escherichia coli k12

Summary

Summary: A species of gram-negative, rod-shaped bacteria belonging to the K serogroup of ESCHERICHIA COLI. It lives as a harmless inhabitant of the human LARGE INTESTINE and is widely used in medical and GENETIC RESEARCH.

Top Publications

  1. Park S, Lee S, Lee J, Nishi K, Kim Y, Jung C, et al. High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement. J Mol Biol. 2008;376:1426-37 pubmed publisher
  2. Gong X, Fan S, Bilderbeck A, Li M, Pang H, Tao S. Comparative analysis of essential genes and nonessential genes in Escherichia coli K12. Mol Genet Genomics. 2008;279:87-94 pubmed
    ..conservation, protein length distribution and amino acid usage between essential and nonessential genes in Escherichia coli K12 demonstrated that essential genes are relatively preserved throughout the bacterial kingdom when compared ..
  3. Walsh C, Duffy G, Nally P, O Mahony R, McDowell D, Fanning S. Transfer of ampicillin resistance from Salmonella Typhimurium DT104 to Escherichia coli K12 in food. Lett Appl Microbiol. 2008;46:210-5 pubmed
    ..the transfer of antibiotic resistance from a donor Salmonella Typhimurium DT104 strain to a recipient Escherichia coli K12 strain...
  4. McCusker M, Turner E, Dorman C. DNA sequence heterogeneity in Fim tyrosine-integrase recombinase-binding elements and functional motif asymmetries determine the directionality of the fim genetic switch in Escherichia coli K-12. Mol Microbiol. 2008;67:171-87 pubmed
  5. Centore R, Lestini R, Sandler S. XthA (Exonuclease III) regulates loading of RecA onto DNA substrates in log phase Escherichia coli cells. Mol Microbiol. 2008;67:88-101 pubmed
    ..Furthermore, ExoI, ExoIII and the other 3'-5' exonucleases process these DSBs, antagonizing the RecBCD pathway of RecA loading, thus regulating the availability of these substrates for recombination...
  6. Subedi K, Kim I, Kim J, Min B, Park C. Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12. FEMS Microbiol Lett. 2008;279:180-7 pubmed publisher
  7. Pinske C, Sawers R. A-type carrier protein ErpA is essential for formation of an active formate-nitrate respiratory pathway in Escherichia coli K-12. J Bacteriol. 2012;194:346-53 pubmed publisher
    ..These findings suggest that ErpA and IscA have overlapping roles in assembly of these anaerobic respiratory enzymes but demonstrate that ErpA is essential for the production of active enzymes...
  8. Castañeda Ramírez J, de la Fuente Salcido N, Salcedo Hernández R, León Galván F, Bideshi D, Barboza Corona J. High-level synthesis of endochitinase ChiA74 in Escherichia coli K12 and its promising potential for use in biotechnology. Folia Microbiol (Praha). 2013;58:455-62 pubmed publisher
    In the present study, we expressed the chiA74 gene of Bacillus thuringiensis in Escherichia coli K12 and demonstrated that the active ChiA74 enzyme was produced at a high level in this strain...
  9. Prieto A, Kahramanoglou C, Ali R, Fraser G, Seshasayee A, Luscombe N. Genomic analysis of DNA binding and gene regulation by homologous nucleoid-associated proteins IHF and HU in Escherichia coli K12. Nucleic Acids Res. 2012;40:3524-37 pubmed publisher
    ..Here we present a genome-scale study of HU- and IHF binding to the Escherichia coli K12 chromosome using ChIP-seq...

More Information

Publications67

  1. Fabich A, Leatham M, Grissom J, Wiley G, Lai H, Najar F, et al. Genotype and phenotypes of an intestine-adapted Escherichia coli K-12 mutant selected by animal passage for superior colonization. Infect Immun. 2011;79:2430-9 pubmed publisher
    ..Hence, mutations that enhance metabolic efficiency confer a colonization advantage...
  2. Portnoy V, Scott D, Lewis N, Tarasova Y, Osterman A, Palsson B. Deletion of genes encoding cytochrome oxidases and quinol monooxygenase blocks the aerobic-anaerobic shift in Escherichia coli K-12 MG1655. Appl Environ Microbiol. 2010;76:6529-40 pubmed publisher
    ..Flux and transcriptomics data were highly correlated and showed similar patterns. ..
  3. Rolfe M, Ter Beek A, Graham A, Trotter E, Asif H, Sanguinetti G, et al. Transcript profiling and inference of Escherichia coli K-12 ArcA activity across the range of physiologically relevant oxygen concentrations. J Biol Chem. 2011;286:10147-54 pubmed publisher
  4. Ahmetagic A, Pemberton J. Antibiotic resistant mutants of Escherichia coli K12 show increases in heterologous gene expression. Plasmid. 2011;65:51-7 pubmed publisher
    ..of antibiotics, it was found that nine separate isolates of spontaneous antibiotic resistant mutants of Escherichia coli K12 pPSX-vioABCDE overproduce the anti-tumour antibiotic violacein...
  5. Li G, Liu B, Ma Q, Xu Y. A new framework for identifying cis-regulatory motifs in prokaryotes. Nucleic Acids Res. 2011;39:e42 pubmed publisher
    ..The power of BOBRO in dealing with noisy data was further demonstrated through identification of the motifs of the global transcriptional regulators by running it over 2390 promoter sequences of Escherichia coli K12.
  6. Westra E, Swarts D, Staals R, Jore M, Brouns S, van der Oost J. The CRISPRs, they are a-changin': how prokaryotes generate adaptive immunity. Annu Rev Genet. 2012;46:311-39 pubmed publisher
    ..Because several excellent recent reviews cover all CRISPR subtypes, we mainly focus on a detailed description of the type I-E CRISPR/Cas system of the model bacterium Escherichia coli K12.
  7. Applebee M, Joyce A, Conrad T, Pettigrew D, Palsson B. Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli. J Biol Chem. 2011;286:23150-9 pubmed publisher
    ..This study demonstrates that fitness correlations can be used to interrogate adaptive processes at the protein level and to identify the regulatory constraints underlying selection and improved growth...
  8. Orth J, Palsson B. Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions. BMC Syst Biol. 2012;6:30 pubmed publisher
    ..There are a total of 208 blocked metabolites in iJO1366, representing gaps in the network...
  9. Freddolino P, Amini S, Tavazoie S. Newly identified genetic variations in common Escherichia coli MG1655 stock cultures. J Bacteriol. 2012;194:303-6 pubmed publisher
    ..The mutations are likely to cause loss of function of the glpR and crl genes, which may have serious implications for physiological experiments using the affected strains...
  10. Ling H, Kang A, Tan M, Qi X, Chang M. The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12. Biochem Biophys Res Commun. 2010;401:521-6 pubmed publisher
    ..Herein, we show that luxS deletion significantly increases swimming motility and flagella synthesis in Escherichia coli K12 using motility, transcriptome, and scanning electron microscopy assays...
  11. Keseler I, Collado Vides J, Santos Zavaleta A, Peralta Gil M, Gama Castro S, Muñiz Rascado L, et al. EcoCyc: a comprehensive database of Escherichia coli biology. Nucleic Acids Res. 2011;39:D583-90 pubmed publisher
    ..The graphical representation of signal transduction pathways has been updated, and the cellular and regulatory overviews were enhanced with new functionality. A specialized undergraduate teaching resource using EcoCyc is being developed...
  12. Cho B, Federowicz S, Embree M, Park Y, Kim D, Palsson B. The PurR regulon in Escherichia coli K-12 MG1655. Nucleic Acids Res. 2011;39:6456-64 pubmed publisher
    ..Compared to the functional diversity of the regulon of the other general transcription factors in E. coli, the functions and size of the PurR regulon are limited...
  13. Ahmetagic A, Philip D, Sarovich D, Kluver D, Pemberton J. Plasmid encoded antibiotics inhibit protozoan predation of Escherichia coli K12. Plasmid. 2011;66:152-8 pubmed publisher
    ..In the current experiments a range of Escherichia coli K12 strains were genetically engineered to produce violacein and a number of its coloured, biosynthetic ..
  14. Wiedenheft B, Lander G, Zhou K, Jore M, Brouns S, van der Oost J, et al. Structures of the RNA-guided surveillance complex from a bacterial immune system. Nature. 2011;477:486-489 pubmed publisher
    ..This conformational rearrangement may serve as a signal that recruits a trans-acting nuclease (Cas3) for destruction of invading nucleic-acid sequences...
  15. Gama Castro S, Salgado H, Peralta Gil M, Santos Zavaleta A, Muñiz Rascado L, Solano Lira H, et al. RegulonDB version 7.0: transcriptional regulation of Escherichia coli K-12 integrated within genetic sensory response units (Gensor Units). Nucleic Acids Res. 2011;39:D98-105 pubmed publisher
    ..A new portable drawing tool for genomic features is also now available, as well as new ways to download the data, including web services, files for several relational database manager systems and text files including BioPAX format...
  16. Waegeman H, Beauprez J, Moens H, Maertens J, De Mey M, Foulquié Moreno M, et al. Effect of iclR and arcA knockouts on biomass formation and metabolic fluxes in Escherichia coli K12 and its implications on understanding the metabolism of Escherichia coli BL21 (DE3). BMC Microbiol. 2011;11:70 pubmed publisher
    ..coli K12 MG1655 under glucose abundant and limiting conditions and compares the results with the metabolic characteristics of E. coli BL21 (DE3)...
  17. Dreyfus M, Heurgué Hamard V. Termination troubles in Escherichia coli K12. Mol Microbiol. 2011;79:288-91 pubmed publisher
    In this issue of Molecular Microbiology, Schaub and Hayes report that, compared with other enterobacteria, Escherichia coli K12 carries two mutations - one in the prfB gene encoding the release factor RF2, and the other in the rpsG gene ..
  18. McVicker G, Sun L, Sohanpal B, Gashi K, Williamson R, Plumbridge J, et al. SlyA protein activates fimB gene expression and type 1 fimbriation in Escherichia coli K-12. J Biol Chem. 2011;286:32026-35 pubmed publisher
    ..This study extends our understanding of the role that SlyA plays in the host-bacterial relationship...
  19. Serra D, Richter A, Klauck G, Mika F, Hengge R. Microanatomy at cellular resolution and spatial order of physiological differentiation in a bacterial biofilm. MBio. 2013;4:e00103-13 pubmed publisher
    ..quot;..
  20. Salgado H, Peralta Gil M, Gama Castro S, Santos Zavaleta A, Muñiz Rascado L, García Sotelo J, et al. RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more. Nucleic Acids Res. 2013;41:D203-13 pubmed publisher
    ..Altogether, RegulonDB version 8.0 is a much better home for integrating knowledge on gene regulation from the sources of information currently available...
  21. Keseler I, Mackie A, Peralta Gil M, Santos Zavaleta A, Gama Castro S, Bonavides Martínez C, et al. EcoCyc: fusing model organism databases with systems biology. Nucleic Acids Res. 2013;41:D605-12 pubmed publisher
    ..coli biology. The utility of EcoCyc is enhanced by new tools available on the EcoCyc web site, and the development of EcoCyc as a teaching tool is increasing the impact of the knowledge collected in EcoCyc...
  22. Singh S, SaiSree L, Amrutha R, Reddy M. Three redundant murein endopeptidases catalyse an essential cleavage step in peptidoglycan synthesis of Escherichia coli K12. Mol Microbiol. 2012;86:1036-51 pubmed publisher
    ..Our results indicate the step of cross-link cleavage as essential for enlargement of the murein sacculus, rendering it a novel target for development of antibacterial therapeutic agents...
  23. Waegeman H, De Lausnay S, Beauprez J, Maertens J, De Mey M, Soetaert W. Increasing recombinant protein production in Escherichia coli K12 through metabolic engineering. N Biotechnol. 2013;30:255-61 pubmed publisher
    ..coli BL21 (DE3), that is, 27±5 mg g(CDW)(-1) vs. 30±5 mg g(CDW)(-1), respectively...
  24. Westra E, van Erp P, Künne T, Wong S, Staals R, Seegers C, et al. CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3. Mol Cell. 2012;46:595-605 pubmed publisher
    ..The target is then progressively unwound and cleaved by the joint ATP-dependent helicase activity and Mg(2+)-dependent HD-nuclease activity of Cas3, leading to complete target DNA degradation and invader neutralization...
  25. Orth J, Conrad T, Na J, Lerman J, Nam H, Feist A, et al. A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011. Mol Syst Biol. 2011;7:535 pubmed publisher
    ..Like its predecessors, the iJO1366 reconstruction is expected to be widely deployed for studying the systems biology of E. coli and for metabolic engineering applications...
  26. Negrete A, Majdalani N, Phue J, Shiloach J. Reducing acetate excretion from E. coli K-12 by over-expressing the small RNA SgrS. N Biotechnol. 2013;30:269-73 pubmed publisher
    ..coli B. This observation opens a new approach towards controlling bacterial metabolism through the use of noncoding RNA...
  27. Westra E, Pul U, Heidrich N, Jore M, Lundgren M, Stratmann T, et al. H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO. Mol Microbiol. 2010;77:1380-93 pubmed publisher
    ..Overexpression of LeuO in E. coli K12 containing an anti-Lambda CRISPR leads to an enhanced protection against phage infection. This study demonstrates that in E. coli H-NS and LeuO are antagonistic regulators of CRISPR-based immunity...
  28. Gunasekera T, Csonka L, Paliy O. Genome-wide transcriptional responses of Escherichia coli K-12 to continuous osmotic and heat stresses. J Bacteriol. 2008;190:3712-20 pubmed publisher
    ..This finding does not support the hypothesis that K(+) is the central osmoregulatory signal in Enterobacteriaceae. ..
  29. Handford J, Ize B, Buchanan G, Butland G, Greenblatt J, Emili A, et al. Conserved network of proteins essential for bacterial viability. J Bacteriol. 2009;191:4732-49 pubmed publisher
    ..study, we have constructed conditional expression strains for each of these genes in the model organism Escherichia coli K12. We show that each gene is essential for the viability of E. coli under laboratory growth conditions...
  30. Gama Castro S, Jimenez Jacinto V, Peralta Gil M, Santos Zavaleta A, Peñaloza Spinola M, Contreras Moreira B, et al. RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation. Nucleic Acids Res. 2008;36:D120-4 pubmed
    ..is the primary reference database offering curated knowledge of the transcriptional regulatory network of Escherichia coli K12, currently the best-known electronically encoded database of the genetic regulatory network of any free-..
  31. Kurakata Y, Uechi A, Yoshida H, Kamitori S, Sakano Y, Nishikawa A, et al. Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63. J Mol Biol. 2008;381:116-28 pubmed publisher
    ..Here we determined the crystal structure of a bacterial GH63 enzyme, Escherichia coli K12 YgjK, at 1.78 A resolution and investigated some properties of the enzyme...
  32. Beloin C, Roux A, Ghigo J. Escherichia coli biofilms. Curr Top Microbiol Immunol. 2008;322:249-89 pubmed
    ..This chapter will present known molecular mechanisms underlying biofilm development in both commensal and pathogenic E. coli...
  33. Tang X, Tan Y, Zhu H, Zhao K, Shen W. Microbial conversion of glycerol to 1,3-propanediol by an engineered strain of Escherichia coli. Appl Environ Microbiol. 2009;75:1628-34 pubmed publisher
    ..To our knowledge, this is the highest reported yield and productivity efficiency of 1,3-PD with glycerol as the sole source of carbon. Furthermore, the overall fermentation time was only 40 h, shorter than that of any other reports...
  34. McLean S, Bowman L, Sanguinetti G, Read R, Poole R. Peroxynitrite toxicity in Escherichia coli K12 elicits expression of oxidative stress responses and protein nitration and nitrosylation. J Biol Chem. 2010;285:20724-31 pubmed publisher
    ..Thus, the cellular responses to peroxynitrite and hydrogen peroxide are distinct...
  35. Taymaz Nikerel H, Borujeni A, Verheijen P, Heijnen J, van Gulik W. Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry. Biotechnol Bioeng. 2010;107:369-81 pubmed publisher
    ..The resulting metabolic network model only requires the specific rate of growth, micro, as an input in order to accurately predict all other fluxes and yields...
  36. Yang K, Wang M, Metcalf W. Uptake of glycerol-2-phosphate via the ugp-encoded transporter in Escherichia coli K-12. J Bacteriol. 2009;191:4667-70 pubmed publisher
    ..Mutations in the ugpBAECQ operon eliminated this phenotype, suggesting that G2P is a previously unrecognized substrate for the binding protein-dependent Ugp transporter...
  37. Dong T, Kirchhof M, Schellhorn H. RpoS regulation of gene expression during exponential growth of Escherichia coli K12. Mol Genet Genomics. 2008;279:267-77 pubmed
    ..In summary, the identification of a group of exponential phase genes controlled by RpoS reveals a novel aspect of RpoS function...
  38. Klein G, Lindner B, Brabetz W, Brade H, Raina S. Escherichia coli K-12 Suppressor-free Mutants Lacking Early Glycosyltransferases and Late Acyltransferases: minimal lipopolysaccharide structure and induction of envelope stress response. J Biol Chem. 2009;284:15369-89 pubmed publisher
    ..This suppression could be alleviated by overexpression of certain mutant msbA alleles or the single-copy chromosomal MsbA-498V variant in the vicinity of Walker-box II...
  39. Collado Vides J, Salgado H, Morett E, Gama Castro S, Jimenez Jacinto V, Martinez Flores I, et al. Bioinformatics resources for the study of gene regulation in bacteria. J Bacteriol. 2009;191:23-31 pubmed publisher
  40. Karp P, Keseler I, Shearer A, Latendresse M, Krummenacker M, Paley S, et al. Multidimensional annotation of the Escherichia coli K-12 genome. Nucleic Acids Res. 2007;35:7577-90 pubmed
    ..coli, such as dead-end metabolites. EcoCyc provides a genome browser with novel properties, and a novel interactive display of transcriptional regulatory networks...
  41. Zhang S, Xu M, Li S, Su Z. Genome-wide de novo prediction of cis-regulatory binding sites in prokaryotes. Nucleic Acids Res. 2009;37:e72 pubmed publisher
    ..can predict 81% of known individual binding sites belonging to 94% of known cis-regulatory motifs in the Escherichia coli K12 genome, while achieving high prediction specificity...
  42. Sommerfeldt N, Possling A, Becker G, Pesavento C, Tschowri N, Hengge R. Gene expression patterns and differential input into curli fimbriae regulation of all GGDEF/EAL domain proteins in Escherichia coli. Microbiology. 2009;155:1318-31 pubmed publisher
    ..Finally, we discuss why other GGDEF/EAL domain-encoding genes, despite being expressed, do not influence motility and/or curli formation...
  43. Leparc G, Tüchler T, Striedner G, Bayer K, Sykacek P, Hofacker I, et al. Model-based probe set optimization for high-performance microarrays. Nucleic Acids Res. 2009;37:e18 pubmed publisher
    ..The algorithm and features are discussed and demonstrated on actual design runs. Source code is available on request...
  44. Seshasayee A, Fraser G, Babu M, Luscombe N. Principles of transcriptional regulation and evolution of the metabolic system in E. coli. Genome Res. 2009;19:79-91 pubmed publisher
    ..genome-scale data to investigate the evolutionary trends and transcriptional control of metabolism in Escherichia coli K12. Globally, the regulatory system is organized in a clear hierarchy of general and specific transcription ..
  45. Durfee T, Hansen A, Zhi H, Blattner F, Jin D. Transcription profiling of the stringent response in Escherichia coli. J Bacteriol. 2008;190:1084-96 pubmed
    ..However, one-half of the up-regulated genes have unknown functions. How these results are correlated with the various effects of (p)ppGpp (in particular, RNA polymerase redistribution) is discussed...
  46. Kim K, Pelton J, Inwood W, Andersen U, Kustu S, Wemmer D. The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems. J Bacteriol. 2010;192:4089-102 pubmed publisher
    ..The function of RutE appears to be the same as that of YdfG, which reduces malonic semialdehyde to 3-hydroxypropionic acid. RutG appears to be a uracil transporter...
  47. Nahku R, Valgepea K, Lahtvee P, Erm S, Abner K, Adamberg K, et al. Specific growth rate dependent transcriptome profiling of Escherichia coli K12 MG1655 in accelerostat cultures. J Biotechnol. 2010;145:60-5 pubmed publisher
    Specific growth rate dependent gene expression changes of Escherichia coli K12 MG1655 were studied by microarray and real-time PCR analyses...
  48. Guymer D, Maillard J, Sargent F. A genetic analysis of in vivo selenate reduction by Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12. Arch Microbiol. 2009;191:519-28 pubmed publisher
    ..Further mutagenesis and in vitro biophysical experiments implicate the Salmonella ynfE gene product, and the E. coli YnfE and YnfF proteins, as putative Tat-targeted selenate reductases...
  49. Rodrigues D, Elimelech M. Role of type 1 fimbriae and mannose in the development of Escherichia coli K12 biofilm: from initial cell adhesion to biofilm formation. Biofouling. 2009;25:401-11 pubmed publisher
    ..structures, on biofilm development and maturation has been examined by the use of three isogenic Escherichia coli K12 strains: wild type, fimbriated, and non-fimbriated...
  50. Pesavento C, Becker G, Sommerfeldt N, Possling A, Tschowri N, Mehlis A, et al. Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli. Genes Dev. 2008;22:2434-46 pubmed publisher
    ..In summary, these data reveal the logic and sequence of molecular events underlying the motile-to-adhesive "lifestyle" switch in E. coli...
  51. Rea D, Hovington R, Rakus J, Gerlt J, Fulop V, Bugg T, et al. Crystal structure and functional assignment of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12. Biochemistry. 2008;47:9955-65 pubmed publisher
    ..Crystal structures suggest these enzymes use an Asp-His intersubunit dyad to activate a metal-bound water or hydroxide for proton transfer during catalysis...
  52. Rodriguez Penagos C, Salgado H, Martinez Flores I, Collado Vides J. Automatic reconstruction of a bacterial regulatory network using Natural Language Processing. BMC Bioinformatics. 2007;8:293 pubmed
    ..We implemented a rule-based system to generate networks from different sets of documents dealing with regulation in Escherichia coli K-12...
  53. Esnault E, Valens M, Espeli O, Boccard F. Chromosome structuring limits genome plasticity in Escherichia coli. PLoS Genet. 2007;3:e226 pubmed
    ..Consequences for the control of the bacterial cell cycle and for the evolution of bacterial chromosome configuration are discussed...
  54. Brouns S, Jore M, Lundgren M, Westra E, Slijkhuis R, Snijders A, et al. Small CRISPR RNAs guide antiviral defense in prokaryotes. Science. 2008;321:960-4 pubmed publisher
    ..Our results demonstrate that the formation of mature guide RNAs by the CRISPR RNA endonuclease subunit of Cascade is a mechanistic requirement for antiviral defense...
  55. Lemuth K, Hardiman T, Winter S, Pfeiffer D, Keller M, Lange S, et al. Global transcription and metabolic flux analysis of Escherichia coli in glucose-limited fed-batch cultivations. Appl Environ Microbiol. 2008;74:7002-15 pubmed publisher
    ..The present work shows that detailed understanding of the genetic regulation of bacterial metabolism provides useful insights for manipulating the carbon flux in technical production processes...
  56. Konopka M, Sochacki K, Bratton B, Shkel I, Record M, Weisshaar J. Cytoplasmic protein mobility in osmotically stressed Escherichia coli. J Bacteriol. 2009;191:231-7 pubmed publisher
    ..Recovery of the ability of proteins to diffuse in the cytoplasm after plasmolysis may well be a key determinant of the time scale of the recovery of growth...
  57. Butala M, Busby S, Lee D. DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes. Nucleic Acids Res. 2009;37:e37 pubmed publisher
    ..We illustrate the method by investigating the proteins bound to the colicin K gene regulatory region, either before or after induction of the colicin K gene promoter...
  58. Yamamoto N, Nakahigashi K, Nakamichi T, Yoshino M, Takai Y, Touda Y, et al. Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol Syst Biol. 2009;5:335 pubmed publisher