoreductase and ubiquinol oxidase activities in the cells. Ubiquinol oxidase was purified from plasma membranes of strain NB1-3 in a nearly homogeneous state...
  60. Bredberg K, Karlsson H, Holst O. Reduction of vanadium(V) with Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. Bioresour Technol. 2004;92:93-6 pubmed
    ..A. ferrooxidans was compared with Acidithiobacillus thiooxidans, which has previously been utilized for vanadium leaching and reduction...
  61. Duan H, Koe L, Yan R. Treatment of H2S using a horizontal biotrickling filter based on biological activated carbon: reactor setup and performance evaluation. Appl Microbiol Biotechnol. 2005;67:143-9 pubmed
    ..A mixed culture of sulfide-oxidizing bacteria dominated by Acidithiobacillus thiooxidans acclimated from activated sludge was used as bacterial seed and the biofilm was developed by culturing ..
  62. Narayanan R, Sreekrishnan T. A two-stage process for simultaneous thermophilic sludge digestion, pathogen control and metal leaching. Environ Technol. 2009;30:21-6 pubmed publisher
    ..Studies with 1.5 L reactors indicate that at the end of the process, concentration of volatile suspended solids has stabilized, no coliforms are present, and 90-98% copper, 90-94% manganese and 95-100% zinc has been leached...
  63. Sercu B, Nunez D, Van Langenhove H, Aroca G, Verstraete W. Operational and microbiological aspects of a bioaugmented two-stage biotrickling filter removing hydrogen sulfide and dimethyl sulfide. Biotechnol Bioeng. 2005;90:259-69 pubmed
    ..The first biotrickling filter (ABF) was inoculated with Acidithiobacillus thiooxidans and operated without pH control, while the second biotrickling filter (HBF) was inoculated with ..
  64. Idachaba M, Nyavor K, Egiebor N, Rogers R. A refinement of the biofilm formation method for waste forms stability evaluation. J Hazard Mater. 2001;84:95-106 pubmed
  65. Kondrat eva T, Danilevich V, Ageeva S, Karavaiko G. Identification of IS elements in Acidithiobacillus ferrooxidans strains grown in a medium with ferrous iron or adapted to elemental sulfur. Arch Microbiol. 2005;183:401-10 pubmed
    ..ferrooxidans strains caused changes in the number, intensity and localization of hybridization bands. The authors discuss the role of IS elements in the adaptation of A. ferrooxidans to the new energy substrate...
  66. Zhang J, Wei W, Mao Y, Chai L. Monitoring of bio-oxidation process of ferrous ion by using piezoelectric impedance analysis. Curr Microbiol. 2001;43:83-8 pubmed
    ..The results showed that the proposed method could provide real time and multidimensional information to monitoring of the bio-oxidation process...
  67. Harrington C, Vidler D, Watts M, Hall J. Potential for using isotopically altered metalloproteins in species-specific isotope dilution analysis of proteins by HPLC coupled to inductively coupled plasma mass spectrometry. Anal Chem. 2005;77:4034-41 pubmed
    ..No exchange of the enriched copper isotope from Rc with an added naturally abundant inorganic copper cation was observed under a neutral pH environment, indicating that species-specific ID-MS analysis of metalloproteins is possible...
  68. Suzuki I, Lee D, Mackay B, Harahuc L, Oh J. Effect of various ions, pH, and osmotic pressure on oxidation of elemental sulfur by Thiobacillus thiooxidans. Appl Environ Microbiol. 1999;65:5163-8 pubmed
    ..The results agree with the expected response of this acidophilic bacterium to charge neutralization of colloids by ions, pH-dependent membrane permeability of ions, and osmotic pressure...
  69. Tan G, Shu W, Hallberg K, Li F, Lan C, Zhou W, et al. Culturable and molecular phylogenetic diversity of microorganisms in an open-dumped, extremely acidic Pb/Zn mine tailings. Extremophiles. 2008;12:657-64 pubmed publisher
  70. Ramirez M, Gomez J, Cantero D, Paca J, Halecky M, Kozliak E, et al. Hydrogen sulfide removal from air by Acidithiobacillus thiooxidans in a trickle bed reactor. Folia Microbiol (Praha). 2009;54:409-14 pubmed publisher
    A strain of Acidithiobacillus thiooxidans immobilized in polyurethane foam was utilized for H(2)S removal in a bench-scale trickle-bed reactor, testing the limits of acidity and SO(4) (2-) accumulation...
  71. Zhu J, Jiao W, Li Q, Liu X, Qin W, Qiu G, et al. Investigation of energy gene expressions and community structures of free and attached acidophilic bacteria in chalcopyrite bioleaching. J Ind Microbiol Biotechnol. 2012;39:1833-40 pubmed publisher
    ..This conclusion was supported by the results of HPLC analysis. Sulfur-oxidizing Acidithiobacillus thiooxidans and ferrous-oxidizing Leptospirillum ferrooxidans were the members of the mixed culture in ..
  72. Lee K, Kim K. Heavy metal removal from shooting range soil by hybrid electrokinetics with bacteria and enhancing agents. Environ Sci Technol. 2010;44:9482-7 pubmed publisher
    ..Active bioaugmentation was performed using Acidithiobacillus thiooxidans in the bioleaching step, and each test was sequentially combined with acid-enhanced and EDTA-enhanced ..
  73. Jones D, Albrecht H, Dawson K, Schaperdoth I, Freeman K, Pi Y, et al. Community genomic analysis of an extremely acidophilic sulfur-oxidizing biofilm. ISME J. 2012;6:158-70 pubmed publisher
    ..Snottites from the Frasassi cave system (Italy) are dominated (>70% of cells) by Acidithiobacillus thiooxidans, with smaller populations including an archaeon in the uncultivated 'G-plasma' clade of ..
  74. Lee K, Kim H, Lee B, Kim S, Kwon Y, Kim K. A feasibility study on bioelectrokinetics for the removal of heavy metals from tailing soil. Environ Geochem Health. 2011;33 Suppl 1:3-11 pubmed publisher
    ..In this study, the bioleaching process driven by injection of S-oxidizing bacteria, Acidithiobacillus thiooxidans, was evaluated as a pre-treatment step...
  75. Tian K, Lin J, Liu X, Liu Y, Zhang C. [Expression of phosphofructokinase gene from Escherichia coli K-12 in obligately autotrophic bacterium Acidithiobacillus thiooxidans]. Wei Sheng Wu Xue Bao. 2003;43:592-8 pubmed
    ..7.1. 11) gene (pfkA) was constructed and transferred into Acidithiobacillus thiooxidans Tt-Z2 by conjugation. The transfer frequency of plasmid from E. coli to Tt-Z2 was 2.6 x 10(-6)...
  76. Kamimura K, Higashino E, Kanao T, Sugio T. Effects of inhibitors and NaCl on the oxidation of reduced inorganic sulfur compounds by a marine acidophilic, sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH. Extremophiles. 2005;9:45-51 pubmed
    ..of reduced inorganic sulfur compounds were studied using resting cells and cell-free extracts of Acidithiobacillus thiooxidans strain SH. This isolate specifically requires NaCl for growth...
  77. Butcher B, Deane S, Rawlings D. The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli. Appl Environ Microbiol. 2000;66:1826-33 pubmed
    ..This divergent orientation was conserved in the four T. ferrooxidans strains investigated...
  78. Starosvetsky J, Zukerman U, Armon R. A simple medium modification for isolation, growth and enumeration of Acidithiobacillus thiooxidans (syn. Thiobacillus thiooxidans) from water samples. J Microbiol Methods. 2013;92:178-82 pubmed publisher
    ..This study revealed that BCG modified Thiobacillus agar is substantially time saving and much easier to infer compared to MPN technique...
  79. Mishra D, Kim D, Ralph D, Ahn J, Rhee Y. Bioleaching of spent hydro-processing catalyst using acidophilic bacteria and its kinetics aspect. J Hazard Mater. 2008;152:1082-91 pubmed
    ..During the whole process Mo showed low dissolution because of substantiate precipitation with leach residues as MoO3. Bioleach residues were characterized by EDX and XRD...
  80. García Meza J, Fernandez J, Lara R, Gonzalez I. Changes in biofilm structure during the colonization of chalcopyrite by Acidithiobacillus thiooxidans. Appl Microbiol Biotechnol. 2013;97:6065-75 pubmed publisher
    Biofilms of Acidithiobacillus thiooxidans were grown on the surface of massive chalcopyrite electrodes (MCE) where different secondary sulfur phases were previously formed by potentiostatic oxidation of MCE at 0.780?Ean?0...