pectobacterium chrysanthemi

Summary

Summary: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that causes vascular wilts on a wide range of plant species. It was formerly named Erwinia chrysanthemi.

Top Publications

  1. Franza T, Michaud Soret I, Piquerel P, Expert D. Coupling of iron assimilation and pectinolysis in Erwinia chrysanthemi 3937. Mol Plant Microbe Interact. 2002;15:1181-91 pubmed
    ..These important pathogenicity functions are coregulated by responding to common signals encountered in planta...
  2. Samson R, Legendre J, Christen R, Fischer Le Saux M, Achouak W, Gardan L. Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species,. Int J Syst Evol Microbiol. 2005;55:1415-27 pubmed
    A collection of 75 strains of Pectobacterium chrysanthemi (including all biovars and pathovars) and the type strains of Brenneria paradisiaca (CFBP 4178(T)) and Pectobacterium cypripedii (CFBP 3613(T)) were studied by DNA-DNA ..
  3. Hilf R, Dutzler R. X-ray structure of a prokaryotic pentameric ligand-gated ion channel. Nature. 2008;452:375-9 pubmed publisher
    ..3 A resolution. Our study reveals the first structure of a pLGIC at high resolution and provides an important model system for the investigation of the general mechanisms of ion permeation and gating within the family...
  4. Cheng X, Ivanov I, Wang H, Sine S, McCammon J. Molecular-dynamics simulations of ELIC-a prokaryotic homologue of the nicotinic acetylcholine receptor. Biophys J. 2009;96:4502-13 pubmed publisher
    ..Finally, the simulation of an ELIC mutant substantiates the important role of F246 on the stability, hydration and possibly function of the ELIC channel...
  5. Gonzalez Gutierrez G, Lukk T, Agarwal V, Papke D, Nair S, Grosman C. Mutations that stabilize the open state of the Erwinia chrisanthemi ligand-gated ion channel fail to change the conformation of the pore domain in crystals. Proc Natl Acad Sci U S A. 2012;109:6331-6 pubmed publisher
    ..Overall, our findings bring to light the limited power of functional studies in intact membranes when it comes to inferring the functional state of a channel in a crystal, at least in the case of the nicotinic-receptor superfamily...
  6. Delangle A, Prouvost A, Cogez V, Bohin J, Lacroix J, Cotte Pattat N. Characterization of the Erwinia chrysanthemi Gan locus, involved in galactan catabolism. J Bacteriol. 2007;189:7053-61 pubmed
    ..These mutations did not affect the virulence of E. chrysanthemi on chicory leaves, potato tubers, or Saintpaulia ionantha, suggesting an accessory role of galactan utilization in the bacterial pathogeny...
  7. Hilf R, Dutzler R. Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel. Nature. 2009;457:115-8 pubmed publisher
    ..In combination, both structures suggest a novel gating mechanism for pentameric ligand-gated ion channels where channel opening proceeds by a change in the tilt of the pore-forming helices...
  8. Blot N, Berrier C, Hugouvieux Cotte Pattat N, Ghazi A, Condemine G. The oligogalacturonate-specific porin KdgM of Erwinia chrysanthemi belongs to a new porin family. J Biol Chem. 2002;277:7936-44 pubmed publisher
    ..KdgM has no homology with currently known porins, but proteins similar to KdgM are present in several bacteria. Therefore, these proteins might constitute a new family of porin channels...
  9. Grenier A, Duport G, Pages S, Condemine G, Rahbe Y. The phytopathogen Dickeya dadantii (Erwinia chrysanthemi 3937) is a pathogen of the pea aphid. Appl Environ Microbiol. 2006;72:1956-65 pubmed
    ..The observed difference in virulence toward apterous and winged aphids may have an ecological impact, and this deserves specific attention in future research...

More Information

Publications97

  1. Franza T, Mahé B, Expert D. Erwinia chrysanthemi requires a second iron transport route dependent of the siderophore achromobactin for extracellular growth and plant infection. Mol Microbiol. 2005;55:261-75 pubmed
    ..Interestingly, iron transport mediated by achromobactin or a closely related siderophore probably exists in other phytopathogenic bacterial species such as Pseudomonas syringae...
  2. Kotzia G, Labrou N. L-Asparaginase from Erwinia Chrysanthemi 3937: cloning, expression and characterization. J Biotechnol. 2007;127:657-69 pubmed
    ..The approach offers the possibility of designing an ErL-ASNase bioreactor that can be operated over a long period of time with high efficiency, which can be used in leukaemia therapy...
  3. Lim W, Hong S, An C, Cho K, Choi B, Kim Y, et al. Construction of minimum size cellulase (Cel5Z) from Pectobacterium chrysanthemi PY35 by removal of the C-terminal region. Appl Microbiol Biotechnol. 2005;68:46-52 pubmed
    b>Pectobacterium chrysanthemi PY35 secretes the endoglucanase Cel5Z, an enzyme of the glycoside hydrolase family 5...
  4. Park S, Cho S, Kim M, Ryu S, Lim W, An C, et al. Activity enhancement of Cel5Z from Pectobacterium chrysanthemi PY35 by removing C-terminal region. Biochem Biophys Res Commun. 2002;291:425-30 pubmed
    ..coli transformant appeared to be 32 kDa by SDS-PAGE analysis in the presence of carboxymethyl-cellulose (CMC). The Cel5Z::Omega protein hydrolyzed CMC with 1.7-fold higher activity than the intact Cel5Z cellulase...
  5. Cho K, Lim W, Math R, Asraful Islam S, Hong S, Kim H, et al. Comparative analysis of the glg operons of Pectobacterium chrysanthemi PY35 and other prokaryotes. J Mol Evol. 2008;67:1-12 pubmed publisher
    A chromosomal region of Pectobacterium chrysanthemi PY35 that contains of genes for glycogen synthesis was isolated from a cosmid library...
  6. Bocquet N, Nury H, Baaden M, Le Poupon C, Changeux J, Delarue M, et al. X-ray structure of a pentameric ligand-gated ion channel in an apparently open conformation. Nature. 2009;457:111-4 pubmed publisher
    ..These data are consistent with a model of pore opening based on both quaternary twist and tertiary deformation...
  7. Zimmermann I, Dutzler R. Ligand activation of the prokaryotic pentameric ligand-gated ion channel ELIC. PLoS Biol. 2011;9:e1001101 pubmed publisher
    ..The high single channel conductance and the comparably simple functional behavior make ELIC an attractive model system to study general mechanisms of ion conduction and gating in this important family of neurotransmitter receptors...
  8. Sandy M, Butler A. Chrysobactin siderophores produced by Dickeya chrysanthemi EC16. J Nat Prod. 2011;74:1207-12 pubmed publisher
  9. Andrews S, Shipley D, Keen J, Findlay J, Harrison P, Guest J. The haemoglobin-like protein (HMP) of Escherichia coli has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin NADP+ reductases. FEBS Lett. 1992;302:247-52 pubmed
    ..Structure-function properties of other FNR-like proteins, including LuxG and VanB, are also discussed. ..
  10. Peckham G, Kaneshiro W, Luu V, Berestecky J, Alvarez A. Specificity of monoclonal antibodies to strains of Dickeya sp. that cause bacterial heart rot of pineapple. Hybridoma (Larchmt). 2010;29:383-9 pubmed publisher
    ..Use of the two MAbs in field assays also permits the monitoring of a known subpopulation and provides additional decision tools for disease containment and management practices. ..
  11. Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan O, et al. Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine. Nat Commun. 2012;3:714 pubmed publisher
  12. Figueiredo L, Cole P, Drachtman R. Asparaginase Erwinia chrysanthemi as a component of a multi-agent chemotherapeutic regimen for the treatment of patients with acute lymphoblastic leukemia who have developed hypersensitivity to E. coli-derived asparaginase. Expert Rev Hematol. 2016;9:227-34 pubmed publisher
    ..While PEG asparaginase is increasingly used in front-line therapy for ALL, hypersensitivity still occurs with this preparation, and a change to a non-cross-reactive preparation may be necessary. ..
  13. Berensmeier S, Singh S, Meens J, Buchholz K. Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase. Appl Microbiol Biotechnol. 2004;64:560-7 pubmed publisher
    ..The yields of unsaturated trigalacturonic acids were further examined for the substrates polygalacturonic acid, citrus pectin and sugar-beet pectin...
  14. Jahn C, Willis D, Charkowski A. The flagellar sigma factor fliA is required for Dickeya dadantii virulence. Mol Plant Microbe Interact. 2008;21:1431-42 pubmed publisher
    ..dadantii) and two pectate lyase gene homologs, pelX and ABF-0019391. Because FliA is a key contributor to virulence in D. dadantii, it is a new target for disease control. ..
  15. Franza T, Sauvage C, Expert D. Iron regulation and pathogenicity in Erwinia chrysanthemi 3937: role of the Fur repressor protein. Mol Plant Microbe Interact. 1999;12:119-28 pubmed
    ..These findings indicate that, in E. chrysanthemi 3937, (i) Fur negatively controls iron transport and genes encoding PelD and PelE, and (ii) additional factor(s) mediate iron regulation of the pel genes. ..
  16. Thomas X, Cannas G, Chelghoum Y, Gougounon A. [Therapeutic alternatives to native L-asparaginase in the treatment of adult acute lymphoblastic leukemia]. Bull Cancer. 2010;97:1105-17 pubmed publisher
    ..Advantages of these therapeutic alternatives to native L-asparaginase and their results as part of preliminary clinical trials in adults have been outlined in this review. ..
  17. Nasser W, Reverchon S, Condemine G, Robert Baudouy J. Specific interactions of Erwinia chrysanthemi KdgR repressor with different operators of genes involved in pectinolysis. J Mol Biol. 1994;236:427-40 pubmed
    ..These results suggest the existence of different regulation mechanisms mediated by the KdgR protein for the two classes of operators. ..
  18. Johansson K, El Ahmad M, Friemann R, Jornvall H, Markovic O, Eklund H. Crystal structure of plant pectin methylesterase. FEBS Lett. 2002;514:243-9 pubmed
    ..The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state. ..
  19. Costechareyre D, Balmand S, Condemine G, Rahbe Y. Dickeya dadantii, a plant pathogenic bacterium producing Cyt-like entomotoxins, causes septicemia in the pea aphid Acyrthosiphon pisum. PLoS ONE. 2012;7:e30702 pubmed publisher
    ..Finally, the presence of bacteria on the surface of leaves hosting infected aphids indicated that the insects could be vectors of the bacteria. ..
  20. Condemine G, Ghazi A. Differential regulation of two oligogalacturonate outer membrane channels, KdgN and KdgM, of Dickeya dadantii (Erwinia chrysanthemi). J Bacteriol. 2007;189:5955-62 pubmed
    ..KdgN may allow the bacteria to collect oligogalacturonates under saprophytic conditions, when virulence genes, including kdgM, are not expressed. ..
  21. Castang S, Reverchon S, Gouet P, Nasser W. Direct evidence for the modulation of the activity of the Erwinia chrysanthemi quorum-sensing regulator ExpR by acylhomoserine lactone pheromone. J Biol Chem. 2006;281:29972-87 pubmed
  22. Maggiorani Valecillos A, Rodriguez Palenzuela P, Lopez Solanilla E. The role of several multidrug resistance systems in Erwinia chrysanthemi pathogenesis. Mol Plant Microbe Interact. 2006;19:607-13 pubmed
    ..These results indicate that the function of these systems in plants could be related to the specificity to extrude a toxic compound that is present in a given host. ..
  23. Brader G, Sjöblom S, Hyytiäinen H, Sims Huopaniemi K, Palva E. Altering substrate chain length specificity of an acylhomoserine lactone synthase in bacterial communication. J Biol Chem. 2005;280:10403-9 pubmed
    ..Our data identify critical residues that define the size of the substrate-binding pocket of the AHSL synthase and will help in understanding and manipulating this bacterial language...
  24. Edwards M, Henriksen E, Yomano L, Gardner B, Sharma L, Ingram L, et al. Addition of genes for cellobiase and pectinolytic activity in Escherichia coli for fuel ethanol production from pectin-rich lignocellulosic biomass. Appl Environ Microbiol. 2011;77:5184-91 pubmed publisher
    ..The addition of commercial pectin methylesterase (PME) further increased JP08C ethanol production compared to that of LY40A by demethylating the pectin for enzymatic attack by pectin-degrading enzymes. ..
  25. Douet V, Loiseau L, Barras F, Py B. Systematic analysis, by the yeast two-hybrid, of protein interaction between components of the type II secretory machinery of Erwinia chrysanthemi. Res Microbiol. 2004;155:71-5 pubmed
    ..Taken together, these results suggest that OutJ is involved in multiple partnerships. Implications of these partnerships in the overall architecture of the type II secretion machinery are discussed. ..
  26. Hassan S, Hugouvieux Cotte Pattat N. Identification of two feruloyl esterases in Dickeya dadantii 3937 and induction of the major feruloyl esterase and of pectate lyases by ferulic acid. J Bacteriol. 2011;193:963-70 pubmed publisher
    ..Together, these effects of feruloyl esterases could facilitate soft rot disease caused by pectinolytic bacteria. ..
  27. Boughammoura A, Matzanke B, Böttger L, Reverchon S, Lesuisse E, Expert D, et al. Differential role of ferritins in iron metabolism and virulence of the plant-pathogenic bacterium Erwinia chrysanthemi 3937. J Bacteriol. 2008;190:1518-30 pubmed publisher
    ..chrysanthemi depending on the host, indicating the importance of a perfect control of iron homeostasis in this bacterial species during infection. ..
  28. Yang B, Brand J, Gray J, Montgomery R. Extracellular polysaccharides of modified strains of Erwinia spp. Carbohydr Res. 2001;333:295-302 pubmed
    ..chrysanthemi Ech9. These modified E. chrysanthemi spp. have been ribotyped and found to be closely related to their parent strains. ..
  29. Couillault C, Ewbank J. Diverse bacteria are pathogens of Caenorhabditis elegans. Infect Immun. 2002;70:4705-7 pubmed
    ..We show here that the nematode Caenorhabditis elegans is susceptible to a surprisingly broad range of bacteria and may constitute a useful model for the study of both pathogens and symbionts. ..
  30. Jenkins J, Shevchik V, Hugouvieux Cotte Pattat N, Pickersgill R. The crystal structure of pectate lyase Pel9A from Erwinia chrysanthemi. J Biol Chem. 2004;279:9139-45 pubmed publisher
    ..The active site geometries and mechanism of Pel1A and Pel9A are closely similar, but the catalytic base is a lysine in the Pel9A enzymes as opposed to an arginine in the Pel1A enzymes...
  31. Lubkowski J, Dauter M, Aghaiypour K, Wlodawer A, Dauter Z. Atomic resolution structure of Erwinia chrysanthemi L-asparaginase. Acta Crystallogr D Biol Crystallogr. 2003;59:84-92 pubmed
    ..This atomic resolution structure is a step in that direction. ..
  32. Nachin L, El Hassouni M, Loiseau L, Expert D, Barras F. SoxR-dependent response to oxidative stress and virulence of Erwinia chrysanthemi: the key role of SufC, an orphan ABC ATPase. Mol Microbiol. 2001;39:960-72 pubmed
    ..Overall, these results stress the importance of the connection between iron metabolism and oxidative stress during the early steps of infection by E. chrysanthemi...
  33. Sepulchre J, Reverchon S, Nasser W. Modeling the onset of virulence in a pectinolytic bacterium. J Theor Biol. 2007;244:239-57 pubmed
    ..This first modeling of the regulation of pel genes opens the way to new confrontations between theoretical ideas with experiments and possible strategies to fight the soft-rot disease of plants. ..
  34. Shevchik V, Hugouvieux Cotte Pattat N. PaeX, a second pectin acetylesterase of Erwinia chrysanthemi 3937. J Bacteriol. 2003;185:3091-100 pubmed
    ..PaeX is mostly found in the periplasmic space of E. chrysanthemi. These data suggest that PaeX is mainly involved in the deacetylation of esterified oligogalacturonides that enter the periplasm by the KdgM porin. ..
  35. Bierau H, Hinton R, Lyddiatt A. Direct process integration of cell disruption and fluidised bed adsorption in the recovery of labile microbial enzymes. Bioseparation. 2001;10:73-85 pubmed
  36. Login F, Fries M, Wang X, Pickersgill R, Shevchik V. A 20-residue peptide of the inner membrane protein OutC mediates interaction with two distinct sites of the outer membrane secretin OutD and is essential for the functional type II secretion system in Erwinia chrysanthemi. Mol Microbiol. 2010;76:944-55 pubmed publisher
    ..We suggest that successive interactions between these distinct regions of OutC and OutD may have functional importance in switching the secretion machine. ..
  37. Venkatesh B, Babujee L, Liu H, Hedley P, Fujikawa T, Birch P, et al. The Erwinia chrysanthemi 3937 PhoQ sensor kinase regulates several virulence determinants. J Bacteriol. 2006;188:3088-98 pubmed
    ..Our results indicate that the PhoPQ system governs the expression of several additional virulence factors and may also be involved in interactions with other regulatory systems. ..
  38. Kelo E, Noronkoski T, Stoineva I, Petkov D, Mononen I. Beta-aspartylpeptides as substrates of L-asparaginases from Escherichia coli and Erwinia chrysanthemi. FEBS Lett. 2002;528:130-2 pubmed
    ..Our data show that L-asparaginases can hydrolyze beta-aspartylpeptides and suggest that L-asparaginase therapy may affect the metabolism of beta-aspartylpeptides present in human body. ..
  39. Hurlbert J, Preston J. Functional characterization of a novel xylanase from a corn strain of Erwinia chrysanthemi. J Bacteriol. 2001;183:2093-100 pubmed
  40. Zalewska Szewczyk B, Gach A, Wyka K, Bodalski J, Młynarski W. The cross-reactivity of anti-asparaginase antibodies against different L-asparaginase preparations. Clin Exp Med. 2009;9:113-6 pubmed publisher
    ..This finding is of clinical importance with regard to appropriate dosage and necessitates careful enzyme activity monitoring in all patients undergoing repeated treatment with various L-asparaginase preparations. ..
  41. Nasser W, Reverchon S, Vedel R, Boccara M. PecS and PecT coregulate the synthesis of HrpN and pectate lyases, two virulence determinants in Erwinia chrysanthemi 3937. Mol Plant Microbe Interact. 2005;18:1205-14 pubmed
    ..The results suggest coordinated regulation of HrpN and pectate lyases by PecS and PecT. A putative model of the synthesis of these two virulence factors in E. chrysanthemi during pathogenesis is presented. ..
  42. Boccara M, Mills C, Zeier J, Anzi C, Lamb C, Poole R, et al. Flavohaemoglobin HmpX from Erwinia chrysanthemi confers nitrosative stress tolerance and affects the plant hypersensitive reaction by intercepting nitric oxide produced by the host. Plant J. 2005;43:226-37 pubmed
  43. Rincon Enriquez G, Crété P, Barras F, Py B. Biogenesis of Fe/S proteins and pathogenicity: IscR plays a key role in allowing Erwinia chrysanthemi to adapt to hostile conditions. Mol Microbiol. 2008;67:1257-73 pubmed publisher
    ..In this view, the IscR regulator, which controls both Isc and Suf, is predicted to play a major role in the ability of E. chrysanthemi to colonize a wide array of different plants...
  44. Yang C, Gavilanes Ruiz M, Okinaka Y, Vedel R, Berthuy I, Boccara M, et al. hrp genes of Erwinia chrysanthemi 3937 are important virulence factors. Mol Plant Microbe Interact. 2002;15:472-80 pubmed
    ..chrysanthemi and their ability to elicit HR on nonhosts. The data also suggest that other effector proteins secreted by the Hrp system are required for full virulence and HR elicitation...
  45. Hege T, Baumann U. Protease C of Erwinia chrysanthemi: the crystal structure and role of amino acids Y228 and E189. J Mol Biol. 2001;314:187-93 pubmed
    ..The study presented here provides clear evidence that Glu189 of the HEXXHXXGXXH motif is the catalytic base, while Tyr228 is more likely involved in substrate binding and the stabilisation of the tetrahedral transition state...
  46. Willems L, Jacque N, Jacquel A, Neveux N, Maciel T, Lambert M, et al. Inhibiting glutamine uptake represents an attractive new strategy for treating acute myeloid leukemia. Blood. 2013;122:3521-32 pubmed publisher
    ..Finally, we observe a strong autophagic process upon l-ase treatment. These results suggest that l-ase anticancer activity and glutamine uptake inhibition are promising new therapeutic strategies for AML. ..
  47. Kazmierczak B, Mielke D, Russel M, Model P. pIV, a filamentous phage protein that mediates phage export across the bacterial cell envelope, forms a multimer. J Mol Biol. 1994;238:187-98 pubmed
    ..This suggests that the homologous proteins themselves form homo-multimers. These structures could form gated channels that conduct assembling phage or specific substrate proteins across the outer membrane to the extracellular milieu...
  48. Chatterjee A, Liu Y, Chatterjee A. Nucleotide sequence of a pectate lyase structural gene, pel1 of Erwinia carotovora subsp. carotovora strain 71 and structural relationship of pel1 with other pel genes of Erwinia species. Mol Plant Microbe Interact. 1995;8:92-5 pubmed
    ..chrysanthemi pectinases, flank the promoter of pel1. Although pel1 belongs to the pelBC family, it is more closely related to the pel genes of E. carotovora than to the pelBC genes of E. chrysanthemi...
  49. Liu Q, Zhang J, Wang Y, Wang Z. [Genetic and molecular characters of toxin producing Erwinia chrysanthemi pv. zeae]. Wei Sheng Wu Xue Bao. 2008;48:446-51 pubmed
    ..Bacterial foot rot, caused by Erwinia chrysanthemi pv. zeae, is one of the most important diseases in rice. Genetic and molecular characters of toxin producting for Erwinia chrysanthemi pv. zeae were conducted in this paper...
  50. Aruscavage D, Miller S, Ivey M, Lee K, Lejeune J. Survival and dissemination of Escherichia coli O157:H7 on physically and biologically damaged lettuce plants. J Food Prot. 2008;71:2384-8 pubmed
    ..Preharvest damage, especially that caused by traumatic injury, impacted the survivability of E. coli O157:H7. Maintaining healthy plants and minimizing physical damage around the time of harvest might improve the safety of fresh produce...
  51. van der Sluis I, Vrooman L, Pieters R, Baruchel A, Escherich G, Goulden N, et al. Consensus expert recommendations for identification and management of asparaginase hypersensitivity and silent inactivation. Haematologica. 2016;101:279-85 pubmed publisher
    ..We provide a consensus of expert opinions on the role of serum asparaginase level assessment, indications for switching asparaginase preparation, and monitoring after change in asparaginase preparation. ..
  52. Fu S, Tsai T, Chen Y, Liu C, Haiso L, Syue L, et al. Characterization of the early response of the orchid, Phalaenopsis amabilis, to Erwinia chrysanthemi infection using expression profiling. Physiol Plant. 2012;145:406-25 pubmed publisher
    ..In summary, the pathogen-responsive gene expression profiles facilitated a more comprehensive view of the molecular events that underlie this economically important plant-pathogen interaction...
  53. Cogez V, Talaga P, Lemoine J, Bohin J. Osmoregulated periplasmic glucans of Erwinia chrysanthemi. J Bacteriol. 2001;183:3127-33 pubmed
    ..This glucan backbone may be substituted by O-acetyl and O-succinyl ester-linked residues...
  54. Llama Palacios A, Lopez Solanilla E, Poza Carrión C, García Olmedo F, Rodriguez Palenzuela P. The Erwinia chrysanthemi phoP-phoQ operon plays an important role in growth at low pH, virulence and bacterial survival in plant tissue. Mol Microbiol. 2003;49:347-57 pubmed
    ..This gene was induced at low Mg(2+) concentrations and in planta. These results suggest that E. chrysanthemi PhoP-PhoQ system plays an important role in bacterial survival in plant tissues during the initial infection stages...
  55. Bouley J, Condemine G, Shevchik V. The PDZ domain of OutC and the N-terminal region of OutD determine the secretion specificity of the type II out pathway of Erwinia chrysanthemi. J Mol Biol. 2001;308:205-19 pubmed
    ..This strongly indicates that diverse exoproteins possess a variable number of targeting signals which are recognised by different regions of OutC and OutD...
  56. St John F, Hurlbert J, Rice J, Preston J, Pozharski E. Ligand bound structures of a glycosyl hydrolase family 30 glucuronoxylan xylanohydrolase. J Mol Biol. 2011;407:92-109 pubmed publisher
    ..Our results identify the molecular interactions that define the unique function of XynC and homologous GH30 enzymes...
  57. Loiseau L, Ollagnier de Choudens S, Nachin L, Fontecave M, Barras F. Biogenesis of Fe-S cluster by the bacterial Suf system: SufS and SufE form a new type of cysteine desulfurase. J Biol Chem. 2003;278:38352-9 pubmed publisher
    ..This is the first example of a two-component cysteine desulfurase enzyme...
  58. Expert D, Boughammoura A, Franza T. Siderophore-controlled iron assimilation in the enterobacterium Erwinia chrysanthemi: evidence for the involvement of bacterioferritin and the Suf iron-sulfur cluster assembly machinery. J Biol Chem. 2008;283:36564-72 pubmed publisher
    ..Our data demonstrate a functional link between bacterioferritin, bacterioferredoxin, and the Suf protein machinery resulting in optimal bacterial growth and a balanced distribution of iron between essential metalloproteins...
  59. Schrey D, Borghorst S, Lanvers Kaminsky C, Hempel G, Gerss J, Möricke A, et al. Therapeutic drug monitoring of asparaginase in the ALL-BFM 2000 protocol between 2000 and 2007. Pediatr Blood Cancer. 2010;54:952-8 pubmed publisher
  60. Dellagi A, Rigault M, Segond D, Roux C, Kraepiel Y, Cellier F, et al. Siderophore-mediated upregulation of Arabidopsis ferritin expression in response to Erwinia chrysanthemi infection. Plant J. 2005;43:262-72 pubmed
    ..chrysanthemi is a basal defense mechanism which is mainly activated by bacterial siderophores. The potential role of siderophores in this process is discussed...
  61. Louw C, La Grange D, Pretorius I, Van Rensburg P. The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavour. J Biotechnol. 2006;125:447-61 pubmed
    ..The Pinot Noir wines were also sensorial evaluated and the tasting panel preferred the wines fermented with the recombinant strains...
  62. Rojas C, Ham J, Schechter L, Kim J, Beer S, Collmer A. The Erwinia chrysanthemi EC16 hrp/hrc gene cluster encodes an active Hrp type III secretion system that is flanked by virulence genes functionally unrelated to the Hrp system. Mol Plant Microbe Interact. 2004;17:644-53 pubmed
    ..chrysanthemi Hrp regulator genes hrpL and hrpS. Thus, the 44-kb region of the E. chrysanthemi EC16 genome that is centered on the hrplhrc cluster encodes a potpourri of virulence factors, but none of these appear to be a TTSS effector...
  63. McMahon S, Oke M, Liu H, Johnson K, Carter L, Kadi N, et al. Purification, crystallization and data collection of Pectobacterium chrysanthemi AcsD, a type A siderophore synthetase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008;64:1052-5 pubmed publisher
    AcsD, a type A siderophore synthetase with a molecular weight of 71 140 Da from Pectobacterium chrysanthemi, has been expressed, purified and crystallized at 293 K...
  64. Potrykus M, Golanowska M, Hugouvieux Cotte Pattat N, Lojkowska E. Regulators involved in Dickeya solani virulence, genetic conservation, and functional variability. Mol Plant Microbe Interact. 2014;27:700-11 pubmed publisher
    ..The thermoregulator PecT seems to be a major regulator of D. solani virulence. This work also reveals the role of quorum sensing mediated by ExpI and ExpR in D. solani virulence on potato...
  65. Rouanet C, Nasser W. The PecM protein of the phytopathogenic bacterium Erwinia chrysanthemi, membrane topology and possible involvement in the efflux of the blue pigment indigoidine. J Mol Microbiol Biotechnol. 2001;3:309-18 pubmed
    ..chrysanthemi derivative isogenic mutants pecS, pecM and pecS-pecM revealed that PecM is required for complete efflux of the pigment. Its relation to other efflux systems and its potential physiological role are discussed...
  66. Laurent F, Kotoujansky A, Bertheau Y. Overproduction in Escherichia coli of the pectin methylesterase A from Erwinia chrysanthemi 3937: one-step purification, biochemical characterization, and production of polyclonal antibodies. Can J Microbiol. 2000;46:474-80 pubmed
    ..It recognized PMEs from Erwinia species, but did not cross-react with PME of fungal or plant origin, and will therefore be a useful tool to immunolocalize the protein during plant-pathogen interactions...
  67. Yang B, Brand J, Montgomery R. Pyruvated galactose and oligosaccharides from Erwinia chrysanthemi Ech6 extracellular polysaccharide. Carbohydr Res. 2001;331:59-67 pubmed
    ..Y.; Gray, J. S. S.; Montgomery, R. Int. J. Biol. Macromol., 1994, 16, 306-312]...
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    ..Marker exchange mutagenesis of the xynA gene eliminated the ability of strain D1 to produce detectable extracellular xylanase activity but did not affect virulence on corn leaves...
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    ..Moreover, indigoidine production conferred an increased resistance to oxidative stress, indicating that indigoidine may protect the bacteria against the reactive oxygen species generated during the plant defense response...
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    ..chrysanthemi supernatants. In addition to the already known substrates (eleven pectinases and one cellulase), this analysis revealed that the type II Out system mediates secretion of the esterase FaeD and of the Avr-like protein AvrL...
  72. Brandl M. Plant lesions promote the rapid multiplication of Escherichia coli O157:H7 on postharvest lettuce. Appl Environ Microbiol. 2008;74:5285-9 pubmed publisher
    ..coli O157:H7 over a short time and suggest that harvesting and processing are critical control points in the prevention or reduction of E. coli O157:H7 contamination of lettuce...
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    ..The kduD gene was located at 126.8 min of the Erwinia carotovora subsp. atroseptica genetic map...
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    ..Based on the indications of different protocols, lack of cross-reactivity to the E. coli-derived products and taking into consideration regulatory factors and availability, crisantaspase may be considered a viable second-line therapy...
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    ..An eps mutant was less efficient than the wild-type strain in initiating a maceration symptom, suggesting that production of EPS is required for the full expression of the E. chrysanthemi virulence...
  78. Hugouvieux Cotte Pattat N, Blot N, Reverchon S. Identification of TogMNAB, an ABC transporter which mediates the uptake of pectic oligomers in Erwinia chrysanthemi 3937. Mol Microbiol. 2001;41:1113-23 pubmed
    ..The decreased capacity of maceration of a togM mutant indicates the importance of transport and/or attraction of oligogalacturonides for E. chrysanthemi pathogenicity...
  79. Avrova A, Hyman L, Toth R, Toth I. Application of amplified fragment length polymorphism fingerprinting for taxonomy and identification of the soft rot bacteria Erwinia carotovora and Erwinia chrysanthemi. Appl Environ Microbiol. 2002;68:1499-508 pubmed
    ..AFLP fingerprinting was also found to be more differentiating than other techniques for typing the soft rot erwinias and was applicable to all strain types, including different serogroups...
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    ..Differences and similarities in polypeptide trace, size and volume of the active-site groove and surface electrostatics are discussed...
  81. Haque M, Kabir M, Aini L, Hirata H, Tsuyumu S. SlyA, a MarR family transcriptional regulator, is essential for virulence in Dickeya dadantii 3937. J Bacteriol. 2009;191:5409-18 pubmed publisher
    ..These results suggest that SlyA plays an important role in virulence to plants by positively regulating the expression of multiple pathogenicity-related traits of D. dadantii 3937...
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    ..Among these, mutations of Bacillus subtilis pps1, Escherichia coli purU, and Pseudomonas aeruginosa pheC homologues reduced virulence on African violet leaves. Thus, new insights were obtained into genes important in bacterial virulence...
  83. Goude R, Renaud S, Bonnassie S, Bernard T, Blanco C. Glutamine, glutamate, and alpha-glucosylglycerate are the major osmotic solutes accumulated by Erwinia chrysanthemi strain 3937. Appl Environ Microbiol. 2004;70:6535-41 pubmed
  84. Rauscher L, Expert D, Matzanke B, Trautwein A. Chrysobactin-dependent iron acquisition in Erwinia chrysanthemi. Functional study of a homolog of the Escherichia coli ferric enterobactin esterase. J Biol Chem. 2002;277:2385-95 pubmed
    ..The CbsH protein may therefore be regarded as a peptidase that prevents the bacterial cells from being intracellularly iron-depleted by chrysobactin...
  85. Llama Palacios A, Lopez Solanilla E, Rodriguez Palenzuela P. The ybiT gene of Erwinia chrysanthemi codes for a putative ABC transporter and is involved in competitiveness against endophytic bacteria during infection. Appl Environ Microbiol. 2002;68:1624-30 pubmed
    ..These results indicate that the ybiT gene plays a role in the in planta fitness of the bacteria...
  86. Filgueira de Azevedo W, Canduri F, Simões de Oliveira J, Basso L, Palma M, Pereira J, et al. Molecular model of shikimate kinase from Mycobacterium tuberculosis. Biochem Biophys Res Commun. 2002;295:142-8 pubmed
    ..tuberculosis shikimate kinase that should provide a structural framework on which the design of specific inhibitors may be based...
  87. Yap M, Yang C, Barak J, Jahn C, Charkowski A. The Erwinia chrysanthemi type III secretion system is required for multicellular behavior. J Bacteriol. 2005;187:639-48 pubmed
    ..Since the TTSS, but not the cellulose synthase subunit, is required for E. chrysanthemi pellicle formation, this inexpensive assay can be used as a high throughput screen for TTSS mutants or inhibitors...
  88. Ham J, Majerczak D, Arroyo Rodriguez A, Mackey D, Coplin D. WtsE, an AvrE-family effector protein from Pantoea stewartii subsp. stewartii, causes disease-associated cell death in corn and requires a chaperone protein for stability. Mol Plant Microbe Interact. 2006;19:1092-102 pubmed
    ..However, WtsF was required for full accumulation of WtsE within the bacteria at low temperatures. In contrast, WtsF was needed for efficient delivery of WtsE from E. coli via the Erwinia chrysanthemi Hrp system...