escherichia coli


Summary: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

Top Publications

  1. Bisicchia P, Steel B, Mariam Debela M, Löwe J, Sherratt D. The N-terminal membrane-spanning domain of the Escherichia coli DNA translocase FtsK hexamerizes at midcell. MBio. 2013;4:e00800-13 pubmed publisher
    ..Using single-molecule live-cell imaging, we show that newborn Escherichia coli cells growing in minimal medium contain ~40 membrane-bound FtsK molecules that are largely monomeric; the ..
  2. Gardner S, Johns K, Tanner R, McCleary W. The PhoU protein from Escherichia coli interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane. J Bacteriol. 2014;196:1741-52 pubmed publisher
    ..transporter PstSCAB and the two-component proteins PhoR and PhoB, is absolutely required for Pi signaling in Escherichia coli. Little is known about the role of PhoU and its function in regulation...
  3. Low H, Gubellini F, Rivera Calzada A, Braun N, Connery S, Dujeancourt A, et al. Structure of a type IV secretion system. Nature. 2014;508:550-553 pubmed publisher
    ..Here we use electron microscopy to reconstruct the type IV secretion system encoded by the Escherichia coli R388 conjugative plasmid...
  4. Hao Z, Lou H, Zhu R, Zhu J, Zhang D, Zhao B, et al. The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli. Nat Chem Biol. 2014;10:21-8 pubmed publisher
    ..The natural inducer for Escherichia coli MarR, the prototypical transcription repressor within this family, remains unknown...
  5. Secher T, Samba Louaka A, Oswald E, Nougayrede J. Escherichia coli producing colibactin triggers premature and transmissible senescence in mammalian cells. PLoS ONE. 2013;8:e77157 pubmed publisher
    ..The bacterial genotoxin colibactin, encoded by the pks genomic island is frequently harboured by Escherichia coli strains of the B2 phylogenetic group...
  6. Miajlovic H, Cooke N, Moran G, Rogers T, Smith S. Response of extraintestinal pathogenic Escherichia coli to human serum reveals a protective role for Rcs-regulated exopolysaccharide colanic acid. Infect Immun. 2014;82:298-305 pubmed publisher
    Extraintestinal Escherichia coli (ExPEC) organisms are the leading cause of Gram-negative bacterial bloodstream infections...
  7. Voets G, Fluit A, Scharringa J, Schapendonk C, van den Munckhof T, Leverstein van Hall M, et al. Identical plasmid AmpC beta-lactamase genes and plasmid types in E. coli isolates from patients and poultry meat in the Netherlands. Int J Food Microbiol. 2013;167:359-62 pubmed publisher
    ..that poultry meat and humans share identical Extended-Spectrum Beta-Lactamase genes, plasmid types, and Escherichia coli strain types, suggesting that transmission from poultry meat to humans may occur...
  8. Boedicker J, Garcia H, Johnson S, Phillips R. DNA sequence-dependent mechanics and protein-assisted bending in repressor-mediated loop formation. Phys Biol. 2013;10:066005 pubmed publisher
    ..Such a quantitative understanding of how mechanical regulatory information is encoded in the genome will be a key step towards a predictive understanding of gene regulation at single-base pair resolution. ..
  9. Bakshi S, Dalrymple R, Li W, Choi H, Weisshaar J. Partitioning of RNA polymerase activity in live Escherichia coli from analysis of single-molecule diffusive trajectories. Biophys J. 2013;105:2676-86 pubmed publisher
    Superresolution fluorescence microscopy is used to locate single copies of RNA polymerase (RNAP) in live Escherichia coli and track their diffusive motion. On a timescale of 0...

More Information

Publications103 found, 100 shown here

  1. Bibbal D, Kérourédan M, Loukiadis E, Scheutz F, Oswald E, Brugère H. Slaughterhouse effluent discharges into rivers not responsible for environmental occurrence of enteroaggregative Escherichia coli. Vet Microbiol. 2014;168:451-4 pubmed publisher
    Enteroaggregative Shiga-toxin-producing Escherichia coli strains were responsible for a massive outbreak in Europe in 2011, and had been previously isolated from French patients...
  2. Blank D, Wolf L, Ackermann M, Silander O. The predictability of molecular evolution during functional innovation. Proc Natl Acad Sci U S A. 2014;111:3044-9 pubmed publisher
    ..Here we used experimental evolution with the bacterium Escherichia coli to quantify the molecular changes underlying functional innovation in 68 independent instances ranging over ..
  3. Kim T, Lee J, Kim K. Escherichia coli YmdB regulates biofilm formation independently of its role as an RNase III modulator. BMC Microbiol. 2013;13:266 pubmed publisher
    Ribonuclease III (RNase III) activity modulates hundreds of genes in Escherichia coli (E. coli)...
  4. Salvail H, Caron M, B langer J, Mass E. Antagonistic functions between the RNA chaperone Hfq and an sRNA regulate sensitivity to the antibiotic colicin. EMBO J. 2013;32:2764-78 pubmed publisher
    ..Altogether, these results indicate that Fur and RyhB modulate an unexpected feed-forward loop mechanism related to iron physiology and colicin sensitivity...
  5. Colak G, Xie Z, Zhu A, Dai L, Lu Z, Zhang Y, et al. Identification of lysine succinylation substrates and the succinylation regulatory enzyme CobB in Escherichia coli. Mol Cell Proteomics. 2013;12:3509-20 pubmed publisher
    ..In addition, it is highly likely that lysine succinylation could have unique and more profound regulatory roles in cellular metabolism relative to lysine acetylation under some physiological conditions. ..
  6. Sobota J, Gu M, Imlay J. Intracellular hydrogen peroxide and superoxide poison 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase, the first committed enzyme in the aromatic biosynthetic pathway of Escherichia coli. J Bacteriol. 2014;196:1980-91 pubmed publisher
    In Escherichia coli, aromatic compound biosynthesis is the process that has shown the greatest sensitivity to hydrogen peroxide stress...
  7. Leistner R, Meyer E, Gastmeier P, Pfeifer Y, Eller C, Dem P, et al. Risk factors associated with the community-acquired colonization of extended-spectrum beta-lactamase (ESBL) positive Escherichia Coli. an exploratory case-control study. PLoS ONE. 2013;8:e74323 pubmed publisher
    The number of extended-spectrum beta-lactamase (ESBL) positive (+) Escherichia coli is increasing worldwide. In contrast with many other multidrug-resistant bacteria, it is suspected that they predominantly spread within the community...
  8. Guimaraes J, Rocha M, Arkin A. Transcript level and sequence determinants of protein abundance and noise in Escherichia coli. Nucleic Acids Res. 2014;42:4791-9 pubmed publisher
    ..set of factors explaining 66% of the total variation of protein abundance observed in >800 genes in Escherichia coli. The model suggests that protein abundance is primarily determined by the transcript level (53%) and by ..
  9. Ezemaduka A, Yu J, Shi X, Zhang K, Yin C, Fu X, et al. A small heat shock protein enables Escherichia coli to grow at a lethal temperature of 50°C conceivably by maintaining cell envelope integrity. J Bacteriol. 2014;196:2004-11 pubmed publisher
    It is essential for organisms to adapt to fluctuating growth temperatures. Escherichia coli, a model bacterium commonly used in research and industry, has been reported to grow at a temperature lower than 46.5°C...
  10. Croxen M, Law R, Scholz R, Keeney K, Wlodarska M, Finlay B. Recent advances in understanding enteric pathogenic Escherichia coli. Clin Microbiol Rev. 2013;26:822-80 pubmed publisher
    Although Escherichia coli can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. Pathogenic variants of E...
  11. Sharma P, Stagge S, Bekker M, Bettenbrock K, Hellingwerf K. Kinase activity of ArcB from Escherichia coli is subject to regulation by both ubiquinone and demethylmenaquinone. PLoS ONE. 2013;8:e75412 pubmed publisher
    Expression of the catabolic network in Escherichia coli is predominantly regulated, via oxygen availability, by the two-component system ArcBA...
  12. Seitl I, Wickles S, Beckmann R, Kuhn A, Kiefer D. The C-terminal regions of YidC from Rhodopirellula baltica and Oceanicaulis alexandrii bind to ribosomes and partially substitute for SRP receptor function in Escherichia coli. Mol Microbiol. 2014;91:408-21 pubmed publisher
    The marine Gram-negative bacteria Rhodopirellula baltica and Oceanicaulis alexandrii have, in contrast to Escherichia coli, membrane insertases with extended positively charged C-terminal regions similar to the YidC homologues in ..
  13. Fernandes Filho T, Fávaro C, Ingberman M, Beirão B, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6 pubmed
    Avian pathogenic Escherichia coli is a current problem in the poultry industry, causing mortality and economic losses. This paper evaluates the dynamics in immune response after the use of spray vaccination against E...
  14. Uppal S, Shetty D, Jawali N. Cyclic AMP receptor protein regulates cspD, a bacterial toxin gene, in Escherichia coli. J Bacteriol. 2014;196:1569-77 pubmed publisher
    cspD, a member of cspA family of cold shock genes in Escherichia coli, is not induced during cold shock. Its expression is induced during stationary phase. CspD inhibits DNA replication, and a high level of the protein is toxic to cells...
  15. Plucain J, Hindré T, Le Gac M, Tenaillon O, Cruveiller S, Medigue C, et al. Epistasis and allele specificity in the emergence of a stable polymorphism in Escherichia coli. Science. 2014;343:1366-9 pubmed publisher
    ..We examined how two Escherichia coli lineages diverged from their common ancestor at the outset of a long-term coexistence...
  16. Lema tre C, Mahjoub Messai F, Dupont D, Caro V, Diancourt L, Bingen E, et al. A conserved virulence plasmidic region contributes to the virulence of the multiresistant Escherichia coli meningitis strain S286 belonging to phylogenetic group C. PLoS ONE. 2013;8:e74423 pubmed publisher
    Recent isolation of the non-K1 Escherichia coli neonatal meningitis strain S286, belonging to phylogroup C, which is closely related to major group B1, and producing an extended-spectrum beta-lactamase, encouraged us to seek the genetic ..
  17. Shuja R, Taimuri S, Shakoori F, Shakoori A. Efficient expression of truncated recombinant cadmium-metallothionein gene of a ciliate, Tetrahymena tropicalis lahorensis in Escherichia coli. Mol Biol Rep. 2013;40:7061-8 pubmed publisher
    Truncated recombinant metallothionein GST-fusion protein has been successfully expressed in Escherichia coli. The previously identified novel Cd-inducible metallothionein (TMCd1) gene from the locally isolated ciliate, Tetrahymena ..
  18. Price L, Johnson J, Aziz M, Clabots C, Johnston B, Tchesnokova V, et al. The epidemic of extended-spectrum-?-lactamase-producing Escherichia coli ST131 is driven by a single highly pathogenic subclone, H30-Rx. MBio. 2013;4:e00377-13 pubmed publisher
    The Escherichia coli sequence type 131 (ST131) clone is notorious for extraintestinal infections, fluoroquinolone resistance, and extended-spectrum beta-lactamase (ESBL) production, attributable to a CTX-M-15-encoding mobile element...
  19. Marini G, Luchese M, Argondizzo A, de Góes A, Galler R, Alves T, et al. Experimental design approach in recombinant protein expression: determining medium composition and induction conditions for expression of pneumolysin from Streptococcus pneumoniae in Escherichia coli and preliminary purification process. BMC Biotechnol. 2014;14:1 pubmed publisher
    ..One of the most prominent proteins is pneumolysin (Ply), present in almost all the serotypes known at the moment, which shows an effective protection against S. pneumoniae infections...
  20. Osugui L, de Castro A, Iovine R, Irino K, Carvalho V. Virulence genotypes, antibiotic resistance and the phylogenetic background of extraintestinal pathogenic Escherichia coli isolated from urinary tract infections of dogs and cats in Brazil. Vet Microbiol. 2014;171:242-7 pubmed publisher
    Urinary tract infection (UTI) is a frequent disease of humans and pets and has extra-intestinal pathogenic Escherichia coli (ExPEC) strains as one of the main etiologic agent...
  21. Stringer A, Currenti S, Bonocora R, Baranowski C, Petrone B, Palumbo M, et al. Genome-scale analyses of Escherichia coli and Salmonella enterica AraC reveal noncanonical targets and an expanded core regulon. J Bacteriol. 2014;196:660-71 pubmed publisher
    b>Escherichia coli AraC is a well-described transcription activator of genes involved in arabinose metabolism...
  22. Westra E, Semenova E, Datsenko K, Jackson R, Wiedenheft B, Severinov K, et al. Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition. PLoS Genet. 2013;9:e1003742 pubmed publisher
    ..To determine if the RNA-guide participates in self versus non-self discrimination in the Type I-E system from Escherichia coli we altered base pairing potential between the RNA-guide and the flanks of DNA targets...
  23. Ursell T, NGUYEN J, Monds R, Colavin A, Billings G, Ouzounov N, et al. Rod-like bacterial shape is maintained by feedback between cell curvature and cytoskeletal localization. Proc Natl Acad Sci U S A. 2014;111:E1025-34 pubmed publisher
    ..Precise regulation of rod-like shape in Escherichia coli cells requires the MreB actin-like cytoskeleton, but the mechanism by which MreB maintains rod-like shape is ..
  24. King J, Berry S, Clarke B, Morris R, Whitfield C. Lipopolysaccharide O antigen size distribution is determined by a chain extension complex of variable stoichiometry in Escherichia coli O9a. Proc Natl Acad Sci U S A. 2014;111:6407-12 pubmed publisher
    ..b>Escherichia coli O9a is a prototype for the biosynthesis of O polysaccharides by ATP-binding cassette transporter-dependent ..
  25. Pasom W, Chanawong A, Lulitanond A, Wilailuckana C, Kenprom S, Puang Ngern P. Plasmid-mediated quinolone resistance genes, aac(6')-Ib-cr, qnrS, qnrB, and qnrA, in urinary isolates of Escherichia coli and Klebsiella pneumoniae at a teaching hospital, Thailand. Jpn J Infect Dis. 2013;66:428-32 pubmed
    A total of 121 Escherichia coli (47 extended-spectrum ?-lactamase [ESBL] and 74 non-ESBL producers) and 75 Klebsiella pneumoniae isolates (49 ESBL and 26 non-ESBL producers) were collected from urine samples between October 2010 and April ..
  26. Hernandez J, Johansson A, Stedt J, Bengtsson S, Porczak A, Granholm S, et al. Characterization and comparison of extended-spectrum ?-lactamase (ESBL) resistance genotypes and population structure of Escherichia coli isolated from Franklin's gulls (Leucophaeus pipixcan) and humans in Chile. PLoS ONE. 2013;8:e76150 pubmed publisher
    ..Apart from keeping the antibiotic use low, breaking the transmission chains between the environment and humans must be a priority to hinder the dissemination of resistance. ..
  27. Cambridge J, Blinkova A, Magnan D, Bates D, Walker J. A replication-inhibited unsegregated nucleoid at mid-cell blocks Z-ring formation and cell division independently of SOS and the SlmA nucleoid occlusion protein in Escherichia coli. J Bacteriol. 2014;196:36-49 pubmed publisher
    Chromosome replication and cell division of Escherichia coli are coordinated with growth such that wild-type cells divide once and only once after each replication cycle...
  28. Nicolaes V, El Hajjaji H, Davis R, Van der Henst C, Depuydt M, Leverrier P, et al. Insights into the function of YciM, a heat shock membrane protein required to maintain envelope integrity in Escherichia coli. J Bacteriol. 2014;196:300-9 pubmed publisher
    ..In a search for new envelope assembly factors, we screened a collection of Escherichia coli deletion mutants for sensitivity to detergents and hydrophobic antibiotics, a phenotype indicative of defects ..
  29. Fu Y, Zhou E, Liu Z, Li F, Liang D, Liu B, et al. Staphylococcus aureus and Escherichia coli elicit different innate immune responses from bovine mammary epithelial cells. Vet Immunol Immunopathol. 2013;155:245-52 pubmed publisher
    b>Escherichia coli and Staphylococcus aureus are the most important pathogenic bacteria causing bovine clinical mastitis and subclinical mastitis, respectively...
  30. Cafarelli T, Rands T, Godoy V. The DinB•RecA complex of Escherichia coli mediates an efficient and high-fidelity response to ubiquitous alkylation lesions. Environ Mol Mutagen. 2014;55:92-102 pubmed publisher
    ..However, it is not yet known whether Escherichia coli (E...
  31. van Heeswijk W, Westerhoff H, Boogerd F. Nitrogen assimilation in Escherichia coli: putting molecular data into a systems perspective. Microbiol Mol Biol Rev. 2013;77:628-95 pubmed publisher
    ..of the hierarchical network of intracellular processes revolving around central nitrogen metabolism in Escherichia coli. The hierarchy intertwines transport, metabolism, signaling leading to posttranslational modification, and ..
  32. Taniuchi M, Sobuz S, Begum S, Platts Mills J, Liu J, Yang Z, et al. Etiology of diarrhea in Bangladeshi infants in the first year of life analyzed using molecular methods. J Infect Dis. 2013;208:1794-802 pubmed publisher
    ..Diarrhea causes enormous morbidity and mortality in developing countries, yet the relative importance of multiple potential enteropathogens has been difficult to ascertain...
  33. Behrendt J, Brüser T. The TatBC complex of the Tat protein translocase in Escherichia coli and its transition to the substrate-bound TatABC complex. Biochemistry. 2014;53:2344-54 pubmed publisher
    ..In Escherichia coli, a complex consisting of multiple copies of TatB and TatC initiates the transport by binding the signal ..
  34. Dassama L, Silakov A, Krest C, Calixto J, Krebs C, Bollinger J, et al. A 2.8 Å Fe-Fe separation in the Fe2(III/IV) intermediate, X, from Escherichia coli ribonucleotide reductase. J Am Chem Soc. 2013;135:16758-61 pubmed publisher
    ..During self-assembly of the Escherichia coli RNR-? cofactor, reaction of the protein's Fe2(II/II) complex with O2 results in accumulation of an Fe2(III/IV)..
  35. Cao G, Zeng X, Chen A, Zhou L, Zhang L, Xiao Y, et al. Effects of a probiotic, Enterococcus faecium, on growth performance, intestinal morphology, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88. Poult Sci. 2013;92:2949-55 pubmed publisher
    ..faecium on growth performance, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88 were investigated...
  36. Skibinski D, Hanson B, Lin Y, von Messling V, Jegerlehner A, Tee J, et al. Enhanced neutralizing antibody titers and Th1 polarization from a novel Escherichia coli derived pandemic influenza vaccine. PLoS ONE. 2013;8:e76571 pubmed publisher
    ..In ferrets the vaccine elicited neutralizing antibodies, and following challenge with influenza A/California/07/2009 virus reduced morbidity and lowered viral titers in nasal lavages. ..
  37. Clatterbuck Soper S, Dator R, Limbach P, Woodson S. In vivo X-ray footprinting of pre-30S ribosomes reveals chaperone-dependent remodeling of late assembly intermediates. Mol Cell. 2013;52:506-16 pubmed publisher
    ..Our results indicate that cellular factors delay specific RNA folding steps to ensure the quality of assembly...
  38. Kiro R, Molshanski Mor S, Yosef I, Milam S, Erickson H, Qimron U. Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division. Proc Natl Acad Sci U S A. 2013;110:19549-54 pubmed publisher
    ..One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that Gp0.4 is a direct inhibitor of the E...
  39. Han X, Parkinson J. An unorthodox sensory adaptation site in the Escherichia coli serine chemoreceptor. J Bacteriol. 2014;196:641-9 pubmed publisher
    The serine chemoreceptor of Escherichia coli contains four canonical methylation sites for sensory adaptation that lie near intersubunit helix interfaces of the Tsr homodimer...
  40. Pande S, Merker H, Bohl K, Reichelt M, Schuster S, de Figueiredo L, et al. Fitness and stability of obligate cross-feeding interactions that emerge upon gene loss in bacteria. ISME J. 2014;8:953-62 pubmed publisher
    ..problem using synthetic cross-feeding interactions: by simply deleting two metabolic genes from the genome of Escherichia coli, we generated genotypes that require amino acids to grow and release other amino acids into the environment...
  41. Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. J Immunol. 2014;192:804-16 pubmed publisher
    A Salmonella therapeutic expressing enterotoxigenic Escherichia coli colonization factor Ag I (CFA/I) fimbriae protects against collagen-induced arthritis (CIA) by eliciting two regulatory T cell (Treg) subsets: TGF-?-producing Foxp3(-)..
  42. Banerjee R, Robicsek A, Kuskowski M, Porter S, Johnston B, Sokurenko E, et al. Molecular epidemiology of Escherichia coli sequence type 131 and Its H30 and H30-Rx subclones among extended-spectrum-?-lactamase-positive and -negative E. coli clinical isolates from the Chicago Region, 2007 to 2010. Antimicrob Agents Chemother. 2013;57:6385-8 pubmed publisher
    We assessed Escherichia coli ST131 and its H30 and H30-Rx subclones for virulence genes, antimicrobial resistance, and extended-spectrum beta-lactamase (ESBL) type...
  43. Manabe T, Kato M, Ueno T, Kawasaki K. Flagella proteins contribute to the production of outer membrane vesicles from Escherichia coli W3110. Biochem Biophys Res Commun. 2013;441:151-6 pubmed publisher
    Gram-negative bacteria, including Escherichia coli, release outer membrane vesicles (OMVs) that are derived from the bacterial outer membrane...
  44. Oldham M, Chen S, Chen J. Structural basis for substrate specificity in the Escherichia coli maltose transport system. Proc Natl Acad Sci U S A. 2013;110:18132-7 pubmed publisher
    ..For the Escherichia coli maltose transport system, the selectivity of sugar binding to maltose-binding protein (MBP), the periplasmic ..
  45. Li X, Thomason L, Sawitzke J, Costantino N, Court D. Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli. Nucleic Acids Res. 2013;41:e204 pubmed publisher
    ..These two genes are introduced as a joint DNA cassette into Escherichia coli by selection for Tc(R)...
  46. Bae B, Davis E, Brown D, Campbell E, Wigneshweraraj S, Darst S. Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of ?70 domain 1.1. Proc Natl Acad Sci U S A. 2013;110:19772-7 pubmed publisher
    Bacteriophage T7 encodes an essential inhibitor of the Escherichia coli host RNA polymerase (RNAP), the product of gene 2 (Gp2). We determined a series of X-ray crystal structures of E. coli RNAP holoenzyme with or without Gp2...
  47. Duval M, Korepanov A, Fuchsbauer O, Fechter P, Haller A, Fabbretti A, et al. Escherichia coli ribosomal protein S1 unfolds structured mRNAs onto the ribosome for active translation initiation. PLoS Biol. 2013;11:e1001731 pubmed publisher
    ..This work shows that S1 confers to the ribosome dynamic properties to initiate translation of a large set of mRNAs with diverse structural features. ..
  48. Deshmukh H, Liu Y, Menkiti O, Mei J, Dai N, O Leary C, et al. The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice. Nat Med. 2014;20:524-30 pubmed publisher
    ..Granulocytopenia was associated with impaired host defense and increased susceptibility to Escherichia coli K1 and Klebsiella pneumoniae sepsis in antibiotic-treated neonates, which could be partially reversed by ..
  49. Li G, Burkhardt D, Gross C, Weissman J. Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell. 2014;157:624-35 pubmed publisher
    ..Our studies reveal how general principles, important both for understanding natural systems and for synthesizing new ones, emerge from quantitative analyses of protein synthesis. ..
  50. Peer A, Margalit H. Evolutionary patterns of Escherichia coli small RNAs and their regulatory interactions. RNA. 2014;20:994-1003 pubmed publisher
    ..Here we used the prokaryotic phylogenetic tree to reconstruct the evolutionary history of Escherichia coli sRNAs and their binding sites on target mRNAs. We discovered that sRNAs currently present in E...
  51. Ruiz Pernía J, Luk L, García Meseguer R, Martí S, Loveridge E, Tuñón I, et al. Increased dynamic effects in a catalytically compromised variant of Escherichia coli dihydrofolate reductase. J Am Chem Soc. 2013;135:18689-96 pubmed publisher
    Isotopic substitution ((15)N, (13)C, (2)H) of a catalytically compromised variant of Escherichia coli dihydrofolate reductase, EcDHFR-N23PP/S148A, has been used to investigate the effect of these mutations on catalysis...
  52. Nielsen K, Dynesen P, Larsen P, Jakobsen L, Andersen P, Frimodt Møller N. Role of urinary cathelicidin LL-37 and human ?-defensin 1 in uncomplicated Escherichia coli urinary tract infections. Infect Immun. 2014;82:1572-8 pubmed publisher
    ..The aim of this study was to characterize whether these peptides are important for developing uncomplicated Escherichia coli urinary tract infections (UTIs)...
  53. Lybecker M, Zimmermann B, Bilusic I, Tukhtubaeva N, Schroeder R. The double-stranded transcriptome of Escherichia coli. Proc Natl Acad Sci U S A. 2014;111:3134-9 pubmed publisher
    ..Total RNA and immunoprecipitated dsRNA from Escherichia coli RNase III WT and mutant strains were deep-sequenced...
  54. Bednorz C, Guenther S, Oelgeschläger K, Kinnemann B, Pieper R, Hartmann S, et al. Feeding the probiotic Enterococcus faecium strain NCIMB 10415 to piglets specifically reduces the number of Escherichia coli pathotypes that adhere to the gut mucosa. Appl Environ Microbiol. 2013;79:7896-904 pubmed publisher
    ..Since Escherichia coli is among the most important pathogens in pig production, we performed comprehensive analyses to gain further ..
  55. Gan H, Sieo C, Tang S, Omar A, Ho Y. The complete genome sequence of EC1-UPM, a novel N4-like bacteriophage that infects Escherichia coli O78:K80. Virol J. 2013;10:308 pubmed publisher
    Bacteriophage EC1-UPM is an N4-like bacteriophage which specifically infects Escherichia coli O78:K80, an avian pathogenic strain that causes colibacillosis in poultry...
  56. Madar D, Dekel E, Bren A, Zimmer A, Porat Z, Alon U. Promoter activity dynamics in the lag phase of Escherichia coli. BMC Syst Biol. 2013;7:136 pubmed publisher
    ..However, lag phase has important implications for bacterial infections and food safety. We asked which, if any, genes are expressed in the lag phase of Escherichia coli, and what is their dynamic expression pattern.
  57. Izquierdo M, Navarro Garcia F, Nava Acosta R, Nataro J, Ruiz Perez F, Farfan M. Identification of cell surface-exposed proteins involved in the fimbria-mediated adherence of enteroaggregative Escherichia coli to intestinal cells. Infect Immun. 2014;82:1719-24 pubmed publisher
    Fimbria-mediated adherence to the intestinal epithelia is a key step in enteroaggregative Escherichia coli (EAEC) pathogenesis...
  58. Parks A, Court C, Lubkowska L, Jin D, Kashlev M, Court D. Bacteriophage ? N protein inhibits transcription slippage by Escherichia coli RNA polymerase. Nucleic Acids Res. 2014;42:5823-9 pubmed publisher
    ..This report provides the first evidence of a protein that directly influences transcriptional slippage, and provides a clue about the molecular mechanism of transcription termination and N-mediated antitermination. ..
  59. Guo Y, Quiroga C, Chen Q, McAnulty M, Benedik M, Wood T, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42:6448-62 pubmed publisher
    ..Also, RalR/RalA is beneficial to the Escherichia coli host for responding to the antibiotic fosfomycin...
  60. Parker B, Schwessinger E, Jakob U, Gray M. The RclR protein is a reactive chlorine-specific transcription factor in Escherichia coli. J Biol Chem. 2013;288:32574-84 pubmed publisher
    ..We have now used in vivo genetic and in vitro biochemical methods to identify and demonstrate that Escherichia coli RclR (formerly YkgD) is a redox-regulated transcriptional activator of the AraC family, whose highly ..
  61. Forster S, Brandt M, Mottok D, Zschüttig A, Zimmermann K, Blattner F, et al. Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct. BMC Biotechnol. 2013;13:82 pubmed publisher
    ..construct was designed for secretory expression of recombinant viral IL-10 proteins in the periplasm of Escherichia coli laboratory strain BL21 (DE3), which might serve as part of a prospective lysis based delivery and containment ..
  62. Keren L, Zackay O, Lotan Pompan M, Barenholz U, Dekel E, Sasson V, et al. Promoters maintain their relative activity levels under different growth conditions. Mol Syst Biol. 2013;9:701 pubmed publisher
    ..It suggests that many changes in expression across conditions result from global effects and not specific regulation, and provides means for quantitative interpretation of expression profiles...
  63. Salscheider S, Jahn A, Schnetz K. Transcriptional regulation by BglJ-RcsB, a pleiotropic heteromeric activator in Escherichia coli. Nucleic Acids Res. 2014;42:2999-3008 pubmed publisher
    ..Thus, BglJ-RcsB is a pleiotropic transcriptional activator that coordinates regulation with global regulators including CRP, LeuO and HNS. ..
  64. Hoch P, Hartmann R. Supramolecular membrane-associated assemblies of RNA metabolic proteins in Escherichia coli. Biochem J. 2014;458:e1-3 pubmed publisher
    ..In this issue of the Biochemical Journal, Taghbalout et al. demonstrate in Escherichia coli that many additional proteins of the RNA degradation and processing network co-localize with the RNA ..
  65. Rubio D, Nebot E, Casanueva J, Pulgarin C. Comparative effect of simulated solar light, UV, UV/H2O2 and photo-Fenton treatment (UV-Vis/H2O2/Fe2+,3+) in the Escherichia coli inactivation in artificial seawater. Water Res. 2013;47:6367-79 pubmed publisher
    ..In Milli-Q water, the rate of inactivation for Escherichia coli was higher than in Leman Lake water and seawater due to the lack of inorganic ions affecting negatively ..
  66. Monzel C, Degreif Dünnwald P, Gröpper C, Griesinger C, Unden G. The cytoplasmic PASC domain of the sensor kinase DcuS of Escherichia coli: role in signal transduction, dimer formation, and DctA interaction. Microbiologyopen. 2013;2:912-27 pubmed publisher
    The cytoplasmic PASC domain of the fumarate responsive sensor kinase DcuS of Escherichia coli links the transmembrane to the kinase domain. PASC is also required for interaction with the transporter DctA serving as a cosensor of DcuS...
  67. Zhou Y, Nambou K, Wei L, Cao J, Imanaka T, Hua Q. Lycopene production in recombinant strains of Escherichia coli is improved by knockout of the central carbon metabolism gene coding for glucose-6-phosphate dehydrogenase. Biotechnol Lett. 2013;35:2137-45 pubmed publisher
    ..was undertaken in order to understand the mechanism involved in the heterologous synthesis of lycopene in Escherichia coli. Knockout of the central carbon metabolic gene zwf (glucose-6-phosphate dehydrogenase) resulted in the ..
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    The histidine autokinase CheA functions as the central processing unit in the Escherichia coli chemotaxis signaling machinery...
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    Chicken products represent a source for multidrug-resistant Escherichia coli causing extraintestinal infections (ExPEC) in humans. We applied phylogenetic analysis to a collection of E...
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    ..In this article we review these studies and the emerging ideas in this field. This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey. ..
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    ..Sulfated Escherichia coli K5 derivatives, which are structurally similar to heparin/heparan sulfate but lack anticoagulant activity, ..
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    ..Here, by studying mYFP-tagged sensory kinases in Escherichia coli, we show that the tagged TorS and EvgS sensors have a clear tendency for self-association and clustering...
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    ..auspices of the Spanish Society for Infectious Diseases and Clinical Microbiology Quality Control program, 14 Escherichia coli strains masked as blood culture isolates were sent to 68 clinical microbiology laboratories for antimicrobial ..
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    ..The results showed superior properties and synergistic antibacterial effects by combining chitosan with Ag-NPs...
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    ..coli-expressed Protein A-mouse AMCase-V5-His fusion protein possesses chitinase functions comparable to the CHO-expressed AMCase. This recombinant protein can be used to elucidate detailed biomedical functions of the mouse AMCase. ..
  77. Dudin O, Geiselmann J, Ogasawara H, Ishihama A, Lacour S. Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation. J Bacteriol. 2014;196:707-15 pubmed publisher
    b>Escherichia coli adapts its lifestyle to the variations of environmental growth conditions, swapping between swimming motility or biofilm formation...
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    Genome-scale models (GEMs) of metabolism were constructed for 55 fully sequenced Escherichia coli and Shigella strains. The GEMs enable a systems approach to characterizing the pan and core metabolic capabilities of the E. coli species...
  79. Pimentel B, Nair R, Bermejo Rodríguez C, Preston M, Agu C, Wang X, et al. Toxin Kid uncouples DNA replication and cell division to enforce retention of plasmid R1 in Escherichia coli cells. Proc Natl Acad Sci U S A. 2014;111:2734-9 pubmed publisher
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    ..We propose that, in Escherichia coli, such a gradient of membrane tethering sites may be provided by the oscillatory Min system, otherwise known ..
  81. Maharjan R, Ferenci T. Epistatic interactions determine the mutational pathways and coexistence of lineages in clonal Escherichia coli populations. Evolution. 2013;67:2762-8 pubmed publisher
    ..mglD, malT, and hfq present in two coexisting lineages that repeatedly fixed in experimental populations of Escherichia coli. The mutations were transferred into the ancestral background individually or in combination of double or ..
  82. Lin C, Awano N, Masuda H, Park J, Inouye M. Transcriptional repressor HipB regulates the multiple promoters in Escherichia coli. J Mol Microbiol Biotechnol. 2013;23:440-7 pubmed publisher
    HipB is a DNA-binding protein in Escherichia coli and negatively regulates its own promoter by binding to the palindromic sequences [TATCCN8GGATA (N represents any nucleotides)] on the hipBA promoter...
  83. Kim J, Nakagawa A, Yamazaki Y, Matsumura E, Koyanagi T, Minami H, et al. Improvement of reticuline productivity from dopamine by using engineered Escherichia coli. Biosci Biotechnol Biochem. 2013;77:2166-8 pubmed
    ..We have previously devised a reticuline (BIA) production method from dopamine by using Escherichia coli; however, its productivity was relatively low (33 µM, 11 mg/L)...
  84. Salomonsen C, Boye M, Høiby N, Jensen T, Hammer A. Comparison of histological lesions in mink with acute hemorrhagic pneumonia associated with Pseudomonas aeruginosa or Escherichia coli. Can J Vet Res. 2013;77:199-204 pubmed
    ..In recent years, however, outbreaks of this type of pneumonia that are associated with hemolytic Escherichia coli have also occurred in farmed mink...
  85. Pons M, Mosquito S, Gomes C, del Valle L, Ochoa T, Ruiz J. Analysis of quinolone-resistance in commensal and diarrheagenic Escherichia coli isolates from infants in Lima, Peru. Trans R Soc Trop Med Hyg. 2014;108:22-8 pubmed publisher
    ..The aim of this study was to analyse the prevalence of the molecular mechanisms of quinolone-resistance in E. coli isolated from faeces of healthy Peruvian children or those presenting diarrhoea...
  86. Wacker M, Wang L, Kowarik M, Dowd M, Lipowsky G, Faridmoayer A, et al. Prevention of Staphylococcus aureus infections by glycoprotein vaccines synthesized in Escherichia coli. J Infect Dis. 2014;209:1551-61 pubmed publisher
    ..Vaccines have the potential to reduce the morbidity, mortality, and economic impact associated with staphylococcal infections. However, single-component vaccines targeting S. aureus have failed to show efficacy in clinical trials...
  87. Lesterlin C, Ball G, Schermelleh L, Sherratt D. RecA bundles mediate homology pairing between distant sisters during DNA break repair. Nature. 2014;506:249-53 pubmed publisher using conventional and super-resolution microscopy, we describe the progression of DSB repair in live Escherichia coli. Specifically, we investigate whether homologous recombination can occur efficiently between distant sister ..
  88. Song W, Kim Y, Sim S, Hwang S, Lee J, Lee Y, et al. Antibiotic stress-induced modulation of the endoribonucleolytic activity of RNase III and RNase G confers resistance to aminoglycoside antibiotics in Escherichia coli. Nucleic Acids Res. 2014;42:4669-81 pubmed publisher
    ..mechanism that is induced through the modulation of 16S ribosomal RNA (rRNA) processing on the exposure of Escherichia coli cells to aminoglycoside antibiotics...
  89. Kleta S, Nordhoff M, Tedin K, Wieler L, Kolenda R, Oswald S, et al. Role of F1C fimbriae, flagella, and secreted bacterial components in the inhibitory effect of probiotic Escherichia coli Nissle 1917 on atypical enteropathogenic E. coli infection. Infect Immun. 2014;82:1801-12 pubmed publisher
    Enteropathogenic Escherichia coli (EPEC) is recognized as an important intestinal pathogen that frequently causes acute and persistent diarrhea in humans and animals...
  90. Mazurek J, Pusz P, Bok E, Stosik M, Baldy Chudzik K. The phenotypic and genotypic characteristics of antibiotic resistance in Escherichia coli populations isolated from farm animals with different exposure to antimicrobial agents. Pol J Microbiol. 2013;62:173-9 pubmed
    ..29%), bla(TEM) (0.43%) and tetC (0.43%). The presented outcomes provide evidence that antimicrobial pressure contributes to resistance development, and enteric microflora constitutes an essential reservoir of resistance genes...
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    ..Recent advances in metal ion-catalyzed peptide bond cleavage allow for more mild conditions, and some methods involving nickel or palladium will likely soon appear in more biological applications. ..
  92. Youngquist J, Schumacher M, Rose J, Raines T, Politz M, Copeland M, et al. Production of medium chain length fatty alcohols from glucose in Escherichia coli. Metab Eng. 2013;20:177-86 pubmed publisher
    ..ligase (FadD), and an acyl-CoA/aldehyde reductase (MAACR) were overexpressed in an engineered strain of Escherichia coli. Yields were improved by balancing expression levels of each gene, using a fed-batch cultivation strategy, ..