caulobacter crescentus

Summary

Summary: A species of gram-negative, aerobic bacteria that consist of slender vibroid cells.

Top Publications

  1. Gitai Z, Dye N, Reisenauer A, Wachi M, Shapiro L. MreB actin-mediated segregation of a specific region of a bacterial chromosome. Cell. 2005;120:329-41 pubmed
    ..MreB selectively interacts, directly or indirectly, with origin-proximal regions of the chromosome, arguing that the origin-proximal region segregates via an MreB-dependent mechanism not used by the rest of the chromosome. ..
  2. Wang S, West L, Shapiro L. The bifunctional FtsK protein mediates chromosome partitioning and cell division in Caulobacter. J Bacteriol. 2006;188:1497-508 pubmed publisher
    ..We show here that the Caulobacter crescentus FtsK protein localizes to the division plane, where it mediates multiple functions involved in chromosome ..
  3. Reisinger S, Huntwork S, Viollier P, Ryan K. DivL performs critical cell cycle functions in Caulobacter crescentus independent of kinase activity. J Bacteriol. 2007;189:8308-20 pubmed
    ..Our results show that DivL controls two key cell cycle regulators, CtrA and DivK, and that phosphoryl transfer is not DivL's essential cellular activity. ..
  4. Bastedo D, Marczynski G. CtrA response regulator binding to the Caulobacter chromosome replication origin is required during nutrient and antibiotic stress as well as during cell cycle progression. Mol Microbiol. 2009;72:139-54 pubmed publisher
    The Caulobacter crescentus chromosome replication origin (Cori) has five binding sites for CtrA, an OmpR/PhoB family 'response regulator'...
  5. Hong S, Tran Q, Keiler K. Cell cycle-regulated degradation of tmRNA is controlled by RNase R and SmpB. Mol Microbiol. 2005;57:565-75 pubmed publisher
    ..in all bacteria, is cell cycle regulated and is important for control of cell cycle progression in Caulobacter crescentus. We report that RNase R, a highly conserved 3' to 5' exoribonuclease, is required for the selective ..
  6. Hocking J, Priyadarshini R, Takacs C, Costa T, Dye N, Shapiro L, et al. Osmolality-dependent relocation of penicillin-binding protein PBP2 to the division site in Caulobacter crescentus. J Bacteriol. 2012;194:3116-27 pubmed publisher
    ..Here we show that the spatial distributions of specific cell wall proteins in Caulobacter crescentus are sensitive to small external osmotic upshifts...
  7. Werner J, Chen E, Guberman J, Zippilli A, Irgon J, Gitai Z. Quantitative genome-scale analysis of protein localization in an asymmetric bacterium. Proc Natl Acad Sci U S A. 2009;106:7858-63 pubmed publisher
    ..We identify nearly 300 localized Caulobacter crescentus proteins, up to 10-fold more than were previously characterized...
  8. Li G, Tang J. Accumulation of microswimmers near a surface mediated by collision and rotational Brownian motion. Phys Rev Lett. 2009;103:078101 pubmed
    ..Simulations based on this model reproduce the density distributions measured for the small bacteria E. coli and Caulobacter crescentus, as well as for the much larger bull spermatozoa swimming between two walls.
  9. Jonas K, Chen Y, Laub M. Modularity of the bacterial cell cycle enables independent spatial and temporal control of DNA replication. Curr Biol. 2011;21:1092-101 pubmed publisher
    ..govern the temporal and spatial control of DNA replication in the asymmetrically dividing bacterium Caulobacter crescentus. DNA replication control involves DnaA, which promotes initiation, and CtrA, which silences initiation...

More Information

Publications114 found, 100 shown here

  1. Viollier P, Shapiro L. A lytic transglycosylase homologue, PleA, is required for the assembly of pili and the flagellum at the Caulobacter crescentus cell pole. Mol Microbiol. 2003;49:331-45 pubmed
    ..In support of this, PleA was found to be present only during a short interval in the cell cycle that coincides with the assembly of the flagellum and the pilus secretion apparatus...
  2. Bodenmiller D, Toh E, Brun Y. Development of surface adhesion in Caulobacter crescentus. J Bacteriol. 2004;186:1438-47 pubmed
    b>Caulobacter crescentus has a dimorphic life cycle composed of a motile stage and a sessile stage. In the sessile stage, C. crescentus is often found tightly attached to a surface through its adhesive holdfast...
  3. Jensen R. Coordination between chromosome replication, segregation, and cell division in Caulobacter crescentus. J Bacteriol. 2006;188:2244-53 pubmed
    Progression through the Caulobacter crescentus cell cycle is coupled to a cellular differentiation program. The swarmer cell is replicationally quiescent, and DNA replication initiates at the swarmer-to-stalked cell transition...
  4. Alvarez Martinez C, Lourenço R, Baldini R, Laub M, Gomes S. The ECF sigma factor sigma(T) is involved in osmotic and oxidative stress responses in Caulobacter crescentus. Mol Microbiol. 2007;66:1240-55 pubmed
    Sigma factors of the ECF subfamily are important regulators of stress responses in bacteria. Analysis of Caulobacter crescentus genome sequence has indicated the presence of 13 members of the ECF (extracytoplasmic function) subfamily, ..
  5. Sciochetti S, Ohta N, Newton A. The role of polar localization in the function of an essential Caulobacter crescentus tyrosine kinase. Mol Microbiol. 2005;56:1467-80 pubmed publisher
    DivL is an essential tyrosine kinase in Caulobacter crescentus that controls an early step in the cell division cycle...
  6. Biondi E, Skerker J, Arif M, Prasol M, Perchuk B, Laub M. A phosphorelay system controls stalk biogenesis during cell cycle progression in Caulobacter crescentus. Mol Microbiol. 2006;59:386-401 pubmed
    ..In Caulobacter crescentus, each cell cycle produces morphologically distinct daughter cells, a stalked cell and a flagellated ..
  7. Russell J, Keiler K. Peptide signals encode protein localization. J Bacteriol. 2007;189:7581-5 pubmed
  8. Christen B, Abeliuk E, Collier J, Kalogeraki V, Passarelli B, Coller J, et al. The essential genome of a bacterium. Mol Syst Biol. 2011;7:528 pubmed publisher
    b>Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle...
  9. Murray S, Panis G, Fumeaux C, Viollier P, Howard M. Computational and genetic reduction of a cell cycle to its simplest, primordial components. PLoS Biol. 2013;11:e1001749 pubmed publisher
    ..In the Alphaproteobacterium Caulobacter crescentus, cell cycle progression is believed to be controlled by a cyclical genetic circuit comprising four ..
  10. Skerker J, Laub M. Cell-cycle progression and the generation of asymmetry in Caulobacter crescentus. Nat Rev Microbiol. 2004;2:325-37 pubmed
  11. Aaron M, Charbon G, Lam H, Schwarz H, Vollmer W, Jacobs Wagner C. The tubulin homologue FtsZ contributes to cell elongation by guiding cell wall precursor synthesis in Caulobacter crescentus. Mol Microbiol. 2007;64:938-52 pubmed
    ..Here, we show that in Caulobacter crescentus, FtsZ also plays a major role in cell elongation by spatially regulating the location of MurG, which ..
  12. Thanbichler M, Iniesta A, Shapiro L. A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus. Nucleic Acids Res. 2007;35:e137 pubmed
    b>Caulobacter crescentus is widely used as a powerful model system for the study of prokaryotic cell biology and development...
  13. England J, Perchuk B, Laub M, Gober J. Global regulation of gene expression and cell differentiation in Caulobacter crescentus in response to nutrient availability. J Bacteriol. 2010;192:819-33 pubmed publisher
    In a developmental strategy designed to efficiently exploit and colonize sparse oligotrophic environments, Caulobacter crescentus cells divide asymmetrically, yielding a motile swarmer cell and a sessile stalked cell...
  14. Radhakrishnan S, Pritchard S, Viollier P. Coupling prokaryotic cell fate and division control with a bifunctional and oscillating oxidoreductase homolog. Dev Cell. 2010;18:90-101 pubmed publisher
    ..NAD(H)-binding regulator (KidO) that integrates cell-fate signaling with cytokinesis in the bacterium Caulobacter crescentus. KidO stimulates the DivJ kinase and directly acts on the cytokinetic tubulin, FtsZ, to tune cytokinesis ..
  15. Fischer B, Rummel G, Aldridge P, Jenal U. The FtsH protease is involved in development, stress response and heat shock control in Caulobacter crescentus. Mol Microbiol. 2002;44:461-78 pubmed
    The ftsH gene of Caulobacter crescentus has been isolated and identified as a component of the general stress response of this organism. In C...
  16. Braz V, da Silva Neto J, Italiani V, Marques M. CztR, a LysR-type transcriptional regulator involved in zinc homeostasis and oxidative stress defense in Caulobacter crescentus. J Bacteriol. 2010;192:5480-8 pubmed publisher
    b>Caulobacter crescentus is a free-living alphaproteobacterium that has 11 predicted LysR-type transcriptional regulators (LTTRs). Previously, a C...
  17. Braz V, Marques M. Genes involved in cadmium resistance in Caulobacter crescentus. FEMS Microbiol Lett. 2005;251:289-95 pubmed
    A transposon library of 5700 mutants was constructed in Caulobacter crescentus strain NA1000...
  18. Collier J, Shapiro L. Feedback control of DnaA-mediated replication initiation by replisome-associated HdaA protein in Caulobacter. J Bacteriol. 2009;191:5706-16 pubmed publisher
    Chromosome replication in Caulobacter crescentus is tightly regulated to ensure that initiation occurs at the right time and only once during the cell cycle. The timing of replication initiation is controlled by both CtrA and DnaA...
  19. Mazzon R, Lang E, Silva C, Marques M. Cold shock genes cspA and cspB from Caulobacter crescentus are posttranscriptionally regulated and important for cold adaptation. J Bacteriol. 2012;194:6507-17 pubmed publisher
    ..b>Caulobacter crescentus has four CSPs: CspA and CspB, which are cold induced, and CspC and CspD, which are induced only in ..
  20. Gitai Z, Dye N, Shapiro L. An actin-like gene can determine cell polarity in bacteria. Proc Natl Acad Sci U S A. 2004;101:8643-8 pubmed
    ..We find that the actin-like MreB protein mediates global cell polarity in Caulobacter crescentus, although the intermediate filament-like CreS protein influences cell shape without affecting cell ..
  21. Skerker J, Prasol M, Perchuk B, Biondi E, Laub M. Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis. PLoS Biol. 2005;3:e334 pubmed
    ..Here we report a system-level investigation of two-component pathways in the model organism Caulobacter crescentus. First, by a comprehensive deletion analysis we show that at least 39 of the 106 two-component genes are ..
  22. Bowman G, Comolli L, Zhu J, Eckart M, Koenig M, Downing K, et al. A polymeric protein anchors the chromosomal origin/ParB complex at a bacterial cell pole. Cell. 2008;134:945-55 pubmed publisher
    ..We have identified a proline-rich polar protein, PopZ, required to anchor the separated Caulobacter crescentus chromosome origins at the cell poles, a function that is essential for maintaining chromosome organization ..
  23. Boutte C, Henry J, Crosson S. ppGpp and polyphosphate modulate cell cycle progression in Caulobacter crescentus. J Bacteriol. 2012;194:28-35 pubmed publisher
    b>Caulobacter crescentus differentiates from a motile, foraging swarmer cell into a sessile, replication-competent stalked cell during its cell cycle...
  24. Koval S, Hynes S, Flannagan R, Pasternak Z, Davidov Y, Jurkevitch E. Bdellovibrio exovorus sp. nov., a novel predator of Caulobacter crescentus. Int J Syst Evol Microbiol. 2013;63:146-51 pubmed publisher
    ..Strain JSS(T) was isolated from sewage in London, Ontario, Canada, in enrichment culture with Caulobacter crescentus prey cells. During predation, this strain remained attached to the outside of a stalked C. crescentus cell...
  25. Hu P, Brodie E, Suzuki Y, McAdams H, Andersen G. Whole-genome transcriptional analysis of heavy metal stresses in Caulobacter crescentus. J Bacteriol. 2005;187:8437-49 pubmed
    The bacterium Caulobacter crescentus and related stalk bacterial species are known for their distinctive ability to live in low-nutrient environments, a characteristic of most heavy metal-contaminated sites...
  26. Cheng L, Keiler K. Correct timing of dnaA transcription and initiation of DNA replication requires trans translation. J Bacteriol. 2009;191:4268-75 pubmed publisher
    ..b>Caulobacter crescentus mutants that lack the trans translation pathway have a defect in the cell cycle and do not initiate DNA ..
  27. Kirkpatrick C, Viollier P. Decoding Caulobacter development. FEMS Microbiol Rev. 2012;36:193-205 pubmed publisher
    b>Caulobacter crescentus uses a multi-layered system of oscillating regulators to program different developmental fates into each daughter cell at division...
  28. Viollier P, Thanbichler M, McGrath P, West L, Meewan M, McAdams H, et al. Rapid and sequential movement of individual chromosomal loci to specific subcellular locations during bacterial DNA replication. Proc Natl Acad Sci U S A. 2004;101:9257-62 pubmed
    ..We examined the cellular position of 112 individual loci that are dispersed over the circular Caulobacter crescentus chromosome and found that in living cells each locus has a specific subcellular address and that these ..
  29. Wagner J, Setayeshgar S, Sharon L, Reilly J, Brun Y. A nutrient uptake role for bacterial cell envelope extensions. Proc Natl Acad Sci U S A. 2006;103:11772-7 pubmed
    ..We show that stalks (prosthecae), cylindrical extensions of the Caulobacter crescentus cell envelope, can take up and hydrolyze organic phosphate molecules and contain the high-affinity ..
  30. Sliusarenko O, Heinritz J, Emonet T, Jacobs Wagner C. High-throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio-temporal dynamics. Mol Microbiol. 2011;80:612-27 pubmed publisher
    ..MicrobeTracker was also used to uncover novel aspects of morphogenesis and cell cycle regulation in Caulobacter crescentus. By tracking filamentous cells, we show that the chromosomal origin at the old-pole is responsible for ..
  31. Modell J, Hopkins A, Laub M. A DNA damage checkpoint in Caulobacter crescentus inhibits cell division through a direct interaction with FtsW. Genes Dev. 2011;25:1328-43 pubmed publisher
    ..Here, we show that Caulobacter crescentus responds to DNA damage by coordinately inducing an SOS regulon and inhibiting the master regulator CtrA...
  32. Collier J. Regulation of chromosomal replication in Caulobacter crescentus. Plasmid. 2012;67:76-87 pubmed publisher
    The alpha-proteobacterium Caulobacter crescentus is characterized by its asymmetric cell division, which gives rise to a replicating stalked cell and a non-replicating swarmer cell...
  33. Lam H, Matroule J, Jacobs Wagner C. The asymmetric spatial distribution of bacterial signal transduction proteins coordinates cell cycle events. Dev Cell. 2003;5:149-59 pubmed
    ..Study of a DivK homolog in the morphologically symmetric bacterium Sinorhizobium meliloti suggests that this type of cell cycle mechanism is widespread in prokaryotes. ..
  34. Siegal Gaskins D, Crosson S. Tightly regulated and heritable division control in single bacterial cells. Biophys J. 2008;95:2063-72 pubmed publisher
    The robust surface adherence property of the aquatic bacterium Caulobacter crescentus permits visualization of single cells in a linear microfluidic culture chamber over an extended number of generations...
  35. Christen B, Fero M, Hillson N, BOWMAN G, Hong S, Shapiro L, et al. High-throughput identification of protein localization dependency networks. Proc Natl Acad Sci U S A. 2010;107:4681-6 pubmed publisher
    ..for polar organelle development, establishment of asymmetry, and chromosome replication during the Caulobacter crescentus cell cycle...
  36. Kohler C, Lourenço R, Avelar G, Gomes S. Extracytoplasmic function (ECF) sigma factor ?F is involved in Caulobacter crescentus response to heavy metal stress. BMC Microbiol. 2012;12:210 pubmed publisher
    The ?-proteobacterium Caulobacter crescentus inhabits low-nutrient environments and can tolerate certain levels of heavy metals in these sites. It has been reported that C...
  37. Quardokus E, Brun Y. Cell cycle timing and developmental checkpoints in Caulobacter crescentus. Curr Opin Microbiol. 2003;6:541-9 pubmed
    ..These signal transduction networks achieve precise timing of the cell cycle and development by regulating temporal gene expression, and protein activity by dynamic spatial localization within the cell and timed proteolysis. ..
  38. Pierce D, O Donnol D, Allen R, Javens J, Quardokus E, Brun Y. Mutations in DivL and CckA rescue a divJ null mutant of Caulobacter crescentus by reducing the activity of CtrA. J Bacteriol. 2006;188:2473-82 pubmed
    Polar development and cell division in Caulobacter crescentus are controlled and coordinated by multiple signal transduction proteins. divJ encodes a histidine kinase...
  39. Balhesteros H, Mazzon R, da Silva C, Lang E, Marques M. CspC and CspD are essential for Caulobacter crescentus stationary phase survival. Arch Microbiol. 2010;192:747-58 pubmed publisher
    ..b>Caulobacter crescentus cspA and cspB are induced upon cold shock, while cspC and cspD are induced during stationary phase...
  40. Chen Y, Tsokos C, Biondi E, Perchuk B, Laub M. Dynamics of two Phosphorelays controlling cell cycle progression in Caulobacter crescentus. J Bacteriol. 2009;191:7417-29 pubmed publisher
    In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA...
  41. Schredl A, Perez Mora Y, Herrera A, Cuajungco M, Murray S. The Caulobacter crescentus ctrA P1 promoter is essential for the coordination of cell cycle events that prevent the overinitiation of DNA replication. Microbiology. 2012;158:2492-503 pubmed publisher
    ..Thus, Caulobacter can adjust regulatory pathways to partially compensate for reduced and delayed CtrA accumulation in the ctrA P1 mutant...
  42. Corrêa J, Graciano L, Abrahão J, Loth E, Gandra R, Kadowaki M, et al. Expression and characterization of a GH39 ?-xylosidase II from Caulobacter crescentus. Appl Biochem Biotechnol. 2012;168:2218-29 pubmed publisher
    ..present work, the gene xynB2, encoding a ?-xylosidase II of the Glycoside Hydrolase 39 (GH39) family, of Caulobacter crescentus was cloned and successfully overexpressed in Escherichia coli DH10B...
  43. Tsokos C, Perchuk B, Laub M. A dynamic complex of signaling proteins uses polar localization to regulate cell-fate asymmetry in Caulobacter crescentus. Dev Cell. 2011;20:329-41 pubmed publisher
    ..Our results reveal the mechanisms by which CckA is regulated in a cell-type-dependent manner. More generally, our findings reveal how cells exploit subcellular localization to orchestrate sophisticated regulatory processes...
  44. Ireland M, Karty J, Quardokus E, Reilly J, Brun Y. Proteomic analysis of the Caulobacter crescentus stalk indicates competence for nutrient uptake. Mol Microbiol. 2002;45:1029-41 pubmed
    b>Caulobacter crescentus, a Gram-negative alpha-purple proteobacterium, is an oligotroph that lives in aquatic environments dilute in nutrients. This bacterium divides asymmetrically...
  45. Wagner J, Galvani C, Brun Y. Caulobacter crescentus requires RodA and MreB for stalk synthesis and prevention of ectopic pole formation. J Bacteriol. 2005;187:544-53 pubmed
    b>Caulobacter crescentus cells treated with amdinocillin, an antibiotic which specifically inhibits the cell elongation transpeptidase penicillin binding protein 2 in Escherichia coli, exhibit defects in stalk elongation and morphology, ..
  46. Judd E, Comolli L, Chen J, Downing K, Moerner W, McAdams H. Distinct constrictive processes, separated in time and space, divide caulobacter inner and outer membranes. J Bacteriol. 2005;187:6874-82 pubmed
    ..loss in photobleaching (FLIP) assay were used to characterize progression of the terminal stages of Caulobacter crescentus cell division...
  47. Huitema E, Pritchard S, Matteson D, Radhakrishnan S, Viollier P. Bacterial birth scar proteins mark future flagellum assembly site. Cell. 2006;124:1025-37 pubmed publisher
    Many prokaryotic protein complexes underlie polar asymmetry. In Caulobacter crescentus, a flagellum is built exclusively at the pole that arose from the previous cell division...
  48. McAdams H, Shapiro L. System-level design of bacterial cell cycle control. FEBS Lett. 2009;583:3984-91 pubmed publisher
    ..An essential phospho-signaling system integral to the cell cycle circuitry is central to accomplishing asymmetric cell division...
  49. White C, Kitich A, Gober J. Positioning cell wall synthetic complexes by the bacterial morphogenetic proteins MreB and MreD. Mol Microbiol. 2010;76:616-33 pubmed publisher
    In Caulobacter crescentus, intact cables of the actin homologue, MreB, are required for the proper spatial positioning of MurG which catalyses the final step in peptidoglycan precursor synthesis...
  50. Cole J, Hardy G, Bodenmiller D, Toh E, Hinz A, Brun Y. The HfaB and HfaD adhesion proteins of Caulobacter crescentus are localized in the stalk. Mol Microbiol. 2003;49:1671-83 pubmed
    The differentiating bacterium Caulobacter crescentus produces two different cell types at each cell division, a motile swarmer cell and an adhesive stalked cell...
  51. Llewellyn M, Dutton R, Easter J, O donnol D, Gober J. The conserved flaF gene has a critical role in coupling flagellin translation and assembly in Caulobacter crescentus. Mol Microbiol. 2005;57:1127-42 pubmed
    The expression of the flagellin proteins in Caulobacter crescentus is regulated by the progression of flagellar assembly both at the transcriptional and post-transcriptional levels...
  52. Benkovic S, Baker S, Alley M, Woo Y, Zhang Y, Akama T, et al. Identification of borinic esters as inhibitors of bacterial cell growth and bacterial methyltransferases, CcrM and MenH. J Med Chem. 2005;48:7468-76 pubmed
    ..These compounds were identified by screening for inhibitors against Caulobacter crescentus CcrM, an essential DNA methyltransferase from gram negative alpha-proteobacteria...
  53. Badger J, Hoover T, Brun Y, Weiner R, Laub M, Alexandre G, et al. Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus. J Bacteriol. 2006;188:6841-50 pubmed
    ..the only member of this group for which genome sequence was available was the model freshwater organism Caulobacter crescentus. Here we describe the genome sequence of Hyphomonas neptunium, a marine member of the DPB that differs ..
  54. Stephens C, Christen B, Fuchs T, Sundaram V, Watanabe K, Jenal U. Genetic analysis of a novel pathway for D-xylose metabolism in Caulobacter crescentus. J Bacteriol. 2007;189:2181-5 pubmed
    Genetic data suggest that the oligotrophic freshwater bacterium Caulobacter crescentus metabolizes D-xylose through a pathway yielding alpha-ketoglutarate, comparable to the recently described L-arabinose degradation pathway of ..
  55. Lawler M, Brun Y. Advantages and mechanisms of polarity and cell shape determination in Caulobacter crescentus. Curr Opin Microbiol. 2007;10:630-7 pubmed
    ..understanding of the mechanism and function of polarity and cell shape by studying the aquatic bacterium Caulobacter crescentus, whose cell cycle progression involves the ordered synthesis of different polar structures, and culminates ..
  56. Toro E, Hong S, McAdams H, Shapiro L. Caulobacter requires a dedicated mechanism to initiate chromosome segregation. Proc Natl Acad Sci U S A. 2008;105:15435-40 pubmed publisher
    ..We show that Caulobacter crescentus makes use of and requires a dedicated mechanism to initiate chromosome segregation...
  57. Angelastro P, Sliusarenko O, Jacobs Wagner C. Polar localization of the CckA histidine kinase and cell cycle periodicity of the essential master regulator CtrA in Caulobacter crescentus. J Bacteriol. 2010;192:539-52 pubmed publisher
    ..DNA replication initiation and regulates the transcription of about 100 cell cycle-regulated genes in Caulobacter crescentus. CtrA activity fluctuates during the cell cycle, and its periodicity is a key element of the engine that ..
  58. Abel S, Chien P, Wassmann P, Schirmer T, Kaever V, Laub M, et al. Regulatory cohesion of cell cycle and cell differentiation through interlinked phosphorylation and second messenger networks. Mol Cell. 2011;43:550-60 pubmed publisher
    In Caulobacter crescentus, phosphorylation of key regulators is coordinated with the second messenger cyclic di-GMP to drive cell-cycle progression and differentiation...
  59. Gora K, Cantin A, Wohlever M, Joshi K, Perchuk B, Chien P, et al. Regulated proteolysis of a transcription factor complex is critical to cell cycle progression in Caulobacter crescentus. Mol Microbiol. 2013;87:1277-89 pubmed publisher
    Cell cycle transitions are often triggered by the proteolysis of key regulatory proteins. In Caulobacter crescentus, the G1-S transition involves the degradation of an essential DNA-binding response regulator, CtrA, by the ClpXP protease...
  60. Berne C, Ma X, Licata N, Neves B, Setayeshgar S, Brun Y, et al. Physiochemical properties of Caulobacter crescentus holdfast: a localized bacterial adhesive. J Phys Chem B. 2013;117:10492-503 pubmed publisher
    To colonize surfaces, the bacterium Caulobacter crescentus employs a polar polysaccharide, the holdfast, located at the end of a thin, long stalk protruding from the cell body...
  61. da Silva Neto J, Lourenço R, Marques M. Global transcriptional response of Caulobacter crescentus to iron availability. BMC Genomics. 2013;14:549 pubmed publisher
    ..b>Caulobacter crescentus, an important freshwater ?-proteobacterium, uses the ferric uptake repressor (Fur) for such purpose...
  62. Guillet V, Ohta N, Cabantous S, Newton A, Samama J. Crystallographic and biochemical studies of DivK reveal novel features of an essential response regulator in Caulobacter crescentus. J Biol Chem. 2002;277:42003-10 pubmed publisher
    DivK is an essential response regulator in the Gram-negative bacterium Caulobacter crescentus and functions in a complex phosphorelay system that precisely controls the sequence of developmental events during the cell division cycle...
  63. Breier A, Cozzarelli N. Linear ordering and dynamic segregation of the bacterial chromosome. Proc Natl Acad Sci U S A. 2004;101:9175-6 pubmed
  64. Ryan K. Partners in crime: phosphotransfer profiling identifies a multicomponent phosphorelay. Mol Microbiol. 2006;59:361-3 pubmed
    The first multicomponent phosphorelay, regulating stalk biogenesis, has been identified in Caulobacter crescentus using a bioinformatic screen, targeted disruptions of each histidine kinase and response regulator, and a new technique ..
  65. Divakaruni A, Baida C, White C, Gober J. The cell shape proteins MreB and MreC control cell morphogenesis by positioning cell wall synthetic complexes. Mol Microbiol. 2007;66:174-88 pubmed
    ..In Caulobacter crescentus, MreC physically associates with penicillin-binding proteins (PBPs) which catalyse the insertion of ..
  66. Bowers L, Shapland E, Ryan K. Who's in charge here? Regulating cell cycle regulators. Curr Opin Microbiol. 2008;11:547-52 pubmed publisher
    ..key features of the transcriptional and signal transduction networks governing the cell division cycle of Caulobacter crescentus. These mechanisms generate oscillations in the activity of CtrA, a key regulator of DNA replication and ..
  67. Iniesta A, Hillson N, Shapiro L. Cell pole-specific activation of a critical bacterial cell cycle kinase. Proc Natl Acad Sci U S A. 2010;107:7012-7 pubmed publisher
    b>Caulobacter crescentus integrates phospho-signaling pathways and transcription factor regulatory cascades to drive the cell cycle...
  68. Moll A, Schlimpert S, Briegel A, Jensen G, Thanbichler M. DipM, a new factor required for peptidoglycan remodelling during cell division in Caulobacter crescentus. Mol Microbiol. 2010;77:90-107 pubmed publisher
    ..a thus far uncharacterized periplasmic protein, DipM, that is required for cell division and polarity in Caulobacter crescentus. DipM is composed of four peptidoglycan binding (LysM) domains and a C-terminal lysostaphin-like (LytM) ..
  69. Ptacin J, Lee S, Garner E, Toro E, Eckart M, Comolli L, et al. A spindle-like apparatus guides bacterial chromosome segregation. Nat Cell Biol. 2010;12:791-8 pubmed publisher
    ..Here we demonstrate that the bacterium Caulobacter crescentus segregates its chromosome using a partitioning (Par) apparatus that has surprising similarities to ..
  70. Bellefontaine A, Pierreux C, Mertens P, Vandenhaute J, Letesson J, De Bolle X. Plasticity of a transcriptional regulation network among alpha-proteobacteria is supported by the identification of CtrA targets in Brucella abortus. Mol Microbiol. 2002;43:945-60 pubmed
    CtrA is a master response regulator found in many alpha-proteobacteria. In Caulobacter crescentus and Sinorhizobium meliloti, this regulator is essential for viability and is transcriptionally autoregulated. In C...
  71. Radhakrishnan S, Thanbichler M, Viollier P. The dynamic interplay between a cell fate determinant and a lysozyme homolog drives the asymmetric division cycle of Caulobacter crescentus. Genes Dev. 2008;22:212-25 pubmed publisher
    b>Caulobacter crescentus divides asymmetrically into a swarmer cell and a stalked cell, a process that is governed by the imbalance in phosphorylated levels of the DivK cell fate determinant in the two cellular compartments...
  72. Siegal Gaskins D, Ash J, Crosson S. Model-based deconvolution of cell cycle time-series data reveals gene expression details at high resolution. PLoS Comput Biol. 2009;5:e1000460 pubmed publisher
    ..Applying our deconvolution algorithm to cell cycle gene expression data from the dimorphic bacterium Caulobacter crescentus, we recovered critical features of cell cycle regulation in essential genes, including ctrA and ftsZ, that ..
  73. Herrou J, Foreman R, Fiebig A, Crosson S. A structural model of anti-anti-? inhibition by a two-component receiver domain: the PhyR stress response regulator. Mol Microbiol. 2010;78:290-304 pubmed publisher
    ..We present genetic evidence that Caulobacter crescentus PhyR is a phosphorylation-dependent stress regulator that functions in the same pathway as ?(T) and its ..
  74. Muir R, Gober J. Mutations in FlbD that relieve the dependency on flagellum assembly alter the temporal and spatial pattern of developmental transcription in Caulobacter crescentus. Mol Microbiol. 2002;43:597-615 pubmed
    ..factor FlbD regulates the temporal and spatial transcription of flagellar genes in the bacterium Caulobacter crescentus. Activation of FlbD requires cell cycle progression and the assembly of an early (class II) flagellum ..
  75. Ackermann M, Stearns S, Jenal U. Senescence in a bacterium with asymmetric division. Science. 2003;300:1920 pubmed
  76. Iniesta A, McGrath P, Reisenauer A, McAdams H, Shapiro L. A phospho-signaling pathway controls the localization and activity of a protease complex critical for bacterial cell cycle progression. Proc Natl Acad Sci U S A. 2006;103:10935-40 pubmed
    ..Dynamic protease localization mediated by a phospho-signaling pathway is a novel mechanism to integrate spatial and temporal control of bacterial cell cycle progression...
  77. Ford M, Nomellini J, Smit J. S-layer anchoring and localization of an S-layer-associated protease in Caulobacter crescentus. J Bacteriol. 2007;189:2226-37 pubmed publisher
    The S-layer of the gram-negative bacterium Caulobacter crescentus is composed of a single protein, RsaA, that is secreted and assembled into a hexagonal crystalline array that covers the organism...
  78. Hillson N, Hu P, Andersen G, Shapiro L. Caulobacter crescentus as a whole-cell uranium biosensor. Appl Environ Microbiol. 2007;73:7615-21 pubmed
    We engineered a strain of the bacterium Caulobacter crescentus to fluoresce in the presence of micromolar levels of uranium at ambient temperatures when it is exposed to a hand-held UV lamp...
  79. Toh E, Kurtz H, Brun Y. Characterization of the Caulobacter crescentus holdfast polysaccharide biosynthesis pathway reveals significant redundancy in the initiating glycosyltransferase and polymerase steps. J Bacteriol. 2008;190:7219-31 pubmed publisher
    b>Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine...
  80. Panis G, Lambert C, Viollier P. Complete genome sequence of Caulobacter crescentus bacteriophage ?CbK. J Virol. 2012;86:10234-5 pubmed publisher
    CbK is a B3 morphotype bacteriophage of the Siphoviridae family that infects Caulobacter crescentus, the preeminent model system for bacterial cell cycle studies...
  81. Judd E, Ryan K, Moerner W, Shapiro L, McAdams H. Fluorescence bleaching reveals asymmetric compartment formation prior to cell division in Caulobacter. Proc Natl Acad Sci U S A. 2003;100:8235-40 pubmed
    Asymmetric cell division in Caulobacter crescentus yields daughter cells that have different cell fates...
  82. Lo T, van Der Schalie E, Werner T, Brun Y, Din N. A temperature-sensitive mutation in the dnaE gene of Caulobacter crescentus that prevents initiation of DNA replication but not ongoing elongation of DNA. J Bacteriol. 2004;186:1205-12 pubmed
    A genetic screen for cell division cycle mutants of Caulobacter crescentus identified a temperature-sensitive DNA replication mutant...
  83. Muir R, Gober J. Regulation of FlbD activity by flagellum assembly is accomplished through direct interaction with the trans-acting factor, FliX. Mol Microbiol. 2004;54:715-30 pubmed publisher
    The temporal and spatial transcription of late flagellar genes in Caulobacter crescentus is regulated by the sigma54 transcriptional activator, FlbD...
  84. Lam H, Schofield W, Jacobs Wagner C. A landmark protein essential for establishing and perpetuating the polarity of a bacterial cell. Cell. 2006;124:1011-23 pubmed publisher
    ..TipN, which acts as a spatial and temporal cue for setting up the correct polarity in the bacterium Caulobacter crescentus. TipN marks the new pole throughout most of the cell cycle, and its relocation to the nascent poles at the ..
  85. Poggio S, Takacs C, Vollmer W, Jacobs Wagner C. A protein critical for cell constriction in the Gram-negative bacterium Caulobacter crescentus localizes at the division site through its peptidoglycan-binding LysM domains. Mol Microbiol. 2010;77:74-89 pubmed publisher
    ..Here we identify and characterize a member of this family (DipM) in Caulobacter crescentus. Unlike its E. coli counterparts, DipM is essential for viability under fast-growth conditions...
  86. Gill J, Berry J, Russell W, Lessor L, Escobar Garcia D, Hernandez D, et al. The Caulobacter crescentus phage phiCbK: genomics of a canonical phage. BMC Genomics. 2012;13:542 pubmed publisher
    The bacterium Caulobacter crescentus is a popular model for the study of cell cycle regulation and senescence. The large prolate siphophage phiCbK has been an important tool in C...
  87. Gonzalez D, Collier J. DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus. Mol Microbiol. 2013;88:203-18 pubmed publisher
    ..In this study, we find that Caulobacter crescentus cells lacking the CcrM enzyme accumulate low levels of the two conserved FtsZ and MipZ proteins, leading ..
  88. Valencia E, Braz V, Guzzo C, Marques M. Two RND proteins involved in heavy metal efflux in Caulobacter crescentus belong to separate clusters within proteobacteria. BMC Microbiol. 2013;13:79 pubmed publisher
    ..These proteins constitute the cytoplasmic membrane channel of the tripartite RND transport systems. Caulobacter crescentus NA1000 possess two HME-RND proteins, and the aim of this work was to determine their involvement in the ..
  89. Cabantous S, Guillet V, Ohta N, Newton A, Samama J. Characterization and crystallization of DivK, an essential response regulator for cell division and differentiation in Caulobacter crescentus. Acta Crystallogr D Biol Crystallogr. 2002;58:1249-51 pubmed
    ..involved in the complex signal transduction network required for cell division and cell differentiation in Caulobacter crescentus. Small-angle X-ray scattering analysis was valuable for obtaining single crystals of the DivK recombinant ..
  90. Figge R, Divakaruni A, Gober J. MreB, the cell shape-determining bacterial actin homologue, co-ordinates cell wall morphogenesis in Caulobacter crescentus. Mol Microbiol. 2004;51:1321-32 pubmed
    ..The depletion of MreB in Caulobacter crescentus resulted in lemon-shaped cells that possessed defects in the integrity of the cell wall...
  91. Stephens C, Christen B, Watanabe K, Fuchs T, Jenal U. Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressor. J Bacteriol. 2007;189:8828-34 pubmed
    In the oligotrophic freshwater bacterium Caulobacter crescentus, D-xylose induces expression of over 50 genes, including the xyl operon, which encodes key enzymes for xylose metabolism...
  92. Purcell E, Siegal Gaskins D, Rawling D, Fiebig A, Crosson S. A photosensory two-component system regulates bacterial cell attachment. Proc Natl Acad Sci U S A. 2007;104:18241-6 pubmed
    ..The differentiating bacterium, Caulobacter crescentus, contains an operon encoding a two-component signaling system consisting of a LOV-histidine kinase, LovK, ..